RLG00000027263

Pollen allergen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0014941
Physical Location & Seq
Forward (+)
10137962 .. 10139518
1557 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027263

Sequence Viewer

Length: 777 bp
ATGGCCTTCATGTCTGTGCTCTACATTGTTTCCCTCATTGCATTCTCAACAGTTTTCGGTGACGCAAATGTTCCCGGTGTTTACTCCGGCGGACAATGGAAGAAGGCCCATGCTACTTTCTATGGTGGCGGTGACGCCACCGGAACCATGGGAGGGGCATGTGGGTACGGAAATCTGTACAGCGACGGCTACGGCATCAACACGGCGGCGCTGAGCACTGCCCTCTTCAACAACGGTTTGAGCTGTGGAGCTTGTTTTGAAATTAAGTGTGTAAACACCAAATGGTGTCACTCTGGTAGACCCTCGATCTTTGTCACCGCCACCAATTTTTGCCCCCCAAATTGGGCTCAGGCCAGTGACAATGGCGGCTGGTGCAACCCTCCTCGCACTCATTTCGATCTCTCTCAGCCCATCTTTCTCAAGATCGCCGAGTACCGCGCCGGCATTGTCCCTGTCGCATATCGCCGGGTGCCATGCCGGAAGCGAGGAGGGATCAGGTTCAAGATCAATGGCAACCCCCACTTCAACTTGGTTCTGATCTCCAACGTCGCCGGTGCTGGAGACGTTGTGGGGGTGAGCATCAAGGGGTCCAAAACTGGATGGATGCCAATGAGACGGAACTGGGGGCAGAACTGGCAGTCAGACACCGTCTTGGTCGGACAGTCGCTCTCCTTCAGTGTCAAAACCAGTGACGGTCGCCACACTACTTCTTGGAACGTTGTCCCTTCCAATTGGCAGTTTGGTCAATCTTTCACCGGAAATAACTTCAGAGTCTGA

Protein Analysis

259

Amino Acids

27.89

Weight (kDa)

9.55

Isoelectric Point (pI)

