MD12G1184900.v1.1

Saposin (B) Domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0012842
Physical Location & Seq
Forward (+)
26583786 .. 26587605
3820 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1184900.v1.1.491

Sequence Viewer

Length: 897 bp
ATGGCACGCACAAGCCCAACCCAGATGGGCAATTGGGTAGCTGGTTGTATAAAACAAAGAAGCAAAGCAAACGACCACAGCCGACAATTCAACGCTCCCCCAAACGCCCAGCCCCAACAAAGAAAGAAAGGAGGAATTCCTAGACATCAAGTTTTCCACTTCTTTCTTTCTCTCTCCTCACAATCGCAGGTTGTTTATGGCACCATGGACATGAGAGTTGGGGTTTTGTTTCTTTTTGTGCTGGCAGCTAGTTGGGCTTGTGATGCTAGACAACTGACGAACCTTGATCTGTCAGTTTCAAAGTCAGACCACCAGTTGGAAACTCAAACTTTCCAAGTTGAAGAAGTTGTTGGGAATGATAACGTGTGCACATTGTGTGAGGAGTTTGCTGACCAGGCACTCGATTACCTTAATGAAAACAAGACCCAGACGGAGATCATTGAATACCTACATCAGACCTGCCATCAGTTGCGCTCATTCAACCAGCAGTGTGTCACTTTGGTGGACTATTATGCTCCTCTCTTCTTCTTAGAGGCCACCTCTCTACAACCTTCTGAGTTCTGTCGGAAGGTCAATCTCTGTCAGCAAGTGGCTTTGTTTTCTTCACAATTCAAAGAGGATAGCTGTGGATTATGTCACCGTGCTGTTTCAGAAGTGTTGGTTAAGTTGAAGGATCCCGACACGCAGCTGGAGATTATTGAGTTGCTTCTGAAGGCATGCAATTCGGTGGAGAACTATACCAAAAAGTGCAAGAGGCTCGTTTTCGAGTATGGCCCCCTCATTCTTACCAATGCAGAGCAATTCCTCGAAACAACGGACATATGCACTGCGCTTCATGCCTGTAATTCGAACAAAGCTAGCATTACGGAACACATATCCAATCTGTCCGCCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000323 GO:0001655 GO:0001664 GO:0001775 GO:0002252 GO:0002263 GO:0002274 GO:0002275 GO:0002283 GO:0002366 GO:0002376 GO:0002443 GO:0002444 GO:0002446 GO:0002576 GO:0003006 GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005102 GO:0005488 GO:0005515 GO:0005543 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005766 GO:0005773 GO:0005774 GO:0005775 GO:0005886 GO:0006629 GO:0006643 GO:0006664 GO:0006665 GO:0006687 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006887 GO:0006955 GO:0007154 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0007193 GO:0007275 GO:0008047 GO:0008150 GO:0008152 GO:0008289 GO:0009888 GO:0009894 GO:0009987 GO:0010506 GO:0010876 GO:0012505 GO:0012506 GO:0015711 GO:0015849 GO:0015925 GO:0016020 GO:0016192 GO:0016787 GO:0016798 GO:0019216 GO:0019222 GO:0022414 GO:0023052 GO:0030141 GO:0030154 GO:0030234 GO:0030659 GO:0030667 GO:0030850 GO:0030855 GO:0031090 GO:0031323 GO:0031329 GO:0031406 GO:0031410 GO:0031974 GO:0031982 GO:0032501 GO:0032502 GO:0032940 GO:0033036 GO:0033218 GO:0033293 GO:0035265 GO:0035577 GO:0035594 GO:0035627 GO:0036094 GO:0036230 GO:0040007 GO:0042119 GO:0042582 GO:0042802 GO:0042803 GO:0042886 GO:0043085 GO:0043167 GO:0043168 GO:0043177 GO:0043202 GO:0043208 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043299 GO:0043312 GO:0044093 GO:0044237 GO:0044238 GO:0044255 GO:0044421 GO:0044422 GO:0044424 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045055 GO:0045321 GO:0046625 GO:0046836 GO:0046903 GO:0046942 GO:0046983 GO:0048513 GO:0048589 GO:0048608 GO:0048731 GO:0048732 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0051861 GO:0060429 GO:0060736 GO:0060742 GO:0061458 GO:0065007 GO:0065009 GO:0070013 GO:0071702 GO:0071704 GO:0071705 GO:0071944 GO:0080090 GO:0097001 GO:0097367 GO:0097708 GO:0098588 GO:0098772 GO:0098805 GO:0098852 GO:0099503 GO:1901135 GO:1901264 GO:1901564 GO:1903509 GO:1905572 GO:1905573 GO:1905574 GO:1905575 GO:1905576 GO:1905577
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
SapB_1 SapB_2

