FvH4_1g29380

L-2-hydroxyglutarate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000971
Physical Location & Seq
Reverse (-)
22367062 .. 22370989
3928 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g29380.t2

Sequence Viewer

Length: 1260 bp
ATGGCGATGCAGAAGTGGTGGTGGTGGGCATCGAAGAGAGTGTGGAATCGTAATTACTGTATGACTAGTGGAGTGGCGAGAGAGAGAGTAGAGTGCGTGGTGATCGGAGCAGGTGTAGTGGGATTATGTGTGGCTAGAGAGCTGGCTTTAAGAGGTCGACAGGTCTTGGTCTTAGACTCGGCTCCCACCTTCGGCACTGGCACCAGTTCCCGCAACAGCGAAGTCATTCATGCCGGCATCTACTACCCTCCTAATTCTCTCAAGGCAATCTTTTGTGTGAGAGGAAGACAACTGCTGTACCAATATTGCTCTGAGCGCCAAGTTCCTCACAAGCAAATTGGTAAACTAATTGTTGCCACCGGATCTTCTGAGATTCACAAGTTGCATTATTTAATGGATTGTGGGATTCAAAATGGGGTTGATGGTCTGGTAATGATGGAGGGCTCCCAAGCCATGAGAATAGAACCCGAATTGAGATGTTCGAAAGCCTTACTATCACCAGTTTCTGGGATTGTCGATACACATTCTTTCATGCTTTCTCTGGTGGGTGATGCTGAGAATTGTGGCACAATCTTCTCCTACAACACTGCTGTTATTGGTGGCCACGTAGAACAAGATCAATTGTGCCTTCATGTTTCTCAAACCAAACATCTTGAAATGTGCAATGGAACTTCTCCGCTGCAACCAGAGATGGTACTCATTCCTAAGCTTGTTGTGAACTCTGCTGGGCTGAGTGCCCCTGCCCTTGCCAAGCGATTTGATGGCCTCCATAGTGCACTCATTCCCACCCCACATTATGCTCGCGGGTGCTACTTCACTCTATCCAATACTCAAATCTGTCCTTTCAAACATTTAATATATCCCGTACCAGAGGACGGTGGCCTTGGAGTGCATGTTACTTTGGATTTAAATGGTCAGCTCAAGTTTGGTCCAAATGTTGAATGGATCGATGGTATCGACGACGTTTCAAGCTTCCTGAATAAGTTTGACTATTCTGTATGCATTAGTCACATGAAGTTATTTTACCATGAGATAAAGAAGTACTACCCGAATCTAAAAGATGGGTCCCTCGAGCCAGGTTATGCAGGGATTAGACCGAAGCTTTCAGGTCCTAGACAATCTCCAGTTGATTTTGTGATACAGGGAGAGGATATTCATGGGATAACCGGTCTTGTCAACCTTTTTGGAATCGAGTCACCTGGTTTGACTTCAAGCATGGCAATTGCCGAGCACATAGCCATCAATTTCTTTAGATGTTGA

Protein Analysis

420

Amino Acids

46.16

Weight (kDa)

7.22

Isoelectric Point (pI)