26.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXPB1_D1 PF22514 37 - 160 3.4e-08 EXPB1-like domain 1
DPBB_1 PF03330 68 - 154 9.9e-22 Lytic transglycolase
Expansin_C PF01357 165 - 242 2.6e-28 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014941)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39700 AT3G55500
fragaria_vesca FvH4_7g22700
malus_domestica MD01G1135600.v1.1 MD07G1200300.v1.1
prunus_persica Prupe.2G237000_v2.0.a1
pyrus_communis pycom01g15640 pycom07g18720
rosa_chinensis RchiOBHm_Chr1g0367791
rosa_laevigata RLG00000027263
rosa_multiflora Rmu_ssc0000395.1_g000022
rosa_roxburghii Rroxscaffold_4G00289220
rosa_rugosa Rorug01G0336600
rosa_samantha Rh1BG305700 Rh1CG321300
rosa_wichuraiana Rw1G030600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 469
AccI GTMKAC 1 cut(s) 298
AccII CGCG 1 cut(s) 438
AciI CCGC 6 cut(s) 90, 129, 206, 318, 366, 436
AclI AACGTT 1 cut(s) 717
AclWI GGATC 1 cut(s) 500
AcuI CTGAAG 2 cut(s) 658, 751
AcyI GRCGYC 1 cut(s) 135
AfaI GTAC 3 cut(s) 167, 179, 434
AfiI CCNNNNNNNGG 3 cut(s) 153, 342, 343
AgsI TTSAA 4 cut(s) 229, 260, 502, 526
AluBI AGCT 2 cut(s) 243, 251
AluI AGCT 2 cut(s) 243, 251
Alw21I GWGCWC 2 cut(s) 21, 218
Alw26I GTCTC 2 cut(s) 555, 607
AlwI GGATC 1 cut(s) 500
AlwNI CAGNNNCTG 1 cut(s) 774
AoxI GGCC 3 cut(s) 3, 105, 351
AspLEI GCGC 2 cut(s) 211, 440
AspS9I GGNCC 2 cut(s) 106, 588
AsuC2I CCSGG 2 cut(s) 75, 467
AsuHPI GGTGA 5 cut(s) 71, 143, 307, 586, 745
AvaII GGWCC 1 cut(s) 588
BanI GGYRCC 1 cut(s) 469
BanII GRGCYC 1 cut(s) 349
Bbv12I GWGCWC 2 cut(s) 21, 218
BccI CCATC 2 cut(s) 419, 594
BceAI ACGGC 3 cut(s) 202, 208, 219
BcgI CGANNNNNNTGC 2 cut(s) 376, 410
BcnI CCSGG 2 cut(s) 75, 467
BcoDI GTCTC 2 cut(s) 555, 607
BfoI RGCGCY 1 cut(s) 212
BisI GCNGC 2 cut(s) 207, 367
BlpI GCTNAGC 1 cut(s) 212
BlsI GCNGC 2 cut(s) 208, 368
Bme1390I CCNGG 2 cut(s) 75, 467
Bme18I GGWCC 1 cut(s) 588
BmgT120I GGNCC 2 cut(s) 106, 588
BmiI GGNNCC 3 cut(s) 145, 471, 589
BmrFI CCNGG 2 cut(s) 75, 467
BmrI ACTGGG 1 cut(s) 631
BmsI GCATC 3 cut(s) 204, 588, 594
BmuI ACTGGG 1 cut(s) 631
BpmI CTGGAG 1 cut(s) 579
Bpu10I CCTNAGC 1 cut(s) 348
Bpu1102I GCTNAGC 1 cut(s) 212
BpuEI CTTGAG 1 cut(s) 404
BpuMI CCSGG 2 cut(s) 75, 467
BsaHI GRCGYC 1 cut(s) 135
BsaJI CCNNGG 1 cut(s) 147
BsaWI WCCGGW 2 cut(s) 140, 755
BsaXI ACNNNNNCTCC 2 cut(s) 552, 582
Bsc4I CCNNNNNNNGG 3 cut(s) 153, 342, 343
Bse118I RCCGGY 2 cut(s) 440, 551
