AT3G56950

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014244
Physical Location & Seq
Reverse (-)
21078297 .. 21079925
1629 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G56950.2

Sequence Viewer

Length: 714 bp
ATGGGACGAATCGGTTTAGTAGTGACTGATCTGGTATTGTCGTTCATGTGGATATGGGCAGGAGTTCTGGTCAACATTTTAGTCCATGGAGTTCTTGGATTTAGCCGGACCGATCCTTCCGGTGAGATCGTTCGTTATCTCTTCTCTATCATCTCCATGTTCATCTTCGCCTACCTTCAACAAGCCACCAAAGGCGGGCTTTATAATCCTTTGACCGCTTTGGCTGCTGGAGTCTCCGGTGGCTTCAGCAGTTTCATCTTCTCCGTCTTCGTTCGAATCCCCGTCGAGGTAATAGGATCAATTCTTGCGGTTAAGCATATCATTCACGTATTCCCTGAGATTGGGAAAGGACCTAAACTAAATGTAGCAATTCACCACGGTGCTTTAACAGAAGGGATACTAACATTCTTCATTGTACTGCTCTCAATGGGACTAACAAGAAAAATCCCGGGAAGTTTCTTCATGAAGACTTGGATCGGTAGTCTTGCTAAGTTAACTCTTCATATTCTTGGCTCTGATCTTACCGGTGGATGCATGAACCCTGCAGCTGTTATGGGATGGGCTTATGCACGCGGTGAACATATAACGAAAGAGCATTTACTTGTGTATTGGCTTGGACCTGTGAAGGCTACGTTGCTTGCAGTTTGGTTCTTCAAAGTTGTGTTTAAGCCTCTCACCGAAGAACAAGAGAAACCTAAGGCCAAATCTGAGTGA

Protein Analysis

237

Amino Acids

25.94

Weight (kDa)

9.58

Isoelectric Point (pI)

