MD01G1135600.v1.1

Pollen allergen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0014941
Physical Location & Seq
Reverse (-)
24472213 .. 24473855
1643 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1135600.v1.1.491

Sequence Viewer

Length: 786 bp
ATGGCCGCCATGGCTCCTCTGCTCTGCATTGCTTCTCTCATGGCACTGGCCAGTACCATTGTAGTCGAGGCCAGAATTCCTGGTGTTTACACTGGCGGAGCGTGGCAGGATGCTCATGCCACCTTCTATGGCGGCAGTGATGCCTCTGGAACTATGGGAGGGGCCTGTGGCTACGGAAATCTGTATAGCCAGGGCTACGGAGTGAACACGGCGGCGCTGAGCACGGCACTCTTCAACAACGGTTTGAGTTGTGGAGCTTGCTTCGAACTCAAGTGCGCAAATCAGCCTCAGTGGTGCAAATCTGGCAGTCCCTCCATTTTTGTCACGGCCACAAACTTTTGCCCTCCAAACTTCGCCCAGCCCAGCGACGATGGCGGCTGGTGCAACCCTCCTCGCACCCACTTCGATCTCGCCATGCCCATGTTCCTCAAGATCGCCGAGTACCGCGCCGGCATTGTCCCTGTTTCTTACCGCCGGGTGCCATGCCGGAAGCAAGGTGGAATCAGGTTCACAATCAACGGCCACAAGTACTTCAACTTGGTTCTGATCCACAACGTCGCGGGCGCAGGGGACATAGTGAAGGTGTACGTTAAAGGGACGAACACTCCGTGGATGAGCCTGAGCCGCAACTGGGGTCAGAACTGGCAGTCAAACGCCATCCTGGTTGGACAGTCACTCTCCTTCAGGGTGACAGCCAGTGACCGCCGCACCTCCACTTCCTGGAACATCGTCCCTTCCAACTGGCAGTTCGGCCAAACTTTCACCGGCAAGAACTTCAGGGTCTAA

Protein Analysis

262

Amino Acids

28.19

Weight (kDa)

9.32

Isoelectric Point (pI)

