AT3G55500

atexpa4, atexp4, athexp alpha 1.6 atexpa4 (arabidopsis thaliana expansin a4)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014941
Physical Location & Seq
Reverse (-)
20574752 .. 20576249
1498 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G55500.1

Sequence Viewer

Length: 783 bp
ATGGCAATCAATCCATTGATTCTCTTAACCATATTCCCACTCTTCCTTCTCTTGAGCTTCACCGACGCCGGAATCCCTAGAGTCTTCTCAGGTGGTTCTTGGCAAACCGCTCACGCCACTTTCTACGGTGGAAACGATGCTTCGGGAACAATGGGTGGCGCGTGTGGATACGGGAATCTGTATAGCCAAGGATACGGAACAAACACGGCGGCGCTGAGCACATCGTTGTTTAACAGTGGTCAAAGCTGCGGCGCATGCTTCGAAATCAAATGTGTCAATGATCCCAAATGGTGTCACCCGGGTAATCCTTCTGTCTTCGTAACCGCAACCAACTTTTGCCCTCCAAACTTAGCCCAGCCTAGCGACAATGGTGGCTGGTGCAACCCGCCACGCTCTCATTTCGACCTCGCCATGCCCGTTTTCCTCAAGATCGCTGAGTATCGTGCCGGCATTGTCCCCATCTCTTACCGCAGGGTGGCATGTAGGAAGAGTGGAGGGATAAGGTTCACGATCAACGGTCACCGTTACTTCAACTTGGTGCTGATTACGAACGTGGCCGGAGCAGGAGACATCGCGAGGACGAGTGTGAAAGGTTCAAAGACTGGTTGGATGAGTTTAACTAGGAACTGGGGACAGAACTGGCAGTCTAATGCTGTTCTCGTTGGTCAGTCACTTTCCTTCCGTGTTACATCCAGCGATCGTAGAACCTCTACTTCATGGAACATCGCTCCTTCGAACTGGCAGTTTGGACAAACCTTTGTCGGAAAGAATTTCAGGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0006949 GO:0008150 GO:0009653 GO:0009987 GO:0016020 GO:0030312 GO:0032502 GO:0044464 GO:0048646 GO:0048856 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

260

Amino Acids

28.16

Weight (kDa)

9.66

Isoelectric Point (pI)