30.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 9 - 217 2e-07 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014244)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56950 AT3G56950
fragaria_vesca FvH4_1g24870
malus_domestica MD02G1144000.v1.1 MD15G1257400.v1.1
prunus_persica Prupe.7G020600_v2.0.a1
pyrus_communis pycom02g11390 pycom15g22520
rosa_chinensis RchiOBHm_Chr2g0122531
rosa_laevigata RLG00000018682
rosa_roxburghii Rroxscaffold_2G00121500
rosa_rugosa Rorug02G0239300
rosa_samantha Rh2AG298000 Rh2BG306700 Rh2CG284800 Rh2DG321600
rosa_wichuraiana Rw2G023690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 204
AccII CGCG 1 cut(s) 573
AciI CCGC 4 cut(s) 195, 216, 308, 573
AclWI GGATC 3 cut(s) 107, 304, 482
AcuI CTGAAG 1 cut(s) 229
AdeI CACNNNGTG 1 cut(s) 575
AfaI GTAC 1 cut(s) 417
AfiI CCNNNNNNNGG 3 cut(s) 195, 286, 341
AgeI ACCGGT 1 cut(s) 524
AgsI TTSAA 2 cut(s) 179, 655
AleI CACNNNNGTG 1 cut(s) 378
AluBI AGCT 1 cut(s) 548
AluI AGCT 1 cut(s) 548
Alw26I GTCTC 1 cut(s) 238
AlwI GGATC 3 cut(s) 107, 304, 482
Ama87I CYCGRG 1 cut(s) 448
AoxI GGCC 1 cut(s) 699
ApeKI GCWGC 2 cut(s) 224, 545
AsiGI ACCGGT 1 cut(s) 524
AspS9I GGNCC 3 cut(s) 108, 350, 617
AsuC2I CCSGG 2 cut(s) 449, 450
AsuHPI GGTGA 4 cut(s) 134, 365, 587, 667
AsuII TTCGAA 1 cut(s) 274
AvaI CYCGRG 1 cut(s) 448
AvaII GGWCC 3 cut(s) 108, 350, 617
AxyI CCTNAGG 1 cut(s) 696
BbsI GAAGAC 2 cut(s) 259, 473
BbvI GCAGC 2 cut(s) 211, 557
BccI CCATC 1 cut(s) 552
BciVI GTATCC 1 cut(s) 390
BcnI CCSGG 2 cut(s) 449, 450
BcoDI GTCTC 1 cut(s) 238
BfmI CTRYAG 1 cut(s) 543
BfuI GTATCC 1 cut(s) 390
BisI GCNGC 2 cut(s) 225, 546
BlsI GCNGC 2 cut(s) 226, 547
Bme1390I CCNGG 2 cut(s) 449, 450
Bme18I GGWCC 3 cut(s) 108, 350, 617
BmeT110I CYCGRG 1 cut(s) 448
BmgT120I GGNCC 3 cut(s) 108, 350, 617
BmrFI CCNGG 2 cut(s) 449, 450
BmsI GCATC 1 cut(s) 521
BpiI GAAGAC 2 cut(s) 259, 473
BpmI CTGGAG 1 cut(s) 249
Bpu14I TTCGAA 1 cut(s) 274
BpuMI CCSGG 2 cut(s) 449, 450
BsaAI YACGTR 1 cut(s) 328
BsaJI CCNNGG 3 cut(s) 85, 376, 448
BsaWI WCCGGW 3 cut(s) 119, 236, 524
BsaXI ACNNNNNCTCC 2 cut(s) 54, 84
Bsc4I CCNNNNNNNGG 3 cut(s) 195, 286, 341
Bse118I RCCGGY 1 cut(s) 524
Bse21I CCTNAGG 1 cut(s) 696
BseDI CCNNGG 3 cut(s) 85, 376, 448
BseGI GGATG 2 cut(s) 536, 563
BseLI CCNNNNNNNGG 3 cut(s) 195, 286, 341
BseMII CTCAG 2 cut(s) 327, 699
BseXI GCAGC 2 cut(s) 211, 557
Bsh1236I CGCG 1 cut(s) 573
BshFI GGCC 1 cut(s) 701
BshTI ACCGGT 1 cut(s) 524
BsiHKCI CYCGRG 1 cut(s) 448
BsiSI CCGG 5 cut(s) 106, 120, 237, 449, 525
BslFI GGGAC 2 cut(s) 18, 444
BslI CCNNNNNNNGG 3 cut(s) 195, 286, 341
BsmAI GTCTC 1 cut(s) 238
BsmFI GGGAC 2 cut(s) 18, 444
BsnI GGCC 1 cut(s) 701
BsoBI CYCGRG 1 cut(s) 448
Bsp119I TTCGAA 1 cut(s) 274
Bsp143I GATC 6 cut(s) 28, 112, 126, 296, 474, 517
Bsp19I CCATGG 1 cut(s) 85
