MD14G1095600.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0010433
Physical Location & Seq
Forward (+)
14465411 .. 14477557
12147 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1095600.v1.1.491

Sequence Viewer

Length: 3504 bp
ATGGACAAGCCAGGCAAACATCAGGTTTCGTTGAGAGGAGCGAGCGCCAAGGAGATTACCAGAGACGCGCTGCTCGAGAAGGTCTCTCAGGAACGAGAGCTCCGTCAGTACGCCCGACGGGCCACGGCTGCTGCTCTCTTCATTCAGAGAGTGTGGAGGCGCTGCAGGGCAGCGAAGATTGCTGCGTTGGAGTTTCGAGAAGAGTGGGAGAAAGTAGTGGACCGGTATACTAAGTTAGCCTTCTCTGCAACATGGATTTCCAGCAATGTAGTGAGGCCTTTTCTTTTATTTGTTACTTGTTTATCTACTCGGCATAGGAGCATTCAAACCGCAGAAATGTGCTCTATGAAGACTTGCTTTCAGATTCTACTGGAAAGTGTGAATTCTACTGATTCAAAGAAGAACTATTGTTCCCTGGCAATTGGCACTCTTGAAGAAAGAAGAGTCTGGAGTTATCAGTCAAGGAAACTGCTTTCTCTCTGCATGTTTGTTCTTTCTGAGTGTGACACGTCCTGTGCACGGGGTCAAGATTTTGTTGCTCTCACCTCCCTAGCAATGCGTTTTGTTGTTGTCTTAACTGATCTCAAAGGATGGAAGAGCGTTGCTGAGCTTGACTGGCTAAGTGCAGATGCAGCAGTGAAGGATTTAGTTCGGTTTATGGGTGGTGGTGAAAGTGGTCTCTACTTAGCCATTAGAAGATACATTAGCACGTTGGATCCTCCGGGATCTTCACAGATAAGCAGTAATATACAAAGAGATGATAGTCTTCTGATTACTGCAAGTACAATAACTTTAGCTCTCAGGCCATTTCATCTAGCTAAGTTTGATTCAGATGGCCCTGGCATATTGGATGTCCATTATGTTGCTGAGAAGTACTGCATGTTTCTACTTACAATTCCTTGTATTACTCAACGCCTTCCAGCTGTGCTTATATCTGCTATGAGGCACAAGTCCATTCTCTCACCTTGTTTCCAGACATTATTGATTTTGAAAGAGAAAATATTAAAAGAGATGTTAGATGTGGATCAGTCAAAGGTTCATTTTCTTCCCAAGGTGATTCCCCCAGTTGGTTGGGCTCTTGCAAACATTATATGCCTTGCAACAGGGACAGAGAATGATTCCATTGATCCTGGAGGGTTCAGTCAAGATTTGGATTTTGTATCATATGTCTCTGCTGTTAACACTCTTGCAGAAAACTTATTGTCTAGGCTTGAGAATGTTGACTGTGTCAAGGATAACCAGAATCTCCAAAGTGATGCTGGAACACATGAGAAGTCCAATACAGTTTTATGTGAGGGTGAGACGGGGTCCTTTGAGATGTACTTGGATATGTTTAGGCCTATTTCCCAGCAGTGGCATCTTACAGATCTTTTGGCAACAATGAACAAAGTTGGTGATATTCAAGGGTCTGAGATTCTGACACCAAAAAAATGGGAACATTTAGGGAAGTTGGAACTGCTAGATGTTGTGAATTTATATTCTTACATGCTCAGAATATTTTCATTCTTGAACCCCGCAGTTGGGTCGTTGCCTGTCCTTAACATGCTGTCATTTACTCCTGGGTTTCTTGAGAATCTATGGAGAGCCTTGGAAACTTACCTTTACCCTGGGGATCGTCACACCGGTCATTATCGTTATGATTGTATCAGTAAAAATTCTGGTGGTGTAGAAAAGGATAAGGGTTTTGAGAGAAAACAAAAACACACCAACAATGATGGATTTAATAAGTGGGTCAGTGTTCTTCATAAAATTACTGGCAAGTCACAAGCAAGCGTTGATTGTACAAATTTGAATGATAGTCAACCTAAGCCTAGATCTGTTCATGAGGATTCATCTGACGTTTGGGATATAGAACCTGTGAGGCATGGCCCTCAAGGTATCTCAAGAGACATGTCATGTATGCTTCATCTTTTCTGTGCAAGCTATTCACACCTGCTTTTGATTCTCGATGACATAGAGTTTTACGAGAAACAGGTGCCATTCACACTGGAGCAACAACGGAAAATTGCGTCTGTTATCAATACATTAGTTTATAATGGATTTTCTCAAACTATTGGACAGCAGGGTAGACCCCTTATGGAATCTGCAATCAGATGCTTGCATTTAATGTATGAAAGGGATTGCAGGCACCAGTTCTGCCCTCCTATTTTGTGGCTTGCACCTGCTAGAAAGAACCGCCCTCCAAGTGCTGTAGCCGCCAGAACTCATGAAGTTTTTTCAGCAAATGTAGGGTCAGATGATGCTCAGGTTGTCCCAAGTATAGGCTCTCTTATAACTACAACTCCACATGTATTTCCATTTGAAGAAAGAGTTGAGATGTTTAGAGAGTTTATCAAGATGGACAAAGCCTCTAGAAAAATGGCTGGTGAAGTTGCTGGACCTGGTTCACGATCAGTTGAAATAGTAGTTCGCCGGGGTCATATTGTTGAAGATGGATTTCGGCAATTAAATTCCCTGGGTTCAAGGTTAAAATCATCAATCCATGTTTCATTTGTCAGTGAATGTGGCCTTCCGGAGGCTGGCCTGGACTATGGTGGATTATCCAAAGAGTTTTTGACTGATATATCAAAAGCAGCCTTTTCTCCCGATTATGGGCTATTCTCCCAAACCTCAACATCAGACGGACTTCTAATCCCTAACATGTCTGCAAGGTTCCTAGAGAATGGTATCCAGATGATTGAATTCCTTGGAAGAGTTGTTGGCAAAGCTCTTTATGAAGGAATTTTGCTAGACTACTCCTTTTCACATGTTTTTGTACAAAAGCTGTTAGGTCGGTATAGCTTTCTTGATGAATTGTCAACACTGGATCCAGAGCTTTACAAGAATCTCATATACGTGAAGCACTATGATGGTGATGTGGAGGAACTCTCTCTTGATTTTACAGTAACTGAAGAATCATTGGGGAAACGGCGTATCATTGAGCTTAAGCCTGGTGGAAAGGATGTTACTGTGACAAACAAGAACAGAATGCAGTACATTCATGGAATTGCTGATTATAAGCTTAACCGACAGATTTTTCCTTTCTCAAATGCGTTCTATAGAGGGTTAGCTGATGTCATATCACCATCCTGGCTAAAACTATTTAATGCAGGTGAATTTAATCAGTTGCTTTCGGGTGGAAACCATGACATTGACGTTGATGATTTAAGGAAGAACACAAAGTATACTGGTGGTTACTCTGAGGGGAGCCGGACGATTAAAATCTTTTGGGAGGTAATGGAAGGATTTGAACCTAAAGAACGTTGTATGCTTCTGAAATTTGTAACCAGTTGTTCTCGAGCTCCATTACTTGGATTCAAACACCTGCAGCCTACTTTCACAATTCATAAGGTTGCATGTGATATCCCTCTTTGGGCAACAATGAGAGGACAGGATGTTGAGCGGCTTCCATCAGCCTCGACTTGCTACAATACTCTGAAGCTTCCAACTTACAAACGTCCAAGCATTTTGAGAGACAAACTTCTATATGCAATCAGTTCCAATGCAGGATTTGAACTTTCTTGA

Protein Analysis

1168

Amino Acids

131.58

Weight (kDa)

7.51

Isoelectric Point (pI)

42.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HECT PF00632 854 - 1167 5.8e-96 HECT-domain (ubiquitin-transferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2034, 2273, 2997
AarI CACCTGC 4 cut(s) 1941, 2170, 3080, 3312
Acc36I ACCTGC 4 cut(s) 1941, 2170, 3080, 3312
AccB1I GGYRCC 2 cut(s) 1975, 2127
AccB7I CCANNNNNTGG 1 cut(s) 3290
AccBSI CCGCTC 1 cut(s) 3382
AccI GTMKAC 3 cut(s) 227, 2068, 3164
AccII CGCG 1 cut(s) 68
AccIII TCCGGA 1 cut(s) 2512
AciI CCGC 5 cut(s) 330, 1515, 2176, 2196, 3382
AclI AACGTT 1 cut(s) 3241
AclWI GGATC 8 cut(s) 710, 723, 733, 1032, 1121, 1620, 2801, 2814
