Rh2DG321600

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0014244
Physical Location & Seq
Forward (+)
40197796 .. 40203995
6200 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG321600.1

Sequence Viewer

Length: 735 bp
ATGGGGAGGGCTGCTTTGCTCGCATCGGATTTGGTAATCTCATTCATGTGGGTCTTGTCAGGAGTTTTAATCAAGAAATTTATTCACAATGTGTTGGGGTTGGCTCACGAACCTAGTGGTGACATCCTCTACTTCGCCCTCTCAATCATCAACATGTTTTTCTTTGCTTTCTTGGCTAAGATTACCAAGGGTGGCACCTACAATCCTCTCACCGTTTTCTCTGACGCAATCACCGGCGATTTCAGCCGATTCCTTTTCACTGTTGCTGCCAGAATCCCTGCTCAGGTTATTGGCTCTGTTGCTGGGGTCAAGCTCATAATGGAGATCTTTCCTGGAATAGGTCTCGGGCCACGTTTGACTGTTGACATCCATCGAGGGGCCCTTACAGAAGGATTGCTGACATTTGCAATCGTTACTATCTCACTTGGTCTGTACAGAAAAATCCCGGGGAGCTTTTTCATGAAGACATGGATCTCAAGTGTTTCCAAGTTAACTCTTCATATTCTTGGCTCTGATCTGACTGGTGGTTGCATGAACCCAGCTTCTATATCAAAGAACAGGAGGGTGACGGTCAGGAACTGGAACGGCAAGGTTATTGTCGACATTCGCGAGTTCTATGTCAAGGAGGGCAAGCAATTGCCTGGAAAGAAAGGAATCTCACTACCGATGGATCAGTGGAATGTTCTTCGGAGTCATGTGGACGAGATTGACAAGGTTGTTAACGAGACTGGTTAG

Protein Analysis

244

Amino Acids

26.73

Weight (kDa)

9.77

Isoelectric Point (pI)

