AT3G53090

Ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0010433
Physical Location & Seq
Forward (+)
19679177 .. 19685360
6184 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G53090.1

Sequence Viewer

Length: 3429 bp
ATGGACCTGAATCGCAAACACAAGGTGTCTCTTAGAGGTGCCAGCAGTGGGGAGATTTCAAGGGATGCTTTGCTTGCTAAAGTGTCTCAAGAAAGGGAGCTACGCAGTTATGCAAGACGCGCTAATGCTGCATCACTTTTTATTCAAAGAGTTTGGAGGTCTTACATTGTGAGAAAGAAGGCAGCTATAGAGATCCAAGAGGAGTGGGAGAATTTACTGAGTTGCCATTCTGTTACACTGACCAAAAGTTGGGTATCAAGCAGAGTATTGAGACCGTTTCTCTTCTTTGTCAGATCTTTGTCTGTCCAACATCAAAAGATACAGGCAAGAGAAATACACTGCATGCAAACTTGCTTTAAGATTCTATTGGAAAGCATCAACTCTAATGATCAGGGGTACAATTTTTGTTCACTAGCAGTTGGTACATCTGAAGATAGTAAAACATGGGCCTGTCAAACACGAAGGATGGTTTCTCTCTGCTCTTTCCTTCTCACGGAGTGTAATTATTCGCAGGAAAGGATAAAAGATGTTATTGGTGTCAATGCCCTTCTATTACGTATTCTTATTGTTTTAACTGATCCCAAAAGCTGGAAGATTATTACAAATGAAAACTTTGAAGATGCAGAAACAGCTAAAAAGATAATTATTCAGTTTATAGGGAGCTGTAAAAGTGGATATTACTCAGCAGTTCGGAGATATATCAAGACATTAACCAAACATACAGACGAGAGACTAGTGATTACTACAAGTGCAGTAACTTTGGCTCTACGACCATTCCACGTGAAACAGCCTGCTTTTGTTGATGACAACCAACCGGACACGAATTTGGCTGTTGAAGAATATGTTTCTCTAATACTGACGATTCCTCGGCTTGTTTGCTACCTGCCAAGCGCTCTCATACGTGCACTGAAGCACAAGAGCATACTAATGCCAAGCTTTCACACCATATTGCTTTTGAAGGACAAAATCTTGAACATAATATCGGAAATGGAGAATTCAGAGAAGCAAAGTTGTACCATGGAGATACCTTCTGTTGGATGGGTTATCGGGAACATCATATCCCTCGCAACAGTTAGTGAAACCGATTTTATGGATCCACAAGAATCAAATCCAGAGATGTTTTATGTCTTGTATGTTCATGTTATTGTGACTCTCGCCGAGAATCTATTGTCTCAGGTTGAAAGTGTTGGAATTCAGGATATTCATCTTGATATTGAGGCCACATCGAATGAGACAGAGAAAGGAAATTCAGTTAAGATTTCTTTTGTGGAAATGCTTCGACCAGTATGTCAACAGTGGCATCTCGCAAAACTGTTGGCAGCTTCTGGGAAAGAAATTCGTGTTATAGCAGATAAAGATGCATCAACGAGTAGTAAGAAAGGATCAGAAACACTAGGACTGCTTGATATAGCACGATTATATTCATGCATGTTGAGAATCTTCTGCGTGATGAATCCTGTTCTAGGACCTTTGCCTGTCCTTAACATGTTATCCTTCTGTCCTGGATACATTGTCTCTTTGTGGAATTCTCTAGAGAGTGTCCTACTCCCTGAAAATGGTTGCACTGCTGACGATGCATCTCATGGATCTGCTAAAACTTCATGGAATACAAGAAGTCCTTCTGAGAAGAAATTGAAACATCTCAAGAATGACAGTGTCAACAAGTGGGTGAATGTGCTAAACAAGTTTTCTGGTAAATCACCGGGACCACGTGAACATGTGGAGTGTACTAGTGATCAACCAGGATCAGGCCAGGTTAATGAGTCAACTAATGATGTATGGGATGTGGAAACTCTGAGGGGTGGTCCTGTAGGTATCTCCAAAGAAGTGTCATGCCTGCTTCATCTATTTTGTGCAACCTATGCACATTTACTCGTTGTTCTTGATGACATACAGTTTTATGAAAAGCAGGTCCCCTTTATGCTAGAGAAGCAACAGAGAATTGCATCAATGCTTAATACACTCGTGTACTATGGATTGTTGCGAGGCACAGGTCCTGAGAGTAGACAACTGATGGATTCTGCTATCAGATGTTTGCATTTATTGTATGAAAGAGATTGCAGGCACCCTTTTTGTGCTTCTGCCCTGTGGCTCTCACCTGGTAGAACTAGTAGACCTCCAATTGCATTTGCTGCCAGGACTCATGAGGTTTTACCAACCAGTGATGTTCTTACAACTCCAAGCATGGGTTCTGTTATAACAATTACTCCGCATGTCTTTCCATTTGAGGAGAGGGTCCATGTGTTTAGAGAGTTTATAAGCAAGGATAAAGCTTCAAGGAAAATGGCTGGTGAAGTGGATGCACCTGGTGCGAGGTCAATAGAGATAGTTGTTCGTCGAGGTCATGTGGTTGAAGATGGATTTCAACAACTGAATTCAATTGGTAGCAGGCTAAAATCATCCATCCATGTCTCGTTTGTGAATGAATCTGGCCTTCCTGAGGCTGGCCTAGACTATGGTGGGCTTTCAAAAGAGTTTTTGACTGACATAACAAAAGCAGCTTTTGCTACTGAGTATGGGTTATTCTCGCAAACCCCTACTTCAGATAGGTTGCTTGTTCCTAGCCCATCTGCACGGCACCTTGAGAATGGGATTCAGATGATTGAGTTTCTTGGAAGAATTGTTGGAAAAGCTCTATATGAGGGAATACTGCTGGATTACTCTTTTTCACACGTTTTCATCCAGAAGCTTTTGGGGCGTTATAGCTTTATTGATGAACTATCAGGGCTTGATCCAGAGCTCTACAGGAACCTCATGTATGTCAAGCATTATGATGGTGATTTGAAGGAACTCTGTCTGGATTTCACAGTCACTGAAGAGTTTTGTGGAAAAATGAGCATAATTGAACTCAAACCCGGTGGGAAGGATACTTCAGTCACGAATGAGAACAAAATGCAATATATTCATGCAATGGCTGACTATAAACTTAACCGGCAGATTGTGCCCTTTTCAAATGCATTTTACAGAGGATTGACTGACCTTATATCTCCTGCTTGGTTGAAACTATTTAATGCCCATGAATTTAATCAGTTACTCTCAGGAGGAAACCATGATATTGATGTTGATGACTTAAGGAGGAATACAAAATACACTGGTGGTTACAGTGATAGCAGTCGAACAATCAAAATCTTCTGGGAGGTCATGAAAGGCTTTGAACCGAGTGAACGTTGCTTGCTCCTCAAATTTGTGACTAGTTGTTCTCGGGCACCACTCCTTGGTTTCAAATACTTGCAGCCAACCTTCATCATCCATAAAGTCTCTTGTGATACCTCTCTTTGGGCTGCTATTGGAGGACAAGATGTAGAAAGACTTCCATCTGCTTCTACGTGCTACAATACTCTTAAGCTTCCAACGTATAAACGAGCAAGCACTATGAGGGAAAAACTATTGTATGCGATTACTTCAAATGCAGGATTTGAACTCTCGTAG

Protein Analysis

1142

Amino Acids

128.48

Weight (kDa)

7.86

Isoelectric Point (pI)

45.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HECT PF00632 829 - 1142 2.2e-97 HECT-domain (ubiquitin-transferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2198, 2258
AasI GACNNNNNNGTC 1 cut(s) 1287
Acc36I ACCTGC 2 cut(s) 891, 1900
AccB1I GGYRCC 4 cut(s) 38, 2064, 2577, 3205
AccB7I CCANNNNNTGG 3 cut(s) 249, 588, 3215
AccI GTMKAC 2 cut(s) 2005, 2113
AccII CGCG 1 cut(s) 120
AciI CCGC 1 cut(s) 2210
AclI AACGTT 1 cut(s) 3166
AclWI GGATC 8 cut(s) 187, 572, 1088, 1101, 1390, 1594, 1753, 2726
