MD15G1257400.v1.1

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014244
Physical Location & Seq
Forward (+)
21892982 .. 21896304
3323 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1257400.v1.1.491

Sequence Viewer

Length: 711 bp
ATGGGGGGGATTGGTTTGCTCGCGTCGGATTTGGTAATCTCGTTCATGTGGGTATGGTCGGGGGTCCTGATTAAGATCTACGTGTTTAGCTTTCTGGGGTTTGGTCACGGACCCAGCGGTGAGATAATCAAGTGCGCCCTTTCGATTTTGAACATGTTTTTCTTTGCGTTGCTGGGTAAGGTTACCAACGGTGGCACCTACAATCCTTTGACCGTGTTGTCCGGCGCGTTTACCGGGGGTTTCAGCCGCTTTCTTTTCACTGTTGCTGCCAGAATCCCTGCTCAGGTTGTTGGCTCTATTGCTGGGGTTAGGTTCATAATCGCGACCTTTCCTGAAATAGGTCTCGGGCCTCGTTTGAACGTTGACATCCATCGAGGTGCTCTTACAGAAGGATTGCTGACATTTGCAATTGTTACTATCTCGCTTGGGCTCGCCAGAAAAATCCCAGGGAGTTTCTTCACCAAGACTTGGATCTCAAGTGTTTCTAAGTTAACTCTTCACATTCTTGGCTCTGATCTGACTGGTGGTTGTATGAGCCCAGCCTCTGTGCTGGGATGGGCTTATGCTCGTGGCGACCATATCACGAAGGAGCATATCATGGTTTACTGGCTCGCCCCAATAGAGGCGACTCTGCTGGCAATATGGACATTTAGGGTCGTCGTTCCACCAGTCCAAGAGGAGAAGGCAGACAAGAAGGCTAAATCAGAATGA

Protein Analysis

237

Amino Acids

25.36

Weight (kDa)

9.48

Isoelectric Point (pI)