39.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 32 - 321 2.4e-39 FAD dependent oxidoreductase
NAD_binding_8 PF13450 34 - 72 1.9e-06 NAD(P)-binding Rossmann-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 101
Acc36I ACCTGC 1 cut(s) 101
AccB1I GGYRCC 1 cut(s) 200
AccB7I CCANNNNNTGG 1 cut(s) 506
AccI GTMKAC 1 cut(s) 157
AccII CGCG 1 cut(s) 804
AciI CCGC 3 cut(s) 211, 677, 804
AclWI GGATC 2 cut(s) 370, 953
AcoI YGGCCR 1 cut(s) 601
AfaI GTAC 4 cut(s) 299, 696, 867, 1043
AfiI CCNNNNNNNGG 3 cut(s) 191, 506, 875
AgeI ACCGGT 1 cut(s) 1166
AgsI TTSAA 6 cut(s) 410, 656, 847, 941, 969, 1212
AhlI ACTAGT 1 cut(s) 65
AjnI CCWGG 2 cut(s) 1075, 1198
AluBI AGCT 5 cut(s) 142, 709, 919, 972, 1102
AluI AGCT 5 cut(s) 142, 709, 919, 972, 1102
Alw21I GWGCWC 2 cut(s) 778, 1233
Alw44I GTGCAC 1 cut(s) 774
AlwI GGATC 2 cut(s) 370, 953
AlwNI CAGNNNCTG 1 cut(s) 506
Ama87I CYCGRG 1 cut(s) 1070
AoxI GGCC 3 cut(s) 601, 763, 880
ApaLI GTGCAC 1 cut(s) 774
ApeKI GCWGC 1 cut(s) 679
AsiGI ACCGGT 1 cut(s) 1166
Asp700I GAANNNNTTC 1 cut(s) 225
AspLEI GCGC 1 cut(s) 318
AspS9I GGNCC 3 cut(s) 929, 1065, 1109
AsuHPI GGTGA 4 cut(s) 112, 489, 560, 1188
AsuII TTCGAA 1 cut(s) 482
AvaI CYCGRG 1 cut(s) 1070
AvaII GGWCC 3 cut(s) 929, 1065, 1109
BaeGI GKGCMC 2 cut(s) 739, 778
BaeI ACNNNNGTAYC 2 cut(s) 281, 314
BalI TGGCCA 1 cut(s) 603
BanI GGYRCC 1 cut(s) 200
BanII GRGCYC 1 cut(s) 446
BarI GAAGNNNNNNTAC 2 cut(s) 1007, 1039
BbsI GAAGAC 1 cut(s) 292
Bbv12I GWGCWC 2 cut(s) 778, 1233
BbvI GCAGC 1 cut(s) 666
BccI CCATC 7 cut(s) 416, 430, 685, 755, 944, 1055, 1247
BciT130I CCWGG 2 cut(s) 1077, 1200
BcuI ACTAGT 1 cut(s) 65
BfaI CTAG 3 cut(s) 66, 135, 1113
BfoI RGCGCY 1 cut(s) 319
BfuAI ACCTGC 1 cut(s) 101
BisI GCNGC 1 cut(s) 680
BlsI GCNGC 1 cut(s) 681
BmcAI AGTACT 1 cut(s) 1043
Bme1390I CCNGG 2 cut(s) 1077, 1200
Bme18I GGWCC 3 cut(s) 929, 1065, 1109
BmeT110I CYCGRG 1 cut(s) 1070
BmgT120I GGNCC 3 cut(s) 929, 1065, 1109
BmiI GGNNCC 5 cut(s) 183, 202, 445, 1066, 1067
BmrFI CCNGG 2 cut(s) 1077, 1200
BmsI GCATC 3 cut(s) 38, 246, 541
BpiI GAAGAC 1 cut(s) 292
BpmI CTGGAG 1 cut(s) 1107
Bpu10I CCTNAGC 1 cut(s) 705
Bpu14I TTCGAA 1 cut(s) 482
BpuEI CTTGAG 2 cut(s) 245, 905
Bsa29I ATCGAT 1 cut(s) 948
BsaAI YACGTR 1 cut(s) 607
BsaJI CCNNGG 1 cut(s) 883
