MD12G1101300.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010433
Physical Location & Seq
Forward (+)
15878725 .. 15898634
19910 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1101300.v1.1.491

Sequence Viewer

Length: 3492 bp
ATGGACAAGCGAGGCAAACATCAGGTTTCGTTGAGAGGAGCGAGCGCCAAGGAGATTACCAGAGACGCGCTTCTCGAGAAGGTCTCTCAGGAACGAGAGCTCCGTCAGTACGCCCGACGGGCCACGGTTGCTGCGCTCTTCATTCAGAGAGAGTGGAGGCGCTACAGGGCCGTGAAGATGGCCGCGTTGGAGGTTCGAGGAGAGTGGGAGAAAGTGGTGGAACAGTATGCTGAGTTAGCCTTCACTGCAACATGGATTTCCAGCAATGTAGTGAGGCCTTTTCTTTTCTTTATTACTTGTTTATCTACTTGGCATAGCAACATTCAAACTACAGAAATGTGCTCTATGAAGACCTGCTTTCAGATTCTACTGGAAAGCGTGAATTCTACTGATTCAAAGAAGAACTTTTGTTCATTGGCAATCGGCACTCTTGAAGAGAGTAGAATCTGGAGTTATCAGTCAAGGAAGCTGATTTCTCTCTGCATGTTTGTTCTTTCTAAGTGTGACACGTCCCGTGCACGGGATCAAGTTATTGTTGCTCTCACCTCCTTAGCAATGCGTTTTGTTGTTGTCTTAACTGATTTCAAAGGATGGAAGAGCGTTGCTGAGCATGACTGCCAGAGTGCAGATGCGGCAGTGAAGTATTTAGTTCGATTTATGGGGACTGGTGAAAGTGGTCTTTACCAATCTATTAGAAGATACATTAGTACATTGGATGCTCCTGGATTTTCACGGATAAGCAGTAACATACAAAGAGATGATAGTTTTCTGATTACAGCAAGTACAATAACTTTAGCTCTCAGGCCATTTCATCTAGCAAAGTTTGATTCAGATGGCCCTGGCTCATTGGATGTCCATTATGTTGCCGAGAAGTACTGCGTGTTTCTACTTACAATTCCTTGTCTTACCCAACGCCTTCCAGCTGTGCTTATATCTGCTATGAGGCACAAGTCCATTCTCTCACCTTGTTTCCAGACATTACTGATTTTGAAAGACAAAATACTAAAGGAGATGTTAGATGTGGATCAATCAAAGGTTCATTTTCTTCCGAAGGTGATTCCCCCAGTTGGTTGGGCTCTTGCAAACATTATATGCCTAGCAACAGTGACAGAGAGTGATTCCGTTGATCCTGGAGGGTTCAATCAAGATTTGGACTCTGTCTCATATGTCTGTGCTGTTAACACTCTTGCAGAAACCTTATTGTCTATGCTTGAGAATGTTGACTGTGTCAAGGATAAGCAGGCTCTCCAAAGTGATGTTGAAACCCATGAGAAGCCCAATACAGTTTTATGTGAGGGTGAGACGGGTTCCTTTAAGATGTACTTGGATATGTTTAGGCCTATTTCCCAGCAGTGGCATCTTACAGATCTCTTGGCAACAATGAATAAAGTTGGTGATATTCAAGGGTCTGAGACTCTGACACCAAAAAAAATTGAACGTTTAGGGAAGTTGGAACTGCTAGATATTGTGCATTTATATTCTTACATGCTCAGAATATTTTCATTCTTGAACCCCACAGTTGGGTCTTTTCCTGTCCTTAACATGCTCTCTTTTACTCCTGGGTTTCTTGTGAATCTGTGGAGAGCCTTGGAAACTTACCTTTTTCCTGGGGATGGTCACACTGCTCCTGAGCATTATGATTGTATCACTAAAAAGTCTGGTGGTGTAAAAAAAGATGAGGCTTTTGAGAGAAAACTAAAACACACCAACAATGATGGAGTTAATAAGTGGGTCACTGTTCTTCATAAAATTACTGGCAAGTCGCAAGCAGGCATTGATAATACAAATTTGAGTGATAGTCAACCTAATCCTAGATCTGTTCATGAGGATTCTTCTGATGTTTGGGATATAGAACCTGTGAGGCATGGCCCTCAAGGTATTTCAAGAGACATGTCATGTATGCTTTATCTTTTCTGTGCAAGTTATTCGCACCTGCTTTTGATTCTTGATGATATAGAGTTTTATGAGAAACAGGTGCCATTCACACTGGAGCAACAACGGAAAATTGCATCTGTTCTCAATGCATTAGTTTATAACGGATTTTCTCAAACTATTGGACAGCAGGATAGACCCCTTATGGAATCTGCAATCAGATGCTTGCATTTAATGTATGAAAGGGATTGCAGGCACCAGTTCTGCCCCTCTGTTTTGTGGCTTGCACCTGCTAGAAAGAACCGCCCTCCAATTGCTGTAGCTGCCAGAACTCATGAAGTTTTCTCAACAAATGTAGGATCAGATGATGCCCCAGTCATTCCAAGTATAGGCTCTGTTATAACTACAACTCCACATGTATTTCCATTTGAAGAAAGAGTTGAGATGTTTAGAGAGTTTATCAAGATGGACAAAGCCTCCAGAAAAATGGCTGGTGACGTTGCTGGACCTGGTTCACGATCAGTTGAGATAGTAGTTCGCCGTGGTCATATTGTTGAAGATGGATTTCAGCAATTAAATTCCCTGGGTTCAAGGTTAAAGTCATCAATCCATGTTTCATTTGTCAGTGAATGTGGCCTTCCGGAGGCTGGCCTGGACTATGGTGGATTATCTAAAGAGTTTTTGACAGATATATCAAAAGCAGCCTTTGCTCCTGATTATGGGCTATTTTCCCAAACCTCAACATCAGACCGACTTCTAATGCCTAATGCGTCCGCAAGGTTCCTAGAGAATGGTATCCAGATGATTGAATTCCTTGGAAGAGTTCTGTTAGGTCGGTATAGCTTTCTTGATGAACTATCAACACTGGATCCAGAGCTTTACAAGAATCTCATGTATGTGAAGCACTATGATGGTGACGTGGAGGAACTCTGTCTTGATTTTACAGTAACTGAAGAATCATTTGGGAAACGGCATGTCATTGAGCTTAAGCCTGGTGGAAAGGATACTACTGTGACAAACAAGAACAAAATGCAATACATTCATGCAATTGCTGATTATAAGCTTAACCGACAGATATTTCCTTTTTCAAATGCATTCTATAGAGGGTTAGCTGATCTCATATCACCATCCTGGCTAAAATTGTTTAATGCAGGTGAATTTAATCAGTTGCTTTCAGGTGGAAACCATGACATTGATGTGGATGATTTAAGGAAGAACACAAGGTACACTGGTGGTTACTCTGACGGGAGCCGAACGATTAAAATTTTTTGGGAGGTAATGAAAGGTTTTGAACCTAAAGAACGTTGTATGCTTCTAAAATTCGTCACCAGTTGTTCTCGAGCTCCATTACTTGGATTCAAGCACCTGCAACCTACTTTTACAATCCATAAGGTTGCATGTGATATCCCTGTCTGGGATACAATGAGAGGACATGATGTTGAGCGGCTTCCATCAGCCTCAACTTGCTATAATACTCTGAAGCTTCCAACTTACAAACGTCCAAGCACTTTGAGAGACAAACTTCTATATGCAATCAGATTCATAATCATATCCAGAAGAGGCTGGAAGATATTGAAGACCACAAGCTTGAGGCCTGGAAGCCTATTCTTCAAATCTTGA

Protein Analysis

1164

Amino Acids

131.57

Weight (kDa)

8.36

Isoelectric Point (pI)

42.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HECT PF00632 892 - 1137 1e-75 HECT-domain (ubiquitin-transferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2034, 2273, 2931
AarI CACCTGC 4 cut(s) 1941, 2170, 3014, 3246
Acc36I ACCTGC 5 cut(s) 362, 1941, 2170, 3014, 3246
AccB1I GGYRCC 2 cut(s) 1975, 2127
AccB7I CCANNNNNTGG 1 cut(s) 3224
AccBSI CCGCTC 1 cut(s) 3316
AccII CGCG 2 cut(s) 68, 185
AccIII TCCGGA 1 cut(s) 2512
AciI CCGC 5 cut(s) 183, 632, 2176, 2646, 3316
AclI AACGTT 2 cut(s) 1438, 3175
