Rorug03G0118300

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0010433
Physical Location & Seq
Forward (+)
9595774 .. 9601442
5669 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0118300.1

Sequence Viewer

Length: 1047 bp
ATGGCCGCCGCTACATCCTCCTTCATTGGAACCCGCATCCCCGACGTGTACTCCAACTCGGGCCGGGTCCAGGCCAGATTCGGATTCGGGACCAAGAAGCGGGCCGCGCCCAAGAAGTCCAAGTCCACTTTCACCGACCGTCCGCTCTGGTACCCTGGAGCCAAGGCCCCGGAGTGGCTCGACGGCAGCTTGGTCGGCGACTACGGCTTCGACCCCTTCGGTTTGGGCAAACCCGCCGAGTATTTGCAGTACGACTACGATGGTTTGGACCAGAACCTCGCCAAGAATTTGGCGGGAGATGTCATTGGTACCCGTACCGAAACCGCTGACGTGTCCTCCACACCGTTCCAGCCCTACTCTGAAGTCTTCGGGCTTCAGAGGTTCCGTGAGTGCGAGCTCATCCACGGCCGCTGGGCCATGCTCGCCACCCTCGGAGCCCTCACCGTCGAATCAGTCACCGGAATTACATGGCAAGATGCCGGAAAGGTGGAGCTGATTGAAGGGTCGTCTTACTTGGGACAGCCCCTTCCATTCTCACTGACCACATTGATCTGGATCGAGGTTCTGGTGATCGGGTACATTGAGTTCCAGAGGAACGCTGAGCTCGACCCGGAGAAGAGGTTGTACCCGGGAGGCAAGTTCTTCGACCCATTGGGCTTGGCTGCTGACCCGGAGAAGAAGGCTACACTTCAACTGGCGGAAATCAAGCATGCACGTCTCGCAATGGTTGGGTTCCTTGGATTTGCTGTGCAGGCTGCTGTCACCGATGCTTCTATTCTGCTTCGGCATTTGAGTATCTGCGGAAACCTGGACATGGGTACTCGGAAGGTTTTGTGGGGTTTCGCCAATAAGTTCTTTACGGTTGGGGTAGTGGGTCTCTGTATTTCAGATCGTTTTGTGAGTGTTGCTCCGGTGCGGGGTTCCTCTATGGCTCCTACATTGAATCCTGGAAAGACCAAGTTATTGGGGATGTCAACTGGTTGGTATCTCAAGAGACGTCGTAAACAAATTGCTTTTATGTGCAATGTGATGGTTTGTTTAGCATGA

Protein Analysis

348

Amino Acids

38.13

Weight (kDa)

9.07

Isoelectric Point (pI)