AcsI RAATTY 9 cut(s) 382, 1471, 1654, 1786, 2449, 2681, 2721, 3095, 3257
AcuI CTGAAG 2 cut(s) 2910, 3437
AfaI GTAC 7 cut(s) 110, 784, 875, 1322, 1783, 2757, 2975
AflII CTTAAG 1 cut(s) 2924
AflIII ACRYGT 5 cut(s) 507, 1890, 2287, 2640, 2746
AgeI ACCGGT 2 cut(s) 222, 1622
AjiI CACGTC 1 cut(s) 510
AloI GAACNNNNNNTCC 4 cut(s) 84, 116, 395, 427
Alw21I GWGCWC 4 cut(s) 102, 344, 520, 3283
Alw26I GTCTC 7 cut(s) 57, 88, 683, 1174, 1295, 1881, 3447
Alw44I GTGCAC 1 cut(s) 516
AlwI GGATC 8 cut(s) 710, 723, 733, 1032, 1121, 1620, 2801, 2814
AlwNI CAGNNNCTG 1 cut(s) 2888
Ama87I CYCGRG 2 cut(s) 74, 3276
Aor13HI TCCGGA 1 cut(s) 2512
AoxI GGCC 8 cut(s) 120, 275, 803, 835, 1337, 1867, 2506, 2521
ApaLI GTGCAC 1 cut(s) 516
ApeKI GCWGC 9 cut(s) 70, 128, 131, 162, 170, 182, 632, 2573, 3307
ApoI RAATTY 9 cut(s) 382, 1471, 1654, 1786, 2449, 2681, 2721, 3095, 3257
ArsI GACNNNNNNTTYG 2 cut(s) 2216, 2248
AsiGI ACCGGT 2 cut(s) 222, 1622
Asp700I GAANNNNTTC 1 cut(s) 1498
AspLEI GCGC 3 cut(s) 47, 70, 162
AspS9I GGNCC 6 cut(s) 120, 220, 836, 1308, 1868, 2378
AsuC2I CCSGG 2 cut(s) 723, 2414
AvaI CYCGRG 2 cut(s) 74, 3276
AvaII GGWCC 3 cut(s) 220, 1308, 2378
BaeGI GKGCMC 1 cut(s) 520
BamHI GGATCC 2 cut(s) 715, 2806
BanI GGYRCC 2 cut(s) 1975, 2127
BanII GRGCYC 3 cut(s) 102, 1078, 3283
BbsI GAAGAC 2 cut(s) 356, 758
Bbv12I GWGCWC 4 cut(s) 102, 344, 520, 3283
BbvI GCAGC 9 cut(s) 57, 115, 118, 149, 169, 182, 644, 2585, 3319
BccI CCATC 8 cut(s) 585, 827, 1709, 2332, 2426, 2843, 3073, 3397
BceAI ACGGC 2 cut(s) 141, 2924
BcgI CGANNNNNNTGC 2 cut(s) 1506, 1540
BciVI GTATCC 1 cut(s) 2678
BcnI CCSGG 2 cut(s) 723, 2414
BcoDI GTCTC 7 cut(s) 57, 88, 683, 1174, 1295, 1881, 3447
BfaI CTAG 9 cut(s) 551, 815, 1206, 1460, 1812, 2166, 2352, 2657, 2729
BfmI CTRYAG 4 cut(s) 163, 2190, 3037, 3305
BfoI RGCGCY 2 cut(s) 48, 163
BfrI CTTAAG 1 cut(s) 2924
BfuAI ACCTGC 4 cut(s) 1941, 2170, 3080, 3312
BfuI GTATCC 1 cut(s) 2678
BglI GCCNNNNNGGC 1 cut(s) 119
BglII AGATCT 2 cut(s) 1366, 1814
BlpI GCTNAGC 1 cut(s) 606
BmcAI AGTACT 1 cut(s) 875
Bme18I GGWCC 3 cut(s) 220, 1308, 2378
BmeT110I CYCGRG 2 cut(s) 74, 3276
BmgBI CACGTC 1 cut(s) 510
BmgT120I GGNCC 6 cut(s) 120, 220, 836, 1308, 1868, 2378
BmiI GGNNCC 7 cut(s) 717, 1309, 1977, 2129, 2654, 2808, 3188
BmrI ACTGGG 1 cut(s) 1058
