Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0000971

Physical Location & Seq

Genomic Coordinates Reverse (-)

15470304 .. 15473817

Gene Structure 3514 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1100100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1275 bp

ATGCTGAAAAGCACATTGGACAGAATTGTGAGGAGTCATCGTAATGTTACTTCTAGTTGCAGTATCAGTATCAGTAGCATCAGCGGAGTTCCCAAAGAGAAGGTAGATTGCGTGGTAATAGGGGCAGGTGTGGTGGGATTGGCAGTGGCGAGAGAGCTTGCTTTAAAGGGCAGAGAGGTGTTGGTGTTAGATTCAGCTCCCACCTTCGGCACTTCCACCAGCTCCCGCAACAGCGAGGTCATCCATGCCGGCATCTACTACCCGACCCATTCCCTCAAGGCGAAGTTTTGTGTTAGGGGAAGATATTTACTGTACAAATATTGCTCTGAACACAATATTCCTCATAAACAAATTGGTAAACTCATAGTTGCAACTGGGTCTTCTGAGATTCCCAATTTGCATAATCTAATGCATCGCGGGATTCAAAATGGAGTTGATGGTTTGGTAATGATGGAGGGTTCAGAAGCCATGAGAATGGAACCTGAATTGGCATGTTTGAAAGCCTTACTATCACCGCTTTCTGGGATTGTGGATACACATTCGCTCATGCTATCTTTGGTGGGAGAAGCTGAAAATCATGGCACAACCTTCTCCTATAATACTACTGTTATTGGTGGCCATATTCAACAAAATCATCTATGCCTTCATGTTTCTGGAACCAAAACCCTTGAAAATTGGAATAGAAAGTTTCCATTGCAACCAGAGATGATACTTATTCCTAAACTCGTTGTGAACTCTGCCGGCTTAAGTGCCCCTGCCCTTGCAAAGCGATTTGATGGCCTCCGAACTGAACATATTCCTCCTTCCCGTTTGGCTCGTGGATGCTACTTCACTTTATCAAATACTACAATCTGTCCGTTCAGACACTTGATTTATCCTCTACCAGAGGATGGTGGACTTGGTGTGCATGTTACCCTGGATTTAAATGGTCAGGTCAAGTTTGGCCCTGATGTAGAATGGATTGATGGTGTTGATGATGTTTCAAGCTTCCTGAATAAGTTTGACTATTCTGTATGTACGAATCGCAAAGAGCTATTTTACCCAGAGATAAGGAAGTACTACCCAAATCTAAAGGATGGGTCTCTTGAGCCAGGCTATGCAGGGATTCGACCGAAGCTTTCAGGTCCTCGACAGTCTCCAGTTGATTTTCAGATACAGGGGGAGGATGTTCATGGGATAACTGGTCTCGTCAACCTTTTTGGAATAGAGTCGCCCGGTTTAACTTCAAGCATGGGAATTGCAGAGCACGTAGCAACGAGATTCTTCAGGGGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0003973 GO:0005488 GO:0008150 GO:0008152 GO:0016491 GO:0016614 GO:0016899 GO:0036094 GO:0043167 GO:0043168 GO:0047545 GO:0048037 GO:0050660 GO:0050662 GO:0055114 GO:0071949 GO:0097159 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DAO

Protein Analysis

425

Amino Acids

46.38

Weight (kDa)

8.3

Isoelectric Point (pI)

