AT3G51730

Saposin (B) Domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0012842
Physical Location & Seq
Forward (+)
19186666 .. 19188733
2068 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G51730.1

Sequence Viewer

Length: 642 bp
ATGGGTCTTAAAGCTGGAACCTTTGTGCTTCTCTTGTTGGGTTTGATCTTGGTCTCTGACGCTAGATCTTTCGTTGACTCCACCATCTCAGAGAAAGTTTCCAACAAGGAAGACGTTTGCACTCTGTGTGAGGAATATGTTACTGATGCTCTCAGCTACCTTGAGAAAAATGTCACACAAGCGGAGATCATCGAAGATCTTCATGATCGGTGTTCCCAATTGCGCGGATATTCGCAGCAGTGCATAAGTTTGGTGGATTACTATGTTCCTCTTTTCTTCTTACAACTAGAGTCGTTTCAACCTCATTATTTCTGCAAGAGGATGAATCTTTGTGGCAAAGTTGTAGCTCTTGTCGAAGAAGCTCGTCAGGACAGTTGCGGTGTATGCCACAGGACTGTTTCAGAGATTCTCATCAAACTACAAGATCCTGACACACAGCTGGATATCGTTGAACTGCTTATCAAGGGATGCAAATCGCTCAAGAACTACGAGAAGAAGTGCAAGACGTTGGTGTTTGAATATGGACCTCTGATACTCGTAAATGCAGAGGAATTCCTAGTGAAAAACGACGTCTGCACACTCTTGCGCGCATGCCCCCCTGAGAAATCGGTTCTAAGGCAGCCGGAGTTGGCTGATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000323 GO:0001655 GO:0001664 GO:0001775 GO:0002252 GO:0002263 GO:0002274 GO:0002275 GO:0002283 GO:0002366 GO:0002376 GO:0002443 GO:0002444 GO:0002446 GO:0002576 GO:0003006 GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005102 GO:0005488 GO:0005515 GO:0005543 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005766 GO:0005773 GO:0005774 GO:0005775 GO:0005886 GO:0006629 GO:0006643 GO:0006664 GO:0006665 GO:0006687 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006887 GO:0006955 GO:0007154 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0007193 GO:0007275 GO:0008047 GO:0008150 GO:0008152 GO:0008289 GO:0009888 GO:0009894 GO:0009987 GO:0010506 GO:0010876 GO:0012505 GO:0012506 GO:0015711 GO:0015849 GO:0015925 GO:0016020 GO:0016192 GO:0016787 GO:0016798 GO:0019216 GO:0019222 GO:0022414 GO:0023052 GO:0030141 GO:0030154 GO:0030234 GO:0030659 GO:0030667 GO:0030850 GO:0030855 GO:0031090 GO:0031323 GO:0031329 GO:0031406 GO:0031410 GO:0031974 GO:0031982 GO:0032501 GO:0032502 GO:0032940 GO:0033036 GO:0033218 GO:0033293 GO:0035265 GO:0035577 GO:0035594 GO:0035627 GO:0036094 GO:0036230 GO:0040007 GO:0042119 GO:0042582 GO:0042802 GO:0042803 GO:0042886 GO:0043085 GO:0043167 GO:0043168 GO:0043177 GO:0043202 GO:0043208 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043299 GO:0043312 GO:0044093 GO:0044237 GO:0044238 GO:0044255 GO:0044421 GO:0044422 GO:0044424 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045055 GO:0045321 GO:0046625 GO:0046836 GO:0046903 GO:0046942 GO:0046983 GO:0048513 GO:0048589 GO:0048608 GO:0048731 GO:0048732 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0051861 GO:0060429 GO:0060736 GO:0060742 GO:0061458 GO:0065007 GO:0065009 GO:0070013 GO:0071702 GO:0071704 GO:0071705 GO:0071944 GO:0080090 GO:0097001 GO:0097367 GO:0097708 GO:0098588 GO:0098772 GO:0098805 GO:0098852 GO:0099503 GO:1901135 GO:1901264 GO:1901564 GO:1903509 GO:1905572 GO:1905573 GO:1905574 GO:1905575 GO:1905576 GO:1905577
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
SapB_1 SapB_2

