Prupe.7G023400_v2.0.a1

L-2-hydroxyglutarate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0000971
Physical Location & Seq
Reverse (-)
3664491 .. 3672942
8452 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G023400.18

Sequence Viewer

Length: 1305 bp
ATGCTGAAAAGCAAAATCCAAAGCCTGGAAAGGATTTCATCGTCGTCGTCAGTTGTCTTCAAGATTTTGAAGAGTCGTAATATTGCTACTACTACTGGTAGTACCAGCAGCAGTGGAGTTCCAAAAGAGAAGGTAGATTGCGTGGTGATAGGGGCAGGTGTGGTGGGATTAGCAGTAGCGAGAGAGCTTACTTTAAAGGGCAGAGAGGTGTTGGTGTTAGAATCAGCTTCCACCTTCGGCACTGGCATAAGTTCCCGCAACAGCGAGGTCATCCATGCCGGCATCTACTATCCTCCCAATTCTCTCAAGGCAATCTTATGTGTAAGAGGAAGAGAAATGCTTTACAAATATTGCTCTGAACACAATATCCCTCATAACCAAATTGGTAAACTTATAGTAGCAACAGGATCTTCAGAGATTCCAAAGTTGCATAATCTAATGAATAACGGGATAAAAAATGGGGTTGGTGGTTTGGTTATGATGGAAGGTTCAGAAGCTACGAGGATGGAACCAGAATTGCGATGCTTGAAAGCCTTACTATCACCAGTTTCTGGGATTGTTGATACACATTCCTTGATGCTGTCTTTGGTGGGTGAAGCTGAAAATCATGGGGCAACCTTCTCCTATAATACTACAGTTATTGGTGGTCATATTGAAGAAAATCGCCTAAGCCTTCATATTTCTGAAACCAAACACCTTGAAAATTGGAATGGAAAATTTCCATTGCAACCAGAGATGGTACTCATTCCTAAGCTTGTAGTAAACTCTGCTGGCTTGAGTGCCCCTGTCCTTGCAAAGCGATTTGATGGCCTTCGTAGTGCAGTTATTCCTCCTACCTATTATGCTCGCGGGTGCTACTTCACGCTATCAAATACTACAATATGTCCTTTCAAACATTTGATTTATCCTATACCAGAGGATGGTGGCCTTGGTGTGCATGTTACTCTGGATTTGAATGGTCAGGTCAAGTTTGGCCCCAATGTAGAATGGATTGATGGTATTGATGACGTTTCAAGCTTCCTAAACAAGTTTGACTATTCTGTATGTACGAATCGTGCGAAGCTATTTTACCCAGAGATAAGGAAGTACTACCCAAATCTAAAAGATGGGTCTCTCGATCCAGGTTATGCAGGGATTCGACCAAAGCTTTCAGGTCCTCGACAGTCTCCAGTTGATTTTGTGATACAGGGCGAGGACATTCATGGGATAACTGGCCTTGTTAACCTTTTTGGTATTGAGTCACCTGGTTTAACTTCAAGCATGGGAGTTGCAGAACACATAGCAACCAGATTCTTCAGATGTTAA

Protein Analysis

435

Amino Acids

47.12

Weight (kDa)

8.5

Isoelectric Point (pI)

