FvH4_6g17370

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0010433
Physical Location & Seq
Reverse (-)
11285837 .. 11298624
12788 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g17370.t1

Sequence Viewer

Length: 3501 bp
ATGGACGACCCTCGCAAACATCAGGTCTCGTTGCGGGGCGCGAGCGCCAAGGAAATTACCAGAGACGCCCTGCTCGAGAAGGTCTCTCAGGAACGGGAGCTCCGCCAATACGCCCGACGCGCCACCGCTGCTGCGCTCTTCCTTCAGAGAGTGTGGAGGCGGTATAGGGTGACTAAGACGGTGGCGGTGGAGCTGAGAGAGGAGTGGGAGAAGTGCGTGAAGCAGCAGCAAGCTGGTTTAGTCATCACATCAATTTGGATTTCGAGTCATGTAGTGAGGCCTTTTCTTTTCTTCATTACATGTTTATCGATTCGGAAGAGGAGAATCGAAGCCGCAGAAGTTAGATCTATGAAGTATTGTTTTCAGATGCTACTAGACAGCTTGAATTCAACTGACTCGAGGAAGAACTATTGCACTCTGGCGATTGGAACTATTGAGGAGAGAAGAATTTGGAGTTATCAGGCGTGGAGGCTGATTTCTGTTTGTATGTTTGTTCTTTCGGAATGTGACAAGTCTCGGTCAGGAAGTCAAGACATTGTTGCTCTCACATCCCTGGCAATGCGGCTTGTTGTGGTCTTGACTGATGTCAAGGGTTGGAAGAGTGTTGATGAACATGACTGTCAGATTGCGGATACGGCAGTGAAGGATTTAGTTCGGTTTATGGGGGGTGGTGAAAGTGGTCTTTACTCATCAATTAGAACATACATTAACACATTGGATGCTCCTTTCTCTTTGCGGACAAGAATTAGTGTACCAACAGATGACAGATTTTTGATTACTGCAAGCACAATAACTTTGGCTCTTAGGCCATTCCATGTGTCAAAGTTCGATGTAAATAGCCTTGGTTTGTTGGATGTCCATAATGTTGCTGAGAAGTACTCTGTGTTTCTGCTTACAATTCCTTGGCTTACTCAGCGACTGCCAGCTGTACTTATACCTGCAATGAGGCACAAATCCATTCTCCAACCTTGTTTTCAAACATTGCTGATTTTGAAAGAGAAAATATTAAAGGAGATGCTGGCTGTGGATCAGTCAAAGTTTCATGATTCTTCGAAGGTTATTCCACCAGTTGGTTGGGCTCTTGCAAACATCATATGCCTAGCTACAGGGGGAGAGTATGATTCTGTTGATCCTGGAGGTTTCCATCAAGAGTTGGATTGCGCATCTTATATACATGCTGTTAATACTCTTGCAGAAAACTTATTGTCTAGGCTTGAGAGTGTTGTCCAGGAGAGTCAGGATCTCCAAAGCAATGTTGAAACCAGTGAAAAGCCTAGCTCTACGGTTTCATATGAGAGTGAAATGACCCATGGATCTATTAAGTTGTCGTTCTTGGATATGCTTAGGCCTGTTTCTCACCAGTGGCACCTTACAGATCTTTTGACAATAGTGAACACTCAAGGGTCTGAGACTATGACACCAGAAAGACAGGAATATTCAGGGAAGTTGGAGTTGCTGGATATTGTGCATTTTTATTCTTTCATGCTAAGAATGTTTTCATATTTGAATCCAAGGGTTGGCTCGCTGCCTGTCCTTAACATGCTATCGTTTACTCCTGGATTTCTTGTGAGTCTATGGGGAGCATTGGAAACTTATCTCTTTCCTAGGATTGTTTGCAGTGATCGTAAACCCTATGATAACATTAGTAAAACTTCTGGTAGTGGAAAGGATGGGAATTCTGGAAAAAGGAAAACGCATGGCAATAATGATGGAGGTAAAAAGTGGGTCAGTGTCCTTCATAAAATTACTGGTAAATCACAATCGGGGATTGGTCACACAGACTTGTGTGCTAATGAACCCAAGACTAGACTTATTGACAAAGAGGATTCTTCTGATGTTTGGGATGTAGAACCTGTGAGACCTGGTCCACAAGGTATTTCAAGAGACATGTCATGTATGCTTCATCTATTCTGTGCAAGCTATTCACACCTGCTTTTGATTCTCGATGACATAGAATTTTATGAAAAACAGGTACCATTTACACTGGAGCAACAACGGCAAATTGCATCTGTTCTCAATACATTGGTTTATAATGGGTTTTCTCAAAGTATTGGACAGGAGGGTAGACCCCTTATGGAGTCTGCTGTCAGATGCTTGCATTTAATATATGAAAGGGATTGCAGGCACCAGTTCTGCCCTCCTGTTTTGTGGCTTTCACCTGCTCGAAAGAACCGCCCACCAATTGCTGTAGCTGCCAGAACTCATGAAGTGTTGTCAGCTAATCAAAGATCAGATGATCCTCTGGCTGTGCAAAGTATGGGATCTGTTATAACTACAACTCCACATGTATTTCCATTTGAAGAAAGAGTGGAAATGTTTAGAGAGTTTATCAAGATGGACAAAGCCTCCCGAATAATGGCTGGTGAGGTTGCTGGCCCTAGTTCACGATCAGTTGACATTGTAGTTCGTCGTGGTCATATTTTTGAAGATGGATTTCGGCAGTTAAATTCCCTGGGATCAAGATTGAAGTCATCCATCCATGTTTCATTTGTCAGTGAATGTGGTCTTCCAGAGGCTGGCCTCGACTATGGTGGATTATCTAAAGAGTTTTTGACCGATATATCAAAAGCTGCATTCGCTCCCGAGTATGGACTATTCTCCCAGACATCAACCTCGGCCAGACTTCTAATTCCTAATCCATCTGCAAGATATCTAGAGAATGGTATCCAGATGATTGAGTTCCTTGGAAGAGTTGTTGGGAAAGCTCTCTATGAAGGAATACTTCTAGATTACTCATTTTCTCATGTTTTTGTACACAAATTGTTGGGTCGATATAGCTTCCTTGATGAGCTTTCAACACTAGACCCAGAGATCTACAGGAATCTCATGTATGTTAAGCATTATGATGGTGATGTTGAGGAACTCTGTCTGGACTTTACAGTTACTGAAGAGTCATTTGGAAAACGACATGTTATTGAGCTAAAGCCTGGTGGAAAGGATGTTACTGTGACAAGCAAGAACAAAATGCAGTATATCCATGCAATTGCTGATTATAAGCTTAACCGACAGATGTTTCTTTTTTCAAATGCATTCTATAGAGGGTTGATTGATCTCATATCACCATCTTGGCTGAAATTATTTAATGCAGGGGAATTTAATCAGTTGCTTTCAGGTGGAAACCATGACATTGATGTTGATGACTTGAGAAAGAACACAAGATATACTGGTGGTTACTCTGAAGGGAACCGGACGATTAAAATCTTTTGGGAGGTGATTAGTGGATTTGAACCTACAGAACGGTGTATGCTTCTGAAATTCGTGACCAGTTGTTCTCGAGCTCCATTACTTGGATTTAAGCACTTGCAACCAACTTTTACAATCCATAAGGTTGCATGCGATATCCCTCTGTGGGCAACAATGAGAGGACAGGATGTGGAGCGGCTTCCATCAGCCTCAACTTGCTACAATACTCTGAAGCTTCCAACATACAAGCGTCCAAGCACGTTGAGAGAAAAACTACTATATGCAATCAGTTCAAATGCAGGTTTTGAACTTTCTTGA

Protein Analysis

1167

Amino Acids

131.9

Weight (kDa)

8.16

Isoelectric Point (pI)

45.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HECT PF00632 853 - 1166 1.9e-94 HECT-domain (ubiquitin-transferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2031, 2270, 2994
AarI CACCTGC 2 cut(s) 1938, 2167
Acc16I TGCGCA 1 cut(s) 1162
Acc36I ACCTGC 4 cut(s) 946, 1938, 2167, 3473
Acc65I GGTACC 1 cut(s) 1972
AccB1I GGYRCC 3 cut(s) 1365, 1972, 2124
AccB7I CCANNNNNTGG 4 cut(s) 1070, 1517, 2516, 3287
AccBSI CCGCTC 1 cut(s) 3379
AccI GTMKAC 1 cut(s) 2065
AccII CGCG 2 cut(s) 41, 120
AclWI GGATC 7 cut(s) 1035, 1124, 1248, 1321, 2231, 2269, 2464
AcoI YGGCCR 1 cut(s) 2616
AcsI RAATTY 7 cut(s) 385, 447, 1675, 1955, 2446, 3092, 3254
AcuI CTGAAG 4 cut(s) 128, 2907, 3198, 3434
