AT3G56990

NUC153 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009508
Physical Location & Seq
Reverse (-)
21088027 .. 21092169
4143 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G56990.3

Sequence Viewer

Length: 2136 bp
ATGACGTCTTATGGAGATAGACTGAAGTCGACCTCCATTAATGGAGTTAAGTTATACAATGTTTCTTCTGCTCCCAATGTTCCTACTTGGCTTAATCCTAAGAAGCAACGAGCTCTCCGCAAAAACCCTCATTATATGCAAAGAGTGGAGCTGATTCAGGAGTTGAAGTTTGAGACTGCCACTACTAGAATCAAAGCGACTCCAGACGGAGAGTATCTGATTGCTTCAGGCATATATCCACCACAGGTAAAAGTTTATGAGCTAAATCAGCTTGCTTTGAAGTTTGAGAGGCATTTGGACTCGGAAATAGTTGATTTCGAGATATTGGATGATGACTTCTCAAAGCTTGCCTTCTTATGTGCTGATCGTTCTATTAATTTACACGCCAAATACGGGAAACACCACACTTTACGAATTCCAAGGATGGGGAGAGATATGACATATGATTCCTGGTCTTGTGATCTTCTTTGCGCTGCTTCCTCACCTGATCTATATAGAATCAATTTGGAGCAGGGAAGATTTCTTTCTCCTCTCAGCACTCAATCCCCAGCGCTTAATGTCGTTTCTCGAAGCAACCTCCATGGATTAGTTGCTTGTGGTGGTGAGGATGGTGCTGTGGAATTTTTTGATATGAGAATGAAATCATCTGCTGCTAGAATTAACGCGGTCACACATGGTGGTGATGCTGCTGCGGAGGTTACTGCTATAGAGTTTGATGACAGTGAAGGTCTTCAAGTAGCTGTCGGGAGTAGCGCGGGAAAGGTATTCATATACGACTTGCGTACTTCAACTCCCATAAGAGTGAAGGATCACATGTACGAGAGCCCAATTTTGAATATTAAGTGGCAACGGACTCTTAATACTCAACAACCTAAGTTGATTACTACCGACAAACACATTGTTAGAATATGGGATCCAAACACGGGAGAAGGTATGACCAGCATTGAACCGACTCAAGGAGGAATAAACGACATTTGTGTGTTCCGTGGAAGCGGACTTATGCTTCTTGCTTTAGACTCTAGCCTAATCCCATCTTACTTCATACCTGAACTCGGTCCTGCCCCCAAATGGTGTTCTCCTCTTGAAAATCTAACGGAAGAGTTGGAGGAAAGTGCACAGACAACTATTTATGATAATTACAAATTTTTGGCAATGGAAGATCTGGAGAAGTTGCAGCTAACTCATTTGATTGGAACAGATCTTTTGAAAGCATCTATGCATGGATATTTTATTAATTACCATTTGTACAAAAAGGCTTTAGCAGTGATTGAACCTTTTGCATTTGATGATTACTTAGAACGTAGGAAGCAAGAGAAGTTAGAAGAGCAGCGTACGCAGAGAATCACAAAAAAGAGACGGTTGCCAAAGGTTAACCGAGACCTTGCAGCAAGGCTTCATGGGGACGAATCAGAGGAGGAAAATAAAACTGCCGAGGATGGGGAGGCCACCAAGAAAGTGCTGAAAAAGAAGAAACCCATACTTACTGACGAGCATTTCGTAGATGGTCGGTTTGGTTCGATGTTCCAAAATCCGGACTTCCAAATTGATAAGGACTCATATGAATATGGTGTCCTACACCCTGTTGCTTCTTCGAAGAAGCAACCTTCTCTGTTAGATGAACACTTTGAAGCTGTATCAGATGATGACGAGAACAGTGATTCTGATGCATCACAGCCTTCAGATGACGAGGCCGACGATGGAGACGCAACTAGGCCAAGCAAGAAAGCGAGAACTCCGAAGTTGTATGAAGTGAAAGATGAGCGGCATGCCGCAGCTTATCACAACCGCACTTCACTGGCTAAAGAAGATAGTCTTCCTATGGGCGAGCGTGTCAAGGCTATAGAGAACCGGCGTGGCAACTTTGGAGGCTCGAAAGATATCAAATTCGGTCCTGGAGGATCACGGGAGTTTTCTTTCAAGGCGAGAGGGTCATCAAAGTACAAAGAAGATAGAGACGATGAGTATGAAGATGGGCAAAGAAACAAGAGGAGAGGAGTTCAGTCTCTTGGACTGAAATCAACAAATATTAGAGGCGGTTTCAGAGGTAGAGGAGGTGGTGGTTTTAGAGGGAGAGGAGGCGGCGGTTCCCGGGGAAAAGGTGGCCGTGGTGGTGGGCGTGGAAGAGGCCGGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUC153