BmsI GCATC 5 cut(s) 619, 1246, 1366, 2084, 2230
BmuI ACTGGG 1 cut(s) 1058
BpiI GAAGAC 2 cut(s) 356, 758
BplI GAGNNNNNCTC 4 cut(s) 68, 100, 2852, 2884
BpmI CTGGAG 3 cut(s) 469, 1152, 2009
Bpu10I CCTNAGC 2 cut(s) 1806, 2244
Bpu1102I GCTNAGC 1 cut(s) 606
BpuEI CTTGAG 4 cut(s) 1232, 1589, 1857, 1867
BpuMI CCSGG 2 cut(s) 723, 2414
BsaAI YACGTR 1 cut(s) 2836
BsaBI GATNNNNATC 2 cut(s) 1023, 3200
BsaI GGTCTC 2 cut(s) 88, 683
BsaWI WCCGGW 3 cut(s) 222, 1622, 2512
BsaXI ACNNNNNCTCC 4 cut(s) 84, 114, 2266, 2296
Bse118I RCCGGY 2 cut(s) 222, 1622
Bse1I ACTGG 9 cut(s) 375, 620, 1064, 1759, 1992, 2131, 2808, 3172, 3267
Bse3DI GCAATG 2 cut(s) 271, 561
Bse8I GATNNNNATC 2 cut(s) 1023, 3200
BseAI TCCGGA 1 cut(s) 2512
BseGI GGATG 5 cut(s) 596, 856, 2947, 3065, 3379
BseJI GATNNNNATC 2 cut(s) 1023, 3200
BseMI GCAATG 2 cut(s) 271, 561
BseMII CTCAG 9 cut(s) 101, 489, 597, 814, 858, 1401, 1504, 2258, 3171
BseNI ACTGG 9 cut(s) 375, 620, 1064, 1759, 1992, 2131, 2808, 3172, 3267
BseRI GAGGAG 1 cut(s) 51
BseSI GKGCMC 1 cut(s) 520
BseXI GCAGC 9 cut(s) 57, 115, 118, 149, 169, 182, 644, 2585, 3319
BseYI CCCAGC 1 cut(s) 1347
BsgI GTGCAG 1 cut(s) 645
Bsh1236I CGCG 1 cut(s) 68
BshFI GGCC 8 cut(s) 122, 277, 805, 837, 1339, 1869, 2508, 2523
BshNI GGYRCC 2 cut(s) 1975, 2127
BshTI ACCGGT 2 cut(s) 222, 1622
BsiHKAI GWGCWC 4 cut(s) 102, 344, 520, 3283
BsiHKCI CYCGRG 2 cut(s) 74, 3276
BsiSI CCGG 6 cut(s) 223, 722, 1623, 2413, 2513, 3190
BslFI GGGAC 2 cut(s) 1120, 2237
BsmAI GTCTC 7 cut(s) 57, 88, 683, 1174, 1295, 1881, 3447
BsmBI CGTCTC 2 cut(s) 57, 1295
BsmFI GGGAC 2 cut(s) 1120, 2237
BsmI GAATGC 2 cut(s) 321, 2973
BsnI GGCC 8 cut(s) 122, 277, 805, 837, 1339, 1869, 2508, 2523
Bso31I GGTCTC 2 cut(s) 88, 683
BsoBI CYCGRG 2 cut(s) 74, 3276
Bsp1286I GDGCHC 5 cut(s) 102, 344, 520, 1078, 3283
Bsp13I TCCGGA 1 cut(s) 2512
Bsp1407I TGTACA 2 cut(s) 1781, 2755
Bsp1720I GCTNAGC 1 cut(s) 606
BspACI CCGC 5 cut(s) 330, 1515, 2176, 2196, 3382
BspANI GGCC 8 cut(s) 122, 277, 805, 837, 1339, 1869, 2508, 2523
BspCNI CTCAG 9 cut(s) 100, 490, 598, 813, 859, 1402, 1503, 2257, 3172
BspEI TCCGGA 1 cut(s) 2512
BspFNI CGCG 1 cut(s) 68
BspHI TCATGA 2 cut(s) 1822, 2206
BspLI GGNNCC 7 cut(s) 717, 1309, 1977, 2129, 2654, 2808, 3188
BspMAI CTGCAG 2 cut(s) 167, 3309
