Rroxscaffold_2G00139110

plant-type hypersensitive response

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014069
Physical Location & Seq
Reverse (-)
77395239 .. 77398273
3035 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00139110.1

Sequence Viewer

Length: 579 bp
ATGGAACAACTCTTTATAGAGGCTACAATAGGACATGCACTAGAGGCCTTATTGAGAGCTGTTTCACAAGCCAGGGACAGAGCGCACAACTATGAGTCCATTCTTGCACAACTTGAATTCACAATCCAATCCATCGCTCCAAAAATCTATGAAATCGATTGTTTATCTGAAGAACATCTCCAGGATGATGTGGTCCACCGAATACGTCTTCAGCTAATTCAAGGTAAGGAGTTAGTCGATAAGTGTTCAAAGTTGGGTTGCTGCACCTTCTACAACAGCCAGAGGTACTACAAGAAGCTCCAAGAGTTGGATATTTCTCTTGGAAGGCTGATTACGATTGACTTGCAAGTTAAGGTGGCGTTGGATGTTGTAAGGATTTCGGCTGACATGAAAGAGCTCTGTAAGAGATGGGATGATCAAGTGAAGAATGTACATGAAGCGGGAGGACAGAGTACTTGGATTTTCAAGCTAAGTGGAAGAAAGCTTCTGGGTAGGTTTGCTGGACTAAAGAAGATTGTATTGTACAAACCAAAGAGAATCCTTCTGAATTTGGTTACTTGTGGAGCTATGGAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0043207 GO:0050896 GO:0051704 GO:0051707
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPW8

Protein Analysis

192

Amino Acids

22.0

Weight (kDa)

8.48

Isoelectric Point (pI)

