RLG00000024156

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0010433
Physical Location & Seq
Forward (+)
32561808 .. 32569264
7457 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024156

Sequence Viewer

Length: 3009 bp
ATGTTGTTGCAGAGAGTGTGGAGGCGCTATAGGGTGACTAAGGTGGTGGCTTTGGAGCTCAGAGAGGAGTGGGAGAAGTGTGTGAAGCAGAAGCAAGCTGGTTTAGTCATCACAGCAACCTGGATTTCTAGTCATGTAGTGAGGCCTTTACTTTTCTTCATTACATGTTTATCGATTCGGAAGAGGAGAATTGAAGCCACAGAAGTTGACTCTATGAAGAGTTGCTTTCAAATGCTACTAGACAGCATGAATTCCACTGACTCGAAGAAGAGCTATTGCTCTCAGGCAATTGGAACTCTCGAGGAGAGAAGAATCTGGGGTTATCAGACGCGGAAGCTGATTTCTCTTTGCATGTTTGTTCTTTCGGAATGTGACAAGTCCTGCCCAGGAAGTCAAGACATTGTTGCTCTTGCGTCCTTGGCAATGAGTCTTGTTAGTATTGATGAACATGACTGTCAGATTGCGGATGCAGCAGTGAAGGATTTAGTTCGGTTTATGGGGAGTGGTGAAAGTGGTCTTTACTCATCGATTAGAATATACATTAACACATTGGATGCTCCTTGCTCTTCACGGACAAAAAGTAGTGTACAAACAGATGACAGATTTTTGATTACTGCAAGCACAATAACTTTGGCTCTCAGGCCATTTCATGCGTCAAAGTCCGATCTAAATGGCCTTGGCTTGTTGGATGTCCATAATGTTGCTGAGAAGTACTCTGTGTTTCTGCTTACAATTCCTTGGCTTAATCAGCGTCTGCCAGCTGTACTTATACCTGCAATGAGGCACAAATCCATTCTCCAACCTTGTTTTCAAACATTACTGATTTTGAAAGAGAAAATATTAAAGGAGATGCTGGCCGTGGATCAGTCAAAGTTTCATGTTTCCCCCAAGGTTATTCCACCAGTTGGTTGGGCTCTTGCAAACATTATATGCCTAGCTACAGGGGGAGAGAGTGATTCTGTTGATCCTGGAGGTTTCTATCAAGAGTTGGATTGTGCATCTTATGTACGTGCTGTTAATAGTCTTGCAGAAAACTTATTGTCTAGGCTTGAGAATGTTGACTCTGTCCAGGAGAATCAGGATCTCCAAAGTGAAGTTGAAACCAGTGAAAAGCCAAGCTGTACAGTTTCATGTGAGACTGAAATGAACCATGGATCTAGTAAGTTGTCGTTCTTGGATATGCTTAGGCCTGTTTCTCAGCAGTGGCACCTTACAGATCTTTCGGCAATAGTGAATAAACTAGGTCACACTCAAGGGTCTGAGACTATGACGCCAAAAAGATTGGAATATTCAGGGACGTTGGAATTGCTGGATATTGTGCATTTTTATTCTTTCATGCTAAGAATGCTTTCATTCTTGAATCCAAGGGTTGGCTCATTGCCTGTCCTTAACATGCTATCGTTTACTCCTGGATTTCTTGTAAGTCTATGGGGAGCGTTGGAAACCTATCTTTTTCCTAGGAATGTTTGCAGTGGTCATAAACCCTATGGGAATTTTGGAAAAAGGAAAAAGCATGGCAATAATGATGGAGGTAATAAGTGGGTCCGTGTCCTTCATAAAATTACGGGTAAATCACAAGCGGAGATTGATCATACAGATTTGCGTGGTAATCAACCCAAGCCTAGACTTGTTGACAAAGAGGATTCTTCTGATGTTTGGGATATAGAACCTGTGAGGCCTGGTCTTCAAGGTATTTCAAGAGACATGTCATGTATGCTTCATCTATTCTGTGCAAGCTATTCACCCCTGCTTTTGATTCTTGATGACATAGAGTTTTATGAAAAACAGTTACCATTTACACTGGAGCAACAACGGCAAATTGCTTCTGTTCTCAATACATTGGTTTATAATGGGTTTCCTCAAAGTATTGGACAGGAGGGTAGACCCCTTATGGAGTCTGCTTTCTGCCCCCCTGTTTTGTGGCTTTCACCTGCTAGAAAGAACCGCCCACCAATTGCGGTAGCTGCCAGAACTCATGAAGTTTTGTCAGCTAATCAAAGATCAGATGATTCTCTGGCTGTGCAGGGTATAGGCTCTGTTATAACTACAACTCCGCATGTATTTCCATTTGAAGAAAGAGCGGAAATGTTTAGAGAGTTTATCAAGATGGACAAAGCCTCCCGGAAAATGGCTGGTGAGGTTTCTAGCCCTAGTTCACGATCAGTTGAGATAGTAGTTCGTCGTGGTCATATTGTTGAAGATGGATTTCAGCAGTTAAACTCCCTGGGATCAAGATTAAAGTCACCCATCCATGTTTCATTTGTCAGTGAATGTGGCCTTCCAGAGGCTGGCCTCGACTATGGTGGATTATCTAATGAGTTTTTGACCGATATATCAAAAGCTGCATTCGCTCCTGAGTATGGGCTATTCTCCCAGACCTCAACCTCAGCCAGACTTCTAATTCCTAATCCATCTGCAAGATATCTAGAGAATGGTATCCAGATGATTGAGTTCCTTGGAAGAGTTGTCGGGAAAGCTCTCTATGAAGGAATACTGCTAGATCACTCATTTTCTCATGTTTTTGTACAAAAACTGTTGGGTCGATATAGCTTCCTCAATGAACTATCAACACTTGATCCAGAGATTTACAGGAATCTCATGTATGTTAAGCATTATGATGGTGATGTTGAGGAACTGTGGCTGGACTTTACGCTCAAGCCTGGTGGAAAGGATGTCACTGTGACGAGCAAGAACAAAATGCAGTACATCCATGCAATTGCTGATTATAAGCTTAACCGACAGATGTTCCTTTTTTCAAATGCATTCTATAGAGGGTTAATTGATCTTATATCACCATCTTGGCTAAAATTATTTAATGCAGGGGAATTTAATCAGTTGCTTTCAGGTGGAATCCATGACATTGATGTTGATGACTTGAGAAAGAACACACGATACACTGGTGGTTACTCTGAAGGGACCCGAACGATTAAAATCTTTTGGGAGGTAATTAGTGGATTTGAACCTAAAGAACGGTGTAGGCTTCTGAAATTCGTGACCCGTTGTTCTTGA

Protein Analysis

1003

Amino Acids

112.78

Weight (kDa)

7.29

Isoelectric Point (pI)

45.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HECT PF00632 777 - 1001 1.1e-57 HECT-domain (ubiquitin-transferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1848, 2042, 2727
AarI CACCTGC 1 cut(s) 1939
Acc36I ACCTGC 2 cut(s) 781, 1939
AccB1I GGYRCC 1 cut(s) 1206
AccB7I CCANNNNNTGG 3 cut(s) 905, 1370, 2288
AccBSI CCGCTC 1 cut(s) 2081
AccI GTMKAC 1 cut(s) 1882
AccII CGCG 1 cut(s) 331
AciI CCGC 7 cut(s) 331, 464, 1580, 1945, 1958, 2054, 2081
AclWI GGATC 6 cut(s) 870, 959, 1089, 1162, 2236, 2570
AcoI YGGCCR 1 cut(s) 855
AcsI RAATTY 4 cut(s) 250, 1492, 2825, 2987
AcuI CTGAAG 1 cut(s) 2931
AcyI GRCGYC 1 cut(s) 1271
AfaI GTAC 7 cut(s) 588, 713, 765, 1008, 1123, 2526, 2705
AfiI CCNNNNNNNGG 6 cut(s) 905, 1370, 2128, 2284, 2288, 2360
AflIII ACRYGT 2 cut(s) 164, 1704
AjnI CCWGG 8 cut(s) 119, 385, 967, 1068, 1408, 1678, 2223, 2659