40.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 146
Acc36I ACCTGC 1 cut(s) 146
AccB7I CCANNNNNTGG 3 cut(s) 25, 551, 920
AccII CGCG 1 cut(s) 849
AciI CCGC 2 cut(s) 256, 849
AclWI GGATC 2 cut(s) 415, 1112
AcsI RAATTY 1 cut(s) 716
AcuI CTGAAG 2 cut(s) 396, 1279
AfaI GTAC 4 cut(s) 103, 741, 1048, 1088
AfiI CCNNNNNNNGG 3 cut(s) 25, 551, 920
AgsI TTSAA 9 cut(s) 61, 70, 529, 656, 701, 892, 955, 1014, 1257
AjnI CCWGG 3 cut(s) 24, 1120, 1243
AloI GAACNNNNNNTCC 2 cut(s) 351, 383
AluBI AGCT 8 cut(s) 187, 227, 497, 599, 754, 1017, 1063, 1147
AluI AGCT 8 cut(s) 187, 227, 497, 599, 754, 1017, 1063, 1147
Alw26I GTCTC 2 cut(s) 1116, 1170
AlwI GGATC 2 cut(s) 415, 1112
AlwNI CAGNNNCTG 1 cut(s) 551
AoxI GGCC 4 cut(s) 808, 925, 973, 1213
ApeKI GCWGC 1 cut(s) 108
ApoI RAATTY 1 cut(s) 716
AspS9I GGNCC 2 cut(s) 974, 1154
AsuHPI GGTGA 4 cut(s) 157, 534, 605, 1233
AvaII GGWCC 1 cut(s) 1154
BaeGI GKGCMC 1 cut(s) 784
BarI GAAGNNNNNNTAC 2 cut(s) 1052, 1084
BbsI GAAGAC 1 cut(s) 49
BbvI GCAGC 1 cut(s) 120
BccI CCATC 7 cut(s) 475, 499, 730, 800, 914, 989, 1100
BciT130I CCWGG 3 cut(s) 26, 1122, 1245
BcoDI GTCTC 2 cut(s) 1116, 1170
BfmI CTRYAG 1 cut(s) 633
BfuAI ACCTGC 1 cut(s) 146
BisI GCNGC 1 cut(s) 109
BlsI GCNGC 1 cut(s) 110
BmcAI AGTACT 1 cut(s) 1088
Bme1390I CCNGG 3 cut(s) 26, 1122, 1245
Bme18I GGWCC 1 cut(s) 1154
BmgT120I GGNCC 2 cut(s) 974, 1154
BmiI GGNNCC 2 cut(s) 510, 976
BmrFI CCNGG 3 cut(s) 26, 1122, 1245
BmsI GCATC 3 cut(s) 291, 512, 567
BpiI GAAGAC 1 cut(s) 49
BpmI CTGGAG 1 cut(s) 1152
Bpu10I CCTNAGC 2 cut(s) 668, 750
BpuEI CTTGAG 2 cut(s) 290, 796
BsaI GGTCTC 1 cut(s) 1116
BsaJI CCNNGG 1 cut(s) 928
Bsc4I CCNNNNNNNGG 3 cut(s) 25, 551, 920
Bse118I RCCGGY 1 cut(s) 278
Bse1I ACTGG 5 cut(s) 100, 247, 545, 1169, 1216
Bse3DI GCAATG 1 cut(s) 722
BseBI CCWGG 3 cut(s) 26, 1122, 1245
BseDI CCNNGG 1 cut(s) 928
BseGI GGATG 3 cut(s) 270, 510, 925
BseLI CCNNNNNNNGG 3 cut(s) 25, 551, 920
BseMI GCAATG 1 cut(s) 722
BseNI ACTGG 5 cut(s) 100, 247, 545, 1169, 1216
BseSI GKGCMC 1 cut(s) 784
BseXI GCAGC 1 cut(s) 120
BsgI GTGCAG 1 cut(s) 840
Bsh1236I CGCG 1 cut(s) 849
BshFI GGCC 4 cut(s) 810, 927, 975, 1215
BsiSI CCGG 1 cut(s) 279
BslI CCNNNNNNNGG 3 cut(s) 25, 551, 920
BsmAI GTCTC 2 cut(s) 1116, 1170
BsnI GGCC 4 cut(s) 810, 927, 975, 1215
Bso31I GGTCTC 1 cut(s) 1116
Bsp1286I GDGCHC 1 cut(s) 784
Bsp143I GATC 2 cut(s) 407, 1117
BspACI CCGC 2 cut(s) 256, 849
BspANI GGCC 4 cut(s) 810, 927, 975, 1215
BspFNI CGCG 1 cut(s) 849
BspLI GGNNCC 2 cut(s) 510, 976
BspMI ACCTGC 1 cut(s) 146
BspPI GGATC 2 cut(s) 415, 1112
BspTNI GGTCTC 1 cut(s) 1116
BsrDI GCAATG 1 cut(s) 722
BsrFI RCCGGY 1 cut(s) 278
BsrI ACTGG 5 cut(s) 100, 247, 545, 1169, 1216
BssAI RCCGGY 1 cut(s) 278
BssECI CCNNGG 1 cut(s) 928
