Rorug06G0057000

Nucleolar protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0009508
Physical Location & Seq
Forward (+)
7755525 .. 7756079
555 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0057000.1

Sequence Viewer

Length: 555 bp
ATGTTTTGTGGTGTATGTGGTCGGAGAGGAACAGTCTGCGGCATGACGGAGGAGTGTTTAACTGGCACTACTAACTACTTCATGCACTTTCTAATACTAACTATAAAGGAGTCAACTCGGTTGGTTTTTGCACGGTCAAATTCTCAATCTGGGGCTCCTATATTTGGCTTCTTGCATTTGTCGCCGTTGCGGCCGTCTGCTCCAAAGTTCACCCGAGTTACGTGGGTGCCTCCTCCGTTTCCTTGGGTAAAGATCAATATCGATGGGTCTTTTCGACATCAAGATCACGCAGGCTTTGGTGGCATTGCACGGAACAGTGACGGTGCATTTGTTCTTGCCTTTGCTTCACGTGTGCGTGTTCCTAGTGCTCTTGATGCTGAGGTGTTAGCGTTTATTAAAGCTATCAGGCTGTCCAACCTGAACGCATGGCAATACGTGTGGGTCAAAACGGATTCGACAACTGTTTTAAGGTATTTTGCTTCTCCACAATTGGTCCTTTGGCGGCTCCGTATTCAGTGGTCCAACTGTTTGTGTCACGCCCCGAATTTTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUC153

Protein Analysis

184

Amino Acids

20.71

Weight (kDa)

9.74

Isoelectric Point (pI)