34.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXPB1_D1 PF22514 39 - 162 3.1e-09 EXPB1-like domain 1
DPBB_1 PF03330 69 - 156 1.3e-21 Lytic transglycolase
Expansin_C PF01357 167 - 244 1.5e-28 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014941)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39700 AT3G55500
fragaria_vesca FvH4_7g22700
malus_domestica MD01G1135600.v1.1 MD07G1200300.v1.1
prunus_persica Prupe.2G237000_v2.0.a1
pyrus_communis pycom01g15640 pycom07g18720
rosa_chinensis RchiOBHm_Chr1g0367791
rosa_laevigata RLG00000027263
rosa_multiflora Rmu_ssc0000395.1_g000022
rosa_roxburghii Rroxscaffold_4G00289220
rosa_rugosa Rorug01G0336600
rosa_samantha Rh1BG305700 Rh1CG321300
rosa_wichuraiana Rw1G030600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 110
AccII CGCG 2 cut(s) 161, 575
AciI CCGC 6 cut(s) 108, 209, 249, 324, 386, 469
AclWI GGATC 1 cut(s) 275
AcoI YGGCCR 1 cut(s) 555
AcsI RAATTY 1 cut(s) 769
AcyI GRCGYC 1 cut(s) 66
AfiI CCNNNNNNNGG 1 cut(s) 475
AgsI TTSAA 2 cut(s) 532, 597
AluBI AGCT 2 cut(s) 57, 246
AluI AGCT 2 cut(s) 57, 246
Alw21I GWGCWC 1 cut(s) 221
Alw26I GTCTC 1 cut(s) 561
AlwI GGATC 1 cut(s) 275
Ama87I CYCGRG 1 cut(s) 298
AoxI GGCC 1 cut(s) 555
ApeKI GCWGC 1 cut(s) 246
ApoI RAATTY 1 cut(s) 769
AspLEI GCGC 3 cut(s) 161, 214, 254
AsuC2I CCSGG 2 cut(s) 299, 300
AsuHPI GGTGA 3 cut(s) 52, 287, 512
AsuII TTCGAA 2 cut(s) 261, 734
AvaI CYCGRG 1 cut(s) 298
BbsI GAAGAC 2 cut(s) 76, 307
Bbv12I GWGCWC 1 cut(s) 221
BbvI GCAGC 1 cut(s) 233
BccI CCATC 1 cut(s) 467
BceAI ACGGC 1 cut(s) 222
BciVI GTATCC 2 cut(s) 161, 185
BcnI CCSGG 2 cut(s) 299, 300
BcoDI GTCTC 1 cut(s) 561
BfaI CTAG 3 cut(s) 78, 360, 621
BfoI RGCGCY 1 cut(s) 215
BfuI GTATCC 2 cut(s) 161, 185
BisI GCNGC 3 cut(s) 210, 247, 250
BlpI GCTNAGC 1 cut(s) 215
BlsI GCNGC 3 cut(s) 211, 248, 251
Bme1390I CCNGG 2 cut(s) 299, 300
BmeT110I CYCGRG 1 cut(s) 298
BmrFI CCNGG 2 cut(s) 299, 300
BmrI ACTGGG 1 cut(s) 637
BmsI GCATC 1 cut(s) 127
BmuI ACTGGG 1 cut(s) 637
BpiI GAAGAC 2 cut(s) 76, 307
Bpu1102I GCTNAGC 1 cut(s) 215
Bpu14I TTCGAA 2 cut(s) 261, 734
BpuEI CTTGAG 2 cut(s) 73, 410
BpuMI CCSGG 2 cut(s) 299, 300
BsaHI GRCGYC 1 cut(s) 66
BsaJI CCNNGG 2 cut(s) 187, 298
Bsc4I CCNNNNNNNGG 1 cut(s) 475
Bse118I RCCGGY 1 cut(s) 446
Bse1I ACTGG 4 cut(s) 607, 632, 644, 743
BseDI CCNNGG 2 cut(s) 187, 298
BseGI GGATG 2 cut(s) 615, 689
BseLI CCNNNNNNNGG 1 cut(s) 475
BseMII CTCAG 3 cut(s) 102, 206, 426
BseNI ACTGG 4 cut(s) 607, 632, 644, 743
BseXI GCAGC 1 cut(s) 233
BseYI CCCAGC 1 cut(s) 354
Bsh1236I CGCG 2 cut(s) 161, 575
Bsh1285I CGRYCG 1 cut(s) 700
BshFI GGCC 1 cut(s) 557
BsiEI CGRYCG 1 cut(s) 700
BsiHKAI GWGCWC 1 cut(s) 221
BsiHKCI CYCGRG 1 cut(s) 298
BsiSI CCGG 4 cut(s) 69, 299, 447, 558
BslFI GGGAC 2 cut(s) 440, 645
BslI CCNNNNNNNGG 1 cut(s) 475
BsmAI GTCTC 1 cut(s) 561
BsmFI GGGAC 2 cut(s) 440, 645
BsnI GGCC 1 cut(s) 557
BsoBI CYCGRG 1 cut(s) 298
Bsp119I TTCGAA 2 cut(s) 261, 734
Bsp1286I GDGCHC 1 cut(s) 221