AjuI GAANNNNNNNTTGG 2 cut(s) 792, 824
Alw21I GWGCWC 1 cut(s) 60
Alw26I GTCTC 3 cut(s) 1130, 1256, 1695
AlwI GGATC 6 cut(s) 870, 959, 1089, 1162, 2236, 2570
AlwNI CAGNNNCTG 2 cut(s) 754, 2288
Ama87I CYCGRG 1 cut(s) 299
AoxI GGCC 8 cut(s) 143, 641, 673, 855, 1187, 1676, 2275, 2290
ApeKI GCWGC 3 cut(s) 470, 1964, 2342
ApoI RAATTY 4 cut(s) 250, 1492, 2825, 2987
Asp700I GAANNNNTTC 1 cut(s) 1348
AspA2I CCTAGG 1 cut(s) 1457
AspLEI GCGC 1 cut(s) 27
AspS9I GGNCC 2 cut(s) 1543, 2916
AsuC2I CCSGG 1 cut(s) 2122
AsuHPI GGTGA 8 cut(s) 46, 518, 1734, 1920, 2147, 2235, 2633, 2784
AvaI CYCGRG 1 cut(s) 299
AvaII GGWCC 2 cut(s) 1543, 2916
AvrII CCTAGG 1 cut(s) 1457
BaeI ACNNNNGTAYC 2 cut(s) 2884, 2917
BanI GGYRCC 1 cut(s) 1206
BanII GRGCYC 2 cut(s) 60, 916
BbsI GAAGAC 1 cut(s) 1676
Bbv12I GWGCWC 1 cut(s) 60
BbvCI CCTCAGC 1 cut(s) 2386
BbvI GCAGC 3 cut(s) 482, 1951, 2329
BccI CCATC 7 cut(s) 1520, 2101, 2195, 2255, 2419, 2612, 2803
BceAI ACGGC 2 cut(s) 842, 1829
BciT130I CCWGG 8 cut(s) 121, 387, 969, 1070, 1410, 1680, 2225, 2661
BciVI GTATCC 1 cut(s) 2447
BclI TGATCA 1 cut(s) 1588
BcnI CCSGG 1 cut(s) 2122
BcoDI GTCTC 3 cut(s) 1130, 1256, 1695
BfmI CTRYAG 3 cut(s) 28, 939, 2767
BfoI RGCGCY 1 cut(s) 28
BfuAI ACCTGC 2 cut(s) 781, 1939
BfuI GTATCC 1 cut(s) 2447
BglII AGATCT 1 cut(s) 1216
BisI GCNGC 3 cut(s) 471, 1965, 2343
BlnI CCTAGG 1 cut(s) 1457
BlsI GCNGC 3 cut(s) 472, 1966, 2344
BmcAI AGTACT 1 cut(s) 713
Bme1390I CCNGG 9 cut(s) 121, 387, 969, 1070, 1410, 1680, 2122, 2225, 2661
Bme18I GGWCC 2 cut(s) 1543, 2916
BmeT110I CYCGRG 1 cut(s) 299
BmgT120I GGNCC 2 cut(s) 1543, 2916
BmiI GGNNCC 4 cut(s) 1208, 1544, 2917, 2918
BmrFI CCNGG 9 cut(s) 121, 387, 969, 1070, 1410, 1680, 2122, 2225, 2661
BmsI GCATC 4 cut(s) 457, 544, 840, 1007
BpiI GAAGAC 1 cut(s) 1676
BplI GAGNNNNNCTC 2 cut(s) 698, 730
BpmI CTGGAG 2 cut(s) 990, 1823
Bpu10I CCTNAGC 2 cut(s) 1184, 2386
BpuEI CTTGAG 4 cut(s) 1070, 1236, 2639, 2896
BpuMI CCSGG 1 cut(s) 2122
Bsa29I ATCGAT 2 cut(s) 173, 527
BsaAI YACGTR 1 cut(s) 1010
BsaBI GATNNNNATC 1 cut(s) 2930
BsaHI GRCGYC 1 cut(s) 1271
