FvH4_6g10220

Saposin (B) Domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0012842
Physical Location & Seq
Reverse (-)
6073900 .. 6077665
3766 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g10220.t2

Sequence Viewer

Length: 795 bp
ATGTATATAACAAAGCAAACAATTCAAGCAACTACAATTCAACGCCTTCGTTTGCTCAACCGAAAAACTCCCAACACTGAGAAGAACACCAATTTGAGGTACACCATGGATATGAGAGTTGGGTTTGTAATTCTTTTTGTGCTTGGAGCTAGTTGGGCTTGTGATGCTAGACATGTGGAGGAGCTTAGTTTGTCAGTGCAAGAAGGGGAACCTCAATCTTTGAAAGAGTTTTCTGGGAATGAAAATGTATGCACATTATGTGAGGAGTTTGCTTCTCAGGCACTTGATTACATAAGTGAAAACAAGACTCAGACTGAGATCATTGAATATCTACATAACTCCTGCTCTCAATTGAAATCTTTGAAGCAGCAGTGTGTCACTTTGGTGGACTATTATGCTCCTCTCTTCTTCCTAGAGGTTACCTCTTTAGAACCTGTTGAATTCTGCCGGAAGGTCAACCTATGTCAGCAAGTTGCAACGTTTTCTTCACAGCTCAAGGAGGACAGTTGTGGATTATGTCACCGTACTGTTTCAGAAGTATTAGTAAAGTTAAAAGATCCTGATACACAGCTGGAGATCATTGAGATTCTTTTGAAGGCATGCAATTCTGTGGAGAACTATGTCAAGAAGTGCAAAAGGCTTGTTTTTCAGTACGGACCACTAATCCTTGTGAATGCGGAGGAGTACCTTGAGAAAACAGACATATGCACTGCCCTTCATGCCTGTAATCCAAGCACAGTTAGTACAATGGAAGCATCATCGTTGGATATTGGATCCATGCGGGCCGACTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000323 GO:0001655 GO:0001664 GO:0001775 GO:0002252 GO:0002263 GO:0002274 GO:0002275 GO:0002283 GO:0002366 GO:0002376 GO:0002443 GO:0002444 GO:0002446 GO:0002576 GO:0003006 GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005102 GO:0005488 GO:0005515 GO:0005543 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005766 GO:0005773 GO:0005774 GO:0005775 GO:0005886 GO:0006629 GO:0006643 GO:0006664 GO:0006665 GO:0006687 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006887 GO:0006955 GO:0007154 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0007193 GO:0007275 GO:0008047 GO:0008150 GO:0008152 GO:0008289 GO:0009888 GO:0009894 GO:0009987 GO:0010506 GO:0010876 GO:0012505 GO:0012506 GO:0015711 GO:0015849 GO:0015925 GO:0016020 GO:0016192 GO:0016787 GO:0016798 GO:0019216 GO:0019222 GO:0022414 GO:0023052 GO:0030141 GO:0030154 GO:0030234 GO:0030659 GO:0030667 GO:0030850 GO:0030855 GO:0031090 GO:0031323 GO:0031329 GO:0031406 GO:0031410 GO:0031974 GO:0031982 GO:0032501 GO:0032502 GO:0032940 GO:0033036 GO:0033218 GO:0033293 GO:0035265 GO:0035577 GO:0035594 GO:0035627 GO:0036094 GO:0036230 GO:0040007 GO:0042119 GO:0042582 GO:0042802 GO:0042803 GO:0042886 GO:0043085 GO:0043167 GO:0043168 GO:0043177 GO:0043202 GO:0043208 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043299 GO:0043312 GO:0044093 GO:0044237 GO:0044238 GO:0044255 GO:0044421 GO:0044422 GO:0044424 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045055 GO:0045321 GO:0046625 GO:0046836 GO:0046903 GO:0046942 GO:0046983 GO:0048513 GO:0048589 GO:0048608 GO:0048731 GO:0048732 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0051861 GO:0060429 GO:0060736 GO:0060742 GO:0061458 GO:0065007 GO:0065009 GO:0070013 GO:0071702 GO:0071704 GO:0071705 GO:0071944 GO:0080090 GO:0097001 GO:0097367 GO:0097708 GO:0098588 GO:0098772 GO:0098805 GO:0098852 GO:0099503 GO:1901135 GO:1901264 GO:1901564 GO:1903509 GO:1905572 GO:1905573 GO:1905574 GO:1905575 GO:1905576 GO:1905577
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
SapB_1 SapB_2