Protein Analysis

711

Amino Acids

79.67

Weight (kDa)

7.3

Isoelectric Point (pI)

42.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_NOL10_N PF23098 13 - 375 5e-58 Nucleolar protein 10-like, N-terminal domain
WD40_CDC20-Fz PF24807 151 - 316 1.5e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 160 - 332 2.4e-09 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 161 - 296 1.3e-07 WDR36/Utp21 first beta-propeller
Beta-prop_THOC3 PF25174 178 - 317 9.3e-06 THOC3 beta-propeller domain
WD40_Prp19 PF24814 196 - 268 1.9e-06 Prp19 WD40 domain
NOL10_2nd PF23097 376 - 423 1.7e-18 Nucleolar protein 10-like, second domain
NUC153 PF08159 501 - 528 6.7e-09 NUC153 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 8
AccBSI CCGCTC 1 cut(s) 1762
AccI GTMKAC 1 cut(s) 29
AccII CGCG 2 cut(s) 665, 755
AccIII TCCGGA 1 cut(s) 1531
AclWI GGATC 4 cut(s) 816, 908, 921, 1906
AcoI YGGCCR 1 cut(s) 2101
AcsI RAATTY 4 cut(s) 414, 620, 1142, 1883
AcuI CTGAAG 3 cut(s) 44, 210, 1662
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 677
AfaI GTAC 5 cut(s) 784, 818, 1247, 1333, 1940
AfeI AGCGCT 1 cut(s) 552
AfiI CCNNNNNNNGG 8 cut(s) 393, 425, 923, 956, 1052, 1068, 1437, 1531
AflIII ACRYGT 1 cut(s) 813
AjnI CCWGG 2 cut(s) 449, 1891
AluBI AGCT 9 cut(s) 113, 151, 262, 271, 346, 740, 1177, 1631, 1775
AluI AGCT 9 cut(s) 113, 151, 262, 271, 346, 740, 1177, 1631, 1775
Alw21I GWGCWC 2 cut(s) 115, 1117
Alw26I GTCTC 6 cut(s) 167, 1348, 1371, 1695, 1947, 2007
Alw44I GTGCAC 1 cut(s) 1113
AlwI GGATC 4 cut(s) 816, 908, 921, 1906
Ama87I CYCGRG 1 cut(s) 2088
Aor13HI TCCGGA 1 cut(s) 1531
Aor51HI AGCGCT 1 cut(s) 552
AoxI GGCC 5 cut(s) 1443, 1689, 1712, 2101, 2125
ApaLI GTGCAC 1 cut(s) 1113
ApeKI GCWGC 8 cut(s) 473, 650, 686, 689, 1174, 1327, 1385, 1772
ApoI RAATTY 4 cut(s) 414, 620, 1142, 1883
ArsI GACNNNNNNTTYG 2 cut(s) 1478, 1510
AseI ATTAAT 3 cut(s) 39, 375, 1233
Asp700I GAANNNNTTC 1 cut(s) 729
AspLEI GCGC 3 cut(s) 473, 553, 755
AspS9I GGNCC 2 cut(s) 1055, 1889
AsuC2I CCSGG 2 cut(s) 2089, 2090
AsuHPI GGTGA 3 cut(s) 474, 614, 692
AsuII TTCGAA 1 cut(s) 1592
AvaI CYCGRG 1 cut(s) 2088
AvaII GGWCC 2 cut(s) 1055, 1889
BaeGI GKGCMC 1 cut(s) 1117
BamHI GGATCC 1 cut(s) 913
BanII GRGCYC 2 cut(s) 115, 827
BbsI GAAGAC 2 cut(s) 722, 1805
Bbv12I GWGCWC 2 cut(s) 115, 1117
BbvI GCAGC 8 cut(s) 460, 637, 673, 676, 1186, 1339, 1397, 1784
BccI CCATC 7 cut(s) 418, 602, 1039, 1430, 1496, 1691, 1964
BceAI ACGGC 1 cut(s) 2088
BciT130I CCWGG 2 cut(s) 451, 1893
BcnI CCSGG 2 cut(s) 2089, 2090
BcoDI GTCTC 6 cut(s) 167, 1348, 1371, 1695, 1947, 2007
BfaI CTAG 4 cut(s) 186, 654, 1020, 1710
BfmI CTRYAG 2 cut(s) 705, 1839
BfoI RGCGCY 1 cut(s) 554
BglII AGATCT 2 cut(s) 1159, 1198
Bme1390I CCNGG 4 cut(s) 451, 1893, 2089, 2090
Bme18I GGWCC 2 cut(s) 1055, 1889
BmeT110I CYCGRG 1 cut(s) 2088
BmgT120I GGNCC 2 cut(s) 1055, 1889
BmiI GGNNCC 2 cut(s) 915, 2086
BmrFI CCNGG 4 cut(s) 451, 1893, 2089, 2090
BmsI GCATC 4 cut(s) 673, 1220, 1654, 1676
BoxI GACNNNNGTC 1 cut(s) 25
BpiI GAAGAC 2 cut(s) 722, 1805
BpmI CTGGAG 3 cut(s) 186, 1184, 1914
Bpu14I TTCGAA 1 cut(s) 1592
BpuEI CTTGAG 1 cut(s) 939
BpuMI CCSGG 2 cut(s) 2089, 2090
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 1371
BsaJI CCNNGG 7 cut(s) 419, 580, 985, 1431, 2088, 2089, 2104
BsaWI WCCGGW 1 cut(s) 1531
BsaXI ACNNNNNCTCC 6 cut(s) 99, 129, 152, 182, 775, 805
Bsc4I CCNNNNNNNGG 8 cut(s) 393, 425, 923, 956, 1052, 1068, 1437, 1531
Bse118I RCCGGY 2 cut(s) 1848, 2127
Bse1I ACTGG 1 cut(s) 1800
Bse3DI GCAATG 1 cut(s) 1158
BseAI TCCGGA 1 cut(s) 1531
BseBI CCWGG 2 cut(s) 451, 1893
BseDI CCNNGG 7 cut(s) 419, 580, 985, 1431, 2088, 2089, 2104
BseGI GGATG 4 cut(s) 334, 429, 613, 1441
BseLI CCNNNNNNNGG 8 cut(s) 393, 425, 923, 956, 1052, 1068, 1437, 1531
BseMI GCAATG 1 cut(s) 1158
BseMII CTCAG 1 cut(s) 547
BseNI ACTGG 1 cut(s) 1800
BseRI GAGGAG 7 cut(s) 519, 1068, 1427, 2002, 2007, 2064, 2088
BseSI GKGCMC 1 cut(s) 1117
BseXI GCAGC 8 cut(s) 460, 637, 673, 676, 1186, 1339, 1397, 1784
BseYI CCCAGC 1 cut(s) 547
Bsh1236I CGCG 2 cut(s) 665, 755
BshFI GGCC 5 cut(s) 1445, 1691, 1714, 2103, 2127
BsiHKAI GWGCWC 2 cut(s) 115, 1117
BsiHKCI CYCGRG 1 cut(s) 2088
BsiSI CCGG 4 cut(s) 1532, 1849, 2089, 2128
BsiWI CGTACG 1 cut(s) 1331
BslFI GGGAC 1 cut(s) 1415
BslI CCNNNNNNNGG 8 cut(s) 393, 425, 923, 956, 1052, 1068, 1437, 1531
BsmAI GTCTC 6 cut(s) 167, 1348, 1371, 1695, 1947, 2007
BsmBI CGTCTC 3 cut(s) 1348, 1695, 1947
BsmFI GGGAC 1 cut(s) 1415
BsnI GGCC 5 cut(s) 1445, 1691, 1714, 2103, 2127
Bso31I GGTCTC 1 cut(s) 1371