BsaXI ACNNNNNCTCC 4 cut(s) 2035, 2065, 2102, 2132
Bsc4I CCNNNNNNNGG 6 cut(s) 905, 1370, 2128, 2284, 2288, 2360
Bse1I ACTGG 4 cut(s) 902, 1104, 1806, 2902
Bse3DI GCAATG 3 cut(s) 429, 783, 1376
Bse8I GATNNNNATC 1 cut(s) 2930
BseBI CCWGG 8 cut(s) 121, 387, 969, 1070, 1410, 1680, 2225, 2661
BseCI ATCGAT 2 cut(s) 173, 527
BseGI GGATG 6 cut(s) 472, 559, 694, 2247, 2677, 2706
BseJI GATNNNNATC 1 cut(s) 2930
BseLI CCNNNNNNNGG 6 cut(s) 905, 1370, 2128, 2284, 2288, 2360
BseMI GCAATG 3 cut(s) 429, 783, 1376
BseMII CTCAG 8 cut(s) 73, 296, 652, 696, 1211, 1251, 2346, 2400
BseNI ACTGG 4 cut(s) 902, 1104, 1806, 2902
BseRI GAGGAG 3 cut(s) 80, 199, 317
BseXI GCAGC 3 cut(s) 482, 1951, 2329
BsgI GTGCAG 1 cut(s) 2042
Bsh1236I CGCG 1 cut(s) 331
BshFI GGCC 8 cut(s) 145, 643, 675, 857, 1189, 1678, 2277, 2292
BshNI GGYRCC 1 cut(s) 1206
BshVI ATCGAT 2 cut(s) 173, 527
BsiHKAI GWGCWC 1 cut(s) 60
BsiHKCI CYCGRG 1 cut(s) 299
BsiSI CCGG 1 cut(s) 2122
BslFI GGGAC 2 cut(s) 1309, 2929
BslI CCNNNNNNNGG 6 cut(s) 905, 1370, 2128, 2284, 2288, 2360
BsmAI GTCTC 3 cut(s) 1130, 1256, 1695
BsmFI GGGAC 2 cut(s) 1309, 2929
BsmI GAATGC 3 cut(s) 1350, 2345, 2762
BsnI GGCC 8 cut(s) 145, 643, 675, 857, 1189, 1678, 2277, 2292
BsoBI CYCGRG 1 cut(s) 299
Bsp1286I GDGCHC 2 cut(s) 60, 916
Bsp1407I TGTACA 3 cut(s) 586, 1121, 2524
Bsp19I CCATGG 1 cut(s) 1150
BspACI CCGC 7 cut(s) 331, 464, 1580, 1945, 1958, 2054, 2081
BspANI GGCC 8 cut(s) 145, 643, 675, 857, 1189, 1678, 2277, 2292
BspCNI CTCAG 8 cut(s) 72, 295, 651, 697, 1210, 1252, 2347, 2399
BspDI ATCGAT 2 cut(s) 173, 527
BspFNI CGCG 1 cut(s) 331
BspHI TCATGA 1 cut(s) 1975
BspLI GGNNCC 4 cut(s) 1208, 1544, 2917, 2918
BspMI ACCTGC 2 cut(s) 781, 1939
BspPI GGATC 6 cut(s) 870, 959, 1089, 1162, 2236, 2570
BspQI GCTCTTC 2 cut(s) 263, 571
BspT107I GGYRCC 1 cut(s) 1206
BsrBI CCGCTC 1 cut(s) 2081
BsrDI GCAATG 3 cut(s) 429, 783, 1376
BsrGI TGTACA 3 cut(s) 586, 1121, 2524
BsrI ACTGG 4 cut(s) 902, 1104, 1806, 2902
BssNI GRCGYC 1 cut(s) 1271
BssT1I CCWWGG 8 cut(s) 417, 676, 737, 888, 1150, 1364, 1457, 2455
Bst2UI CCWGG 8 cut(s) 121, 387, 969, 1070, 1410, 1680, 2225, 2661