33.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXPB1_D1 PF22514 40 - 164 7.4e-09 EXPB1-like domain 1
DPBB_1 PF03330 70 - 157 3.6e-22 Lytic transglycolase
Expansin_C PF01357 168 - 245 1.2e-28 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014941)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39700 AT3G55500
fragaria_vesca FvH4_7g22700
malus_domestica MD01G1135600.v1.1 MD07G1200300.v1.1
prunus_persica Prupe.2G237000_v2.0.a1
pyrus_communis pycom01g15640 pycom07g18720
rosa_chinensis RchiOBHm_Chr1g0367791
rosa_laevigata RLG00000027263
rosa_multiflora Rmu_ssc0000395.1_g000022
rosa_roxburghii Rroxscaffold_4G00289220
rosa_rugosa Rorug01G0336600
rosa_samantha Rh1BG305700 Rh1CG321300
rosa_wichuraiana Rw1G030600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 277
AccB1I GGYRCC 1 cut(s) 478
AccB7I CCANNNNNTGG 1 cut(s) 720
AccII CGCG 2 cut(s) 447, 560
AclWI GGATC 1 cut(s) 541
AcoI YGGCCR 5 cut(s) 3, 48, 327, 520, 751
AcsI RAATTY 1 cut(s) 75
AcuI CTGAAG 2 cut(s) 667, 760
AdeI CACNNNGTG 1 cut(s) 609
AfaI GTAC 4 cut(s) 55, 443, 530, 587
AfiI CCNNNNNNNGG 2 cut(s) 631, 720
AgsI TTSAA 2 cut(s) 235, 535
AjnI CCWGG 4 cut(s) 79, 189, 660, 719
AjuI GAANNNNNNNTTGG 2 cut(s) 731, 763
AluBI AGCT 1 cut(s) 257
AluI AGCT 1 cut(s) 257
Alw21I GWGCWC 1 cut(s) 224
AlwI GGATC 1 cut(s) 541
AoxI GGCC 7 cut(s) 3, 48, 69, 162, 327, 520, 751
ApoI RAATTY 1 cut(s) 75
AspLEI GCGC 4 cut(s) 217, 278, 449, 566
AspS9I GGNCC 1 cut(s) 162
AsuC2I CCSGG 1 cut(s) 476
AsuHPI GGTGA 2 cut(s) 700, 754
AsuII TTCGAA 1 cut(s) 264
BalI TGGCCA 1 cut(s) 50
BanI GGYRCC 1 cut(s) 478
Bbv12I GWGCWC 1 cut(s) 224
BccI CCATC 2 cut(s) 365, 665
BceAI ACGGC 4 cut(s) 225, 240, 342, 535
BcgI CGANNNNNNTGC 2 cut(s) 385, 419
BciT130I CCWGG 4 cut(s) 81, 191, 662, 721
BcnI CCSGG 1 cut(s) 476
BfoI RGCGCY 1 cut(s) 218
BglI GCCNNNNNGGC 1 cut(s) 11
BisI GCNGC 6 cut(s) 6, 133, 213, 376, 625, 706
BlpI GCTNAGC 1 cut(s) 218
BlsI GCNGC 6 cut(s) 7, 134, 214, 377, 626, 707
BmcAI AGTACT 1 cut(s) 530
Bme1390I CCNGG 5 cut(s) 81, 191, 476, 662, 721
BmgT120I GGNCC 1 cut(s) 162
BmiI GGNNCC 3 cut(s) 15, 163, 480
BmrFI CCNGG 5 cut(s) 81, 191, 476, 662, 721
BmrI ACTGGG 1 cut(s) 640
BmsI GCATC 2 cut(s) 100, 130
BmuI ACTGGG 1 cut(s) 640
Bpu10I CCTNAGC 1 cut(s) 620
Bpu1102I GCTNAGC 1 cut(s) 218
Bpu14I TTCGAA 1 cut(s) 264
BpuEI CTTGAG 2 cut(s) 254, 413
BpuMI CCSGG 1 cut(s) 476
