FvH4_2g11640

Nucleolar protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009508
Physical Location & Seq
Forward (+)
10166405 .. 10174126
7722 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g11640.t2

Sequence Viewer

Length: 2079 bp
ATGGCATACCATGGCGGCAAACTGAAGTCGACCTCCATCAATGGCGTCAAGATGTACAGCGTTGCTAATCAGCAGCCCTCTCTGGCTACCTGGCTCGACCCCAACAAGCGTCGTGCTCTCCGCAAGGACAAAAATTATATGCAAAGAGTGGACCTGATCCAGGACCTCCGCTTCGAAACCGCCACCACCAAAATCAAAGCCACCCCCGACGGCGAGTTCCTCATTGCATCTGGTATATACCCACCTCAAGTCAAAGTTTACGAGCTCAGGGAACTCTCCCTCAAGTTCGAGAGGCACCTCGACTCCGAGATTATTGATTTTCAGGTTTTGGCCGATGATTATTCCAAGCTTGCCTTTTTGTGTGCCAATCGTTCTGTTTATCTACATGCCAAATACGGAAAGCATTACAGTTTGCGGATTCCCAGAATGGGTAGGGATATGGCTTATGATTGCTGGTCATGTGATTTGCTCTGTGCTGCCTCTTCTCCTGAACTTTACAGAATTAATTTGGAACAGGGGCGATTTTTAACTTCCCTCAACACACAGTCGCCAGCGCTAAATGTAGTTTCTCGAAGCAAGCTTCATGGTTTAGTTGCTTGTGGTGGTGAGGATGGCGCTGTTGAATGTTTTGACTTGAGAATGAAATCTCCAGTTGGTAGGATAAATGCTGTGGGACCGGCTGGTGACATTGACCAGGAAGTTACAGCAGTACAATTTGATGAAAAAACGGGGTTCTTAATGGCTGTTGGAAGCAGTGCAGGGAAGGTACTCATCTATGACTTGCGTTCATCACATCCTATACAGGTTAAGGATCACATGTATGGCAGCCCAATATTAGACATTAAGTGGCATCAAACTCTTAATTCTGAACGGCCGAAGCTAATTACCACTGATAGTCACGTTGTTAGAATATGGGACCCTGATACGGGAGAAGGAATGACAAGCATTGAGCCCACGGCTGGAGCGATAAATGATATATGTACATTTCCTGGCAGTGGATTGATGCTGTTGGCTCTGGACTGCAGTCAGATACCATCGTATTTCATACCTGAACTTGGACCTGCTCCAAATTGGTGTTCTTACCTGCAAAATCTAACTGAAGAGCTAGAGGAGGGTGCACAGACGACCATCTATGATAATTTTAAATTTTTGACGAAGGAAGAACTTGAGAGGTTGAGGTTGACTAAACTGATTGGAACCAATCTACTCAGGGCTTACATGCATGGGTACTTTGTTCGTTATTCGTTGTATAAAAAGGCAAAAGCTATGGTAGAGCCTTTTGATTATAGTGAATACATTGAACAACAGAAAAAGCAGAAGCTAGAAAAAGAATTTGCAGGACAGCGAATTACGAAAAAGAGAAAATTGCCCAAGGTCAACCAACAGTTGGCAGTGAAGTTGTTGGAGTATGAAGAAACAGAAAATGAAACTAAAGATGAAAATGAGACGAACAAGCCATCCAAGAAAAAGAAGGCACTTGATAAAAATATTTTTGAAGATGAGCGATTCAAAGCAATTTTTGAAGATAAGGACTTTGAAATTGATGAGTATTCACAAGAATACAAGGCTTTACACCCAGTTTCTTCTAACAAGCGAGCATCTCTGGTGGAAGAACACTTCAGACCTATCATGGAAGATGAAGACGAGAATCATAGTGATGCAACATCAGAGGACGAGCCTGAATATGGAAACAACAGAATAAAAGGGAAAGGACGAGCTCCAAGATTGTTGGAAGTCAAGAATGAGCGGCATGCAGAGGCAGTCTGGAGTGGCGCATCACTTAGACATGAGGAGCTGCTGCCATTGGGAGAAAGGGTAAAAGCACTCGGAGATGATCAGCGGGTTTCTGCCCTCCCAAGAGGTGTTAAGCTGGGGCCCGGAGGGTCACGTGAGATTTCCTTTAGAGCTAGAGGCTCCGCAAAATATAAAGAGGATGATGATGTAGAAGCAGAGCGTGGAAAGAGACGGGGAGTTCAGTCATTGGGACTAAAACAAAATAGGAATGGATTTGGAAGCAGGGGTAGAGGTAGAGGGGGCAGAGGTGGCAGAGGGGGCAGAGGCAGAAGAGGCCGCGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUC153