Bst4CI ACNGT 7 cut(s) 455, 1126, 1788, 2535, 2637, 2680, 2973
Bst6I CTCTTC 5 cut(s) 176, 212, 263, 571, 2455
BstACI GRCGYC 1 cut(s) 1271
BstAUI TGTACA 3 cut(s) 586, 1121, 2524
BstBAI YACGTR 1 cut(s) 1010
BstC8I GCNNGC 6 cut(s) 96, 619, 759, 855, 1735, 2290
BstDSI CCRYGG 2 cut(s) 858, 1150
BstENI CCTNNNNNAGG 1 cut(s) 2282
BstF5I GGATG 6 cut(s) 472, 559, 694, 2247, 2677, 2706
BstFNI CGCG 1 cut(s) 331
BstH2I RGCGCY 1 cut(s) 28
BstHHI GCGC 1 cut(s) 27
BstMAI GTCTC 3 cut(s) 1130, 1256, 1695
BstMWI GCNNNNNNNGC 7 cut(s) 419, 470, 748, 1345, 1813, 1964, 2348
BstNI CCWGG 8 cut(s) 121, 387, 969, 1070, 1410, 1680, 2225, 2661
BstNSI RCATGY 5 cut(s) 168, 355, 1396, 1708, 2060
BstSCI CCNGG 9 cut(s) 119, 385, 967, 1068, 1408, 1678, 2120, 2223, 2659
BstSFI CTRYAG 3 cut(s) 28, 939, 2767
BstUI CGCG 1 cut(s) 331
BstV1I GCAGC 3 cut(s) 482, 1951, 2329
BstV2I GAAGAC 1 cut(s) 1676
BstX2I RGATCY 3 cut(s) 1081, 1154, 1216
BstXI CCANNNNNNTGG 1 cut(s) 909
BstYI RGATCY 3 cut(s) 1081, 1154, 1216
Bsu15I ATCGAT 2 cut(s) 173, 527
BsuI GTATCC 1 cut(s) 2447
BsuRI GGCC 8 cut(s) 145, 643, 675, 857, 1189, 1678, 2277, 2292
BsuTUI ATCGAT 2 cut(s) 173, 527
BtgI CCRYGG 2 cut(s) 858, 1150
BtsCI GGATG 6 cut(s) 472, 559, 694, 2247, 2677, 2706
BtsI GCAGTG 3 cut(s) 480, 1208, 1477
BtsIMutI CAGTG 9 cut(s) 255, 480, 1111, 1208, 1477, 1799, 2272, 2676, 2895
BveI ACCTGC 2 cut(s) 781, 1939
Cac8I GCNNGC 6 cut(s) 96, 619, 759, 855, 1735, 2290
CaiI CAGNNNCTG 2 cut(s) 754, 2288
CciI TCATGA 1 cut(s) 1975
CfoI GCGC 1 cut(s) 27
Cfr13I GGNCC 2 cut(s) 1543, 2916
ClaI ATCGAT 2 cut(s) 173, 527
CseI GACGC 5 cut(s) 337, 402, 642, 740, 1279
Csp6I GTAC 7 cut(s) 587, 712, 764, 1007, 1122, 2525, 2704
CspCI CAANNNNNGTGG 4 cut(s) 187, 222, 2644, 2679
CviQI GTAC 7 cut(s) 587, 712, 764, 1007, 1122, 2525, 2704
EaeI YGGCCR 1 cut(s) 855
Eam1104I CTCTTC 5 cut(s) 176, 212, 263, 571, 2455
EarI CTCTTC 5 cut(s) 176, 212, 263, 571, 2455
Ecl136II GAGCTC 1 cut(s) 58
Eco130I CCWWGG 8 cut(s) 417, 676, 737, 888, 1150, 1364, 1457, 2455
Eco147I AGGCCT 3 cut(s) 145, 1189, 1678
Eco24I GRGCYC 2 cut(s) 60, 916
