Rorug03G0047200

Saposin (B) Domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0012842
Physical Location & Seq
Forward (+)
3622530 .. 3627739
5210 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0047200.1

Sequence Viewer

Length: 1833 bp
ATGTCGTCTCCTGCAGAAGCATCGAACAAACCTGATTCGACTGAAAAGAAAGCCGTGTCCCCTGCAGAAGCATCAAATACTAGAAAGAAAACCCCAAAATCATTGAAGGGCAAAGCTAAAATCGTGAAGAAATCTCTGATTGATAACAGTTTGAAAGGTAGCAAAGCTACAAGTAGTTCACTGGCTAATGCGAACAGGAGGAGGTTCAGGAATAAGGGTCCCAAAGAGAGCAGCAACAAAAGAGAAGATGTGAAGAAGGTGAGTTCAAGAAAAGATAAGCAACAGAAAAATACCGATGAACAGCAGACAGATAAGAGCCATCAGGCACAAAAGAATAAAGGAAAGGTGGACGAAGTCGAAAAGAGCCAGCAAAAGAATACAGAAAAACGTCATGGGCCGGACAAGAGTGTAAAGAGTGAAATGAATGGCAAAAAACGTGATGAAAAGAGAGAAAAGCTTGGTGGTTTAATATTCATGTGCAGTGGAAAGACAAAGCCAGACTGCTTCCATTATCGTATAATGGGTGTTTCAGTGGGTAAAAAAGACACTGTGTTGGGTATTAAACCTGGGTTGAAGCTTTTTCTCTATGATTTTGACCTTAAGCTTTTATACGGAGTTTACAAGGCATCCTCTTCAGGTGGCTTAAAACTCGAGCCAAAAGCTTTCGGTGGGGCCTTCCCTGCTCAGGTTCGGTTCAATGTGGAGAAAGATTGCCTTCCTCTGCATGAAAGAGTTTTCAGGAAGGCAATAAAGGAAAACTATGATGAGAAGAACAAGTTCAAAACAGAACTTACAGTTCGACAAGTCAGGAAGCTCACAGCACTTTTCCGACCGGCTCAAGTTCATTTATCTTCTCTACCCACACGTTCCCCACTCAGGGCAGACAATCGAGATAGGGAAACTCGTGGGAGGGAGAGAGAAGTGCTGCCACTTTCACATAGGGATCCACATGCTAGAGATCCCTATCCCAATGGCAATGCCAGGAGTTATCCAGCGTTAGCTCATGAAAGGGAGCAGCATGCAAGGAGAGAGGAAATTCCTCGTGATTTGTACCTCTCTGAAAAGGAATATCGAGCTTATGGTCTTCAAGGAGAGAGAAGAAATCTGGCTCCTCGCCCTGTTAGTCCAGCTGTGGAAGTTCAGCACAGAGATTATGAAAGGGATCATTTTGTAAGACAACCAAACCTTTATAGGGAGGCTGCCTCTGTACATCGAGAAACTGTTCCTTCTGATCCTCTCTACTTGAATCAGAAAGAGTACCCAGCTTATACTGTTGGTGCTAGACATGAGTTGCCTCCTGCAACAGCTGCTGCCGCTGTAGATTTGTATGCAAGGGATCCTTACTATAGTAACTATAGCTATTATGGGGCTTCATCATTGGACCAATATACAGCCCCTCAGAGGAGAGAGGAGGTTCCTTTGGGATCTTACTTAGCTGGTGCAAGGAGAGAATCTTACCTGGTTGAGAATGATCCCATGCAAAGGAGAGCACCTGATCTTGTAGAGAGAAGTTTGTACTCCGCACATGCTGCTGCTGCCATTGATGATCGTTTAGAGAGAAGCTTATATTCAGGACGTGGTGTAGGCGATGATCGTTTAGAGAGAAGCTTATACTCGGCACGTGCTGCAGGCGATGATCGTTTAGAGAGAAGCTTATACTCGGCACATGCTGCTACTGCTGGTGATGATCGTTTAGGGAGAAGCTTATACTCGGCACGTGCTGCTACTGCTCTGGATGCTGTTGCTCCATACCAGGTGACGAAGCCTGAAGCTCTGCGTACGCCAGTTTCATCTCGCTATGCATTTGCTGGTGCATCATACTCCTATCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000323 