Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0000971

Physical Location & Seq

Genomic Coordinates Forward (+)

6634918 .. 6646746

Gene Structure 11829 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00390830.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1284 bp

ATGCTGAAAACAACACTCAAGAGATTGGAGACGAACTTGTCATGGTCGGTTTTGAAGAGTGTGTGGTGGAGTCGTAATTATTGTATGAGCGGTGGAATCCCGAGAGAGAGAGTAGAGTGCGTGGTGATAGGAGCAGGTGTAGTGGGATTATCAGTGGCTAGAGAGCTTGCTTTAAGAGGTCGACAGGTCTTAGTCTTAGACTCTGCTCCCACCTTCGGCACTGCCACCAGTTCCCGCAACAGCGAAGTTATCCATGCCGGCATCTACTACCCTCCTAATTCTCTCAAGGCAATCTTTTGTGTAAGAGGAAGAGACTTGCTGTACAAATATTGCTCTCAGCGCCAAATTCCTCACAAACAAATTGGTAAACTCATTGTTGCCACGGGATCTTCTGAGATTCAAAAGTTGCATTATTTAATGGATTGTGGGATTAAAAATGGGGTCGATGGTCTGGTAATGATGGAGGGTTCCGAAGCCATGAGAATAGAACCCGAATTAAGATGCTTGAAAGCCTTACTATCACCAGTTTCTGGGATTGTCGATACACATTCTTTGATGCTGTCTTTGGTGGGTGAAGCTGAGAATTGTGGCACAATCTTCTGCTATAACACTACTGTTATTGGTGGCCACATAGAACAAAATCAATTGTGCCTTCATATTTCTCAAACCAAACATCTTGAAATGTGCAATGGAAATTCTCCCCTGCAACCAGAGATAGTACTCATTCCTAAGTTTGTAGTGAACTCTGCTGGCTTGAGTGCCCCTGCCCTTGCTAAGCGATTTGATGGCCTCCAAATTGCAGTTATTCCCACTGCTCGTTATGCTCGTGGGTGCTACTTCACTCTATCCAATACTGGAATCTGTCCTTTCAAACATTTGATATATCCTATACCAGAGGACGGTGGCCTTGGAGTGCATGTTACTTTGGATTTAAATGGTCAGGTCAAGTTTGGTCCGAATGTGGAATGGATTGATGGAGTTGATGACATTTCAAGCTTCCTGAATAAGTTTGACTATTCCGTATGTAAGAGTCACATGAAGCATTTTTACCCTGAGATAAAGAAGTACTACCCGAATCTAAAAGATGAATCCCTCGAGCCGGGTTATGCAGGGATTAGACCGAAGCTTTCAGGTCCTAGACAGTCTCCAGTTGATTTTGTGATACAGGGAGAGGATATTCATGGGATAACTGGTCTTGTCAACCTTTTTGGAATCGAGTCACCTGGTTTAACTTCAAGCATGGCGATTGCCGAGCACATAGCCACCAAATTCTTCAGATGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0003973 GO:0005488 GO:0008150 GO:0008152 GO:0016491 GO:0016614 GO:0016899 GO:0036094 GO:0043167 GO:0043168 GO:0047545 GO:0048037 GO:0050660 GO:0050662 GO:0055114 GO:0071949 GO:0097159 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DAO

Protein Analysis

427

Amino Acids

46.96

Weight (kDa)

8.31

Isoelectric Point (pI)