AcuI CTGAAG 5 cut(s) 450, 929, 2526, 2835, 2856
AcvI CACGTG 2 cut(s) 781, 1712
AdeI CACNNNGTG 3 cut(s) 25, 498, 2309
AfaI GTAC 5 cut(s) 398, 424, 1015, 1729, 1970
AfeI AGCGCT 1 cut(s) 892
AflII CTTAAG 2 cut(s) 3070, 3341
AflIII ACRYGT 3 cut(s) 1485, 1717, 2671
AhlI ACTAGT 4 cut(s) 733, 1730, 2108, 3191
AjnI CCWGG 6 cut(s) 1501, 1741, 1752, 2098, 2135, 2305
AjuI GAANNNNNNNTTGG 2 cut(s) 2173, 2205
AloI GAACNNNNNNTCC 2 cut(s) 1043, 1075
Alw21I GWGCWC 2 cut(s) 907, 2742
Alw26I GTCTC 9 cut(s) 33, 90, 265, 724, 1176, 1226, 1519, 2416, 3262
Alw44I GTGCAC 1 cut(s) 903
AlwI GGATC 8 cut(s) 187, 572, 1088, 1101, 1390, 1594, 1753, 2726
AlwNI CAGNNNCTG 3 cut(s) 1325, 1997, 2813
Ama87I CYCGRG 1 cut(s) 3201
Aor51HI AGCGCT 1 cut(s) 892
AoxI GGCC 5 cut(s) 447, 1218, 1750, 2431, 2446
ApaLI GTGCAC 1 cut(s) 903
ApeKI GCWGC 7 cut(s) 128, 182, 1319, 2132, 2498, 3232, 3281
Asp700I GAANNNNTTC 3 cut(s) 1275, 1618, 3309
AspLEI GCGC 2 cut(s) 122, 893
AspS9I GGNCC 8 cut(s) 4, 447, 1466, 1706, 1805, 1912, 1994, 2236
AsuC2I CCSGG 2 cut(s) 1704, 2856
AsuHPI GGTGA 5 cut(s) 1681, 1692, 2088, 2303, 2789
AvaI CYCGRG 1 cut(s) 3201
AvaII GGWCC 7 cut(s) 4, 1466, 1706, 1805, 1912, 1994, 2236
AxyI CCTNAGG 1 cut(s) 2439
BaeGI GKGCMC 3 cut(s) 907, 2946, 3208
BaeI ACNNNNGTAYC 2 cut(s) 2859, 2892
BamHI GGATCC 1 cut(s) 1093
BanI GGYRCC 4 cut(s) 38, 2064, 2577, 3205
BanII GRGCYC 1 cut(s) 2742
BarI GAAGNNNNNNTAC 2 cut(s) 3294, 3326
BauI CACGAG 1 cut(s) 1964
BbrPI CACGTG 2 cut(s) 781, 1712
Bbv12I GWGCWC 2 cut(s) 907, 2742
BbvI GCAGC 7 cut(s) 115, 194, 1331, 2119, 2510, 3244, 3268
BccI CCATC 8 cut(s) 460, 1032, 2008, 2351, 2411, 2575, 2768, 3322
BceAI ACGGC 1 cut(s) 2591
BciT130I CCWGG 6 cut(s) 1503, 1743, 1754, 2100, 2137, 2307
BciVI GTATCC 2 cut(s) 1499, 2860
BclI TGATCA 2 cut(s) 388, 1735
BcnI CCSGG 2 cut(s) 1704, 2856
BcoDI GTCTC 9 cut(s) 33, 90, 265, 724, 1176, 1226, 1519, 2416, 3262
BcuI ACTAGT 4 cut(s) 733, 1730, 2108, 3191
BfmI CTRYAG 3 cut(s) 186, 1809, 2743
BfoI RGCGCY 1 cut(s) 894
BfrI CTTAAG 2 cut(s) 3070, 3341
BfuAI ACCTGC 2 cut(s) 891, 1900
BfuI GTATCC 2 cut(s) 1499, 2860
BglII AGATCT 1 cut(s) 293