GO:0001655 GO:0001664 GO:0001775 GO:0002252 GO:0002263 GO:0002274 GO:0002275 GO:0002283 GO:0002366 GO:0002376 GO:0002443 GO:0002444 GO:0002446 GO:0002576 GO:0003006 GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005102 GO:0005488 GO:0005515 GO:0005543 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005766 GO:0005773 GO:0005774 GO:0005775 GO:0005886 GO:0006629 GO:0006643 GO:0006664 GO:0006665 GO:0006687 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006887 GO:0006955 GO:0007154 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0007193 GO:0007275 GO:0008047 GO:0008150 GO:0008152 GO:0008289 GO:0009888 GO:0009894 GO:0009987 GO:0010506 GO:0010876 GO:0012505 GO:0012506 GO:0015711 GO:0015849 GO:0015925 GO:0016020 GO:0016192 GO:0016787 GO:0016798 GO:0019216 GO:0019222 GO:0022414 GO:0023052 GO:0030141 GO:0030154 GO:0030234 GO:0030659 GO:0030667 GO:0030850 GO:0030855 GO:0031090 GO:0031323 GO:0031329 GO:0031406 GO:0031410 GO:0031974 GO:0031982 GO:0032501 GO:0032502 GO:0032940 GO:0033036 GO:0033218 GO:0033293 GO:0035265 GO:0035577 GO:0035594 GO:0035627 GO:0036094 GO:0036230 GO:0040007 GO:0042119 GO:0042582 GO:0042802 GO:0042803 GO:0042886 GO:0043085 GO:0043167 GO:0043168 GO:0043177 GO:0043202 GO:0043208 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043299 GO:0043312 GO:0044093 GO:0044237 GO:0044238 GO:0044255 GO:0044421 GO:0044422 GO:0044424 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045055 GO:0045321 GO:0046625 GO:0046836 GO:0046903 GO:0046942 GO:0046983 GO:0048513 GO:0048589 GO:0048608 GO:0048731 GO:0048732 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0051861 GO:0060429 GO:0060736 GO:0060742 GO:0061458 GO:0065007 GO:0065009 GO:0070013 GO:0071702 GO:0071704 GO:0071705 GO:0071944 GO:0080090 GO:0097001 GO:0097367 GO:0097708 GO:0098588 GO:0098772 GO:0098805 GO:0098852 GO:0099503 GO:1901135 GO:1901264 GO:1901564 GO:1903509 GO:1905572 GO:1905573 GO:1905574 GO:1905575 GO:1905576 GO:1905577
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
SapB_1 SapB_2

