MD02G1163800.v1.1

plant-type hypersensitive response

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014069
Physical Location & Seq
Forward (+)
14008908 .. 14009492
585 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1163800.v1.1.491

Sequence Viewer

Length: 585 bp
ATGGAAAAAATTCTTCTAGATGCTACAATAGGACGCGCACTGGAGGCGCTACTAGGAGCTGTCTTGCAAGCCAGGGAGAGAGCGCATATGTATGAGTCTATTCTTGCACAGTTAGAATTCACGATTCATACAATAACTCCAAAAATCAGTGACATCGACCGTCTTTCTGACGAAGATTTCCCAGACGATGTCATCCATCGAATACGCCTACAGCTGATTCGAGGGAAGGAGCTAGTCGATAAGTGCTCGAATGCAGGTGGCTGCAACTTCTGCAAGGGGCAAAGGTACTACAAGAAGCTCCTCGAGTTGGACGAGTCTCTTCGGAGGCTGATTACTATTGATTTGCAAATTAAGGTGGCATTGGATGTTGTAAGGATTTCAAATGAGATGAAGGCAATATGTAGGAGATGGGATCAAATGGATGTGAATGGAGGAGGTGGGAGGGGTTGGATTTTCAATTTAAGTGGAAGTAGGGTTTTGGGTATTAAGTTTTCCGGACTGAAGAGGTTTGTGCAATCTGTGGAGTCAGCACCAAAGAGAATCCTTATAAATTTGGTTACATGTGGGGCAATTACAGGTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0043207 GO:0050896 GO:0051704 GO:0051707
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPW8

Protein Analysis

195

Amino Acids

21.83

Weight (kDa)

9.02

Isoelectric Point (pI)