30.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014244)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56950 AT3G56950
fragaria_vesca FvH4_1g24870
malus_domestica MD02G1144000.v1.1 MD15G1257400.v1.1
prunus_persica Prupe.7G020600_v2.0.a1
pyrus_communis pycom02g11390 pycom15g22520
rosa_chinensis RchiOBHm_Chr2g0122531
rosa_laevigata RLG00000018682
rosa_roxburghii Rroxscaffold_2G00121500
rosa_rugosa Rorug02G0239300
rosa_samantha Rh2AG298000 Rh2BG306700 Rh2CG284800 Rh2DG321600
rosa_wichuraiana Rw2G023690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 217
AccB1I GGYRCC 1 cut(s) 194
AccB7I CCANNNNNTGG 1 cut(s) 468
AccII CGCG 3 cut(s) 23, 227, 323
AciI CCGC 2 cut(s) 117, 247
AclI AACGTT 1 cut(s) 360
AclWI GGATC 1 cut(s) 479
AfiI CCNNNNNNNGG 4 cut(s) 283, 338, 468, 622
AflIII ACRYGT 2 cut(s) 81, 153
AgsI TTSAA 2 cut(s) 151, 358
AjnI CCWGG 1 cut(s) 445
AluBI AGCT 1 cut(s) 90
AluI AGCT 1 cut(s) 90
Alw21I GWGCWC 1 cut(s) 382
Alw26I GTCTC 1 cut(s) 347
AlwI GGATC 1 cut(s) 479
AlwNI CAGNNNCTG 1 cut(s) 545
Ama87I CYCGRG 1 cut(s) 344
AoxI GGCC 1 cut(s) 347
ApeKI GCWGC 1 cut(s) 266
AspLEI GCGC 2 cut(s) 137, 227
AspS9I GGNCC 3 cut(s) 64, 110, 347
AsuC2I CCSGG 1 cut(s) 235
AsuHPI GGTGA 2 cut(s) 131, 451
AvaI CYCGRG 1 cut(s) 344
AvaII GGWCC 2 cut(s) 64, 110
BanI GGYRCC 1 cut(s) 194
BanII GRGCYC 2 cut(s) 432, 539
BauI CACGAG 1 cut(s) 567
Bbv12I GWGCWC 1 cut(s) 382
BbvI GCAGC 1 cut(s) 253
BccI CCATC 2 cut(s) 378, 549
BciT130I CCWGG 1 cut(s) 447
BcnI CCSGG 1 cut(s) 235
BcoDI GTCTC 1 cut(s) 347
BglII AGATCT 1 cut(s) 75
BisI GCNGC 2 cut(s) 247, 267
BlsI GCNGC 2 cut(s) 248, 268
Bme1390I CCNGG 2 cut(s) 235, 447
Bme18I GGWCC 2 cut(s) 64, 110
BmeT110I CYCGRG 1 cut(s) 344
BmgT120I GGNCC 3 cut(s) 64, 110, 347
BmiI GGNNCC 3 cut(s) 65, 112, 196
BmrFI CCNGG 2 cut(s) 235, 447
Bpu10I CCTNAGC 1 cut(s) 282
BpuEI CTTGAG 1 cut(s) 460
BpuMI CCSGG 1 cut(s) 235
BsaAI YACGTR 1 cut(s) 82
BsaBI GATNNNNATC 1 cut(s) 74
BsaI GGTCTC 1 cut(s) 347
BsaJI CCNNGG 3 cut(s) 234, 445, 446
Bsc4I CCNNNNNNNGG 4 cut(s) 283, 338, 468, 622
Bse1I ACTGG 3 cut(s) 526, 611, 668
Bse8I GATNNNNATC 1 cut(s) 74
BseBI CCWGG 1 cut(s) 447
BseDI CCNNGG 3 cut(s) 234, 445, 446
BseGI GGATG 2 cut(s) 366, 560
BseJI GATNNNNATC 1 cut(s) 74
BseLI CCNNNNNNNGG 4 cut(s) 283, 338, 468, 622
BseMII CTCAG 1 cut(s) 296
BseNI ACTGG 3 cut(s) 526, 611, 668
BseRI GAGGAG 1 cut(s) 692
BseXI GCAGC 1 cut(s) 253
BseYI CCCAGC 5 cut(s) 113, 172, 302, 538, 550
Bsh1236I CGCG 3 cut(s) 23, 227, 323
BshFI GGCC 1 cut(s) 349
BshNI GGYRCC 1 cut(s) 194
BsiHKAI GWGCWC 1 cut(s) 382
BsiHKCI CYCGRG 1 cut(s) 344
BsiSI CCGG 2 cut(s) 222, 234
BslI CCNNNNNNNGG 4 cut(s) 283, 338, 468, 622
BsmAI GTCTC 1 cut(s) 347
BsnI GGCC 1 cut(s) 349
Bso31I GGTCTC 1 cut(s) 347
BsoBI CYCGRG 1 cut(s) 344
Bsp1286I GDGCHC 3 cut(s) 382, 432, 539