Protein Analysis

610

Amino Acids

68.9

Weight (kDa)

9.79

Isoelectric Point (pI)

41.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dev_Cell_Death PF10539 154 - 276 1.2e-48 Development and cell death domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 1314, 1521
AclWI GGATC 9 cut(s) 938, 951, 953, 1170, 1226, 1331, 1344, 1432, 1466
AcsI RAATTY 1 cut(s) 1035
AcuI CTGAAG 2 cut(s) 618, 1788
AcvI CACGTG 2 cut(s) 1622, 1718
AfaI GTAC 5 cut(s) 1052, 1209, 1259, 1517, 1780
AfiI CCNNNNNNNGG 6 cut(s) 685, 876, 877, 1192, 1401, 1482
AflII CTTAAG 1 cut(s) 599
AflIII ACRYGT 1 cut(s) 863
AgsI TTSAA 8 cut(s) 106, 154, 267, 574, 697, 781, 1088, 1246
AjiI CACGTC 1 cut(s) 1577
AjnI CCWGG 4 cut(s) 565, 980, 1458, 1752
Alw21I GWGCWC 1 cut(s) 1492
Alw26I GTCTC 1 cut(s) 12
AlwI GGATC 9 cut(s) 938, 951, 953, 1170, 1226, 1331, 1344, 1432, 1466
AlwNI CAGNNNCTG 1 cut(s) 1310
Ama87I CYCGRG 1 cut(s) 650
AoxI GGCC 2 cut(s) 395, 672
ApoI RAATTY 1 cut(s) 1035
Asp700I GAANNNNTTC 2 cut(s) 776, 1221
AspS9I GGNCC 4 cut(s) 218, 395, 672, 1381
AsuHPI GGTGA 3 cut(s) 271, 1694, 1768
AvaI CYCGRG 1 cut(s) 650
AvaII GGWCC 2 cut(s) 218, 1381
BamHI GGATCC 2 cut(s) 943, 1336
BauI CACGAG 2 cut(s) 903, 1041
BbrPI CACGTG 2 cut(s) 1622, 1718
BbsI GAAGAC 1 cut(s) 1076
Bbv12I GWGCWC 1 cut(s) 1492
BccI CCATC 1 cut(s) 327
BceAI ACGGC 1 cut(s) 38
BcgI CGANNNNNNTGC 1 cut(s) 37
BciT130I CCWGG 4 cut(s) 567, 982, 1460, 1754
BcoDI GTCTC 1 cut(s) 12
BfaI CTAG 3 cut(s) 81, 954, 1281
BfmI CTRYAG 6 cut(s) 12, 63, 1317, 1345, 1354, 1626
BfrI CTTAAG 1 cut(s) 599
Bme1390I CCNGG 4 cut(s) 567, 982, 1460, 1754
Bme18I GGWCC 2 cut(s) 218, 1381
BmeT110I CYCGRG 1 cut(s) 650
BmgBI CACGTC 1 cut(s) 1577
BmgT120I GGNCC 4 cut(s) 218, 395, 672, 1381
BmiI GGNNCC 7 cut(s) 219, 220, 673, 945, 1110, 1338, 1416
BmrFI CCNGG 4 cut(s) 567, 982, 1460, 1754
BmsI GCATC 5 cut(s) 29, 80, 635, 1726, 1823
BpiI GAAGAC 1 cut(s) 1076
BplI GAGNNNNNCTC 2 cut(s) 1187, 1219
Bpu10I CCTNAGC 1 cut(s) 684
BpuEI CTTGAG 1 cut(s) 822
BsaAI YACGTR 2 cut(s) 1622, 1718
BsaBI GATNNNNATC 1 cut(s) 963
BsaJI CCNNGG 1 cut(s) 566
Bsc4I CCNNNNNNNGG 6 cut(s) 685, 876, 877, 1192, 1401, 1482
Bse118I RCCGGY 1 cut(s) 832
Bse1I ACTGG 2 cut(s) 186, 1784
Bse3DI GCAATG 1 cut(s) 982
Bse8I GATNNNNATC 1 cut(s) 963
BseBI CCWGG 4 cut(s) 567, 982, 1460, 1754
BseDI CCNNGG 1 cut(s) 566
BseGI GGATG 2 cut(s) 626, 1741