BsoBI CYCGRG 1 cut(s) 2088
Bsp119I TTCGAA 1 cut(s) 1592
Bsp1286I GDGCHC 3 cut(s) 115, 827, 1117
Bsp13I TCCGGA 1 cut(s) 1531
Bsp1407I TGTACA 1 cut(s) 1245
Bsp143I GATC 8 cut(s) 364, 460, 487, 808, 913, 1159, 1198, 1898
Bsp19I CCATGG 1 cut(s) 580
BspANI GGCC 5 cut(s) 1445, 1691, 1714, 2103, 2127
BspCNI CTCAG 1 cut(s) 546
BspEI TCCGGA 1 cut(s) 1531
BspFNI CGCG 2 cut(s) 665, 755
BspLI GGNNCC 2 cut(s) 915, 2086
BspPI GGATC 4 cut(s) 816, 908, 921, 1906
BspQI GCTCTTC 1 cut(s) 1317
BspT104I TTCGAA 1 cut(s) 1592
BspTNI GGTCTC 1 cut(s) 1371
BsrBI CCGCTC 1 cut(s) 1762
BsrDI GCAATG 1 cut(s) 1158
BsrFI RCCGGY 2 cut(s) 1848, 2127
BsrGI TGTACA 1 cut(s) 1245
BsrI ACTGG 1 cut(s) 1800
BssAI RCCGGY 2 cut(s) 1848, 2127
BssECI CCNNGG 7 cut(s) 419, 580, 985, 1431, 2088, 2089, 2104
BssMI GATC 8 cut(s) 364, 460, 487, 808, 913, 1159, 1198, 1898
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 419, 580
Bst2UI CCWGG 2 cut(s) 451, 1893
Bst4CI ACNGT 3 cut(s) 722, 1359, 1655
Bst6I CTCTTC 3 cut(s) 1092, 1317, 2116
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 1245
BstBI TTCGAA 1 cut(s) 1592
BstC8I GCNNGC 5 cut(s) 273, 348, 1767, 1826, 2129
BstDEI CTNAG 4 cut(s) 99, 533, 873, 1294
BstDSI CCRYGG 3 cut(s) 580, 985, 2104
BstF5I GGATG 4 cut(s) 334, 429, 613, 1441
BstFNI CGCG 2 cut(s) 665, 755
BstH2I RGCGCY 1 cut(s) 554
BstHHI GCGC 3 cut(s) 473, 553, 755
BstKTI GATC 8 cut(s) 367, 463, 490, 811, 916, 1162, 1201, 1901
BstMAI GTCTC 6 cut(s) 167, 1348, 1371, 1695, 1947, 2007
BstMBI GATC 8 cut(s) 364, 460, 487, 808, 913, 1159, 1198, 1898
BstMWI GCNNNNNNNGC 2 cut(s) 268, 1333
BstNI CCWGG 2 cut(s) 451, 1893
BstNSI RCATGY 2 cut(s) 817, 1769
BstPAI GACNNNNGTC 1 cut(s) 25
BstSCI CCNGG 4 cut(s) 449, 1891, 2087, 2088
BstSFI CTRYAG 2 cut(s) 705, 1839
BstSLI GKGCMC 1 cut(s) 1117
BstUI CGCG 2 cut(s) 665, 755
BstV1I GCAGC 8 cut(s) 460, 637, 673, 676, 1186, 1339, 1397, 1784
BstV2I GAAGAC 2 cut(s) 722, 1805
BstX2I RGATCY 3 cut(s) 913, 1159, 1198
BstYI RGATCY 3 cut(s) 913, 1159, 1198
BsuRI GGCC 5 cut(s) 1445, 1691, 1714, 2103, 2127
BtgI CCRYGG 3 cut(s) 580, 985, 2104
BtsCI GGATG 4 cut(s) 334, 429, 613, 1441
BtsI GCAGTG 1 cut(s) 1269
BtsIMutI CAGTG 4 cut(s) 727, 1269, 1660, 1793
Cac8I GCNNGC 5 cut(s) 273, 348, 1767, 1826, 2129
CfoI GCGC 3 cut(s) 473, 553, 755
Cfr10I RCCGGY 2 cut(s) 1848, 2127