Protein Analysis

693

Amino Acids

78.3

Weight (kDa)

8.54

Isoelectric Point (pI)

44.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_NOL10_N PF23098 2 - 330 7.2e-55 Nucleolar protein 10-like, N-terminal domain
WD40_CDC20-Fz PF24807 110 - 271 7.9e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 114 - 239 6.9e-09 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st PF25173 116 - 285 4.6e-07 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 123 - 222 1.5e-06 Prp19 WD40 domain
NOL10_2nd PF23097 331 - 378 2.6e-21 Nucleolar protein 10-like, second domain
NUC153 PF08159 454 - 481 2.7e-12 NUC153 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1069, 1092
AccB1I GGYRCC 1 cut(s) 294
AccB7I CCANNNNNTGG 1 cut(s) 1387
AccBSI CCGCTC 1 cut(s) 1747
AccI GTMKAC 1 cut(s) 29
AccII CGCG 1 cut(s) 2073
AclWI GGATC 2 cut(s) 151, 819
AcoI YGGCCR 2 cut(s) 330, 872
AcsI RAATTY 2 cut(s) 1145, 1331
AcuI CTGAAG 3 cut(s) 44, 1119, 1603
AcvI CACGTG 1 cut(s) 1889
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 5 cut(s) 56, 711, 768, 982, 1229
AfeI AGCGCT 1 cut(s) 555
AfiI CCNNNNNNNGG 9 cut(s) 160, 428, 925, 926, 959, 995, 1055, 1387, 1685
AflIII ACRYGT 1 cut(s) 816
AgsI TTSAA 6 cut(s) 623, 1301, 1496, 1510, 1523, 1538
AjnI CCWGG 4 cut(s) 89, 159, 693, 988
AjuI GAANNNNNNNTTGG 2 cut(s) 1060, 1092
AloI GAACNNNNNNTCC 2 cut(s) 1442, 1474
Alw21I GWGCWC 4 cut(s) 118, 267, 1120, 1720
Alw26I GTCTC 2 cut(s) 1439, 1957
Alw44I GTGCAC 1 cut(s) 1116
AlwI GGATC 2 cut(s) 151, 819
Aor51HI AGCGCT 1 cut(s) 555
AoxI GGCC 4 cut(s) 330, 872, 1874, 2068
ApaI GGGCCC 1 cut(s) 1878
ApaLI GTGCAC 1 cut(s) 1116
ApeKI GCWGC 5 cut(s) 73, 476, 825, 1795, 1798
ApoI RAATTY 2 cut(s) 1145, 1331
ArsI GACNNNNNNTTYG 2 cut(s) 155, 187
AseI ATTAAT 1 cut(s) 504
AspLEI GCGC 3 cut(s) 556, 617, 1775
AspS9I GGNCC 7 cut(s) 151, 163, 674, 916, 1058, 1874, 1875
AsuC2I CCSGG 1 cut(s) 1878
AsuHPI GGTGA 2 cut(s) 617, 695
AsuII TTCGAA 1 cut(s) 174
AvaII GGWCC 5 cut(s) 151, 163, 674, 916, 1058
BaeGI GKGCMC 2 cut(s) 1120, 1878
BaeI ACNNNNGTAYC 2 cut(s) 1022, 1055
BanI GGYRCC 1 cut(s) 294
BanII GRGCYC 4 cut(s) 267, 954, 1720, 1878
BbrPI CACGTG 1 cut(s) 1889
BbsI GAAGAC 1 cut(s) 1647
Bbv12I GWGCWC 4 cut(s) 118, 267, 1120, 1720
