Rh1CG321300

Pollen allergen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0014941
Physical Location & Seq
Reverse (-)
59616512 .. 59618081
1570 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG321300.1

Sequence Viewer

Length: 777 bp
ATGGCTTTCATGTCTCTGCTCTGCATTGTTTCCCTCATTGCGTTCTCAACAGTTTTCGTTGACGCAAATGTTCCCGGTGTTTACTCCGGCGGACAATGGAAGAAGGCCCATGCTACTTTCTATGGTGGCGGTGACGCCGCCGGAACCATGCAAGGGGCATGTGGGTACGGAAATCTGTACAGCGACGGCTACGGCATCAACACGGCGGCGCTGAGCACTGCCCTCTTCAACAACGGTTTGACCTGTGGAGCTTGTTTTGAAATTAAGTGTGTAAACACCAAATGGTGTCACTCTGGTAGTCCCTCGATCTTTGTCACCGCCACCAATTTTTGCCCCCCAAATTGGGCTCAGGCCAGTGACAATGGCGGCTGGTGCAATCCTCCTCGCACTCATTTCGATCTCTCTCAGCCCATCTTTCTCAAGATCGCCGAGTACCGCGCCGGCATTGTCCCTGTTGTATATCGCCGGGTGCCATGCCGGAAGCGAGGAGGGATCAGGTTCAAGATCAATGGCAACCCCCACTTCAACTTGGTTCTGATCACCAACGTCGCCGGTGCTGGGGACGTTGTGGGGGTGAGCATCAAAGGGTCCAAAACTGGATGGATGCCAATGACGCGTAACTGGGGGCAGAACTGGCAGTCAGGCACCGTCTTGGTCGGACAGTCGCTCTCCTTCAGTGTCAAAACCAGTGACGGTCGCCACACTACTTCTTGGAACGTTGTCCCGTCCAATTGGCAGTTTGGTCAATCTTTCACCGGAAAAAACTTCAGAGTCTGA

Protein Analysis

258

Amino Acids

27.82

Weight (kDa)

9.48

Isoelectric Point (pI)

