Rh6BG179900

Nucleolar protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0009508
Physical Location & Seq
Forward (+)
30924228 .. 30931379
7152 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG179900.1

Sequence Viewer

Length: 1500 bp
ATGGCTTACCATGGAGGCAATCTCAAGTCGACCTCCATCAATGGCGTCAAGATGTACAGCGTTGCTTCTCAGCAACCCTCTCTCGCCACCTGGCTTGACCCCAACAAGCGTCGAGCCCTCCGCAAAGACAAAAATTATATGCAAAGGGTGGACTTGATTCAGGACCTCCGCTTCGAAACTGCCACCACCAAAATCAAGGCCACTCCTGACGGCGAGTTCCTCATTGCGTCAGGTATATACCCGCCCCAAGTCAAGGTTTATGAGCTCAGGGAACTTGCACTCAAGTTTGAAAGGCACTTGGATTCTGAGATTATTGATTTTGAGGTTTTGGCTGATGATTATTCAAAGCTTGCGTTTTTATGTGCCGATCGTTCTGTTTATCTACATGCAAAATATGGAAAACATTACAGTTTGCGCATTCCAAGAATGGGTAGGGATATGGCTTATGATTGCTGGTCATGTGATTTGCTTTGTGCTGCCTCTTCTCCAGAACTTTACAGAATTAATTTGGAACAGGGGCGTTTTTTATCTTCCCTCAACACACAGTCACCAGCGCTAAATGTAGTTTCTCGAAGCAAGCTTCATGGTTTAGTTGCTTGTGGTGGTGAAGATGGTGCTGTGGAATGTTTTGACTTGAGAATGAAATCTTCAGTTGGTAGGATTAATGCTGTTGGACCTGCTGGTGACATCGACCAGGAGGTCACAGCAGTAGAGTTTGATGAAAAAACGGGTTTCTTAATGGCTGTTGGAAGCAGTGCAGGGAAGGTTCTCGTCTATGACTTGCGTTCATCACATCCTATACAGGTGAAGGATCACATGTATGGCAGTCCGATATTAGACATCAAGTGGCATCAAACTCTTAATTCTGAACGGCCAAAGTTAATTACCACTGACAATCACGTTGTTAGAATATGGGACCCTGACACAGGAGAAGGAATGACAAGCATTGAGCCCACAGCTGGAGCAATAAATGATATATGTACATTCCCTGGCAGTGGATTGATGCTGTTGGCTCTGGACTCCAGTCAGATACCATCGTATTTCATACCTGAACTCGGACCTGCTCCAAATTGGTGTTCTTACCTGCAAAATCTTACTGAAGAGTTAGAGGAGGGTGCGCAGACGACCATCTATGATGATTATAAATTTTTGACAAAAGAAGACCTTGAGAGGTTGAGGTTGACTAAGCTGATTGGAACGAATCTACTCAGAGCTTACATGCATGGGTTCTTTGTTCAGTATTCGTTGTATAAAAAGGTTTGGTTGCCTTCATTTATATATGATTATTTTGATCAAGGGTTTCATGACTGTTGGAACCAAAATAAATTTTTATCATCCATATGTATGCTGTCCCCGTGCTGTATTTCATATAAGGAAAGAAAGGTTGTAAATAGACCGGTTGGACTTGTTTCAGACTTAAAAAAAACAGAGGAGGATAATAATGAATCTGTTTCCATTCCTGCTCTAAATACTATTCTTAGTAATCATTTCTGGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUC153

Protein Analysis

499

Amino Acids

56.42

Weight (kDa)

5.85

Isoelectric Point (pI)