BseJI GATNNNNATC 1 cut(s) 963
BseLI CCNNNNNNNGG 6 cut(s) 685, 876, 877, 1192, 1401, 1482
BseMI GCAATG 1 cut(s) 982
BseMII CTCAG 3 cut(s) 698, 889, 1412
BseNI ACTGG 2 cut(s) 186, 1784
BseRI GAGGAG 4 cut(s) 214, 1101, 1417, 1424
BseYI CCCAGC 1 cut(s) 1261
BsgI GTGCAG 1 cut(s) 499
Bsh1285I CGRYCG 1 cut(s) 833
BshFI GGCC 2 cut(s) 397, 674
BsiEI CGRYCG 1 cut(s) 833
BsiHKAI GWGCWC 1 cut(s) 1492
BsiHKCI CYCGRG 1 cut(s) 650
BsiSI CCGG 2 cut(s) 398, 833
BsiWI CGTACG 1 cut(s) 1778
BslFI GGGAC 2 cut(s) 43, 204
BslI CCNNNNNNNGG 6 cut(s) 685, 876, 877, 1192, 1401, 1482
BsmAI GTCTC 1 cut(s) 12
BsmBI CGTCTC 1 cut(s) 12
BsmFI GGGAC 2 cut(s) 43, 204
BsnI GGCC 2 cut(s) 397, 674
BsoBI CYCGRG 1 cut(s) 650
Bsp1286I GDGCHC 1 cut(s) 1492
Bsp1407I TGTACA 1 cut(s) 1207
BspACI CCGC 2 cut(s) 1314, 1521
BspANI GGCC 2 cut(s) 397, 674
BspCNI CTCAG 3 cut(s) 697, 888, 1411
BspHI TCATGA 1 cut(s) 1003
BspLI GGNNCC 7 cut(s) 219, 220, 673, 945, 1110, 1338, 1416
BspMAI CTGCAG 3 cut(s) 16, 67, 1630
BspPI GGATC 9 cut(s) 938, 951, 953, 1170, 1226, 1331, 1344, 1432, 1466
BspTI CTTAAG 1 cut(s) 599
BsrDI GCAATG 1 cut(s) 982
BsrFI RCCGGY 1 cut(s) 832
BsrGI TGTACA 1 cut(s) 1207
BsrI ACTGG 2 cut(s) 186, 1784
BssAI RCCGGY 1 cut(s) 832
BssECI CCNNGG 1 cut(s) 566
BssSI CACGAG 2 cut(s) 903, 1041
Bst2BI CACGAG 2 cut(s) 903, 1041
Bst2UI CCWGG 4 cut(s) 567, 982, 1460, 1754
Bst4CI ACNGT 5 cut(s) 149, 550, 796, 1222, 1273
Bst6I CTCTTC 1 cut(s) 637
BstAFI CTTAAG 1 cut(s) 599
BstAPI GCANNNNNTGC 5 cut(s) 1307, 1529, 1625, 1670, 1721
BstAUI TGTACA 1 cut(s) 1207
BstBAI YACGTR 2 cut(s) 1622, 1718
BstC8I GCNNGC 3 cut(s) 368, 1020, 1630
BstDEI CTNAG 4 cut(s) 684, 875, 1398, 1432
BstF5I GGATG 2 cut(s) 626, 1741
BstMAI GTCTC 1 cut(s) 12
BstMCI CGRYCG 1 cut(s) 833
BstNI CCWGG 4 cut(s) 567, 982, 1460, 1754
BstNSI RCATGY 4 cut(s) 953, 1022, 1529, 1670
BstSCI CCNGG 4 cut(s) 565, 980, 1458, 1752
BstSFI CTRYAG 6 cut(s) 12, 63, 1317, 1345, 1354, 1626
BstV2I GAAGAC 1 cut(s) 1076
BstX2I RGATCY 4 cut(s) 943, 958, 1336, 1424
BstYI RGATCY 4 cut(s) 943, 958, 1336, 1424
BsuRI GGCC 2 cut(s) 397, 674
BtgZI GCGATG 2 cut(s) 1602, 1647
BtrI CACGTC 1 cut(s) 1577
BtsCI GGATG 2 cut(s) 626, 1741
BtsI GCAGTG 1 cut(s) 487
BtsIMutI CAGTG 4 cut(s) 179, 487, 537, 546
Cac8I GCNNGC 3 cut(s) 368, 1020, 1630
CaiI CAGNNNCTG 1 cut(s) 1310
CciI TCATGA 1 cut(s) 1003