AcyI GRCGYC 1 cut(s) 66
AfaI GTAC 5 cut(s) 753, 878, 930, 1974, 2754
AfiI CCNNNNNNNGG 7 cut(s) 1070, 1517, 2356, 2516, 2588, 3287, 3349
AflIII ACRYGT 4 cut(s) 299, 1887, 2284, 2908
AjnI CCWGG 7 cut(s) 552, 1132, 1227, 1555, 1861, 2451, 2926
AjuI GAANNNNNNNTTGG 4 cut(s) 957, 989, 2880, 2912
AloI GAACNNNNNNTCC 2 cut(s) 84, 116
Alw21I GWGCWC 2 cut(s) 102, 3280
Alw26I GTCTC 7 cut(s) 31, 57, 88, 519, 1403, 1852, 1878
AlwI GGATC 7 cut(s) 1035, 1124, 1248, 1321, 2231, 2269, 2464
AlwNI CAGNNNCTG 3 cut(s) 919, 2516, 2885
Ama87I CYCGRG 4 cut(s) 74, 397, 2582, 3273
AoxI GGCC 6 cut(s) 278, 806, 1346, 2374, 2518, 2616
ApeKI GCWGC 7 cut(s) 128, 131, 223, 226, 1525, 2192, 2570
ApoI RAATTY 7 cut(s) 385, 447, 1675, 1955, 2446, 3092, 3254
ArsI GACNNNNNNTTYG 2 cut(s) 9, 41
Asp700I GAANNNNTTC 1 cut(s) 1495
Asp718I GGTACC 1 cut(s) 1972
AspA2I CCTAGG 1 cut(s) 1604
AspLEI GCGC 5 cut(s) 41, 47, 122, 136, 1163
AspS9I GGNCC 2 cut(s) 1865, 2375
AsuHPI GGTGA 8 cut(s) 181, 683, 1349, 2148, 2375, 2861, 3051, 3223
AsuII TTCGAA 1 cut(s) 1052
AvaI CYCGRG 4 cut(s) 74, 397, 2582, 3273
AvaII GGWCC 1 cut(s) 1865
AvrII CCTAGG 1 cut(s) 1604
BaeI ACNNNNGTAYC 2 cut(s) 624, 657
BanI GGYRCC 3 cut(s) 1365, 1972, 2124
BanII GRGCYC 3 cut(s) 102, 1081, 3280
BbsI GAAGAC 1 cut(s) 2498
Bbv12I GWGCWC 2 cut(s) 102, 3280
BbvI GCAGC 7 cut(s) 115, 118, 235, 238, 1512, 2179, 2557
BceAI ACGGC 2 cut(s) 651, 2012
BciT130I CCWGG 7 cut(s) 554, 1134, 1229, 1557, 1863, 2453, 2928
BciVI GTATCC 2 cut(s) 625, 2675
BcoDI GTCTC 7 cut(s) 31, 57, 88, 519, 1403, 1852, 1878
BfmI CTRYAG 5 cut(s) 1104, 2187, 2815, 3034, 3231
BfoI RGCGCY 1 cut(s) 48
BfuAI ACCTGC 4 cut(s) 946, 1938, 2167, 3473
BfuI GTATCC 2 cut(s) 625, 2675
BglII AGATCT 3 cut(s) 344, 1375, 2811
BlnI CCTAGG 1 cut(s) 1604
BmcAI AGTACT 1 cut(s) 878
Bme1390I CCNGG 7 cut(s) 554, 1134, 1229, 1557, 1863, 2453, 2928
Bme18I GGWCC 1 cut(s) 1865
BmeT110I CYCGRG 4 cut(s) 74, 397, 2582, 3273
BmgT120I GGNCC 2 cut(s) 1865, 2375
BmiI GGNNCC 4 cut(s) 1367, 1974, 2126, 3185
BmrFI CCNGG 7 cut(s) 554, 1134, 1229, 1557, 1863, 2453, 2928
BmsI GCATC 6 cut(s) 357, 709, 1005, 1172, 2015, 2081
BoxI GACNNNNGTC 1 cut(s) 584
BpiI GAAGAC 1 cut(s) 2498
BplI GAGNNNNNCTC 4 cut(s) 68, 100, 863, 895
BpmI CTGGAG 2 cut(s) 1155, 2006