BssMI GATC 2 cut(s) 407, 1117
BssT1I CCWWGG 1 cut(s) 928
Bst2UI CCWGG 3 cut(s) 26, 1122, 1245
Bst4CI ACNGT 2 cut(s) 637, 1164
Bst6I CTCTTC 2 cut(s) 65, 325
BstC8I GCNNGC 3 cut(s) 280, 772, 847
BstDEI CTNAG 2 cut(s) 668, 750
BstF5I GGATG 3 cut(s) 270, 510, 925
BstFNI CGCG 1 cut(s) 849
BstKTI GATC 2 cut(s) 410, 1120
BstMAI GTCTC 2 cut(s) 1116, 1170
BstMBI GATC 2 cut(s) 407, 1117
BstNI CCWGG 3 cut(s) 26, 1122, 1245
BstNSI RCATGY 1 cut(s) 941
BstSCI CCNGG 3 cut(s) 24, 1120, 1243
BstSFI CTRYAG 1 cut(s) 633
BstSLI GKGCMC 1 cut(s) 784
BstUI CGCG 1 cut(s) 849
BstV1I GCAGC 1 cut(s) 120
BstV2I GAAGAC 1 cut(s) 49
BstX2I RGATCY 1 cut(s) 407
BstYI RGATCY 1 cut(s) 407
BsuRI GGCC 4 cut(s) 810, 927, 975, 1215
BtgZI GCGATG 1 cut(s) 535
BtsCI GGATG 3 cut(s) 270, 510, 925
BtsI GCAGTG 1 cut(s) 118
BtsIMutI CAGTG 2 cut(s) 118, 240
BveI ACCTGC 1 cut(s) 146
Cac8I GCNNGC 3 cut(s) 280, 772, 847
CaiI CAGNNNCTG 1 cut(s) 551
Cfr10I RCCGGY 1 cut(s) 278
Cfr13I GGNCC 2 cut(s) 974, 1154
CsiI ACCWGGT 1 cut(s) 1243
Csp6I GTAC 4 cut(s) 102, 740, 1047, 1087
CviAII CATG 5 cut(s) 275, 608, 938, 1202, 1261
CviQI GTAC 4 cut(s) 102, 740, 1047, 1087
DdeI CTNAG 2 cut(s) 668, 750
DpnI GATC 2 cut(s) 409, 1119
DpnII GATC 2 cut(s) 407, 1117
DraI TTTAAA 1 cut(s) 195
Eam1104I CTCTTC 2 cut(s) 65, 325
EarI CTCTTC 2 cut(s) 65, 325
Eco130I CCWWGG 1 cut(s) 928
Eco31I GGTCTC 1 cut(s) 1116
Eco47I GGWCC 1 cut(s) 1154
Eco57I CTGAAG 2 cut(s) 396, 1279
EcoO109I RGGNCCY 1 cut(s) 1154
EcoRII CCWGG 3 cut(s) 24, 1120, 1243
EcoT14I CCWWGG 1 cut(s) 928
ErhI CCWWGG 1 cut(s) 928
FaeI CATG 5 cut(s) 278, 611, 941, 1205, 1264
FalI AAGNNNNNCTT 2 cut(s) 299, 331
FatI CATG 5 cut(s) 274, 607, 937, 1201, 1260
FauI CCCGC 2 cut(s) 263, 842
Fnu4HI GCNGC 1 cut(s) 109
FokI GGATG 3 cut(s) 257, 517, 932
Fsp4HI GCNGC 1 cut(s) 109
GluI GCNGC 1 cut(s) 109
GsuI CTGGAG 1 cut(s) 1152
HaeIII GGCC 4 cut(s) 810, 927, 975, 1215
HapII CCGG 1 cut(s) 279
Hin1II CATG 5 cut(s) 278, 611, 941, 1205, 1264
HincII GTYRAC 1 cut(s) 1222
HindII GTYRAC 1 cut(s) 1222
HindIII AAGCTT 3 cut(s) 752, 1015, 1145
HinfI GANTC 7 cut(s) 73, 221, 418, 1051, 1135, 1238, 1290
HpaI GTTAAC 1 cut(s) 1222
HpaII CCGG 1 cut(s) 279
HphI GGTGA 4 cut(s) 157, 534, 605, 1233
Hpy166II GTNNAC 3 cut(s) 389, 763, 1222
Hpy188I TCNGA 5 cut(s) 358, 415, 493, 685, 1298
Hpy188III TCNNGA 3 cut(s) 61, 947, 1115
Hpy8I GTNNAC 3 cut(s) 389, 763, 1222
Hpy99I CGWCG 2 cut(s) 46, 49
HpyAV CCTTC 6 cut(s) 124, 244, 479, 628, 683, 821
HpyCH4III ACNGT 2 cut(s) 637, 1164
HpyCH4IV ACGT 1 cut(s) 1008
HpyCH4V TGCA 7 cut(s) 430, 727, 794, 821, 937, 1130, 1271
HpyF3I CTNAG 2 cut(s) 668, 750
HpySE526I ACGT 1 cut(s) 1008
Hsp92II CATG 5 cut(s) 278, 611, 941, 1205, 1264
KroI GCCGGC 1 cut(s) 278
KroNI GCCGGC 1 cut(s) 280