AclWI GGATC 6 cut(s) 531, 1032, 1121, 2239, 2735, 2748
AcoI YGGCCR 1 cut(s) 180
AcsI RAATTY 7 cut(s) 382, 1786, 2449, 2681, 3029, 3135, 3191
AcuI CTGAAG 2 cut(s) 2844, 3371
AfaI GTAC 6 cut(s) 110, 709, 784, 875, 1322, 3098
AflII CTTAAG 1 cut(s) 2858
AflIII ACRYGT 3 cut(s) 507, 1890, 2287
AjiI CACGTC 2 cut(s) 510, 2791
AjuI GAANNNNNNNTTGG 2 cut(s) 2817, 2849
AloI GAACNNNNNNTCC 2 cut(s) 84, 116
Alw21I GWGCWC 4 cut(s) 102, 344, 520, 3217
Alw26I GTCTC 7 cut(s) 57, 88, 1165, 1295, 1406, 1881, 3381
Alw44I GTGCAC 1 cut(s) 516
AlwI GGATC 6 cut(s) 531, 1032, 1121, 2239, 2735, 2748
AlwNI CAGNNNCTG 1 cut(s) 2822
Ama87I CYCGRG 2 cut(s) 74, 3210
Aor13HI TCCGGA 1 cut(s) 2512
ApaLI GTGCAC 1 cut(s) 516
ApeKI GCWGC 3 cut(s) 131, 2195, 2573
ApoI RAATTY 7 cut(s) 382, 1786, 2449, 2681, 3029, 3135, 3191
Asp700I GAANNNNTTC 1 cut(s) 1498
AspLEI GCGC 4 cut(s) 47, 70, 136, 162
AspS9I GGNCC 5 cut(s) 120, 168, 836, 1868, 2378
AvaI CYCGRG 2 cut(s) 74, 3210
AvaII GGWCC 1 cut(s) 2378
BaeGI GKGCMC 1 cut(s) 520
BaeI ACNNNNGTAYC 6 cut(s) 691, 724, 2868, 2901, 3088, 3121
BamHI GGATCC 1 cut(s) 2740
BanI GGYRCC 2 cut(s) 1975, 2127
BanII GRGCYC 3 cut(s) 102, 1078, 3217
BbsI GAAGAC 2 cut(s) 356, 3455
Bbv12I GWGCWC 4 cut(s) 102, 344, 520, 3217
BbvI GCAGC 3 cut(s) 118, 2182, 2585
BceAI ACGGC 3 cut(s) 155, 2397, 2858
BcgI CGANNNNNNTGC 2 cut(s) 1908, 1942
BciVI GTATCC 3 cut(s) 2678, 2869, 3283
BcoDI GTCTC 7 cut(s) 57, 88, 1165, 1295, 1406, 1881, 3381
BfaI CTAG 6 cut(s) 815, 1097, 1460, 1812, 2166, 2657
BfmI CTRYAG 4 cut(s) 163, 330, 2190, 2971
BfoI RGCGCY 2 cut(s) 48, 163
BfrI CTTAAG 1 cut(s) 2858
BfuAI ACCTGC 5 cut(s) 362, 1941, 2170, 3014, 3246
BfuI GTATCC 3 cut(s) 2678, 2869, 3283
BglI GCCNNNNNGGC 1 cut(s) 119
BglII AGATCT 2 cut(s) 1366, 1814
BisI GCNGC 6 cut(s) 132, 183, 633, 2196, 2574, 3317
BlpI GCTNAGC 1 cut(s) 606
BlsI GCNGC 6 cut(s) 133, 184, 634, 2197, 2575, 3318
BmcAI AGTACT 1 cut(s) 875
Bme18I GGWCC 1 cut(s) 2378
BmeT110I CYCGRG 2 cut(s) 74, 3210
BmgBI CACGTC 2 cut(s) 510, 2791
BmgT120I GGNCC 5 cut(s) 120, 168, 836, 1868, 2378
BmiI GGNNCC 6 cut(s) 1309, 1977, 2129, 2654, 2742, 3122
BmrI ACTGGG 2 cut(s) 1058, 2240
BmsI GCATC 6 cut(s) 619, 706, 1366, 2018, 2084, 2230
