RLG00000024169

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0010433
Physical Location & Seq
Forward (+)
32725678 .. 32736040
10363 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024169

Sequence Viewer

Length: 3399 bp
ATGGATGATCCTCGCAAGCATCAGGTCTCGTTGAGGGGCGCGAGCGCCAAGGAAATTACCAGAGACGCCCTGCTCGAGAAGGTCTCTCAGGAACGAGAGCTTCGCCAATATGCCAGACGCGCCACCGCTGCTGCCCTCTTCCTTCAGAGAGTGTGGAGGCGCTATAGGGTGACTAAGATGGTGGCTTTGGAGCTCAGAGAGGAGTGGGAGAAGTGTGTGAAGCAGAAGCGAGCTGGTTTAGTCATCACTGCCACCTGGATTTCAAGTCATGTAGTGAGGCCTTTTCTTTTCTTCATTACATGTCTATCGATTCGGAAGAGGAGGATTGAAGCCACAGAAGTTGACTCTATGAAGAGTTGCTTTCAAATGCTACTAGACAGCATGAATTCCACTGACTCGAAGAAGAGCTATTGCTCTCTGGCAATTGGAACTCTCGAGGAGAGAAGAATCTGGGGCTATCAGACGCGGAAGATGATTTCTCTTTGCATGTTTGTTCTTTCGGAATGTGACAAGTCTCGCCCAGGAAGTGAAGACATTGTTGCTCTTACGTCCTTAGCAATGCGGCTTGTTGTTGTCTTAACTGATGTCAAAGGGTGGAAGAGTATTGATGAACATGACTGTCAGATTGCGGATGCAGCAGTGAAGGATTTAGTTCGGTTTATGGGGAGTGGTGAAAGTGGTCTTTACTCATCGATTAGAATATACATTAACACATTGGATGCTCCTTGCTCGTCACGGACAAAAAGTAGTGTACAAACAGATGACAGATTTTTGATTACTGCAAGCACAATAACTTTGGCTCTCAGGCCATTTCATGCGTCAAAGTCCGATCTAAATGGCCTTGGCTTGTTGGATGTCCATAATGTTGCTGAGAAGTACTCTGTGTTTCTGCTTACAATTCCTTGGCTTACTCAGCGTCTGCCAGCTGTACTTCTACCTGCAATGAGGCACAAATCCATTCTCCAACCTTGTTTTCAAACATTACTGGTGATGGCAGATTTTGAAAGAGAAAATATTAAAGGAGATGCTGGCCGTGGATCAGTCAAAGTTTCATGTTTCCCCCAAGAAAACTTATTGTCTAGGCTTGAGAATGTTGACTCTGTCCAGGATAATCAGGATCTCCAAAGTGATGTTGAAACCAGTGAAAAGCCAAGCTGTACAGTTTCATGTGAGACTGAAATGACCCATGGATCTATTAAGTTGTCGTTCTTGGATATGCTTAGGCCTGTTTCTCAGCAGTGGCACCTTACAGATCTTTTGGCAATAGTGAATAAACTTGGTCACACTCAAGGGTCTGAGACTATGACGCCAAAAAGACTGGAATATTCAGGGACGTTGGAATTGCTGGATATTGTGCATTTTTATTCTTTCATGCTAAGAATGTTTTCATTCTTGAATCCAAGGGTTGGCTCATTGCCTGTCCTTAACATGCTATCGTTTACTCCTGGATTTCTTGTAAGTCTATGGGGAGCGTTGGAAACCTGTCTTTTTCCTAGTTATGTTTGCAGTGGTCATAAACCCTATGATATCATTAGTAAAACTTCTGGTAGTGGAAAAGATGGGAATTTTGGAAAAAGGAAAAAGCATGGCAATAATGATGGAGGTAATAAGTGGGTCAGTGTCCTTCATAAAATTACGGGTAAATCACGAGCGGAGATTGATCATACAGATTTGCGTGGTAATCAACCCCAGCCTAGACTTGTTGACAAAGAGGATTCTTCTGATGTTTGGGATATAGAACCTGTGAGGCCTGGTCCTCAAGGTATTTCAAGAGACATGTCATGTATGCTTCATCTATTCTGTGCAAGCTATTCACACCTGCTTTTGATTCTTGATGACATAGAGTTTTATGAAAAACAGGTACCATTTACACTGGAGCAACAACGGCAAATTGCTTCTGTTCTCAATACATTGGTTTATAATGGGTTTTCTCAAAGTATTGGACAGGAGGGTAGACCCCTTATGGAGTCTGCTGTCAGATGCCTGCATTTAATATATGAAAGGGATTGCAGGCACCAGTTCTGCCCTCCTGTTTTGTGGCTTTCACCTGCTAGAAAGAACCGCCCACCAATTGCGGTAGCTGCCAGAACTCATGAAGTTTGGTCAGCTAATCAAAGATCAGATGATTCTCTGGCTGTGCAAAGTATAGGCTCTGTTATAACTACAACTCCGCATGTATTTCCATTTGAAGAAAGAGTGGAAATGTTTAGAGAGTTTATCAAGATGGACAAAGCCTCCCGGAAAATGGCTGGTGAGGTTTCTGGCCCTAGTTCACGATCAGTTGAGATAGTAGTTCGTCGTGGTCATATTGTTGAAGATGGATTTCGGCAGTTAAACTCCCTGGGATCAAGATTAAAGTCATCCATCCATGTTTCATTTGTCAGTGAATGTGGCCTTCCAGAGGCTGGCCTCGACTATGGTGGATTATCTAAAGAGTTTTTGACCGATATATCAAAAGCTGCATTCGCTCCTGAGTATGGGCTATTCTCCCAGACCCCAACCTCAGCCAGGCTTCTAATTCCTAATCCATCTGCAAGATATCTAGAGAATGGTATCCAGATGATTGAGTTCCTTGGAAGAGTTGTCGGGAAAGCTCTCTATGAAGGAATACTGCTAGATTACTCATTTTCTCATGTTTTTGTGCAAAAACTGTTGGGTCGATATAGCTTCCTCGATGAACTATCAACACTTGATCCAGAGATTTACAGGAATCTCATGTATGTTAAGCATTATGATGGTGATGTTGAGGAACTGTGTCTGGACTTTACGGTAACTGAAGAGTCATTTGGAAAACGACATGTTATTGAGCTCAAGCCTGGTGGAAAGGATGTTACTGTGACAAGCAAGAACAAAATGCAGTACATCCATGCAATTGCTGATTATAAGCTTAACCGACAGATGTTCCTTTTTTCAAATGCATTCTATAGAGGGTTAATTGATCTCATATCACCATCTTGGCTAAAATTATTTAATGCAGGGGAATTTAATCAGTTGCTTTCAGGTGGAAACCATGACATTGATGTTGATGACTTGAGAAAGAACACACGATACACTGGTGGTTATTCTGAAGGGACCCGAACGATTAAAATCTTTTGGGAGGTAATTAGCGGATTTGAACCTAAAGAACGGTGTATGCTTCTGAAATTCGTGACCAGTTGTTCTCGAGCTCCATTACTTGGGTTTAAACACTTGCAACCAACGTTTACAATTCATAAGGCAATGCCACTTTACCAGTCATTGCTTTCACAACTTCCAACTTACAAGCGTCCAAGCACGTTGAGAGAAAAACTTCTATATGCAATCAGTTCTAACGCAGGTGTCCCTCCTCCGTTCTTTCGTTGCTTCCTTACTTTGGGATTGGAGTTATCAGACCCCTTGGATCTTGTAGGCTTATTTTAG

Protein Analysis

1133

Amino Acids

128.01

Weight (kDa)

8.47

Isoelectric Point (pI)

47.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HECT PF00632 816 - 1106 2e-78 HECT-domain (ubiquitin-transferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1920, 2159, 2883
AarI CACCTGC 3 cut(s) 1827, 2056, 3305
Acc36I ACCTGC 4 cut(s) 946, 1827, 2056, 3305
Acc65I GGTACC 1 cut(s) 1861
AccB1I GGYRCC 3 cut(s) 1242, 1861, 2013
AccB7I CCANNNNNTGG 3 cut(s) 1406, 2405, 3176
AccBSI CCGCTC 1 cut(s) 1652
AccI GTMKAC 1 cut(s) 1954
AccII CGCG 3 cut(s) 41, 120, 466
AciI CCGC 9 cut(s) 126, 466, 562, 629, 1652, 2062, 2075, 2171, 3108
AclI AACGTT 1 cut(s) 3200
AclWI GGATC 7 cut(s) 2, 1045, 1125, 1198, 2353, 2687, 3387
AcoI YGGCCR 1 cut(s) 1030
AcsI RAATTY 4 cut(s) 385, 1564, 2981, 3143
AcuI CTGAAG 3 cut(s) 128, 2796, 3087
AcyI GRCGYC 2 cut(s) 66, 1307
AfaI GTAC 6 cut(s) 753, 878, 930, 1159, 1863, 2861
AfiI CCNNNNNNNGG 8 cut(s) 1406, 2245, 2401, 2405, 2477, 2508, 3176, 3352
AflIII ACRYGT 3 cut(s) 299, 1776, 2797
AjnI CCWGG 8 cut(s) 254, 520, 1104, 1444, 1750, 2340, 2507, 2815