20.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PC4 PF02229 182 - 232 5.4e-25 Transcriptional Coactivator p15 (PC4)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014244)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56950 AT3G56950
fragaria_vesca FvH4_1g24870
malus_domestica MD02G1144000.v1.1 MD15G1257400.v1.1
prunus_persica Prupe.7G020600_v2.0.a1
pyrus_communis pycom02g11390 pycom15g22520
rosa_chinensis RchiOBHm_Chr2g0122531
rosa_laevigata RLG00000018682
rosa_roxburghii Rroxscaffold_2G00121500
rosa_rugosa Rorug02G0239300
rosa_samantha Rh2AG298000 Rh2BG306700 Rh2CG284800 Rh2DG321600
rosa_wichuraiana Rw2G023690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 194
AccI GTMKAC 1 cut(s) 600
AccII CGCG 1 cut(s) 609
AclWI GGATC 2 cut(s) 479, 678
AcsI RAATTY 1 cut(s) 77
AdeI CACNNNGTG 1 cut(s) 91
AfaI GTAC 1 cut(s) 434
AfiI CCNNNNNNNGG 3 cut(s) 283, 338, 376
AflIII ACRYGT 1 cut(s) 153
AjnI CCWGG 2 cut(s) 331, 640
AluBI AGCT 3 cut(s) 313, 453, 542
AluI AGCT 3 cut(s) 313, 453, 542
Alw26I GTCTC 2 cut(s) 347, 719
AlwI GGATC 2 cut(s) 479, 678
AlwNI CAGNNNCTG 1 cut(s) 579
Ama87I CYCGRG 2 cut(s) 344, 445
AoxI GGCC 2 cut(s) 347, 378
ApaI GGGCCC 1 cut(s) 382
ApeKI GCWGC 2 cut(s) 11, 266
ApoI RAATTY 1 cut(s) 77
AspS9I GGNCC 3 cut(s) 347, 378, 379
AsuC2I CCSGG 2 cut(s) 446, 447
AsuHPI GGTGA 4 cut(s) 131, 202, 223, 577
AvaI CYCGRG 2 cut(s) 344, 445
BaeGI GKGCMC 1 cut(s) 382
BanI GGYRCC 1 cut(s) 194
BanII GRGCYC 1 cut(s) 382
BbsI GAAGAC 1 cut(s) 470
BbvI GCAGC 1 cut(s) 253
BccI CCATC 2 cut(s) 378, 661
BceAI ACGGC 1 cut(s) 601
BciT130I CCWGG 2 cut(s) 333, 642
BcnI CCSGG 2 cut(s) 446, 447
BcoDI GTCTC 2 cut(s) 347, 719
BfaI CTAG 1 cut(s) 114
BglII AGATCT 1 cut(s) 324
BisI GCNGC 2 cut(s) 12, 267
BlsI GCNGC 2 cut(s) 13, 268
Bme1390I CCNGG 4 cut(s) 333, 446, 447, 642
BmeT110I CYCGRG 2 cut(s) 344, 445
BmgT120I GGNCC 3 cut(s) 347, 378, 379
BmiI GGNNCC 3 cut(s) 196, 379, 380
BmrFI CCNGG 4 cut(s) 333, 446, 447, 642
BmsI GCATC 1 cut(s) 32
BpiI GAAGAC 1 cut(s) 470
Bpu10I CCTNAGC 1 cut(s) 282
BpuEI CTTGAG 1 cut(s) 460
BpuMI CCSGG 2 cut(s) 446, 447
BsaI GGTCTC 1 cut(s) 347
BsaJI CCNNGG 3 cut(s) 186, 445, 446
Bsc4I CCNNNNNNNGG 3 cut(s) 283, 338, 376
Bse118I RCCGGY 1 cut(s) 233
Bse1I ACTGG 3 cut(s) 526, 584, 733
BseBI CCWGG 2 cut(s) 333, 642
BseDI CCNNGG 3 cut(s) 186, 445, 446
BseGI GGATG 2 cut(s) 123, 366
BseLI CCNNNNNNNGG 3 cut(s) 283, 338, 376
BseMII CTCAG 1 cut(s) 296
BseNI ACTGG 3 cut(s) 526, 584, 733
BseSI GKGCMC 1 cut(s) 382
BseXI GCAGC 1 cut(s) 253
BseYI CCCAGC 2 cut(s) 302, 538
Bsh1236I CGCG 1 cut(s) 609
BshFI GGCC 2 cut(s) 349, 380
BshNI GGYRCC 1 cut(s) 194
BsiHKCI CYCGRG 2 cut(s) 344, 445
BsiSI CCGG 2 cut(s) 234, 446
BslI CCNNNNNNNGG 3 cut(s) 283, 338, 376
BsmAI GTCTC 2 cut(s) 347, 719
BsnI GGCC 2 cut(s) 349, 380
Bso31I GGTCTC 1 cut(s) 347
BsoBI CYCGRG 2 cut(s) 344, 445
Bsp120I GGGCCC 1 cut(s) 378
Bsp1286I GDGCHC 1 cut(s) 382
Bsp1407I TGTACA 1 cut(s) 432
Bsp143I GATC 4 cut(s) 324, 471, 514, 670
Bsp68I TCGCGA 1 cut(s) 609