40.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAO	PF01266	40 - 421	1.1e-51	FAD dependent oxidoreductase
NAD_binding_8	PF13450	42 - 80	9.5e-07	NAD(P)-binding Rossmann-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000971)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G56840
fragaria_vesca	FvH4_1g29380 FvH4_1g29380 FvH4_1g29380 FvH4_1g29380 FvH4_1g29380 FvH4_1g29380 FvH4_1g29380 FvH4_1g29380
malus_domestica	MD14G1091600.v1.1 MD14G1100100.v1.1
prunus_persica	Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1 Prupe.7G023400_v2.0.a1
pyrus_communis	pycom14g09020
rosa_chinensis	RchiOBHm_Chr3g0495031
rosa_laevigata	RLG00000022654
rosa_multiflora	Rmu_ssc0000058.1_g000010
rosa_roxburghii	Rroxscaffold_6G00390830
rosa_rugosa	Rorug03G0269400
rosa_samantha	Rh3AG318400 Rh3BG353500 Rh3CG350400 Rh3DG352800
rosa_wichuraiana	Rw0G014470 Rw0G015070 Rw3G027760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	125
AasI	GACNNNNNNGTC	1 cut(s)	37
Acc36I	ACCTGC	1 cut(s)	125
AccB7I	CCANNNNNTGG	1 cut(s)	530
AccBSI	CCGCTC	1 cut(s)	90
AccI	GTMKAC	1 cut(s)	181
AciI	CCGC	2 cut(s)	90, 235
AclWI	GGATC	1 cut(s)	394
AcoI	YGGCCR	1 cut(s)	625
AcsI	RAATTY	3 cut(s)	345, 694, 1268
AcuI	CTGAAG	1 cut(s)	1258
AfaI	GTAC	3 cut(s)	323, 720, 1067
AfiI	CCNNNNNNNGG	4 cut(s)	215, 530, 899, 1099
AgsI	TTSAA	7 cut(s)	55, 401, 508, 680, 871, 993, 1236
AjnI	CCWGG	1 cut(s)	1222
AluBI	AGCT	4 cut(s)	166, 578, 996, 1126
AluI	AGCT	4 cut(s)	166, 578, 996, 1126
Alw21I	GWGCWC	1 cut(s)	1257
Alw26I	GTCTC	3 cut(s)	23, 306, 1149
AlwI	GGATC	1 cut(s)	394
AlwNI	CAGNNNCTG	1 cut(s)	530
Ama87I	CYCGRG	2 cut(s)	100, 1094
AoxI	GGCC	3 cut(s)	625, 787, 904
ApoI	RAATTY	3 cut(s)	345, 694, 1268
AspLEI	GCGC	1 cut(s)	342
AspS9I	GGNCC	2 cut(s)	953, 1133
AsuC2I	CCSGG	1 cut(s)	1101
AsuHPI	GGTGA	4 cut(s)	136, 513, 584, 1212
AvaI	CYCGRG	2 cut(s)	100, 1094
AvaII	GGWCC	2 cut(s)	953, 1133
BaeGI	GKGCMC	1 cut(s)	763
BalI	TGGCCA	1 cut(s)	627
BarI	GAAGNNNNNNTAC	2 cut(s)	1031, 1063
BauI	CACGAG	1 cut(s)	825
Bbv12I	GWGCWC	1 cut(s)	1257
BccI	CCATC	4 cut(s)	440, 454, 779, 968
BciT130I	CCWGG	1 cut(s)	1224
BcnI	CCSGG	1 cut(s)	1101
BcoDI	GTCTC	3 cut(s)	23, 306, 1149
BfaI	CTAG	2 cut(s)	159, 1137
BfoI	RGCGCY	1 cut(s)	343
BfuAI	ACCTGC	1 cut(s)	125
BlpI	GCTNAGC	1 cut(s)	774
BmcAI	AGTACT	2 cut(s)	720, 1067
Bme1390I	CCNGG	2 cut(s)	1101, 1224
Bme18I	GGWCC	2 cut(s)	953, 1133
BmeT110I	CYCGRG	2 cut(s)	100, 1094
BmgT120I	GGNCC	2 cut(s)	953, 1133
BmiI	GGNNCC	1 cut(s)	469
BmrFI	CCNGG	2 cut(s)	1101, 1224
BmsI	GCATC	3 cut(s)	270, 491, 546