AjuI GAANNNNNNNTTGG 4 cut(s) 957, 989, 2769, 2801
Alw21I GWGCWC 3 cut(s) 195, 2811, 3169
Alw26I GTCTC 7 cut(s) 31, 57, 88, 519, 1166, 1292, 1767
AlwI GGATC 7 cut(s) 2, 1045, 1125, 1198, 2353, 2687, 3387
AlwNI CAGNNNCTG 2 cut(s) 919, 2405
Ama87I CYCGRG 3 cut(s) 74, 434, 3162
AoxI GGCC 9 cut(s) 278, 806, 838, 1030, 1223, 1748, 2263, 2392, 2407
ApeKI GCWGC 5 cut(s) 128, 131, 635, 2081, 2459
ApoI RAATTY 4 cut(s) 385, 1564, 2981, 3143
Asp700I GAANNNNTTC 2 cut(s) 1384, 3288
Asp718I GGTACC 1 cut(s) 1861
AspLEI GCGC 4 cut(s) 41, 47, 122, 162
AspS9I GGNCC 3 cut(s) 1754, 2264, 3072
AsuC2I CCSGG 1 cut(s) 2239
AsuHPI GGTGA 7 cut(s) 181, 683, 1000, 2037, 2264, 2750, 2940
AvaI CYCGRG 3 cut(s) 74, 434, 3162
AvaII GGWCC 2 cut(s) 1754, 3072
BaeI ACNNNNGTAYC 2 cut(s) 3040, 3073
BanI GGYRCC 3 cut(s) 1242, 1861, 2013
BanII GRGCYC 3 cut(s) 195, 2811, 3169
BauI CACGAG 1 cut(s) 1647
BbsI GAAGAC 1 cut(s) 537
Bbv12I GWGCWC 3 cut(s) 195, 2811, 3169
BbvCI CCTCAGC 1 cut(s) 2503
BbvI GCAGC 5 cut(s) 115, 118, 647, 2068, 2446
BceAI ACGGC 2 cut(s) 1017, 1901
BciT130I CCWGG 8 cut(s) 256, 522, 1106, 1446, 1752, 2342, 2509, 2817
BciVI GTATCC 1 cut(s) 2564
BclI TGATCA 1 cut(s) 1660
BcnI CCSGG 1 cut(s) 2239
BcoDI GTCTC 7 cut(s) 31, 57, 88, 519, 1166, 1292, 1767
BfaI CTAG 8 cut(s) 374, 1080, 1494, 1695, 2052, 2268, 2543, 2615
BfmI CTRYAG 2 cut(s) 163, 2923
BfoI RGCGCY 2 cut(s) 48, 163
BfuAI ACCTGC 4 cut(s) 946, 1827, 2056, 3305
BfuI GTATCC 1 cut(s) 2564
BglII AGATCT 1 cut(s) 1252
BisI GCNGC 6 cut(s) 129, 132, 563, 636, 2082, 2460
BlsI GCNGC 6 cut(s) 130, 133, 564, 637, 2083, 2461
BmcAI AGTACT 1 cut(s) 878
Bme1390I CCNGG 9 cut(s) 256, 522, 1106, 1446, 1752, 2239, 2342, 2509, 2817
Bme18I GGWCC 2 cut(s) 1754, 3072
BmeT110I CYCGRG 3 cut(s) 74, 434, 3162
BmgT120I GGNCC 3 cut(s) 1754, 2264, 3072
BmiI GGNNCC 5 cut(s) 1244, 1863, 2015, 3073, 3074
BmrFI CCNGG 9 cut(s) 256, 522, 1106, 1446, 1752, 2239, 2342, 2509, 2817
BmsI GCATC 5 cut(s) 28, 622, 709, 1015, 1970
BpiI GAAGAC 1 cut(s) 537
BplI GAGNNNNNCTC 4 cut(s) 68, 100, 863, 895
BpmI CTGGAG 1 cut(s) 1895
Bpu10I CCTNAGC 3 cut(s) 553, 1220, 2503
BpuEI CTTGAG 5 cut(s) 1106, 1272, 1743, 2795, 3052
BpuMI CCSGG 1 cut(s) 2239
Bsa29I ATCGAT 2 cut(s) 308, 692
BsaBI GATNNNNATC 1 cut(s) 3086
BsaHI GRCGYC 2 cut(s) 66, 1307
BsaI GGTCTC 2 cut(s) 31, 88
BsaXI ACNNNNNCTCC 4 cut(s) 2152, 2182, 2219, 2249