22.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 68 - 154 3.2e-21 Lytic transglycolase
Expansin_C PF01357 165 - 242 4.8e-28 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014941)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39700 AT3G55500
fragaria_vesca FvH4_7g22700
malus_domestica MD01G1135600.v1.1 MD07G1200300.v1.1
prunus_persica Prupe.2G237000_v2.0.a1
pyrus_communis pycom01g15640 pycom07g18720
rosa_chinensis RchiOBHm_Chr1g0367791
rosa_laevigata RLG00000027263
rosa_multiflora Rmu_ssc0000395.1_g000022
rosa_roxburghii Rroxscaffold_4G00289220
rosa_rugosa Rorug01G0336600
rosa_samantha Rh1BG305700 Rh1CG321300
rosa_wichuraiana Rw1G030600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 469, 644
AccII CGCG 2 cut(s) 438, 616
AciI CCGC 7 cut(s) 90, 129, 138, 206, 318, 366, 436
AclI AACGTT 1 cut(s) 717
AclWI GGATC 1 cut(s) 500
AcuI CTGAAG 2 cut(s) 658, 751
AcyI GRCGYC 1 cut(s) 135
AfaI GTAC 3 cut(s) 167, 179, 434
AfiI CCNNNNNNNGG 4 cut(s) 153, 342, 343, 558
AflIII ACRYGT 1 cut(s) 614
AgsI TTSAA 4 cut(s) 229, 260, 502, 526
AluBI AGCT 1 cut(s) 251
AluI AGCT 1 cut(s) 251
Alw21I GWGCWC 1 cut(s) 218
Alw26I GTCTC 1 cut(s) 18
AlwI GGATC 1 cut(s) 500
AlwNI CAGNNNCTG 1 cut(s) 774
AoxI GGCC 2 cut(s) 105, 351
AspLEI GCGC 2 cut(s) 211, 440
AspS9I GGNCC 2 cut(s) 106, 588
AsuC2I CCSGG 2 cut(s) 75, 467
AsuHPI GGTGA 5 cut(s) 143, 307, 532, 586, 745
AvaII GGWCC 1 cut(s) 588
BanI GGYRCC 2 cut(s) 469, 644
BanII GRGCYC 1 cut(s) 349
Bbv12I GWGCWC 1 cut(s) 218
BccI CCATC 2 cut(s) 419, 594
BceAI ACGGC 3 cut(s) 202, 208, 219
BcgI CGANNNNNNTGC 2 cut(s) 376, 410
BclI TGATCA 1 cut(s) 537
BcnI CCSGG 2 cut(s) 75, 467
BcoDI GTCTC 1 cut(s) 18
BfoI RGCGCY 1 cut(s) 212
BisI GCNGC 3 cut(s) 138, 207, 367
BlpI GCTNAGC 1 cut(s) 212
BlsI GCNGC 3 cut(s) 139, 208, 368
Bme1390I CCNGG 2 cut(s) 75, 467
Bme18I GGWCC 1 cut(s) 588
BmgT120I GGNCC 2 cut(s) 106, 588
BmiI GGNNCC 4 cut(s) 145, 471, 589, 646
BmrFI CCNGG 2 cut(s) 75, 467
BmrI ACTGGG 1 cut(s) 631
BmsI GCATC 3 cut(s) 204, 588, 594
BmuI ACTGGG 1 cut(s) 631
Bpu10I CCTNAGC 1 cut(s) 348
Bpu1102I GCTNAGC 1 cut(s) 212
BpuEI CTTGAG 1 cut(s) 404
BpuMI CCSGG 2 cut(s) 75, 467
BsaHI GRCGYC 1 cut(s) 135
BsaWI WCCGGW 1 cut(s) 755
Bsc4I CCNNNNNNNGG 4 cut(s) 153, 342, 343, 558
Bse118I RCCGGY 2 cut(s) 440, 551
Bse1I ACTGG 5 cut(s) 354, 601, 626, 638, 687
Bse3DI GCAATG 1 cut(s) 36
BseGI GGATG 2 cut(s) 605, 609
BseLI CCNNNNNNNGG 4 cut(s) 153, 342, 343, 558
BseMI GCAATG 1 cut(s) 36
BseMII CTCAG 3 cut(s) 203, 362, 419
BseNI ACTGG 5 cut(s) 354, 601, 626, 638, 687
BseRI GAGGAG 2 cut(s) 372, 501
BseYI CCCAGC 1 cut(s) 557
Bsh1236I CGCG 2 cut(s) 438, 616
Bsh1285I CGRYCG 1 cut(s) 697
BshFI GGCC 2 cut(s) 107, 353
BshNI GGYRCC 2 cut(s) 469, 644
BsiEI CGRYCG 1 cut(s) 697
BsiHKAI GWGCWC 1 cut(s) 218
BsiSI CCGG 8 cut(s) 75, 87, 141, 441, 466, 478, 552, 756
BslFI GGGAC 4 cut(s) 285, 434, 575, 707
BslI CCNNNNNNNGG 4 cut(s) 153, 342, 343, 558
BsmAI GTCTC 1 cut(s) 18
BsmFI GGGAC 4 cut(s) 285, 434, 575, 707
BsnI GGCC 2 cut(s) 107, 353