BsaWI WCCGGW 2 cut(s) 359, 1166
BsaXI ACNNNNNCTCC 4 cut(s) 99, 129, 879, 909
Bsc4I CCNNNNNNNGG 3 cut(s) 191, 506, 875
Bse118I RCCGGY 2 cut(s) 233, 1166
Bse1I ACTGG 4 cut(s) 202, 204, 500, 1124
Bse3DI GCAATG 1 cut(s) 670
BseBI CCWGG 2 cut(s) 1077, 1200
BseCI ATCGAT 1 cut(s) 948
BseDI CCNNGG 1 cut(s) 883
BseLI CCNNNNNNNGG 3 cut(s) 191, 506, 875
BseMI GCAATG 1 cut(s) 670
BseMII CTCAG 4 cut(s) 303, 360, 546, 722
BseNI ACTGG 4 cut(s) 202, 204, 500, 1124
BseSI GKGCMC 2 cut(s) 739, 778
BseXI GCAGC 1 cut(s) 666
BseYI CCCAGC 1 cut(s) 725
Bsh1236I CGCG 1 cut(s) 804
BshFI GGCC 3 cut(s) 603, 765, 882
BshNI GGYRCC 1 cut(s) 200
BshTI ACCGGT 1 cut(s) 1166
BshVI ATCGAT 1 cut(s) 948
BsiHKAI GWGCWC 2 cut(s) 778, 1233
BsiHKCI CYCGRG 1 cut(s) 1070
BsiSI CCGG 3 cut(s) 234, 360, 1167
BslFI GGGAC 1 cut(s) 1051
BslI CCNNNNNNNGG 3 cut(s) 191, 506, 875
BsmFI GGGAC 1 cut(s) 1051
BsnI GGCC 3 cut(s) 603, 765, 882
BsoBI CYCGRG 1 cut(s) 1070
Bsp119I TTCGAA 1 cut(s) 482
Bsp1286I GDGCHC 4 cut(s) 446, 739, 778, 1233
Bsp143I GATC 4 cut(s) 102, 362, 616, 945
BspACI CCGC 3 cut(s) 211, 677, 804
BspANI GGCC 3 cut(s) 603, 765, 882
BspCNI CTCAG 4 cut(s) 304, 361, 547, 723
BspDI ATCGAT 1 cut(s) 948
BspFNI CGCG 1 cut(s) 804
BspLI GGNNCC 5 cut(s) 183, 202, 445, 1066, 1067
BspMI ACCTGC 1 cut(s) 101
BspPI GGATC 2 cut(s) 370, 953
BspT104I TTCGAA 1 cut(s) 482
BspT107I GGYRCC 1 cut(s) 200
BsrDI GCAATG 1 cut(s) 670
BsrFI RCCGGY 2 cut(s) 233, 1166
BsrI ACTGG 4 cut(s) 202, 204, 500, 1124
BssAI RCCGGY 2 cut(s) 233, 1166
BssECI CCNNGG 1 cut(s) 883
BssMI GATC 4 cut(s) 102, 362, 616, 945
BssT1I CCWWGG 1 cut(s) 883
Bst2UI CCWGG 2 cut(s) 1077, 1200
Bst4CI ACNGT 2 cut(s) 59, 878
Bst6I CTCTTC 1 cut(s) 29
BstBAI YACGTR 1 cut(s) 607
BstBI TTCGAA 1 cut(s) 482
BstC8I GCNNGC 3 cut(s) 144, 235, 802
BstDEI CTNAG 6 cut(s) 172, 312, 369, 555, 705, 731
BstFNI CGCG 1 cut(s) 804
BstH2I RGCGCY 1 cut(s) 319
BstHHI GCGC 1 cut(s) 318
BstKTI GATC 4 cut(s) 105, 365, 619, 948
BstMBI GATC 4 cut(s) 102, 362, 616, 945
BstMWI GCNNNNNNNGC 1 cut(s) 315
BstNI CCWGG 2 cut(s) 1077, 1200
BstNSI RCATGY 1 cut(s) 896
BstSCI CCNGG 2 cut(s) 1075, 1198
BstSLI GKGCMC 2 cut(s) 739, 778
BstUI CGCG 1 cut(s) 804
BstV1I GCAGC 1 cut(s) 666
BstV2I GAAGAC 1 cut(s) 292
BstX2I RGATCY 1 cut(s) 362