40.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chloroa_b-bind PF00504 56 - 252 9.6e-43 Chlorophyll A-B binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1000
Acc65I GGTACC 2 cut(s) 150, 308
AccB1I GGYRCC 2 cut(s) 150, 308
AccBSI CCGCTC 1 cut(s) 145
AccII CGCG 1 cut(s) 107
AclWI GGATC 1 cut(s) 563
AcoI YGGCCR 2 cut(s) 3, 406
AcsI RAATTY 1 cut(s) 286
AcuI CTGAAG 2 cut(s) 359, 381
AcyI GRCGYC 1 cut(s) 997
AfaI GTAC 8 cut(s) 50, 152, 251, 310, 316, 578, 626, 820
AfiI CCNNNNNNNGG 5 cut(s) 70, 99, 174, 814, 917
AflIII ACRYGT 2 cut(s) 45, 330
AgsI TTSAA 3 cut(s) 500, 692, 943
AjiI CACGTC 3 cut(s) 46, 331, 716
AjnI CCWGG 4 cut(s) 69, 154, 807, 946
AjuI GAANNNNNNNTTGG 2 cut(s) 113, 145
AluBI AGCT 4 cut(s) 189, 397, 493, 604
AluI AGCT 4 cut(s) 189, 397, 493, 604
Alw21I GWGCWC 2 cut(s) 399, 606
Alw26I GTCTC 3 cut(s) 722, 881, 988
AlwI GGATC 1 cut(s) 563
Ama87I CYCGRG 2 cut(s) 58, 628
AoxI GGCC 7 cut(s) 3, 61, 72, 102, 165, 406, 414
ApeKI GCWGC 3 cut(s) 186, 662, 755
ApoI RAATTY 1 cut(s) 286
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
Asp718I GGTACC 2 cut(s) 150, 308
AspLEI GCGC 1 cut(s) 109
AspS9I GGNCC 7 cut(s) 61, 67, 90, 102, 166, 268, 414
AsuC2I CCSGG 6 cut(s) 65, 170, 611, 629, 630, 671
AsuHPI GGTGA 5 cut(s) 124, 433, 448, 580, 754
AvaI CYCGRG 2 cut(s) 58, 628
AvaII GGWCC 3 cut(s) 67, 90, 268
BanI GGYRCC 2 cut(s) 150, 308
BanII GRGCYC 3 cut(s) 399, 439, 606
BarI GAAGNNNNNNTAC 2 cut(s) 608, 640
BbsI GAAGAC 1 cut(s) 358
Bbv12I GWGCWC 2 cut(s) 399, 606
BbvI GCAGC 3 cut(s) 198, 649, 742
BccI CCATC 2 cut(s) 254, 1024
BceAI ACGGC 3 cut(s) 199, 220, 421
BcgI CGANNNNNNTGC 4 cut(s) 175, 209, 625, 659
BciT130I CCWGG 4 cut(s) 71, 156, 809, 948
BcnI CCSGG 6 cut(s) 65, 170, 611, 629, 630, 671
BcoDI GTCTC 3 cut(s) 722, 881, 988
BisI GCNGC 7 cut(s) 6, 9, 105, 187, 409, 663, 756
BlpI GCTNAGC 1 cut(s) 600
BlsI GCNGC 7 cut(s) 7, 10, 106, 188, 410, 664, 757
Bme18I GGWCC 3 cut(s) 67, 90, 268
BmeT110I CYCGRG 2 cut(s) 58, 628
BmgBI CACGTC 3 cut(s) 46, 331, 716
BmgT120I GGNCC 7 cut(s) 61, 67, 90, 102, 166, 268, 414
BmsI GCATC 3 cut(s) 45, 466, 757
BpiI GAAGAC 1 cut(s) 358
BplI GAGNNNNNCTC 2 cut(s) 892, 924
BpmI CTGGAG 1 cut(s) 177
Bpu1102I GCTNAGC 1 cut(s) 600
BpuEI CTTGAG 1 cut(s) 974
BpuMI CCSGG 6 cut(s) 65, 170, 611, 629, 630, 671
BsaBI GATNNNNATC 1 cut(s) 554
BsaHI GRCGYC 1 cut(s) 997
BsaI GGTCTC 1 cut(s) 881
BsaJI CCNNGG 7 cut(s) 154, 162, 168, 403, 430, 628, 736
BsaWI WCCGGW 2 cut(s) 458, 910
BsaXI ACNNNNNCTCC 6 cut(s) 35, 65, 320, 350, 605, 635
Bsc4I CCNNNNNNNGG 5 cut(s) 70, 99, 174, 814, 917
Bse1I ACTGG 2 cut(s) 699, 982