Protein Analysis

265

Amino Acids

29.9

Weight (kDa)

5.17

Isoelectric Point (pI)

48.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SapB_1 PF05184 48 - 83 8.9e-13 Saposin-like type B, region 1
SapB_1 PF05184 134 - 168 1.2e-08 Saposin-like type B, region 1
SapB_2 PF03489 174 - 207 2.1e-09 Saposin-like type B, region 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 677, 781
AclI AACGTT 1 cut(s) 479
AclWI GGATC 3 cut(s) 551, 768, 781
AcsI RAATTY 1 cut(s) 440
AfaI GTAC 5 cut(s) 101, 526, 653, 686, 745
AfiI CCNNNNNNNGG 1 cut(s) 96
AflIII ACRYGT 1 cut(s) 172
AgsI TTSAA 8 cut(s) 26, 41, 223, 326, 355, 364, 440, 595
AleI CACNNNNGTG 1 cut(s) 383
AluBI AGCT 4 cut(s) 149, 184, 493, 571
AluI AGCT 4 cut(s) 149, 184, 493, 571
AlwI GGATC 3 cut(s) 551, 768, 781
AoxI GGCC 1 cut(s) 783
ApeKI GCWGC 1 cut(s) 367
ApoI RAATTY 1 cut(s) 440
AspS9I GGNCC 2 cut(s) 656, 783
AsuHPI GGTGA 1 cut(s) 512
AvaII GGWCC 1 cut(s) 656
BamHI GGATCC 1 cut(s) 773
BbvI GCAGC 1 cut(s) 379
BfaI CTAG 3 cut(s) 150, 168, 413
BisI GCNGC 1 cut(s) 368
BlsI GCNGC 1 cut(s) 369
Bme18I GGWCC 1 cut(s) 656
BmgT120I GGNCC 2 cut(s) 656, 783
BmiI GGNNCC 2 cut(s) 210, 775
BmsI GCATC 2 cut(s) 154, 764
BplI GAGNNNNNCTC 2 cut(s) 407, 439
BpmI CTGGAG 1 cut(s) 593
BpuEI CTTGAG 2 cut(s) 479, 710
BsaJI CCNNGG 1 cut(s) 105
Bsc4I CCNNNNNNNGG 1 cut(s) 96
BseDI CCNNGG 1 cut(s) 105
BseLI CCNNNNNNNGG 1 cut(s) 96
BseMII CTCAG 4 cut(s) 69, 290, 306, 323
BseRI GAGGAG 4 cut(s) 194, 278, 390, 695
BseXI GCAGC 1 cut(s) 379
BshFI GGCC 1 cut(s) 785
BsiSI CCGG 1 cut(s) 448
BslI CCNNNNNNNGG 1 cut(s) 96
BsmI GAATGC 1 cut(s) 679
BsnI GGCC 1 cut(s) 785
Bsp143I GATC 4 cut(s) 318, 556, 576, 773
Bsp19I CCATGG 1 cut(s) 105
BspACI CCGC 2 cut(s) 677, 781
BspANI GGCC 1 cut(s) 785
BspCNI CTCAG 4 cut(s) 70, 289, 307, 322
BspLI GGNNCC 2 cut(s) 210, 775
BspPI GGATC 3 cut(s) 551, 768, 781
BssECI CCNNGG 1 cut(s) 105
BssMI GATC 4 cut(s) 318, 556, 576, 773
BssT1I CCWWGG 1 cut(s) 105
Bst4CI ACNGT 4 cut(s) 506, 524, 529, 739
Bst6I CTCTTC 1 cut(s) 410
BstC8I GCNNGC 2 cut(s) 601, 783
BstDEI CTNAG 6 cut(s) 78, 185, 276, 309, 315, 792
BstDSI CCRYGG 1 cut(s) 105
BstEII GGTNACC 1 cut(s) 418
BstKTI GATC 4 cut(s) 321, 559, 579, 776
BstMBI GATC 4 cut(s) 318, 556, 576, 773
BstMWI GCNNNNNNNGC 4 cut(s) 155, 164, 278, 719
