Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0000971

Physical Location & Seq

Genomic Coordinates Forward (+)

21044317 .. 21046346

Gene Structure 2030 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G56840.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1398 bp

ATGAAGCATAAACCTGAAACGGCGGCGTTTAGCTTGATTCGGCCTCTCGAAGCTTGGGCTGCAAATGCAAACAAAGCCTGTAATACTAAGAAGATGCTACCTTGTCTGGGTAGAAAATGGATGAGATTGTCAACGAGGAATCTAAAACCAACTTGGAACTTGATAAACGTCGTTGACGCAAGTAAAACAATTGTGAGAGGAATAAGCGGTGGAGCTGAAACGATAGCTAAAGAGAGAGTTGACACCGTAGTGATCGGTGCCGGAGTCGTAGGTTTAGCGGTGGCGCGTGAGCTCAGTCTCCGCGGCAGAGAAGTTCTGATCCTCGACGCCGCATCATCGTTCGGCACCGTGACGAGTTCCCGCAACAGTGAAGTCGTCCACGCCGGAATCTATTATCCACCAAACTCTCTAAAGGCCAAGTTTTGTGTAAGAGGGAGGGAATTGCTGTATAAATACTGCTCTGAATATGAAATCCCTCATAAGAAGATTGGTAAGCTTATCGTTGCTACGGGATCCTCTGAGATACCGAAACTAGATCTATTGATGCATCTTGGAACTCAGAATAGAGTCTCGGGTCTAAGGATGTTGGAAGGTTTTGAAGCTATGAGAATGGAGCCACAGCTTCGTTGTGTTAAAGCTTTGCTATCTCCTGAATCTGGCATTCTTGATACACATTCCTTCATGCTATCTTTAGTGGGAGAAGCACAGAACAACCACGCAACTTTCTCGTACAACACGGTGGTTTTAAACGGTCGTGTTGAAGAAAAGAAGATGCATTTGTATGTTGCTGATACCAGATTTTCTGAATCTCGGTGTGAGGCAGAAGCACAGCTTGAGCTTATCCCTAATCTTGTTGTTAACTCTGCGGGATTAGGTGCTCAAGCTCTAGCAAAGAGACTACATGGCTTAGATCATCGATTTGTTCCTTCGTCTCACTATGCTCGTGGATGCTACTTTACACTATCGGGCATAAAGGCTCCACCTTTTAATAAACTTGTGTATCCTATACCTGAGGAAGGAGGTCTCGGTGTCCATGTCACTGTAGATTTGAACGGACTTGTTAAGTTTGGACCTGATGTAGAATGGATTGAATGCACAGATGATACATCAAGCTTCCTCAACAAGTTTGATTATCGTGTAAACCCACAACGATCAGAGAAATTCTACCCAGAGATCAGAAAGTACTATCCGGATCTCAAAGATGGATCATTGGAACCGGGTTATTCAGGCATCCGTCCTAAGCTTTCCGGGCCAAAACAGTCTCCTGCAGATTTTGTCATACAGGGAGAGGAGACTCATGGAGTTCCTGGTCTTGTTAATCTCTTTGGCATCGAATCACCTGGTTTAACTTCAAGCTTGGCTATTGCAGAACATATAGCAAACAAATTCTTGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0003973 GO:0005488 GO:0008150 GO:0008152 GO:0016491 GO:0016614 GO:0016899 GO:0036094 GO:0043167 GO:0043168 GO:0047545 GO:0048037 GO:0050660 GO:0050662 GO:0055114 GO:0071949 GO:0097159 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DAO

Protein Analysis

465

Amino Acids

51.25

Weight (kDa)

8.99

Isoelectric Point (pI)