BspANI GGCC 2 cut(s) 349, 380
BspCNI CTCAG 1 cut(s) 295
BspFNI CGCG 1 cut(s) 609
BspHI TCATGA 1 cut(s) 459
BspLI GGNNCC 3 cut(s) 196, 379, 380
BspPI GGATC 2 cut(s) 479, 678
BspT107I GGYRCC 1 cut(s) 194
BspTNI GGTCTC 1 cut(s) 347
BsrFI RCCGGY 1 cut(s) 233
BsrGI TGTACA 1 cut(s) 432
BsrI ACTGG 3 cut(s) 526, 584, 733
BssAI RCCGGY 1 cut(s) 233
BssECI CCNNGG 3 cut(s) 186, 445, 446
BssMI GATC 4 cut(s) 324, 471, 514, 670
BssT1I CCWWGG 1 cut(s) 186
Bst2UI CCWGG 2 cut(s) 333, 642
Bst4CI ACNGT 4 cut(s) 214, 262, 361, 571
Bst6I CTCTTC 1 cut(s) 501
BstAUI TGTACA 1 cut(s) 432
BstC8I GCNNGC 2 cut(s) 21, 632
BstDEI CTNAG 2 cut(s) 177, 282
BstENI CCTNNNNNAGG 1 cut(s) 336
BstF5I GGATG 2 cut(s) 123, 366
BstFNI CGCG 1 cut(s) 609
BstKTI GATC 4 cut(s) 327, 474, 517, 673
BstMAI GTCTC 2 cut(s) 347, 719
BstMBI GATC 4 cut(s) 324, 471, 514, 670
BstMWI GCNNNNNNNGC 3 cut(s) 20, 173, 243
BstNI CCWGG 2 cut(s) 333, 642
BstNSI RCATGY 1 cut(s) 157
BstSCI CCNGG 4 cut(s) 331, 444, 445, 640
BstSLI GKGCMC 1 cut(s) 382
BstUI CGCG 1 cut(s) 609
BstV1I GCAGC 1 cut(s) 253
BstV2I GAAGAC 1 cut(s) 470
BstX2I RGATCY 2 cut(s) 324, 471
BstYI RGATCY 2 cut(s) 324, 471
BsuRI GGCC 2 cut(s) 349, 380
BtsCI GGATG 2 cut(s) 123, 366
BtsIMutI CAGTG 2 cut(s) 258, 680
BtuMI TCGCGA 1 cut(s) 609
Cac8I GCNNGC 2 cut(s) 21, 632
CaiI CAGNNNCTG 1 cut(s) 579
CciI TCATGA 1 cut(s) 459
Cfr10I RCCGGY 1 cut(s) 233
Cfr13I GGNCC 3 cut(s) 347, 378, 379
Cfr9I CCCGGG 1 cut(s) 445
CseI GACGC 1 cut(s) 233
Csp6I GTAC 1 cut(s) 433
CviAII CATG 6 cut(s) 46, 154, 460, 468, 532, 695
CviQI GTAC 1 cut(s) 433
DdeI CTNAG 2 cut(s) 177, 282
DpnI GATC 4 cut(s) 326, 473, 516, 672
DpnII GATC 4 cut(s) 324, 471, 514, 670
DraIII CACNNNGTG 1 cut(s) 91
Eam1104I CTCTTC 1 cut(s) 501
EarI CTCTTC 1 cut(s) 501
Eco130I CCWWGG 1 cut(s) 186
Eco24I GRGCYC 1 cut(s) 382
Eco31I GGTCTC 1 cut(s) 347
Eco88I CYCGRG 2 cut(s) 344, 445
EcoNI CCTNNNNNAGG 1 cut(s) 336
EcoO109I RGGNCCY 2 cut(s) 378, 379
EcoRII CCWGG 2 cut(s) 331, 640
EcoT14I CCWWGG 1 cut(s) 186
EcoT38I GRGCYC 1 cut(s) 382
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 6 cut(s) 49, 157, 463, 471, 535, 698
FatI CATG 6 cut(s) 45, 153, 459, 467, 531, 694
FblI GTMKAC 1 cut(s) 600
Fnu4HI GCNGC 2 cut(s) 12, 267
FokI GGATG 2 cut(s) 110, 353
FriOI GRGCYC 1 cut(s) 382
Fsp4HI GCNGC 2 cut(s) 12, 267
FspBI CTAG 1 cut(s) 114
GluI GCNGC 2 cut(s) 12, 267
GsaI CCCAGC 2 cut(s) 306, 542
HaeIII GGCC 2 cut(s) 349, 380
HapII CCGG 2 cut(s) 234, 446
HgaI GACGC 1 cut(s) 233
Hin1II CATG 6 cut(s) 49, 157, 463, 471, 535, 698
HincII GTYRAC 4 cut(s) 364, 492, 601, 721
HindII GTYRAC 4 cut(s) 364, 492, 601, 721
HinfI GANTC 4 cut(s) 249, 273, 654, 691
HpaI GTTAAC 2 cut(s) 492, 721
HpaII CCGG 2 cut(s) 234, 446
HphI GGTGA 4 cut(s) 131, 202, 223, 577
Hpy166II GTNNAC 5 cut(s) 364, 492, 601, 700, 721
Hpy188I TCNGA 5 cut(s) 28, 223, 514, 519, 690
Hpy188III TCNNGA 6 cut(s) 60, 73, 107, 460, 574, 608
Hpy8I GTNNAC 5 cut(s) 364, 492, 601, 700, 721
HpyAV CCTTC 1 cut(s) 383
HpyCH4III ACNGT 4 cut(s) 214, 262, 361, 571
HpyCH4IV ACGT 1 cut(s) 352