BspMI ACCTGC 4 cut(s) 1941, 2170, 3080, 3312
BspPI GGATC 8 cut(s) 710, 723, 733, 1032, 1121, 1620, 2801, 2814
BspQI GCTCTTC 1 cut(s) 590
BspT107I GGYRCC 2 cut(s) 1975, 2127
BspTI CTTAAG 1 cut(s) 2924
BspTNI GGTCTC 2 cut(s) 88, 683
BsrBI CCGCTC 1 cut(s) 3382
BsrDI GCAATG 2 cut(s) 271, 561
BsrFI RCCGGY 2 cut(s) 222, 1622
BsrGI TGTACA 2 cut(s) 1781, 2755
BsrI ACTGG 9 cut(s) 375, 620, 1064, 1759, 1992, 2131, 2808, 3172, 3267
BssAI RCCGGY 2 cut(s) 222, 1622
BssNAI GTATAC 2 cut(s) 228, 3165
BssT1I CCWWGG 4 cut(s) 48, 1050, 1587, 2686
Bst1107I GTATAC 2 cut(s) 228, 3165
Bst4CI ACNGT 4 cut(s) 1226, 1285, 2884, 2950
Bst6I CTCTTC 5 cut(s) 143, 195, 436, 590, 2686
BstAFI CTTAAG 1 cut(s) 2924
BstAUI TGTACA 2 cut(s) 1781, 2755
BstBAI YACGTR 1 cut(s) 2836
BstC8I GCNNGC 7 cut(s) 43, 1771, 1921, 2099, 2126, 2157, 2521
BstDSI CCRYGG 1 cut(s) 123
BstENI CCTNNNNNAGG 1 cut(s) 2513
BstF5I GGATG 5 cut(s) 596, 856, 2947, 3065, 3379
BstFNI CGCG 1 cut(s) 68
BstH2I RGCGCY 2 cut(s) 48, 163
BstHHI GCGC 3 cut(s) 47, 70, 162
BstMAI GTCTC 7 cut(s) 57, 88, 683, 1174, 1295, 1881, 3447
BstMWI GCNNNNNNNGC 7 cut(s) 119, 128, 179, 245, 616, 632, 2195
BstNSI RCATGY 9 cut(s) 487, 883, 1489, 1546, 1894, 2291, 2644, 2750, 3339
BstSFI CTRYAG 4 cut(s) 163, 2190, 3037, 3305
BstSLI GKGCMC 1 cut(s) 520
BstUI CGCG 1 cut(s) 68
BstV1I GCAGC 9 cut(s) 57, 115, 118, 149, 169, 182, 644, 2585, 3319
BstV2I GAAGAC 2 cut(s) 356, 758
BstX2I RGATCY 5 cut(s) 715, 725, 1366, 1814, 2806
BstXI CCANNNNNNTGG 2 cut(s) 1071, 1431
BstYI RGATCY 5 cut(s) 715, 725, 1366, 1814, 2806
BstZ17I GTATAC 2 cut(s) 228, 3165
BsuI GTATCC 1 cut(s) 2678
BsuRI GGCC 8 cut(s) 122, 277, 805, 837, 1339, 1869, 2508, 2523
BtgI CCRYGG 1 cut(s) 123
BtrI CACGTC 1 cut(s) 510
BtsCI GGATG 5 cut(s) 596, 856, 2947, 3065, 3379
BtsI GCAGTG 2 cut(s) 642, 1358
BtsIMutI CAGTG 6 cut(s) 642, 1358, 1741, 1985, 2503, 2801
BveI ACCTGC 4 cut(s) 1941, 2170, 3080, 3312
Cac8I GCNNGC 7 cut(s) 43, 1771, 1921, 2099, 2126, 2157, 2521
CaiI CAGNNNCTG 1 cut(s) 2888
CciI TCATGA 2 cut(s) 1822, 2206
CfoI GCGC 3 cut(s) 47, 70, 162
Cfr10I RCCGGY 2 cut(s) 222, 1622
Cfr13I GGNCC 6 cut(s) 120, 220, 836, 1308, 1868, 2378
CseI GACGC 2 cut(s) 74, 1998
CsiI ACCWGGT 1 cut(s) 2380
Csp6I GTAC 7 cut(s) 109, 783, 874, 1321, 1782, 2756, 2974