40.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAO	PF01266	81 - 461	1.2e-56	FAD dependent oxidoreductase
NAD_binding_8	PF13450	84 - 122	2.2e-06	NAD(P)-binding Rossmann-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000971)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G56840
fragaria_vesca	FvH4_1g29380 FvH4_1g29380 FvH4_1g29380 FvH4_1g29380 FvH4_1g29380 FvH4_1g29380 FvH4_1g29380 FvH4_1g29380
malus_domestica	MD14G1091600.v1.1 MD14G1100100.v1.1
prunus_persica	Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1
pyrus_communis	pycom14g09020
rosa_chinensis	RchiOBHm_Chr3g0495031
rosa_laevigata	RLG00000022654
rosa_multiflora	Rmu_ssc0000058.1_g000010
rosa_roxburghii	Rroxscaffold_6G00390830
rosa_rugosa	Rorug03G0269400
rosa_samantha	Rh3AG318400 Rh3BG353500 Rh3CG350400 Rh3DG352800
rosa_wichuraiana	Rw0G014470 Rw0G015070 Rw3G027760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	257, 344
AccII	CGCG	2 cut(s)	286, 303
AccIII	TCCGGA	1 cut(s)	1189
AciI	CCGC	8 cut(s)	23, 207, 278, 301, 303, 330, 361, 866
AclWI	GGATC	5 cut(s)	313, 507, 520, 1200, 1213
AcsI	RAATTY	2 cut(s)	1160, 1385
AcyI	GRCGYC	1 cut(s)	327
AfaI	GTAC	2 cut(s)	731, 1184
AfiI	CCNNNNNNNGG	3 cut(s)	107, 656, 1016
AgsI	TTSAA	5 cut(s)	599, 761, 1051, 1091, 1353
AjnI	CCWGG	2 cut(s)	1306, 1339
AleI	CACNNNNGTG	1 cut(s)	248
Alw21I	GWGCWC	2 cut(s)	294, 880
Alw26I	GTCTC	7 cut(s)	302, 574, 889, 936, 1028, 1266, 1286
AlwI	GGATC	5 cut(s)	313, 507, 520, 1200, 1213
Ama87I	CYCGRG	1 cut(s)	571
Aor13HI	TCCGGA	1 cut(s)	1189
AoxI	GGCC	3 cut(s)	41, 414, 1250
ApeKI	GCWGC	1 cut(s)	59
ApoI	RAATTY	2 cut(s)	1160, 1385
AspLEI	GCGC	1 cut(s)	286
AspS9I	GGNCC	2 cut(s)	1070, 1250
AsuC2I	CCSGG	2 cut(s)	1218, 1249
AsuHPI	GGTGA	1 cut(s)	1329
AvaI	CYCGRG	1 cut(s)	571
AvaII	GGWCC	1 cut(s)	1070
AxyI	CCTNAGG	1 cut(s)	1011
BaeI	ACNNNNGTAYC	2 cut(s)	983, 1016
BamHI	GGATCC	1 cut(s)	512
BanI	GGYRCC	2 cut(s)	257, 344
BanII	GRGCYC	1 cut(s)	294
BauI	CACGAG	1 cut(s)	942
Bbv12I	GWGCWC	2 cut(s)	294, 880
BbvI	GCAGC	1 cut(s)	46
BccI	CCATC	1 cut(s)	1196
BceAI	ACGGC	1 cut(s)	36
BcgI	CGANNNNNNTGC	2 cut(s)	708, 742
BciT130I	CCWGG	2 cut(s)	1308, 1341
BciVI	GTATCC	1 cut(s)	1011
BcnI	CCSGG	2 cut(s)	1218, 1249
BcoDI	GTCTC	7 cut(s)	302, 574, 889, 936, 1028, 1266, 1286
BfaI	CTAG	2 cut(s)	533, 887
BfmI	CTRYAG	2 cut(s)	1041, 1266
BfuI	GTATCC	1 cut(s)	1011
BglII	AGATCT	1 cut(s)	535
BisI	GCNGC	4 cut(s)	24, 60, 304, 330