Eco32I GATATC 1 cut(s) 2423
Eco47I GGWCC 2 cut(s) 1543, 2916
Eco53kI GAGCTC 1 cut(s) 58
Eco57I CTGAAG 1 cut(s) 2931
Eco88I CYCGRG 1 cut(s) 299
EcoICRI GAGCTC 1 cut(s) 58
EcoNI CCTNNNNNAGG 1 cut(s) 2282
EcoO109I RGGNCCY 1 cut(s) 2916
EcoRI GAATTC 1 cut(s) 250
EcoRII CCWGG 8 cut(s) 119, 385, 967, 1068, 1408, 1678, 2223, 2659
EcoRV GATATC 1 cut(s) 2423
EcoT14I CCWWGG 8 cut(s) 417, 676, 737, 888, 1150, 1364, 1457, 2455
EcoT22I ATGCAT 1 cut(s) 2764
EcoT38I GRGCYC 2 cut(s) 60, 916
ErhI CCWWGG 8 cut(s) 417, 676, 737, 888, 1150, 1364, 1457, 2455
FalI AAGNNNNNCTT 2 cut(s) 209, 241
FaqI GGGAC 2 cut(s) 1309, 2929
FbaI TGATCA 1 cut(s) 1588
FblI GTMKAC 1 cut(s) 1882
Fnu4HI GCNGC 3 cut(s) 471, 1965, 2343
FokI GGATG 6 cut(s) 479, 566, 701, 2234, 2684, 2693
FriOI GRGCYC 2 cut(s) 60, 916
Fsp4HI GCNGC 3 cut(s) 471, 1965, 2343
GlaI GCGC 1 cut(s) 26
GluI GCNGC 3 cut(s) 471, 1965, 2343
GsuI CTGGAG 2 cut(s) 990, 1823
HaeII RGCGCY 1 cut(s) 28
HaeIII GGCC 8 cut(s) 145, 643, 675, 857, 1189, 1678, 2277, 2292
HapII CCGG 1 cut(s) 2122
HgaI GACGC 5 cut(s) 337, 402, 642, 740, 1279
HhaI GCGC 1 cut(s) 27
Hin1I GRCGYC 1 cut(s) 1271
Hin6I GCGC 1 cut(s) 25
HinP1I GCGC 1 cut(s) 25
HincII GTYRAC 3 cut(s) 208, 1060, 1633
HindII GTYRAC 3 cut(s) 208, 1060, 1633
HindIII AAGCTT 1 cut(s) 2729
HpaII CCGG 1 cut(s) 2122
HphI GGTGA 8 cut(s) 46, 518, 1734, 1920, 2147, 2235, 2633, 2784
Hpy166II GTNNAC 7 cut(s) 208, 587, 1060, 1404, 1633, 1883, 2156
Hpy8I GTNNAC 7 cut(s) 208, 587, 1060, 1404, 1633, 1883, 2156
Hpy99I CGWCG 1 cut(s) 2184
HpyAV CCTTC 5 cut(s) 472, 1562, 2288, 2480, 2906
HpyCH4III ACNGT 7 cut(s) 455, 1126, 1788, 2535, 2637, 2680, 2973
HpyCH4IV ACGT 2 cut(s) 1009, 1298
HpyF10VI GCNNNNNNNGC 7 cut(s) 419, 470, 748, 1345, 1813, 1964, 2348
HpySE526I ACGT 2 cut(s) 1009, 1298
Hsp92I GRCGYC 1 cut(s) 1271
HspAI GCGC 1 cut(s) 25
KflI GGGWCCC 1 cut(s) 2916
Ksp22I TGATCA 1 cut(s) 1588
LguI GCTCTTC 2 cut(s) 263, 571
LmnI GCTCC 5 cut(s) 55, 562, 1433, 1804, 2356
Lsp1109I GCAGC 3 cut(s) 482, 1951, 2329
LweI GCATC 4 cut(s) 457, 544, 840, 1007
MaeII ACGT 2 cut(s) 1009, 1298