Bpu10I CCTNAGC 1 cut(s) 1343
Bpu14I TTCGAA 1 cut(s) 1052
BpuEI CTTGAG 3 cut(s) 1235, 1383, 3163
Bsa29I ATCGAT 1 cut(s) 308
BsaBI GATNNNNATC 1 cut(s) 3197
BsaHI GRCGYC 1 cut(s) 66
BsaI GGTCTC 3 cut(s) 31, 88, 1852
BsaWI WCCGGW 1 cut(s) 3186
BsaXI ACNNNNNCTCC 6 cut(s) 84, 114, 2263, 2293, 2330, 2360
Bsc4I CCNNNNNNNGG 7 cut(s) 1070, 1517, 2356, 2516, 2588, 3287, 3349
Bse1I ACTGG 8 cut(s) 1067, 1263, 1360, 1753, 1989, 2128, 3169, 3264
Bse3DI GCAATG 4 cut(s) 564, 948, 980, 1258
Bse8I GATNNNNATC 1 cut(s) 3197
BseBI CCWGG 7 cut(s) 554, 1134, 1229, 1557, 1863, 2453, 2928
BseCI ATCGAT 1 cut(s) 308
BseGI GGATG 9 cut(s) 548, 724, 859, 1675, 1849, 2471, 2475, 2944, 3376
BseJI GATNNNNATC 1 cut(s) 3197
BseLI CCNNNNNNNGG 7 cut(s) 1070, 1517, 2356, 2516, 2588, 3287, 3349
BseMI GCAATG 4 cut(s) 564, 948, 980, 1258
BseMII CTCAG 5 cut(s) 101, 185, 861, 926, 1398
BseNI ACTGG 8 cut(s) 1067, 1263, 1360, 1753, 1989, 2128, 3169, 3264
BseRI GAGGAG 3 cut(s) 215, 334, 452
BseXI GCAGC 7 cut(s) 115, 118, 235, 238, 1512, 2179, 2557
Bsh1236I CGCG 2 cut(s) 41, 120
BshFI GGCC 6 cut(s) 280, 808, 1348, 2376, 2520, 2618
BshNI GGYRCC 3 cut(s) 1365, 1972, 2124
BshVI ATCGAT 1 cut(s) 308
BsiHKAI GWGCWC 2 cut(s) 102, 3280
BsiHKCI CYCGRG 4 cut(s) 74, 397, 2582, 3273
BsiSI CCGG 1 cut(s) 3187
BslI CCNNNNNNNGG 7 cut(s) 1070, 1517, 2356, 2516, 2588, 3287, 3349
BsmAI GTCTC 7 cut(s) 31, 57, 88, 519, 1403, 1852, 1878
BsmBI CGTCTC 1 cut(s) 57
BsmI GAATGC 2 cut(s) 2573, 3029
BsnI GGCC 6 cut(s) 280, 808, 1348, 2376, 2520, 2618
Bso31I GGTCTC 3 cut(s) 31, 88, 1852
BsoBI CYCGRG 4 cut(s) 74, 397, 2582, 3273
Bsp119I TTCGAA 1 cut(s) 1052
Bsp1286I GDGCHC 3 cut(s) 102, 1081, 3280
Bsp1407I TGTACA 1 cut(s) 2752
Bsp19I CCATGG 1 cut(s) 1309
BspANI GGCC 6 cut(s) 280, 808, 1348, 2376, 2520, 2618
BspCNI CTCAG 5 cut(s) 100, 186, 862, 925, 1399
BspDI ATCGAT 1 cut(s) 308
BspFNI CGCG 2 cut(s) 41, 120
BspHI TCATGA 2 cut(s) 1042, 2203
BspLI GGNNCC 4 cut(s) 1367, 1974, 2126, 3185
BspMI ACCTGC 4 cut(s) 946, 1938, 2167, 3473
BspPI GGATC 7 cut(s) 1035, 1124, 1248, 1321, 2231, 2269, 2464
BspQI GCTCTTC 1 cut(s) 143
BspT104I TTCGAA 1 cut(s) 1052
BspT107I GGYRCC 3 cut(s) 1365, 1972, 2124
BspTNI GGTCTC 3 cut(s) 31, 88, 1852
BsrBI CCGCTC 1 cut(s) 3379
BsrDI GCAATG 4 cut(s) 564, 948, 980, 1258