CspAI ACCGGT 2 cut(s) 222, 1622
CviQI GTAC 7 cut(s) 109, 783, 874, 1321, 1782, 2756, 2974
Eam1104I CTCTTC 5 cut(s) 143, 195, 436, 590, 2686
EarI CTCTTC 5 cut(s) 143, 195, 436, 590, 2686
Ecl136II GAGCTC 2 cut(s) 100, 3281
Eco130I CCWWGG 4 cut(s) 48, 1050, 1587, 2686
Eco147I AGGCCT 2 cut(s) 277, 1339
Eco24I GRGCYC 3 cut(s) 102, 1078, 3283
Eco31I GGTCTC 2 cut(s) 88, 683
Eco32I GATATC 1 cut(s) 3343
Eco47I GGWCC 3 cut(s) 220, 1308, 2378
Eco53kI GAGCTC 2 cut(s) 100, 3281
Eco57I CTGAAG 2 cut(s) 2910, 3437
Eco88I CYCGRG 2 cut(s) 74, 3276
EcoICRI GAGCTC 2 cut(s) 100, 3281
EcoNI CCTNNNNNAGG 1 cut(s) 2513
EcoO109I RGGNCCY 1 cut(s) 1308
EcoRI GAATTC 2 cut(s) 382, 2681
EcoRV GATATC 1 cut(s) 3343
EcoT14I CCWWGG 4 cut(s) 48, 1050, 1587, 2686
EcoT38I GRGCYC 3 cut(s) 102, 1078, 3283
ErhI CCWWGG 4 cut(s) 48, 1050, 1587, 2686
Esp3I CGTCTC 2 cut(s) 57, 1295
FalI AAGNNNNNCTT 4 cut(s) 224, 256, 341, 373
FaqI GGGAC 2 cut(s) 1120, 2237
FauI CCCGC 1 cut(s) 1522
FauNDI CATATG 1 cut(s) 1165
FblI GTMKAC 3 cut(s) 227, 2068, 3164
FokI GGATG 5 cut(s) 603, 863, 2954, 3052, 3386
FriOI GRGCYC 3 cut(s) 102, 1078, 3283
FspBI CTAG 9 cut(s) 551, 815, 1206, 1460, 1812, 2166, 2352, 2657, 2729
GlaI GCGC 3 cut(s) 46, 69, 161
GsaI CCCAGC 1 cut(s) 1351
GsuI CTGGAG 3 cut(s) 469, 1152, 2009
HaeII RGCGCY 2 cut(s) 48, 163
HaeIII GGCC 8 cut(s) 122, 277, 805, 837, 1339, 1869, 2508, 2523
HapII CCGG 6 cut(s) 223, 722, 1623, 2413, 2513, 3190
HgaI GACGC 2 cut(s) 74, 1998
HhaI GCGC 3 cut(s) 47, 70, 162
Hin6I GCGC 3 cut(s) 45, 68, 160
HinP1I GCGC 3 cut(s) 45, 68, 160
HincII GTYRAC 4 cut(s) 1180, 1222, 1802, 2799
HindII GTYRAC 4 cut(s) 1180, 1222, 1802, 2799
HindIII AAGCTT 2 cut(s) 2999, 3419
HpaI GTTAAC 1 cut(s) 1180
HpaII CCGG 6 cut(s) 223, 722, 1623, 2413, 2513, 3190
Hpy99I CGWCG 1 cut(s) 120
HpyAV CCTTC 7 cut(s) 73, 250, 634, 926, 2519, 2711, 3215
HpyCH4III ACNGT 4 cut(s) 1226, 1285, 2884, 2950
HpyCH4IV ACGT 7 cut(s) 509, 710, 1839, 2835, 3135, 3241, 3436
HpyF10VI GCNNNNNNNGC 7 cut(s) 119, 128, 179, 245, 616, 632, 2195
HpySE526I ACGT 7 cut(s) 509, 710, 1839, 2835, 3135, 3241, 3436
HspAI GCGC 3 cut(s) 45, 68, 160
Kpn2I TCCGGA 1 cut(s) 2512
KspAI GTTAAC 1 cut(s) 1180
LguI GCTCTTC 1 cut(s) 590
LmnI GCTCC 6 cut(s) 38, 105, 318, 1990, 3186, 3286