BmuI ACTGGG 2 cut(s) 1058, 2240
BpiI GAAGAC 2 cut(s) 356, 3455
BplI GAGNNNNNCTC 2 cut(s) 68, 100
BpmI CTGGAG 4 cut(s) 469, 1152, 2009, 2335
Bpu10I CCTNAGC 2 cut(s) 550, 1629
Bpu1102I GCTNAGC 1 cut(s) 606
BpuEI CTTGAG 3 cut(s) 1232, 1857, 3481
BsaBI GATNNNNATC 1 cut(s) 1023
BsaI GGTCTC 1 cut(s) 88
BsaWI WCCGGW 1 cut(s) 2512
BsaXI ACNNNNNCTCC 6 cut(s) 84, 114, 2266, 2296, 2333, 2363
Bse3DI GCAATG 2 cut(s) 271, 561
Bse8I GATNNNNATC 1 cut(s) 1023
BseAI TCCGGA 1 cut(s) 2512
BseGI GGATG 6 cut(s) 596, 721, 856, 1618, 2999, 3079
BseJI GATNNNNATC 1 cut(s) 1023
BseMI GCAATG 2 cut(s) 271, 561
BseMII CTCAG 7 cut(s) 101, 222, 597, 814, 1401, 1504, 1620
BseRI GAGGAG 2 cut(s) 51, 213
BseSI GKGCMC 1 cut(s) 520
BseXI GCAGC 3 cut(s) 118, 2182, 2585
BseYI CCCAGC 1 cut(s) 1347
BsgI GTGCAG 1 cut(s) 645
Bsh1236I CGCG 2 cut(s) 68, 185
BshNI GGYRCC 2 cut(s) 1975, 2127
BsiHKAI GWGCWC 4 cut(s) 102, 344, 520, 3217
BsiHKCI CYCGRG 2 cut(s) 74, 3210
BsiSI CCGG 1 cut(s) 2513
BslFI GGGAC 2 cut(s) 496, 676
BsmAI GTCTC 7 cut(s) 57, 88, 1165, 1295, 1406, 1881, 3381
BsmBI CGTCTC 2 cut(s) 57, 1295
BsmFI GGGAC 2 cut(s) 496, 676
BsmI GAATGC 1 cut(s) 2966
Bso31I GGTCTC 1 cut(s) 88
BsoBI CYCGRG 2 cut(s) 74, 3210
Bsp1286I GDGCHC 5 cut(s) 102, 344, 520, 1078, 3217
Bsp13I TCCGGA 1 cut(s) 2512
Bsp143I GATC 9 cut(s) 523, 1024, 1126, 1366, 1814, 2231, 2390, 2740, 2986
Bsp1720I GCTNAGC 1 cut(s) 606
BspACI CCGC 5 cut(s) 183, 632, 2176, 2646, 3316
BspCNI CTCAG 7 cut(s) 100, 223, 598, 813, 1402, 1503, 1621
BspEI TCCGGA 1 cut(s) 2512
BspFNI CGCG 2 cut(s) 68, 185
BspHI TCATGA 2 cut(s) 1822, 2206
BspLI GGNNCC 6 cut(s) 1309, 1977, 2129, 2654, 2742, 3122
BspMI ACCTGC 5 cut(s) 362, 1941, 2170, 3014, 3246
BspPI GGATC 6 cut(s) 531, 1032, 1121, 2239, 2735, 2748
BspQI GCTCTTC 2 cut(s) 143, 590
BspT107I GGYRCC 2 cut(s) 1975, 2127
BspTI CTTAAG 1 cut(s) 2858
BspTNI GGTCTC 1 cut(s) 88
BsrBI CCGCTC 1 cut(s) 3316
BsrDI GCAATG 2 cut(s) 271, 561
BssMI GATC 9 cut(s) 523, 1024, 1126, 1366, 1814, 2231, 2390, 2740, 2986
BssT1I CCWWGG 3 cut(s) 48, 1587, 2686
Bst4CI ACNGT 9 cut(s) 127, 225, 1105, 1226, 1285, 1519, 1738, 2818, 2884
Bst6I CTCTTC 5 cut(s) 143, 429, 590, 2686, 3424
BstAFI CTTAAG 1 cut(s) 2858