39.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAO	PF01266	36 - 419	3.1e-55	FAD dependent oxidoreductase
NAD_binding_8	PF13450	39 - 78	1.1e-06	NAD(P)-binding Rossmann-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000971)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G56840
fragaria_vesca	FvH4_1g29380 FvH4_1g29380 FvH4_1g29380 FvH4_1g29380 FvH4_1g29380 FvH4_1g29380 FvH4_1g29380 FvH4_1g29380
malus_domestica	MD14G1091600.v1.1 MD14G1100100.v1.1
prunus_persica	Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1
pyrus_communis	pycom14g09020
rosa_chinensis	RchiOBHm_Chr3g0495031
rosa_laevigata	RLG00000022654
rosa_multiflora	Rmu_ssc0000058.1_g000010
rosa_roxburghii	Rroxscaffold_6G00390830
rosa_rugosa	Rorug03G0269400
rosa_samantha	Rh3AG318400 Rh3BG353500 Rh3CG350400 Rh3DG352800
rosa_wichuraiana	Rw0G014470 Rw0G015070 Rw3G027760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	116
Acc36I	ACCTGC	1 cut(s)	116
AccB7I	CCANNNNNTGG	1 cut(s)	890
AccII	CGCG	1 cut(s)	417
AciI	CCGC	4 cut(s)	84, 226, 417, 515
AcoI	YGGCCR	1 cut(s)	616
AcuI	CTGAAG	1 cut(s)	1249
AfaI	GTAC	3 cut(s)	314, 1018, 1058
AfiI	CCNNNNNNNGG	3 cut(s)	206, 521, 890
AflII	CTTAAG	1 cut(s)	745
AgsI	TTSAA	6 cut(s)	425, 499, 626, 671, 984, 1227
AjnI	CCWGG	2 cut(s)	915, 1090
AjuI	GAANNNNNNNTTGG	1 cut(s)	31
AluBI	AGCT	7 cut(s)	157, 197, 222, 569, 987, 1033, 1117
AluI	AGCT	7 cut(s)	157, 197, 222, 569, 987, 1033, 1117
Alw21I	GWGCWC	1 cut(s)	1248
Alw26I	GTCTC	3 cut(s)	1086, 1140, 1190
AoxI	GGCC	3 cut(s)	616, 778, 943
Asp700I	GAANNNNTTC	1 cut(s)	795
AspS9I	GGNCC	2 cut(s)	944, 1124
AsuC2I	CCSGG	1 cut(s)	1215
AsuHPI	GGTGA	1 cut(s)	504
AvaII	GGWCC	1 cut(s)	1124
BaeGI	GKGCMC	1 cut(s)	754
BalI	TGGCCA	1 cut(s)	618
BarI	GAAGNNNNNNTAC	2 cut(s)	34, 66
BauI	CACGAG	1 cut(s)	816
BbsI	GAAGAC	1 cut(s)	372
Bbv12I	GWGCWC	1 cut(s)	1248
BccI	CCATC	6 cut(s)	431, 445, 770, 884, 959, 1070
BciT130I	CCWGG	2 cut(s)	917, 1092
BciVI	GTATCC	1 cut(s)	526
BcnI	CCSGG	1 cut(s)	1215
BcoDI	GTCTC	3 cut(s)	1086, 1140, 1190
BfaI	CTAG	1 cut(s)	54
BfrI	CTTAAG	1 cut(s)	745
BfuAI	ACCTGC	1 cut(s)	116
BfuI	GTATCC	1 cut(s)	526
BmcAI	AGTACT	1 cut(s)	1058
Bme1390I	CCNGG	3 cut(s)	917, 1092, 1215
Bme18I	GGWCC	1 cut(s)	1124
BmgT120I	GGNCC	2 cut(s)	944, 1124
BmiI	GGNNCC	2 cut(s)	480, 658
BmrFI	CCNGG	3 cut(s)	917, 1092, 1215
BmrI	ACTGGG	1 cut(s)	384