Bsc4I CCNNNNNNNGG 8 cut(s) 1406, 2245, 2401, 2405, 2477, 2508, 3176, 3352
Bse1I ACTGG 8 cut(s) 990, 1140, 1323, 1878, 2017, 3058, 3153, 3232
Bse3DI GCAATG 5 cut(s) 564, 948, 1412, 3225, 3236
Bse8I GATNNNNATC 1 cut(s) 3086
BseBI CCWGG 8 cut(s) 256, 522, 1106, 1446, 1752, 2342, 2509, 2817
BseCI ATCGAT 2 cut(s) 308, 692
BseGI GGATG 8 cut(s) 10, 637, 724, 859, 2360, 2364, 2833, 2862
BseJI GATNNNNATC 1 cut(s) 3086
BseLI CCNNNNNNNGG 8 cut(s) 1406, 2245, 2401, 2405, 2477, 2508, 3176, 3352
BseMI GCAATG 5 cut(s) 564, 948, 1412, 3225, 3236
BseMII CTCAG 9 cut(s) 101, 208, 817, 861, 926, 1247, 1287, 2463, 2517
BseNI ACTGG 8 cut(s) 990, 1140, 1323, 1878, 2017, 3058, 3153, 3232
BseRI GAGGAG 4 cut(s) 215, 334, 452, 3315
BseXI GCAGC 5 cut(s) 115, 118, 647, 2068, 2446
BseYI CCCAGC 1 cut(s) 1689
Bsh1236I CGCG 3 cut(s) 41, 120, 466
BshFI GGCC 9 cut(s) 280, 808, 840, 1032, 1225, 1750, 2265, 2394, 2409
BshNI GGYRCC 3 cut(s) 1242, 1861, 2013
BshVI ATCGAT 2 cut(s) 308, 692
BsiHKAI GWGCWC 3 cut(s) 195, 2811, 3169
BsiHKCI CYCGRG 3 cut(s) 74, 434, 3162
BsiSI CCGG 1 cut(s) 2239
BslFI GGGAC 3 cut(s) 1345, 3085, 3305
BslI CCNNNNNNNGG 8 cut(s) 1406, 2245, 2401, 2405, 2477, 2508, 3176, 3352
BsmAI GTCTC 7 cut(s) 31, 57, 88, 519, 1166, 1292, 1767
BsmBI CGTCTC 1 cut(s) 57
BsmFI GGGAC 3 cut(s) 1345, 3085, 3305
BsmI GAATGC 2 cut(s) 2462, 2918
BsnI GGCC 9 cut(s) 280, 808, 840, 1032, 1225, 1750, 2265, 2394, 2409
Bso31I GGTCTC 2 cut(s) 31, 88
BsoBI CYCGRG 3 cut(s) 74, 434, 3162
Bsp1286I GDGCHC 3 cut(s) 195, 2811, 3169
Bsp1407I TGTACA 2 cut(s) 751, 1157
Bsp19I CCATGG 1 cut(s) 1186
BspACI CCGC 9 cut(s) 126, 466, 562, 629, 1652, 2062, 2075, 2171, 3108
BspANI GGCC 9 cut(s) 280, 808, 840, 1032, 1225, 1750, 2265, 2394, 2409
BspCNI CTCAG 9 cut(s) 100, 207, 816, 862, 925, 1246, 1288, 2464, 2516
BspDI ATCGAT 2 cut(s) 308, 692
BspFNI CGCG 3 cut(s) 41, 120, 466
BspHI TCATGA 1 cut(s) 2092
BspLI GGNNCC 5 cut(s) 1244, 1863, 2015, 3073, 3074
BspMI ACCTGC 4 cut(s) 946, 1827, 2056, 3305
BspPI GGATC 7 cut(s) 2, 1045, 1125, 1198, 2353, 2687, 3387
BspQI GCTCTTC 1 cut(s) 398
BspT107I GGYRCC 3 cut(s) 1242, 1861, 2013
BspTNI GGTCTC 2 cut(s) 31, 88
BsrBI CCGCTC 1 cut(s) 1652
BsrDI GCAATG 5 cut(s) 564, 948, 1412, 3225, 3236
BsrGI TGTACA 2 cut(s) 751, 1157
BsrI ACTGG 8 cut(s) 990, 1140, 1323, 1878, 2017, 3058, 3153, 3232
BssNI GRCGYC 2 cut(s) 66, 1307
BssSI CACGAG 1 cut(s) 1647