Protein Analysis

213

Amino Acids

24.1

Weight (kDa)

5.0

Isoelectric Point (pI)

51.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SapB_1 PF05184 38 - 74 2.7e-11 Saposin-like type B, region 1
SapB_2 PF03489 79 - 111 2.4e-07 Saposin-like type B, region 2
SapB_1 PF05184 125 - 160 9.9e-07 Saposin-like type B, region 1
SapB_2 PF03489 165 - 198 2e-07 Saposin-like type B, region 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 573
AccII CGCG 2 cut(s) 225, 588
AciI CCGC 3 cut(s) 182, 225, 378
AclWI GGATC 1 cut(s) 419
AcsI RAATTY 1 cut(s) 551
AcyI GRCGYC 1 cut(s) 570
AdeI CACNNNGTG 1 cut(s) 126
AgsI TTSAA 3 cut(s) 299, 452, 518
AluBI AGCT 5 cut(s) 14, 156, 347, 362, 439
AluI AGCT 5 cut(s) 14, 156, 347, 362, 439
Alw26I GTCTC 1 cut(s) 58
AlwI GGATC 1 cut(s) 419
ApeKI GCWGC 2 cut(s) 235, 619
ApoI RAATTY 1 cut(s) 551
Asp700I GAANNNNTTC 1 cut(s) 198
AspLEI GCGC 3 cut(s) 225, 588, 590
AspS9I GGNCC 1 cut(s) 524
AvaII GGWCC 1 cut(s) 524
BbsI GAAGAC 1 cut(s) 117
BbvI GCAGC 2 cut(s) 247, 631
BccI CCATC 1 cut(s) 92
BcoDI GTCTC 1 cut(s) 58
BfaI CTAG 3 cut(s) 63, 287, 557
BglII AGATCT 2 cut(s) 65, 196
BisI GCNGC 2 cut(s) 236, 620
BlsI GCNGC 2 cut(s) 237, 621
Bme18I GGWCC 1 cut(s) 524
BmgT120I GGNCC 1 cut(s) 524
BmiI GGNNCC 1 cut(s) 19
BmsI GCATC 2 cut(s) 136, 458
BpiI GAAGAC 1 cut(s) 117
BpuEI CTTGAG 2 cut(s) 182, 464
BsaBI GATNNNNATC 2 cut(s) 410, 472
BsaHI GRCGYC 1 cut(s) 570
BsaI GGTCTC 1 cut(s) 58
Bse8I GATNNNNATC 2 cut(s) 410, 472
BseGI GGATG 2 cut(s) 327, 473
BseJI GATNNNNATC 2 cut(s) 410, 472
BseMII CTCAG 3 cut(s) 102, 166, 591
BsePI GCGCGC 1 cut(s) 586
BseXI GCAGC 2 cut(s) 247, 631
BsgI GTGCAG 1 cut(s) 559
Bsh1236I CGCG 2 cut(s) 225, 588
BsiSI CCGG 1 cut(s) 623
BsmAI GTCTC 1 cut(s) 58
Bso31I GGTCTC 1 cut(s) 58
Bsp143I GATC 6 cut(s) 45, 65, 186, 196, 205, 424
BspACI CCGC 3 cut(s) 182, 225, 378
BspCNI CTCAG 3 cut(s) 101, 165, 592
BspFNI CGCG 2 cut(s) 225, 588
BspHI TCATGA 1 cut(s) 202
BspLI GGNNCC 1 cut(s) 19
BspPI GGATC 1 cut(s) 419
BspTNI GGTCTC 1 cut(s) 58
BssHII GCGCGC 1 cut(s) 586
BssMI GATC 6 cut(s) 45, 65, 186, 196, 205, 424
BssNI GRCGYC 1 cut(s) 570
Bst4CI ACNGT 2 cut(s) 374, 397
BstACI GRCGYC 1 cut(s) 570
BstC8I GCNNGC 2 cut(s) 588, 592
BstDEI CTNAG 4 cut(s) 88, 152, 600, 614