Cfr13I GGNCC 2 cut(s) 1055, 1889
Cfr9I CCCGGG 1 cut(s) 2088
CseI GACGC 1 cut(s) 1712
Csp6I GTAC 5 cut(s) 783, 817, 1246, 1332, 1939
CviAII CATG 6 cut(s) 581, 674, 814, 1220, 1397, 1766
CviQI GTAC 5 cut(s) 783, 817, 1246, 1332, 1939
DdeI CTNAG 4 cut(s) 99, 533, 873, 1294
DpnI GATC 8 cut(s) 366, 462, 489, 810, 915, 1161, 1200, 1900
DpnII GATC 8 cut(s) 364, 460, 487, 808, 913, 1159, 1198, 1898
DraIII CACNNNGTG 1 cut(s) 677
EaeI YGGCCR 1 cut(s) 2101
Eam1104I CTCTTC 3 cut(s) 1092, 1317, 2116
EarI CTCTTC 3 cut(s) 1092, 1317, 2116
Ecl136II GAGCTC 1 cut(s) 113
Eco130I CCWWGG 2 cut(s) 419, 580
Eco24I GRGCYC 2 cut(s) 115, 827
Eco31I GGTCTC 1 cut(s) 1371
Eco32I GATATC 1 cut(s) 1879
Eco47I GGWCC 2 cut(s) 1055, 1889
Eco47III AGCGCT 1 cut(s) 552
Eco53kI GAGCTC 1 cut(s) 113
Eco57I CTGAAG 3 cut(s) 44, 210, 1662
Eco88I CYCGRG 1 cut(s) 2088
EcoICRI GAGCTC 1 cut(s) 113
EcoRI GAATTC 1 cut(s) 414
EcoRII CCWGG 2 cut(s) 449, 1891
EcoRV GATATC 1 cut(s) 1879
EcoT14I CCWWGG 2 cut(s) 419, 580
EcoT22I ATGCAT 2 cut(s) 1221, 1669
EcoT38I GRGCYC 2 cut(s) 115, 827
ErhI CCWWGG 2 cut(s) 419, 580
Esp3I CGTCTC 3 cut(s) 1348, 1695, 1947
FaeI CATG 6 cut(s) 584, 677, 817, 1223, 1400, 1769
FalI AAGNNNNNCTT 4 cut(s) 335, 367, 1797, 1829
FaqI GGGAC 1 cut(s) 1415
FatI CATG 6 cut(s) 580, 673, 813, 1219, 1396, 1765
FauI CCCGC 1 cut(s) 748
FauNDI CATATG 2 cut(s) 442, 1558
FblI GTMKAC 1 cut(s) 29
FokI GGATG 4 cut(s) 341, 436, 620, 1448
FriOI GRGCYC 2 cut(s) 115, 827
FspBI CTAG 4 cut(s) 186, 654, 1020, 1710
GlaI GCGC 3 cut(s) 472, 552, 754
GsaI CCCAGC 1 cut(s) 551
GsuI CTGGAG 3 cut(s) 186, 1184, 1914
HaeII RGCGCY 1 cut(s) 554
HaeIII GGCC 5 cut(s) 1445, 1691, 1714, 2103, 2127
HapII CCGG 4 cut(s) 1532, 1849, 2089, 2128
HgaI GACGC 1 cut(s) 1712
HhaI GCGC 3 cut(s) 473, 553, 755
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 6 cut(s) 584, 677, 817, 1223, 1400, 1769
Hin6I GCGC 3 cut(s) 471, 551, 753
HinP1I GCGC 3 cut(s) 471, 551, 753
HincII GTYRAC 2 cut(s) 30, 1372
HindII GTYRAC 2 cut(s) 30, 1372
HindIII AAGCTT 1 cut(s) 344
HpaI GTTAAC 1 cut(s) 1372
HpaII CCGG 4 cut(s) 1532, 1849, 2089, 2128
HphI GGTGA 3 cut(s) 474, 614, 692
Hpy166II GTNNAC 3 cut(s) 30, 1115, 1372
Hpy188I TCNGA 8 cut(s) 219, 304, 1411, 1639, 1663, 1681, 1737, 2042
Hpy188III TCNNGA 8 cut(s) 158, 203, 319, 567, 745, 1082, 1163, 1532