Protein Analysis

299

Amino Acids

33.66

Weight (kDa)

5.85

Isoelectric Point (pI)

45.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SapB_1 PF05184 122 - 157 2.3e-12 Saposin-like type B, region 1
SapB_1 PF05184 208 - 242 3.5e-09 Saposin-like type B, region 1
SapB_2 PF03489 248 - 281 5.1e-10 Saposin-like type B, region 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 178, 467
AccB1I GGYRCC 1 cut(s) 200
AccB7I CCANNNNNTGG 1 cut(s) 316
AciI CCGC 1 cut(s) 888
AclWI GGATC 2 cut(s) 668, 681
AcsI RAATTY 1 cut(s) 135
AcuI CTGAAG 1 cut(s) 731
AdeI CACNNNGTG 1 cut(s) 375
AfiI CCNNNNNNNGG 1 cut(s) 316
AflIII ACRYGT 1 cut(s) 363
AgsI TTSAA 7 cut(s) 91, 300, 341, 443, 481, 613, 670
AjnI CCWGG 1 cut(s) 393
AjuI GAANNNNNNNTTGG 2 cut(s) 333, 365
AleI CACNNNNGTG 1 cut(s) 500
AluBI AGCT 5 cut(s) 41, 248, 624, 688, 857
AluI AGCT 5 cut(s) 41, 248, 624, 688, 857
Alw21I GWGCWC 1 cut(s) 371
Alw44I GTGCAC 1 cut(s) 367
AlwI GGATC 2 cut(s) 668, 681
AoxI GGCC 2 cut(s) 534, 772
ApaLI GTGCAC 1 cut(s) 367
ApeKI GCWGC 2 cut(s) 245, 685
ApoI RAATTY 1 cut(s) 135
AspLEI GCGC 2 cut(s) 474, 832
AspS9I GGNCC 1 cut(s) 773
AsuHPI GGTGA 1 cut(s) 629
AsuII TTCGAA 1 cut(s) 848
AsuNHI GCTAGC 1 cut(s) 857
BaeGI GKGCMC 1 cut(s) 371
BamHI GGATCC 1 cut(s) 673
BanI GGYRCC 1 cut(s) 200
Bbv12I GWGCWC 1 cut(s) 371
BbvI GCAGC 2 cut(s) 257, 697
BccI CCATC 2 cut(s) 19, 471
BcgI CGANNNNNNTGC 4 cut(s) 705, 739, 739, 773
BciT130I CCWGG 1 cut(s) 395
BfaI CTAG 4 cut(s) 141, 249, 267, 858
BfuAI ACCTGC 2 cut(s) 178, 467
BisI GCNGC 2 cut(s) 246, 686
BlsI GCNGC 2 cut(s) 247, 687
Bme1390I CCNGG 1 cut(s) 395
BmgT120I GGNCC 1 cut(s) 773
BmiI GGNNCC 3 cut(s) 202, 675, 775
BmrFI CCNGG 1 cut(s) 395
BmsI GCATC 1 cut(s) 253
BmtI GCTAGC 1 cut(s) 861
BplI GAGNNNNNCTC 2 cut(s) 524, 556
BpmI CTGGAG 1 cut(s) 710
Bpu14I TTCGAA 1 cut(s) 848
BsaJI CCNNGG 1 cut(s) 204
Bsc4I CCNNNNNNNGG 1 cut(s) 316
Bse1I ACTGG 1 cut(s) 313
BseBI CCWGG 1 cut(s) 395
BseDI CCNNGG 1 cut(s) 204
BseLI CCNNNNNNNGG 1 cut(s) 316
BseMII CTCAG 1 cut(s) 546
BseNI ACTGG 1 cut(s) 313
BseRI GAGGAG 3 cut(s) 166, 395, 507
BseSI GKGCMC 1 cut(s) 371
BseXI GCAGC 2 cut(s) 257, 697
BseYI CCCAGC 1 cut(s) 108
BshFI GGCC 2 cut(s) 536, 774
BshNI GGYRCC 1 cut(s) 200
BsiHKAI GWGCWC 1 cut(s) 371
BslI CCNNNNNNNGG 1 cut(s) 316
BsnI GGCC 2 cut(s) 536, 774
Bsp119I TTCGAA 1 cut(s) 848
Bsp1286I GDGCHC 1 cut(s) 371
Bsp143I GATC 3 cut(s) 286, 435, 673
Bsp19I CCATGG 1 cut(s) 204
BspACI CCGC 1 cut(s) 888