BstF5I GGATG 2 cut(s) 327, 473
BstFNI CGCG 2 cut(s) 225, 588
BstHHI GCGC 3 cut(s) 225, 588, 590
BstKTI GATC 6 cut(s) 48, 68, 189, 199, 208, 427
BstMAI GTCTC 1 cut(s) 58
BstMBI GATC 6 cut(s) 45, 65, 186, 196, 205, 424
BstMWI GCNNNNNNNGC 1 cut(s) 384
BstNSI RCATGY 1 cut(s) 594
BstUI CGCG 2 cut(s) 225, 588
BstV1I GCAGC 2 cut(s) 247, 631
BstV2I GAAGAC 1 cut(s) 117
BstX2I RGATCY 3 cut(s) 65, 196, 424
BstYI RGATCY 3 cut(s) 65, 196, 424
BtsCI GGATG 2 cut(s) 327, 473
BtsI GCAGTG 1 cut(s) 245
BtsIMutI CAGTG 1 cut(s) 245
Cac8I GCNNGC 2 cut(s) 588, 592
CciI TCATGA 1 cut(s) 202
CfoI GCGC 3 cut(s) 225, 588, 590
Cfr13I GGNCC 1 cut(s) 524
CseI GACGC 1 cut(s) 68
CviAII CATG 2 cut(s) 203, 591
CviJI RGCY 7 cut(s) 14, 156, 347, 362, 439, 622, 632
CviKI_1 RGCY 7 cut(s) 14, 156, 347, 362, 439, 622, 632
DdeI CTNAG 4 cut(s) 88, 152, 600, 614
DpnI GATC 6 cut(s) 47, 67, 188, 198, 207, 426
DpnII GATC 6 cut(s) 45, 65, 186, 196, 205, 424
DraIII CACNNNGTG 1 cut(s) 126
Eco31I GGTCTC 1 cut(s) 58
Eco32I GATATC 1 cut(s) 445
Eco47I GGWCC 1 cut(s) 524
EcoRI GAATTC 1 cut(s) 551
EcoRV GATATC 1 cut(s) 445
FaeI CATG 2 cut(s) 206, 594
FaiI YATR 7 cut(s) 138, 204, 245, 264, 385, 522, 592
FatI CATG 2 cut(s) 202, 590
Fnu4HI GCNGC 2 cut(s) 236, 620
FokI GGATG 2 cut(s) 334, 480
Fsp4HI GCNGC 2 cut(s) 236, 620
FspBI CTAG 3 cut(s) 63, 287, 557
GlaI GCGC 3 cut(s) 224, 587, 589
GluI GCNGC 2 cut(s) 236, 620
HapII CCGG 1 cut(s) 623
HgaI GACGC 1 cut(s) 68
HhaI GCGC 3 cut(s) 225, 588, 590
Hin1I GRCGYC 1 cut(s) 570
Hin1II CATG 2 cut(s) 206, 594
Hin6I GCGC 3 cut(s) 223, 586, 588
HinP1I GCGC 3 cut(s) 223, 586, 588
HincII GTYRAC 1 cut(s) 76
HindII GTYRAC 1 cut(s) 76
HinfI GANTC 5 cut(s) 77, 290, 325, 406, 635
HpaII CCGG 1 cut(s) 623
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 4 cut(s) 58, 91, 403, 531
Hpy188III TCNNGA 5 cut(s) 203, 368, 428, 481, 639
Hpy8I GTNNAC 1 cut(s) 76
Hpy99I CGWCG 1 cut(s) 572
HpyCH4III ACNGT 2 cut(s) 374, 397
HpyCH4IV ACGT 3 cut(s) 114, 506, 570
HpyCH4V TGCA 7 cut(s) 120, 243, 315, 471, 501, 545, 576
HpyF10VI GCNNNNNNNGC 1 cut(s) 384
HpyF3I CTNAG 4 cut(s) 88, 152, 600, 614
HpySE526I ACGT 3 cut(s) 114, 506, 570
Hsp92I GRCGYC 1 cut(s) 570
Hsp92II CATG 2 cut(s) 206, 594
HspAI GCGC 3 cut(s) 223, 586, 588