Hpy8I GTNNAC 3 cut(s) 30, 1115, 1372
Hpy99I CGWCG 1 cut(s) 1697
HpyAV CCTTC 6 cut(s) 361, 719, 799, 923, 1614, 1686
HpyCH4III ACNGT 3 cut(s) 722, 1359, 1655
HpyCH4IV ACGT 2 cut(s) 5, 1300
HpyCH4V TGCA 7 cut(s) 139, 1115, 1174, 1219, 1280, 1385, 1667
HpyF10VI GCNNNNNNNGC 2 cut(s) 268, 1333
HpyF3I CTNAG 4 cut(s) 99, 533, 873, 1294
HpySE526I ACGT 2 cut(s) 5, 1300
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 6 cut(s) 584, 677, 817, 1223, 1400, 1769
HspAI GCGC 3 cut(s) 471, 551, 753
Kpn2I TCCGGA 1 cut(s) 1531
KroI GCCGGC 1 cut(s) 2127
KroNI GCCGGC 1 cut(s) 2129
KspAI GTTAAC 1 cut(s) 1372
Kzo9I GATC 8 cut(s) 364, 460, 487, 808, 913, 1159, 1198, 1898
LguI GCTCTTC 1 cut(s) 1317
LmnI GCTCC 3 cut(s) 76, 148, 508
Lsp1109I GCAGC 8 cut(s) 460, 637, 673, 676, 1186, 1339, 1397, 1784
LweI GCATC 4 cut(s) 673, 1220, 1654, 1676
MaeI CTAG 4 cut(s) 186, 654, 1020, 1710
MaeII ACGT 2 cut(s) 5, 1300
MaeIII GTNAC 2 cut(s) 667, 697
MalI GATC 8 cut(s) 366, 462, 489, 810, 915, 1161, 1200, 1900
MbiI CCGCTC 1 cut(s) 1762
MboI GATC 8 cut(s) 364, 460, 487, 808, 913, 1159, 1198, 1898
MflI RGATCY 3 cut(s) 913, 1159, 1198
MhlI GDGCHC 3 cut(s) 115, 827, 1117
MlyI GAGTC 6 cut(s) 193, 293, 847, 946, 1010, 1547
MmeI TCCRAC 1 cut(s) 1083
Mph1103I ATGCAT 2 cut(s) 1221, 1669
MroI TCCGGA 1 cut(s) 1531
MroNI GCCGGC 1 cut(s) 2127
MroXI GAANNNNTTC 1 cut(s) 729
MslI CAYNNNNRTG 3 cut(s) 678, 800, 977
MspI CCGG 4 cut(s) 1532, 1849, 2089, 2128
MspR9I CCNGG 4 cut(s) 451, 1893, 2089, 2090
MvaI CCWGG 2 cut(s) 451, 1893
MvnI CGCG 2 cut(s) 665, 755
MwoI GCNNNNNNNGC 2 cut(s) 268, 1333
NaeI GCCGGC 1 cut(s) 2129
NciI CCSGG 2 cut(s) 2089, 2090
NcoI CCATGG 1 cut(s) 580
NdeI CATATG 2 cut(s) 442, 1558
NdeII GATC 8 cut(s) 364, 460, 487, 808, 913, 1159, 1198, 1898
NgoMIV GCCGGC 1 cut(s) 2127
NlaIII CATG 6 cut(s) 584, 677, 817, 1223, 1400, 1769
NlaIV GGNNCC 2 cut(s) 915, 2086
NmeAIII GCCGAG 1 cut(s) 1456
NmuCI GTSAC 1 cut(s) 667
NsiI ATGCAT 2 cut(s) 1221, 1669
NspI RCATGY 2 cut(s) 817, 1769
NspV TTCGAA 1 cut(s) 1592
PaeI GCATGC 1 cut(s) 1769
PciI ACATGT 1 cut(s) 813
PciSI GCTCTTC 1 cut(s) 1317
PcsI WCGNNNNNNNCGW 2 cut(s) 1494, 1692
PdiI GCCGGC 1 cut(s) 2129
PdmI GAANNNNTTC 1 cut(s) 729
PfeI GAWTC 7 cut(s) 154, 189, 446, 498, 1341, 1406, 1658
Pfl23II CGTACG 1 cut(s) 1331
PfoI TCCNGGA 1 cut(s) 1891