BisI GCNGC 7 cut(s) 129, 183, 1320, 2133, 2499, 3233, 3282
BlsI GCNGC 7 cut(s) 130, 184, 1321, 2134, 2500, 3234, 3283
Bme1390I CCNGG 8 cut(s) 1503, 1704, 1743, 1754, 2100, 2137, 2307, 2856
Bme18I GGWCC 7 cut(s) 4, 1466, 1706, 1805, 1912, 1994, 2236
BmeT110I CYCGRG 1 cut(s) 3201
BmgT120I GGNCC 8 cut(s) 4, 447, 1466, 1706, 1805, 1912, 1994, 2236
BmiI GGNNCC 9 cut(s) 40, 1095, 1707, 1914, 2066, 2237, 2579, 2750, 3207
BmrFI CCNGG 8 cut(s) 1503, 1704, 1743, 1754, 2100, 2137, 2307, 2856
BpuEI CTTGAG 3 cut(s) 72, 1628, 2603
BpuMI CCSGG 2 cut(s) 1704, 2856
BsaAI YACGTR 5 cut(s) 557, 781, 902, 1712, 3327
BsaBI GATNNNNATC 1 cut(s) 1203
BsaI GGTCTC 1 cut(s) 265
BsaJI CCNNGG 3 cut(s) 866, 1017, 3214
BsaWI WCCGGW 1 cut(s) 814
BsaXI ACNNNNNCTCC 2 cut(s) 2191, 2221
Bse118I RCCGGY 1 cut(s) 2931
Bse1I ACTGG 3 cut(s) 1283, 2160, 3097
Bse21I CCTNAGG 1 cut(s) 2439
Bse3DI GCAATG 1 cut(s) 2916
Bse8I GATNNNNATC 1 cut(s) 1203
BseBI CCWGG 6 cut(s) 1503, 1743, 1754, 2100, 2137, 2307
BseDI CCNNGG 3 cut(s) 866, 1017, 3214
BseGI GGATG 9 cut(s) 70, 471, 1043, 1789, 2305, 2399, 2403, 2679, 3246
BseJI GATNNNNATC 1 cut(s) 1203
BseMI GCAATG 1 cut(s) 2916
BseMII CTCAG 9 cut(s) 209, 696, 1187, 1614, 1787, 1989, 2430, 2502, 3051
BseNI ACTGG 3 cut(s) 1283, 2160, 3097
BseRI GAGGAG 3 cut(s) 215, 2243, 3167
BseSI GKGCMC 3 cut(s) 907, 2946, 3208
BseXI GCAGC 7 cut(s) 115, 194, 1331, 2119, 2510, 3244, 3268
BsgI GTGCAG 2 cut(s) 771, 2556
Bsh1236I CGCG 1 cut(s) 120
BshFI GGCC 5 cut(s) 449, 1220, 1752, 2433, 2448
BshNI GGYRCC 4 cut(s) 38, 2064, 2577, 3205
BsiHKAI GWGCWC 2 cut(s) 907, 2742
BsiHKCI CYCGRG 1 cut(s) 3201
BsiSI CCGG 4 cut(s) 815, 1703, 2856, 2932
BslFI GGGAC 2 cut(s) 1719, 1898
BsmAI GTCTC 9 cut(s) 33, 90, 265, 724, 1176, 1226, 1519, 2416, 3262
BsmFI GGGAC 2 cut(s) 1719, 1898
BsnI GGCC 5 cut(s) 449, 1220, 1752, 2433, 2448
Bso31I GGTCTC 1 cut(s) 265
BsoBI CYCGRG 1 cut(s) 3201
Bsp1286I GDGCHC 4 cut(s) 907, 2742, 2946, 3208
Bsp19I CCATGG 1 cut(s) 1017
BspACI CCGC 1 cut(s) 2210
BspANI GGCC 5 cut(s) 449, 1220, 1752, 2433, 2448
BspCNI CTCAG 9 cut(s) 210, 695, 1186, 1615, 1788, 1990, 2431, 2503, 3050