52.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 86 - 159 5e-09 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 226
AciI CCGC 3 cut(s) 39, 190, 502
AcoI YGGCCR 1 cut(s) 191
AcsI RAATTY 2 cut(s) 139, 544
AcvI CACGTG 1 cut(s) 350
AfiI CCNNNNNNNGG 1 cut(s) 164
AflIII ACRYGT 2 cut(s) 349, 435
AgsI TTSAA 1 cut(s) 551
AluBI AGCT 1 cut(s) 401
AluI AGCT 1 cut(s) 401
Alw21I GWGCWC 1 cut(s) 370
Ama87I CYCGRG 1 cut(s) 213
AoxI GGCC 1 cut(s) 191
ApoI RAATTY 2 cut(s) 139, 544
ArsI GACNNNNNNTTYG 2 cut(s) 311, 343
AspS9I GGNCC 2 cut(s) 493, 519
AsuHPI GGTGA 1 cut(s) 202
AvaI CYCGRG 1 cut(s) 213
AvaII GGWCC 2 cut(s) 493, 519
BanI GGYRCC 1 cut(s) 226
BanII GRGCYC 1 cut(s) 157
BbrPI CACGTG 1 cut(s) 350
Bbv12I GWGCWC 1 cut(s) 370
BbvCI CCTCAGC 1 cut(s) 378
BccI CCATC 1 cut(s) 257
BceAI ACGGC 2 cut(s) 169, 178
BfaI CTAG 1 cut(s) 363
BglI GCCNNNNNGGC 1 cut(s) 190
BisI GCNGC 3 cut(s) 40, 191, 503
BlsI GCNGC 3 cut(s) 41, 192, 504
Bme18I GGWCC 2 cut(s) 493, 519
BmeT110I CYCGRG 1 cut(s) 213
BmgT120I GGNCC 2 cut(s) 493, 519
BmiI GGNNCC 3 cut(s) 156, 228, 506
BmsI GCATC 1 cut(s) 364
Bpu10I CCTNAGC 1 cut(s) 378
Bsa29I ATCGAT 1 cut(s) 261
BsaAI YACGTR 3 cut(s) 222, 350, 436
BsaBI GATNNNNATC 1 cut(s) 257
BsaJI CCNNGG 1 cut(s) 242
Bsc4I CCNNNNNNNGG 1 cut(s) 164
Bse1I ACTGG 1 cut(s) 67
Bse3DI GCAATG 1 cut(s) 303
Bse8I GATNNNNATC 1 cut(s) 257
BseCI ATCGAT 1 cut(s) 261
BseDI CCNNGG 1 cut(s) 242
BseJI GATNNNNATC 1 cut(s) 257
BseLI CCNNNNNNNGG 1 cut(s) 164
BseMI GCAATG 1 cut(s) 303
BseMII CTCAG 1 cut(s) 369
BseNI ACTGG 1 cut(s) 67
BseRI GAGGAG 2 cut(s) 65, 222
BseX3I CGGCCG 1 cut(s) 191
Bsh1285I CGRYCG 1 cut(s) 194
BshFI GGCC 1 cut(s) 193
BshNI GGYRCC 1 cut(s) 226
BshVI ATCGAT 1 cut(s) 261
BsiEI CGRYCG 1 cut(s) 194
BsiHKAI GWGCWC 1 cut(s) 370
BsiHKCI CYCGRG 1 cut(s) 213
BslI CCNNNNNNNGG 1 cut(s) 164
BsnI GGCC 1 cut(s) 193
BsoBI CYCGRG 1 cut(s) 213
Bsp1286I GDGCHC 2 cut(s) 157, 370
Bsp143I GATC 2 cut(s) 252, 283
BspACI CCGC 3 cut(s) 39, 190, 502
BspANI GGCC 1 cut(s) 193
BspCNI CTCAG 1 cut(s) 370
BspDI ATCGAT 1 cut(s) 261
BspLI GGNNCC 3 cut(s) 156, 228, 506
BspT107I GGYRCC 1 cut(s) 226
BsrDI GCAATG 1 cut(s) 303
BsrI ACTGG 1 cut(s) 67
BssECI CCNNGG 1 cut(s) 242
BssMI GATC 2 cut(s) 252, 283
BssT1I CCWWGG 1 cut(s) 242
Bst4CI ACNGT 6 cut(s) 34, 135, 317, 323, 463, 527
BstBAI YACGTR 3 cut(s) 222, 350, 436
BstC8I GCNNGC 1 cut(s) 292
BstDEI CTNAG 1 cut(s) 378
BstKTI GATC 2 cut(s) 255, 286
BstMBI GATC 2 cut(s) 252, 283
BstMCI CGRYCG 1 cut(s) 194
BstMWI GCNNNNNNNGC 4 cut(s) 181, 190, 300, 374
BstZI CGGCCG 1 cut(s) 191
Bsu15I ATCGAT 1 cut(s) 261
BsuRI GGCC 1 cut(s) 193
BsuTUI ATCGAT 1 cut(s) 261
BtsIMutI CAGTG 2 cut(s) 322, 521
Cac8I GCNNGC 1 cut(s) 292
Cfr13I GGNCC 2 cut(s) 493, 519
ClaI ATCGAT 1 cut(s) 261
CspCI CAANNNNNGTGG 2 cut(s) 419, 454
CviAII CATG 3 cut(s) 43, 82, 426
CviJI RGCY 7 cut(s) 155, 168, 193, 294, 401, 409, 505
CviKI_1 RGCY 7 cut(s) 155, 168, 193, 294, 401, 409, 505
DdeI CTNAG 1 cut(s) 378
DpnI GATC 2 cut(s) 254, 285
DpnII GATC 2 cut(s) 252, 283
EaeI YGGCCR 1 cut(s) 191
EagI CGGCCG 1 cut(s) 191
EclXI CGGCCG 1 cut(s) 191