BstYI RGATCY 1 cut(s) 362
Bsu15I ATCGAT 1 cut(s) 948
BsuRI GGCC 3 cut(s) 603, 765, 882
BsuTUI ATCGAT 1 cut(s) 948
BtgZI GCGATG 1 cut(s) 20
BtsI GCAGTG 1 cut(s) 585
BtsIMutI CAGTG 2 cut(s) 195, 585
BveI ACCTGC 1 cut(s) 101
Cac8I GCNNGC 3 cut(s) 144, 235, 802
CaiI CAGNNNCTG 1 cut(s) 506
CfoI GCGC 1 cut(s) 318
Cfr10I RCCGGY 2 cut(s) 233, 1166
Cfr13I GGNCC 3 cut(s) 929, 1065, 1109
ClaI ATCGAT 1 cut(s) 948
CsiI ACCWGGT 1 cut(s) 1198
Csp6I GTAC 4 cut(s) 298, 695, 866, 1042
CspAI ACCGGT 1 cut(s) 1166
CviAII CATG 9 cut(s) 230, 454, 532, 632, 893, 1012, 1028, 1157, 1216
CviQI GTAC 4 cut(s) 298, 695, 866, 1042
DdeI CTNAG 6 cut(s) 172, 312, 369, 555, 705, 731
DpnI GATC 4 cut(s) 104, 364, 618, 947
DpnII GATC 4 cut(s) 102, 362, 616, 945
DraI TTTAAA 1 cut(s) 909
EaeI YGGCCR 1 cut(s) 601
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
Eco130I CCWWGG 1 cut(s) 883
Eco24I GRGCYC 1 cut(s) 446
Eco47I GGWCC 3 cut(s) 929, 1065, 1109
Eco88I CYCGRG 1 cut(s) 1070
EcoO109I RGGNCCY 2 cut(s) 1065, 1109
EcoRII CCWGG 2 cut(s) 1075, 1198
EcoT14I CCWWGG 1 cut(s) 883
EcoT22I ATGCAT 1 cut(s) 1004
EcoT38I GRGCYC 1 cut(s) 446
ErhI CCWWGG 1 cut(s) 883
FaeI CATG 9 cut(s) 233, 457, 535, 635, 896, 1015, 1031, 1160, 1219
FalI AAGNNNNNCTT 2 cut(s) 254, 286
FaqI GGGAC 1 cut(s) 1051
FatI CATG 9 cut(s) 229, 453, 531, 631, 892, 1011, 1027, 1156, 1215
FauI CCCGC 2 cut(s) 218, 797
FblI GTMKAC 1 cut(s) 157
Fnu4HI GCNGC 1 cut(s) 680
FriOI GRGCYC 1 cut(s) 446
Fsp4HI GCNGC 1 cut(s) 680
FspBI CTAG 3 cut(s) 66, 135, 1113
GlaI GCGC 1 cut(s) 317
GluI GCNGC 1 cut(s) 680
GsaI CCCAGC 1 cut(s) 729
GsuI CTGGAG 1 cut(s) 1107
HaeII RGCGCY 1 cut(s) 319
HaeIII GGCC 3 cut(s) 603, 765, 882
HapII CCGG 3 cut(s) 234, 360, 1167
HhaI GCGC 1 cut(s) 318
Hin1II CATG 9 cut(s) 233, 457, 535, 635, 896, 1015, 1031, 1160, 1219
Hin6I GCGC 1 cut(s) 316
HinP1I GCGC 1 cut(s) 316
HincII GTYRAC 2 cut(s) 158, 1177
HindII GTYRAC 2 cut(s) 158, 1177
HindIII AAGCTT 3 cut(s) 707, 970, 1100
HinfI GANTC 7 cut(s) 46, 176, 373, 406, 1051, 1188, 1193
HpaII CCGG 3 cut(s) 234, 360, 1167
HphI GGTGA 4 cut(s) 112, 489, 560, 1188
Hpy166II GTNNAC 5 cut(s) 158, 344, 718, 776, 1177
Hpy188I TCNGA 3 cut(s) 107, 313, 370
Hpy188III TCNNGA 2 cut(s) 653, 976
Hpy8I GTNNAC 5 cut(s) 158, 344, 718, 776, 1177