Lsp1109I GCAGC 9 cut(s) 57, 115, 118, 149, 169, 182, 644, 2585, 3319
LweI GCATC 5 cut(s) 619, 1246, 1366, 2084, 2230
MabI ACCWGGT 1 cut(s) 2380
MaeI CTAG 9 cut(s) 551, 815, 1206, 1460, 1812, 2166, 2352, 2657, 2729
MaeII ACGT 7 cut(s) 509, 710, 1839, 2835, 3135, 3241, 3436
MaeIII GTNAC 9 cut(s) 292, 503, 1616, 1761, 2884, 2944, 2950, 3173, 3262
MbiI CCGCTC 1 cut(s) 3382
MfeI CAATTG 1 cut(s) 420
MflI RGATCY 5 cut(s) 715, 725, 1366, 1814, 2806
MhlI GDGCHC 5 cut(s) 102, 344, 520, 1078, 3283
MlyI GAGTC 1 cut(s) 453
MmeI TCCRAC 4 cut(s) 168, 693, 1431, 3449
MroI TCCGGA 1 cut(s) 2512
MroXI GAANNNNTTC 1 cut(s) 1498
MslI CAYNNNNRTG 2 cut(s) 2834, 2847
MspA1I CMGCKG 1 cut(s) 923
MspCI CTTAAG 1 cut(s) 2924
MspI CCGG 6 cut(s) 223, 722, 1623, 2413, 2513, 3190
MunI CAATTG 1 cut(s) 420
Mva1269I GAATGC 2 cut(s) 321, 2973
MvnI CGCG 1 cut(s) 68
MwoI GCNNNNNNNGC 7 cut(s) 119, 128, 179, 245, 616, 632, 2195
NciI CCSGG 2 cut(s) 723, 2414
NdeI CATATG 1 cut(s) 1165
NlaIV GGNNCC 7 cut(s) 717, 1309, 1977, 2129, 2654, 2808, 3188
NmeAIII GCCGAG 1 cut(s) 289
NmuCI GTSAC 4 cut(s) 503, 1616, 1761, 2950
NspI RCATGY 9 cut(s) 487, 883, 1489, 1546, 1894, 2291, 2644, 2750, 3339
PaeR7I CTCGAG 2 cut(s) 74, 3276
PagI TCATGA 2 cut(s) 1822, 2206
PaqCI CACCTGC 4 cut(s) 1941, 2170, 3080, 3312
PasI CCCWGGG 2 cut(s) 1607, 2455
PceI AGGCCT 2 cut(s) 277, 1339
PciI ACATGT 4 cut(s) 1890, 2287, 2640, 2746
PciSI GCTCTTC 1 cut(s) 590
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 100
PctI GAATGC 2 cut(s) 321, 2973
PdmI GAANNNNTTC 1 cut(s) 1498
PflFI GACNNNGTC 2 cut(s) 1226, 1306
PflMI CCANNNNNTGG 1 cut(s) 3290
PfoI TCCNGGA 2 cut(s) 721, 1129
PinAI ACCGGT 2 cut(s) 222, 1622
PleI GAGTC 1 cut(s) 452
PpsI GAGTC 1 cut(s) 452
Ppu21I YACGTR 1 cut(s) 2836
PpuMI RGGWCCY 1 cut(s) 1308
PscI ACATGT 4 cut(s) 1890, 2287, 2640, 2746
PsiI TTATAA 3 cut(s) 2034, 2273, 2997
Psp124BI GAGCTC 2 cut(s) 102, 3283
Psp1406I AACGTT 1 cut(s) 3241
Psp5II RGGWCCY 1 cut(s) 1308
PspFI CCCAGC 1 cut(s) 1347
PspN4I GGNNCC 7 cut(s) 717, 1309, 1977, 2129, 2654, 2808, 3188
PspPI GGNCC 6 cut(s) 120, 220, 836, 1308, 1868, 2378
PspPPI RGGWCCY 1 cut(s) 1308
PstI CTGCAG 2 cut(s) 167, 3309
PstNI CAGNNNCTG 1 cut(s) 2888
PsuI RGATCY 5 cut(s) 715, 725, 1366, 1814, 2806
PsyI GACNNNGTC 2 cut(s) 1226, 1306