Bsp143I GATC 3 cut(s) 75, 471, 514
Bsp68I TCGCGA 1 cut(s) 323
BspACI CCGC 2 cut(s) 117, 247
BspANI GGCC 1 cut(s) 349
BspCNI CTCAG 1 cut(s) 295
BspFNI CGCG 3 cut(s) 23, 227, 323
BspLI GGNNCC 3 cut(s) 65, 112, 196
BspPI GGATC 1 cut(s) 479
BspT107I GGYRCC 1 cut(s) 194
BspTNI GGTCTC 1 cut(s) 347
BsrI ACTGG 3 cut(s) 526, 611, 668
BssECI CCNNGG 3 cut(s) 234, 445, 446
BssMI GATC 3 cut(s) 75, 471, 514
BssSI CACGAG 1 cut(s) 567
Bst2BI CACGAG 1 cut(s) 567
Bst2UI CCWGG 1 cut(s) 447
Bst4CI ACNGT 3 cut(s) 191, 214, 262
Bst6I CTCTTC 1 cut(s) 501
BstBAI YACGTR 1 cut(s) 82
BstC8I GCNNGC 4 cut(s) 21, 432, 612, 636
BstDEI CTNAG 2 cut(s) 282, 486
BstEII GGTNACC 1 cut(s) 181
BstENI CCTNNNNNAGG 1 cut(s) 336
BstF5I GGATG 2 cut(s) 366, 560
BstFNI CGCG 3 cut(s) 23, 227, 323
BstHHI GCGC 2 cut(s) 137, 227
BstKTI GATC 3 cut(s) 78, 474, 517
BstMAI GTCTC 1 cut(s) 347
BstMBI GATC 3 cut(s) 75, 471, 514
BstNI CCWGG 1 cut(s) 447
BstNSI RCATGY 1 cut(s) 157
BstPI GGTNACC 1 cut(s) 181
BstSCI CCNGG 2 cut(s) 233, 445
BstUI CGCG 3 cut(s) 23, 227, 323
BstV1I GCAGC 1 cut(s) 253
BstX2I RGATCY 2 cut(s) 75, 471
BstYI RGATCY 2 cut(s) 75, 471
BsuRI GGCC 1 cut(s) 349
BtsCI GGATG 2 cut(s) 366, 560
BtsIMutI CAGTG 1 cut(s) 258
BtuMI TCGCGA 1 cut(s) 323
Cac8I GCNNGC 4 cut(s) 21, 432, 612, 636
CaiI CAGNNNCTG 1 cut(s) 545
CfoI GCGC 2 cut(s) 137, 227
Cfr13I GGNCC 3 cut(s) 64, 110, 347
CseI GACGC 1 cut(s) 12
CviAII CATG 3 cut(s) 46, 154, 598
DdeI CTNAG 2 cut(s) 282, 486
DpnI GATC 3 cut(s) 77, 473, 516
DpnII GATC 3 cut(s) 75, 471, 514
DrdI GACNNNNNNGTC 1 cut(s) 217
DseDI GACNNNNNNGTC 1 cut(s) 217
Eam1104I CTCTTC 1 cut(s) 501
EarI CTCTTC 1 cut(s) 501
Eco24I GRGCYC 2 cut(s) 432, 539
Eco31I GGTCTC 1 cut(s) 347
Eco47I GGWCC 2 cut(s) 64, 110
Eco88I CYCGRG 1 cut(s) 344
Eco91I GGTNACC 1 cut(s) 181
EcoNI CCTNNNNNAGG 1 cut(s) 336
EcoO109I RGGNCCY 1 cut(s) 64
EcoO65I GGTNACC 1 cut(s) 181
EcoRII CCWGG 1 cut(s) 445
EcoT38I GRGCYC 2 cut(s) 432, 539
FaeI CATG 3 cut(s) 49, 157, 601
FatI CATG 3 cut(s) 45, 153, 597
Fnu4HI GCNGC 2 cut(s) 247, 267
FokI GGATG 2 cut(s) 353, 567
FriOI GRGCYC 2 cut(s) 432, 539
Fsp4HI GCNGC 2 cut(s) 247, 267
GlaI GCGC 2 cut(s) 136, 226
GluI GCNGC 2 cut(s) 247, 267
GsaI CCCAGC 5 cut(s) 117, 176, 306, 542, 554
HaeIII GGCC 1 cut(s) 349
HapII CCGG 2 cut(s) 222, 234
HgaI GACGC 1 cut(s) 12
HhaI GCGC 2 cut(s) 137, 227
Hin1II CATG 3 cut(s) 49, 157, 601
Hin6I GCGC 2 cut(s) 135, 225
HinP1I GCGC 2 cut(s) 135, 225
HincII GTYRAC 2 cut(s) 364, 492
HindII GTYRAC 2 cut(s) 364, 492
HinfI GANTC 2 cut(s) 273, 628
HpaI GTTAAC 1 cut(s) 492
HpaII CCGG 2 cut(s) 222, 234
HphI GGTGA 2 cut(s) 131, 451
Hpy166II GTNNAC 4 cut(s) 231, 364, 492, 604
Hpy188I TCNGA 4 cut(s) 28, 514, 519, 706
Hpy188III TCNNGA 4 cut(s) 67, 322, 332, 583
Hpy8I GTNNAC 4 cut(s) 231, 364, 492, 604
Hpy99I CGWCG 2 cut(s) 28, 662