BsaJI CCNNGG 3 cut(s) 9, 190, 608
BsaXI ACNNNNNCTCC 2 cut(s) 589, 619
Bsc4I CCNNNNNNNGG 2 cut(s) 631, 720
Bse118I RCCGGY 2 cut(s) 449, 764
Bse1I ACTGG 7 cut(s) 51, 51, 97, 635, 647, 696, 746
Bse3DI GCAATG 1 cut(s) 27
BseBI CCWGG 4 cut(s) 81, 191, 662, 721
BseDI CCNNGG 3 cut(s) 9, 190, 608
BseGI GGATG 3 cut(s) 115, 618, 657
BseLI CCNNNNNNNGG 2 cut(s) 631, 720
BseMI GCAATG 1 cut(s) 27
BseMII CTCAG 3 cut(s) 209, 302, 611
BseNI ACTGG 7 cut(s) 51, 51, 97, 635, 647, 696, 746
BseRI GAGGAG 2 cut(s) 6, 381
BseYI CCCAGC 2 cut(s) 357, 362
Bsh1236I CGCG 2 cut(s) 447, 560
BshFI GGCC 7 cut(s) 5, 50, 71, 164, 329, 522, 753
BshNI GGYRCC 1 cut(s) 478
BsiHKAI GWGCWC 1 cut(s) 224
BsiSI CCGG 4 cut(s) 450, 475, 487, 765
BslFI GGGAC 5 cut(s) 294, 443, 584, 610, 716
BslI CCNNNNNNNGG 2 cut(s) 631, 720
BsmFI GGGAC 5 cut(s) 294, 443, 584, 610, 716
BsnI GGCC 7 cut(s) 5, 50, 71, 164, 329, 522, 753
Bsp119I TTCGAA 1 cut(s) 264
Bsp1286I GDGCHC 1 cut(s) 224
Bsp143I GATC 3 cut(s) 406, 432, 546
Bsp1720I GCTNAGC 1 cut(s) 218
Bsp19I CCATGG 1 cut(s) 9
BspANI GGCC 7 cut(s) 5, 50, 71, 164, 329, 522, 753
BspCNI CTCAG 3 cut(s) 210, 301, 612
BspFNI CGCG 2 cut(s) 447, 560
BspLI GGNNCC 3 cut(s) 15, 163, 480
BspPI GGATC 1 cut(s) 541
BspT104I TTCGAA 1 cut(s) 264
BspT107I GGYRCC 1 cut(s) 478
BsrDI GCAATG 1 cut(s) 27
BsrFI RCCGGY 2 cut(s) 449, 764
BsrI ACTGG 7 cut(s) 51, 51, 97, 635, 647, 696, 746
BssAI RCCGGY 2 cut(s) 449, 764
BssECI CCNNGG 3 cut(s) 9, 190, 608
BssMI GATC 3 cut(s) 406, 432, 546
BssT1I CCWWGG 1 cut(s) 9
Bst2UI CCWGG 4 cut(s) 81, 191, 662, 721
Bst4CI ACNGT 2 cut(s) 242, 672
Bst6I CTCTTC 1 cut(s) 236
BstBI TTCGAA 1 cut(s) 264
BstC8I GCNNGC 3 cut(s) 259, 451, 562
BstDEI CTNAG 3 cut(s) 218, 288, 620
BstDSI CCRYGG 2 cut(s) 9, 608
BstF5I GGATG 3 cut(s) 115, 618, 657
BstFNI CGCG 2 cut(s) 447, 560
BstH2I RGCGCY 1 cut(s) 218
BstHHI GCGC 4 cut(s) 217, 278, 449, 566
BstKTI GATC 3 cut(s) 409, 435, 549
BstMBI GATC 3 cut(s) 406, 432, 546
BstMWI GCNNNNNNNGC 5 cut(s) 11, 303, 372, 381, 624
BstNI CCWGG 4 cut(s) 81, 191, 662, 721
BstSCI CCNGG 5 cut(s) 79, 189, 474, 660, 719
BstUI CGCG 2 cut(s) 447, 560
BsuRI GGCC 7 cut(s) 5, 50, 71, 164, 329, 522, 753
BtgI CCRYGG 2 cut(s) 9, 608
BtsCI GGATG 3 cut(s) 115, 618, 657
BtsI GCAGTG 1 cut(s) 142
BtsIMutI CAGTG 5 cut(s) 44, 90, 142, 296, 703
Cac8I GCNNGC 3 cut(s) 259, 451, 562
CfoI GCGC 4 cut(s) 217, 278, 449, 566