BssT1I CCWWGG 7 cut(s) 48, 841, 902, 1186, 1400, 2572, 3375
Bst2BI CACGAG 1 cut(s) 1647
Bst2UI CCWGG 8 cut(s) 256, 522, 1106, 1446, 1752, 2342, 2509, 2817
Bst4CI ACNGT 7 cut(s) 620, 1162, 2652, 2754, 2770, 2836, 3129
Bst6I CTCTTC 7 cut(s) 143, 311, 347, 398, 593, 2572, 2772
BstACI GRCGYC 2 cut(s) 66, 1307
BstAUI TGTACA 2 cut(s) 751, 1157
BstDSI CCRYGG 2 cut(s) 1033, 1186
BstENI CCTNNNNNAGG 2 cut(s) 2399, 2506
BstF5I GGATG 8 cut(s) 10, 637, 724, 859, 2360, 2364, 2833, 2862
BstFNI CGCG 3 cut(s) 41, 120, 466
BstH2I RGCGCY 2 cut(s) 48, 163
BstHHI GCGC 4 cut(s) 41, 47, 122, 162
BstMAI GTCTC 7 cut(s) 31, 57, 88, 519, 1166, 1292, 1767
BstMWI GCNNNNNNNGC 7 cut(s) 119, 128, 635, 913, 1885, 2081, 2465
BstNI CCWGG 8 cut(s) 256, 522, 1106, 1446, 1752, 2342, 2509, 2817
BstNSI RCATGY 6 cut(s) 303, 490, 1432, 1780, 2177, 2801
BstSCI CCNGG 9 cut(s) 254, 520, 1104, 1444, 1750, 2237, 2340, 2507, 2815
BstSFI CTRYAG 2 cut(s) 163, 2923
BstUI CGCG 3 cut(s) 41, 120, 466
BstV1I GCAGC 5 cut(s) 115, 118, 647, 2068, 2446
BstV2I GAAGAC 1 cut(s) 537
BstX2I RGATCY 4 cut(s) 1117, 1190, 1252, 3379
BstYI RGATCY 4 cut(s) 1117, 1190, 1252, 3379
Bsu15I ATCGAT 2 cut(s) 308, 692
BsuI GTATCC 1 cut(s) 2564
BsuRI GGCC 9 cut(s) 280, 808, 840, 1032, 1225, 1750, 2265, 2394, 2409
BsuTUI ATCGAT 2 cut(s) 308, 692
BtgI CCRYGG 2 cut(s) 1033, 1186
BtsCI GGATG 8 cut(s) 10, 637, 724, 859, 2360, 2364, 2833, 2862
BtsI GCAGTG 4 cut(s) 246, 645, 1244, 1513
BveI ACCTGC 4 cut(s) 946, 1827, 2056, 3305
CaiI CAGNNNCTG 2 cut(s) 919, 2405
CciI TCATGA 1 cut(s) 2092
CfoI GCGC 4 cut(s) 41, 47, 122, 162
Cfr13I GGNCC 3 cut(s) 1754, 2264, 3072
ClaI ATCGAT 2 cut(s) 308, 692
CseI GACGC 7 cut(s) 74, 126, 472, 807, 905, 1315, 3254
Csp6I GTAC 6 cut(s) 752, 877, 929, 1158, 1862, 2860
CspCI CAANNNNNGTGG 4 cut(s) 322, 357, 2800, 2835
CviQI GTAC 6 cut(s) 752, 877, 929, 1158, 1862, 2860
DraI TTTAAA 1 cut(s) 3184
EaeI YGGCCR 1 cut(s) 1030
Eam1104I CTCTTC 7 cut(s) 143, 311, 347, 398, 593, 2572, 2772
EarI CTCTTC 7 cut(s) 143, 311, 347, 398, 593, 2572, 2772
Ecl136II GAGCTC 3 cut(s) 193, 2809, 3167
Eco130I CCWWGG 7 cut(s) 48, 841, 902, 1186, 1400, 2572, 3375
Eco147I AGGCCT 3 cut(s) 280, 1225, 1750
Eco24I GRGCYC 3 cut(s) 195, 2811, 3169
Eco31I GGTCTC 2 cut(s) 31, 88
Eco32I GATATC 2 cut(s) 1528, 2540
Eco47I GGWCC 2 cut(s) 1754, 3072
Eco53kI GAGCTC 3 cut(s) 193, 2809, 3167
Eco57I CTGAAG 3 cut(s) 128, 2796, 3087
