MD04G1171500.v1.1

Saposin (B) Domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0012842
Physical Location & Seq
Forward (+)
26315802 .. 26317742
1941 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1171500.v1.1.491

Sequence Viewer

Length: 342 bp
ATGGTTAAAAACATCAGCACCATGGACGTCAGAGTTGGGGTTTTGTTCCTTTTTGTGTTGGCAGCGAGTTGGGCTTGTGATGCTAGACAACTGACAAACCTTGATTTGTCTGTTTCAAAGTCAGGCCACCTGGTGGAAACTCAAACCTTCCAAGCTGAAGAAGTTGTTGGGAATGAGAAAGCGTGCACATTGTGTGAGGAGTTTGCTGGCCAAGCGCTTGATTACATTGGTGAAAACAAGACCCAGACAGAGATCGTCGAATACCTACATCAGACCTGCTCTCAGTTGCGCTCTTTCAAGCAGCAGGTAGTCACCCCTTCTAAATTTCTACAAGATTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000323 GO:0001655 GO:0001664 GO:0001775 GO:0002252 GO:0002263 GO:0002274 GO:0002275 GO:0002283 GO:0002366 GO:0002376 GO:0002443 GO:0002444 GO:0002446 GO:0002576 GO:0003006 GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005102 GO:0005488 GO:0005515 GO:0005543 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005766 GO:0005773 GO:0005774 GO:0005775 GO:0005886 GO:0006629 GO:0006643 GO:0006664 GO:0006665 GO:0006687 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006887 GO:0006955 GO:0007154 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0007193 GO:0007275 GO:0008047 GO:0008150 GO:0008152 GO:0008289 GO:0009888 GO:0009894 GO:0009987 GO:0010506 GO:0010876 GO:0012505 GO:0012506 GO:0015711 GO:0015849 GO:0015925 GO:0016020 GO:0016192 GO:0016787 GO:0016798 GO:0019216 GO:0019222 GO:0022414 GO:0023052 GO:0030141 GO:0030154 GO:0030234 GO:0030659 GO:0030667 GO:0030850 GO:0030855 GO:0031090 GO:0031323 GO:0031329 GO:0031406 GO:0031410 GO:0031974 GO:0031982 GO:0032501 GO:0032502 GO:0032940 GO:0033036 GO:0033218 GO:0033293 GO:0035265 GO:0035577 GO:0035594 GO:0035627 GO:0036094 GO:0036230 GO:0040007 GO:0042119 GO:0042582 GO:0042802 GO:0042803 GO:0042886 GO:0043085 GO:0043167 GO:0043168 GO:0043177 GO:0043202 GO:0043208 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043299 GO:0043312 GO:0044093 GO:0044237 GO:0044238 GO:0044255 GO:0044421 GO:0044422 GO:0044424 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045055 GO:0045321 GO:0046625 GO:0046836 GO:0046903 GO:0046942 GO:0046983 GO:0048513 GO:0048589 GO:0048608 GO:0048731 GO:0048732 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0051861 GO:0060429 GO:0060736 GO:0060742 GO:0061458 GO:0065007 GO:0065009 GO:0070013 GO:0071702 GO:0071704 GO:0071705 GO:0071944 GO:0080090 GO:0097001 GO:0097367 GO:0097708 GO:0098588 GO:0098772 GO:0098805 GO:0098852 GO:0099503 GO:1901135 GO:1901264 GO:1901564 GO:1903509 GO:1905572 GO:1905573 GO:1905574 GO:1905575 GO:1905576 GO:1905577
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
SapB_1 SapB_2

Protein Analysis

114

Amino Acids

12.63

Weight (kDa)

4.88

Isoelectric Point (pI)