BbvI GCAGC 5 cut(s) 85, 463, 837, 1782, 1785
BccI CCATC 5 cut(s) 44, 605, 1042, 1136, 1465
BceAI ACGGC 3 cut(s) 226, 887, 972
BciT130I CCWGG 4 cut(s) 91, 161, 695, 990
BclI TGATCA 1 cut(s) 1834
BcnI CCSGG 1 cut(s) 1878
BcoDI GTCTC 2 cut(s) 1439, 1957
BfaI CTAG 3 cut(s) 1106, 1322, 1908
BfmI CTRYAG 1 cut(s) 1021
BfoI RGCGCY 2 cut(s) 557, 618
BfuAI ACCTGC 2 cut(s) 1069, 1092
BisI GCNGC 8 cut(s) 16, 74, 477, 826, 1748, 1796, 1799, 2071
BlsI GCNGC 8 cut(s) 17, 75, 478, 827, 1749, 1797, 1800, 2072
Bme1390I CCNGG 5 cut(s) 91, 161, 695, 990, 1878
Bme18I GGWCC 5 cut(s) 151, 163, 674, 916, 1058
BmgT120I GGNCC 7 cut(s) 151, 163, 674, 916, 1058, 1874, 1875
BmiI GGNNCC 8 cut(s) 296, 675, 917, 918, 1198, 1875, 1876, 1915
BmrFI CCNGG 5 cut(s) 91, 161, 695, 990, 1878
BmrI ACTGGG 1 cut(s) 1571
BmsI GCATC 6 cut(s) 236, 859, 993, 1607, 1648, 1784
BmuI ACTGGG 1 cut(s) 1571
BoxI GACNNNNGTC 1 cut(s) 1023
BpiI GAAGAC 1 cut(s) 1647
BpmI CTGGAG 3 cut(s) 633, 981, 1786
Bpu10I CCTNAGC 1 cut(s) 266
Bpu14I TTCGAA 1 cut(s) 174
BpuEI CTTGAG 4 cut(s) 231, 266, 655, 1187
BpuMI CCSGG 1 cut(s) 1878
BsaAI YACGTR 1 cut(s) 1889
BsaHI GRCGYC 1 cut(s) 45
BsaJI CCNNGG 4 cut(s) 10, 954, 1371, 2071
BsaXI ACNNNNNCTCC 4 cut(s) 287, 317, 1800, 1830
Bsc4I CCNNNNNNNGG 9 cut(s) 160, 428, 925, 926, 959, 995, 1055, 1387, 1685
Bse118I RCCGGY 1 cut(s) 676
Bse1I ACTGG 2 cut(s) 650, 1577
Bse3DI GCAATG 1 cut(s) 222
BseBI CCWGG 4 cut(s) 91, 161, 695, 990
BseDI CCNNGG 4 cut(s) 10, 954, 1371, 2071
BseGI GGATG 4 cut(s) 616, 793, 1457, 1939
BseLI CCNNNNNNNGG 9 cut(s) 160, 428, 925, 926, 959, 995, 1055, 1387, 1685
BseMI GCAATG 1 cut(s) 222
BseMII CTCAG 2 cut(s) 280, 1222
BseNI ACTGG 2 cut(s) 650, 1577
BseRI GAGGAG 2 cut(s) 1124, 1805
BseSI GKGCMC 2 cut(s) 1120, 1878
BseX3I CGGCCG 1 cut(s) 872
BseXI GCAGC 5 cut(s) 85, 463, 837, 1782, 1785
BseYI CCCAGC 1 cut(s) 1870
BsgI GTGCAG 1 cut(s) 777
Bsh1236I CGCG 1 cut(s) 2073
Bsh1285I CGRYCG 1 cut(s) 875
BshFI GGCC 4 cut(s) 332, 874, 1876, 2070
BshNI GGYRCC 1 cut(s) 294
BsiEI CGRYCG 1 cut(s) 875
BsiHKAI GWGCWC 4 cut(s) 118, 267, 1120, 1720
BsiSI CCGG 2 cut(s) 677, 1878
BslFI GGGAC 3 cut(s) 687, 929, 1998
BslI CCNNNNNNNGG 9 cut(s) 160, 428, 925, 926, 959, 995, 1055, 1387, 1685
BsmAI GTCTC 2 cut(s) 1439, 1957
BsmBI CGTCTC 2 cut(s) 1439, 1957
BsmFI GGGAC 3 cut(s) 687, 929, 1998
BsnI GGCC 4 cut(s) 332, 874, 1876, 2070