MaeIII GTNAC 9 cut(s) 34, 371, 1244, 1788, 2241, 2674, 2680, 2903, 2992
MbiI CCGCTC 1 cut(s) 2081
MfeI CAATTG 3 cut(s) 288, 1953, 2715
MflI RGATCY 3 cut(s) 1081, 1154, 1216
MhlI GDGCHC 2 cut(s) 60, 916
MlyI GAGTC 5 cut(s) 203, 254, 436, 1055, 1904
MmeI TCCRAC 5 cut(s) 666, 823, 969, 1281, 1419
Mph1103I ATGCAT 1 cut(s) 2764
MroXI GAANNNNTTC 1 cut(s) 1348
MslI CAYNNNNRTG 1 cut(s) 2616
MspA1I CMGCKG 1 cut(s) 761
MspI CCGG 1 cut(s) 2122
MspR9I CCNGG 9 cut(s) 121, 387, 969, 1070, 1410, 1680, 2122, 2225, 2661
MunI CAATTG 3 cut(s) 288, 1953, 2715
Mva1269I GAATGC 3 cut(s) 1350, 2345, 2762
MvaI CCWGG 8 cut(s) 121, 387, 969, 1070, 1410, 1680, 2225, 2661
MvnI CGCG 1 cut(s) 331
MwoI GCNNNNNNNGC 7 cut(s) 419, 470, 748, 1345, 1813, 1964, 2348
NciI CCSGG 1 cut(s) 2122
NcoI CCATGG 1 cut(s) 1150
NlaIV GGNNCC 4 cut(s) 1208, 1544, 2917, 2918
NmuCI GTSAC 7 cut(s) 34, 371, 1244, 2241, 2674, 2680, 2992
NsiI ATGCAT 1 cut(s) 2764
NspI RCATGY 5 cut(s) 168, 355, 1396, 1708, 2060
PaeR7I CTCGAG 1 cut(s) 299
PagI TCATGA 1 cut(s) 1975
PaqCI CACCTGC 1 cut(s) 1939
PasI CCCWGGG 1 cut(s) 2224
PceI AGGCCT 3 cut(s) 145, 1189, 1678
PciI ACATGT 2 cut(s) 164, 1704
PciSI GCTCTTC 2 cut(s) 263, 571
PctI GAATGC 3 cut(s) 1350, 2345, 2762
PdmI GAANNNNTTC 1 cut(s) 1348
PflFI GACNNNGTC 1 cut(s) 1064
PflMI CCANNNNNTGG 3 cut(s) 905, 1370, 2288
PfoI TCCNGGA 4 cut(s) 967, 1068, 1408, 2120
PkrI GCNGC 3 cut(s) 472, 1966, 2344
PleI GAGTC 5 cut(s) 203, 254, 435, 1055, 1903
PpsI GAGTC 5 cut(s) 203, 254, 435, 1055, 1903
Ppu21I YACGTR 1 cut(s) 1010
PpuMI RGGWCCY 1 cut(s) 2916
PscI ACATGT 2 cut(s) 164, 1704
PsiI TTATAA 3 cut(s) 1848, 2042, 2727
Psp124BI GAGCTC 1 cut(s) 60
Psp5II RGGWCCY 1 cut(s) 2916
Psp6I CCWGG 8 cut(s) 119, 385, 967, 1068, 1408, 1678, 2223, 2659
PspGI CCWGG 8 cut(s) 119, 385, 967, 1068, 1408, 1678, 2223, 2659
PspN4I GGNNCC 4 cut(s) 1208, 1544, 2917, 2918
PspPI GGNCC 2 cut(s) 1543, 2916
PspPPI RGGWCCY 1 cut(s) 2916
PsrI GAACNNNNNNTAC 2 cut(s) 2876, 2908
PstNI CAGNNNCTG 2 cut(s) 754, 2288
PsuI RGATCY 3 cut(s) 1081, 1154, 1216
PsyI GACNNNGTC 1 cut(s) 1064
PvuII CAGCTG 1 cut(s) 761
RsaI GTAC 7 cut(s) 588, 713, 765, 1008, 1123, 2526, 2705