Eco88I CYCGRG 3 cut(s) 74, 434, 3162
EcoICRI GAGCTC 3 cut(s) 193, 2809, 3167
EcoNI CCTNNNNNAGG 2 cut(s) 2399, 2506
EcoO109I RGGNCCY 1 cut(s) 3072
EcoRI GAATTC 1 cut(s) 385
EcoRII CCWGG 8 cut(s) 254, 520, 1104, 1444, 1750, 2340, 2507, 2815
EcoRV GATATC 2 cut(s) 1528, 2540
EcoT14I CCWWGG 7 cut(s) 48, 841, 902, 1186, 1400, 2572, 3375
EcoT22I ATGCAT 1 cut(s) 2920
EcoT38I GRGCYC 3 cut(s) 195, 2811, 3169
ErhI CCWWGG 7 cut(s) 48, 841, 902, 1186, 1400, 2572, 3375
Esp3I CGTCTC 1 cut(s) 57
FalI AAGNNNNNCTT 2 cut(s) 344, 376
FaqI GGGAC 3 cut(s) 1345, 3085, 3305
FbaI TGATCA 1 cut(s) 1660
FblI GTMKAC 1 cut(s) 1954
Fnu4HI GCNGC 6 cut(s) 129, 132, 563, 636, 2082, 2460
FokI GGATG 8 cut(s) 17, 644, 731, 866, 2347, 2351, 2840, 2849
FriOI GRGCYC 3 cut(s) 195, 2811, 3169
Fsp4HI GCNGC 6 cut(s) 129, 132, 563, 636, 2082, 2460
FspBI CTAG 8 cut(s) 374, 1080, 1494, 1695, 2052, 2268, 2543, 2615
GlaI GCGC 4 cut(s) 40, 46, 121, 161
GluI GCNGC 6 cut(s) 129, 132, 563, 636, 2082, 2460
GsaI CCCAGC 1 cut(s) 1693
GsuI CTGGAG 1 cut(s) 1895
HaeII RGCGCY 2 cut(s) 48, 163
HaeIII GGCC 9 cut(s) 280, 808, 840, 1032, 1225, 1750, 2265, 2394, 2409
HapII CCGG 1 cut(s) 2239
HgaI GACGC 7 cut(s) 74, 126, 472, 807, 905, 1315, 3254
HhaI GCGC 4 cut(s) 41, 47, 122, 162
Hin1I GRCGYC 2 cut(s) 66, 1307
Hin6I GCGC 4 cut(s) 39, 45, 120, 160
HinP1I GCGC 4 cut(s) 39, 45, 120, 160
HincII GTYRAC 3 cut(s) 343, 1096, 1705
HindII GTYRAC 3 cut(s) 343, 1096, 1705
HindIII AAGCTT 1 cut(s) 2885
HpaII CCGG 1 cut(s) 2239
HphI GGTGA 7 cut(s) 181, 683, 1000, 2037, 2264, 2750, 2940
Hpy166II GTNNAC 8 cut(s) 343, 752, 1096, 1440, 1705, 1955, 2273, 3204
Hpy8I GTNNAC 8 cut(s) 343, 752, 1096, 1440, 1705, 1955, 2273, 3204
Hpy99I CGWCG 1 cut(s) 2301
HpyAV CCTTC 7 cut(s) 73, 152, 637, 1634, 2405, 2597, 3062
HpyCH4III ACNGT 7 cut(s) 620, 1162, 2652, 2754, 2770, 2836, 3129
HpyCH4IV ACGT 4 cut(s) 548, 1334, 3200, 3275
HpyF10VI GCNNNNNNNGC 7 cut(s) 119, 128, 635, 913, 1885, 2081, 2465
HpySE526I ACGT 4 cut(s) 548, 1334, 3200, 3275
Hsp92I GRCGYC 2 cut(s) 66, 1307
HspAI GCGC 4 cut(s) 39, 45, 120, 160
KflI GGGWCCC 1 cut(s) 3072
KpnI GGTACC 1 cut(s) 1865
Ksp22I TGATCA 1 cut(s) 1660
LguI GCTCTTC 1 cut(s) 398
LmnI GCTCC 6 cut(s) 190, 727, 1469, 1876, 2473, 3172
Lsp1109I GCAGC 5 cut(s) 115, 118, 647, 2068, 2446
LweI GCATC 5 cut(s) 28, 622, 709, 1015, 1970
MaeI CTAG 8 cut(s) 374, 1080, 1494, 1695, 2052, 2268, 2543, 2615