PleI GAGTC 6 cut(s) 193, 293, 847, 946, 1010, 1547
PpsI GAGTC 6 cut(s) 193, 293, 847, 946, 1010, 1547
PscI ACATGT 1 cut(s) 813
PshAI GACNNNNGTC 1 cut(s) 25
PshBI ATTAAT 3 cut(s) 39, 375, 1233
Psp124BI GAGCTC 1 cut(s) 115
Psp6I CCWGG 2 cut(s) 449, 1891
PspFI CCCAGC 1 cut(s) 547
PspGI CCWGG 2 cut(s) 449, 1891
PspLI CGTACG 1 cut(s) 1331
PspN4I GGNNCC 2 cut(s) 915, 2086
PspPI GGNCC 2 cut(s) 1055, 1889
PsuI RGATCY 3 cut(s) 913, 1159, 1198
RsaI GTAC 5 cut(s) 784, 818, 1247, 1333, 1940
RsaNI GTAC 5 cut(s) 783, 817, 1246, 1332, 1939
RseI CAYNNNNRTG 3 cut(s) 678, 800, 977
SacI GAGCTC 1 cut(s) 115
SalI GTCGAC 1 cut(s) 28
SapI GCTCTTC 1 cut(s) 1317
Sau3AI GATC 8 cut(s) 364, 460, 487, 808, 913, 1159, 1198, 1898
Sau96I GGNCC 2 cut(s) 1055, 1889
SchI GAGTC 6 cut(s) 193, 293, 847, 946, 1010, 1547
ScrFI CCNGG 4 cut(s) 451, 1893, 2089, 2090
SduI GDGCHC 3 cut(s) 115, 827, 1117
SfaNI GCATC 4 cut(s) 673, 1220, 1654, 1676
SfcI CTRYAG 2 cut(s) 705, 1839
SfuI TTCGAA 1 cut(s) 1592
SinI GGWCC 2 cut(s) 1055, 1889
SmaI CCCGGG 1 cut(s) 2090
SmiMI CAYNNNNRTG 3 cut(s) 678, 800, 977
SmlI CTYRAG 1 cut(s) 954
SmoI CTYRAG 1 cut(s) 954
SphI GCATGC 1 cut(s) 1769
SspI AATATT 2 cut(s) 838, 2026
SspMI CTAG 4 cut(s) 186, 654, 1020, 1710
SstI GAGCTC 1 cut(s) 115
StyD4I CCNGG 4 cut(s) 449, 1891, 2087, 2088
StyI CCWWGG 2 cut(s) 419, 580
TaaI ACNGT 3 cut(s) 722, 1359, 1655
TaiI ACGT 2 cut(s) 8, 1303
TaqI TCGA 6 cut(s) 29, 318, 568, 1517, 1592, 1871
TaqII GACCGA 2 cut(s) 1043, 1877
TatI WGTACW 2 cut(s) 1245, 1938
TauI GCSGC 3 cut(s) 1765, 1772, 2082
TfiI GAWTC 7 cut(s) 154, 189, 446, 498, 1341, 1406, 1658
TscAI CASTG 4 cut(s) 727, 1269, 1660, 1800
TseFI GTSAC 1 cut(s) 667
TseI GCWGC 8 cut(s) 473, 650, 686, 689, 1174, 1327, 1385, 1772
Tsp45I GTSAC 1 cut(s) 667
TspDTI ATGAA 8 cut(s) 653, 757, 1030, 1385, 1575, 1632, 1761, 1980
TspGWI ACGGA 4 cut(s) 222, 865, 974, 1109
TspMI CCCGGG 1 cut(s) 2088
TspRI CASTG 4 cut(s) 727, 1269, 1660, 1800
VneI GTGCAC 1 cut(s) 1113
VpaK11BI GGWCC 2 cut(s) 1055, 1889
VspI ATTAAT 3 cut(s) 39, 375, 1233
XapI RAATTY 4 cut(s) 414, 620, 1142, 1883
XceI RCATGY 2 cut(s) 817, 1769
XmaI CCCGGG 1 cut(s) 2088
XmiI GTMKAC 1 cut(s) 29
XmnI GAANNNNTTC 1 cut(s) 729
XspI CTAG 4 cut(s) 186, 654, 1020, 1710
ZraI GACGTC 1 cut(s) 6
Zsp2I ATGCAT 2 cut(s) 1221, 1669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.