34.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 8 - 133 4.8e-27 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014069)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50450 AT3G50460 AT3G50470 AT3G50480 AT3G50480
malus_domestica MD02G1163800.v1.1
prunus_persica Prupe.7G140100_v2.0.a1
rosa_laevigata RLG00000017290
rosa_multiflora Rmu_ssc0000257.1_g000011
rosa_roxburghii Rroxscaffold_2G00139110
rosa_rugosa Rorug02G0119300
rosa_samantha Rh2AG168800 Rh2BG176000 Rh2CG174400 Rh2DG174300 Rh6DG281400
rosa_wichuraiana Rw2G013210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 548
AarI CACCTGC 1 cut(s) 245
Acc36I ACCTGC 1 cut(s) 245
AccII CGCG 1 cut(s) 36
AccIII TCCGGA 1 cut(s) 494
AclWI GGATC 1 cut(s) 420
AcsI RAATTY 3 cut(s) 9, 116, 550
AcuI CTGAAG 1 cut(s) 521
AfaI GTAC 1 cut(s) 287
AfiI CCNNNNNNNGG 1 cut(s) 307
AflIII ACRYGT 1 cut(s) 560
AgsI TTSAA 2 cut(s) 381, 457
AjnI CCWGG 1 cut(s) 71
AluBI AGCT 4 cut(s) 59, 214, 232, 298
AluI AGCT 4 cut(s) 59, 214, 232, 298
Alw21I GWGCWC 1 cut(s) 248
Alw26I GTCTC 1 cut(s) 321
AlwI GGATC 1 cut(s) 420
Ama87I CYCGRG 1 cut(s) 302
Aor13HI TCCGGA 1 cut(s) 494
ApeKI GCWGC 1 cut(s) 261
ApoI RAATTY 3 cut(s) 9, 116, 550
Asp700I GAANNNNTTC 1 cut(s) 9
AspLEI GCGC 3 cut(s) 38, 49, 85
AvaI CYCGRG 1 cut(s) 302
Bbv12I GWGCWC 1 cut(s) 248
BbvI GCAGC 1 cut(s) 248
BccI CCATC 2 cut(s) 204, 402
BciT130I CCWGG 1 cut(s) 73
BcoDI GTCTC 1 cut(s) 321
BfaI CTAG 3 cut(s) 17, 53, 233
BfmI CTRYAG 1 cut(s) 209
BfoI RGCGCY 1 cut(s) 50
BfuAI ACCTGC 1 cut(s) 245
BisI GCNGC 1 cut(s) 262
BlsI GCNGC 1 cut(s) 263
Bme1390I CCNGG 1 cut(s) 73
BmeT110I CYCGRG 1 cut(s) 302
BmrFI CCNGG 1 cut(s) 73
BmsI GCATC 1 cut(s) 10
BpmI CTGGAG 1 cut(s) 62
BsaJI CCNNGG 1 cut(s) 72
BsaWI WCCGGW 1 cut(s) 494
BsaXI ACNNNNNCTCC 2 cut(s) 121, 151
Bsc4I CCNNNNNNNGG 1 cut(s) 307
Bse1I ACTGG 1 cut(s) 45
BseAI TCCGGA 1 cut(s) 494
BseBI CCWGG 1 cut(s) 73
BseDI CCNNGG 1 cut(s) 72
BseGI GGATG 3 cut(s) 192, 370, 427
BseLI CCNNNNNNNGG 1 cut(s) 307
BseNI ACTGG 1 cut(s) 45
BseRI GAGGAG 2 cut(s) 290, 447
BseXI GCAGC 1 cut(s) 248
Bsh1236I CGCG 1 cut(s) 36
Bsh1285I CGRYCG 1 cut(s) 160
BsiEI CGRYCG 1 cut(s) 160
BsiHKAI GWGCWC 1 cut(s) 248
BsiHKCI CYCGRG 1 cut(s) 302
BsiSI CCGG 1 cut(s) 495
BslI CCNNNNNNNGG 1 cut(s) 307
BsmAI GTCTC 1 cut(s) 321
BsmI GAATGC 1 cut(s) 256
BsoBI CYCGRG 1 cut(s) 302
Bsp1286I GDGCHC 1 cut(s) 248
Bsp13I TCCGGA 1 cut(s) 494
Bsp143I GATC 1 cut(s) 412
BspEI TCCGGA 1 cut(s) 494
BspFNI CGCG 1 cut(s) 36
BspMI ACCTGC 1 cut(s) 245
BspPI GGATC 1 cut(s) 420
BsrI ACTGG 1 cut(s) 45
BssECI CCNNGG 1 cut(s) 72
BssMI GATC 1 cut(s) 412
Bst2UI CCWGG 1 cut(s) 73
Bst4CI ACNGT 2 cut(s) 111, 161
Bst6I CTCTTC 2 cut(s) 324, 497
BstAPI GCANNNNNTGC 1 cut(s) 270
BstC8I GCNNGC 1 cut(s) 69
BstF5I GGATG 3 cut(s) 192, 370, 427
BstFNI CGCG 1 cut(s) 36
BstH2I RGCGCY 1 cut(s) 50
BstHHI GCGC 3 cut(s) 38, 49, 85
BstKTI GATC 1 cut(s) 415
BstMAI GTCTC 1 cut(s) 321
BstMBI GATC 1 cut(s) 412
BstMCI CGRYCG 1 cut(s) 160
BstMWI GCNNNNNNNGC 2 cut(s) 44, 270
BstNI CCWGG 1 cut(s) 73
BstNSI RCATGY 1 cut(s) 564
BstSCI CCNGG 1 cut(s) 71
BstSFI CTRYAG 1 cut(s) 209
BstUI CGCG 1 cut(s) 36
BstV1I GCAGC 1 cut(s) 248
BtsCI GGATG 3 cut(s) 192, 370, 427
BtsIMutI CAGTG 2 cut(s) 38, 154
BveI ACCTGC 1 cut(s) 245
Cac8I GCNNGC 1 cut(s) 69
CfoI GCGC 3 cut(s) 38, 49, 85
CseI GACGC 1 cut(s) 42
Csp6I GTAC 1 cut(s) 286
CspCI CAANNNNNGTGG 2 cut(s) 445, 480
CviAII CATG 1 cut(s) 561
CviJI RGCY 7 cut(s) 59, 71, 214, 232, 261, 298, 328
CviKI_1 RGCY 7 cut(s) 59, 71, 214, 232, 261, 298, 328
CviQI GTAC 1 cut(s) 286
DpnI GATC 1 cut(s) 414
DpnII GATC 1 cut(s) 412
Eam1104I CTCTTC 2 cut(s) 324, 497
EarI CTCTTC 2 cut(s) 324, 497
