AT3G50470

plant-type hypersensitive response

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014069
Physical Location & Seq
Forward (+)
18728935 .. 18730497
1563 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G50470.1

Sequence Viewer

Length: 642 bp
ATGCCGGTTAGTGAGATTATGGCAGGGGCAGCTCTTGGACTTGCTCTCCAAGTCCTTCACGATGCCATCAAAAAAGCAAAAGATAGATCTCTAACTACAAGATGCATCTTGGACCGTCTTGATGCTACAATCTTTAGGATCACTCCGTTGGTGACTCAAGTCGATAAGCTTAGCGAAGAAGTGGAAGATTCACCGAGGAAAGTCATCGAAGATCTTAAGCATCTCCTTGAAAAGGCTGTTTCTCTTGTTGAGGCTTATGCGGAACTCAGACGCAGAAACTTACTCAAGAAGTTCAGGTATAAGAGAAGGATCAAAGAGTTAGAAGCTTCATTAAGATGGATGGTAGATGTGGATGTCCAAGTCAATCAATGGGTAGATATCAAAGAACTTATGGCCAAGATGTCCGAAATGAACACTAAACTTGATGAAATCACTCGTCAACCAACTGATTGTATTTGTTTCAAGAGCAATCATAGCACATCACAAAGTAGTAGTCAAAATATTGTCGAAGAAACAGACCGATCTTTAGAAGAAATTGTTGAATGTTCGAGTGATGGGTCTAAACCGAAGATCGATATCCACATTCATTGGAGTTCAAGAAAACGGAACAAAGACCGTGAGATCCGATTTGTCTTGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0043207 GO:0050896 GO:0051704 GO:0051707
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPW8

Protein Analysis

213

Amino Acids

24.69

Weight (kDa)

8.61

Isoelectric Point (pI)