MaeII ACGT 4 cut(s) 548, 1334, 3200, 3275
MaeIII GTNAC 8 cut(s) 169, 506, 732, 1280, 2770, 2830, 2836, 3148
MbiI CCGCTC 1 cut(s) 1652
MfeI CAATTG 3 cut(s) 423, 2070, 2871
MflI RGATCY 4 cut(s) 1117, 1190, 1252, 3379
MhlI GDGCHC 3 cut(s) 195, 2811, 3169
MlyI GAGTC 5 cut(s) 338, 389, 1091, 1976, 2789
MmeI TCCRAC 5 cut(s) 831, 988, 1317, 1455, 3278
Mph1103I ATGCAT 1 cut(s) 2920
MroXI GAANNNNTTC 2 cut(s) 1384, 3288
MslI CAYNNNNRTG 1 cut(s) 2733
MspA1I CMGCKG 2 cut(s) 128, 926
MspI CCGG 1 cut(s) 2239
MspR9I CCNGG 9 cut(s) 256, 522, 1106, 1446, 1752, 2239, 2342, 2509, 2817
MssI GTTTAAAC 1 cut(s) 3184
MunI CAATTG 3 cut(s) 423, 2070, 2871
Mva1269I GAATGC 2 cut(s) 2462, 2918
MvaI CCWGG 8 cut(s) 256, 522, 1106, 1446, 1752, 2342, 2509, 2817
MvnI CGCG 3 cut(s) 41, 120, 466
MwoI GCNNNNNNNGC 7 cut(s) 119, 128, 635, 913, 1885, 2081, 2465
NciI CCSGG 1 cut(s) 2239
NcoI CCATGG 1 cut(s) 1186
NlaIV GGNNCC 5 cut(s) 1244, 1863, 2015, 3073, 3074
NmuCI GTSAC 6 cut(s) 169, 506, 732, 1280, 2836, 3148
NsiI ATGCAT 1 cut(s) 2920
NspI RCATGY 6 cut(s) 303, 490, 1432, 1780, 2177, 2801
PaeR7I CTCGAG 3 cut(s) 74, 434, 3162
PagI TCATGA 1 cut(s) 2092
PaqCI CACCTGC 3 cut(s) 1827, 2056, 3305
PasI CCCWGGG 1 cut(s) 2341
PceI AGGCCT 3 cut(s) 280, 1225, 1750
PciI ACATGT 3 cut(s) 299, 1776, 2797
PciSI GCTCTTC 1 cut(s) 398
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PctI GAATGC 2 cut(s) 2462, 2918
PdmI GAANNNNTTC 2 cut(s) 1384, 3288
PfeI GAWTC 7 cut(s) 310, 447, 1396, 1715, 1828, 2126, 2710
PflFI GACNNNGTC 1 cut(s) 1100
PflMI CCANNNNNTGG 3 cut(s) 1406, 2405, 3176
PfoI TCCNGGA 3 cut(s) 1104, 1444, 2237
PkrI GCNGC 6 cut(s) 130, 133, 564, 637, 2083, 2461
PleI GAGTC 5 cut(s) 338, 389, 1091, 1975, 2788
PmeI GTTTAAAC 1 cut(s) 3184
PpsI GAGTC 5 cut(s) 338, 389, 1091, 1975, 2788
PpuMI RGGWCCY 1 cut(s) 3072
PscI ACATGT 3 cut(s) 299, 1776, 2797
PsiI TTATAA 3 cut(s) 1920, 2159, 2883
Psp124BI GAGCTC 3 cut(s) 195, 2811, 3169
Psp1406I AACGTT 1 cut(s) 3200
Psp5II RGGWCCY 1 cut(s) 3072
Psp6I CCWGG 8 cut(s) 254, 520, 1104, 1444, 1750, 2340, 2507, 2815
PspFI CCCAGC 1 cut(s) 1689
PspGI CCWGG 8 cut(s) 254, 520, 1104, 1444, 1750, 2340, 2507, 2815
PspN4I GGNNCC 5 cut(s) 1244, 1863, 2015, 3073, 3074
PspPI GGNCC 3 cut(s) 1754, 2264, 3072
PspPPI RGGWCCY 1 cut(s) 3072
PsrI GAACNNNNNNTAC 2 cut(s) 3032, 3064
PstNI CAGNNNCTG 2 cut(s) 919, 2405
