RLG00000018682

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014244
Physical Location & Seq
Forward (+)
33883793 .. 33886748
2956 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018682

Sequence Viewer

Length: 699 bp
ATGGCGAGGGCTGCTTTGCTCGCATCGGATTTGGTAATCTCATTCATGTGGGTCTTGTCAGGAGTTTTGATCAAGAAATTTATCCACAATGTTTTGGGGTTGGCTCACGAACCCAGTGGTGATATTCTCTACTTCGCCCTCTCAATCATCAACATGTTTTTCTTTGCTTTCTTGGCTAAGATTACCAAGGGTGGCACCTACAATCCTCTCACCGTTTTCTCTGACGCAATCACCGGCGATTTCAGCCGATTCCTTTTTACTGTTGCTGTCAGAATCCCTGCTCAGGTTATTGGCTCTGTTGCCGGGGTTAAGCTCATAATGGAGATCTTTCCTGGAATAGGTCTTGGGCCACGTTTGACTGTTGACATCCATCGAGGGGCCCTTACAGAAGGATTGCTGACATTTGCAATTGTTACTATCTCACTTGGTCTTTACAGAAAAATCCCGGGGAGCTTTTTCATGAAGACATGGATCTCAAGTGTTTCCAAGTTAACTCTTCATATTCTTGGCTCCGATCTGACTGGTGGTTGCATGAACCCAGCTTCTGTGATGGGATGGGCTTATGCTCGCGGAGACCATATAACCAAGGAACATATTCTTGTTTATTGGCTTGCCCCAATAGAGGCAACTCTGCTGGCAGTATGGACATTTAGGTTGCTATTTCCACCAAAAGAGGACAAGAAAGCGAAAGCAGAATGA

Protein Analysis

233

Amino Acids

25.4

Weight (kDa)

9.49

Isoelectric Point (pI)