Bsp119I TTCGAA 1 cut(s) 174
Bsp120I GGGCCC 1 cut(s) 1874
Bsp1286I GDGCHC 6 cut(s) 118, 267, 954, 1120, 1720, 1878
Bsp1407I TGTACA 2 cut(s) 54, 980
Bsp143I GATC 3 cut(s) 156, 811, 1834
Bsp19I CCATGG 1 cut(s) 10
BspANI GGCC 4 cut(s) 332, 874, 1876, 2070
BspCNI CTCAG 2 cut(s) 279, 1221
BspFNI CGCG 1 cut(s) 2073
BspLI GGNNCC 8 cut(s) 296, 675, 917, 918, 1198, 1875, 1876, 1915
BspMAI CTGCAG 1 cut(s) 1025
BspMI ACCTGC 2 cut(s) 1069, 1092
BspPI GGATC 2 cut(s) 151, 819
BspQI GCTCTTC 1 cut(s) 1095
BspT104I TTCGAA 1 cut(s) 174
BspT107I GGYRCC 1 cut(s) 294
BsrBI CCGCTC 1 cut(s) 1747
BsrDI GCAATG 1 cut(s) 222
BsrFI RCCGGY 1 cut(s) 676
BsrGI TGTACA 2 cut(s) 54, 980
BsrI ACTGG 2 cut(s) 650, 1577
BssAI RCCGGY 1 cut(s) 676
BssECI CCNNGG 4 cut(s) 10, 954, 1371, 2071
BssMI GATC 3 cut(s) 156, 811, 1834
BssNI GRCGYC 1 cut(s) 45
BssT1I CCWWGG 2 cut(s) 10, 1371
Bst2UI CCWGG 4 cut(s) 91, 161, 695, 990
Bst4CI ACNGT 3 cut(s) 410, 546, 1386
Bst6I CTCTTC 3 cut(s) 487, 1095, 2059
BstACI GRCGYC 1 cut(s) 45
BstAUI TGTACA 2 cut(s) 54, 980
BstBAI YACGTR 1 cut(s) 1889
BstBI TTCGAA 1 cut(s) 174
BstC8I GCNNGC 5 cut(s) 351, 552, 578, 1596, 1752
BstDEI CTNAG 3 cut(s) 266, 1208, 1781
BstDSI CCRYGG 3 cut(s) 10, 954, 2071
BstENI CCTNNNNNAGG 1 cut(s) 158
BstF5I GGATG 4 cut(s) 616, 793, 1457, 1939
BstFNI CGCG 1 cut(s) 2073
BstH2I RGCGCY 2 cut(s) 557, 618
BstHHI GCGC 3 cut(s) 556, 617, 1775
BstKTI GATC 3 cut(s) 159, 814, 1837
BstMAI GTCTC 2 cut(s) 1439, 1957
BstMBI GATC 3 cut(s) 156, 811, 1834
BstMCI CGRYCG 1 cut(s) 875
BstMWI GCNNNNNNNGC 3 cut(s) 2043, 2052, 2067
BstNI CCWGG 4 cut(s) 91, 161, 695, 990
BstNSI RCATGY 4 cut(s) 389, 820, 1222, 1754
BstPAI GACNNNNGTC 1 cut(s) 1023
BstSCI CCNGG 5 cut(s) 89, 159, 693, 988, 1876
BstSFI CTRYAG 1 cut(s) 1021
BstSLI GKGCMC 2 cut(s) 1120, 1878
BstUI CGCG 1 cut(s) 2073
BstV1I GCAGC 5 cut(s) 85, 463, 837, 1782, 1785
BstV2I GAAGAC 1 cut(s) 1647
BstZI CGGCCG 1 cut(s) 872
BsuRI GGCC 4 cut(s) 332, 874, 1876, 2070
BtgI CCRYGG 3 cut(s) 10, 954, 2071
BtsCI GGATG 4 cut(s) 616, 793, 1457, 1939
BtsI GCAGTG 3 cut(s) 760, 1000, 1398
BtsIMutI CAGTG 4 cut(s) 760, 888, 1000, 1398
BveI ACCTGC 2 cut(s) 1069, 1092
Cac8I GCNNGC 5 cut(s) 351, 552, 578, 1596, 1752
CfoI GCGC 3 cut(s) 556, 617, 1775
Cfr10I RCCGGY 1 cut(s) 676
Cfr13I GGNCC 7 cut(s) 151, 163, 674, 916, 1058, 1874, 1875