BlsI	GCNGC	4 cut(s)	25, 61, 305, 331
BmcAI	AGTACT	1 cut(s)	1184
Bme1390I	CCNGG	4 cut(s)	1218, 1249, 1308, 1341
Bme18I	GGWCC	1 cut(s)	1070
BmeT110I	CYCGRG	1 cut(s)	571
BmgT120I	GGNCC	2 cut(s)	1070, 1250
BmiI	GGNNCC	6 cut(s)	259, 346, 514, 615, 978, 1215
BmrFI	CCNGG	4 cut(s)	1218, 1249, 1308, 1341
BmsI	GCATC	8 cut(s)	84, 341, 534, 556, 762, 938, 1239, 1338
Bpu10I	CCTNAGC	1 cut(s)	1239
BpuEI	CTTGAG	2 cut(s)	854, 864
BpuMI	CCSGG	2 cut(s)	1218, 1249
Bsa29I	ATCGAT	1 cut(s)	916
BsaHI	GRCGYC	1 cut(s)	327
BsaI	GGTCTC	1 cut(s)	1028
BsaJI	CCNNGG	1 cut(s)	301
BsaWI	WCCGGW	1 cut(s)	1189
Bsc4I	CCNNNNNNNGG	3 cut(s)	107, 656, 1016
Bse21I	CCTNAGG	1 cut(s)	1011
BseAI	TCCGGA	1 cut(s)	1189
BseBI	CCWGG	2 cut(s)	1308, 1341
BseCI	ATCGAT	1 cut(s)	916
BseDI	CCNNGG	1 cut(s)	301
BseGI	GGATG	4 cut(s)	126, 588, 953, 1230
BseLI	CCNNNNNNNGG	3 cut(s)	107, 656, 1016
BseMII	CTCAG	4 cut(s)	307, 510, 572, 1002
BseRI	GAGGAG	1 cut(s)	1304
BseXI	GCAGC	1 cut(s)	46
Bsh1236I	CGCG	2 cut(s)	286, 303
Bsh1285I	CGRYCG	1 cut(s)	754
BshFI	GGCC	3 cut(s)	43, 416, 1252
BshNI	GGYRCC	2 cut(s)	257, 344
BshVI	ATCGAT	1 cut(s)	916
BsiEI	CGRYCG	1 cut(s)	754
BsiHKAI	GWGCWC	2 cut(s)	294, 880
BsiHKCI	CYCGRG	1 cut(s)	571
BsiSI	CCGG	5 cut(s)	261, 384, 1190, 1217, 1248
BslI	CCNNNNNNNGG	3 cut(s)	107, 656, 1016
BsmAI	GTCTC	7 cut(s)	302, 574, 889, 936, 1028, 1266, 1286
BsmBI	CGTCTC	1 cut(s)	936
BsmI	GAATGC	2 cut(s)	660, 1097
BsnI	GGCC	3 cut(s)	43, 416, 1252
Bso31I	GGTCTC	1 cut(s)	1028
BsoBI	CYCGRG	1 cut(s)	571
Bsp1286I	GDGCHC	2 cut(s)	294, 880
Bsp13I	TCCGGA	1 cut(s)	1189
Bsp143I	GATC	9 cut(s)	252, 318, 512, 535, 910, 1151, 1173, 1192, 1205
BspACI	CCGC	8 cut(s)	23, 207, 278, 301, 303, 330, 361, 866
BspANI	GGCC	3 cut(s)	43, 416, 1252
BspCNI	CTCAG	4 cut(s)	306, 511, 571, 1003
BspDI	ATCGAT	1 cut(s)	916
BspEI	TCCGGA	1 cut(s)	1189
BspFNI	CGCG	2 cut(s)	286, 303
BspLI	GGNNCC	6 cut(s)	259, 346, 514, 615, 978, 1215
BspMAI	CTGCAG	1 cut(s)	1270
BspPI	GGATC	5 cut(s)	313, 507, 520, 1200, 1213
BspT107I	GGYRCC	2 cut(s)	257, 344
BspTNI	GGTCTC	1 cut(s)	1028
BssECI	CCNNGG	1 cut(s)	301
BssMI	GATC	9 cut(s)	252, 318, 512, 535, 910, 1151, 1173, 1192, 1205
BssNI	GRCGYC	1 cut(s)	327
BssSI	CACGAG	1 cut(s)	942
Bst2BI	CACGAG	1 cut(s)	942
Bst2UI	CCWGG	2 cut(s)	1308, 1341
Bst4CI	ACNGT	7 cut(s)	247, 349, 368, 739, 752, 1042, 1260
BstACI	GRCGYC	1 cut(s)	327
BstDEI	CTNAG	8 cut(s)	87, 293, 519, 558, 578, 907, 1011, 1239