26.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 20 - 216 9.1e-08 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0014244)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56950 AT3G56950
fragaria_vesca FvH4_1g24870
malus_domestica MD02G1144000.v1.1 MD15G1257400.v1.1
prunus_persica Prupe.7G020600_v2.0.a1
pyrus_communis pycom02g11390 pycom15g22520
rosa_chinensis RchiOBHm_Chr2g0122531
rosa_laevigata RLG00000018682
rosa_roxburghii Rroxscaffold_2G00121500
rosa_rugosa Rorug02G0239300
rosa_samantha Rh2AG298000 Rh2BG306700 Rh2CG284800 Rh2DG321600
rosa_wichuraiana Rw2G023690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 194
AccII CGCG 1 cut(s) 570
AciI CCGC 1 cut(s) 570
AclWI GGATC 1 cut(s) 479
AcsI RAATTY 1 cut(s) 77
AfiI CCNNNNNNNGG 4 cut(s) 283, 338, 376, 622
AflIII ACRYGT 1 cut(s) 153
AjnI CCWGG 1 cut(s) 331
AluBI AGCT 3 cut(s) 313, 453, 542
AluI AGCT 3 cut(s) 313, 453, 542
Alw26I GTCTC 1 cut(s) 567
AlwI GGATC 1 cut(s) 479
AlwNI CAGNNNCTG 1 cut(s) 545
Ama87I CYCGRG 1 cut(s) 445
AoxI GGCC 2 cut(s) 347, 378
ApaI GGGCCC 1 cut(s) 382
ApeKI GCWGC 1 cut(s) 11
ApoI RAATTY 1 cut(s) 77
Asp700I GAANNNNTTC 1 cut(s) 594
AspS9I GGNCC 3 cut(s) 347, 378, 379
AsuC2I CCSGG 3 cut(s) 304, 446, 447
AsuHPI GGTGA 3 cut(s) 131, 202, 223
AvaI CYCGRG 1 cut(s) 445
BaeGI GKGCMC 1 cut(s) 382
BanI GGYRCC 1 cut(s) 194
BanII GRGCYC 1 cut(s) 382
BbsI GAAGAC 1 cut(s) 470
BccI CCATC 3 cut(s) 378, 544, 549
BciT130I CCWGG 1 cut(s) 333
BclI TGATCA 1 cut(s) 69
BcnI CCSGG 3 cut(s) 304, 446, 447
BcoDI GTCTC 1 cut(s) 567
BglII AGATCT 1 cut(s) 324
BisI GCNGC 1 cut(s) 12
BlsI GCNGC 1 cut(s) 13
Bme1390I CCNGG 4 cut(s) 304, 333, 446, 447
BmeT110I CYCGRG 1 cut(s) 445
BmgT120I GGNCC 3 cut(s) 347, 378, 379
BmiI GGNNCC 4 cut(s) 196, 379, 380, 511
BmrFI CCNGG 4 cut(s) 304, 333, 446, 447
BmrI ACTGGG 1 cut(s) 108
BmsI GCATC 1 cut(s) 32
BmuI ACTGGG 1 cut(s) 108
BpiI GAAGAC 1 cut(s) 470
Bpu10I CCTNAGC 1 cut(s) 282
BpuEI CTTGAG 1 cut(s) 460
BpuMI CCSGG 3 cut(s) 304, 446, 447
BsaI GGTCTC 1 cut(s) 567
BsaJI CCNNGG 5 cut(s) 186, 303, 445, 446, 585
Bsc4I CCNNNNNNNGG 4 cut(s) 283, 338, 376, 622
Bse118I RCCGGY 1 cut(s) 233
Bse1I ACTGG 2 cut(s) 114, 526
BseBI CCWGG 1 cut(s) 333
BseDI CCNNGG 5 cut(s) 186, 303, 445, 446, 585
BseGI GGATG 2 cut(s) 366, 560
BseLI CCNNNNNNNGG 4 cut(s) 283, 338, 376, 622
BseMII CTCAG 1 cut(s) 296
BseNI ACTGG 2 cut(s) 114, 526
BseSI GKGCMC 1 cut(s) 382
BseYI CCCAGC 1 cut(s) 538
Bsh1236I CGCG 1 cut(s) 570
BshFI GGCC 2 cut(s) 349, 380
BshNI GGYRCC 1 cut(s) 194
BsiHKCI CYCGRG 1 cut(s) 445
BsiSI CCGG 3 cut(s) 234, 303, 446
BslI CCNNNNNNNGG 4 cut(s) 283, 338, 376, 622
BsmAI GTCTC 1 cut(s) 567
BsnI GGCC 2 cut(s) 349, 380
Bso31I GGTCTC 1 cut(s) 567
BsoBI CYCGRG 1 cut(s) 445