Bsp143I GATC 4 cut(s) 280, 429, 510, 697
Bsp1720I GCTNAGC 1 cut(s) 215
Bsp68I TCGCGA 1 cut(s) 575
BspACI CCGC 6 cut(s) 108, 209, 249, 324, 386, 469
BspANI GGCC 1 cut(s) 557
BspCNI CTCAG 3 cut(s) 101, 207, 427
BspFNI CGCG 2 cut(s) 161, 575
BspPI GGATC 1 cut(s) 275
BspT104I TTCGAA 2 cut(s) 261, 734
BsrBI CCGCTC 1 cut(s) 110
BsrFI RCCGGY 1 cut(s) 446
BsrI ACTGG 4 cut(s) 607, 632, 644, 743
BssAI RCCGGY 1 cut(s) 446
BssECI CCNNGG 2 cut(s) 187, 298
BssMI GATC 4 cut(s) 280, 429, 510, 697
BssNI GRCGYC 1 cut(s) 66
BssT1I CCWWGG 1 cut(s) 187
Bst4CI ACNGT 4 cut(s) 128, 236, 518, 524
Bst6I CTCTTC 2 cut(s) 47, 482
BstACI GRCGYC 1 cut(s) 66
BstBI TTCGAA 2 cut(s) 261, 734
BstC8I GCNNGC 2 cut(s) 256, 448
BstDEI CTNAG 4 cut(s) 88, 215, 349, 435
BstEII GGTNACC 1 cut(s) 518
BstF5I GGATG 2 cut(s) 615, 689
BstFNI CGCG 2 cut(s) 161, 575
BstH2I RGCGCY 1 cut(s) 215
BstHHI GCGC 3 cut(s) 161, 214, 254
BstKTI GATC 4 cut(s) 283, 432, 513, 700
BstMAI GTCTC 1 cut(s) 561
BstMBI GATC 4 cut(s) 280, 429, 510, 697
BstMCI CGRYCG 1 cut(s) 700
BstMWI GCNNNNNNNGC 1 cut(s) 255
BstNSI RCATGY 2 cut(s) 258, 483
BstPI GGTNACC 1 cut(s) 518
BstSCI CCNGG 2 cut(s) 297, 298
BstUI CGCG 2 cut(s) 161, 575
BstV1I GCAGC 1 cut(s) 233
BstV2I GAAGAC 2 cut(s) 76, 307
BsuI GTATCC 2 cut(s) 161, 185
BsuRI GGCC 1 cut(s) 557
BtgZI GCGATG 2 cut(s) 556, 709
BtsCI GGATG 2 cut(s) 615, 689
BtsIMutI CAGTG 1 cut(s) 241
BtuMI TCGCGA 1 cut(s) 575
Cac8I GCNNGC 2 cut(s) 256, 448
CfoI GCGC 3 cut(s) 161, 214, 254
Cfr10I RCCGGY 1 cut(s) 446
Cfr9I CCCGGG 1 cut(s) 298
CseI GACGC 1 cut(s) 74
CviAII CATG 4 cut(s) 255, 412, 480, 717
CviJI RGCY 7 cut(s) 57, 186, 246, 353, 358, 375, 557
CviKI_1 RGCY 7 cut(s) 57, 186, 246, 353, 358, 375, 557
DdeI CTNAG 4 cut(s) 88, 215, 349, 435
DpnI GATC 4 cut(s) 282, 431, 512, 699
DpnII GATC 4 cut(s) 280, 429, 510, 697
EaeI YGGCCR 1 cut(s) 555
Eam1104I CTCTTC 2 cut(s) 47, 482
EarI CTCTTC 2 cut(s) 47, 482
Eco130I CCWWGG 1 cut(s) 187
Eco88I CYCGRG 1 cut(s) 298
Eco91I GGTNACC 1 cut(s) 518
EcoO65I GGTNACC 1 cut(s) 518
EcoT14I CCWWGG 1 cut(s) 187
ErhI CCWWGG 1 cut(s) 187
FaeI CATG 4 cut(s) 258, 415, 483, 720
FaiI YATR 6 cut(s) 32, 183, 256, 413, 481, 718
FaqI GGGAC 2 cut(s) 440, 645
FatI CATG 4 cut(s) 254, 411, 479, 716
FauI CCCGC 1 cut(s) 393
Fnu4HI GCNGC 3 cut(s) 210, 247, 250
FokI GGATG 2 cut(s) 622, 676
Fsp4HI GCNGC 3 cut(s) 210, 247, 250
FspBI CTAG 3 cut(s) 78, 360, 621
GlaI GCGC 3 cut(s) 160, 213, 253
GluI GCNGC 3 cut(s) 210, 247, 250
GsaI CCCAGC 1 cut(s) 358
HaeII RGCGCY 1 cut(s) 215
HaeIII GGCC 1 cut(s) 557
HapII CCGG 4 cut(s) 69, 299, 447, 558
HgaI GACGC 1 cut(s) 74
HhaI GCGC 3 cut(s) 161, 214, 254
Hin1I GRCGYC 1 cut(s) 66
Hin1II CATG 4 cut(s) 258, 415, 483, 720
Hin6I GCGC 3 cut(s) 159, 212, 252
HinP1I GCGC 3 cut(s) 159, 212, 252
HinfI GANTC 4 cut(s) 19, 72, 81, 175
HpaII CCGG 4 cut(s) 69, 299, 447, 558
HphI GGTGA 3 cut(s) 52, 287, 512
Hpy166II GTNNAC 1 cut(s) 507
Hpy188I TCNGA 1 cut(s) 764
Hpy188III TCNNGA 5 cut(s) 52, 144, 427, 508, 574