BstDSI	CCRYGG	1 cut(s)	301
BstENI	CCTNNNNNAGG	1 cut(s)	1014
BstF5I	GGATG	4 cut(s)	126, 588, 953, 1230
BstFNI	CGCG	2 cut(s)	286, 303
BstHHI	GCGC	1 cut(s)	286
BstKTI	GATC	9 cut(s)	255, 321, 515, 538, 913, 1154, 1176, 1195, 1208
BstMAI	GTCTC	7 cut(s)	302, 574, 889, 936, 1028, 1266, 1286
BstMBI	GATC	9 cut(s)	252, 318, 512, 535, 910, 1151, 1173, 1192, 1205
BstMCI	CGRYCG	1 cut(s)	754
BstMWI	GCNNNNNNNGC	4 cut(s)	59, 65, 74, 1249
BstNI	CCWGG	2 cut(s)	1308, 1341
BstSCI	CCNGG	4 cut(s)	1216, 1247, 1306, 1339
BstSFI	CTRYAG	2 cut(s)	1041, 1266
BstUI	CGCG	2 cut(s)	286, 303
BstV1I	GCAGC	1 cut(s)	46
BstX2I	RGATCY	3 cut(s)	512, 535, 1192
BstYI	RGATCY	3 cut(s)	512, 535, 1192
Bsu15I	ATCGAT	1 cut(s)	916
Bsu36I	CCTNAGG	1 cut(s)	1011
BsuI	GTATCC	1 cut(s)	1011
BsuRI	GGCC	3 cut(s)	43, 416, 1252
BsuTUI	ATCGAT	1 cut(s)	916
BtgI	CCRYGG	1 cut(s)	301
BtsCI	GGATG	4 cut(s)	126, 588, 953, 1230
BtsIMutI	CAGTG	2 cut(s)	373, 1038
CfoI	GCGC	1 cut(s)	286
Cfr13I	GGNCC	2 cut(s)	1070, 1250
Cfr42I	CCGCGG	1 cut(s)	304
ClaI	ATCGAT	1 cut(s)	916
CseI	GACGC	2 cut(s)	185, 335
CsiI	ACCWGGT	1 cut(s)	1339
Csp6I	GTAC	2 cut(s)	730, 1183
CviAII	CATG	4 cut(s)	682, 902, 1034, 1298
CviQI	GTAC	2 cut(s)	730, 1183
DdeI	CTNAG	8 cut(s)	87, 293, 519, 558, 578, 907, 1011, 1239
DpnI	GATC	9 cut(s)	254, 320, 514, 537, 912, 1153, 1175, 1194, 1207
DpnII	GATC	9 cut(s)	252, 318, 512, 535, 910, 1151, 1173, 1192, 1205
DraI	TTTAAA	1 cut(s)	747
Ecl136II	GAGCTC	1 cut(s)	292
Eco24I	GRGCYC	1 cut(s)	294
Eco31I	GGTCTC	1 cut(s)	1028
Eco47I	GGWCC	1 cut(s)	1070
Eco53kI	GAGCTC	1 cut(s)	292
Eco81I	CCTNAGG	1 cut(s)	1011
Eco88I	CYCGRG	1 cut(s)	571
EcoICRI	GAGCTC	1 cut(s)	292
EcoNI	CCTNNNNNAGG	1 cut(s)	1014
EcoRII	CCWGG	2 cut(s)	1306, 1339
EcoT22I	ATGCAT	2 cut(s)	549, 777
EcoT38I	GRGCYC	1 cut(s)	294
Esp3I	CGTCTC	1 cut(s)	936
FaeI	CATG	4 cut(s)	685, 905, 1037, 1301
FalI	AAGNNNNNCTT	2 cut(s)	816, 848
FatI	CATG	4 cut(s)	681, 901, 1033, 1297
FauI	CCCGC	2 cut(s)	368, 859
Fnu4HI	GCNGC	4 cut(s)	24, 60, 304, 330
FokI	GGATG	4 cut(s)	133, 595, 960, 1217
FriOI	GRGCYC	1 cut(s)	294
Fsp4HI	GCNGC	4 cut(s)	24, 60, 304, 330
FspBI	CTAG	2 cut(s)	533, 887
GlaI	GCGC	1 cut(s)	285
GluI	GCNGC	4 cut(s)	24, 60, 304, 330
HaeIII	GGCC	3 cut(s)	43, 416, 1252
HapII	CCGG	5 cut(s)	261, 384, 1190, 1217, 1248
HgaI	GACGC	2 cut(s)	185, 335
HhaI	GCGC	1 cut(s)	286
Hin1I	GRCGYC	1 cut(s)	327
Hin1II	CATG	4 cut(s)	685, 905, 1037, 1301