BsrGI TGTACA 1 cut(s) 2752
BsrI ACTGG 8 cut(s) 1067, 1263, 1360, 1753, 1989, 2128, 3169, 3264
BssNI GRCGYC 1 cut(s) 66
BssT1I CCWWGG 7 cut(s) 48, 841, 902, 1309, 1511, 1604, 2683
Bst2UI CCWGG 7 cut(s) 554, 1134, 1229, 1557, 1863, 2453, 2928
Bst4CI ACNGT 6 cut(s) 181, 620, 1285, 2881, 2947, 3240
Bst6I CTCTTC 5 cut(s) 143, 311, 593, 2683, 2883
BstACI GRCGYC 1 cut(s) 66
BstAUI TGTACA 1 cut(s) 2752
BstBI TTCGAA 1 cut(s) 1052
BstDEI CTNAG 9 cut(s) 87, 174, 194, 803, 870, 912, 1343, 1407, 1487
BstDSI CCRYGG 1 cut(s) 1309
BstF5I GGATG 9 cut(s) 548, 724, 859, 1675, 1849, 2471, 2475, 2944, 3376
BstFNI CGCG 2 cut(s) 41, 120
BstH2I RGCGCY 1 cut(s) 48
BstHHI GCGC 5 cut(s) 41, 47, 122, 136, 1163
BstMAI GTCTC 7 cut(s) 31, 57, 88, 519, 1403, 1852, 1878
BstMWI GCNNNNNNNGC 7 cut(s) 119, 128, 635, 913, 1996, 2192, 2576
BstNI CCWGG 7 cut(s) 554, 1134, 1229, 1557, 1863, 2453, 2928
BstNSI RCATGY 7 cut(s) 303, 1178, 1543, 1891, 2288, 2912, 3336
BstPAI GACNNNNGTC 1 cut(s) 584
BstSCI CCNGG 7 cut(s) 552, 1132, 1227, 1555, 1861, 2451, 2926
BstSFI CTRYAG 5 cut(s) 1104, 2187, 2815, 3034, 3231
BstUI CGCG 2 cut(s) 41, 120
BstV1I GCAGC 7 cut(s) 115, 118, 235, 238, 1512, 2179, 2557
BstV2I GAAGAC 1 cut(s) 2498
BstX2I RGATCY 6 cut(s) 344, 1240, 1313, 1375, 2261, 2811
BstXI CCANNNNNNTGG 1 cut(s) 1074
BstYI RGATCY 6 cut(s) 344, 1240, 1313, 1375, 2261, 2811
Bsu15I ATCGAT 1 cut(s) 308
BsuI GTATCC 2 cut(s) 625, 2675
BsuRI GGCC 6 cut(s) 280, 808, 1348, 2376, 2520, 2618
BsuTUI ATCGAT 1 cut(s) 308
BtgI CCRYGG 1 cut(s) 1309
BtsCI GGATG 9 cut(s) 548, 724, 859, 1675, 1849, 2471, 2475, 2944, 3376
BtsI GCAGTG 2 cut(s) 645, 1624
BtsIMutI CAGTG 7 cut(s) 645, 1270, 1367, 1624, 1735, 1982, 2500
BveI ACCTGC 4 cut(s) 946, 1938, 2167, 3473
CaiI CAGNNNCTG 3 cut(s) 919, 2516, 2885
CciI TCATGA 2 cut(s) 1042, 2203
CfoI GCGC 5 cut(s) 41, 47, 122, 136, 1163
Cfr13I GGNCC 2 cut(s) 1865, 2375
ClaI ATCGAT 1 cut(s) 308
CseI GACGC 3 cut(s) 74, 126, 3422
CsiI ACCWGGT 1 cut(s) 1861
Csp6I GTAC 5 cut(s) 752, 877, 929, 1973, 2753
CviQI GTAC 5 cut(s) 752, 877, 929, 1973, 2753
DdeI CTNAG 9 cut(s) 87, 174, 194, 803, 870, 912, 1343, 1407, 1487
EaeI YGGCCR 1 cut(s) 2616
Eam1104I CTCTTC 5 cut(s) 143, 311, 593, 2683, 2883
EarI CTCTTC 5 cut(s) 143, 311, 593, 2683, 2883
EciI GGCGGA 1 cut(s) 92