BmsI	GCATC	4 cut(s)	87, 261, 421, 812
BmuI	ACTGGG	1 cut(s)	384
BpiI	GAAGAC	1 cut(s)	372
BpmI	CTGGAG	1 cut(s)	1122
BpuEI	CTTGAG	2 cut(s)	260, 1106
BpuMI	CCSGG	1 cut(s)	1215
BsaAI	YACGTR	1 cut(s)	1249
BsaI	GGTCTC	2 cut(s)	1086, 1190
BsaJI	CCNNGG	1 cut(s)	915
BsaXI	ACNNNNNCTCC	2 cut(s)	25, 55
Bsc4I	CCNNNNNNNGG	3 cut(s)	206, 521, 890
Bse118I	RCCGGY	2 cut(s)	248, 740
Bse1I	ACTGG	3 cut(s)	379, 1139, 1186
Bse3DI	GCAATG	1 cut(s)	692
BseBI	CCWGG	2 cut(s)	917, 1092
BseDI	CCNNGG	1 cut(s)	915
BseGI	GGATG	5 cut(s)	240, 827, 895, 1081, 1171
BseLI	CCNNNNNNNGG	3 cut(s)	206, 521, 890
BseMI	GCAATG	1 cut(s)	692
BseMII	CTCAG	1 cut(s)	375
BseNI	ACTGG	3 cut(s)	379, 1139, 1186
BseRI	GAGGAG	1 cut(s)	46
BseSI	GKGCMC	1 cut(s)	754
Bsh1236I	CGCG	1 cut(s)	417
Bsh1285I	CGRYCG	1 cut(s)	1112
BshFI	GGCC	3 cut(s)	618, 780, 945
BsiEI	CGRYCG	1 cut(s)	1112
BsiHKAI	GWGCWC	1 cut(s)	1248
BsiSI	CCGG	3 cut(s)	249, 741, 1215
BslI	CCNNNNNNNGG	3 cut(s)	206, 521, 890
BsmAI	GTCTC	3 cut(s)	1086, 1140, 1190
BsnI	GGCC	3 cut(s)	618, 780, 945
Bso31I	GGTCTC	2 cut(s)	1086, 1190
Bsp1286I	GDGCHC	2 cut(s)	754, 1248
Bsp1407I	TGTACA	1 cut(s)	312
BspACI	CCGC	4 cut(s)	84, 226, 417, 515
BspANI	GGCC	3 cut(s)	618, 780, 945
BspCNI	CTCAG	1 cut(s)	376
BspFNI	CGCG	1 cut(s)	417
BspLI	GGNNCC	2 cut(s)	480, 658
BspMI	ACCTGC	1 cut(s)	116
BspTI	CTTAAG	1 cut(s)	745
BspTNI	GGTCTC	2 cut(s)	1086, 1190
BsrDI	GCAATG	1 cut(s)	692
BsrFI	RCCGGY	2 cut(s)	248, 740
BsrGI	TGTACA	1 cut(s)	312
BsrI	ACTGG	3 cut(s)	379, 1139, 1186
BssAI	RCCGGY	2 cut(s)	248, 740
BssECI	CCNNGG	1 cut(s)	915
BssSI	CACGAG	1 cut(s)	816
Bst2BI	CACGAG	1 cut(s)	816
Bst2UI	CCWGG	2 cut(s)	917, 1092
Bst4CI	ACNGT	3 cut(s)	312, 607, 1134
BstAFI	CTTAAG	1 cut(s)	745
BstAUI	TGTACA	1 cut(s)	312
BstBAI	YACGTR	1 cut(s)	1249
BstC8I	GCNNGC	3 cut(s)	159, 250, 742
BstDEI	CTNAG	1 cut(s)	384
BstF5I	GGATG	5 cut(s)	240, 827, 895, 1081, 1171
BstFNI	CGCG	1 cut(s)	417
BstMAI	GTCTC	3 cut(s)	1086, 1140, 1190
BstMCI	CGRYCG	1 cut(s)	1112
BstNI	CCWGG	2 cut(s)	917, 1092
BstNSI	RCATGY	2 cut(s)	495, 911
BstSCI	CCNGG	3 cut(s)	915, 1090, 1213
BstSLI	GKGCMC	1 cut(s)	754
BstUI	CGCG	1 cut(s)	417
BstV2I	GAAGAC	1 cut(s)	372
BstXI	CCANNNNNNTGG	1 cut(s)	475
BsuI	GTATCC	1 cut(s)	526
BsuRI	GGCC	3 cut(s)	618, 780, 945
BtgZI	GCGATG	1 cut(s)	398
BtsCI	GGATG	5 cut(s)	240, 827, 895, 1081, 1171