Bsp120I GGGCCC 1 cut(s) 378
Bsp1286I GDGCHC 1 cut(s) 382
Bsp143I GATC 4 cut(s) 69, 324, 471, 514
BspACI CCGC 1 cut(s) 570
BspANI GGCC 2 cut(s) 349, 380
BspCNI CTCAG 1 cut(s) 295
BspFNI CGCG 1 cut(s) 570
BspHI TCATGA 1 cut(s) 459
BspLI GGNNCC 4 cut(s) 196, 379, 380, 511
BspPI GGATC 1 cut(s) 479
BspT107I GGYRCC 1 cut(s) 194
BspTNI GGTCTC 1 cut(s) 567
BsrFI RCCGGY 1 cut(s) 233
BsrI ACTGG 2 cut(s) 114, 526
BssAI RCCGGY 1 cut(s) 233
BssECI CCNNGG 5 cut(s) 186, 303, 445, 446, 585
BssMI GATC 4 cut(s) 69, 324, 471, 514
BssT1I CCWWGG 2 cut(s) 186, 585
Bst2UI CCWGG 1 cut(s) 333
Bst4CI ACNGT 3 cut(s) 214, 262, 361
Bst6I CTCTTC 1 cut(s) 501
BstC8I GCNNGC 4 cut(s) 21, 568, 612, 636
BstDEI CTNAG 2 cut(s) 177, 282
BstENI CCTNNNNNAGG 1 cut(s) 336
BstF5I GGATG 2 cut(s) 366, 560
BstFNI CGCG 1 cut(s) 570
BstKTI GATC 4 cut(s) 72, 327, 474, 517
BstMAI GTCTC 1 cut(s) 567
BstMBI GATC 4 cut(s) 69, 324, 471, 514
BstMWI GCNNNNNNNGC 4 cut(s) 11, 20, 173, 243
BstNI CCWGG 1 cut(s) 333
BstNSI RCATGY 1 cut(s) 157
BstSCI CCNGG 4 cut(s) 302, 331, 444, 445
BstSLI GKGCMC 1 cut(s) 382
BstUI CGCG 1 cut(s) 570
BstV2I GAAGAC 1 cut(s) 470
BstX2I RGATCY 2 cut(s) 324, 471
BstYI RGATCY 2 cut(s) 324, 471
BsuRI GGCC 2 cut(s) 349, 380
BtsCI GGATG 2 cut(s) 366, 560
BtsIMutI CAGTG 1 cut(s) 121
Cac8I GCNNGC 4 cut(s) 21, 568, 612, 636
CaiI CAGNNNCTG 1 cut(s) 545
CciI TCATGA 1 cut(s) 459
Cfr10I RCCGGY 1 cut(s) 233
Cfr13I GGNCC 3 cut(s) 347, 378, 379
Cfr9I CCCGGG 1 cut(s) 445
CseI GACGC 1 cut(s) 233
CviAII CATG 5 cut(s) 46, 154, 460, 468, 532
DdeI CTNAG 2 cut(s) 177, 282
DpnI GATC 4 cut(s) 71, 326, 473, 516
DpnII GATC 4 cut(s) 69, 324, 471, 514
Eam1104I CTCTTC 1 cut(s) 501
EarI CTCTTC 1 cut(s) 501
Eco130I CCWWGG 2 cut(s) 186, 585
Eco24I GRGCYC 1 cut(s) 382
Eco31I GGTCTC 1 cut(s) 567
Eco88I CYCGRG 1 cut(s) 445
EcoNI CCTNNNNNAGG 1 cut(s) 336
EcoO109I RGGNCCY 2 cut(s) 378, 379
EcoRII CCWGG 1 cut(s) 331
EcoT14I CCWWGG 2 cut(s) 186, 585
EcoT38I GRGCYC 1 cut(s) 382
ErhI CCWWGG 2 cut(s) 186, 585
FaeI CATG 5 cut(s) 49, 157, 463, 471, 535
FatI CATG 5 cut(s) 45, 153, 459, 467, 531
FbaI TGATCA 1 cut(s) 69
Fnu4HI GCNGC 1 cut(s) 12
FokI GGATG 2 cut(s) 353, 567
FriOI GRGCYC 1 cut(s) 382
Fsp4HI GCNGC 1 cut(s) 12
GluI GCNGC 1 cut(s) 12
GsaI CCCAGC 1 cut(s) 542
HaeIII GGCC 2 cut(s) 349, 380
HapII CCGG 3 cut(s) 234, 303, 446
HgaI GACGC 1 cut(s) 233
Hin1II CATG 5 cut(s) 49, 157, 463, 471, 535
HincII GTYRAC 2 cut(s) 364, 492
HindII GTYRAC 2 cut(s) 364, 492
HinfI GANTC 2 cut(s) 249, 273
HpaI GTTAAC 1 cut(s) 492
HpaII CCGG 3 cut(s) 234, 303, 446
HphI GGTGA 3 cut(s) 131, 202, 223
Hpy166II GTNNAC 2 cut(s) 364, 492
Hpy188I TCNGA 5 cut(s) 28, 223, 272, 514, 519
Hpy188III TCNNGA 4 cut(s) 60, 73, 107, 460
Hpy8I GTNNAC 2 cut(s) 364, 492