Eco130I CCWWGG 1 cut(s) 242
Eco24I GRGCYC 1 cut(s) 157
Eco47I GGWCC 2 cut(s) 493, 519
Eco52I CGGCCG 1 cut(s) 191
Eco72I CACGTG 1 cut(s) 350
Eco88I CYCGRG 1 cut(s) 213
EcoT14I CCWWGG 1 cut(s) 242
EcoT38I GRGCYC 1 cut(s) 157
ErhI CCWWGG 1 cut(s) 242
FaeI CATG 3 cut(s) 46, 85, 429
FaiI YATR 6 cut(s) 16, 44, 83, 104, 161, 427
FatI CATG 3 cut(s) 42, 81, 425
Fnu4HI GCNGC 3 cut(s) 40, 191, 503
FriOI GRGCYC 1 cut(s) 157
Fsp4HI GCNGC 3 cut(s) 40, 191, 503
FspBI CTAG 1 cut(s) 363
GluI GCNGC 3 cut(s) 40, 191, 503
HaeIII GGCC 1 cut(s) 193
Hin1II CATG 3 cut(s) 46, 85, 429
HincII GTYRAC 1 cut(s) 114
HindII GTYRAC 1 cut(s) 114
HinfI GANTC 2 cut(s) 110, 452
HphI GGTGA 1 cut(s) 202
Hpy166II GTNNAC 2 cut(s) 114, 210
Hpy188I TCNGA 1 cut(s) 24
Hpy188III TCNNGA 2 cut(s) 281, 371
Hpy8I GTNNAC 2 cut(s) 114, 210
HpyCH4III ACNGT 6 cut(s) 34, 135, 317, 323, 463, 527
HpyCH4IV ACGT 3 cut(s) 221, 349, 435
HpyCH4V TGCA 5 cut(s) 85, 131, 175, 308, 326
HpyF10VI GCNNNNNNNGC 4 cut(s) 181, 190, 300, 374
HpyF3I CTNAG 1 cut(s) 378
HpySE526I ACGT 3 cut(s) 221, 349, 435
Hsp92II CATG 3 cut(s) 46, 85, 429
Kzo9I GATC 2 cut(s) 252, 283
LmnI GCTCC 3 cut(s) 160, 205, 510
LpnPI CCDG 5 cut(s) 48, 135, 276, 391, 431
LweI GCATC 1 cut(s) 364
MaeI CTAG 1 cut(s) 363
MaeII ACGT 3 cut(s) 221, 349, 435
MaeIII GTNAC 3 cut(s) 217, 317, 533
MalI GATC 2 cut(s) 254, 285
MboI GATC 2 cut(s) 252, 283
MfeI CAATTG 1 cut(s) 488
MhlI GDGCHC 2 cut(s) 157, 370
MluCI AATT 3 cut(s) 139, 488, 544
MlyI GAGTC 1 cut(s) 119
MmeI TCCRAC 2 cut(s) 438, 546
MnlI CCTC 5 cut(s) 20, 43, 240, 243, 373
MseI TTAA 3 cut(s) 59, 396, 467
MunI CAATTG 1 cut(s) 488
MwoI GCNNNNNNNGC 4 cut(s) 181, 190, 300, 374
NdeII GATC 2 cut(s) 252, 283
NlaIII CATG 3 cut(s) 46, 85, 429
NlaIV GGNNCC 3 cut(s) 156, 228, 506
NmuCI GTSAC 2 cut(s) 317, 533
PfeI GAWTC 1 cut(s) 452
PkrI GCNGC 3 cut(s) 41, 192, 504
PleI GAGTC 1 cut(s) 118
PmaCI CACGTG 1 cut(s) 350
PmlI CACGTG 1 cut(s) 350
PpsI GAGTC 1 cut(s) 118
Ppu21I YACGTR 3 cut(s) 222, 350, 436
PspCI CACGTG 1 cut(s) 350
PspN4I GGNNCC 3 cut(s) 156, 228, 506
PspPI GGNCC 2 cut(s) 493, 519
SaqAI TTAA 3 cut(s) 59, 396, 467
SatI GCNGC 3 cut(s) 40, 191, 503
Sau3AI GATC 2 cut(s) 252, 283
Sau96I GGNCC 2 cut(s) 493, 519
SchI GAGTC 1 cut(s) 119
SduI GDGCHC 2 cut(s) 157, 370
SetI ASST 7 cut(s) 224, 352, 384, 403, 420, 438, 473
SfaNI GCATC 1 cut(s) 364
SinI GGWCC 2 cut(s) 493, 519
Sse9I AATT 3 cut(s) 139, 488, 544
SsiI CCGC 3 cut(s) 39, 190, 502
SspMI CTAG 1 cut(s) 363
StyI CCWWGG 1 cut(s) 242
TaaI ACNGT 6 cut(s) 34, 135, 317, 323, 463, 527
TaiI ACGT 3 cut(s) 224, 352, 438
TaqI TCGA 3 cut(s) 261, 274, 455
TasI AATT 3 cut(s) 139, 488, 544
TauI GCSGC 3 cut(s) 42, 193, 505
TfiI GAWTC 1 cut(s) 452
Tru1I TTAA 3 cut(s) 59, 396, 467
Tru9I TTAA 3 cut(s) 59, 396, 467
TscAI CASTG 2 cut(s) 322, 521
TseFI GTSAC 2 cut(s) 317, 533
Tsp45I GTSAC 2 cut(s) 317, 533
TspDTI ATGAA 1 cut(s) 70
TspGWI ACGGA 5 cut(s) 62, 225, 325, 464, 497
TspRI CASTG 2 cut(s) 322, 521
VpaK11BI GGWCC 2 cut(s) 493, 519
XapI RAATTY 2 cut(s) 139, 544
XspI CTAG 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.