BtsI	GCAGTG	1 cut(s)	150
BtsIMutI	CAGTG	1 cut(s)	150
BveI	ACCTGC	1 cut(s)	116
Cac8I	GCNNGC	3 cut(s)	159, 250, 742
Cfr10I	RCCGGY	2 cut(s)	248, 740
Cfr13I	GGNCC	2 cut(s)	944, 1124
Csp6I	GTAC	3 cut(s)	313, 1017, 1057
CviAII	CATG	9 cut(s)	245, 469, 492, 547, 578, 647, 908, 1172, 1231
CviQI	GTAC	3 cut(s)	313, 1017, 1057
DdeI	CTNAG	1 cut(s)	384
DraI	TTTAAA	2 cut(s)	165, 924
EaeI	YGGCCR	1 cut(s)	616
Eco31I	GGTCTC	2 cut(s)	1086, 1190
Eco47I	GGWCC	1 cut(s)	1124
Eco57I	CTGAAG	1 cut(s)	1249
EcoO109I	RGGNCCY	1 cut(s)	1124
EcoRII	CCWGG	2 cut(s)	915, 1090
EcoT22I	ATGCAT	1 cut(s)	414
FaeI	CATG	9 cut(s)	248, 472, 495, 550, 581, 650, 911, 1175, 1234
FatI	CATG	9 cut(s)	244, 468, 491, 546, 577, 646, 907, 1171, 1230
FauI	CCCGC	2 cut(s)	233, 410
FokI	GGATG	5 cut(s)	227, 834, 902, 1088, 1178
FspBI	CTAG	1 cut(s)	54
GsuI	CTGGAG	1 cut(s)	1122
HaeIII	GGCC	3 cut(s)	618, 780, 945
HapII	CCGG	3 cut(s)	249, 741, 1215
Hin1II	CATG	9 cut(s)	248, 472, 495, 550, 581, 650, 911, 1175, 1234
HincII	GTYRAC	1 cut(s)	1192
HindII	GTYRAC	1 cut(s)	1192
HindIII	AAGCTT	2 cut(s)	985, 1115
HinfI	GANTC	8 cut(s)	34, 191, 388, 421, 1021, 1105, 1208, 1260
HpaII	CCGG	3 cut(s)	249, 741, 1215
HphI	GGTGA	1 cut(s)	504
Hpy166II	GTNNAC	4 cut(s)	359, 733, 896, 1192
Hpy188I	TCNGA	6 cut(s)	328, 385, 463, 785, 863, 1152
Hpy188III	TCNNGA	3 cut(s)	654, 991, 1085
Hpy8I	GTNNAC	4 cut(s)	359, 733, 896, 1192
HpyAV	CCTTC	5 cut(s)	94, 214, 598, 653, 813
HpyCH4III	ACNGT	3 cut(s)	312, 607, 1134
HpyCH4IV	ACGT	1 cut(s)	1248
HpyCH4V	TGCA	9 cut(s)	60, 371, 400, 412, 697, 764, 907, 1100, 1241
HpyF3I	CTNAG	1 cut(s)	384
HpySE526I	ACGT	1 cut(s)	1248
Hsp92II	CATG	9 cut(s)	248, 472, 495, 550, 581, 650, 911, 1175, 1234
KroI	GCCGGC	2 cut(s)	248, 740
KroNI	GCCGGC	2 cut(s)	250, 742
LmnI	GCTCC	2 cut(s)	202, 227
LweI	GCATC	4 cut(s)	87, 261, 421, 812
MaeI	CTAG	1 cut(s)	54
MaeII	ACGT	1 cut(s)	1248
MaeIII	GTNAC	2 cut(s)	46, 910
MboII	GAAGA	3 cut(s)	312, 372, 1255
MhlI	GDGCHC	2 cut(s)	754, 1248
MlsI	TGGCCA	1 cut(s)	618
MluCI	AATT	6 cut(s)	24, 351, 394, 485, 673, 1236
MluNI	TGGCCA	1 cut(s)	618
MlyI	GAGTC	2 cut(s)	43, 1217
Mox20I	TGGCCA	1 cut(s)	618
Mph1103I	ATGCAT	1 cut(s)	414
MroNI	GCCGGC	2 cut(s)	248, 740
MroXI	GAANNNNTTC	1 cut(s)	795
MscI	TGGCCA	1 cut(s)	618
MseI	TTAA	4 cut(s)	164, 746, 923, 1220
MslI	CAYNNNNRTG	2 cut(s)	42, 473
Msp20I	TGGCCA	1 cut(s)	618