Cfr42I CCGCGG 1 cut(s) 2074
CseI GACGC 2 cut(s) 34, 98
Csp6I GTAC 5 cut(s) 55, 710, 767, 981, 1228
CviQI GTAC 5 cut(s) 55, 710, 767, 981, 1228
DdeI CTNAG 3 cut(s) 266, 1208, 1781
DpnI GATC 3 cut(s) 158, 813, 1836
DpnII GATC 3 cut(s) 156, 811, 1834
DraI TTTAAA 1 cut(s) 1144
EaeI YGGCCR 2 cut(s) 330, 872
EagI CGGCCG 1 cut(s) 872
Eam1104I CTCTTC 3 cut(s) 487, 1095, 2059
EarI CTCTTC 3 cut(s) 487, 1095, 2059
Ecl136II GAGCTC 2 cut(s) 265, 1718
EclXI CGGCCG 1 cut(s) 872
Eco130I CCWWGG 2 cut(s) 10, 1371
Eco24I GRGCYC 4 cut(s) 267, 954, 1720, 1878
Eco47I GGWCC 5 cut(s) 151, 163, 674, 916, 1058
Eco47III AGCGCT 1 cut(s) 555
Eco52I CGGCCG 1 cut(s) 872
Eco53kI GAGCTC 2 cut(s) 265, 1718
Eco57I CTGAAG 3 cut(s) 44, 1119, 1603
Eco72I CACGTG 1 cut(s) 1889
EcoICRI GAGCTC 2 cut(s) 265, 1718
EcoNI CCTNNNNNAGG 1 cut(s) 158
EcoO109I RGGNCCY 3 cut(s) 163, 916, 1874
EcoRII CCWGG 4 cut(s) 89, 159, 693, 988
EcoT14I CCWWGG 2 cut(s) 10, 1371
EcoT22I ATGCAT 1 cut(s) 1224
EcoT38I GRGCYC 4 cut(s) 267, 954, 1720, 1878
ErhI CCWWGG 2 cut(s) 10, 1371
Esp3I CGTCTC 2 cut(s) 1439, 1957
FalI AAGNNNNNCTT 2 cut(s) 338, 370
FaqI GGGAC 3 cut(s) 687, 929, 1998
FauI CCCGC 2 cut(s) 1833, 2066
FbaI TGATCA 1 cut(s) 1834
FblI GTMKAC 1 cut(s) 29
Fnu4HI GCNGC 8 cut(s) 16, 74, 477, 826, 1748, 1796, 1799, 2071
FokI GGATG 4 cut(s) 623, 780, 1444, 1946
FriOI GRGCYC 4 cut(s) 267, 954, 1720, 1878
Fsp4HI GCNGC 8 cut(s) 16, 74, 477, 826, 1748, 1796, 1799, 2071
FspBI CTAG 3 cut(s) 1106, 1322, 1908
GlaI GCGC 3 cut(s) 555, 616, 1774
GluI GCNGC 8 cut(s) 16, 74, 477, 826, 1748, 1796, 1799, 2071
GsaI CCCAGC 1 cut(s) 1874
GsuI CTGGAG 3 cut(s) 633, 981, 1786
HaeII RGCGCY 2 cut(s) 557, 618
HaeIII GGCC 4 cut(s) 332, 874, 1876, 2070
HapII CCGG 2 cut(s) 677, 1878
HgaI GACGC 2 cut(s) 34, 98
HhaI GCGC 3 cut(s) 556, 617, 1775
Hin1I GRCGYC 1 cut(s) 45
Hin6I GCGC 3 cut(s) 554, 615, 1773
HinP1I GCGC 3 cut(s) 554, 615, 1773
HincII GTYRAC 3 cut(s) 30, 1182, 1378
HindII GTYRAC 3 cut(s) 30, 1182, 1378
HindIII AAGCTT 2 cut(s) 347, 578
HinfI GANTC 4 cut(s) 302, 418, 1506, 1648
HpaII CCGG 2 cut(s) 677, 1878
HphI GGTGA 2 cut(s) 617, 695
Hpy166II GTNNAC 6 cut(s) 30, 151, 259, 1118, 1182, 1378
Hpy188I TCNGA 6 cut(s) 307, 868, 1029, 1622, 1669, 1829
Hpy188III TCNNGA 7 cut(s) 49, 289, 488, 570, 1016, 1738, 1765