46.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SapB_1 PF05184 62 - 96 1.9e-13 Saposin-like type B, region 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 30
Acc36I ACCTGC 2 cut(s) 284, 295
AccB7I CCANNNNNTGG 1 cut(s) 133
AcoI YGGCCR 1 cut(s) 208
AcsI RAATTY 1 cut(s) 323
AcuI CTGAAG 1 cut(s) 177
AcyI GRCGYC 1 cut(s) 27
AdeI CACNNNGTG 2 cut(s) 133, 192
AfeI AGCGCT 1 cut(s) 216
AfiI CCNNNNNNNGG 1 cut(s) 133
AgsI TTSAA 2 cut(s) 117, 298
AjnI CCWGG 1 cut(s) 129
AjuI GAANNNNNNNTTGG 2 cut(s) 150, 182
AluBI AGCT 1 cut(s) 155
AluI AGCT 1 cut(s) 155
Alw21I GWGCWC 1 cut(s) 188
Alw44I GTGCAC 1 cut(s) 184
Aor51HI AGCGCT 1 cut(s) 216
AoxI GGCC 2 cut(s) 124, 208
ApaLI GTGCAC 1 cut(s) 184
ApeKI GCWGC 2 cut(s) 62, 301
ApoI RAATTY 1 cut(s) 323
AspLEI GCGC 2 cut(s) 217, 291
AsuHPI GGTGA 2 cut(s) 242, 304
BaeGI GKGCMC 1 cut(s) 188
BalI TGGCCA 1 cut(s) 210
Bbv12I GWGCWC 1 cut(s) 188
BbvI GCAGC 2 cut(s) 74, 313
BciT130I CCWGG 1 cut(s) 131
BfaI CTAG 1 cut(s) 84
BfoI RGCGCY 1 cut(s) 218
BfuAI ACCTGC 2 cut(s) 284, 295
BisI GCNGC 2 cut(s) 63, 302
BlsI GCNGC 2 cut(s) 64, 303
Bme1390I CCNGG 1 cut(s) 131
BmrFI CCNGG 1 cut(s) 131
BmsI GCATC 1 cut(s) 70
BsaHI GRCGYC 1 cut(s) 27
BsaJI CCNNGG 1 cut(s) 21
Bsc4I CCNNNNNNNGG 1 cut(s) 133
BseBI CCWGG 1 cut(s) 131
BseDI CCNNGG 1 cut(s) 21
BseLI CCNNNNNNNGG 1 cut(s) 133
BseMII CTCAG 1 cut(s) 296
BseRI GAGGAG 1 cut(s) 212
BseSI GKGCMC 1 cut(s) 188
BseXI GCAGC 2 cut(s) 74, 313
BshFI GGCC 2 cut(s) 126, 210
BsiHKAI GWGCWC 1 cut(s) 188
BslI CCNNNNNNNGG 1 cut(s) 133
BsnI GGCC 2 cut(s) 126, 210
Bsp1286I GDGCHC 1 cut(s) 188
Bsp143I GATC 1 cut(s) 252
Bsp19I CCATGG 1 cut(s) 21
BspANI GGCC 2 cut(s) 126, 210
BspCNI CTCAG 1 cut(s) 295
BspMI ACCTGC 2 cut(s) 284, 295
BssECI CCNNGG 1 cut(s) 21
BssMI GATC 1 cut(s) 252
BssNI GRCGYC 1 cut(s) 27
BssT1I CCWWGG 1 cut(s) 21
Bst2UI CCWGG 1 cut(s) 131
BstACI GRCGYC 1 cut(s) 27
BstC8I GCNNGC 2 cut(s) 184, 208
BstDEI CTNAG 1 cut(s) 282
BstDSI CCRYGG 1 cut(s) 21
BstH2I RGCGCY 1 cut(s) 218
BstHHI GCGC 2 cut(s) 217, 291
BstKTI GATC 1 cut(s) 255
BstMBI GATC 1 cut(s) 252
BstMWI GCNNNNNNNGC 3 cut(s) 71, 80, 212
BstNI CCWGG 1 cut(s) 131
BstSCI CCNGG 1 cut(s) 129
BstSLI GKGCMC 1 cut(s) 188
BstV1I GCAGC 2 cut(s) 74, 313
BsuRI GGCC 2 cut(s) 126, 210
BtgI CCRYGG 1 cut(s) 21
BveI ACCTGC 2 cut(s) 284, 295
Cac8I GCNNGC 2 cut(s) 184, 208
CfoI GCGC 2 cut(s) 217, 291
CsiI ACCWGGT 1 cut(s) 129
CviAII CATG 1 cut(s) 22
CviJI RGCY 4 cut(s) 74, 126, 155, 210
CviKI_1 RGCY 4 cut(s) 74, 126, 155, 210
DdeI CTNAG 1 cut(s) 282
DpnI GATC 1 cut(s) 254
DpnII GATC 1 cut(s) 252
DraIII CACNNNGTG 2 cut(s) 133, 192