BspACI CCGC 4 cut(s) 195, 216, 308, 573
BspANI GGCC 1 cut(s) 701
BspCNI CTCAG 2 cut(s) 328, 700
BspFNI CGCG 1 cut(s) 573
BspHI TCATGA 1 cut(s) 462
BspMAI CTGCAG 1 cut(s) 547
BspPI GGATC 3 cut(s) 107, 304, 482
BspT104I TTCGAA 1 cut(s) 274
BsrFI RCCGGY 1 cut(s) 524
BssAI RCCGGY 1 cut(s) 524
BssECI CCNNGG 3 cut(s) 85, 376, 448
BssMI GATC 6 cut(s) 28, 112, 126, 296, 474, 517
BssT1I CCWWGG 1 cut(s) 85
Bst4CI ACNGT 1 cut(s) 380
Bst6I CTCTTC 2 cut(s) 146, 504
BstBAI YACGTR 1 cut(s) 328
BstBI TTCGAA 1 cut(s) 274
BstC8I GCNNGC 3 cut(s) 197, 571, 639
BstDEI CTNAG 4 cut(s) 336, 489, 696, 708
BstDSI CCRYGG 2 cut(s) 85, 376
BstF5I GGATG 2 cut(s) 536, 563
BstFNI CGCG 1 cut(s) 573
BstKTI GATC 6 cut(s) 31, 115, 129, 299, 477, 520
BstMAI GTCTC 1 cut(s) 238
BstMBI GATC 6 cut(s) 28, 112, 126, 296, 474, 517
BstMWI GCNNNNNNNGC 1 cut(s) 224
BstSCI CCNGG 2 cut(s) 447, 448
BstSFI CTRYAG 1 cut(s) 543
BstUI CGCG 1 cut(s) 573
BstV1I GCAGC 2 cut(s) 211, 557
BstV2I GAAGAC 2 cut(s) 259, 473
Bsu36I CCTNAGG 1 cut(s) 696
BsuI GTATCC 1 cut(s) 390
BsuRI GGCC 1 cut(s) 701
BtgI CCRYGG 2 cut(s) 85, 376
BtsCI GGATG 2 cut(s) 536, 563
Cac8I GCNNGC 3 cut(s) 197, 571, 639
CciI TCATGA 1 cut(s) 462
Cfr10I RCCGGY 1 cut(s) 524
Cfr13I GGNCC 3 cut(s) 108, 350, 617
Cfr9I CCCGGG 1 cut(s) 448
CpoI CGGWCCG 1 cut(s) 108
Csp6I GTAC 1 cut(s) 416
CspAI ACCGGT 1 cut(s) 524
CspI CGGWCCG 1 cut(s) 108
CviAII CATG 5 cut(s) 46, 86, 157, 463, 535
CviQI GTAC 1 cut(s) 416
DdeI CTNAG 4 cut(s) 336, 489, 696, 708
DpnI GATC 6 cut(s) 30, 114, 128, 298, 476, 519
DpnII GATC 6 cut(s) 28, 112, 126, 296, 474, 517
DraIII CACNNNGTG 1 cut(s) 575
Eam1104I CTCTTC 2 cut(s) 146, 504
EarI CTCTTC 2 cut(s) 146, 504
Eco130I CCWWGG 1 cut(s) 85
Eco47I GGWCC 3 cut(s) 108, 350, 617
Eco57I CTGAAG 1 cut(s) 229
Eco81I CCTNAGG 1 cut(s) 696
Eco88I CYCGRG 1 cut(s) 448
EcoO109I RGGNCCY 1 cut(s) 350
EcoT14I CCWWGG 1 cut(s) 85
EcoT22I ATGCAT 1 cut(s) 536
ErhI CCWWGG 1 cut(s) 85
FaeI CATG 5 cut(s) 49, 89, 160, 466, 538
FalI AAGNNNNNCTT 2 cut(s) 183, 215
FaqI GGGAC 2 cut(s) 18, 444
FatI CATG 5 cut(s) 45, 85, 156, 462, 534
FauI CCCGC 1 cut(s) 188
Fnu4HI GCNGC 2 cut(s) 225, 546
FokI GGATG 2 cut(s) 543, 570
Fsp4HI GCNGC 2 cut(s) 225, 546
GluI GCNGC 2 cut(s) 225, 546
GsuI CTGGAG 1 cut(s) 249
HaeIII GGCC 1 cut(s) 701
HapII CCGG 5 cut(s) 106, 120, 237, 449, 525
Hin1II CATG 5 cut(s) 49, 89, 160, 466, 538
HincII GTYRAC 2 cut(s) 73, 495
HindII GTYRAC 2 cut(s) 73, 495
HinfI GANTC 3 cut(s) 9, 231, 276
HpaI GTTAAC 1 cut(s) 495
HpaII CCGG 5 cut(s) 106, 120, 237, 449, 525
HphI GGTGA 4 cut(s) 134, 365, 587, 667
Hpy166II GTNNAC 3 cut(s) 73, 495, 578
Hpy188I TCNGA 2 cut(s) 517, 709
Hpy188III TCNNGA 1 cut(s) 463
Hpy8I GTNNAC 3 cut(s) 73, 495, 578
Hpy99I CGWCG 1 cut(s) 287
HpyAV CCTTC 4 cut(s) 126, 185, 386, 619
HpyCH4III ACNGT 1 cut(s) 380