Hpy8I GTNNAC 6 cut(s) 30, 151, 259, 1118, 1182, 1378
Hpy99I CGWCG 2 cut(s) 114, 212
HpyAV CCTTC 4 cut(s) 757, 926, 1150, 1465
HpyCH4III ACNGT 3 cut(s) 410, 546, 1386
HpyCH4IV ACGT 2 cut(s) 900, 1888
HpyF10VI GCNNNNNNNGC 3 cut(s) 2043, 2052, 2067
HpyF3I CTNAG 3 cut(s) 266, 1208, 1781
HpySE526I ACGT 2 cut(s) 900, 1888
Hsp92I GRCGYC 1 cut(s) 45
HspAI GCGC 3 cut(s) 554, 615, 1773
KflI GGGWCCC 1 cut(s) 916
Ksp22I TGATCA 1 cut(s) 1834
KspI CCGCGG 1 cut(s) 2074
Kzo9I GATC 3 cut(s) 156, 811, 1834
LguI GCTCTTC 1 cut(s) 1095
LmnI GCTCC 5 cut(s) 962, 1069, 1723, 1792, 1919
Lsp1109I GCAGC 5 cut(s) 85, 463, 837, 1782, 1785
LweI GCATC 6 cut(s) 236, 859, 993, 1607, 1648, 1784
MaeI CTAG 3 cut(s) 1106, 1322, 1908
MaeII ACGT 2 cut(s) 900, 1888
MaeIII GTNAC 4 cut(s) 683, 700, 896, 1884
MalI GATC 3 cut(s) 158, 813, 1836
MbiI CCGCTC 1 cut(s) 1747
MboI GATC 3 cut(s) 156, 811, 1834
MhlI GDGCHC 6 cut(s) 118, 267, 954, 1120, 1720, 1878
MlyI GAGTC 1 cut(s) 296
MmeI TCCRAC 3 cut(s) 727, 1383, 1710
Mph1103I ATGCAT 1 cut(s) 1224
MseI TTAA 8 cut(s) 504, 527, 737, 807, 843, 861, 1143, 1866
MslI CAYNNNNRTG 2 cut(s) 819, 1656
MspA1I CMGCKG 2 cut(s) 1840, 2073
MspI CCGG 2 cut(s) 677, 1878
MspR9I CCNGG 5 cut(s) 91, 161, 695, 990, 1878
MvaI CCWGG 4 cut(s) 91, 161, 695, 990
MvnI CGCG 1 cut(s) 2073
MwoI GCNNNNNNNGC 3 cut(s) 2043, 2052, 2067
NciI CCSGG 1 cut(s) 1878
NcoI CCATGG 1 cut(s) 10
NdeII GATC 3 cut(s) 156, 811, 1834
NlaIV GGNNCC 8 cut(s) 296, 675, 917, 918, 1198, 1875, 1876, 1915
NmuCI GTSAC 3 cut(s) 683, 896, 1884
NsiI ATGCAT 1 cut(s) 1224
NspI RCATGY 4 cut(s) 389, 820, 1222, 1754
NspV TTCGAA 1 cut(s) 174
PaeI GCATGC 1 cut(s) 1754
PciI ACATGT 1 cut(s) 816
PciSI GCTCTTC 1 cut(s) 1095
PcsI WCGNNNNNNNCGW 1 cut(s) 962
PfeI GAWTC 3 cut(s) 418, 1506, 1648
PflMI CCANNNNNTGG 1 cut(s) 1387
PfoI TCCNGGA 1 cut(s) 159
PkrI GCNGC 8 cut(s) 17, 75, 478, 827, 1749, 1797, 1800, 2072
PleI GAGTC 1 cut(s) 296
PmaCI CACGTG 1 cut(s) 1889
PmlI CACGTG 1 cut(s) 1889
PpsI GAGTC 1 cut(s) 296
Ppu21I YACGTR 1 cut(s) 1889
PpuMI RGGWCCY 2 cut(s) 163, 916
PscI ACATGT 1 cut(s) 816
PshAI GACNNNNGTC 1 cut(s) 1023
PshBI ATTAAT 1 cut(s) 504
Psp124BI GAGCTC 2 cut(s) 267, 1720
Psp5II RGGWCCY 2 cut(s) 163, 916
Psp6I CCWGG 4 cut(s) 89, 159, 693, 988
PspCI CACGTG 1 cut(s) 1889
PspFI CCCAGC 1 cut(s) 1870
PspGI CCWGG 4 cut(s) 89, 159, 693, 988