BstAPI GCANNNNNTGC 1 cut(s) 2579
BstC8I GCNNGC 8 cut(s) 43, 1242, 1767, 1771, 2099, 2126, 2157, 2521
BstDEI CTNAG 9 cut(s) 87, 231, 498, 550, 606, 800, 1410, 1490, 1629
BstDSI CCRYGG 2 cut(s) 123, 2413
BstENI CCTNNNNNAGG 1 cut(s) 2513
BstF5I GGATG 6 cut(s) 596, 721, 856, 1618, 2999, 3079
BstFNI CGCG 2 cut(s) 68, 185
BstH2I RGCGCY 2 cut(s) 48, 163
BstHHI GCGC 4 cut(s) 47, 70, 136, 162
BstKTI GATC 9 cut(s) 526, 1027, 1129, 1369, 1817, 2234, 2393, 2743, 2989
BstMAI GTCTC 7 cut(s) 57, 88, 1165, 1295, 1406, 1881, 3381
BstMBI GATC 9 cut(s) 523, 1024, 1126, 1366, 1814, 2231, 2390, 2740, 2986
BstMWI GCNNNNNNNGC 7 cut(s) 119, 128, 236, 245, 632, 2195, 2579
BstNSI RCATGY 7 cut(s) 487, 1489, 1546, 1894, 2291, 2849, 3273
BstSFI CTRYAG 4 cut(s) 163, 330, 2190, 2971
BstSLI GKGCMC 1 cut(s) 520
BstUI CGCG 2 cut(s) 68, 185
BstV1I GCAGC 3 cut(s) 118, 2182, 2585
BstV2I GAAGAC 2 cut(s) 356, 3455
BstX2I RGATCY 3 cut(s) 1366, 1814, 2740
BstXI CCANNNNNNTGG 2 cut(s) 1071, 2359
BstYI RGATCY 3 cut(s) 1366, 1814, 2740
BsuI GTATCC 3 cut(s) 2678, 2869, 3283
BtgI CCRYGG 2 cut(s) 123, 2413
BtrI CACGTC 2 cut(s) 510, 2791
BtsCI GGATG 6 cut(s) 596, 721, 856, 1618, 2999, 3079
BtsI GCAGTG 4 cut(s) 243, 642, 1358, 1620
BveI ACCTGC 5 cut(s) 362, 1941, 2170, 3014, 3246
Cac8I GCNNGC 8 cut(s) 43, 1242, 1767, 1771, 2099, 2126, 2157, 2521
CaiI CAGNNNCTG 1 cut(s) 2822
CciI TCATGA 2 cut(s) 1822, 2206
CfoI GCGC 4 cut(s) 47, 70, 136, 162
Cfr13I GGNCC 5 cut(s) 120, 168, 836, 1868, 2378
CseI GACGC 2 cut(s) 74, 2631
CsiI ACCWGGT 1 cut(s) 2380
Csp6I GTAC 6 cut(s) 109, 708, 783, 874, 1321, 3097
CviQI GTAC 6 cut(s) 109, 708, 783, 874, 1321, 3097
DdeI CTNAG 9 cut(s) 87, 231, 498, 550, 606, 800, 1410, 1490, 1629
DpnI GATC 9 cut(s) 525, 1026, 1128, 1368, 1816, 2233, 2392, 2742, 2988
DpnII GATC 9 cut(s) 523, 1024, 1126, 1366, 1814, 2231, 2390, 2740, 2986
EaeI YGGCCR 1 cut(s) 180
Eam1104I CTCTTC 5 cut(s) 143, 429, 590, 2686, 3424
EarI CTCTTC 5 cut(s) 143, 429, 590, 2686, 3424
Ecl136II GAGCTC 2 cut(s) 100, 3215
Eco130I CCWWGG 3 cut(s) 48, 1587, 2686
Eco147I AGGCCT 3 cut(s) 277, 1339, 3466
Eco24I GRGCYC 3 cut(s) 102, 1078, 3217
Eco31I GGTCTC 1 cut(s) 88
Eco32I GATATC 1 cut(s) 3277