Kzo9I GATC 6 cut(s) 45, 65, 186, 196, 205, 424
LpnPI CCDG 6 cut(s) 353, 376, 425, 441, 612, 636
Lsp1109I GCAGC 2 cut(s) 247, 631
LweI GCATC 2 cut(s) 136, 458
MaeI CTAG 3 cut(s) 63, 287, 557
MaeII ACGT 3 cut(s) 114, 506, 570
MaeIII GTNAC 2 cut(s) 139, 172
MalI GATC 6 cut(s) 47, 67, 188, 198, 207, 426
MboI GATC 6 cut(s) 45, 65, 186, 196, 205, 424
MboII GAAGA 6 cut(s) 122, 191, 206, 268, 368, 505
MfeI CAATTG 1 cut(s) 218
MflI RGATCY 3 cut(s) 65, 196, 424
MluCI AATT 2 cut(s) 218, 551
MlyI GAGTC 2 cut(s) 71, 299
MmeI TCCRAC 1 cut(s) 126
MnlI CCTC 6 cut(s) 124, 279, 312, 312, 537, 541
MroXI GAANNNNTTC 1 cut(s) 198
MseI TTAA 1 cut(s) 9
MspA1I CMGCKG 1 cut(s) 439
MspI CCGG 1 cut(s) 623
MunI CAATTG 1 cut(s) 218
MvnI CGCG 2 cut(s) 225, 588
MwoI GCNNNNNNNGC 1 cut(s) 384
NdeII GATC 6 cut(s) 45, 65, 186, 196, 205, 424
NlaIII CATG 2 cut(s) 206, 594
NlaIV GGNNCC 1 cut(s) 19
NmuCI GTSAC 1 cut(s) 172
NspI RCATGY 1 cut(s) 594
PaeI GCATGC 1 cut(s) 594
PagI TCATGA 1 cut(s) 202
PauI GCGCGC 1 cut(s) 586
PdmI GAANNNNTTC 1 cut(s) 198
PfeI GAWTC 3 cut(s) 325, 406, 635
PkrI GCNGC 2 cut(s) 237, 621
PleI GAGTC 2 cut(s) 71, 298
PpsI GAGTC 2 cut(s) 71, 298
PspN4I GGNNCC 1 cut(s) 19
PspPI GGNCC 1 cut(s) 524
PsuI RGATCY 3 cut(s) 65, 196, 424
PteI GCGCGC 1 cut(s) 586
PvuII CAGCTG 1 cut(s) 439
SaqAI TTAA 1 cut(s) 9
SatI GCNGC 2 cut(s) 236, 620
Sau3AI GATC 6 cut(s) 45, 65, 186, 196, 205, 424
Sau96I GGNCC 1 cut(s) 524
SchI GAGTC 2 cut(s) 71, 299
SfaNI GCATC 2 cut(s) 136, 458
SinI GGWCC 1 cut(s) 524
SmlI CTYRAG 2 cut(s) 161, 479
SmoI CTYRAG 2 cut(s) 161, 479
SphI GCATGC 1 cut(s) 594
Sse9I AATT 2 cut(s) 218, 551
SsiI CCGC 3 cut(s) 182, 225, 378
SspMI CTAG 3 cut(s) 63, 287, 557
TaaI ACNGT 2 cut(s) 374, 397
TaiI ACGT 3 cut(s) 117, 509, 573
TaqI TCGA 2 cut(s) 192, 354
TasI AATT 2 cut(s) 218, 551
TfiI GAWTC 3 cut(s) 325, 406, 635
Tru1I TTAA 1 cut(s) 9
Tru9I TTAA 1 cut(s) 9
TscAI CASTG 1 cut(s) 245
TseFI GTSAC 1 cut(s) 172
TseI GCWGC 2 cut(s) 235, 619
Tsp45I GTSAC 1 cut(s) 172
TspDTI ATGAA 2 cut(s) 191, 338
TspRI CASTG 1 cut(s) 245
VpaK11BI GGWCC 1 cut(s) 524
XapI RAATTY 1 cut(s) 551
XceI RCATGY 1 cut(s) 594
XmnI GAANNNNTTC 1 cut(s) 198
XspI CTAG 3 cut(s) 63, 287, 557
ZraI GACGTC 1 cut(s) 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.