PspN4I GGNNCC 8 cut(s) 296, 675, 917, 918, 1198, 1875, 1876, 1915
PspOMI GGGCCC 1 cut(s) 1874
PspPI GGNCC 7 cut(s) 151, 163, 674, 916, 1058, 1874, 1875
PspPPI RGGWCCY 2 cut(s) 163, 916
PstI CTGCAG 1 cut(s) 1025
RsaI GTAC 5 cut(s) 56, 711, 768, 982, 1229
RsaNI GTAC 5 cut(s) 55, 710, 767, 981, 1228
RseI CAYNNNNRTG 2 cut(s) 819, 1656
SacI GAGCTC 2 cut(s) 267, 1720
SacII CCGCGG 1 cut(s) 2074
SalI GTCGAC 1 cut(s) 28
SapI GCTCTTC 1 cut(s) 1095
SaqAI TTAA 8 cut(s) 504, 527, 737, 807, 843, 861, 1143, 1866
SatI GCNGC 8 cut(s) 16, 74, 477, 826, 1748, 1796, 1799, 2071
Sau3AI GATC 3 cut(s) 156, 811, 1834
Sau96I GGNCC 7 cut(s) 151, 163, 674, 916, 1058, 1874, 1875
SchI GAGTC 1 cut(s) 296
ScrFI CCNGG 5 cut(s) 91, 161, 695, 990, 1878
SduI GDGCHC 6 cut(s) 118, 267, 954, 1120, 1720, 1878
SfaNI GCATC 6 cut(s) 236, 859, 993, 1607, 1648, 1784
SfcI CTRYAG 1 cut(s) 1021
Sfr303I CCGCGG 1 cut(s) 2074
SfuI TTCGAA 1 cut(s) 174
SgrBI CCGCGG 1 cut(s) 2074
SinI GGWCC 5 cut(s) 151, 163, 674, 916, 1058
SmiMI CAYNNNNRTG 2 cut(s) 819, 1656
SmlI CTYRAG 4 cut(s) 246, 281, 634, 1166
SmoI CTYRAG 4 cut(s) 246, 281, 634, 1166
SphI GCATGC 1 cut(s) 1754
SspI AATATT 2 cut(s) 834, 1489
SspMI CTAG 3 cut(s) 1106, 1322, 1908
SstI GAGCTC 2 cut(s) 267, 1720
StyD4I CCNGG 5 cut(s) 89, 159, 693, 988, 1876
StyI CCWWGG 2 cut(s) 10, 1371
TaaI ACNGT 3 cut(s) 410, 546, 1386
TaiI ACGT 2 cut(s) 903, 1891
TaqI TCGA 6 cut(s) 29, 96, 174, 288, 300, 571
TatI WGTACW 3 cut(s) 54, 709, 980
TauI GCSGC 3 cut(s) 18, 1750, 2073
TfiI GAWTC 3 cut(s) 418, 1506, 1648
Tru1I TTAA 8 cut(s) 504, 527, 737, 807, 843, 861, 1143, 1866
Tru9I TTAA 8 cut(s) 504, 527, 737, 807, 843, 861, 1143, 1866
TscAI CASTG 4 cut(s) 760, 895, 1000, 1398
TseFI GTSAC 3 cut(s) 683, 896, 1884
TseI GCWGC 5 cut(s) 73, 476, 825, 1795, 1798
Tsp45I GTSAC 3 cut(s) 683, 896, 1884
TspDTI ATGAA 9 cut(s) 572, 656, 735, 777, 1033, 1425, 1440, 1452, 1653
TspGWI ACGGA 1 cut(s) 411
TspRI CASTG 4 cut(s) 760, 895, 1000, 1398
Van91I CCANNNNNTGG 1 cut(s) 1387
VneI GTGCAC 1 cut(s) 1116
VpaK11BI GGWCC 5 cut(s) 151, 163, 674, 916, 1058
VspI ATTAAT 1 cut(s) 504
XagI CCTNNNNNAGG 1 cut(s) 158
XapI RAATTY 2 cut(s) 1145, 1331
XceI RCATGY 4 cut(s) 389, 820, 1222, 1754
XmiI GTMKAC 1 cut(s) 29
XspI CTAG 3 cut(s) 1106, 1322, 1908
Zsp2I ATGCAT 1 cut(s) 1224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.