PvuII CAGCTG 1 cut(s) 923
RsaI GTAC 7 cut(s) 110, 784, 875, 1322, 1783, 2757, 2975
RsaNI GTAC 7 cut(s) 109, 783, 874, 1321, 1782, 2756, 2974
RseI CAYNNNNRTG 2 cut(s) 2834, 2847
SacI GAGCTC 2 cut(s) 102, 3283
SapI GCTCTTC 1 cut(s) 590
Sau96I GGNCC 6 cut(s) 120, 220, 836, 1308, 1868, 2378
ScaI AGTACT 1 cut(s) 875
SchI GAGTC 1 cut(s) 453
SduI GDGCHC 5 cut(s) 102, 344, 520, 1078, 3283
SexAI ACCWGGT 1 cut(s) 2380
SfaNI GCATC 5 cut(s) 619, 1246, 1366, 2084, 2230
SfcI CTRYAG 4 cut(s) 163, 2190, 3037, 3305
Sfr274I CTCGAG 2 cut(s) 74, 3276
SinI GGWCC 3 cut(s) 220, 1308, 2378
SlaI CTCGAG 2 cut(s) 74, 3276
SmiMI CAYNNNNRTG 2 cut(s) 2834, 2847
SmlI CTYRAG 7 cut(s) 74, 1211, 1568, 1872, 1882, 2924, 3276
SmoI CTYRAG 7 cut(s) 74, 1211, 1568, 1872, 1882, 2924, 3276
SseBI AGGCCT 2 cut(s) 277, 1339
SsiI CCGC 5 cut(s) 330, 1515, 2176, 2196, 3382
SspI AATATT 2 cut(s) 1002, 1497
SspMI CTAG 9 cut(s) 551, 815, 1206, 1460, 1812, 2166, 2352, 2657, 2729
SstI GAGCTC 2 cut(s) 102, 3283
StuI AGGCCT 2 cut(s) 277, 1339
StyI CCWWGG 4 cut(s) 48, 1050, 1587, 2686
TaaI ACNGT 4 cut(s) 1226, 1285, 2884, 2950
TaiI ACGT 7 cut(s) 512, 713, 1842, 2838, 3138, 3244, 3439
TaqI TCGA 5 cut(s) 75, 196, 1947, 3277, 3398
TatI WGTACW 6 cut(s) 782, 873, 1320, 1781, 2755, 2973
TauI GCSGC 2 cut(s) 2198, 3385
TscAI CASTG 6 cut(s) 642, 1358, 1741, 1992, 2503, 2808
TseFI GTSAC 4 cut(s) 503, 1616, 1761, 2950
TseI GCWGC 9 cut(s) 70, 128, 131, 162, 170, 182, 632, 2573, 3307
Tsp45I GTSAC 4 cut(s) 503, 1616, 1761, 2950
TspGWI ACGGA 3 cut(s) 92, 2014, 2637
TspRI CASTG 6 cut(s) 642, 1358, 1741, 1992, 2503, 2808
Tth111I GACNNNGTC 2 cut(s) 1226, 1306
Van91I CCANNNNNTGG 1 cut(s) 3290
Vha464I CTTAAG 1 cut(s) 2924
VneI GTGCAC 1 cut(s) 516
VpaK11BI GGWCC 3 cut(s) 220, 1308, 2378
XagI CCTNNNNNAGG 1 cut(s) 2513
XapI RAATTY 9 cut(s) 382, 1471, 1654, 1786, 2449, 2681, 2721, 3095, 3257
XbaI TCTAGA 1 cut(s) 2351
XceI RCATGY 9 cut(s) 487, 883, 1489, 1546, 1894, 2291, 2644, 2750, 3339
XcmI CCANNNNNNNNNTGG 1 cut(s) 1256
XhoI CTCGAG 2 cut(s) 74, 3276
XmiI GTMKAC 3 cut(s) 227, 2068, 3164
XmnI GAANNNNTTC 1 cut(s) 1498
XspI CTAG 9 cut(s) 551, 815, 1206, 1460, 1812, 2166, 2352, 2657, 2729
ZrmI AGTACT 1 cut(s) 875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.