Bse1I ACTGG 5 cut(s) 354, 601, 626, 638, 687
Bse3DI GCAATG 1 cut(s) 36
BseDI CCNNGG 1 cut(s) 147
BseGI GGATG 2 cut(s) 605, 609
BseLI CCNNNNNNNGG 3 cut(s) 153, 342, 343
BseMI GCAATG 1 cut(s) 36
BseMII CTCAG 3 cut(s) 203, 362, 419
BseNI ACTGG 5 cut(s) 354, 601, 626, 638, 687
BseRI GAGGAG 2 cut(s) 372, 501
Bsh1236I CGCG 1 cut(s) 438
Bsh1285I CGRYCG 1 cut(s) 697
BshFI GGCC 3 cut(s) 5, 107, 353
BshNI GGYRCC 1 cut(s) 469
BsiEI CGRYCG 1 cut(s) 697
BsiHKAI GWGCWC 2 cut(s) 21, 218
BsiSI CCGG 8 cut(s) 75, 87, 141, 441, 466, 478, 552, 756
BslFI GGGAC 2 cut(s) 434, 707
BslI CCNNNNNNNGG 3 cut(s) 153, 342, 343
BsmAI GTCTC 2 cut(s) 555, 607
BsmBI CGTCTC 2 cut(s) 555, 607
BsmFI GGGAC 2 cut(s) 434, 707
BsmI GAATGC 1 cut(s) 41
BsnI GGCC 3 cut(s) 5, 107, 353
Bsp1286I GDGCHC 3 cut(s) 21, 218, 349
Bsp1407I TGTACA 1 cut(s) 177
Bsp143I GATC 6 cut(s) 306, 397, 423, 492, 504, 537
Bsp1720I GCTNAGC 1 cut(s) 212
Bsp19I CCATGG 1 cut(s) 147
BspACI CCGC 6 cut(s) 90, 129, 206, 318, 366, 436
BspANI GGCC 3 cut(s) 5, 107, 353
BspCNI CTCAG 3 cut(s) 204, 361, 418
BspFNI CGCG 1 cut(s) 438
BspLI GGNNCC 3 cut(s) 145, 471, 589
BspPI GGATC 1 cut(s) 500
BspT107I GGYRCC 1 cut(s) 469
BsrDI GCAATG 1 cut(s) 36
BsrFI RCCGGY 2 cut(s) 440, 551
BsrGI TGTACA 1 cut(s) 177
BsrI ACTGG 5 cut(s) 354, 601, 626, 638, 687
BssAI RCCGGY 2 cut(s) 440, 551
BssECI CCNNGG 1 cut(s) 147
BssMI GATC 6 cut(s) 306, 397, 423, 492, 504, 537
BssNI GRCGYC 1 cut(s) 135
BssT1I CCWWGG 1 cut(s) 147
Bst4CI ACNGT 5 cut(s) 52, 236, 649, 663, 695
Bst6I CTCTTC 1 cut(s) 230
BstACI GRCGYC 1 cut(s) 135
BstAUI TGTACA 1 cut(s) 177
BstC8I GCNNGC 1 cut(s) 442
BstDEI CTNAG 3 cut(s) 212, 348, 405
BstDSI CCRYGG 1 cut(s) 147
BstF5I GGATG 2 cut(s) 605, 609
BstFNI CGCG 1 cut(s) 438
BstH2I RGCGCY 1 cut(s) 212
BstHHI GCGC 2 cut(s) 211, 440
BstKTI GATC 6 cut(s) 309, 400, 426, 495, 507, 540
BstMAI GTCTC 2 cut(s) 555, 607
BstMBI GATC 6 cut(s) 306, 397, 423, 492, 504, 537
BstMCI CGRYCG 1 cut(s) 697
BstMWI GCNNNNNNNGC 2 cut(s) 372, 634
BstNSI RCATGY 1 cut(s) 162
BstSCI CCNGG 2 cut(s) 73, 465
BstUI CGCG 1 cut(s) 438
BsuRI GGCC 3 cut(s) 5, 107, 353
BtgI CCRYGG 1 cut(s) 147
BtsCI GGATG 2 cut(s) 605, 609
BtsI GCAGTG 1 cut(s) 216
BtsIMutI CAGTG 4 cut(s) 216, 361, 682, 694
Cac8I GCNNGC 1 cut(s) 442
CaiI CAGNNNCTG 1 cut(s) 774
CfoI GCGC 2 cut(s) 211, 440
Cfr10I RCCGGY 2 cut(s) 440, 551
Cfr13I GGNCC 2 cut(s) 106, 588
CseI GACGC 2 cut(s) 71, 143