Hpy8I GTNNAC 1 cut(s) 507
Hpy99I CGWCG 1 cut(s) 68
HpyAV CCTTC 4 cut(s) 56, 318, 688, 741
HpyCH4III ACNGT 4 cut(s) 128, 236, 518, 524
HpyCH4IV ACGT 1 cut(s) 552
HpyCH4V TGCA 1 cut(s) 381
HpyF10VI GCNNNNNNNGC 1 cut(s) 255
HpyF3I CTNAG 4 cut(s) 88, 215, 349, 435
HpySE526I ACGT 1 cut(s) 552
Hsp92I GRCGYC 1 cut(s) 66
Hsp92II CATG 4 cut(s) 258, 415, 483, 720
HspAI GCGC 3 cut(s) 159, 212, 252
KroI GCCGGC 1 cut(s) 446
KroNI GCCGGC 1 cut(s) 448
Kzo9I GATC 4 cut(s) 280, 429, 510, 697
LmnI GCTCC 2 cut(s) 560, 733
Lsp1109I GCAGC 1 cut(s) 233
LweI GCATC 1 cut(s) 127
MaeI CTAG 3 cut(s) 78, 360, 621
MaeII ACGT 1 cut(s) 552
MaeIII GTNAC 6 cut(s) 293, 319, 518, 524, 669, 685
MalI GATC 4 cut(s) 282, 431, 512, 699
MbiI CCGCTC 1 cut(s) 110
MboI GATC 4 cut(s) 280, 429, 510, 697
MboII GAAGA 4 cut(s) 34, 76, 307, 499
MhlI GDGCHC 1 cut(s) 221
MluCI AATT 1 cut(s) 769
MlyI GAGTC 1 cut(s) 90
MmeI TCCRAC 2 cut(s) 587, 742
MnlI CCTC 6 cut(s) 351, 416, 434, 488, 570, 718
MroNI GCCGGC 1 cut(s) 446
MseI TTAA 3 cut(s) 26, 231, 617
MspI CCGG 4 cut(s) 69, 299, 447, 558
MspR9I CCNGG 2 cut(s) 299, 300
MvnI CGCG 2 cut(s) 161, 575
MwoI GCNNNNNNNGC 1 cut(s) 255
NaeI GCCGGC 1 cut(s) 448
NciI CCSGG 2 cut(s) 299, 300
NdeII GATC 4 cut(s) 280, 429, 510, 697
NgoMIV GCCGGC 1 cut(s) 446
NlaIII CATG 4 cut(s) 258, 415, 483, 720
NmuCI GTSAC 3 cut(s) 293, 518, 669
NruI TCGCGA 1 cut(s) 575
NspI RCATGY 2 cut(s) 258, 483
NspV TTCGAA 2 cut(s) 261, 734
PaeI GCATGC 1 cut(s) 258
PcsI WCGNNNNNNNCGW 2 cut(s) 132, 414
PdiI GCCGGC 1 cut(s) 448
PfeI GAWTC 3 cut(s) 19, 72, 175
PkrI GCNGC 3 cut(s) 211, 248, 251
Ple19I CGATCG 1 cut(s) 700
PleI GAGTC 1 cut(s) 89
PpsI GAGTC 1 cut(s) 89
PspEI GGTNACC 1 cut(s) 518
PspFI CCCAGC 1 cut(s) 354
PvuI CGATCG 1 cut(s) 700
RruI TCGCGA 1 cut(s) 575
SaqAI TTAA 3 cut(s) 26, 231, 617
SatI GCNGC 3 cut(s) 210, 247, 250
Sau3AI GATC 4 cut(s) 280, 429, 510, 697
SchI GAGTC 1 cut(s) 90
ScrFI CCNGG 2 cut(s) 299, 300
SduI GDGCHC 1 cut(s) 221
SetI ASST 9 cut(s) 59, 94, 248, 408, 506, 555, 595, 710, 758
SfaNI GCATC 1 cut(s) 127
SfuI TTCGAA 2 cut(s) 261, 734
SmaI CCCGGG 1 cut(s) 300
SmlI CTYRAG 2 cut(s) 52, 425
SmoI CTYRAG 2 cut(s) 52, 425
SphI GCATGC 1 cut(s) 258
Sse9I AATT 1 cut(s) 769
SsiI CCGC 6 cut(s) 108, 209, 249, 324, 386, 469
SspMI CTAG 3 cut(s) 78, 360, 621
StyD4I CCNGG 2 cut(s) 297, 298
StyI CCWWGG 1 cut(s) 187
TaaI ACNGT 4 cut(s) 128, 236, 518, 524
TaiI ACGT 1 cut(s) 555
TaqI TCGA 3 cut(s) 261, 402, 734
TasI AATT 1 cut(s) 769
TauI GCSGC 2 cut(s) 212, 252
TfiI GAWTC 3 cut(s) 19, 72, 175
Tru1I TTAA 3 cut(s) 26, 231, 617
Tru9I TTAA 3 cut(s) 26, 231, 617
TscAI CASTG 1 cut(s) 241
TseFI GTSAC 3 cut(s) 293, 518, 669
TseI GCWGC 1 cut(s) 246
Tsp45I GTSAC 3 cut(s) 293, 518, 669
TspDTI ATGAA 1 cut(s) 705
TspGWI ACGGA 2 cut(s) 210, 671
TspMI CCCGGG 1 cut(s) 298
TspRI CASTG 1 cut(s) 241
XapI RAATTY 1 cut(s) 769
XceI RCATGY 2 cut(s) 258, 483
XmaI CCCGGG 1 cut(s) 298
XspI CTAG 3 cut(s) 78, 360, 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.