BpmI	CTGGAG	1 cut(s)	1131
Bpu1102I	GCTNAGC	1 cut(s)	774
BpuEI	CTTGAG	2 cut(s)	269, 775
BpuMI	CCSGG	1 cut(s)	1101
BsaJI	CCNNGG	2 cut(s)	381, 907
BsaXI	ACNNNNNCTCC	4 cut(s)	123, 153, 903, 933
Bsc4I	CCNNNNNNNGG	4 cut(s)	215, 530, 899, 1099
Bse118I	RCCGGY	1 cut(s)	257
Bse1I	ACTGG	5 cut(s)	228, 524, 859, 1148, 1195
Bse3DI	GCAATG	1 cut(s)	694
BseBI	CCWGG	1 cut(s)	1224
BseDI	CCNNGG	2 cut(s)	381, 907
BseLI	CCNNNNNNNGG	4 cut(s)	215, 530, 899, 1099
BseMI	GCAATG	1 cut(s)	694
BseMII	CTCAG	4 cut(s)	350, 384, 570, 1044
BseNI	ACTGG	5 cut(s)	228, 524, 859, 1148, 1195
BseSI	GKGCMC	1 cut(s)	763
BshFI	GGCC	3 cut(s)	627, 789, 906
BsiHKAI	GWGCWC	1 cut(s)	1257
BsiHKCI	CYCGRG	2 cut(s)	100, 1094
BsiSI	CCGG	2 cut(s)	258, 1100
BslI	CCNNNNNNNGG	4 cut(s)	215, 530, 899, 1099
BsmAI	GTCTC	3 cut(s)	23, 306, 1149
BsmBI	CGTCTC	1 cut(s)	23
BsnI	GGCC	3 cut(s)	627, 789, 906
BsoBI	CYCGRG	2 cut(s)	100, 1094
Bsp1286I	GDGCHC	2 cut(s)	763, 1257
Bsp1407I	TGTACA	1 cut(s)	321
Bsp143I	GATC	1 cut(s)	386
Bsp1720I	GCTNAGC	1 cut(s)	774
BspACI	CCGC	2 cut(s)	90, 235
BspANI	GGCC	3 cut(s)	627, 789, 906
BspCNI	CTCAG	4 cut(s)	349, 385, 571, 1045
BspLI	GGNNCC	1 cut(s)	469
BspMI	ACCTGC	1 cut(s)	125
BspPI	GGATC	1 cut(s)	394
BsrBI	CCGCTC	1 cut(s)	90
BsrDI	GCAATG	1 cut(s)	694
BsrFI	RCCGGY	1 cut(s)	257
BsrGI	TGTACA	1 cut(s)	321
BsrI	ACTGG	5 cut(s)	228, 524, 859, 1148, 1195
BssAI	RCCGGY	1 cut(s)	257
BssECI	CCNNGG	2 cut(s)	381, 907
BssMI	GATC	1 cut(s)	386
BssSI	CACGAG	1 cut(s)	825
BssT1I	CCWWGG	1 cut(s)	907
Bst2BI	CACGAG	1 cut(s)	825
Bst2UI	CCWGG	1 cut(s)	1224
Bst4CI	ACNGT	3 cut(s)	616, 902, 1143
Bst6I	CTCTTC	2 cut(s)	50, 304
BstAUI	TGTACA	1 cut(s)	321
BstC8I	GCNNGC	3 cut(s)	168, 259, 751
BstDEI	CTNAG	8 cut(s)	190, 196, 336, 393, 579, 729, 774, 1053
BstDSI	CCRYGG	1 cut(s)	381
BstH2I	RGCGCY	1 cut(s)	343
BstHHI	GCGC	1 cut(s)	342
BstKTI	GATC	1 cut(s)	389
BstMAI	GTCTC	3 cut(s)	23, 306, 1149
BstMBI	GATC	1 cut(s)	386
BstMWI	GCNNNNNNNGC	2 cut(s)	339, 821
BstNI	CCWGG	1 cut(s)	1224
BstNSI	RCATGY	1 cut(s)	920
BstSCI	CCNGG	2 cut(s)	1099, 1222
BstSLI	GKGCMC	1 cut(s)	763
BstX2I	RGATCY	1 cut(s)	386
BstYI	RGATCY	1 cut(s)	386
BsuRI	GGCC	3 cut(s)	627, 789, 906
BtgI	CCRYGG	1 cut(s)	381
BtsI	GCAGTG	2 cut(s)	219, 810
BtsIMutI	CAGTG	3 cut(s)	159, 219, 810
BveI	ACCTGC	1 cut(s)	125
Cac8I	GCNNGC	3 cut(s)	168, 259, 751
CaiI	CAGNNNCTG	1 cut(s)	530
CfoI	GCGC	1 cut(s)	342
Cfr10I	RCCGGY	1 cut(s)	257
Cfr13I	GGNCC	2 cut(s)	953, 1133
CsiI	ACCWGGT	1 cut(s)	1222
Csp6I	GTAC	3 cut(s)	322, 719, 1066
CviAII	CATG	7 cut(s)	42, 254, 478, 917, 1036, 1181, 1240