EaeI YGGCCR 1 cut(s) 208
Eco130I CCWWGG 1 cut(s) 21
Eco47III AGCGCT 1 cut(s) 216
Eco57I CTGAAG 1 cut(s) 177
EcoRII CCWGG 1 cut(s) 129
EcoT14I CCWWGG 1 cut(s) 21
ErhI CCWWGG 1 cut(s) 21
FaeI CATG 1 cut(s) 25
FaiI YATR 2 cut(s) 23, 340
FatI CATG 1 cut(s) 21
Fnu4HI GCNGC 2 cut(s) 63, 302
Fsp4HI GCNGC 2 cut(s) 63, 302
FspBI CTAG 1 cut(s) 84
GlaI GCGC 2 cut(s) 216, 290
GluI GCNGC 2 cut(s) 63, 302
HaeII RGCGCY 1 cut(s) 218
HaeIII GGCC 2 cut(s) 126, 210
HhaI GCGC 2 cut(s) 217, 291
Hin1I GRCGYC 1 cut(s) 27
Hin1II CATG 1 cut(s) 25
Hin6I GCGC 2 cut(s) 215, 289
HinP1I GCGC 2 cut(s) 215, 289
HphI GGTGA 2 cut(s) 242, 304
Hpy166II GTNNAC 1 cut(s) 186
Hpy188I TCNGA 2 cut(s) 32, 273
Hpy8I GTNNAC 1 cut(s) 186
Hpy99I CGWCG 1 cut(s) 260
HpyAV CCTTC 2 cut(s) 157, 327
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 1 cut(s) 186
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 80, 212
HpyF3I CTNAG 1 cut(s) 282
HpySE526I ACGT 1 cut(s) 27
Hsp92I GRCGYC 1 cut(s) 27
Hsp92II CATG 1 cut(s) 25
HspAI GCGC 2 cut(s) 215, 289
Kzo9I GATC 1 cut(s) 252
LpnPI CCDG 7 cut(s) 108, 116, 143, 192, 257, 289, 290
Lsp1109I GCAGC 2 cut(s) 74, 313
LweI GCATC 1 cut(s) 70
MabI ACCWGGT 1 cut(s) 129
MaeI CTAG 1 cut(s) 84
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 1 cut(s) 310
MalI GATC 1 cut(s) 254
MboI GATC 1 cut(s) 252
MboII GAAGA 1 cut(s) 170
MhlI GDGCHC 1 cut(s) 188
MlsI TGGCCA 1 cut(s) 210
MluCI AATT 1 cut(s) 323
MluNI TGGCCA 1 cut(s) 210
MnlI CCTC 1 cut(s) 190
Mox20I TGGCCA 1 cut(s) 210
MscI TGGCCA 1 cut(s) 210
MseI TTAA 1 cut(s) 6
Msp20I TGGCCA 1 cut(s) 210
MspR9I CCNGG 1 cut(s) 131
MvaI CCWGG 1 cut(s) 131
MwoI GCNNNNNNNGC 3 cut(s) 71, 80, 212
NcoI CCATGG 1 cut(s) 21
NdeII GATC 1 cut(s) 252
NlaIII CATG 1 cut(s) 25
NmuCI GTSAC 1 cut(s) 310
PflMI CCANNNNNTGG 1 cut(s) 133
PkrI GCNGC 2 cut(s) 64, 303
Psp6I CCWGG 1 cut(s) 129
PspGI CCWGG 1 cut(s) 129
SaqAI TTAA 1 cut(s) 6
SatI GCNGC 2 cut(s) 63, 302
Sau3AI GATC 1 cut(s) 252
ScrFI CCNGG 1 cut(s) 131
SduI GDGCHC 1 cut(s) 188
SetI ASST 8 cut(s) 30, 102, 132, 149, 157, 267, 278, 309
SexAI ACCWGGT 1 cut(s) 129
SfaNI GCATC 1 cut(s) 70
Sse9I AATT 1 cut(s) 323
SspMI CTAG 1 cut(s) 84
StyD4I CCNGG 1 cut(s) 129
StyI CCWWGG 1 cut(s) 21
TaiI ACGT 1 cut(s) 30
TaqI TCGA 1 cut(s) 258
TasI AATT 1 cut(s) 323
Tru1I TTAA 1 cut(s) 6
Tru9I TTAA 1 cut(s) 6
TseFI GTSAC 1 cut(s) 310
TseI GCWGC 2 cut(s) 62, 301
Tsp45I GTSAC 1 cut(s) 310
Van91I CCANNNNNTGG 1 cut(s) 133
VneI GTGCAC 1 cut(s) 184
XapI RAATTY 1 cut(s) 323
XspI CTAG 1 cut(s) 84
ZraI GACGTC 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.