PsuI RGATCY 4 cut(s) 1117, 1190, 1252, 3379
PsyI GACNNNGTC 1 cut(s) 1100
PvuII CAGCTG 1 cut(s) 926
RsaI GTAC 6 cut(s) 753, 878, 930, 1159, 1863, 2861
RsaNI GTAC 6 cut(s) 752, 877, 929, 1158, 1862, 2860
RseI CAYNNNNRTG 1 cut(s) 2733
SacI GAGCTC 3 cut(s) 195, 2811, 3169
SapI GCTCTTC 1 cut(s) 398
SatI GCNGC 6 cut(s) 129, 132, 563, 636, 2082, 2460
Sau96I GGNCC 3 cut(s) 1754, 2264, 3072
ScaI AGTACT 1 cut(s) 878
SchI GAGTC 5 cut(s) 338, 389, 1091, 1976, 2789
ScrFI CCNGG 9 cut(s) 256, 522, 1106, 1446, 1752, 2239, 2342, 2509, 2817
SduI GDGCHC 3 cut(s) 195, 2811, 3169
SfaNI GCATC 5 cut(s) 28, 622, 709, 1015, 1970
SfcI CTRYAG 2 cut(s) 163, 2923
Sfr274I CTCGAG 3 cut(s) 74, 434, 3162
SinI GGWCC 2 cut(s) 1754, 3072
SlaI CTCGAG 3 cut(s) 74, 434, 3162
SmiMI CAYNNNNRTG 1 cut(s) 2733
SmlI CTYRAG 8 cut(s) 74, 434, 1085, 1287, 1758, 2810, 3031, 3162
SmoI CTYRAG 8 cut(s) 74, 434, 1085, 1287, 1758, 2810, 3031, 3162
SseBI AGGCCT 3 cut(s) 280, 1225, 1750
SsiI CCGC 9 cut(s) 126, 466, 562, 629, 1652, 2062, 2075, 2171, 3108
SspI AATATT 2 cut(s) 1015, 1325
SspMI CTAG 8 cut(s) 374, 1080, 1494, 1695, 2052, 2268, 2543, 2615
SstI GAGCTC 3 cut(s) 195, 2811, 3169
StuI AGGCCT 3 cut(s) 280, 1225, 1750
StyD4I CCNGG 9 cut(s) 254, 520, 1104, 1444, 1750, 2237, 2340, 2507, 2815
StyI CCWWGG 7 cut(s) 48, 841, 902, 1186, 1400, 2572, 3375
TaaI ACNGT 7 cut(s) 620, 1162, 2652, 2754, 2770, 2836, 3129
TaiI ACGT 4 cut(s) 551, 1337, 3203, 3278
TaqI TCGA 9 cut(s) 75, 308, 398, 435, 692, 2412, 2659, 2673, 3163
TaqII GACCGA 1 cut(s) 2459
TatI WGTACW 5 cut(s) 751, 876, 928, 1157, 2859
TauI GCSGC 1 cut(s) 565
TfiI GAWTC 7 cut(s) 310, 447, 1396, 1715, 1828, 2126, 2710
TseFI GTSAC 6 cut(s) 169, 506, 732, 1280, 2836, 3148
TseI GCWGC 5 cut(s) 128, 131, 635, 2081, 2459
Tsp45I GTSAC 6 cut(s) 169, 506, 732, 1280, 2836, 3148
TspGWI ACGGA 2 cut(s) 751, 3318
Tth111I GACNNNGTC 1 cut(s) 1100
Van91I CCANNNNNTGG 3 cut(s) 1406, 2405, 3176
VpaK11BI GGWCC 2 cut(s) 1754, 3072
XagI CCTNNNNNAGG 2 cut(s) 2399, 2506
XapI RAATTY 4 cut(s) 385, 1564, 2981, 3143
XbaI TCTAGA 1 cut(s) 2542
XceI RCATGY 6 cut(s) 303, 490, 1432, 1780, 2177, 2801
XhoI CTCGAG 3 cut(s) 74, 434, 3162
XmiI GTMKAC 1 cut(s) 1954
XmnI GAANNNNTTC 2 cut(s) 1384, 3288
XspI CTAG 8 cut(s) 374, 1080, 1494, 1695, 2052, 2268, 2543, 2615
ZrmI AGTACT 1 cut(s) 878
Zsp2I ATGCAT 1 cut(s) 2920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.