Eco47I GGWCC 1 cut(s) 2378
Eco53kI GAGCTC 2 cut(s) 100, 3215
Eco57I CTGAAG 2 cut(s) 2844, 3371
Eco88I CYCGRG 2 cut(s) 74, 3210
EcoICRI GAGCTC 2 cut(s) 100, 3215
EcoNI CCTNNNNNAGG 1 cut(s) 2513
EcoRI GAATTC 2 cut(s) 382, 2681
EcoRV GATATC 1 cut(s) 3277
EcoT14I CCWWGG 3 cut(s) 48, 1587, 2686
EcoT22I ATGCAT 2 cut(s) 2026, 2968
EcoT38I GRGCYC 3 cut(s) 102, 1078, 3217
ErhI CCWWGG 3 cut(s) 48, 1587, 2686
Esp3I CGTCTC 2 cut(s) 57, 1295
FalI AAGNNNNNCTT 6 cut(s) 341, 373, 389, 421, 1307, 1339
FaqI GGGAC 2 cut(s) 496, 676
FauNDI CATATG 1 cut(s) 1165
Fnu4HI GCNGC 6 cut(s) 132, 183, 633, 2196, 2574, 3317
FokI GGATG 6 cut(s) 603, 728, 863, 1625, 2986, 3086
FriOI GRGCYC 3 cut(s) 102, 1078, 3217
Fsp4HI GCNGC 6 cut(s) 132, 183, 633, 2196, 2574, 3317
FspBI CTAG 6 cut(s) 815, 1097, 1460, 1812, 2166, 2657
GlaI GCGC 4 cut(s) 46, 69, 135, 161
GluI GCNGC 6 cut(s) 132, 183, 633, 2196, 2574, 3317
GsaI CCCAGC 1 cut(s) 1351
GsuI CTGGAG 4 cut(s) 469, 1152, 2009, 2335
HaeII RGCGCY 2 cut(s) 48, 163
HapII CCGG 1 cut(s) 2513
HgaI GACGC 2 cut(s) 74, 2631
HhaI GCGC 4 cut(s) 47, 70, 136, 162
Hin6I GCGC 4 cut(s) 45, 68, 134, 160
HinP1I GCGC 4 cut(s) 45, 68, 134, 160
HincII GTYRAC 3 cut(s) 1180, 1222, 1802
HindII GTYRAC 3 cut(s) 1180, 1222, 1802
HindIII AAGCTT 3 cut(s) 2933, 3353, 3457
HpaI GTTAAC 1 cut(s) 1180
HpaII CCGG 1 cut(s) 2513
Hpy166II GTNNAC 6 cut(s) 518, 1180, 1222, 1802, 2387, 3099
Hpy8I GTNNAC 6 cut(s) 518, 1180, 1222, 1802, 2387, 3099
Hpy99I CGWCG 1 cut(s) 120
HpyAV CCTTC 5 cut(s) 73, 250, 926, 1045, 2519
HpyCH4III ACNGT 9 cut(s) 127, 225, 1105, 1226, 1285, 1519, 1738, 2818, 2884
HpyCH4IV ACGT 6 cut(s) 509, 1438, 2370, 2790, 3175, 3370
HpyF10VI GCNNNNNNNGC 7 cut(s) 119, 128, 236, 245, 632, 2195, 2579
HpyF3I CTNAG 9 cut(s) 87, 231, 498, 550, 606, 800, 1410, 1490, 1629
HpySE526I ACGT 6 cut(s) 509, 1438, 2370, 2790, 3175, 3370
HspAI GCGC 4 cut(s) 45, 68, 134, 160
Kpn2I TCCGGA 1 cut(s) 2512
KspAI GTTAAC 1 cut(s) 1180
Kzo9I GATC 9 cut(s) 523, 1024, 1126, 1366, 1814, 2231, 2390, 2740, 2986
LguI GCTCTTC 2 cut(s) 143, 590
LmnI GCTCC 8 cut(s) 38, 105, 724, 1630, 1990, 2587, 3120, 3220
Lsp1109I GCAGC 3 cut(s) 118, 2182, 2585
LweI GCATC 6 cut(s) 619, 706, 1366, 2018, 2084, 2230