Csp6I GTAC 3 cut(s) 166, 178, 433
CviAII CATG 5 cut(s) 10, 110, 148, 159, 474
CviJI RGCY 9 cut(s) 5, 107, 189, 243, 251, 347, 353, 369, 409
CviKI_1 RGCY 9 cut(s) 5, 107, 189, 243, 251, 347, 353, 369, 409
CviQI GTAC 3 cut(s) 166, 178, 433
DdeI CTNAG 3 cut(s) 212, 348, 405
DpnI GATC 6 cut(s) 308, 399, 425, 494, 506, 539
DpnII GATC 6 cut(s) 306, 397, 423, 492, 504, 537
Eam1104I CTCTTC 1 cut(s) 230
EarI CTCTTC 1 cut(s) 230
EciI GGCGGA 1 cut(s) 105
Eco130I CCWWGG 1 cut(s) 147
Eco24I GRGCYC 1 cut(s) 349
Eco47I GGWCC 1 cut(s) 588
Eco57I CTGAAG 2 cut(s) 658, 751
EcoT14I CCWWGG 1 cut(s) 147
EcoT38I GRGCYC 1 cut(s) 349
ErhI CCWWGG 1 cut(s) 147
Esp3I CGTCTC 2 cut(s) 555, 607
FaeI CATG 5 cut(s) 13, 113, 151, 162, 477
FaiI YATR 7 cut(s) 11, 111, 123, 149, 160, 460, 475
FaqI GGGAC 2 cut(s) 434, 707
FatI CATG 5 cut(s) 9, 109, 147, 158, 473
FblI GTMKAC 1 cut(s) 298
Fnu4HI GCNGC 2 cut(s) 207, 367
FokI GGATG 2 cut(s) 612, 616
FriOI GRGCYC 1 cut(s) 349
Fsp4HI GCNGC 2 cut(s) 207, 367
GlaI GCGC 2 cut(s) 210, 439
GluI GCNGC 2 cut(s) 207, 367
GsuI CTGGAG 1 cut(s) 579
HaeII RGCGCY 1 cut(s) 212
HaeIII GGCC 3 cut(s) 5, 107, 353
HapII CCGG 8 cut(s) 75, 87, 141, 441, 466, 478, 552, 756
HgaI GACGC 2 cut(s) 71, 143
HhaI GCGC 2 cut(s) 211, 440
Hin1I GRCGYC 1 cut(s) 135
Hin1II CATG 5 cut(s) 13, 113, 151, 162, 477
Hin6I GCGC 2 cut(s) 209, 438
HinP1I GCGC 2 cut(s) 209, 438
HinfI GANTC 1 cut(s) 771
HpaII CCGG 8 cut(s) 75, 87, 141, 441, 466, 478, 552, 756
HphI GGTGA 5 cut(s) 71, 143, 307, 586, 745
Hpy166II GTNNAC 3 cut(s) 82, 274, 299
Hpy188I TCNGA 5 cut(s) 537, 643, 659, 770, 776
Hpy188III TCNNGA 2 cut(s) 421, 502
Hpy8I GTNNAC 3 cut(s) 82, 274, 299
Hpy99I CGWCG 2 cut(s) 188, 551
HpyAV CCTTC 4 cut(s) 16, 97, 682, 735
HpyCH4III ACNGT 5 cut(s) 52, 236, 649, 663, 695
HpyCH4IV ACGT 3 cut(s) 546, 564, 717
HpyCH4V TGCA 2 cut(s) 41, 375
HpyF10VI GCNNNNNNNGC 2 cut(s) 372, 634
HpyF3I CTNAG 3 cut(s) 212, 348, 405
HpySE526I ACGT 3 cut(s) 546, 564, 717
Hsp92I GRCGYC 1 cut(s) 135
Hsp92II CATG 5 cut(s) 13, 113, 151, 162, 477
HspAI GCGC 2 cut(s) 209, 438
KroI GCCGGC 1 cut(s) 440
KroNI GCCGGC 1 cut(s) 442
Kzo9I GATC 6 cut(s) 306, 397, 423, 492, 504, 537
LmnI GCTCC 1 cut(s) 248
LweI GCATC 3 cut(s) 204, 588, 594
MaeII ACGT 3 cut(s) 546, 564, 717
MaeIII GTNAC 6 cut(s) 59, 131, 287, 313, 356, 689
MalI GATC 6 cut(s) 308, 399, 425, 494, 506, 539
MboI GATC 6 cut(s) 306, 397, 423, 492, 504, 537