KspAI GTTAAC 1 cut(s) 1222
Kzo9I GATC 2 cut(s) 407, 1117
Lsp1109I GCAGC 1 cut(s) 120
LweI GCATC 3 cut(s) 291, 512, 567
MabI ACCWGGT 1 cut(s) 1243
MaeII ACGT 1 cut(s) 1008
MaeIII GTNAC 2 cut(s) 940, 1239
MalI GATC 2 cut(s) 409, 1119
MboI GATC 2 cut(s) 407, 1117
MboII GAAGA 6 cut(s) 49, 82, 342, 402, 668, 1285
MflI RGATCY 1 cut(s) 407
MhlI GDGCHC 1 cut(s) 784
MluCI AATT 5 cut(s) 298, 381, 515, 703, 716
MlyI GAGTC 2 cut(s) 82, 1247
MroNI GCCGGC 1 cut(s) 278
MseI TTAA 4 cut(s) 194, 1221, 1250, 1303
MspI CCGG 1 cut(s) 279
MspR9I CCNGG 3 cut(s) 26, 1122, 1245
MvaI CCWGG 3 cut(s) 26, 1122, 1245
MvnI CGCG 1 cut(s) 849
NaeI GCCGGC 1 cut(s) 280
NdeII GATC 2 cut(s) 407, 1117
NgoMIV GCCGGC 1 cut(s) 278
NlaIII CATG 5 cut(s) 278, 611, 941, 1205, 1264
NlaIV GGNNCC 2 cut(s) 510, 976
NmuCI GTSAC 1 cut(s) 1239
NspI RCATGY 1 cut(s) 941
PaqCI CACCTGC 1 cut(s) 146
PcsI WCGNNNNNNNCGW 1 cut(s) 1055
PdiI GCCGGC 1 cut(s) 280
PfeI GAWTC 5 cut(s) 221, 418, 1051, 1135, 1290
PflMI CCANNNNNTGG 3 cut(s) 25, 551, 920
PkrI GCNGC 1 cut(s) 110
PleI GAGTC 2 cut(s) 81, 1246
PpsI GAGTC 2 cut(s) 81, 1246
PpuMI RGGWCCY 1 cut(s) 1154
Psp5II RGGWCCY 1 cut(s) 1154
Psp6I CCWGG 3 cut(s) 24, 1120, 1243
PspGI CCWGG 3 cut(s) 24, 1120, 1243
PspN4I GGNNCC 2 cut(s) 510, 976
PspPI GGNCC 2 cut(s) 974, 1154
PspPPI RGGWCCY 1 cut(s) 1154
PstNI CAGNNNCTG 1 cut(s) 551
PsuI RGATCY 1 cut(s) 407
RsaI GTAC 4 cut(s) 103, 741, 1048, 1088
RsaNI GTAC 4 cut(s) 102, 740, 1047, 1087
SaqAI TTAA 4 cut(s) 194, 1221, 1250, 1303
SatI GCNGC 1 cut(s) 109
Sau3AI GATC 2 cut(s) 407, 1117
Sau96I GGNCC 2 cut(s) 974, 1154
ScaI AGTACT 1 cut(s) 1088
SchI GAGTC 2 cut(s) 82, 1247
ScrFI CCNGG 3 cut(s) 26, 1122, 1245
SduI GDGCHC 1 cut(s) 784
SexAI ACCWGGT 1 cut(s) 1243
SfaNI GCATC 3 cut(s) 291, 512, 567
SfcI CTRYAG 1 cut(s) 633
SinI GGWCC 1 cut(s) 1154
SmlI CTYRAG 2 cut(s) 305, 775
SmoI CTYRAG 2 cut(s) 305, 775
Sse9I AATT 5 cut(s) 298, 381, 515, 703, 716
SsiI CCGC 2 cut(s) 256, 849
SspI AATATT 2 cut(s) 82, 350
StyD4I CCNGG 3 cut(s) 24, 1120, 1243
StyI CCWWGG 1 cut(s) 928
TaaI ACNGT 2 cut(s) 637, 1164
TaiI ACGT 1 cut(s) 1011
TaqI TCGA 3 cut(s) 1116, 1138, 1159
TasI AATT 5 cut(s) 298, 381, 515, 703, 716
TatI WGTACW 1 cut(s) 1086
TfiI GAWTC 5 cut(s) 221, 418, 1051, 1135, 1290
Tru1I TTAA 4 cut(s) 194, 1221, 1250, 1303
Tru9I TTAA 4 cut(s) 194, 1221, 1250, 1303
TscAI CASTG 2 cut(s) 118, 247
TseFI GTSAC 1 cut(s) 1239
TseI GCWGC 1 cut(s) 108
Tsp45I GTSAC 1 cut(s) 1239
TspDTI ATGAA 4 cut(s) 27, 455, 665, 1190
TspRI CASTG 2 cut(s) 118, 247
Van91I CCANNNNNTGG 3 cut(s) 25, 551, 920
VpaK11BI GGWCC 1 cut(s) 1154
XapI RAATTY 1 cut(s) 716
XceI RCATGY 1 cut(s) 941
ZrmI AGTACT 1 cut(s) 1088
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.