MabI ACCWGGT 1 cut(s) 2380
MaeI CTAG 6 cut(s) 815, 1097, 1460, 1812, 2166, 2657
MaeII ACGT 6 cut(s) 509, 1438, 2370, 2790, 3175, 3370
MalI GATC 9 cut(s) 525, 1026, 1128, 1368, 1816, 2233, 2392, 2742, 2988
MbiI CCGCTC 1 cut(s) 3316
MboI GATC 9 cut(s) 523, 1024, 1126, 1366, 1814, 2231, 2390, 2740, 2986
MfeI CAATTG 2 cut(s) 2184, 2919
MflI RGATCY 3 cut(s) 1366, 1814, 2740
MhlI GDGCHC 5 cut(s) 102, 344, 520, 1078, 3217
MlyI GAGTC 2 cut(s) 1148, 1408
MmeI TCCRAC 3 cut(s) 168, 1431, 3383
Mph1103I ATGCAT 2 cut(s) 2026, 2968
MroI TCCGGA 1 cut(s) 2512
MroXI GAANNNNTTC 1 cut(s) 1498
MslI CAYNNNNRTG 3 cut(s) 2768, 2781, 3068
MspA1I CMGCKG 1 cut(s) 923
MspCI CTTAAG 1 cut(s) 2858
MspI CCGG 1 cut(s) 2513
MunI CAATTG 2 cut(s) 2184, 2919
Mva1269I GAATGC 1 cut(s) 2966
MvnI CGCG 2 cut(s) 68, 185
MwoI GCNNNNNNNGC 7 cut(s) 119, 128, 236, 245, 632, 2195, 2579
NdeI CATATG 1 cut(s) 1165
NdeII GATC 9 cut(s) 523, 1024, 1126, 1366, 1814, 2231, 2390, 2740, 2986
NlaIV GGNNCC 6 cut(s) 1309, 1977, 2129, 2654, 2742, 3122
NmeAIII GCCGAG 1 cut(s) 892
NmuCI GTSAC 8 cut(s) 503, 1105, 1616, 1732, 2366, 2786, 2884, 3196
NsiI ATGCAT 2 cut(s) 2026, 2968
NspI RCATGY 7 cut(s) 487, 1489, 1546, 1894, 2291, 2849, 3273
PaeR7I CTCGAG 2 cut(s) 74, 3210
PagI TCATGA 2 cut(s) 1822, 2206
PaqCI CACCTGC 4 cut(s) 1941, 2170, 3014, 3246
PasI CCCWGGG 1 cut(s) 2455
PceI AGGCCT 3 cut(s) 277, 1339, 3466
PciI ACATGT 2 cut(s) 1890, 2287
PciSI GCTCTTC 2 cut(s) 143, 590
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 100
PctI GAATGC 1 cut(s) 2966
PdmI GAANNNNTTC 1 cut(s) 1498
PflFI GACNNNGTC 2 cut(s) 1157, 1226
PflMI CCANNNNNTGG 1 cut(s) 3224
PfoI TCCNGGA 2 cut(s) 721, 1129
PkrI GCNGC 6 cut(s) 133, 184, 634, 2197, 2575, 3318
PleI GAGTC 2 cut(s) 1148, 1408
PpsI GAGTC 2 cut(s) 1148, 1408
PscI ACATGT 2 cut(s) 1890, 2287
PsiI TTATAA 3 cut(s) 2034, 2273, 2931
Psp124BI GAGCTC 2 cut(s) 102, 3217
Psp1406I AACGTT 2 cut(s) 1438, 3175
PspFI CCCAGC 1 cut(s) 1347
PspN4I GGNNCC 6 cut(s) 1309, 1977, 2129, 2654, 2742, 3122
PspPI GGNCC 5 cut(s) 120, 168, 836, 1868, 2378
PsrI GAACNNNNNNTAC 2 cut(s) 3080, 3112
PstNI CAGNNNCTG 1 cut(s) 2822
PsuI RGATCY 3 cut(s) 1366, 1814, 2740
PsyI GACNNNGTC 2 cut(s) 1157, 1226