BspFNI CGCG 1 cut(s) 120
BspHI TCATGA 2 cut(s) 2143, 3141
BspLI GGNNCC 9 cut(s) 40, 1095, 1707, 1914, 2066, 2237, 2579, 2750, 3207
BspMI ACCTGC 2 cut(s) 891, 1900
BspPI GGATC 8 cut(s) 187, 572, 1088, 1101, 1390, 1594, 1753, 2726
BspT107I GGYRCC 4 cut(s) 38, 2064, 2577, 3205
BspTI CTTAAG 2 cut(s) 3070, 3341
BspTNI GGTCTC 1 cut(s) 265
BsrDI GCAATG 1 cut(s) 2916
BsrFI RCCGGY 1 cut(s) 2931
BsrI ACTGG 3 cut(s) 1283, 2160, 3097
BssAI RCCGGY 1 cut(s) 2931
BssECI CCNNGG 3 cut(s) 866, 1017, 3214
BssSI CACGAG 1 cut(s) 1964
BssT1I CCWWGG 2 cut(s) 1017, 3214
Bst2BI CACGAG 1 cut(s) 1964
Bst2UI CCWGG 6 cut(s) 1503, 1743, 1754, 2100, 2137, 2307
Bst4CI ACNGT 8 cut(s) 276, 1072, 1296, 1314, 1655, 1896, 2809, 3104
Bst6I CTCTTC 2 cut(s) 287, 2811
BstAFI CTTAAG 2 cut(s) 3070, 3341
BstAPI GCANNNNNTGC 5 cut(s) 1862, 2132, 2309, 2504, 2941
BstBAI YACGTR 5 cut(s) 557, 781, 902, 1712, 3327
BstDSI CCRYGG 1 cut(s) 1017
BstENI CCTNNNNNAGG 2 cut(s) 1461, 2438
BstF5I GGATG 9 cut(s) 70, 471, 1043, 1789, 2305, 2399, 2403, 2679, 3246
BstFNI CGCG 1 cut(s) 120
BstH2I RGCGCY 1 cut(s) 894
BstHHI GCGC 2 cut(s) 122, 893
BstMAI GTCTC 9 cut(s) 33, 90, 265, 724, 1176, 1226, 1519, 2416, 3262
BstNI CCWGG 6 cut(s) 1503, 1743, 1754, 2100, 2137, 2307
BstNSI RCATGY 5 cut(s) 346, 1432, 1489, 1721, 2216
BstSCI CCNGG 8 cut(s) 1501, 1702, 1741, 1752, 2098, 2135, 2305, 2854
BstSFI CTRYAG 3 cut(s) 186, 1809, 2743
BstSLI GKGCMC 3 cut(s) 907, 2946, 3208
BstSNI TACGTA 1 cut(s) 557
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 7 cut(s) 115, 194, 1331, 2119, 2510, 3244, 3268
BstX2I RGATCY 4 cut(s) 192, 293, 1093, 1586
BstYI RGATCY 4 cut(s) 192, 293, 1093, 1586
Bsu36I CCTNAGG 1 cut(s) 2439
BsuI GTATCC 2 cut(s) 1499, 2860
BsuRI GGCC 5 cut(s) 449, 1220, 1752, 2433, 2448
BtgI CCRYGG 1 cut(s) 1017
BtsCI GGATG 9 cut(s) 70, 471, 1043, 1789, 2305, 2399, 2403, 2679, 3246
BtsI GCAGTG 3 cut(s) 52, 337, 1563
BveI ACCTGC 2 cut(s) 891, 1900
CaiI CAGNNNCTG 3 cut(s) 1325, 1997, 2813
CciI TCATGA 2 cut(s) 2143, 3141
CfoI GCGC 2 cut(s) 122, 893
Cfr10I RCCGGY 1 cut(s) 2931
Cfr13I GGNCC 8 cut(s) 4, 447, 1466, 1706, 1805, 1912, 1994, 2236
CseI GACGC 1 cut(s) 126