Bsp1286I GDGCHC 2 cut(s) 218, 349
Bsp1407I TGTACA 1 cut(s) 177
Bsp143I GATC 6 cut(s) 306, 397, 423, 492, 504, 537
Bsp1720I GCTNAGC 1 cut(s) 212
BspACI CCGC 7 cut(s) 90, 129, 138, 206, 318, 366, 436
BspANI GGCC 2 cut(s) 107, 353
BspCNI CTCAG 3 cut(s) 204, 361, 418
BspFNI CGCG 2 cut(s) 438, 616
BspLI GGNNCC 4 cut(s) 145, 471, 589, 646
BspPI GGATC 1 cut(s) 500
BspT107I GGYRCC 2 cut(s) 469, 644
BsrDI GCAATG 1 cut(s) 36
BsrFI RCCGGY 2 cut(s) 440, 551
BsrGI TGTACA 1 cut(s) 177
BsrI ACTGG 5 cut(s) 354, 601, 626, 638, 687
BssAI RCCGGY 2 cut(s) 440, 551
BssMI GATC 6 cut(s) 306, 397, 423, 492, 504, 537
BssNI GRCGYC 1 cut(s) 135
Bst4CI ACNGT 5 cut(s) 52, 236, 649, 663, 695
Bst6I CTCTTC 1 cut(s) 230
BstACI GRCGYC 1 cut(s) 135
BstAUI TGTACA 1 cut(s) 177
BstC8I GCNNGC 1 cut(s) 442
BstDEI CTNAG 3 cut(s) 212, 348, 405
BstF5I GGATG 2 cut(s) 605, 609
BstFNI CGCG 2 cut(s) 438, 616
BstH2I RGCGCY 1 cut(s) 212
BstHHI GCGC 2 cut(s) 211, 440
BstKTI GATC 6 cut(s) 309, 400, 426, 495, 507, 540
BstMAI GTCTC 1 cut(s) 18
BstMBI GATC 6 cut(s) 306, 397, 423, 492, 504, 537
BstMCI CGRYCG 1 cut(s) 697
BstMWI GCNNNNNNNGC 3 cut(s) 372, 613, 634
BstNSI RCATGY 1 cut(s) 162
BstSCI CCNGG 2 cut(s) 73, 465
BstUI CGCG 2 cut(s) 438, 616
BsuRI GGCC 2 cut(s) 107, 353
BtsCI GGATG 2 cut(s) 605, 609
BtsI GCAGTG 1 cut(s) 216
BtsIMutI CAGTG 4 cut(s) 216, 361, 682, 694
Cac8I GCNNGC 1 cut(s) 442
CaiI CAGNNNCTG 1 cut(s) 774
CfoI GCGC 2 cut(s) 211, 440
Cfr10I RCCGGY 2 cut(s) 440, 551
Cfr13I GGNCC 2 cut(s) 106, 588
CseI GACGC 3 cut(s) 71, 143, 622
Csp6I GTAC 3 cut(s) 166, 178, 433
CviAII CATG 5 cut(s) 10, 110, 148, 159, 474
CviJI RGCY 8 cut(s) 5, 107, 189, 251, 347, 353, 369, 409
CviKI_1 RGCY 8 cut(s) 5, 107, 189, 251, 347, 353, 369, 409
CviQI GTAC 3 cut(s) 166, 178, 433
DdeI CTNAG 3 cut(s) 212, 348, 405
DpnI GATC 6 cut(s) 308, 399, 425, 494, 506, 539
DpnII GATC 6 cut(s) 306, 397, 423, 492, 504, 537
Eam1104I CTCTTC 1 cut(s) 230
EarI CTCTTC 1 cut(s) 230
EciI GGCGGA 1 cut(s) 105
Eco24I GRGCYC 1 cut(s) 349
Eco47I GGWCC 1 cut(s) 588
Eco57I CTGAAG 2 cut(s) 658, 751
EcoT38I GRGCYC 1 cut(s) 349
FaeI CATG 5 cut(s) 13, 113, 151, 162, 477
FaiI YATR 7 cut(s) 11, 111, 123, 149, 160, 460, 475
FaqI GGGAC 4 cut(s) 285, 434, 575, 707
FatI CATG 5 cut(s) 9, 109, 147, 158, 473
FbaI TGATCA 1 cut(s) 537
Fnu4HI GCNGC 3 cut(s) 138, 207, 367
FokI GGATG 2 cut(s) 612, 616
FriOI GRGCYC 1 cut(s) 349
Fsp4HI GCNGC 3 cut(s) 138, 207, 367
GlaI GCGC 2 cut(s) 210, 439
GluI GCNGC 3 cut(s) 138, 207, 367
GsaI CCCAGC 1 cut(s) 561
HaeII RGCGCY 1 cut(s) 212
HaeIII GGCC 2 cut(s) 107, 353
HapII CCGG 8 cut(s) 75, 87, 141, 441, 466, 478, 552, 756
HgaI GACGC 3 cut(s) 71, 143, 622
HhaI GCGC 2 cut(s) 211, 440
Hin1I GRCGYC 1 cut(s) 135
Hin1II CATG 5 cut(s) 13, 113, 151, 162, 477
Hin6I GCGC 2 cut(s) 209, 438
HinP1I GCGC 2 cut(s) 209, 438
HincII GTYRAC 1 cut(s) 61
HindII GTYRAC 1 cut(s) 61
HinfI GANTC 1 cut(s) 771
HpaII CCGG 8 cut(s) 75, 87, 141, 441, 466, 478, 552, 756
HphI GGTGA 5 cut(s) 143, 307, 532, 586, 745
Hpy166II GTNNAC 3 cut(s) 61, 82, 274