Bse3DI GCAATG 2 cut(s) 729, 1030
Bse8I GATNNNNATC 1 cut(s) 554
BseBI CCWGG 4 cut(s) 71, 156, 809, 948
BseDI CCNNGG 7 cut(s) 154, 162, 168, 403, 430, 628, 736
BseGI GGATG 4 cut(s) 14, 36, 399, 975
BseJI GATNNNNATC 1 cut(s) 554
BseLI CCNNNNNNNGG 5 cut(s) 70, 99, 174, 814, 917
BseMI GCAATG 2 cut(s) 729, 1030
BseMII CTCAG 1 cut(s) 591
BseNI ACTGG 2 cut(s) 699, 982
BseX3I CGGCCG 1 cut(s) 406
BseXI GCAGC 3 cut(s) 198, 649, 742
BseYI CCCAGC 1 cut(s) 411
BsgI GTGCAG 1 cut(s) 770
Bsh1236I CGCG 1 cut(s) 107
Bsh1285I CGRYCG 2 cut(s) 139, 409
BshFI GGCC 7 cut(s) 5, 63, 74, 104, 167, 408, 416
BshNI GGYRCC 2 cut(s) 150, 308
BsiEI CGRYCG 2 cut(s) 139, 409
BsiHKAI GWGCWC 2 cut(s) 399, 606
BsiHKCI CYCGRG 2 cut(s) 58, 628
BsiSI CCGG 8 cut(s) 64, 170, 459, 480, 611, 629, 671, 911
BslFI GGGAC 2 cut(s) 103, 531
BslI CCNNNNNNNGG 5 cut(s) 70, 99, 174, 814, 917
BsmAI GTCTC 3 cut(s) 722, 881, 988
BsmBI CGTCTC 2 cut(s) 722, 988
BsmFI GGGAC 2 cut(s) 103, 531
BsnI GGCC 7 cut(s) 5, 63, 74, 104, 167, 408, 416
Bso31I GGTCTC 1 cut(s) 881
BsoBI CYCGRG 2 cut(s) 58, 628
Bsp1286I GDGCHC 3 cut(s) 399, 439, 606
Bsp143I GATC 4 cut(s) 549, 555, 570, 889
Bsp1720I GCTNAGC 1 cut(s) 600
BspANI GGCC 7 cut(s) 5, 63, 74, 104, 167, 408, 416
BspCNI CTCAG 1 cut(s) 592
BspFNI CGCG 1 cut(s) 107
BspPI GGATC 1 cut(s) 563
BspT107I GGYRCC 2 cut(s) 150, 308
BspTNI GGTCTC 1 cut(s) 881
BsrBI CCGCTC 1 cut(s) 145
BsrDI GCAATG 2 cut(s) 729, 1030
BsrI ACTGG 2 cut(s) 699, 982
BssECI CCNNGG 7 cut(s) 154, 162, 168, 403, 430, 628, 736
BssMI GATC 4 cut(s) 549, 555, 570, 889
BssNI GRCGYC 1 cut(s) 997
BssT1I CCWWGG 2 cut(s) 162, 736
Bst2UI CCWGG 4 cut(s) 71, 156, 809, 948
Bst4CI ACNGT 4 cut(s) 140, 345, 445, 862
Bst6I CTCTTC 1 cut(s) 611
BstACI GRCGYC 1 cut(s) 997
BstC8I GCNNGC 5 cut(s) 102, 395, 423, 711, 753
BstDEI CTNAG 1 cut(s) 600
BstDSI CCRYGG 1 cut(s) 403
BstF5I GGATG 4 cut(s) 14, 36, 399, 975
BstFNI CGCG 1 cut(s) 107
BstHHI GCGC 1 cut(s) 109
BstKTI GATC 4 cut(s) 552, 558, 573, 892
BstMAI GTCTC 3 cut(s) 722, 881, 988
BstMBI GATC 4 cut(s) 549, 555, 570, 889
BstMCI CGRYCG 2 cut(s) 139, 409
BstMWI GCNNNNNNNGC 6 cut(s) 106, 195, 204, 422, 719, 752
BstNI CCWGG 4 cut(s) 71, 156, 809, 948
BstNSI RCATGY 1 cut(s) 713
BstUI CGCG 1 cut(s) 107
BstV1I GCAGC 3 cut(s) 198, 649, 742
BstV2I GAAGAC 1 cut(s) 358
BstXI CCANNNNNNTGG 2 cut(s) 289, 964
BstZI CGGCCG 1 cut(s) 406
BsuRI GGCC 7 cut(s) 5, 63, 74, 104, 167, 408, 416
BtgI CCRYGG 1 cut(s) 403
BtrI CACGTC 3 cut(s) 46, 331, 716
BtsCI GGATG 4 cut(s) 14, 36, 399, 975
BtsIMutI CAGTG 1 cut(s) 536
Cac8I GCNNGC 5 cut(s) 102, 395, 423, 711, 753
CfoI GCGC 1 cut(s) 109