Cfr10I RCCGGY 1 cut(s) 832
Cfr13I GGNCC 4 cut(s) 218, 395, 672, 1381
CsiI ACCWGGT 2 cut(s) 1458, 1752
Csp6I GTAC 5 cut(s) 1051, 1208, 1258, 1516, 1779
CviQI GTAC 5 cut(s) 1051, 1208, 1258, 1516, 1779
DdeI CTNAG 4 cut(s) 684, 875, 1398, 1432
Eam1104I CTCTTC 1 cut(s) 637
EarI CTCTTC 1 cut(s) 637
Eco47I GGWCC 2 cut(s) 218, 1381
Eco57I CTGAAG 2 cut(s) 618, 1788
Eco72I CACGTG 2 cut(s) 1622, 1718
Eco88I CYCGRG 1 cut(s) 650
EcoO109I RGGNCCY 2 cut(s) 218, 672
EcoRII CCWGG 4 cut(s) 565, 980, 1458, 1752
EcoT22I ATGCAT 1 cut(s) 1804
Esp3I CGTCTC 1 cut(s) 12
FalI AAGNNNNNCTT 4 cut(s) 699, 731, 1324, 1356
FaqI GGGAC 2 cut(s) 43, 204
FokI GGATG 2 cut(s) 613, 1748
FspBI CTAG 3 cut(s) 81, 954, 1281
GsaI CCCAGC 1 cut(s) 1265
HaeIII GGCC 2 cut(s) 397, 674
HapII CCGG 2 cut(s) 398, 833
HindIII AAGCTT 8 cut(s) 455, 575, 602, 660, 1561, 1606, 1651, 1702
HinfI GANTC 3 cut(s) 35, 1246, 1451
HpaII CCGG 2 cut(s) 398, 833
HphI GGTGA 3 cut(s) 271, 1694, 1768
Hpy166II GTNNAC 3 cut(s) 179, 349, 619
Hpy188I TCNGA 6 cut(s) 138, 830, 1060, 1231, 1251, 1401
Hpy8I GTNNAC 3 cut(s) 179, 349, 619
HpyAV CCTTC 6 cut(s) 100, 250, 685, 725, 736, 1236
HpyCH4III ACNGT 5 cut(s) 149, 550, 796, 1222, 1273
HpyCH4IV ACGT 6 cut(s) 388, 436, 865, 1576, 1621, 1717
HpyF3I CTNAG 4 cut(s) 684, 875, 1398, 1432
HpySE526I ACGT 6 cut(s) 388, 436, 865, 1576, 1621, 1717
KflI GGGWCCC 1 cut(s) 218
LmnI GCTCC 3 cut(s) 1012, 1114, 1750
LweI GCATC 5 cut(s) 29, 80, 635, 1726, 1823
MabI ACCWGGT 2 cut(s) 1458, 1752
MaeI CTAG 3 cut(s) 81, 954, 1281
MaeII ACGT 6 cut(s) 388, 436, 865, 1576, 1621, 1717
MaeIII GTNAC 2 cut(s) 1349, 1756
MboII GAAGA 8 cut(s) 139, 257, 265, 624, 781, 843, 1076, 1110
MflI RGATCY 4 cut(s) 943, 958, 1336, 1424
MhlI GDGCHC 1 cut(s) 1492
MluCI AATT 1 cut(s) 1035
MmeI TCCRAC 1 cut(s) 853
Mph1103I ATGCAT 1 cut(s) 1804
MroXI GAANNNNTTC 2 cut(s) 776, 1221
MseI TTAA 4 cut(s) 467, 561, 600, 644
MspA1I CMGCKG 3 cut(s) 1130, 1307, 1316
MspCI CTTAAG 1 cut(s) 599
MspI CCGG 2 cut(s) 398, 833
MspR9I CCNGG 4 cut(s) 567, 982, 1460, 1754
MvaI CCWGG 4 cut(s) 567, 982, 1460, 1754
NlaIV GGNNCC 7 cut(s) 219, 220, 673, 945, 1110, 1338, 1416
NmeAIII GCCGAG 3 cut(s) 1595, 1640, 1691
NmuCI GTSAC 1 cut(s) 1756
NsiI ATGCAT 1 cut(s) 1804
NspI RCATGY 4 cut(s) 953, 1022, 1529, 1670
PaeI GCATGC 1 cut(s) 1022
PaeR7I CTCGAG 1 cut(s) 650
PagI TCATGA 1 cut(s) 1003