Ecl136II GAGCTC 2 cut(s) 100, 3278
Eco130I CCWWGG 7 cut(s) 48, 841, 902, 1309, 1511, 1604, 2683
Eco147I AGGCCT 2 cut(s) 280, 1348
Eco24I GRGCYC 3 cut(s) 102, 1081, 3280
Eco31I GGTCTC 3 cut(s) 31, 88, 1852
Eco32I GATATC 2 cut(s) 2651, 3340
Eco47I GGWCC 1 cut(s) 1865
Eco53kI GAGCTC 2 cut(s) 100, 3278
Eco57I CTGAAG 4 cut(s) 128, 2907, 3198, 3434
Eco88I CYCGRG 4 cut(s) 74, 397, 2582, 3273
EcoICRI GAGCTC 2 cut(s) 100, 3278
EcoRI GAATTC 2 cut(s) 385, 1675
EcoRII CCWGG 7 cut(s) 552, 1132, 1227, 1555, 1861, 2451, 2926
EcoRV GATATC 2 cut(s) 2651, 3340
EcoT14I CCWWGG 7 cut(s) 48, 841, 902, 1309, 1511, 1604, 2683
EcoT22I ATGCAT 1 cut(s) 3031
EcoT38I GRGCYC 3 cut(s) 102, 1081, 3280
ErhI CCWWGG 7 cut(s) 48, 841, 902, 1309, 1511, 1604, 2683
Esp3I CGTCTC 1 cut(s) 57
FalI AAGNNNNNCTT 2 cut(s) 2706, 2738
FauI CCCGC 1 cut(s) 27
FauNDI CATATG 2 cut(s) 1094, 1291
FblI GTMKAC 1 cut(s) 2065
FokI GGATG 9 cut(s) 535, 731, 866, 1682, 1856, 2458, 2462, 2951, 3383
FriOI GRGCYC 3 cut(s) 102, 1081, 3280
FspI TGCGCA 1 cut(s) 1162
GlaI GCGC 5 cut(s) 40, 46, 121, 135, 1162
GsuI CTGGAG 2 cut(s) 1155, 2006
HaeII RGCGCY 1 cut(s) 48
HaeIII GGCC 6 cut(s) 280, 808, 1348, 2376, 2520, 2618
HapII CCGG 1 cut(s) 3187
HgaI GACGC 3 cut(s) 74, 126, 3422
HhaI GCGC 5 cut(s) 41, 47, 122, 136, 1163
Hin1I GRCGYC 1 cut(s) 66
Hin6I GCGC 5 cut(s) 39, 45, 120, 134, 1161
HinP1I GCGC 5 cut(s) 39, 45, 120, 134, 1161
HincII GTYRAC 1 cut(s) 2395
HindII GTYRAC 1 cut(s) 2395
HindIII AAGCTT 2 cut(s) 2996, 3416
HpaII CCGG 1 cut(s) 3187
HphI GGTGA 8 cut(s) 181, 683, 1349, 2148, 2375, 2861, 3051, 3223
Hpy166II GTNNAC 9 cut(s) 752, 1393, 1551, 1628, 1868, 2066, 2384, 2395, 2755
Hpy8I GTNNAC 9 cut(s) 752, 1393, 1551, 1628, 1868, 2066, 2384, 2395, 2755
Hpy99I CGWCG 2 cut(s) 120, 2412
HpyAV CCTTC 7 cut(s) 73, 152, 637, 1048, 1745, 2708, 3173
HpyCH4III ACNGT 6 cut(s) 181, 620, 1285, 2881, 2947, 3240
HpyCH4IV ACGT 1 cut(s) 3443
HpyF10VI GCNNNNNNNGC 7 cut(s) 119, 128, 635, 913, 1996, 2192, 2576
HpyF3I CTNAG 9 cut(s) 87, 174, 194, 803, 870, 912, 1343, 1407, 1487
HpySE526I ACGT 1 cut(s) 3443
Hsp92I GRCGYC 1 cut(s) 66
HspAI GCGC 5 cut(s) 39, 45, 120, 134, 1161
KpnI GGTACC 1 cut(s) 1976
LguI GCTCTTC 1 cut(s) 143
LmnI GCTCC 9 cut(s) 97, 105, 190, 727, 1580, 1987, 2584, 3283, 3376