39.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 8 - 134 4e-25 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014069)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50450 AT3G50460 AT3G50470 AT3G50480 AT3G50480
malus_domestica MD02G1163800.v1.1
prunus_persica Prupe.7G140100_v2.0.a1
rosa_laevigata RLG00000017290
rosa_multiflora Rmu_ssc0000257.1_g000011
rosa_roxburghii Rroxscaffold_2G00139110
rosa_rugosa Rorug02G0119300
rosa_samantha Rh2AG168800 Rh2BG176000 Rh2CG174400 Rh2DG174300 Rh6DG281400
rosa_wichuraiana Rw2G013210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 307
AciI CCGC 1 cut(s) 440
AcsI RAATTY 2 cut(s) 116, 547
AcuI CTGAAG 2 cut(s) 189, 194
AfaI GTAC 4 cut(s) 287, 432, 454, 524
AfiI CCNNNNNNNGG 1 cut(s) 307
AgsI TTSAA 4 cut(s) 116, 221, 249, 466
AjnI CCWGG 2 cut(s) 71, 180
AluBI AGCT 7 cut(s) 59, 214, 298, 397, 469, 484, 566
AluI AGCT 7 cut(s) 59, 214, 298, 397, 469, 484, 566
Alw21I GWGCWC 1 cut(s) 399
AoxI GGCC 1 cut(s) 45
ApeKI GCWGC 1 cut(s) 261
ApoI RAATTY 2 cut(s) 116, 547
AspLEI GCGC 1 cut(s) 85
AspS9I GGNCC 1 cut(s) 193
AvaII GGWCC 1 cut(s) 193
BanII GRGCYC 1 cut(s) 399
BarI GAAGNNNNNNTAC 2 cut(s) 316, 348
BbsI GAAGAC 1 cut(s) 200
Bbv12I GWGCWC 1 cut(s) 399
BbvI GCAGC 1 cut(s) 248
BccI CCATC 2 cut(s) 140, 402
BcgI CGANNNNNNTGC 2 cut(s) 325, 359
BciT130I CCWGG 2 cut(s) 73, 182
BclI TGATCA 1 cut(s) 415
BfaI CTAG 1 cut(s) 41
BisI GCNGC 1 cut(s) 262
BlsI GCNGC 1 cut(s) 263
BmcAI AGTACT 1 cut(s) 454
Bme1390I CCNGG 2 cut(s) 73, 182
Bme18I GGWCC 1 cut(s) 193
BmgT120I GGNCC 1 cut(s) 193
BmrFI CCNGG 2 cut(s) 73, 182
BpiI GAAGAC 1 cut(s) 200
BpmI CTGGAG 1 cut(s) 164
Bsa29I ATCGAT 1 cut(s) 156
BsaJI CCNNGG 1 cut(s) 72
Bsc4I CCNNNNNNNGG 1 cut(s) 307
BseBI CCWGG 2 cut(s) 73, 182
BseCI ATCGAT 1 cut(s) 156
BseDI CCNNGG 1 cut(s) 72
BseGI GGATG 3 cut(s) 190, 370, 418
BseLI CCNNNNNNNGG 1 cut(s) 307
BseXI GCAGC 1 cut(s) 248
BsgI GTGCAG 1 cut(s) 247
BshFI GGCC 1 cut(s) 47
BshVI ATCGAT 1 cut(s) 156
BsiHKAI GWGCWC 1 cut(s) 399
BslFI GGGAC 1 cut(s) 89
BslI CCNNNNNNNGG 1 cut(s) 307
BsmFI GGGAC 1 cut(s) 89
BsnI GGCC 1 cut(s) 47
Bsp1286I GDGCHC 1 cut(s) 399
Bsp1407I TGTACA 2 cut(s) 430, 522
Bsp143I GATC 1 cut(s) 415
BspACI CCGC 1 cut(s) 440
BspANI GGCC 1 cut(s) 47
BspDI ATCGAT 1 cut(s) 156
BsrGI TGTACA 2 cut(s) 430, 522
BssECI CCNNGG 1 cut(s) 72
BssMI GATC 1 cut(s) 415
Bst2UI CCWGG 2 cut(s) 73, 182
BstAUI TGTACA 2 cut(s) 430, 522
BstDEI CTNAG 1 cut(s) 470
BstF5I GGATG 3 cut(s) 190, 370, 418
BstHHI GCGC 1 cut(s) 85
BstKTI GATC 1 cut(s) 418
BstMBI GATC 1 cut(s) 415
BstMWI GCNNNNNNNGC 1 cut(s) 44
BstNI CCWGG 2 cut(s) 73, 182
BstNSI RCATGY 1 cut(s) 38
BstSCI CCNGG 2 cut(s) 71, 180
BstV1I GCAGC 1 cut(s) 248
BstV2I GAAGAC 1 cut(s) 200
Bsu15I ATCGAT 1 cut(s) 156
BsuRI GGCC 1 cut(s) 47
BsuTUI ATCGAT 1 cut(s) 156
BtgZI GCGATG 1 cut(s) 118
BtsCI GGATG 3 cut(s) 190, 370, 418
CfoI GCGC 1 cut(s) 85
Cfr13I GGNCC 1 cut(s) 193
ClaI ATCGAT 1 cut(s) 156
Csp6I GTAC 4 cut(s) 286, 431, 453, 523
CspCI CAANNNNNGTGG 2 cut(s) 454, 489
CviAII CATG 3 cut(s) 35, 388, 434
CviQI GTAC 4 cut(s) 286, 431, 453, 523
DdeI CTNAG 1 cut(s) 470
DpnI GATC 1 cut(s) 417
DpnII GATC 1 cut(s) 415
Ecl136II GAGCTC 1 cut(s) 397
Eco147I AGGCCT 1 cut(s) 47