PdmI GAANNNNTTC 2 cut(s) 776, 1221
PfeI GAWTC 3 cut(s) 35, 1246, 1451
Pfl23II CGTACG 1 cut(s) 1778
PflFI GACNNNGTC 1 cut(s) 353
PmaCI CACGTG 2 cut(s) 1622, 1718
PmlI CACGTG 2 cut(s) 1622, 1718
Ppu21I YACGTR 2 cut(s) 1622, 1718
PpuMI RGGWCCY 1 cut(s) 218
Psp5II RGGWCCY 1 cut(s) 218
Psp6I CCWGG 4 cut(s) 565, 980, 1458, 1752
PspCI CACGTG 2 cut(s) 1622, 1718
PspFI CCCAGC 1 cut(s) 1261
PspGI CCWGG 4 cut(s) 565, 980, 1458, 1752
PspLI CGTACG 1 cut(s) 1778
PspN4I GGNNCC 7 cut(s) 219, 220, 673, 945, 1110, 1338, 1416
PspPI GGNCC 4 cut(s) 218, 395, 672, 1381
PspPPI RGGWCCY 1 cut(s) 218
PspXI VCTCGAGB 1 cut(s) 650
PstI CTGCAG 3 cut(s) 16, 67, 1630
PstNI CAGNNNCTG 1 cut(s) 1310
PsuI RGATCY 4 cut(s) 943, 958, 1336, 1424
PsyI GACNNNGTC 1 cut(s) 353
PvuII CAGCTG 2 cut(s) 1130, 1307
RsaI GTAC 5 cut(s) 1052, 1209, 1259, 1517, 1780
RsaNI GTAC 5 cut(s) 1051, 1208, 1258, 1516, 1779
SaqAI TTAA 4 cut(s) 467, 561, 600, 644
Sau96I GGNCC 4 cut(s) 218, 395, 672, 1381
ScrFI CCNGG 4 cut(s) 567, 982, 1460, 1754
SduI GDGCHC 1 cut(s) 1492
SexAI ACCWGGT 2 cut(s) 1458, 1752
SfaNI GCATC 5 cut(s) 29, 80, 635, 1726, 1823
SfcI CTRYAG 6 cut(s) 12, 63, 1317, 1345, 1354, 1626
Sfr274I CTCGAG 1 cut(s) 650
SinI GGWCC 2 cut(s) 218, 1381
SlaI CTCGAG 1 cut(s) 650
SmlI CTYRAG 3 cut(s) 599, 650, 837
SmoI CTYRAG 3 cut(s) 599, 650, 837
SphI GCATGC 1 cut(s) 1022
Sse9I AATT 1 cut(s) 1035
SsiI CCGC 2 cut(s) 1314, 1521
SspI AATATT 1 cut(s) 471
SspMI CTAG 3 cut(s) 81, 954, 1281
StyD4I CCNGG 4 cut(s) 565, 980, 1458, 1752
TaaI ACNGT 5 cut(s) 149, 550, 796, 1222, 1273
TaiI ACGT 6 cut(s) 391, 439, 868, 1579, 1624, 1720
TaqI TCGA 8 cut(s) 23, 38, 357, 651, 799, 889, 1072, 1213
TasI AATT 1 cut(s) 1035
TatI WGTACW 2 cut(s) 1207, 1515
TauI GCSGC 1 cut(s) 1316
TfiI GAWTC 3 cut(s) 35, 1246, 1451
Tru1I TTAA 4 cut(s) 467, 561, 600, 644
Tru9I TTAA 4 cut(s) 467, 561, 600, 644
TscAI CASTG 4 cut(s) 186, 487, 537, 553
TseFI GTSAC 1 cut(s) 1756
Tsp45I GTSAC 1 cut(s) 1756
TspGWI ACGGA 1 cut(s) 627
TspRI CASTG 4 cut(s) 186, 487, 537, 553
Tth111I GACNNNGTC 1 cut(s) 353
Vha464I CTTAAG 1 cut(s) 599
VpaK11BI GGWCC 2 cut(s) 218, 1381
XapI RAATTY 1 cut(s) 1035
XceI RCATGY 4 cut(s) 953, 1022, 1529, 1670
XhoI CTCGAG 1 cut(s) 650
XmnI GAANNNNTTC 2 cut(s) 776, 1221
XspI CTAG 3 cut(s) 81, 954, 1281
Zsp2I ATGCAT 1 cut(s) 1804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.