BspANI GGCC 2 cut(s) 536, 774
BspCNI CTCAG 1 cut(s) 547
BspLI GGNNCC 3 cut(s) 202, 675, 775
BspMI ACCTGC 2 cut(s) 178, 467
BspOI GCTAGC 1 cut(s) 861
BspPI GGATC 2 cut(s) 668, 681
BspT104I TTCGAA 1 cut(s) 848
BspT107I GGYRCC 1 cut(s) 200
BsrI ACTGG 1 cut(s) 313
BssECI CCNNGG 1 cut(s) 204
BssMI GATC 3 cut(s) 286, 435, 673
BssT1I CCWWGG 1 cut(s) 204
Bst2UI CCWGG 1 cut(s) 395
Bst4CI ACNGT 1 cut(s) 641
Bst6I CTCTTC 1 cut(s) 527
BstBI TTCGAA 1 cut(s) 848
BstC8I GCNNGC 4 cut(s) 7, 243, 718, 859
BstDEI CTNAG 3 cut(s) 529, 555, 894
BstDSI CCRYGG 1 cut(s) 204
BstHHI GCGC 2 cut(s) 474, 832
BstKTI GATC 3 cut(s) 289, 438, 676
BstMBI GATC 3 cut(s) 286, 435, 673
BstMWI GCNNNNNNNGC 4 cut(s) 254, 263, 395, 836
BstNI CCWGG 1 cut(s) 395
BstNSI RCATGY 1 cut(s) 720
BstSCI CCNGG 1 cut(s) 393
BstSLI GKGCMC 1 cut(s) 371
BstV1I GCAGC 2 cut(s) 257, 697
BstX2I RGATCY 1 cut(s) 673
BstYI RGATCY 1 cut(s) 673
BsuRI GGCC 2 cut(s) 536, 774
BtgI CCRYGG 1 cut(s) 204
BtsI GCAGTG 2 cut(s) 494, 825
BtsIMutI CAGTG 2 cut(s) 494, 825
BveI ACCTGC 2 cut(s) 178, 467
Cac8I GCNNGC 4 cut(s) 7, 243, 718, 859
CfoI GCGC 2 cut(s) 474, 832
Cfr13I GGNCC 1 cut(s) 773
CviAII CATG 4 cut(s) 205, 211, 717, 836
DdeI CTNAG 3 cut(s) 529, 555, 894
DpnI GATC 3 cut(s) 288, 437, 675
DpnII GATC 3 cut(s) 286, 435, 673
DraIII CACNNNGTG 1 cut(s) 375
Eam1104I CTCTTC 1 cut(s) 527
EarI CTCTTC 1 cut(s) 527
EciI GGCGGA 1 cut(s) 877
Eco130I CCWWGG 1 cut(s) 204
Eco57I CTGAAG 1 cut(s) 731
EcoRI GAATTC 1 cut(s) 135
EcoRII CCWGG 1 cut(s) 393
EcoT14I CCWWGG 1 cut(s) 204
ErhI CCWWGG 1 cut(s) 204
FaeI CATG 4 cut(s) 208, 214, 720, 839
FatI CATG 4 cut(s) 204, 210, 716, 835
FauNDI CATATG 1 cut(s) 821
Fnu4HI GCNGC 2 cut(s) 246, 686
Fsp4HI GCNGC 2 cut(s) 246, 686
FspBI CTAG 4 cut(s) 141, 249, 267, 858
GlaI GCGC 2 cut(s) 473, 831
GluI GCNGC 2 cut(s) 246, 686
GsaI CCCAGC 1 cut(s) 112
GsuI CTGGAG 1 cut(s) 710
HaeIII GGCC 2 cut(s) 536, 774
HhaI GCGC 2 cut(s) 474, 832
Hin1II CATG 4 cut(s) 208, 214, 720, 839
Hin6I GCGC 2 cut(s) 472, 830
HinP1I GCGC 2 cut(s) 472, 830
HphI GGTGA 1 cut(s) 629
Hpy166II GTNNAC 2 cut(s) 369, 505
Hpy188I TCNGA 6 cut(s) 307, 456, 556, 567, 652, 711
Hpy188III TCNNGA 1 cut(s) 677
Hpy8I GTNNAC 2 cut(s) 369, 505
HpyAV CCTTC 4 cut(s) 561, 562, 664, 706
HpyCH4III ACNGT 1 cut(s) 641
HpyCH4IV ACGT 1 cut(s) 363
HpyCH4V TGCA 5 cut(s) 369, 720, 750, 794, 825
HpyF10VI GCNNNNNNNGC 4 cut(s) 254, 263, 395, 836
HpyF3I CTNAG 3 cut(s) 529, 555, 894