HpyCH4IV ACGT 2 cut(s) 327, 632
HpyCH4V TGCA 4 cut(s) 534, 545, 569, 641
HpyF10VI GCNNNNNNNGC 1 cut(s) 224
HpyF3I CTNAG 4 cut(s) 336, 489, 696, 708
HpySE526I ACGT 2 cut(s) 327, 632
Hsp92II CATG 5 cut(s) 49, 89, 160, 466, 538
KspAI GTTAAC 1 cut(s) 495
Kzo9I GATC 6 cut(s) 28, 112, 126, 296, 474, 517
Lsp1109I GCAGC 2 cut(s) 211, 557
LweI GCATC 1 cut(s) 521
MaeII ACGT 2 cut(s) 327, 632
MaeIII GTNAC 1 cut(s) 22
MalI GATC 6 cut(s) 30, 114, 128, 298, 476, 519
MboI GATC 6 cut(s) 28, 112, 126, 296, 474, 517
MluCI AATT 2 cut(s) 300, 369
MlyI GAGTC 1 cut(s) 240
MnlI CCTC 2 cut(s) 280, 681
Mph1103I ATGCAT 1 cut(s) 536
MseI TTAA 4 cut(s) 312, 386, 494, 666
MslI CAYNNNNRTG 2 cut(s) 155, 378
MspA1I CMGCKG 1 cut(s) 548
MspI CCGG 5 cut(s) 106, 120, 237, 449, 525
MspR9I CCNGG 2 cut(s) 449, 450
MvnI CGCG 1 cut(s) 573
MwoI GCNNNNNNNGC 1 cut(s) 224
NciI CCSGG 2 cut(s) 449, 450
NcoI CCATGG 1 cut(s) 85
NdeII GATC 6 cut(s) 28, 112, 126, 296, 474, 517
NlaIII CATG 5 cut(s) 49, 89, 160, 466, 538
NmuCI GTSAC 1 cut(s) 22
NsiI ATGCAT 1 cut(s) 536
NspV TTCGAA 1 cut(s) 274
OliI CACNNNNGTG 1 cut(s) 378
PagI TCATGA 1 cut(s) 462
PfeI GAWTC 2 cut(s) 9, 276
PinAI ACCGGT 1 cut(s) 524
PkrI GCNGC 2 cut(s) 226, 547
PleI GAGTC 1 cut(s) 239
PpsI GAGTC 1 cut(s) 239
Ppu21I YACGTR 1 cut(s) 328
PpuMI RGGWCCY 1 cut(s) 350
PsiI TTATAA 1 cut(s) 204
Psp5II RGGWCCY 1 cut(s) 350
PspPI GGNCC 3 cut(s) 108, 350, 617
PspPPI RGGWCCY 1 cut(s) 350
PstI CTGCAG 1 cut(s) 547
PvuII CAGCTG 1 cut(s) 548
RsaI GTAC 1 cut(s) 417
RsaNI GTAC 1 cut(s) 416
RseI CAYNNNNRTG 2 cut(s) 155, 378
Rsr2I CGGWCCG 1 cut(s) 108
RsrII CGGWCCG 1 cut(s) 108
SaqAI TTAA 4 cut(s) 312, 386, 494, 666
SatI GCNGC 2 cut(s) 225, 546
Sau3AI GATC 6 cut(s) 28, 112, 126, 296, 474, 517
Sau96I GGNCC 3 cut(s) 108, 350, 617
SchI GAGTC 1 cut(s) 240
ScrFI CCNGG 2 cut(s) 449, 450
SetI ASST 8 cut(s) 177, 291, 330, 355, 550, 622, 635, 697
SfaNI GCATC 1 cut(s) 521
SfcI CTRYAG 1 cut(s) 543
SfuI TTCGAA 1 cut(s) 274
SinI GGWCC 3 cut(s) 108, 350, 617
SmaI CCCGGG 1 cut(s) 450
SmiMI CAYNNNNRTG 2 cut(s) 155, 378
Sse9I AATT 2 cut(s) 300, 369
SsiI CCGC 4 cut(s) 195, 216, 308, 573
StyD4I CCNGG 2 cut(s) 447, 448
StyI CCWWGG 1 cut(s) 85
TaaI ACNGT 1 cut(s) 380
TaiI ACGT 2 cut(s) 330, 635
TaqI TCGA 2 cut(s) 274, 285
TaqII GACCGA 1 cut(s) 125
TasI AATT 2 cut(s) 300, 369
TatI WGTACW 1 cut(s) 415
TfiI GAWTC 2 cut(s) 9, 276
Tru1I TTAA 4 cut(s) 312, 386, 494, 666
Tru9I TTAA 4 cut(s) 312, 386, 494, 666
TseFI GTSAC 1 cut(s) 22
TseI GCWGC 2 cut(s) 224, 545
Tsp45I GTSAC 1 cut(s) 22
TspDTI ATGAA 8 cut(s) 34, 151, 244, 400, 451, 479, 491, 551
TspGWI ACGGA 1 cut(s) 253
TspMI CCCGGG 1 cut(s) 448
VpaK11BI GGWCC 3 cut(s) 108, 350, 617
XcmI CCANNNNNNNNNTGG 1 cut(s) 92
XmaI CCCGGG 1 cut(s) 448
Zsp2I ATGCAT 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.