CsiI ACCWGGT 2 cut(s) 2098, 2305
Csp6I GTAC 5 cut(s) 397, 423, 1014, 1728, 1969
CspCI CAANNNNNGTGG 4 cut(s) 185, 220, 2839, 2874
CviQI GTAC 5 cut(s) 397, 423, 1014, 1728, 1969
DraIII CACNNNGTG 3 cut(s) 25, 498, 2309
DrdI GACNNNNNNGTC 1 cut(s) 1287
DseDI GACNNNNNNGTC 1 cut(s) 1287
Eam1104I CTCTTC 2 cut(s) 287, 2811
EarI CTCTTC 2 cut(s) 287, 2811
Ecl136II GAGCTC 1 cut(s) 2740
Eco105I TACGTA 1 cut(s) 557
Eco130I CCWWGG 2 cut(s) 1017, 3214
Eco24I GRGCYC 1 cut(s) 2742
Eco31I GGTCTC 1 cut(s) 265
Eco47I GGWCC 7 cut(s) 4, 1466, 1706, 1805, 1912, 1994, 2236
Eco47III AGCGCT 1 cut(s) 892
Eco53kI GAGCTC 1 cut(s) 2740
Eco57I CTGAAG 5 cut(s) 450, 929, 2526, 2835, 2856
Eco72I CACGTG 2 cut(s) 781, 1712
Eco81I CCTNAGG 1 cut(s) 2439
Eco88I CYCGRG 1 cut(s) 3201
EcoICRI GAGCTC 1 cut(s) 2740
EcoNI CCTNNNNNAGG 2 cut(s) 1461, 2438
EcoO109I RGGNCCY 3 cut(s) 1466, 1912, 1994
EcoRI GAATTC 4 cut(s) 994, 1191, 1525, 2374
EcoRII CCWGG 6 cut(s) 1501, 1741, 1752, 2098, 2135, 2305
EcoT14I CCWWGG 2 cut(s) 1017, 3214
EcoT22I ATGCAT 4 cut(s) 1363, 1430, 1579, 2959
EcoT38I GRGCYC 1 cut(s) 2742
ErhI CCWWGG 2 cut(s) 1017, 3214
FalI AAGNNNNNCTT 4 cut(s) 52, 84, 1603, 1635
FaqI GGGAC 2 cut(s) 1719, 1898
FbaI TGATCA 2 cut(s) 388, 1735
FblI GTMKAC 2 cut(s) 2005, 2113
Fnu4HI GCNGC 7 cut(s) 129, 183, 1320, 2133, 2499, 3233, 3282
FokI GGATG 9 cut(s) 77, 478, 1050, 1796, 2312, 2386, 2390, 2666, 3233
FriOI GRGCYC 1 cut(s) 2742
Fsp4HI GCNGC 7 cut(s) 129, 183, 1320, 2133, 2499, 3233, 3282
GlaI GCGC 2 cut(s) 121, 892
GluI GCNGC 7 cut(s) 129, 183, 1320, 2133, 2499, 3233, 3282
HaeII RGCGCY 1 cut(s) 894
HaeIII GGCC 5 cut(s) 449, 1220, 1752, 2433, 2448
HapII CCGG 4 cut(s) 815, 1703, 2856, 2932
HgaI GACGC 1 cut(s) 126
HhaI GCGC 2 cut(s) 122, 893
Hin6I GCGC 2 cut(s) 120, 891
HinP1I GCGC 2 cut(s) 120, 891
HincII GTYRAC 3 cut(s) 1292, 1660, 1767
HindII GTYRAC 3 cut(s) 1292, 1660, 1767
HindIII AAGCTT 4 cut(s) 934, 2271, 2687, 3344
HpaII CCGG 4 cut(s) 815, 1703, 2856, 2932
HphI GGTGA 5 cut(s) 1681, 1692, 2088, 2303, 2789
Hpy99I CGWCG 1 cut(s) 2340
HpyCH4III ACNGT 8 cut(s) 276, 1072, 1296, 1314, 1655, 1896, 2809, 3104