Lsp1109I GCAGC 7 cut(s) 115, 118, 235, 238, 1512, 2179, 2557
LweI GCATC 6 cut(s) 357, 709, 1005, 1172, 2015, 2081
MabI ACCWGGT 1 cut(s) 1861
MaeII ACGT 1 cut(s) 3443
MaeIII GTNAC 8 cut(s) 169, 506, 1772, 2881, 2941, 2947, 3170, 3259
MbiI CCGCTC 1 cut(s) 3379
MfeI CAATTG 2 cut(s) 2181, 2982
MflI RGATCY 6 cut(s) 344, 1240, 1313, 1375, 2261, 2811
MhlI GDGCHC 3 cut(s) 102, 1081, 3280
MlyI GAGTC 6 cut(s) 274, 389, 1243, 1579, 2087, 2900
MmeI TCCRAC 6 cut(s) 575, 831, 988, 1134, 1428, 3446
Mph1103I ATGCAT 1 cut(s) 3031
MroXI GAANNNNTTC 1 cut(s) 1495
MslI CAYNNNNRTG 1 cut(s) 2844
MspA1I CMGCKG 2 cut(s) 128, 926
MspI CCGG 1 cut(s) 3187
MspR9I CCNGG 7 cut(s) 554, 1134, 1229, 1557, 1863, 2453, 2928
MunI CAATTG 2 cut(s) 2181, 2982
Mva1269I GAATGC 2 cut(s) 2573, 3029
MvaI CCWGG 7 cut(s) 554, 1134, 1229, 1557, 1863, 2453, 2928
MvnI CGCG 2 cut(s) 41, 120
MwoI GCNNNNNNNGC 7 cut(s) 119, 128, 635, 913, 1996, 2192, 2576
NcoI CCATGG 1 cut(s) 1309
NdeI CATATG 2 cut(s) 1094, 1291
NlaIV GGNNCC 4 cut(s) 1367, 1974, 2126, 3185
NmeAIII GCCGAG 1 cut(s) 2594
NmuCI GTSAC 5 cut(s) 169, 506, 1772, 2947, 3259
NsbI TGCGCA 1 cut(s) 1162
NsiI ATGCAT 1 cut(s) 3031
NspI RCATGY 7 cut(s) 303, 1178, 1543, 1891, 2288, 2912, 3336
NspV TTCGAA 1 cut(s) 1052
PaeI GCATGC 1 cut(s) 3336
PaeR7I CTCGAG 3 cut(s) 74, 397, 3273
PagI TCATGA 2 cut(s) 1042, 2203
PaqCI CACCTGC 2 cut(s) 1938, 2167
PasI CCCWGGG 1 cut(s) 2452
PceI AGGCCT 2 cut(s) 280, 1348
PciI ACATGT 4 cut(s) 299, 1887, 2284, 2908
PciSI GCTCTTC 1 cut(s) 143
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PctI GAATGC 2 cut(s) 2573, 3029
PdmI GAANNNNTTC 1 cut(s) 1495
PfeI GAWTC 8 cut(s) 310, 324, 1046, 1121, 1507, 1826, 1939, 2821
PflFI GACNNNGTC 1 cut(s) 1863
PflMI CCANNNNNTGG 4 cut(s) 1070, 1517, 2516, 3287
PfoI TCCNGGA 3 cut(s) 1132, 1227, 1555
PleI GAGTC 6 cut(s) 273, 389, 1242, 1578, 2086, 2899
PpsI GAGTC 6 cut(s) 273, 389, 1242, 1578, 2086, 2899
PscI ACATGT 4 cut(s) 299, 1887, 2284, 2908
PshAI GACNNNNGTC 1 cut(s) 584
PsiI TTATAA 3 cut(s) 2031, 2270, 2994
Psp124BI GAGCTC 2 cut(s) 102, 3280
Psp6I CCWGG 7 cut(s) 552, 1132, 1227, 1555, 1861, 2451, 2926
PspGI CCWGG 7 cut(s) 552, 1132, 1227, 1555, 1861, 2451, 2926
PspN4I GGNNCC 4 cut(s) 1367, 1974, 2126, 3185
PspPI GGNCC 2 cut(s) 1865, 2375
PspXI VCTCGAGB 1 cut(s) 397