Hpy99I CGWCG 2 cut(s) 962, 965
HpyAV CCTTC 2 cut(s) 199, 638
HpyCH4III ACNGT 2 cut(s) 59, 878
HpyCH4IV ACGT 2 cut(s) 606, 963
HpyCH4V TGCA 8 cut(s) 10, 385, 663, 682, 776, 892, 1002, 1085
HpyF10VI GCNNNNNNNGC 1 cut(s) 315
HpyF3I CTNAG 6 cut(s) 172, 312, 369, 555, 705, 731
HpySE526I ACGT 2 cut(s) 606, 963
Hsp92II CATG 9 cut(s) 233, 457, 535, 635, 896, 1015, 1031, 1160, 1219
HspAI GCGC 1 cut(s) 316
KflI GGGWCCC 1 cut(s) 1065
KroI GCCGGC 1 cut(s) 233
KroNI GCCGGC 1 cut(s) 235
Kzo9I GATC 4 cut(s) 102, 362, 616, 945
LmnI GCTCC 3 cut(s) 107, 187, 449
Lsp1109I GCAGC 1 cut(s) 666
LweI GCATC 3 cut(s) 38, 246, 541
MabI ACCWGGT 1 cut(s) 1198
MaeI CTAG 3 cut(s) 66, 135, 1113
MaeII ACGT 2 cut(s) 606, 963
MaeIII GTNAC 3 cut(s) 895, 1007, 1194
MalI GATC 4 cut(s) 104, 364, 618, 947
MboI GATC 4 cut(s) 102, 362, 616, 945
MboII GAAGA 4 cut(s) 46, 297, 357, 565
MfeI CAATTG 2 cut(s) 620, 1221
MflI RGATCY 1 cut(s) 362
MhlI GDGCHC 4 cut(s) 446, 739, 778, 1233
MlsI TGGCCA 1 cut(s) 603
MluCI AATT 9 cut(s) 52, 253, 336, 348, 470, 559, 620, 1221, 1243
MluNI TGGCCA 1 cut(s) 603
MlyI GAGTC 2 cut(s) 170, 1202
MnlI CCTC 9 cut(s) 146, 258, 275, 336, 433, 776, 865, 1079, 1141
Mox20I TGGCCA 1 cut(s) 603
Mph1103I ATGCAT 1 cut(s) 1004
MroNI GCCGGC 1 cut(s) 233
MroXI GAANNNNTTC 1 cut(s) 225
MscI TGGCCA 1 cut(s) 603
MseI TTAA 4 cut(s) 149, 392, 854, 908
Msp20I TGGCCA 1 cut(s) 603
MspA1I CMGCKG 1 cut(s) 679
MspI CCGG 3 cut(s) 234, 360, 1167
MspR9I CCNGG 2 cut(s) 1077, 1200
MunI CAATTG 2 cut(s) 620, 1221
MvaI CCWGG 2 cut(s) 1077, 1200
MvnI CGCG 1 cut(s) 804
MwoI GCNNNNNNNGC 1 cut(s) 315
NaeI GCCGGC 1 cut(s) 235
NdeII GATC 4 cut(s) 102, 362, 616, 945
NgoMIV GCCGGC 1 cut(s) 233
NlaIII CATG 9 cut(s) 233, 457, 535, 635, 896, 1015, 1031, 1160, 1219
NlaIV GGNNCC 5 cut(s) 183, 202, 445, 1066, 1067
NmeAIII GCCGAG 2 cut(s) 158, 1252
NmuCI GTSAC 2 cut(s) 1007, 1194
NsiI ATGCAT 1 cut(s) 1004
NspI RCATGY 1 cut(s) 896
NspV TTCGAA 1 cut(s) 482
PaeR7I CTCGAG 1 cut(s) 1070
PaqCI CACCTGC 1 cut(s) 101
PcsI WCGNNNNNNNCGW 1 cut(s) 954
PdiI GCCGGC 1 cut(s) 235
PdmI GAANNNNTTC 1 cut(s) 225
PfeI GAWTC 5 cut(s) 46, 373, 406, 1051, 1188
PflMI CCANNNNNTGG 1 cut(s) 506
PinAI ACCGGT 1 cut(s) 1166
PkrI GCNGC 1 cut(s) 681
PleI GAGTC 2 cut(s) 170, 1201
PpsI GAGTC 2 cut(s) 170, 1201