HpyCH4IV ACGT 8 cut(s) 556, 780, 901, 1711, 2673, 3166, 3326, 3353
HpySE526I ACGT 8 cut(s) 556, 780, 901, 1711, 2673, 3166, 3326, 3353
HspAI GCGC 2 cut(s) 120, 891
Ksp22I TGATCA 2 cut(s) 388, 1735
LmnI GCTCC 3 cut(s) 97, 660, 3180
Lsp1109I GCAGC 7 cut(s) 115, 194, 1331, 2119, 2510, 3244, 3268
MabI ACCWGGT 2 cut(s) 2098, 2305
MaeII ACGT 8 cut(s) 556, 780, 901, 1711, 2673, 3166, 3326, 3353
MaeIII GTNAC 8 cut(s) 232, 754, 1147, 2809, 2875, 3030, 3098, 3187
MfeI CAATTG 2 cut(s) 2121, 2379
MflI RGATCY 4 cut(s) 192, 293, 1093, 1586
MhlI GDGCHC 4 cut(s) 907, 2742, 2946, 3208
MlyI GAGTC 3 cut(s) 1144, 1772, 2134
MmeI TCCRAC 5 cut(s) 331, 1015, 1168, 2605, 3374
Mph1103I ATGCAT 4 cut(s) 1363, 1430, 1579, 2959
MroXI GAANNNNTTC 3 cut(s) 1275, 1618, 3309
MslI CAYNNNNRTG 2 cut(s) 926, 2772
MspCI CTTAAG 2 cut(s) 3070, 3341
MspI CCGG 4 cut(s) 815, 1703, 2856, 2932
MspR9I CCNGG 8 cut(s) 1503, 1704, 1743, 1754, 2100, 2137, 2307, 2856
MunI CAATTG 2 cut(s) 2121, 2379
MvaI CCWGG 6 cut(s) 1503, 1743, 1754, 2100, 2137, 2307
MvnI CGCG 1 cut(s) 120
NciI CCSGG 2 cut(s) 1704, 2856
NcoI CCATGG 1 cut(s) 1017
NlaIV GGNNCC 9 cut(s) 40, 1095, 1707, 1914, 2066, 2237, 2579, 2750, 3207
NmeAIII GCCGAG 2 cut(s) 847, 1183
NmuCI GTSAC 4 cut(s) 1147, 2809, 2875, 3187
NsiI ATGCAT 4 cut(s) 1363, 1430, 1579, 2959
NspI RCATGY 5 cut(s) 346, 1432, 1489, 1721, 2216
PaeI GCATGC 1 cut(s) 346
PagI TCATGA 2 cut(s) 2143, 3141
PciI ACATGT 2 cut(s) 1485, 1717
PdmI GAANNNNTTC 3 cut(s) 1275, 1618, 3309
PflFI GACNNNGTC 1 cut(s) 1655
PflMI CCANNNNNTGG 3 cut(s) 249, 588, 3215
PfoI TCCNGGA 1 cut(s) 1501
PkrI GCNGC 7 cut(s) 130, 184, 1321, 2134, 2500, 3234, 3283
PleI GAGTC 3 cut(s) 1144, 1771, 2134
PmaCI CACGTG 2 cut(s) 781, 1712
PmlI CACGTG 2 cut(s) 781, 1712
PpsI GAGTC 3 cut(s) 1144, 1771, 2134
Ppu21I YACGTR 5 cut(s) 557, 781, 902, 1712, 3327
PpuMI RGGWCCY 3 cut(s) 1466, 1912, 1994
PscI ACATGT 2 cut(s) 1485, 1717
PsiI TTATAA 2 cut(s) 2198, 2258
Psp124BI GAGCTC 1 cut(s) 2742
Psp1406I AACGTT 1 cut(s) 3166
Psp5II RGGWCCY 3 cut(s) 1466, 1912, 1994
Psp6I CCWGG 6 cut(s) 1501, 1741, 1752, 2098, 2135, 2305
PspCI CACGTG 2 cut(s) 781, 1712