Hpy188I TCNGA 4 cut(s) 537, 659, 770, 776
Hpy188III TCNNGA 2 cut(s) 421, 502
Hpy8I GTNNAC 3 cut(s) 61, 82, 274
Hpy99I CGWCG 2 cut(s) 188, 551
HpyAV CCTTC 2 cut(s) 97, 682
HpyCH4III ACNGT 5 cut(s) 52, 236, 649, 663, 695
HpyCH4IV ACGT 3 cut(s) 546, 564, 717
HpyCH4V TGCA 3 cut(s) 24, 151, 375
HpyF10VI GCNNNNNNNGC 3 cut(s) 372, 613, 634
HpyF3I CTNAG 3 cut(s) 212, 348, 405
HpySE526I ACGT 3 cut(s) 546, 564, 717
Hsp92I GRCGYC 1 cut(s) 135
Hsp92II CATG 5 cut(s) 13, 113, 151, 162, 477
HspAI GCGC 2 cut(s) 209, 438
KroI GCCGGC 1 cut(s) 440
KroNI GCCGGC 1 cut(s) 442
Ksp22I TGATCA 1 cut(s) 537
Kzo9I GATC 6 cut(s) 306, 397, 423, 492, 504, 537
LmnI GCTCC 1 cut(s) 248
LweI GCATC 3 cut(s) 204, 588, 594
MaeII ACGT 3 cut(s) 546, 564, 717
MaeIII GTNAC 6 cut(s) 131, 287, 313, 356, 617, 689
MalI GATC 6 cut(s) 308, 399, 425, 494, 506, 539
MboI GATC 6 cut(s) 306, 397, 423, 492, 504, 537
MboII GAAGA 2 cut(s) 112, 217
MfeI CAATTG 1 cut(s) 730
MhlI GDGCHC 2 cut(s) 218, 349
MluCI AATT 4 cut(s) 261, 325, 340, 730
MluI ACGCGT 1 cut(s) 614
MmeI TCCRAC 1 cut(s) 637
MnlI CCTC 7 cut(s) 44, 233, 313, 390, 393, 479, 482
MroNI GCCGGC 1 cut(s) 440
MseI TTAA 1 cut(s) 264
MspI CCGG 8 cut(s) 75, 87, 141, 441, 466, 478, 552, 756
MspR9I CCNGG 2 cut(s) 75, 467
MunI CAATTG 1 cut(s) 730
MvnI CGCG 2 cut(s) 438, 616
MwoI GCNNNNNNNGC 3 cut(s) 372, 613, 634
NaeI GCCGGC 1 cut(s) 442
NciI CCSGG 2 cut(s) 75, 467
NdeII GATC 6 cut(s) 306, 397, 423, 492, 504, 537
NgoMIV GCCGGC 1 cut(s) 440
NlaIII CATG 5 cut(s) 13, 113, 151, 162, 477
NlaIV GGNNCC 4 cut(s) 145, 471, 589, 646
NmeAIII GCCGAG 1 cut(s) 454
NmuCI GTSAC 5 cut(s) 131, 287, 313, 356, 689
NspI RCATGY 1 cut(s) 162
PdiI GCCGGC 1 cut(s) 442
PkrI GCNGC 3 cut(s) 139, 208, 368
Psp1406I AACGTT 1 cut(s) 717
PspFI CCCAGC 1 cut(s) 557
PspN4I GGNNCC 4 cut(s) 145, 471, 589, 646
PspPI GGNCC 2 cut(s) 106, 588
PstNI CAGNNNCTG 1 cut(s) 774
RsaI GTAC 3 cut(s) 167, 179, 434
RsaNI GTAC 3 cut(s) 166, 178, 433
SaqAI TTAA 1 cut(s) 264
SatI GCNGC 3 cut(s) 138, 207, 367
Sau3AI GATC 6 cut(s) 306, 397, 423, 492, 504, 537
Sau96I GGNCC 2 cut(s) 106, 588
ScrFI CCNGG 2 cut(s) 75, 467
SduI GDGCHC 2 cut(s) 218, 349
SetI ASST 6 cut(s) 245, 253, 500, 549, 567, 720
SfaNI GCATC 3 cut(s) 204, 588, 594
SgrAI CRCCGGYG 1 cut(s) 551
SinI GGWCC 1 cut(s) 588
SmlI CTYRAG 1 cut(s) 419
SmoI CTYRAG 1 cut(s) 419
Sse9I AATT 4 cut(s) 261, 325, 340, 730
SsiI CCGC 7 cut(s) 90, 129, 138, 206, 318, 366, 436
StyD4I CCNGG 2 cut(s) 73, 465
TaaI ACNGT 5 cut(s) 52, 236, 649, 663, 695
TaiI ACGT 3 cut(s) 549, 567, 720
TaqI TCGA 2 cut(s) 305, 396
TasI AATT 4 cut(s) 261, 325, 340, 730
TatI WGTACW 1 cut(s) 177
TauI GCSGC 3 cut(s) 140, 209, 369
Tru1I TTAA 1 cut(s) 264
Tru9I TTAA 1 cut(s) 264
TscAI CASTG 4 cut(s) 223, 361, 682, 694
TseFI GTSAC 5 cut(s) 131, 287, 313, 356, 689
Tsp45I GTSAC 5 cut(s) 131, 287, 313, 356, 689
TspGWI ACGGA 1 cut(s) 183
TspRI CASTG 4 cut(s) 223, 361, 682, 694
VpaK11BI GGWCC 1 cut(s) 588
XceI RCATGY 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.