BstNSI RCATGY 2 cut(s) 176, 603
BstPI GGTNACC 1 cut(s) 418
BstV1I GCAGC 1 cut(s) 379
BstX2I RGATCY 2 cut(s) 556, 773
BstYI RGATCY 2 cut(s) 556, 773
BsuRI GGCC 1 cut(s) 785
BtgI CCRYGG 1 cut(s) 105
BtsI GCAGTG 2 cut(s) 377, 708
BtsIMutI CAGTG 4 cut(s) 75, 201, 377, 708
Cac8I GCNNGC 2 cut(s) 601, 783
Cfr13I GGNCC 2 cut(s) 656, 783
Csp6I GTAC 5 cut(s) 100, 525, 652, 685, 744
CviAII CATG 5 cut(s) 106, 173, 600, 719, 778
CviJI RGCY 7 cut(s) 149, 158, 184, 493, 571, 640, 785
CviKI_1 RGCY 7 cut(s) 149, 158, 184, 493, 571, 640, 785
CviQI GTAC 5 cut(s) 100, 525, 652, 685, 744
DdeI CTNAG 6 cut(s) 78, 185, 276, 309, 315, 792
DpnI GATC 4 cut(s) 320, 558, 578, 775
DpnII GATC 4 cut(s) 318, 556, 576, 773
Eam1104I CTCTTC 1 cut(s) 410
EarI CTCTTC 1 cut(s) 410
Eco130I CCWWGG 1 cut(s) 105
Eco47I GGWCC 1 cut(s) 656
Eco91I GGTNACC 1 cut(s) 418
EcoO65I GGTNACC 1 cut(s) 418
EcoRI GAATTC 1 cut(s) 440
EcoT14I CCWWGG 1 cut(s) 105
ErhI CCWWGG 1 cut(s) 105
FaeI CATG 5 cut(s) 109, 176, 603, 722, 781
FatI CATG 5 cut(s) 105, 172, 599, 718, 777
FauI CCCGC 1 cut(s) 774
FauNDI CATATG 1 cut(s) 704
Fnu4HI GCNGC 1 cut(s) 368
Fsp4HI GCNGC 1 cut(s) 368
FspBI CTAG 3 cut(s) 150, 168, 413
GluI GCNGC 1 cut(s) 368
GsuI CTGGAG 1 cut(s) 593
HaeIII GGCC 1 cut(s) 785
HapII CCGG 1 cut(s) 448
Hin1II CATG 5 cut(s) 109, 176, 603, 722, 781
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HinfI GANTC 3 cut(s) 307, 586, 788
HpaII CCGG 1 cut(s) 448
HphI GGTGA 1 cut(s) 512
Hpy166II GTNNAC 3 cut(s) 102, 388, 457
Hpy188I TCNGA 2 cut(s) 312, 535
Hpy188III TCNNGA 2 cut(s) 560, 625
Hpy8I GTNNAC 3 cut(s) 102, 388, 457
HpyAV CCTTC 5 cut(s) 56, 197, 445, 589, 725
HpyCH4III ACNGT 4 cut(s) 506, 524, 529, 739
HpyCH4IV ACGT 1 cut(s) 479
HpyCH4V TGCA 6 cut(s) 199, 252, 476, 603, 633, 708
HpyF10VI GCNNNNNNNGC 4 cut(s) 155, 164, 278, 719
HpyF3I CTNAG 6 cut(s) 78, 185, 276, 309, 315, 792
HpySE526I ACGT 1 cut(s) 479
Hsp92II CATG 5 cut(s) 109, 176, 603, 722, 781
Kzo9I GATC 4 cut(s) 318, 556, 576, 773
LmnI GCTCC 3 cut(s) 146, 181, 403
LpnPI CCDG 8 cut(s) 219, 263, 355, 447, 461, 557, 573, 736
Lsp1109I GCAGC 1 cut(s) 379
LweI GCATC 2 cut(s) 154, 764
MaeI CTAG 3 cut(s) 150, 168, 413
MaeII ACGT 1 cut(s) 479
MaeIII GTNAC 3 cut(s) 376, 418, 518
MalI GATC 4 cut(s) 320, 558, 578, 775
MboI GATC 4 cut(s) 318, 556, 576, 773