CviQI	GTAC	3 cut(s)	322, 719, 1066
DdeI	CTNAG	8 cut(s)	190, 196, 336, 393, 579, 729, 774, 1053
DpnI	GATC	1 cut(s)	388
DpnII	GATC	1 cut(s)	386
DraI	TTTAAA	1 cut(s)	933
DrdI	GACNNNNNNGTC	1 cut(s)	37
DseDI	GACNNNNNNGTC	1 cut(s)	37
EaeI	YGGCCR	1 cut(s)	625
Eam1104I	CTCTTC	2 cut(s)	50, 304
EarI	CTCTTC	2 cut(s)	50, 304
Eco130I	CCWWGG	1 cut(s)	907
Eco47I	GGWCC	2 cut(s)	953, 1133
Eco57I	CTGAAG	1 cut(s)	1258
Eco88I	CYCGRG	2 cut(s)	100, 1094
EcoO109I	RGGNCCY	1 cut(s)	1133
EcoRII	CCWGG	1 cut(s)	1222
EcoT14I	CCWWGG	1 cut(s)	907
ErhI	CCWWGG	1 cut(s)	907
Esp3I	CGTCTC	1 cut(s)	23
FaeI	CATG	7 cut(s)	45, 257, 481, 920, 1039, 1184, 1243
FalI	AAGNNNNNCTT	2 cut(s)	278, 310
FatI	CATG	7 cut(s)	41, 253, 477, 916, 1035, 1180, 1239
FauI	CCCGC	1 cut(s)	242
FblI	GTMKAC	1 cut(s)	181
FspBI	CTAG	2 cut(s)	159, 1137
GlaI	GCGC	1 cut(s)	341
GsuI	CTGGAG	1 cut(s)	1131
HaeII	RGCGCY	1 cut(s)	343
HaeIII	GGCC	3 cut(s)	627, 789, 906
HapII	CCGG	2 cut(s)	258, 1100
HhaI	GCGC	1 cut(s)	342
Hin1II	CATG	7 cut(s)	45, 257, 481, 920, 1039, 1184, 1243
Hin6I	GCGC	1 cut(s)	340
HinP1I	GCGC	1 cut(s)	340
HincII	GTYRAC	2 cut(s)	182, 1201
HindII	GTYRAC	2 cut(s)	182, 1201
HindIII	AAGCTT	2 cut(s)	994, 1124
HpaII	CCGG	2 cut(s)	258, 1100
HphI	GGTGA	4 cut(s)	136, 513, 584, 1212
Hpy166II	GTNNAC	4 cut(s)	182, 368, 742, 1201
Hpy188I	TCNGA	4 cut(s)	394, 472, 957, 1277
Hpy188III	TCNNGA	4 cut(s)	19, 100, 677, 1000
Hpy8I	GTNNAC	4 cut(s)	182, 368, 742, 1201
HpyAV	CCTTC	2 cut(s)	223, 662
HpyCH4III	ACNGT	3 cut(s)	616, 902, 1143
HpyCH4V	TGCA	6 cut(s)	409, 687, 706, 800, 916, 1109
HpyF10VI	GCNNNNNNNGC	2 cut(s)	339, 821
HpyF3I	CTNAG	8 cut(s)	190, 196, 336, 393, 579, 729, 774, 1053
Hsp92II	CATG	7 cut(s)	45, 257, 481, 920, 1039, 1184, 1243
HspAI	GCGC	1 cut(s)	340
KroI	GCCGGC	1 cut(s)	257
KroNI	GCCGGC	1 cut(s)	259
Kzo9I	GATC	1 cut(s)	386
LmnI	GCTCC	2 cut(s)	131, 211
LweI	GCATC	3 cut(s)	270, 491, 546
MabI	ACCWGGT	1 cut(s)	1222
MaeI	CTAG	2 cut(s)	159, 1137
MaeIII	GTNAC	3 cut(s)	919, 1031, 1218
MalI	GATC	1 cut(s)	388
MbiI	CCGCTC	1 cut(s)	90
MboI	GATC	1 cut(s)	386
MboII	GAAGA	5 cut(s)	67, 321, 381, 589, 1264
MfeI	CAATTG	1 cut(s)	644
MflI	RGATCY	1 cut(s)	386
MhlI	GDGCHC	2 cut(s)	763, 1257
MlsI	TGGCCA	1 cut(s)	627
MluNI	TGGCCA	1 cut(s)	627
MlyI	GAGTC	4 cut(s)	79, 194, 1039, 1226
MnlI	CCTC	9 cut(s)	170, 282, 299, 360, 457, 800, 889, 1103, 1165
Mox20I	TGGCCA	1 cut(s)	627
MroNI	GCCGGC	1 cut(s)	257
MscI	TGGCCA	1 cut(s)	627
MseI	TTAA	7 cut(s)	173, 416, 432, 497, 932, 1229, 1282
Msp20I	TGGCCA	1 cut(s)	627
MspI	CCGG	2 cut(s)	258, 1100