Cfr10I RCCGGY 2 cut(s) 449, 764
Cfr13I GGNCC 1 cut(s) 162
Csp6I GTAC 4 cut(s) 54, 442, 529, 586
CviAII CATG 6 cut(s) 10, 40, 116, 415, 421, 483
CviQI GTAC 4 cut(s) 54, 442, 529, 586
DdeI CTNAG 3 cut(s) 218, 288, 620
DpnI GATC 3 cut(s) 408, 434, 548
DpnII GATC 3 cut(s) 406, 432, 546
DraIII CACNNNGTG 1 cut(s) 609
EaeI YGGCCR 5 cut(s) 3, 48, 327, 520, 751
Eam1104I CTCTTC 1 cut(s) 236
EarI CTCTTC 1 cut(s) 236
EciI GGCGGA 1 cut(s) 111
Eco130I CCWWGG 1 cut(s) 9
Eco57I CTGAAG 2 cut(s) 667, 760
EcoO109I RGGNCCY 1 cut(s) 162
EcoRI GAATTC 1 cut(s) 75
EcoRII CCWGG 4 cut(s) 79, 189, 660, 719
EcoT14I CCWWGG 1 cut(s) 9
ErhI CCWWGG 1 cut(s) 9
FaeI CATG 6 cut(s) 13, 43, 119, 418, 424, 486
FaqI GGGAC 5 cut(s) 294, 443, 584, 610, 716
FatI CATG 6 cut(s) 9, 39, 115, 414, 420, 482
FauI CCCGC 1 cut(s) 553
Fnu4HI GCNGC 6 cut(s) 6, 133, 213, 376, 625, 706
FokI GGATG 3 cut(s) 122, 625, 644
Fsp4HI GCNGC 6 cut(s) 6, 133, 213, 376, 625, 706
FspI TGCGCA 1 cut(s) 277
GlaI GCGC 4 cut(s) 216, 277, 448, 565
GluI GCNGC 6 cut(s) 6, 133, 213, 376, 625, 706
GsaI CCCAGC 2 cut(s) 361, 366
HaeII RGCGCY 1 cut(s) 218
HaeIII GGCC 7 cut(s) 5, 50, 71, 164, 329, 522, 753
HapII CCGG 4 cut(s) 450, 475, 487, 765
HhaI GCGC 4 cut(s) 217, 278, 449, 566
Hin1II CATG 6 cut(s) 13, 43, 119, 418, 424, 486
Hin6I GCGC 4 cut(s) 215, 276, 447, 564
HinP1I GCGC 4 cut(s) 215, 276, 447, 564
HinfI GANTC 1 cut(s) 501
HpaII CCGG 4 cut(s) 450, 475, 487, 765
HphI GGTGA 2 cut(s) 700, 754
Hpy166II GTNNAC 4 cut(s) 88, 205, 510, 586
Hpy188I TCNGA 2 cut(s) 546, 639
Hpy188III TCNNGA 2 cut(s) 147, 430
Hpy8I GTNNAC 4 cut(s) 88, 205, 510, 586
Hpy99I CGWCG 2 cut(s) 371, 560
HpyAV CCTTC 4 cut(s) 133, 574, 691, 744
HpyCH4III ACNGT 2 cut(s) 242, 672
HpyCH4IV ACGT 2 cut(s) 555, 588
HpyCH4V TGCA 3 cut(s) 27, 297, 384
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 303, 372, 381, 624
HpyF3I CTNAG 3 cut(s) 218, 288, 620
HpySE526I ACGT 2 cut(s) 555, 588
Hsp92II CATG 6 cut(s) 13, 43, 119, 418, 424, 486
HspAI GCGC 4 cut(s) 215, 276, 447, 564
KroI GCCGGC 1 cut(s) 449
KroNI GCCGGC 1 cut(s) 451
Kzo9I GATC 3 cut(s) 406, 432, 546
LmnI GCTCC 3 cut(s) 19, 98, 254
LweI GCATC 2 cut(s) 100, 130
MaeII ACGT 2 cut(s) 555, 588
MaeIII GTNAC 4 cut(s) 322, 672, 688, 698
MalI GATC 3 cut(s) 408, 434, 548
MboI GATC 3 cut(s) 406, 432, 546
MboII GAAGA 1 cut(s) 223
MhlI GDGCHC 1 cut(s) 224
MlsI TGGCCA 1 cut(s) 50