64.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 5 - 143 6.9e-60 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014069)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50450 AT3G50460 AT3G50470 AT3G50480 AT3G50480
malus_domestica MD02G1163800.v1.1
prunus_persica Prupe.7G140100_v2.0.a1
rosa_laevigata RLG00000017290
rosa_multiflora Rmu_ssc0000257.1_g000011
rosa_roxburghii Rroxscaffold_2G00139110
rosa_rugosa Rorug02G0119300
rosa_samantha Rh2AG168800 Rh2BG176000 Rh2CG174400 Rh2DG174300 Rh6DG281400
rosa_wichuraiana Rw2G013210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 260
AclWI GGATC 3 cut(s) 146, 317, 616
AcoI YGGCCR 1 cut(s) 393
AfiI CCNNNNNNNGG 1 cut(s) 232
AflII CTTAAG 1 cut(s) 215
AgsI TTSAA 5 cut(s) 230, 463, 542, 597, 637
AluBI AGCT 3 cut(s) 32, 169, 326
AluI AGCT 3 cut(s) 32, 169, 326
AlwI GGATC 3 cut(s) 146, 317, 616
AoxI GGCC 1 cut(s) 393
ApeKI GCWGC 1 cut(s) 29
ArsI GACNNNNNNTTYG 2 cut(s) 478, 510
AspS9I GGNCC 1 cut(s) 112
AsuHPI GGTGA 2 cut(s) 163, 183
AvaII GGWCC 1 cut(s) 112
BalI TGGCCA 1 cut(s) 395
BbvI GCAGC 1 cut(s) 41
BccI CCATC 4 cut(s) 74, 330, 334, 548
BfrI CTTAAG 1 cut(s) 215
BglII AGATCT 2 cut(s) 86, 211
BisI GCNGC 1 cut(s) 30
BlpI GCTNAGC 1 cut(s) 170
BlsI GCNGC 1 cut(s) 31
Bme18I GGWCC 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 112
BmsI GCATC 5 cut(s) 52, 92, 112, 114, 229
BoxI GACNNNNGTC 1 cut(s) 158
Bpu1102I GCTNAGC 1 cut(s) 170
BpuEI CTTGAG 2 cut(s) 141, 269
Bsa29I ATCGAT 1 cut(s) 573
BsaBI GATNNNNATC 1 cut(s) 575
BsaJI CCNNGG 1 cut(s) 194
BsaXI ACNNNNNCTCC 2 cut(s) 30, 60
Bsc4I CCNNNNNNNGG 1 cut(s) 232
Bse118I RCCGGY 1 cut(s) 4
Bse8I GATNNNNATC 1 cut(s) 575
BseCI ATCGAT 1 cut(s) 573
BseDI CCNNGG 1 cut(s) 194
BseGI GGATG 2 cut(s) 345, 358
BseJI GATNNNNATC 1 cut(s) 575
BseLI CCNNNNNNNGG 1 cut(s) 232
BseMII CTCAG 1 cut(s) 280
BseXI GCAGC 1 cut(s) 41
BshFI GGCC 1 cut(s) 395
BshVI ATCGAT 1 cut(s) 573
BsiSI CCGG 1 cut(s) 5
BslI CCNNNNNNNGG 1 cut(s) 232
BsnI GGCC 1 cut(s) 395
Bsp143I GATC 7 cut(s) 86, 138, 211, 309, 521, 570, 621
Bsp1720I GCTNAGC 1 cut(s) 170
BspACI CCGC 1 cut(s) 260
BspANI GGCC 1 cut(s) 395
BspCNI CTCAG 1 cut(s) 279
BspDI ATCGAT 1 cut(s) 573
BspPI GGATC 3 cut(s) 146, 317, 616
BspTI CTTAAG 1 cut(s) 215
BsrFI RCCGGY 1 cut(s) 4
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 1 cut(s) 194
BssMI GATC 7 cut(s) 86, 138, 211, 309, 521, 570, 621
Bst4CI ACNGT 2 cut(s) 116, 617
BstAFI CTTAAG 1 cut(s) 215
BstDEI CTNAG 2 cut(s) 170, 266
BstENI CCTNNNNNAGG 1 cut(s) 230
BstF5I GGATG 2 cut(s) 345, 358
BstKTI GATC 7 cut(s) 89, 141, 214, 312, 524, 573, 624
BstMBI GATC 7 cut(s) 86, 138, 211, 309, 521, 570, 621
BstMWI GCNNNNNNNGC 2 cut(s) 29, 474
BstPAI GACNNNNGTC 1 cut(s) 158
BstV1I GCAGC 1 cut(s) 41
BstX2I RGATCY 3 cut(s) 86, 211, 621
BstYI RGATCY 3 cut(s) 86, 211, 621
Bsu15I ATCGAT 1 cut(s) 573
BsuRI GGCC 1 cut(s) 395
BsuTUI ATCGAT 1 cut(s) 573
BtsCI GGATG 2 cut(s) 345, 358
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 1 cut(s) 112
ClaI ATCGAT 1 cut(s) 573
CseI GACGC 1 cut(s) 279
CspCI CAANNNNNGTGG 2 cut(s) 569, 604