Eco57I CTGAAG 1 cut(s) 521
Eco88I CYCGRG 1 cut(s) 302
EcoRI GAATTC 1 cut(s) 116
EcoRII CCWGG 1 cut(s) 71
FaeI CATG 1 cut(s) 564
FaiI YATR 7 cut(s) 87, 89, 93, 129, 400, 548, 562
FatI CATG 1 cut(s) 560
FauNDI CATATG 1 cut(s) 87
Fnu4HI GCNGC 1 cut(s) 262
FokI GGATG 3 cut(s) 179, 377, 434
Fsp4HI GCNGC 1 cut(s) 262
FspBI CTAG 3 cut(s) 17, 53, 233
GlaI GCGC 3 cut(s) 37, 48, 84
GluI GCNGC 1 cut(s) 262
GsuI CTGGAG 1 cut(s) 62
HaeII RGCGCY 1 cut(s) 50
HapII CCGG 1 cut(s) 495
HgaI GACGC 1 cut(s) 42
HhaI GCGC 3 cut(s) 38, 49, 85
Hin1II CATG 1 cut(s) 564
Hin6I GCGC 3 cut(s) 36, 47, 83
HinP1I GCGC 3 cut(s) 36, 47, 83
HinfI GANTC 6 cut(s) 95, 124, 217, 314, 524, 540
HpaII CCGG 1 cut(s) 495
Hpy188I TCNGA 2 cut(s) 169, 324
Hpy188III TCNNGA 3 cut(s) 17, 121, 495
HpyAV CCTTC 2 cut(s) 220, 385
HpyCH4III ACNGT 2 cut(s) 111, 161
HpyCH4V TGCA 7 cut(s) 67, 107, 254, 264, 273, 346, 514
HpyF10VI GCNNNNNNNGC 2 cut(s) 44, 270
Hsp92II CATG 1 cut(s) 564
HspAI GCGC 3 cut(s) 36, 47, 83
Kpn2I TCCGGA 1 cut(s) 494
Kzo9I GATC 1 cut(s) 412
LmnI GCTCC 3 cut(s) 56, 229, 303
LpnPI CCDG 7 cut(s) 26, 58, 85, 195, 240, 508, 561
Lsp1109I GCAGC 1 cut(s) 248
LweI GCATC 1 cut(s) 10
MaeI CTAG 3 cut(s) 17, 53, 233
MaeIII GTNAC 2 cut(s) 149, 556
MalI GATC 1 cut(s) 414
MboI GATC 1 cut(s) 412
MboII GAAGA 4 cut(s) 5, 185, 311, 514
MhlI GDGCHC 1 cut(s) 248
MluCI AATT 6 cut(s) 9, 116, 348, 457, 550, 570
MlyI GAGTC 3 cut(s) 104, 323, 533
MmeI TCCRAC 2 cut(s) 288, 428
MnlI CCTC 8 cut(s) 37, 215, 311, 318, 425, 428, 435, 498
MroI TCCGGA 1 cut(s) 494
MroXI GAANNNNTTC 1 cut(s) 9
MseI TTAA 3 cut(s) 351, 461, 486
MslI CAYNNNNRTG 1 cut(s) 90
MspA1I CMGCKG 1 cut(s) 214
MspI CCGG 1 cut(s) 495
MspR9I CCNGG 1 cut(s) 73
Mva1269I GAATGC 1 cut(s) 256
MvaI CCWGG 1 cut(s) 73
MvnI CGCG 1 cut(s) 36
MwoI GCNNNNNNNGC 2 cut(s) 44, 270
NdeI CATATG 1 cut(s) 87
NdeII GATC 1 cut(s) 412
NlaIII CATG 1 cut(s) 564
NmuCI GTSAC 1 cut(s) 149
NspI RCATGY 1 cut(s) 564
PaeR7I CTCGAG 1 cut(s) 302
PaqCI CACCTGC 1 cut(s) 245
PciI ACATGT 1 cut(s) 560
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PctI GAATGC 1 cut(s) 256
PdmI GAANNNNTTC 1 cut(s) 9
PfeI GAWTC 3 cut(s) 124, 217, 540
PflFI GACNNNGTC 1 cut(s) 188
PkrI GCNGC 1 cut(s) 263
PleI GAGTC 3 cut(s) 103, 322, 532
PpsI GAGTC 3 cut(s) 103, 322, 532
PscI ACATGT 1 cut(s) 560
PsiI TTATAA 1 cut(s) 548
Psp6I CCWGG 1 cut(s) 71
PspGI CCWGG 1 cut(s) 71
PspXI VCTCGAGB 1 cut(s) 302
PsyI GACNNNGTC 1 cut(s) 188
PvuII CAGCTG 1 cut(s) 214
RsaI GTAC 1 cut(s) 287
RsaNI GTAC 1 cut(s) 286
RseI CAYNNNNRTG 1 cut(s) 90
SaqAI TTAA 3 cut(s) 351, 461, 486
SatI GCNGC 1 cut(s) 262
Sau3AI GATC 1 cut(s) 412
SchI GAGTC 3 cut(s) 104, 323, 533
ScrFI CCNGG 1 cut(s) 73
SduI GDGCHC 1 cut(s) 248
SfaNI GCATC 1 cut(s) 10
SfcI CTRYAG 1 cut(s) 209
Sfr274I CTCGAG 1 cut(s) 302
SlaI CTCGAG 1 cut(s) 302
SmiMI CAYNNNNRTG 1 cut(s) 90
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
Sse9I AATT 6 cut(s) 9, 116, 348, 457, 550, 570
SspMI CTAG 3 cut(s) 17, 53, 233
StyD4I CCNGG 1 cut(s) 71
TaaI ACNGT 2 cut(s) 111, 161
TaqI TCGA 6 cut(s) 156, 199, 220, 237, 248, 303
TasI AATT 6 cut(s) 9, 116, 348, 457, 550, 570
TfiI GAWTC 3 cut(s) 124, 217, 540
Tru1I TTAA 3 cut(s) 351, 461, 486
Tru9I TTAA 3 cut(s) 351, 461, 486
TscAI CASTG 2 cut(s) 45, 154
TseFI GTSAC 1 cut(s) 149
TseI GCWGC 1 cut(s) 261
Tsp45I GTSAC 1 cut(s) 149
TspDTI ATGAA 2 cut(s) 116, 404
TspRI CASTG 2 cut(s) 45, 154
Tth111I GACNNNGTC 1 cut(s) 188
XapI RAATTY 3 cut(s) 9, 116, 550
XbaI TCTAGA 1 cut(s) 16
XceI RCATGY 1 cut(s) 564
XhoI CTCGAG 1 cut(s) 302
XmnI GAANNNNTTC 1 cut(s) 9
XspI CTAG 3 cut(s) 17, 53, 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.