MluCI AATT 1 cut(s) 75
MluNI TGGCCA 1 cut(s) 50
MmeI TCCRAC 2 cut(s) 646, 762
Mox20I TGGCCA 1 cut(s) 50
MroNI GCCGGC 1 cut(s) 449
MscI TGGCCA 1 cut(s) 50
MseI TTAA 1 cut(s) 591
MslI CAYNNNNRTG 1 cut(s) 419
Msp20I TGGCCA 1 cut(s) 50
MspI CCGG 4 cut(s) 450, 475, 487, 765
MspR9I CCNGG 5 cut(s) 81, 191, 476, 662, 721
MvaI CCWGG 4 cut(s) 81, 191, 662, 721
MvnI CGCG 2 cut(s) 447, 560
MwoI GCNNNNNNNGC 5 cut(s) 11, 303, 372, 381, 624
NaeI GCCGGC 1 cut(s) 451
NciI CCSGG 1 cut(s) 476
NcoI CCATGG 1 cut(s) 9
NdeII GATC 3 cut(s) 406, 432, 546
NgoMIV GCCGGC 1 cut(s) 449
NlaIII CATG 6 cut(s) 13, 43, 119, 418, 424, 486
NlaIV GGNNCC 3 cut(s) 15, 163, 480
NmeAIII GCCGAG 1 cut(s) 463
NmuCI GTSAC 4 cut(s) 322, 672, 688, 698
NsbI TGCGCA 1 cut(s) 277
NspV TTCGAA 1 cut(s) 264
PcsI WCGNNNNNNNCGW 1 cut(s) 605
PdiI GCCGGC 1 cut(s) 451
PfeI GAWTC 1 cut(s) 501
PflMI CCANNNNNTGG 1 cut(s) 720
PfoI TCCNGGA 1 cut(s) 719
PkrI GCNGC 6 cut(s) 7, 134, 214, 377, 626, 707
Psp6I CCWGG 4 cut(s) 79, 189, 660, 719
PspFI CCCAGC 2 cut(s) 357, 362
PspGI CCWGG 4 cut(s) 79, 189, 660, 719
PspN4I GGNNCC 3 cut(s) 15, 163, 480
PspPI GGNCC 1 cut(s) 162
RsaI GTAC 4 cut(s) 55, 443, 530, 587
RsaNI GTAC 4 cut(s) 54, 442, 529, 586
RseI CAYNNNNRTG 1 cut(s) 419
SaqAI TTAA 1 cut(s) 591
SatI GCNGC 6 cut(s) 6, 133, 213, 376, 625, 706
Sau3AI GATC 3 cut(s) 406, 432, 546
Sau96I GGNCC 1 cut(s) 162
ScaI AGTACT 1 cut(s) 530
ScrFI CCNGG 5 cut(s) 81, 191, 476, 662, 721
SduI GDGCHC 1 cut(s) 224
SetI ASST 8 cut(s) 125, 259, 499, 509, 558, 585, 591, 713
SfaNI GCATC 2 cut(s) 100, 130
SfuI TTCGAA 1 cut(s) 264
SmiMI CAYNNNNRTG 1 cut(s) 419
SmlI CTYRAG 2 cut(s) 269, 428
SmoI CTYRAG 2 cut(s) 269, 428
Sse9I AATT 1 cut(s) 75
StyD4I CCNGG 5 cut(s) 79, 189, 474, 660, 719
StyI CCWWGG 1 cut(s) 9
TaaI ACNGT 2 cut(s) 242, 672
TaiI ACGT 2 cut(s) 558, 591
TaqI TCGA 3 cut(s) 66, 264, 405
TasI AATT 1 cut(s) 75
TatI WGTACW 1 cut(s) 528
TauI GCSGC 6 cut(s) 8, 135, 215, 378, 627, 708
TfiI GAWTC 1 cut(s) 501
Tru1I TTAA 1 cut(s) 591
Tru9I TTAA 1 cut(s) 591
TscAI CASTG 5 cut(s) 51, 97, 142, 296, 703
TseFI GTSAC 4 cut(s) 322, 672, 688, 698
Tsp45I GTSAC 4 cut(s) 322, 672, 688, 698
TspGWI ACGGA 3 cut(s) 189, 213, 597
TspRI CASTG 5 cut(s) 51, 97, 142, 296, 703
Van91I CCANNNNNTGG 1 cut(s) 720
XapI RAATTY 1 cut(s) 75
ZrmI AGTACT 1 cut(s) 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.