HpyCH4V TGCA 2 cut(s) 407, 531
HpyF10VI GCNNNNNNNGC 3 cut(s) 20, 173, 243
HpyF3I CTNAG 2 cut(s) 177, 282
HpySE526I ACGT 1 cut(s) 352
Hsp92II CATG 6 cut(s) 49, 157, 463, 471, 535, 698
KspAI GTTAAC 2 cut(s) 492, 721
Kzo9I GATC 4 cut(s) 324, 471, 514, 670
LmnI GCTCC 1 cut(s) 450
Lsp1109I GCAGC 1 cut(s) 253
LweI GCATC 1 cut(s) 32
MaeI CTAG 1 cut(s) 114
MaeII ACGT 1 cut(s) 352
MaeIII GTNAC 3 cut(s) 119, 412, 565
MalI GATC 4 cut(s) 326, 473, 516, 672
MboI GATC 4 cut(s) 324, 471, 514, 670
MboII GAAGA 3 cut(s) 475, 488, 677
MfeI CAATTG 1 cut(s) 635
MflI RGATCY 2 cut(s) 324, 471
MhlI GDGCHC 1 cut(s) 382
MluCI AATT 2 cut(s) 77, 635
MlyI GAGTC 1 cut(s) 700
MnlI CCTC 6 cut(s) 137, 149, 216, 368, 555, 619
MseI TTAA 3 cut(s) 68, 491, 720
MslI CAYNNNNRTG 2 cut(s) 46, 152
MspI CCGG 2 cut(s) 234, 446
MspR9I CCNGG 4 cut(s) 333, 446, 447, 642
MunI CAATTG 1 cut(s) 635
MvaI CCWGG 2 cut(s) 333, 642
MvnI CGCG 1 cut(s) 609
MwoI GCNNNNNNNGC 3 cut(s) 20, 173, 243
NciI CCSGG 2 cut(s) 446, 447
NdeII GATC 4 cut(s) 324, 471, 514, 670
NlaIII CATG 6 cut(s) 49, 157, 463, 471, 535, 698
NlaIV GGNNCC 3 cut(s) 196, 379, 380
NmuCI GTSAC 2 cut(s) 119, 565
NruI TCGCGA 1 cut(s) 609
NspI RCATGY 1 cut(s) 157
PagI TCATGA 1 cut(s) 459
PciI ACATGT 1 cut(s) 153
PcsI WCGNNNNNNNCGW 1 cut(s) 606
PfeI GAWTC 3 cut(s) 249, 273, 654
PfoI TCCNGGA 1 cut(s) 331
PkrI GCNGC 2 cut(s) 13, 268
PleI GAGTC 1 cut(s) 699
PpsI GAGTC 1 cut(s) 699
PscI ACATGT 1 cut(s) 153
Psp6I CCWGG 2 cut(s) 331, 640
PspFI CCCAGC 2 cut(s) 302, 538
PspGI CCWGG 2 cut(s) 331, 640
PspN4I GGNNCC 3 cut(s) 196, 379, 380
PspOMI GGGCCC 1 cut(s) 378
PspPI GGNCC 3 cut(s) 347, 378, 379
PstNI CAGNNNCTG 1 cut(s) 579
PsuI RGATCY 2 cut(s) 324, 471
RruI TCGCGA 1 cut(s) 609
RsaI GTAC 1 cut(s) 434
RsaNI GTAC 1 cut(s) 433
RseI CAYNNNNRTG 2 cut(s) 46, 152
SalI GTCGAC 1 cut(s) 599
SaqAI TTAA 3 cut(s) 68, 491, 720
SatI GCNGC 2 cut(s) 12, 267
Sau3AI GATC 4 cut(s) 324, 471, 514, 670
Sau96I GGNCC 3 cut(s) 347, 378, 379
SchI GAGTC 1 cut(s) 700
ScrFI CCNGG 4 cut(s) 333, 446, 447, 642
SduI GDGCHC 1 cut(s) 382
SfaNI GCATC 1 cut(s) 32
SgrAI CRCCGGYG 1 cut(s) 233
SmaI CCCGGG 1 cut(s) 447
SmiMI CAYNNNNRTG 2 cut(s) 46, 152
SmlI CTYRAG 1 cut(s) 475
SmoI CTYRAG 1 cut(s) 475
Sse9I AATT 2 cut(s) 77, 635
SspMI CTAG 1 cut(s) 114
StyD4I CCNGG 4 cut(s) 331, 444, 445, 640
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 4 cut(s) 214, 262, 361, 571
TaiI ACGT 1 cut(s) 355
TaqI TCGA 2 cut(s) 373, 600
TasI AATT 2 cut(s) 77, 635
TatI WGTACW 1 cut(s) 432
TfiI GAWTC 3 cut(s) 249, 273, 654
Tru1I TTAA 3 cut(s) 68, 491, 720
Tru9I TTAA 3 cut(s) 68, 491, 720
TscAI CASTG 2 cut(s) 265, 680
TseFI GTSAC 2 cut(s) 119, 565
TseI GCWGC 2 cut(s) 11, 266
Tsp45I GTSAC 2 cut(s) 119, 565
TspDTI ATGAA 5 cut(s) 34, 448, 476, 488, 548
TspMI CCCGGG 1 cut(s) 445
TspRI CASTG 2 cut(s) 265, 680
XagI CCTNNNNNAGG 1 cut(s) 336
XapI RAATTY 1 cut(s) 77
XceI RCATGY 1 cut(s) 157
XmaI CCCGGG 1 cut(s) 445
XmiI GTMKAC 1 cut(s) 600
XspI CTAG 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.