MspR9I	CCNGG	2 cut(s)	1101, 1224
MunI	CAATTG	1 cut(s)	644
MvaI	CCWGG	1 cut(s)	1224
MwoI	GCNNNNNNNGC	2 cut(s)	339, 821
NaeI	GCCGGC	1 cut(s)	259
NciI	CCSGG	1 cut(s)	1101
NdeII	GATC	1 cut(s)	386
NgoMIV	GCCGGC	1 cut(s)	257
NlaIII	CATG	7 cut(s)	45, 257, 481, 920, 1039, 1184, 1243
NlaIV	GGNNCC	1 cut(s)	469
NmeAIII	GCCGAG	1 cut(s)	1276
NmuCI	GTSAC	2 cut(s)	1031, 1218
NspI	RCATGY	1 cut(s)	920
PaeR7I	CTCGAG	1 cut(s)	1094
PaqCI	CACCTGC	1 cut(s)	125
PcsI	WCGNNNNNNNCGW	1 cut(s)	823
PdiI	GCCGGC	1 cut(s)	259
PfeI	GAWTC	6 cut(s)	96, 397, 858, 1075, 1088, 1212
PflMI	CCANNNNNTGG	1 cut(s)	530
PleI	GAGTC	4 cut(s)	78, 194, 1038, 1225
PpsI	GAGTC	4 cut(s)	78, 194, 1038, 1225
PpuMI	RGGWCCY	1 cut(s)	1133
Psp5II	RGGWCCY	1 cut(s)	1133
Psp6I	CCWGG	1 cut(s)	1222
PspGI	CCWGG	1 cut(s)	1222
PspN4I	GGNNCC	1 cut(s)	469
PspPI	GGNCC	2 cut(s)	953, 1133
PspPPI	RGGWCCY	1 cut(s)	1133
PspXI	VCTCGAGB	1 cut(s)	1094
PstNI	CAGNNNCTG	1 cut(s)	530
PsuI	RGATCY	1 cut(s)	386
RsaI	GTAC	3 cut(s)	323, 720, 1067
RsaNI	GTAC	3 cut(s)	322, 719, 1066
SalI	GTCGAC	1 cut(s)	180
SaqAI	TTAA	7 cut(s)	173, 416, 432, 497, 932, 1229, 1282
Sau3AI	GATC	1 cut(s)	386
Sau96I	GGNCC	2 cut(s)	953, 1133
ScaI	AGTACT	2 cut(s)	720, 1067
SchI	GAGTC	4 cut(s)	79, 194, 1039, 1226
ScrFI	CCNGG	2 cut(s)	1101, 1224
SduI	GDGCHC	2 cut(s)	763, 1257
SexAI	ACCWGGT	1 cut(s)	1222
SfaNI	GCATC	3 cut(s)	270, 491, 546
Sfr274I	CTCGAG	1 cut(s)	1094
SinI	GGWCC	2 cut(s)	953, 1133
SlaI	CTCGAG	1 cut(s)	1094
SmiI	ATTTAAAT	1 cut(s)	933
SmlI	CTYRAG	4 cut(s)	17, 284, 754, 1094
SmoI	CTYRAG	4 cut(s)	17, 284, 754, 1094
SsiI	CCGC	2 cut(s)	90, 235
SspI	AATATT	1 cut(s)	329
SspMI	CTAG	2 cut(s)	159, 1137
StyD4I	CCNGG	2 cut(s)	1099, 1222
StyI	CCWWGG	1 cut(s)	907
SwaI	ATTTAAAT	1 cut(s)	933
TaaI	ACNGT	3 cut(s)	616, 902, 1143
TaqI	TCGA	5 cut(s)	181, 444, 540, 1095, 1215
TaqII	GACCGA	1 cut(s)	1135
TatI	WGTACW	3 cut(s)	321, 718, 1065
TfiI	GAWTC	6 cut(s)	96, 397, 858, 1075, 1088, 1212
Tru1I	TTAA	7 cut(s)	173, 416, 432, 497, 932, 1229, 1282
Tru9I	TTAA	7 cut(s)	173, 416, 432, 497, 932, 1229, 1282
TscAI	CASTG	3 cut(s)	159, 226, 817
TseFI	GTSAC	2 cut(s)	1031, 1218
Tsp45I	GTSAC	2 cut(s)	1031, 1218
TspDTI	ATGAA	4 cut(s)	644, 1052, 1101, 1169
TspGWI	ACGGA	1 cut(s)	1009
TspRI	CASTG	3 cut(s)	159, 226, 817
Van91I	CCANNNNNTGG	1 cut(s)	530
VpaK11BI	GGWCC	2 cut(s)	953, 1133
XapI	RAATTY	3 cut(s)	345, 694, 1268
XceI	RCATGY	1 cut(s)	920
XhoI	CTCGAG	1 cut(s)	1094
XmiI	GTMKAC	1 cut(s)	181
XspI	CTAG	2 cut(s)	159, 1137
ZrmI	AGTACT	2 cut(s)	720, 1067

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.