Ppu21I YACGTR 1 cut(s) 607
PpuMI RGGWCCY 2 cut(s) 1065, 1109
Psp5II RGGWCCY 2 cut(s) 1065, 1109
Psp6I CCWGG 2 cut(s) 1075, 1198
PspFI CCCAGC 1 cut(s) 725
PspGI CCWGG 2 cut(s) 1075, 1198
PspN4I GGNNCC 5 cut(s) 183, 202, 445, 1066, 1067
PspPI GGNCC 3 cut(s) 929, 1065, 1109
PspPPI RGGWCCY 2 cut(s) 1065, 1109
PspXI VCTCGAGB 1 cut(s) 1070
PstNI CAGNNNCTG 1 cut(s) 506
PsuI RGATCY 1 cut(s) 362
RsaI GTAC 4 cut(s) 299, 696, 867, 1043
RsaNI GTAC 4 cut(s) 298, 695, 866, 1042
SalI GTCGAC 1 cut(s) 156
SaqAI TTAA 4 cut(s) 149, 392, 854, 908
SatI GCNGC 1 cut(s) 680
Sau3AI GATC 4 cut(s) 102, 362, 616, 945
Sau96I GGNCC 3 cut(s) 929, 1065, 1109
ScaI AGTACT 1 cut(s) 1043
SchI GAGTC 2 cut(s) 170, 1202
ScrFI CCNGG 2 cut(s) 1077, 1200
SduI GDGCHC 4 cut(s) 446, 739, 778, 1233
SexAI ACCWGGT 1 cut(s) 1198
SfaNI GCATC 3 cut(s) 38, 246, 541
Sfr274I CTCGAG 1 cut(s) 1070
SfuI TTCGAA 1 cut(s) 482
SinI GGWCC 3 cut(s) 929, 1065, 1109
SlaI CTCGAG 1 cut(s) 1070
SmiI ATTTAAAT 1 cut(s) 909
SmlI CTYRAG 3 cut(s) 260, 920, 1070
SmoI CTYRAG 3 cut(s) 260, 920, 1070
SpeI ACTAGT 1 cut(s) 65
Sse9I AATT 9 cut(s) 52, 253, 336, 348, 470, 559, 620, 1221, 1243
SsiI CCGC 3 cut(s) 211, 677, 804
SspI AATATT 1 cut(s) 305
SspMI CTAG 3 cut(s) 66, 135, 1113
StyD4I CCNGG 2 cut(s) 1075, 1198
StyI CCWWGG 1 cut(s) 883
SwaI ATTTAAAT 1 cut(s) 909
TaaI ACNGT 2 cut(s) 59, 878
TaiI ACGT 2 cut(s) 609, 966
TaqI TCGA 8 cut(s) 32, 157, 482, 516, 948, 957, 1071, 1191
TaqII GACCGA 1 cut(s) 1111
TasI AATT 9 cut(s) 52, 253, 336, 348, 470, 559, 620, 1221, 1243
TatI WGTACW 1 cut(s) 1041
TfiI GAWTC 5 cut(s) 46, 373, 406, 1051, 1188
Tru1I TTAA 4 cut(s) 149, 392, 854, 908
Tru9I TTAA 4 cut(s) 149, 392, 854, 908
TscAI CASTG 2 cut(s) 202, 592
TseFI GTSAC 2 cut(s) 1007, 1194
TseI GCWGC 1 cut(s) 679
Tsp45I GTSAC 2 cut(s) 1007, 1194
TspDTI ATGAA 5 cut(s) 218, 520, 620, 1028, 1145
TspRI CASTG 2 cut(s) 202, 592
Van91I CCANNNNNTGG 1 cut(s) 506
VneI GTGCAC 1 cut(s) 774
VpaK11BI GGWCC 3 cut(s) 929, 1065, 1109
XceI RCATGY 1 cut(s) 896
XcmI CCANNNNNNNNNTGG 1 cut(s) 939
XhoI CTCGAG 1 cut(s) 1070
XmiI GTMKAC 1 cut(s) 157
XmnI GAANNNNTTC 1 cut(s) 225
XspI CTAG 3 cut(s) 66, 135, 1113
ZrmI AGTACT 1 cut(s) 1043
Zsp2I ATGCAT 1 cut(s) 1004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.