MboII GAAGA 4 cut(s) 94, 397, 400, 477
MfeI CAATTG 1 cut(s) 350
MflI RGATCY 2 cut(s) 556, 773
MluCI AATT 7 cut(s) 21, 36, 91, 129, 350, 440, 604
MlyI GAGTC 2 cut(s) 301, 782
MmeI TCCRAC 1 cut(s) 744
MnlI CCTC 9 cut(s) 90, 172, 222, 256, 409, 411, 433, 493, 673
MseI TTAA 1 cut(s) 551
MslI CAYNNNNRTG 2 cut(s) 110, 383
MspA1I CMGCKG 1 cut(s) 571
MspI CCGG 1 cut(s) 448
MunI CAATTG 1 cut(s) 350
Mva1269I GAATGC 1 cut(s) 679
MwoI GCNNNNNNNGC 4 cut(s) 155, 164, 278, 719
NcoI CCATGG 1 cut(s) 105
NdeI CATATG 1 cut(s) 704
NdeII GATC 4 cut(s) 318, 556, 576, 773
NlaIII CATG 5 cut(s) 109, 176, 603, 722, 781
NlaIV GGNNCC 2 cut(s) 210, 775
NmuCI GTSAC 2 cut(s) 376, 518
NspI RCATGY 2 cut(s) 176, 603
OliI CACNNNNGTG 1 cut(s) 383
PaeI GCATGC 1 cut(s) 603
PciI ACATGT 1 cut(s) 172
PctI GAATGC 1 cut(s) 679
PfeI GAWTC 1 cut(s) 586
PkrI GCNGC 1 cut(s) 369
PleI GAGTC 2 cut(s) 301, 782
PpsI GAGTC 2 cut(s) 301, 782
PscI ACATGT 1 cut(s) 172
Psp1406I AACGTT 1 cut(s) 479
PspEI GGTNACC 1 cut(s) 418
PspN4I GGNNCC 2 cut(s) 210, 775
PspPI GGNCC 2 cut(s) 656, 783
PsuI RGATCY 2 cut(s) 556, 773
PvuII CAGCTG 1 cut(s) 571
RsaI GTAC 5 cut(s) 101, 526, 653, 686, 745
RsaNI GTAC 5 cut(s) 100, 525, 652, 685, 744
RseI CAYNNNNRTG 2 cut(s) 110, 383
SaqAI TTAA 1 cut(s) 551
SatI GCNGC 1 cut(s) 368
Sau3AI GATC 4 cut(s) 318, 556, 576, 773
Sau96I GGNCC 2 cut(s) 656, 783
SchI GAGTC 2 cut(s) 301, 782
SfaNI GCATC 2 cut(s) 154, 764
SinI GGWCC 1 cut(s) 656
SmiMI CAYNNNNRTG 2 cut(s) 110, 383
SmlI CTYRAG 2 cut(s) 494, 689
SmoI CTYRAG 2 cut(s) 494, 689
SphI GCATGC 1 cut(s) 603
Sse9I AATT 7 cut(s) 21, 36, 91, 129, 350, 440, 604
SsiI CCGC 2 cut(s) 677, 781
SspMI CTAG 3 cut(s) 150, 168, 413
StyI CCWWGG 1 cut(s) 105
TaaI ACNGT 4 cut(s) 506, 524, 529, 739
TaiI ACGT 1 cut(s) 482
TasI AATT 7 cut(s) 21, 36, 91, 129, 350, 440, 604
TatI WGTACW 1 cut(s) 743
TfiI GAWTC 1 cut(s) 586
Tru1I TTAA 1 cut(s) 551
Tru9I TTAA 1 cut(s) 551
TscAI CASTG 4 cut(s) 82, 201, 377, 715
TseFI GTSAC 2 cut(s) 376, 518
TseI GCWGC 1 cut(s) 367
Tsp45I GTSAC 2 cut(s) 376, 518
TspDTI ATGAA 2 cut(s) 255, 707
TspGWI ACGGA 1 cut(s) 669
TspRI CASTG 4 cut(s) 82, 201, 377, 715
VpaK11BI GGWCC 1 cut(s) 656
XapI RAATTY 1 cut(s) 440
XceI RCATGY 2 cut(s) 176, 603
XspI CTAG 3 cut(s) 150, 168, 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.