Hin6I	GCGC	1 cut(s)	284
HinP1I	GCGC	1 cut(s)	284
HincII	GTYRAC	4 cut(s)	132, 175, 241, 859
HindII	GTYRAC	4 cut(s)	132, 175, 241, 859
HindIII	AAGCTT	6 cut(s)	51, 494, 636, 1111, 1241, 1354
HinfI	GANTC	9 cut(s)	37, 139, 264, 387, 567, 653, 806, 1294, 1334
HpaI	GTTAAC	1 cut(s)	859
HpaII	CCGG	5 cut(s)	261, 384, 1190, 1217, 1248
HphI	GGTGA	1 cut(s)	1329
Hpy166II	GTNNAC	6 cut(s)	132, 175, 241, 379, 859, 1141
Hpy188I	TCNGA	7 cut(s)	318, 463, 520, 561, 805, 1156, 1178
Hpy188III	TCNNGA	5 cut(s)	47, 650, 665, 1190, 1390
Hpy8I	GTNNAC	6 cut(s)	132, 175, 241, 379, 859, 1141
Hpy99I	CGWCG	2 cut(s)	173, 329
HpyAV	CCTTC	4 cut(s)	584, 688, 936, 1010
HpyCH4III	ACNGT	7 cut(s)	247, 349, 368, 739, 752, 1042, 1260
HpyCH4IV	ACGT	1 cut(s)	168
HpyCH4V	TGCA	7 cut(s)	62, 68, 547, 775, 1095, 1268, 1367
HpyF10VI	GCNNNNNNNGC	4 cut(s)	59, 65, 74, 1249
HpyF3I	CTNAG	8 cut(s)	87, 293, 519, 558, 578, 907, 1011, 1239
HpySE526I	ACGT	1 cut(s)	168
Hsp92I	GRCGYC	1 cut(s)	327
Hsp92II	CATG	4 cut(s)	685, 905, 1037, 1301
HspAI	GCGC	1 cut(s)	284
Kpn2I	TCCGGA	1 cut(s)	1189
KspAI	GTTAAC	1 cut(s)	859
KspI	CCGCGG	1 cut(s)	304
Kzo9I	GATC	9 cut(s)	252, 318, 512, 535, 910, 1151, 1173, 1192, 1205
LmnI	GCTCC	3 cut(s)	212, 613, 982
Lsp1109I	GCAGC	1 cut(s)	46
LweI	GCATC	8 cut(s)	84, 341, 534, 556, 762, 938, 1239, 1338
MabI	ACCWGGT	1 cut(s)	1339
MaeI	CTAG	2 cut(s)	533, 887
MaeII	ACGT	1 cut(s)	168
MaeIII	GTNAC	2 cut(s)	349, 1036
MalI	GATC	9 cut(s)	254, 320, 514, 537, 912, 1153, 1175, 1194, 1207
MboI	GATC	9 cut(s)	252, 318, 512, 535, 910, 1151, 1173, 1192, 1205
MboII	GAAGA	4 cut(s)	103, 496, 773, 781
MfeI	CAATTG	1 cut(s)	189
MflI	RGATCY	3 cut(s)	512, 535, 1192
MhlI	GDGCHC	2 cut(s)	294, 880
MluCI	AATT	4 cut(s)	189, 440, 1160, 1385
MlyI	GAGTC	3 cut(s)	273, 576, 1288
MmeI	TCCRAC	1 cut(s)	567
Mph1103I	ATGCAT	2 cut(s)	549, 777
MroI	TCCGGA	1 cut(s)	1189
MseI	TTAA	7 cut(s)	633, 746, 858, 987, 1062, 1317, 1346
MslI	CAYNNNNRTG	2 cut(s)	248, 780
MspA1I	CMGCKG	1 cut(s)	303
MspI	CCGG	5 cut(s)	261, 384, 1190, 1217, 1248
MspR9I	CCNGG	4 cut(s)	1218, 1249, 1308, 1341
MunI	CAATTG	1 cut(s)	189
Mva1269I	GAATGC	2 cut(s)	660, 1097
MvaI	CCWGG	2 cut(s)	1308, 1341
MvnI	CGCG	2 cut(s)	286, 303
MwoI	GCNNNNNNNGC	4 cut(s)	59, 65, 74, 1249
NciI	CCSGG	2 cut(s)	1218, 1249
NdeII	GATC	9 cut(s)	252, 318, 512, 535, 910, 1151, 1173, 1192, 1205
NlaIII	CATG	4 cut(s)	685, 905, 1037, 1301