PspGI CCWGG 6 cut(s) 1501, 1741, 1752, 2098, 2135, 2305
PspN4I GGNNCC 9 cut(s) 40, 1095, 1707, 1914, 2066, 2237, 2579, 2750, 3207
PspPI GGNCC 8 cut(s) 4, 447, 1466, 1706, 1805, 1912, 1994, 2236
PspPPI RGGWCCY 3 cut(s) 1466, 1912, 1994
PstNI CAGNNNCTG 3 cut(s) 1325, 1997, 2813
PsuI RGATCY 4 cut(s) 192, 293, 1093, 1586
PsyI GACNNNGTC 1 cut(s) 1655
RsaI GTAC 5 cut(s) 398, 424, 1015, 1729, 1970
RsaNI GTAC 5 cut(s) 397, 423, 1014, 1728, 1969
RseI CAYNNNNRTG 2 cut(s) 926, 2772
SacI GAGCTC 1 cut(s) 2742
SatI GCNGC 7 cut(s) 129, 183, 1320, 2133, 2499, 3233, 3282
Sau96I GGNCC 8 cut(s) 4, 447, 1466, 1706, 1805, 1912, 1994, 2236
SchI GAGTC 3 cut(s) 1144, 1772, 2134
ScrFI CCNGG 8 cut(s) 1503, 1704, 1743, 1754, 2100, 2137, 2307, 2856
SduI GDGCHC 4 cut(s) 907, 2742, 2946, 3208
SexAI ACCWGGT 2 cut(s) 2098, 2305
SfcI CTRYAG 3 cut(s) 186, 1809, 2743
SinI GGWCC 7 cut(s) 4, 1466, 1706, 1805, 1912, 1994, 2236
SmiMI CAYNNNNRTG 2 cut(s) 926, 2772
SmlI CTYRAG 5 cut(s) 87, 1643, 2582, 3070, 3341
SmoI CTYRAG 5 cut(s) 87, 1643, 2582, 3070, 3341
SnaBI TACGTA 1 cut(s) 557
SpeI ACTAGT 4 cut(s) 733, 1730, 2108, 3191
SphI GCATGC 1 cut(s) 346
SsiI CCGC 1 cut(s) 2210
SstI GAGCTC 1 cut(s) 2742
StyD4I CCNGG 8 cut(s) 1501, 1702, 1741, 1752, 2098, 2135, 2305, 2854
StyI CCWWGG 2 cut(s) 1017, 3214
TaaI ACNGT 8 cut(s) 276, 1072, 1296, 1314, 1655, 1896, 2809, 3104
TaiI ACGT 8 cut(s) 559, 783, 904, 1714, 2676, 3169, 3329, 3356
TaqI TCGA 4 cut(s) 1226, 1279, 2338, 3115
TatI WGTACW 2 cut(s) 1727, 1968
TseFI GTSAC 4 cut(s) 1147, 2809, 2875, 3187
TseI GCWGC 7 cut(s) 128, 182, 1319, 2132, 2498, 3232, 3281
Tsp45I GTSAC 4 cut(s) 1147, 2809, 2875, 3187
TspGWI ACGGA 1 cut(s) 509
Tth111I GACNNNGTC 1 cut(s) 1655
Van91I CCANNNNNTGG 3 cut(s) 249, 588, 3215
Vha464I CTTAAG 2 cut(s) 3070, 3341
VneI GTGCAC 1 cut(s) 903
VpaK11BI GGWCC 7 cut(s) 4, 1466, 1706, 1805, 1912, 1994, 2236
XagI CCTNNNNNAGG 2 cut(s) 1461, 2438
XbaI TCTAGA 1 cut(s) 1531
XceI RCATGY 5 cut(s) 346, 1432, 1489, 1721, 2216
XmiI GTMKAC 2 cut(s) 2005, 2113
XmnI GAANNNNTTC 3 cut(s) 1275, 1618, 3309
Zsp2I ATGCAT 4 cut(s) 1363, 1430, 1579, 2959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.