RsaNI GTAC 7 cut(s) 587, 712, 764, 1007, 1122, 2525, 2704
RseI CAYNNNNRTG 1 cut(s) 2616
SacI GAGCTC 1 cut(s) 60
SapI GCTCTTC 2 cut(s) 263, 571
SatI GCNGC 3 cut(s) 471, 1965, 2343
Sau96I GGNCC 2 cut(s) 1543, 2916
ScaI AGTACT 1 cut(s) 713
SchI GAGTC 5 cut(s) 203, 254, 436, 1055, 1904
ScrFI CCNGG 9 cut(s) 121, 387, 969, 1070, 1410, 1680, 2122, 2225, 2661
SduI GDGCHC 2 cut(s) 60, 916
SfaNI GCATC 4 cut(s) 457, 544, 840, 1007
SfcI CTRYAG 3 cut(s) 28, 939, 2767
Sfr274I CTCGAG 1 cut(s) 299
SinI GGWCC 2 cut(s) 1543, 2916
SlaI CTCGAG 1 cut(s) 299
SmiMI CAYNNNNRTG 1 cut(s) 2616
SmlI CTYRAG 5 cut(s) 299, 1049, 1251, 2654, 2875
SmoI CTYRAG 5 cut(s) 299, 1049, 1251, 2654, 2875
SseBI AGGCCT 3 cut(s) 145, 1189, 1678
SsiI CCGC 7 cut(s) 331, 464, 1580, 1945, 1958, 2054, 2081
SspI AATATT 2 cut(s) 840, 1289
SstI GAGCTC 1 cut(s) 60
StuI AGGCCT 3 cut(s) 145, 1189, 1678
StyD4I CCNGG 9 cut(s) 119, 385, 967, 1068, 1408, 1678, 2120, 2223, 2659
StyI CCWWGG 8 cut(s) 417, 676, 737, 888, 1150, 1364, 1457, 2455
TaaI ACNGT 7 cut(s) 455, 1126, 1788, 2535, 2637, 2680, 2973
TaiI ACGT 2 cut(s) 1012, 1301
TaqI TCGA 6 cut(s) 173, 263, 300, 527, 2295, 2542
TaqII GACCGA 1 cut(s) 2342
TatI WGTACW 6 cut(s) 586, 711, 763, 1121, 2524, 2703
TscAI CASTG 9 cut(s) 262, 480, 1111, 1208, 1477, 1806, 2272, 2683, 2902
TseFI GTSAC 7 cut(s) 34, 371, 1244, 2241, 2674, 2680, 2992
TseI GCWGC 3 cut(s) 470, 1964, 2342
Tsp45I GTSAC 7 cut(s) 34, 371, 1244, 2241, 2674, 2680, 2992
TspGWI ACGGA 2 cut(s) 586, 1535
TspRI CASTG 9 cut(s) 262, 480, 1111, 1208, 1477, 1806, 2272, 2683, 2902
Tth111I GACNNNGTC 1 cut(s) 1064
Van91I CCANNNNNTGG 3 cut(s) 905, 1370, 2288
VpaK11BI GGWCC 2 cut(s) 1543, 2916
XagI CCTNNNNNAGG 1 cut(s) 2282
XapI RAATTY 4 cut(s) 250, 1492, 2825, 2987
XbaI TCTAGA 1 cut(s) 2425
XceI RCATGY 5 cut(s) 168, 355, 1396, 1708, 2060
XcmI CCANNNNNNNNNTGG 1 cut(s) 906
XhoI CTCGAG 1 cut(s) 299
XmaJI CCTAGG 1 cut(s) 1457
XmiI GTMKAC 1 cut(s) 1882
XmnI GAANNNNTTC 1 cut(s) 1348
ZrmI AGTACT 1 cut(s) 713
Zsp2I ATGCAT 1 cut(s) 2764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.