CviJI RGCY 6 cut(s) 32, 169, 236, 254, 326, 395
CviKI_1 RGCY 6 cut(s) 32, 169, 236, 254, 326, 395
DdeI CTNAG 2 cut(s) 170, 266
DpnI GATC 7 cut(s) 88, 140, 213, 311, 523, 572, 623
DpnII GATC 7 cut(s) 86, 138, 211, 309, 521, 570, 621
EaeI YGGCCR 1 cut(s) 393
Eco32I GATATC 2 cut(s) 379, 577
Eco47I GGWCC 1 cut(s) 112
EcoNI CCTNNNNNAGG 1 cut(s) 230
EcoRV GATATC 2 cut(s) 379, 577
EcoT22I ATGCAT 1 cut(s) 107
FaiI YATR 5 cut(s) 20, 258, 300, 392, 474
Fnu4HI GCNGC 1 cut(s) 30
FokI GGATG 2 cut(s) 352, 365
Fsp4HI GCNGC 1 cut(s) 30
GluI GCNGC 1 cut(s) 30
HaeIII GGCC 1 cut(s) 395
HapII CCGG 1 cut(s) 5
HgaI GACGC 1 cut(s) 279
HincII GTYRAC 1 cut(s) 440
HindII GTYRAC 1 cut(s) 440
HindIII AAGCTT 2 cut(s) 167, 324
HinfI GANTC 2 cut(s) 154, 188
HpaII CCGG 1 cut(s) 5
HphI GGTGA 2 cut(s) 163, 183
Hpy166II GTNNAC 1 cut(s) 440
Hpy188I TCNGA 3 cut(s) 269, 406, 626
Hpy188III TCNNGA 6 cut(s) 59, 119, 286, 463, 597, 634
Hpy8I GTNNAC 1 cut(s) 440
HpyAV CCTTC 2 cut(s) 65, 300
HpyCH4III ACNGT 2 cut(s) 116, 617
HpyCH4V TGCA 1 cut(s) 105
HpyF10VI GCNNNNNNNGC 2 cut(s) 29, 474
HpyF3I CTNAG 2 cut(s) 170, 266
Kzo9I GATC 7 cut(s) 86, 138, 211, 309, 521, 570, 621
LpnPI CCDG 3 cut(s) 9, 18, 280
Lsp1109I GCAGC 1 cut(s) 41
LweI GCATC 5 cut(s) 52, 92, 112, 114, 229
MaeIII GTNAC 1 cut(s) 151
MalI GATC 7 cut(s) 88, 140, 213, 311, 523, 572, 623
MboI GATC 7 cut(s) 86, 138, 211, 309, 521, 570, 621
MboII GAAGA 6 cut(s) 188, 197, 221, 521, 542, 580
MflI RGATCY 3 cut(s) 86, 211, 621
MlsI TGGCCA 1 cut(s) 395
MluCI AATT 1 cut(s) 534
MluNI TGGCCA 1 cut(s) 395
MlyI GAGTC 1 cut(s) 148
MnlI CCTC 2 cut(s) 189, 244
Mox20I TGGCCA 1 cut(s) 395
Mph1103I ATGCAT 1 cut(s) 107
MscI TGGCCA 1 cut(s) 395
MseI TTAA 2 cut(s) 216, 332
MslI CAYNNNNRTG 1 cut(s) 334
Msp20I TGGCCA 1 cut(s) 395
MspCI CTTAAG 1 cut(s) 215
MspI CCGG 1 cut(s) 5
MwoI GCNNNNNNNGC 2 cut(s) 29, 474
NdeII GATC 7 cut(s) 86, 138, 211, 309, 521, 570, 621
NmuCI GTSAC 1 cut(s) 151
NsiI ATGCAT 1 cut(s) 107
PfeI GAWTC 1 cut(s) 188
PkrI GCNGC 1 cut(s) 31
PleI GAGTC 1 cut(s) 148
PpsI GAGTC 1 cut(s) 148
PshAI GACNNNNGTC 1 cut(s) 158
PspPI GGNCC 1 cut(s) 112
PsuI RGATCY 3 cut(s) 86, 211, 621
RseI CAYNNNNRTG 1 cut(s) 334
SaqAI TTAA 2 cut(s) 216, 332
SatI GCNGC 1 cut(s) 30
Sau3AI GATC 7 cut(s) 86, 138, 211, 309, 521, 570, 621
Sau96I GGNCC 1 cut(s) 112
SchI GAGTC 1 cut(s) 148
SetI ASST 4 cut(s) 34, 171, 299, 328
SfaNI GCATC 5 cut(s) 52, 92, 112, 114, 229
SinI GGWCC 1 cut(s) 112
SmiMI CAYNNNNRTG 1 cut(s) 334
SmlI CTYRAG 3 cut(s) 156, 215, 284
SmoI CTYRAG 3 cut(s) 156, 215, 284
Sse9I AATT 1 cut(s) 534
SsiI CCGC 1 cut(s) 260
SspI AATATT 1 cut(s) 502
TaaI ACNGT 2 cut(s) 116, 617
TaqI TCGA 5 cut(s) 162, 207, 507, 548, 573
TaqII GACCGA 1 cut(s) 534
TasI AATT 1 cut(s) 534
TfiI GAWTC 1 cut(s) 188
Tru1I TTAA 2 cut(s) 216, 332
Tru9I TTAA 2 cut(s) 216, 332
TseFI GTSAC 1 cut(s) 151
TseI GCWGC 1 cut(s) 29
Tsp45I GTSAC 1 cut(s) 151
TspDTI ATGAA 4 cut(s) 318, 425, 441, 575
TspGWI ACGGA 2 cut(s) 135, 619
Vha464I CTTAAG 1 cut(s) 215
VpaK11BI GGWCC 1 cut(s) 112
XagI CCTNNNNNAGG 1 cut(s) 230
Zsp2I ATGCAT 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.