HpyAV CCTTC 4 cut(s) 383, 580, 676, 688
HpyCH4III ACNGT 3 cut(s) 191, 214, 262
HpyCH4IV ACGT 2 cut(s) 81, 360
HpyCH4V TGCA 1 cut(s) 407
HpyF3I CTNAG 2 cut(s) 282, 486
HpySE526I ACGT 2 cut(s) 81, 360
Hsp92II CATG 3 cut(s) 49, 157, 601
HspAI GCGC 2 cut(s) 135, 225
KspAI GTTAAC 1 cut(s) 492
Kzo9I GATC 3 cut(s) 75, 471, 514
LmnI GCTCC 1 cut(s) 589
Lsp1109I GCAGC 1 cut(s) 253
MaeII ACGT 2 cut(s) 81, 360
MaeIII GTNAC 3 cut(s) 104, 181, 412
MalI GATC 3 cut(s) 77, 473, 516
MboI GATC 3 cut(s) 75, 471, 514
MboII GAAGA 2 cut(s) 448, 488
MfeI CAATTG 1 cut(s) 408
MflI RGATCY 2 cut(s) 75, 471
MhlI GDGCHC 3 cut(s) 382, 432, 539
MluCI AATT 1 cut(s) 408
MlyI GAGTC 1 cut(s) 622
MmeI TCCRAC 1 cut(s) 6
MnlI CCTC 5 cut(s) 360, 368, 553, 616, 670
MseI TTAA 2 cut(s) 72, 491
MslI CAYNNNNRTG 1 cut(s) 375
MspA1I CMGCKG 1 cut(s) 117
MspI CCGG 2 cut(s) 222, 234
MspR9I CCNGG 2 cut(s) 235, 447
MunI CAATTG 1 cut(s) 408
MvaI CCWGG 1 cut(s) 447
MvnI CGCG 3 cut(s) 23, 227, 323
NciI CCSGG 1 cut(s) 235
NdeII GATC 3 cut(s) 75, 471, 514
NlaIII CATG 3 cut(s) 49, 157, 601
NlaIV GGNNCC 3 cut(s) 65, 112, 196
NmuCI GTSAC 1 cut(s) 104
NruI TCGCGA 1 cut(s) 323
NspI RCATGY 1 cut(s) 157
PasI CCCWGGG 1 cut(s) 446
PciI ACATGT 1 cut(s) 153
PfeI GAWTC 1 cut(s) 273
PflMI CCANNNNNTGG 1 cut(s) 468
PkrI GCNGC 2 cut(s) 248, 268
PleI GAGTC 1 cut(s) 622
PpsI GAGTC 1 cut(s) 622
Ppu21I YACGTR 1 cut(s) 82
PpuMI RGGWCCY 1 cut(s) 64
PscI ACATGT 1 cut(s) 153
Psp1406I AACGTT 1 cut(s) 360
Psp5II RGGWCCY 1 cut(s) 64
Psp6I CCWGG 1 cut(s) 445
PspEI GGTNACC 1 cut(s) 181
PspFI CCCAGC 5 cut(s) 113, 172, 302, 538, 550
PspGI CCWGG 1 cut(s) 445
PspN4I GGNNCC 3 cut(s) 65, 112, 196
PspPI GGNCC 3 cut(s) 64, 110, 347
PspPPI RGGWCCY 1 cut(s) 64
PstNI CAGNNNCTG 1 cut(s) 545
PsuI RGATCY 2 cut(s) 75, 471
RruI TCGCGA 1 cut(s) 323
RseI CAYNNNNRTG 1 cut(s) 375
SaqAI TTAA 2 cut(s) 72, 491
SatI GCNGC 2 cut(s) 247, 267
Sau3AI GATC 3 cut(s) 75, 471, 514
Sau96I GGNCC 3 cut(s) 64, 110, 347
SchI GAGTC 1 cut(s) 622
ScrFI CCNGG 2 cut(s) 235, 447
SduI GDGCHC 3 cut(s) 382, 432, 539
SinI GGWCC 2 cut(s) 64, 110
SmiMI CAYNNNNRTG 1 cut(s) 375
SmlI CTYRAG 1 cut(s) 475
SmoI CTYRAG 1 cut(s) 475
Sse9I AATT 1 cut(s) 408
SsiI CCGC 2 cut(s) 117, 247
StyD4I CCNGG 2 cut(s) 233, 445
TaaI ACNGT 3 cut(s) 191, 214, 262
TaiI ACGT 2 cut(s) 84, 363
TaqI TCGA 2 cut(s) 143, 373
TasI AATT 1 cut(s) 408
TauI GCSGC 1 cut(s) 249
TfiI GAWTC 1 cut(s) 273
Tru1I TTAA 2 cut(s) 72, 491
Tru9I TTAA 2 cut(s) 72, 491
TscAI CASTG 1 cut(s) 265
TseFI GTSAC 1 cut(s) 104
TseI GCWGC 1 cut(s) 266
Tsp45I GTSAC 1 cut(s) 104
TspDTI ATGAA 2 cut(s) 34, 304
TspGWI ACGGA 1 cut(s) 123
TspRI CASTG 1 cut(s) 265
Van91I CCANNNNNTGG 1 cut(s) 468
VpaK11BI GGWCC 2 cut(s) 64, 110
XagI CCTNNNNNAGG 1 cut(s) 336
XceI RCATGY 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.