Cfr13I GGNCC 7 cut(s) 61, 67, 90, 102, 166, 268, 414
Cfr9I CCCGGG 1 cut(s) 628
Csp6I GTAC 8 cut(s) 49, 151, 250, 309, 315, 577, 625, 819
CviAII CATG 5 cut(s) 418, 468, 710, 814, 1044
CviQI GTAC 8 cut(s) 49, 151, 250, 309, 315, 577, 625, 819
DdeI CTNAG 1 cut(s) 600
DpnI GATC 4 cut(s) 551, 557, 572, 891
DpnII GATC 4 cut(s) 549, 555, 570, 889
EaeI YGGCCR 2 cut(s) 3, 406
EagI CGGCCG 1 cut(s) 406
Eam1104I CTCTTC 1 cut(s) 611
EarI CTCTTC 1 cut(s) 611
EciI GGCGGA 1 cut(s) 713
Ecl136II GAGCTC 2 cut(s) 397, 604
EclXI CGGCCG 1 cut(s) 406
Eco130I CCWWGG 2 cut(s) 162, 736
Eco24I GRGCYC 3 cut(s) 399, 439, 606
Eco31I GGTCTC 1 cut(s) 881
Eco47I GGWCC 3 cut(s) 67, 90, 268
Eco52I CGGCCG 1 cut(s) 406
Eco53kI GAGCTC 2 cut(s) 397, 604
Eco57I CTGAAG 2 cut(s) 359, 381
Eco88I CYCGRG 2 cut(s) 58, 628
EcoICRI GAGCTC 2 cut(s) 397, 604
EcoO109I RGGNCCY 1 cut(s) 166
EcoRII CCWGG 4 cut(s) 69, 154, 807, 946
EcoT14I CCWWGG 2 cut(s) 162, 736
EcoT38I GRGCYC 3 cut(s) 399, 439, 606
ErhI CCWWGG 2 cut(s) 162, 736
Esp3I CGTCTC 2 cut(s) 722, 988
FaeI CATG 5 cut(s) 421, 471, 713, 817, 1047
FaiI YATR 7 cut(s) 419, 469, 711, 815, 929, 1019, 1045
FaqI GGGAC 2 cut(s) 103, 531
FatI CATG 5 cut(s) 417, 467, 709, 813, 1043
FauI CCCGC 5 cut(s) 41, 93, 241, 286, 909
Fnu4HI GCNGC 7 cut(s) 6, 9, 105, 187, 409, 663, 756
FokI GGATG 3 cut(s) 23, 386, 982
FriOI GRGCYC 3 cut(s) 399, 439, 606
Fsp4HI GCNGC 7 cut(s) 6, 9, 105, 187, 409, 663, 756
GlaI GCGC 1 cut(s) 108
GluI GCNGC 7 cut(s) 6, 9, 105, 187, 409, 663, 756
GsaI CCCAGC 1 cut(s) 415
GsuI CTGGAG 1 cut(s) 177
HaeIII GGCC 7 cut(s) 5, 63, 74, 104, 167, 408, 416
HapII CCGG 8 cut(s) 64, 170, 459, 480, 611, 629, 671, 911
HhaI GCGC 1 cut(s) 109
Hin1I GRCGYC 1 cut(s) 997
Hin1II CATG 5 cut(s) 421, 471, 713, 817, 1047
Hin6I GCGC 1 cut(s) 107
HinP1I GCGC 1 cut(s) 107
HincII GTYRAC 1 cut(s) 975
HindII GTYRAC 1 cut(s) 975
HinfI GANTC 4 cut(s) 78, 84, 449, 943
HpaII CCGG 8 cut(s) 64, 170, 459, 480, 611, 629, 671, 911
HphI GGTGA 5 cut(s) 124, 433, 448, 580, 754
Hpy166II GTNNAC 4 cut(s) 49, 126, 975, 1004
Hpy188I TCNGA 6 cut(s) 83, 361, 378, 434, 825, 889
Hpy188III TCNNGA 4 cut(s) 88, 553, 589, 991
Hpy8I GTNNAC 4 cut(s) 49, 126, 975, 1004
Hpy99I CGWCG 4 cut(s) 47, 185, 449, 1002
HpyAV CCTTC 6 cut(s) 31, 226, 494, 536, 673, 820
HpyCH4III ACNGT 4 cut(s) 140, 345, 445, 862
HpyCH4IV ACGT 4 cut(s) 45, 330, 715, 997
HpyCH4V TGCA 4 cut(s) 247, 713, 751, 1023
HpyF10VI GCNNNNNNNGC 6 cut(s) 106, 195, 204, 422, 719, 752
HpyF3I CTNAG 1 cut(s) 600
HpySE526I ACGT 4 cut(s) 45, 330, 715, 997
Hsp92I GRCGYC 1 cut(s) 997
Hsp92II CATG 5 cut(s) 421, 471, 713, 817, 1047