PvuII CAGCTG 1 cut(s) 923
RsaI GTAC 6 cut(s) 110, 709, 784, 875, 1322, 3098
RsaNI GTAC 6 cut(s) 109, 708, 783, 874, 1321, 3097
RseI CAYNNNNRTG 3 cut(s) 2768, 2781, 3068
SacI GAGCTC 2 cut(s) 102, 3217
SapI GCTCTTC 2 cut(s) 143, 590
SatI GCNGC 6 cut(s) 132, 183, 633, 2196, 2574, 3317
Sau3AI GATC 9 cut(s) 523, 1024, 1126, 1366, 1814, 2231, 2390, 2740, 2986
Sau96I GGNCC 5 cut(s) 120, 168, 836, 1868, 2378
ScaI AGTACT 1 cut(s) 875
SchI GAGTC 2 cut(s) 1148, 1408
SduI GDGCHC 5 cut(s) 102, 344, 520, 1078, 3217
SexAI ACCWGGT 1 cut(s) 2380
SfaNI GCATC 6 cut(s) 619, 706, 1366, 2018, 2084, 2230
SfcI CTRYAG 4 cut(s) 163, 330, 2190, 2971
Sfr274I CTCGAG 2 cut(s) 74, 3210
SinI GGWCC 1 cut(s) 2378
SlaI CTCGAG 2 cut(s) 74, 3210
SmiMI CAYNNNNRTG 3 cut(s) 2768, 2781, 3068
SmlI CTYRAG 6 cut(s) 74, 1211, 1872, 2858, 3210, 3460
SmoI CTYRAG 6 cut(s) 74, 1211, 1872, 2858, 3210, 3460
SseBI AGGCCT 3 cut(s) 277, 1339, 3466
SsiI CCGC 5 cut(s) 183, 632, 2176, 2646, 3316
SspI AATATT 1 cut(s) 1497
SspMI CTAG 6 cut(s) 815, 1097, 1460, 1812, 2166, 2657
SstI GAGCTC 2 cut(s) 102, 3217
StuI AGGCCT 3 cut(s) 277, 1339, 3466
StyI CCWWGG 3 cut(s) 48, 1587, 2686
TaaI ACNGT 9 cut(s) 127, 225, 1105, 1226, 1285, 1519, 1738, 2818, 2884
TaiI ACGT 6 cut(s) 512, 1441, 2373, 2793, 3178, 3373
TaqI TCGA 4 cut(s) 75, 196, 652, 3211
TaqII GACCGA 1 cut(s) 2637
TatI WGTACW 4 cut(s) 707, 782, 873, 1320
TauI GCSGC 3 cut(s) 185, 635, 3319
TseFI GTSAC 8 cut(s) 503, 1105, 1616, 1732, 2366, 2786, 2884, 3196
TseI GCWGC 3 cut(s) 131, 2195, 2573
Tsp45I GTSAC 8 cut(s) 503, 1105, 1616, 1732, 2366, 2786, 2884, 3196
TspGWI ACGGA 5 cut(s) 92, 748, 1111, 2014, 2052
Tth111I GACNNNGTC 2 cut(s) 1157, 1226
Van91I CCANNNNNTGG 1 cut(s) 3224
Vha464I CTTAAG 1 cut(s) 2858
VneI GTGCAC 1 cut(s) 516
VpaK11BI GGWCC 1 cut(s) 2378
XagI CCTNNNNNAGG 1 cut(s) 2513
XapI RAATTY 7 cut(s) 382, 1786, 2449, 2681, 3029, 3135, 3191
XceI RCATGY 7 cut(s) 487, 1489, 1546, 1894, 2291, 2849, 3273
XhoI CTCGAG 2 cut(s) 74, 3210
XmnI GAANNNNTTC 1 cut(s) 1498
XspI CTAG 6 cut(s) 815, 1097, 1460, 1812, 2166, 2657
ZrmI AGTACT 1 cut(s) 875
Zsp2I ATGCAT 2 cut(s) 2026, 2968
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.