HpyAV CCTTC 1 cut(s) 383
HpyCH4III ACNGT 3 cut(s) 214, 262, 361
HpyCH4IV ACGT 1 cut(s) 352
HpyCH4V TGCA 2 cut(s) 407, 531
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 20, 173, 243
HpyF3I CTNAG 2 cut(s) 177, 282
HpySE526I ACGT 1 cut(s) 352
Hsp92II CATG 5 cut(s) 49, 157, 463, 471, 535
Ksp22I TGATCA 1 cut(s) 69
KspAI GTTAAC 1 cut(s) 492
Kzo9I GATC 4 cut(s) 69, 324, 471, 514
LmnI GCTCC 2 cut(s) 450, 515
LweI GCATC 1 cut(s) 32
MaeII ACGT 1 cut(s) 352
MaeIII GTNAC 1 cut(s) 412
MalI GATC 4 cut(s) 71, 326, 473, 516
MboI GATC 4 cut(s) 69, 324, 471, 514
MboII GAAGA 2 cut(s) 475, 488
MfeI CAATTG 1 cut(s) 408
MflI RGATCY 2 cut(s) 324, 471
MhlI GDGCHC 1 cut(s) 382
MluCI AATT 2 cut(s) 77, 408
MnlI CCTC 5 cut(s) 149, 216, 368, 616, 667
MroXI GAANNNNTTC 1 cut(s) 594
MseI TTAA 2 cut(s) 309, 491
MslI CAYNNNNRTG 2 cut(s) 46, 152
MspI CCGG 3 cut(s) 234, 303, 446
MspR9I CCNGG 4 cut(s) 304, 333, 446, 447
MunI CAATTG 1 cut(s) 408
MvaI CCWGG 1 cut(s) 333
MvnI CGCG 1 cut(s) 570
MwoI GCNNNNNNNGC 4 cut(s) 11, 20, 173, 243
NciI CCSGG 3 cut(s) 304, 446, 447
NdeII GATC 4 cut(s) 69, 324, 471, 514
NlaIII CATG 5 cut(s) 49, 157, 463, 471, 535
NlaIV GGNNCC 4 cut(s) 196, 379, 380, 511
NspI RCATGY 1 cut(s) 157
PagI TCATGA 1 cut(s) 459
PciI ACATGT 1 cut(s) 153
PdmI GAANNNNTTC 1 cut(s) 594
PfeI GAWTC 2 cut(s) 249, 273
PfoI TCCNGGA 1 cut(s) 331
PkrI GCNGC 1 cut(s) 13
PscI ACATGT 1 cut(s) 153
Psp6I CCWGG 1 cut(s) 331
PspFI CCCAGC 1 cut(s) 538
PspGI CCWGG 1 cut(s) 331
PspN4I GGNNCC 4 cut(s) 196, 379, 380, 511
PspOMI GGGCCC 1 cut(s) 378
PspPI GGNCC 3 cut(s) 347, 378, 379
PstNI CAGNNNCTG 1 cut(s) 545
PsuI RGATCY 2 cut(s) 324, 471
RseI CAYNNNNRTG 2 cut(s) 46, 152
SaqAI TTAA 2 cut(s) 309, 491
SatI GCNGC 1 cut(s) 12
Sau3AI GATC 4 cut(s) 69, 324, 471, 514
Sau96I GGNCC 3 cut(s) 347, 378, 379
ScrFI CCNGG 4 cut(s) 304, 333, 446, 447
SduI GDGCHC 1 cut(s) 382
SetI ASST 8 cut(s) 200, 288, 315, 343, 355, 455, 544, 656
SfaNI GCATC 1 cut(s) 32
SgrAI CRCCGGYG 1 cut(s) 233
SmaI CCCGGG 1 cut(s) 447
SmiMI CAYNNNNRTG 2 cut(s) 46, 152
SmlI CTYRAG 1 cut(s) 475
SmoI CTYRAG 1 cut(s) 475
Sse9I AATT 2 cut(s) 77, 408
SsiI CCGC 1 cut(s) 570
StyD4I CCNGG 4 cut(s) 302, 331, 444, 445
StyI CCWWGG 2 cut(s) 186, 585
TaaI ACNGT 3 cut(s) 214, 262, 361
TaiI ACGT 1 cut(s) 355
TaqI TCGA 1 cut(s) 373
TasI AATT 2 cut(s) 77, 408
TfiI GAWTC 2 cut(s) 249, 273
Tru1I TTAA 2 cut(s) 309, 491
Tru9I TTAA 2 cut(s) 309, 491
TscAI CASTG 1 cut(s) 121
TseI GCWGC 1 cut(s) 11
TspDTI ATGAA 5 cut(s) 34, 448, 476, 488, 548
TspMI CCCGGG 1 cut(s) 445
TspRI CASTG 1 cut(s) 121
XagI CCTNNNNNAGG 1 cut(s) 336
XapI RAATTY 1 cut(s) 77
XceI RCATGY 1 cut(s) 157
XmaI CCCGGG 1 cut(s) 445
XmnI GAANNNNTTC 1 cut(s) 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.