Eco24I GRGCYC 1 cut(s) 399
Eco47I GGWCC 1 cut(s) 193
Eco53kI GAGCTC 1 cut(s) 397
Eco57I CTGAAG 2 cut(s) 189, 194
EcoICRI GAGCTC 1 cut(s) 397
EcoRI GAATTC 1 cut(s) 116
EcoRII CCWGG 2 cut(s) 71, 180
EcoT38I GRGCYC 1 cut(s) 399
FaeI CATG 3 cut(s) 38, 391, 437
FaiI YATR 7 cut(s) 17, 36, 93, 150, 389, 435, 569
FaqI GGGAC 1 cut(s) 89
FatI CATG 3 cut(s) 34, 387, 433
FauI CCCGC 1 cut(s) 433
FbaI TGATCA 1 cut(s) 415
Fnu4HI GCNGC 1 cut(s) 262
FokI GGATG 3 cut(s) 197, 377, 425
FriOI GRGCYC 1 cut(s) 399
Fsp4HI GCNGC 1 cut(s) 262
FspBI CTAG 1 cut(s) 41
GlaI GCGC 1 cut(s) 84
GluI GCNGC 1 cut(s) 262
GsuI CTGGAG 1 cut(s) 164
HaeIII GGCC 1 cut(s) 47
HhaI GCGC 1 cut(s) 85
Hin1II CATG 3 cut(s) 38, 391, 437
Hin6I GCGC 1 cut(s) 83
HinP1I GCGC 1 cut(s) 83
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 2 cut(s) 95, 537
Hpy166II GTNNAC 1 cut(s) 196
Hpy188I TCNGA 2 cut(s) 169, 546
Hpy8I GTNNAC 1 cut(s) 196
HpyAV CCTTC 3 cut(s) 277, 318, 551
HpyCH4IV ACGT 1 cut(s) 205
HpyCH4V TGCA 4 cut(s) 38, 107, 264, 346
HpyF10VI GCNNNNNNNGC 1 cut(s) 44
HpyF3I CTNAG 1 cut(s) 470
HpySE526I ACGT 1 cut(s) 205
Hsp92II CATG 3 cut(s) 38, 391, 437
HspAI GCGC 1 cut(s) 83
Ksp22I TGATCA 1 cut(s) 415
Kzo9I GATC 1 cut(s) 415
LmnI GCTCC 3 cut(s) 142, 303, 563
LpnPI CCDG 7 cut(s) 58, 85, 167, 194, 293, 473, 486
Lsp1109I GCAGC 1 cut(s) 248
MaeI CTAG 1 cut(s) 41
MaeII ACGT 1 cut(s) 205
MaeIII GTNAC 1 cut(s) 553
MalI GATC 1 cut(s) 417
MboI GATC 1 cut(s) 415
MboII GAAGA 5 cut(s) 182, 200, 436, 489, 523
MhlI GDGCHC 1 cut(s) 399
MluCI AATT 3 cut(s) 116, 216, 547
MlyI GAGTC 1 cut(s) 104
MmeI TCCRAC 2 cut(s) 288, 342
MnlI CCTC 4 cut(s) 13, 37, 276, 437
MseI TTAA 2 cut(s) 351, 577
MslI CAYNNNNRTG 1 cut(s) 90
MspR9I CCNGG 2 cut(s) 73, 182
MvaI CCWGG 2 cut(s) 73, 182
MwoI GCNNNNNNNGC 1 cut(s) 44
NdeII GATC 1 cut(s) 415
NlaIII CATG 3 cut(s) 38, 391, 437
NspI RCATGY 1 cut(s) 38
PceI AGGCCT 1 cut(s) 47
PfeI GAWTC 1 cut(s) 537
PflMI CCANNNNNTGG 1 cut(s) 307
PfoI TCCNGGA 1 cut(s) 180
PkrI GCNGC 1 cut(s) 263
PleI GAGTC 1 cut(s) 103
PpsI GAGTC 1 cut(s) 103
Psp124BI GAGCTC 1 cut(s) 399
Psp6I CCWGG 2 cut(s) 71, 180
PspGI CCWGG 2 cut(s) 71, 180
PspPI GGNCC 1 cut(s) 193
RsaI GTAC 4 cut(s) 287, 432, 454, 524
RsaNI GTAC 4 cut(s) 286, 431, 453, 523
RseI CAYNNNNRTG 1 cut(s) 90
SacI GAGCTC 1 cut(s) 399
SaqAI TTAA 2 cut(s) 351, 577
SatI GCNGC 1 cut(s) 262
Sau3AI GATC 1 cut(s) 415
Sau96I GGNCC 1 cut(s) 193
ScaI AGTACT 1 cut(s) 454
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 2 cut(s) 73, 182
SduI GDGCHC 1 cut(s) 399
SinI GGWCC 1 cut(s) 193
SmiMI CAYNNNNRTG 1 cut(s) 90
Sse9I AATT 3 cut(s) 116, 216, 547
SseBI AGGCCT 1 cut(s) 47
SsiI CCGC 1 cut(s) 440
SspMI CTAG 1 cut(s) 41
SstI GAGCTC 1 cut(s) 399
StuI AGGCCT 1 cut(s) 47
StyD4I CCNGG 2 cut(s) 71, 180
TaiI ACGT 1 cut(s) 208
TaqI TCGA 2 cut(s) 156, 237
TasI AATT 3 cut(s) 116, 216, 547
TatI WGTACW 3 cut(s) 430, 452, 522
TfiI GAWTC 1 cut(s) 537
Tru1I TTAA 2 cut(s) 351, 577
Tru9I TTAA 2 cut(s) 351, 577
TseI GCWGC 1 cut(s) 261
TspDTI ATGAA 3 cut(s) 165, 404, 450
Van91I CCANNNNNTGG 1 cut(s) 307
VpaK11BI GGWCC 1 cut(s) 193
XapI RAATTY 2 cut(s) 116, 547
XceI RCATGY 1 cut(s) 38
XspI CTAG 1 cut(s) 41
ZrmI AGTACT 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.