MboII GAAGA 2 cut(s) 112, 217
MfeI CAATTG 1 cut(s) 730
MhlI GDGCHC 3 cut(s) 21, 218, 349
MluCI AATT 4 cut(s) 261, 325, 340, 730
MmeI TCCRAC 2 cut(s) 567, 637
MnlI CCTC 8 cut(s) 44, 146, 233, 313, 390, 393, 479, 482
MroNI GCCGGC 1 cut(s) 440
MseI TTAA 1 cut(s) 264
MslI CAYNNNNRTG 1 cut(s) 14
MspI CCGG 8 cut(s) 75, 87, 141, 441, 466, 478, 552, 756
MspR9I CCNGG 2 cut(s) 75, 467
MunI CAATTG 1 cut(s) 730
Mva1269I GAATGC 1 cut(s) 41
MvnI CGCG 1 cut(s) 438
MwoI GCNNNNNNNGC 2 cut(s) 372, 634
NaeI GCCGGC 1 cut(s) 442
NciI CCSGG 2 cut(s) 75, 467
NcoI CCATGG 1 cut(s) 147
NdeII GATC 6 cut(s) 306, 397, 423, 492, 504, 537
NgoMIV GCCGGC 1 cut(s) 440
NlaIII CATG 5 cut(s) 13, 113, 151, 162, 477
NlaIV GGNNCC 3 cut(s) 145, 471, 589
NmeAIII GCCGAG 1 cut(s) 454
NmuCI GTSAC 6 cut(s) 59, 131, 287, 313, 356, 689
NspI RCATGY 1 cut(s) 162
PctI GAATGC 1 cut(s) 41
PdiI GCCGGC 1 cut(s) 442
PflFI GACNNNGTC 1 cut(s) 647
PkrI GCNGC 2 cut(s) 208, 368
Psp1406I AACGTT 1 cut(s) 717
PspN4I GGNNCC 3 cut(s) 145, 471, 589
PspPI GGNCC 2 cut(s) 106, 588
PstNI CAGNNNCTG 1 cut(s) 774
PsyI GACNNNGTC 1 cut(s) 647
RsaI GTAC 3 cut(s) 167, 179, 434
RsaNI GTAC 3 cut(s) 166, 178, 433
RseI CAYNNNNRTG 1 cut(s) 14
SaqAI TTAA 1 cut(s) 264
SatI GCNGC 2 cut(s) 207, 367
Sau3AI GATC 6 cut(s) 306, 397, 423, 492, 504, 537
Sau96I GGNCC 2 cut(s) 106, 588
ScrFI CCNGG 2 cut(s) 75, 467
SduI GDGCHC 3 cut(s) 21, 218, 349
SetI ASST 6 cut(s) 245, 253, 500, 549, 567, 720
SfaNI GCATC 3 cut(s) 204, 588, 594
SgrAI CRCCGGYG 1 cut(s) 551
SinI GGWCC 1 cut(s) 588
SmiMI CAYNNNNRTG 1 cut(s) 14
SmlI CTYRAG 1 cut(s) 419
SmoI CTYRAG 1 cut(s) 419
Sse9I AATT 4 cut(s) 261, 325, 340, 730
SsiI CCGC 6 cut(s) 90, 129, 206, 318, 366, 436
StyD4I CCNGG 2 cut(s) 73, 465
StyI CCWWGG 1 cut(s) 147
TaaI ACNGT 5 cut(s) 52, 236, 649, 663, 695
TaiI ACGT 3 cut(s) 549, 567, 720
TaqI TCGA 2 cut(s) 305, 396
TasI AATT 4 cut(s) 261, 325, 340, 730
TatI WGTACW 1 cut(s) 177
TauI GCSGC 2 cut(s) 209, 369
Tru1I TTAA 1 cut(s) 264
Tru9I TTAA 1 cut(s) 264
TscAI CASTG 4 cut(s) 223, 361, 682, 694
TseFI GTSAC 6 cut(s) 59, 131, 287, 313, 356, 689
Tsp45I GTSAC 6 cut(s) 59, 131, 287, 313, 356, 689
TspGWI ACGGA 2 cut(s) 183, 631
TspRI CASTG 4 cut(s) 223, 361, 682, 694
Tth111I GACNNNGTC 1 cut(s) 647
VpaK11BI GGWCC 1 cut(s) 588
XceI RCATGY 1 cut(s) 162
XcmI CCANNNNNNNNNTGG 1 cut(s) 145
XmiI GTMKAC 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.