MspA1I	CMGCKG	1 cut(s)	84
MspCI	CTTAAG	1 cut(s)	745
MspI	CCGG	3 cut(s)	249, 741, 1215
MspR9I	CCNGG	3 cut(s)	917, 1092, 1215
MvaI	CCWGG	2 cut(s)	917, 1092
MvnI	CGCG	1 cut(s)	417
NaeI	GCCGGC	2 cut(s)	250, 742
NciI	CCSGG	1 cut(s)	1215
NgoMIV	GCCGGC	2 cut(s)	248, 740
NlaIII	CATG	9 cut(s)	248, 472, 495, 550, 581, 650, 911, 1175, 1234
NlaIV	GGNNCC	2 cut(s)	480, 658
NsiI	ATGCAT	1 cut(s)	414
NspI	RCATGY	2 cut(s)	495, 911
PaqCI	CACCTGC	1 cut(s)	116
PdiI	GCCGGC	2 cut(s)	250, 742
PdmI	GAANNNNTTC	1 cut(s)	795
PfeI	GAWTC	6 cut(s)	191, 388, 421, 1021, 1105, 1260
PflMI	CCANNNNNTGG	1 cut(s)	890
PleI	GAGTC	2 cut(s)	42, 1216
PpsI	GAGTC	2 cut(s)	42, 1216
Ppu21I	YACGTR	1 cut(s)	1249
PpuMI	RGGWCCY	1 cut(s)	1124
Psp5II	RGGWCCY	1 cut(s)	1124
Psp6I	CCWGG	2 cut(s)	915, 1090
PspGI	CCWGG	2 cut(s)	915, 1090
PspN4I	GGNNCC	2 cut(s)	480, 658
PspPI	GGNCC	2 cut(s)	944, 1124
PspPPI	RGGWCCY	1 cut(s)	1124
RsaI	GTAC	3 cut(s)	314, 1018, 1058
RsaNI	GTAC	3 cut(s)	313, 1017, 1057
RseI	CAYNNNNRTG	2 cut(s)	42, 473
SaqAI	TTAA	4 cut(s)	164, 746, 923, 1220
Sau96I	GGNCC	2 cut(s)	944, 1124
ScaI	AGTACT	1 cut(s)	1058
SchI	GAGTC	2 cut(s)	43, 1217
ScrFI	CCNGG	3 cut(s)	917, 1092, 1215
SduI	GDGCHC	2 cut(s)	754, 1248
SfaNI	GCATC	4 cut(s)	87, 261, 421, 812
SinI	GGWCC	1 cut(s)	1124
SmiI	ATTTAAAT	1 cut(s)	924
SmiMI	CAYNNNNRTG	2 cut(s)	42, 473
SmlI	CTYRAG	3 cut(s)	275, 745, 1085
SmoI	CTYRAG	3 cut(s)	275, 745, 1085
Sse9I	AATT	6 cut(s)	24, 351, 394, 485, 673, 1236
SsiI	CCGC	4 cut(s)	84, 226, 417, 515
SspI	AATATT	2 cut(s)	320, 337
SspMI	CTAG	1 cut(s)	54
StyD4I	CCNGG	3 cut(s)	915, 1090, 1213
SwaI	ATTTAAAT	1 cut(s)	924
TaaI	ACNGT	3 cut(s)	312, 607, 1134
TaiI	ACGT	1 cut(s)	1251
TaqI	TCGA	2 cut(s)	1108, 1129
TaqII	GACCGA	1 cut(s)	1126
TasI	AATT	6 cut(s)	24, 351, 394, 485, 673, 1236
TatI	WGTACW	2 cut(s)	312, 1056
TfiI	GAWTC	6 cut(s)	191, 388, 421, 1021, 1105, 1260
Tru1I	TTAA	4 cut(s)	164, 746, 923, 1220
Tru9I	TTAA	4 cut(s)	164, 746, 923, 1220
TscAI	CASTG	1 cut(s)	150
TspDTI	ATGAA	2 cut(s)	635, 1160
TspGWI	ACGGA	1 cut(s)	846
TspRI	CASTG	1 cut(s)	150
Van91I	CCANNNNNTGG	1 cut(s)	890
Vha464I	CTTAAG	1 cut(s)	745
VpaK11BI	GGWCC	1 cut(s)	1124
XceI	RCATGY	2 cut(s)	495, 911
XmnI	GAANNNNTTC	1 cut(s)	795
XspI	CTAG	1 cut(s)	54
ZrmI	AGTACT	1 cut(s)	1058
Zsp2I	ATGCAT	1 cut(s)	414

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.