NlaIV	GGNNCC	6 cut(s)	259, 346, 514, 615, 978, 1215
NmuCI	GTSAC	2 cut(s)	349, 1036
NsiI	ATGCAT	2 cut(s)	549, 777
OliI	CACNNNNGTG	1 cut(s)	248
PctI	GAATGC	2 cut(s)	660, 1097
PfeI	GAWTC	6 cut(s)	37, 139, 387, 653, 806, 1334
PkrI	GCNGC	4 cut(s)	25, 61, 305, 331
PleI	GAGTC	3 cut(s)	272, 575, 1288
PpsI	GAGTC	3 cut(s)	272, 575, 1288
Psp124BI	GAGCTC	1 cut(s)	294
Psp6I	CCWGG	2 cut(s)	1306, 1339
PspGI	CCWGG	2 cut(s)	1306, 1339
PspN4I	GGNNCC	6 cut(s)	259, 346, 514, 615, 978, 1215
PspPI	GGNCC	2 cut(s)	1070, 1250
PstI	CTGCAG	1 cut(s)	1270
PsuI	RGATCY	3 cut(s)	512, 535, 1192
RsaI	GTAC	2 cut(s)	731, 1184
RsaNI	GTAC	2 cut(s)	730, 1183
RseI	CAYNNNNRTG	2 cut(s)	248, 780
SacI	GAGCTC	1 cut(s)	294
SacII	CCGCGG	1 cut(s)	304
SaqAI	TTAA	7 cut(s)	633, 746, 858, 987, 1062, 1317, 1346
SatI	GCNGC	4 cut(s)	24, 60, 304, 330
Sau3AI	GATC	9 cut(s)	252, 318, 512, 535, 910, 1151, 1173, 1192, 1205
Sau96I	GGNCC	2 cut(s)	1070, 1250
ScaI	AGTACT	1 cut(s)	1184
SchI	GAGTC	3 cut(s)	273, 576, 1288
ScrFI	CCNGG	4 cut(s)	1218, 1249, 1308, 1341
SduI	GDGCHC	2 cut(s)	294, 880
SexAI	ACCWGGT	1 cut(s)	1339
SfaNI	GCATC	8 cut(s)	84, 341, 534, 556, 762, 938, 1239, 1338
SfcI	CTRYAG	2 cut(s)	1041, 1266
Sfr303I	CCGCGG	1 cut(s)	304
SgrBI	CCGCGG	1 cut(s)	304
SinI	GGWCC	1 cut(s)	1070
SmiMI	CAYNNNNRTG	2 cut(s)	248, 780
SmlI	CTYRAG	3 cut(s)	833, 879, 1390
SmoI	CTYRAG	3 cut(s)	833, 879, 1390
Sse9I	AATT	4 cut(s)	189, 440, 1160, 1385
SsiI	CCGC	8 cut(s)	23, 207, 278, 301, 303, 330, 361, 866
SspMI	CTAG	2 cut(s)	533, 887
SstI	GAGCTC	1 cut(s)	294
StyD4I	CCNGG	4 cut(s)	1216, 1247, 1306, 1339
TaaI	ACNGT	7 cut(s)	247, 349, 368, 739, 752, 1042, 1260
TaiI	ACGT	1 cut(s)	171
TaqI	TCGA	4 cut(s)	48, 324, 916, 1332
TasI	AATT	4 cut(s)	189, 440, 1160, 1385
TatI	WGTACW	1 cut(s)	1182
TauI	GCSGC	3 cut(s)	26, 306, 332
TfiI	GAWTC	6 cut(s)	37, 139, 387, 653, 806, 1334
Tru1I	TTAA	7 cut(s)	633, 746, 858, 987, 1062, 1317, 1346
Tru9I	TTAA	7 cut(s)	633, 746, 858, 987, 1062, 1317, 1346
TscAI	CASTG	2 cut(s)	373, 1045
TseFI	GTSAC	2 cut(s)	349, 1036
TseI	GCWGC	1 cut(s)	59
Tsp45I	GTSAC	2 cut(s)	349, 1036
TspDTI	ATGAA	3 cut(s)	17, 483, 670
TspGWI	ACGGA	2 cut(s)	1068, 1223
TspRI	CASTG	2 cut(s)	373, 1045
VpaK11BI	GGWCC	1 cut(s)	1070
XagI	CCTNNNNNAGG	1 cut(s)	1014
XapI	RAATTY	2 cut(s)	1160, 1385
XspI	CTAG	2 cut(s)	533, 887
ZrmI	AGTACT	1 cut(s)	1184
Zsp2I	ATGCAT	2 cut(s)	549, 777

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.