PstNI CAGNNNCTG 3 cut(s) 919, 2516, 2885
PsuI RGATCY 6 cut(s) 344, 1240, 1313, 1375, 2261, 2811
PsyI GACNNNGTC 1 cut(s) 1863
PvuII CAGCTG 1 cut(s) 926
RsaI GTAC 5 cut(s) 753, 878, 930, 1974, 2754
RsaNI GTAC 5 cut(s) 752, 877, 929, 1973, 2753
RseI CAYNNNNRTG 1 cut(s) 2844
SacI GAGCTC 2 cut(s) 102, 3280
SapI GCTCTTC 1 cut(s) 143
Sau96I GGNCC 2 cut(s) 1865, 2375
ScaI AGTACT 1 cut(s) 878
SchI GAGTC 6 cut(s) 274, 389, 1243, 1579, 2087, 2900
ScrFI CCNGG 7 cut(s) 554, 1134, 1229, 1557, 1863, 2453, 2928
SduI GDGCHC 3 cut(s) 102, 1081, 3280
SexAI ACCWGGT 1 cut(s) 1861
SfaNI GCATC 6 cut(s) 357, 709, 1005, 1172, 2015, 2081
SfcI CTRYAG 5 cut(s) 1104, 2187, 2815, 3034, 3231
Sfr274I CTCGAG 3 cut(s) 74, 397, 3273
SfuI TTCGAA 1 cut(s) 1052
SinI GGWCC 1 cut(s) 1865
SlaI CTCGAG 3 cut(s) 74, 397, 3273
SmiMI CAYNNNNRTG 1 cut(s) 2844
SmlI CTYRAG 6 cut(s) 74, 397, 1214, 1398, 3142, 3273
SmoI CTYRAG 6 cut(s) 74, 397, 1214, 1398, 3142, 3273
SphI GCATGC 1 cut(s) 3336
SseBI AGGCCT 2 cut(s) 280, 1348
SspI AATATT 2 cut(s) 1005, 1436
SstI GAGCTC 2 cut(s) 102, 3280
StuI AGGCCT 2 cut(s) 280, 1348
StyD4I CCNGG 7 cut(s) 552, 1132, 1227, 1555, 1861, 2451, 2926
StyI CCWWGG 7 cut(s) 48, 841, 902, 1309, 1511, 1604, 2683
TaaI ACNGT 6 cut(s) 181, 620, 1285, 2881, 2947, 3240
TaiI ACGT 1 cut(s) 3446
TaqII GACCGA 2 cut(s) 507, 2570
TatI WGTACW 3 cut(s) 876, 928, 2752
TauI GCSGC 3 cut(s) 335, 565, 3382
TfiI GAWTC 8 cut(s) 310, 324, 1046, 1121, 1507, 1826, 1939, 2821
TscAI CASTG 7 cut(s) 645, 1270, 1367, 1624, 1735, 1989, 2500
TseFI GTSAC 5 cut(s) 169, 506, 1772, 2947, 3259
TseI GCWGC 7 cut(s) 128, 131, 223, 226, 1525, 2192, 2570
Tsp45I GTSAC 5 cut(s) 169, 506, 1772, 2947, 3259
TspRI CASTG 7 cut(s) 645, 1270, 1367, 1624, 1735, 1989, 2500
Tth111I GACNNNGTC 1 cut(s) 1863
Van91I CCANNNNNTGG 4 cut(s) 1070, 1517, 2516, 3287
VpaK11BI GGWCC 1 cut(s) 1865
XapI RAATTY 7 cut(s) 385, 447, 1675, 1955, 2446, 3092, 3254
XbaI TCTAGA 2 cut(s) 2653, 2725
XceI RCATGY 7 cut(s) 303, 1178, 1543, 1891, 2288, 2912, 3336
XcmI CCANNNNNNNNNTGG 1 cut(s) 1071
XhoI CTCGAG 3 cut(s) 74, 397, 3273
XmaJI CCTAGG 1 cut(s) 1604
XmiI GTMKAC 1 cut(s) 2065
XmnI GAANNNNTTC 1 cut(s) 1495
ZrmI AGTACT 1 cut(s) 878
Zsp2I ATGCAT 1 cut(s) 3031
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.