HpySE526I ACGT 1 cut(s) 363
Hsp92II CATG 4 cut(s) 208, 214, 720, 839
HspAI GCGC 2 cut(s) 472, 830
Kzo9I GATC 3 cut(s) 286, 435, 673
LmnI GCTCC 2 cut(s) 100, 520
Lsp1109I GCAGC 2 cut(s) 257, 697
LweI GCATC 1 cut(s) 253
MaeI CTAG 4 cut(s) 141, 249, 267, 858
MaeII ACGT 1 cut(s) 363
MaeIII GTNAC 2 cut(s) 493, 635
MalI GATC 3 cut(s) 288, 437, 675
MboI GATC 3 cut(s) 286, 435, 673
MboII GAAGA 4 cut(s) 353, 514, 517, 594
MfeI CAATTG 1 cut(s) 31
MflI RGATCY 1 cut(s) 673
MhlI GDGCHC 1 cut(s) 371
MluCI AATT 7 cut(s) 31, 86, 135, 608, 721, 800, 844
MmeI TCCRAC 2 cut(s) 297, 545
MseI TTAA 2 cut(s) 411, 663
MslI CAYNNNNRTG 2 cut(s) 209, 500
MspA1I CMGCKG 1 cut(s) 688
MspR9I CCNGG 1 cut(s) 395
MunI CAATTG 1 cut(s) 31
MvaI CCWGG 1 cut(s) 395
MwoI GCNNNNNNNGC 4 cut(s) 254, 263, 395, 836
NcoI CCATGG 1 cut(s) 204
NdeI CATATG 1 cut(s) 821
NdeII GATC 3 cut(s) 286, 435, 673
NheI GCTAGC 1 cut(s) 857
NlaIII CATG 4 cut(s) 208, 214, 720, 839
NlaIV GGNNCC 3 cut(s) 202, 675, 775
NmuCI GTSAC 2 cut(s) 493, 635
NspI RCATGY 1 cut(s) 720
NspV TTCGAA 1 cut(s) 848
OliI CACNNNNGTG 1 cut(s) 500
PaeI GCATGC 1 cut(s) 720
PflMI CCANNNNNTGG 1 cut(s) 316
PkrI GCNGC 2 cut(s) 247, 687
Psp6I CCWGG 1 cut(s) 393
PspFI CCCAGC 1 cut(s) 108
PspGI CCWGG 1 cut(s) 393
PspN4I GGNNCC 3 cut(s) 202, 675, 775
PspPI GGNCC 1 cut(s) 773
PsuI RGATCY 1 cut(s) 673
PvuII CAGCTG 1 cut(s) 688
RseI CAYNNNNRTG 2 cut(s) 209, 500
SaqAI TTAA 2 cut(s) 411, 663
SatI GCNGC 2 cut(s) 246, 686
Sau3AI GATC 3 cut(s) 286, 435, 673
Sau96I GGNCC 1 cut(s) 773
ScrFI CCNGG 1 cut(s) 395
SduI GDGCHC 1 cut(s) 371
SfaNI GCATC 1 cut(s) 253
SfuI TTCGAA 1 cut(s) 848
SmiMI CAYNNNNRTG 2 cut(s) 209, 500
SphI GCATGC 1 cut(s) 720
Sse9I AATT 7 cut(s) 31, 86, 135, 608, 721, 800, 844
SsiI CCGC 1 cut(s) 888
SspMI CTAG 4 cut(s) 141, 249, 267, 858
StyD4I CCNGG 1 cut(s) 393
StyI CCWWGG 1 cut(s) 204
TaaI ACNGT 1 cut(s) 641
TaiI ACGT 1 cut(s) 366
TaqI TCGA 4 cut(s) 402, 765, 807, 848
TasI AATT 7 cut(s) 31, 86, 135, 608, 721, 800, 844
Tru1I TTAA 2 cut(s) 411, 663
Tru9I TTAA 2 cut(s) 411, 663
TscAI CASTG 2 cut(s) 494, 832
TseFI GTSAC 2 cut(s) 493, 635
TseI GCWGC 2 cut(s) 245, 685
Tsp45I GTSAC 2 cut(s) 493, 635
TspDTI ATGAA 2 cut(s) 429, 824
TspGWI ACGGA 3 cut(s) 446, 830, 881
TspRI CASTG 2 cut(s) 494, 832
Van91I CCANNNNNTGG 1 cut(s) 316
VneI GTGCAC 1 cut(s) 367
XapI RAATTY 1 cut(s) 135
XceI RCATGY 1 cut(s) 720
XspI CTAG 4 cut(s) 141, 249, 267, 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.