HspAI GCGC 1 cut(s) 107
KpnI GGTACC 2 cut(s) 154, 312
Kzo9I GATC 4 cut(s) 549, 555, 570, 889
LmnI GCTCC 5 cut(s) 158, 434, 490, 913, 937
Lsp1109I GCAGC 3 cut(s) 198, 649, 742
LweI GCATC 3 cut(s) 45, 466, 757
MaeII ACGT 4 cut(s) 45, 330, 715, 997
MaeIII GTNAC 2 cut(s) 454, 760
MalI GATC 4 cut(s) 551, 557, 572, 891
MbiI CCGCTC 1 cut(s) 145
MboI GATC 4 cut(s) 549, 555, 570, 889
MboII GAAGA 4 cut(s) 358, 628, 634, 688
MhlI GDGCHC 3 cut(s) 399, 439, 606
MluCI AATT 3 cut(s) 286, 462, 1008
MmeI TCCRAC 1 cut(s) 78
MspA1I CMGCKG 2 cut(s) 326, 411
MspI CCGG 8 cut(s) 64, 170, 459, 480, 611, 629, 671, 911
MvaI CCWGG 4 cut(s) 71, 156, 809, 948
MvnI CGCG 1 cut(s) 107
MwoI GCNNNNNNNGC 6 cut(s) 106, 195, 204, 422, 719, 752
NciI CCSGG 6 cut(s) 65, 170, 611, 629, 630, 671
NdeII GATC 4 cut(s) 549, 555, 570, 889
NlaIII CATG 5 cut(s) 421, 471, 713, 817, 1047
NmeAIII GCCGAG 1 cut(s) 262
NmuCI GTSAC 2 cut(s) 454, 760
NspI RCATGY 1 cut(s) 713
PaeI GCATGC 1 cut(s) 713
PcsI WCGNNNNNNNCGW 1 cut(s) 603
PfeI GAWTC 4 cut(s) 78, 84, 449, 943
PfoI TCCNGGA 1 cut(s) 946
PkrI GCNGC 7 cut(s) 7, 10, 106, 188, 410, 664, 757
Psp124BI GAGCTC 2 cut(s) 399, 606
Psp6I CCWGG 4 cut(s) 69, 154, 807, 946
PspFI CCCAGC 1 cut(s) 411
PspGI CCWGG 4 cut(s) 69, 154, 807, 946
PspPI GGNCC 7 cut(s) 61, 67, 90, 102, 166, 268, 414
PsrI GAACNNNNNNTAC 2 cut(s) 569, 601
RsaI GTAC 8 cut(s) 50, 152, 251, 310, 316, 578, 626, 820
RsaNI GTAC 8 cut(s) 49, 151, 250, 309, 315, 577, 625, 819
SacI GAGCTC 2 cut(s) 399, 606
SatI GCNGC 7 cut(s) 6, 9, 105, 187, 409, 663, 756
Sau3AI GATC 4 cut(s) 549, 555, 570, 889
Sau96I GGNCC 7 cut(s) 61, 67, 90, 102, 166, 268, 414
SduI GDGCHC 3 cut(s) 399, 439, 606
SfaNI GCATC 3 cut(s) 45, 466, 757
SinI GGWCC 3 cut(s) 67, 90, 268
SmaI CCCGGG 1 cut(s) 630
SmlI CTYRAG 1 cut(s) 989
SmoI CTYRAG 1 cut(s) 989
SphI GCATGC 1 cut(s) 713
Sse9I AATT 3 cut(s) 286, 462, 1008
SstI GAGCTC 2 cut(s) 399, 606
StyI CCWWGG 2 cut(s) 162, 736
TaaI ACNGT 4 cut(s) 140, 345, 445, 862
TaiI ACGT 4 cut(s) 48, 333, 718, 1000
TaqI TCGA 6 cut(s) 180, 210, 447, 558, 606, 645
TasI AATT 3 cut(s) 286, 462, 1008
TatI WGTACW 1 cut(s) 48
TauI GCSGC 4 cut(s) 8, 11, 107, 411
TfiI GAWTC 4 cut(s) 78, 84, 449, 943
TscAI CASTG 1 cut(s) 543
TseFI GTSAC 2 cut(s) 454, 760
TseI GCWGC 3 cut(s) 186, 662, 755
Tsp45I GTSAC 2 cut(s) 454, 760
TspDTI ATGAA 1 cut(s) 13
TspGWI ACGGA 1 cut(s) 374
TspMI CCCGGG 1 cut(s) 628
TspRI CASTG 1 cut(s) 543
VpaK11BI GGWCC 3 cut(s) 67, 90, 268
XapI RAATTY 1 cut(s) 286
XceI RCATGY 1 cut(s) 713
XmaI CCCGGG 1 cut(s) 628
ZraI GACGTC 1 cut(s) 998
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.