48.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_NOL10_N PF23098 14 - 376 5.1e-57 Nucleolar protein 10-like, N-terminal domain
WD40_CDC20-Fz PF24807 153 - 316 5.5e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 160 - 297 2.6e-09 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st PF25173 162 - 342 3.3e-08 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 170 - 268 1.4e-06 Prp19 WD40 domain
NOL10_2nd PF23097 377 - 420 2.6e-20 Nucleolar protein 10-like, second domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1143
AasI GACNNNNNNGTC 1 cut(s) 698
Acc16I TGCGCA 2 cut(s) 416, 1119
Acc36I ACCTGC 3 cut(s) 685, 1069, 1092
AccI GTMKAC 1 cut(s) 29
AciI CCGC 3 cut(s) 121, 169, 242
AclWI GGATC 1 cut(s) 819
AcoI YGGCCR 1 cut(s) 872
AcsI RAATTY 2 cut(s) 1145, 1325
AcuI CTGAAG 2 cut(s) 633, 1119
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 2 cut(s) 56, 982
AfeI AGCGCT 1 cut(s) 555
AfiI CCNNNNNNNGG 6 cut(s) 253, 428, 926, 959, 995, 1055
AflIII ACRYGT 1 cut(s) 816
AgeI ACCGGT 1 cut(s) 1396
AgsI TTSAA 2 cut(s) 290, 345
AjnI CCWGG 3 cut(s) 89, 693, 988
AjuI GAANNNNNNNTTGG 2 cut(s) 1060, 1092
AluBI AGCT 6 cut(s) 265, 349, 580, 959, 1189, 1214
AluI AGCT 6 cut(s) 265, 349, 580, 959, 1189, 1214
Alw21I GWGCWC 1 cut(s) 267
AlwI GGATC 1 cut(s) 819
Aor51HI AGCGCT 1 cut(s) 555
AoxI GGCC 2 cut(s) 198, 872
ApeKI GCWGC 1 cut(s) 476
ApoI RAATTY 2 cut(s) 1145, 1325
ArsI GACNNNNNNTTYG 2 cut(s) 155, 187
AseI ATTAAT 2 cut(s) 504, 663
AsiGI ACCGGT 1 cut(s) 1396
AspLEI GCGC 3 cut(s) 417, 556, 1120
AspS9I GGNCC 4 cut(s) 163, 674, 916, 1058
AsuHPI GGTGA 4 cut(s) 540, 617, 695, 817
AsuII TTCGAA 1 cut(s) 174
AvaII GGWCC 4 cut(s) 163, 674, 916, 1058
BaeI ACNNNNGTAYC 2 cut(s) 1022, 1055
BanII GRGCYC 3 cut(s) 118, 267, 954
BbsI GAAGAC 1 cut(s) 1167
Bbv12I GWGCWC 1 cut(s) 267
BbvI GCAGC 1 cut(s) 463
BccI CCATC 4 cut(s) 44, 605, 1042, 1136
BceAI ACGGC 2 cut(s) 226, 887
BciT130I CCWGG 3 cut(s) 91, 695, 990
BclI TGATCA 1 cut(s) 1291
BfoI RGCGCY 1 cut(s) 557
BfuAI ACCTGC 3 cut(s) 685, 1069, 1092
BisI GCNGC 1 cut(s) 477
BlsI GCNGC 1 cut(s) 478
Bme1390I CCNGG 3 cut(s) 91, 695, 990
Bme18I GGWCC 4 cut(s) 163, 674, 916, 1058
BmgT120I GGNCC 4 cut(s) 163, 674, 916, 1058
BmiI GGNNCC 3 cut(s) 917, 918, 1316
BmrFI CCNGG 3 cut(s) 91, 695, 990
BmsI GCATC 2 cut(s) 859, 993
BoxI GACNNNNGTC 1 cut(s) 1023
BpiI GAAGAC 1 cut(s) 1167
BplI GAGNNNNNCTC 1 cut(s) 38
BpmI CTGGAG 3 cut(s) 471, 981, 1006
Bpu10I CCTNAGC 1 cut(s) 266
Bpu14I TTCGAA 1 cut(s) 174
BpuEI CTTGAG 4 cut(s) 8, 266, 655, 1187
BsaHI GRCGYC 1 cut(s) 45
BsaJI CCNNGG 2 cut(s) 10, 988
BsaWI WCCGGW 1 cut(s) 1396
Bsc4I CCNNNNNNNGG 6 cut(s) 253, 428, 926, 959, 995, 1055
Bse118I RCCGGY 1 cut(s) 1396
Bse1I ACTGG 1 cut(s) 1023
Bse3DI GCAATG 1 cut(s) 222
BseBI CCWGG 3 cut(s) 91, 695, 990
BseDI CCNNGG 2 cut(s) 10, 988
BseGI GGATG 2 cut(s) 793, 1334
BseLI CCNNNNNNNGG 6 cut(s) 253, 428, 926, 959, 995, 1055
BseMI GCAATG 1 cut(s) 222
BseMII CTCAG 4 cut(s) 83, 280, 297, 1222
BseNI ACTGG 1 cut(s) 1023
BseRI GAGGAG 2 cut(s) 1124, 1445
BseXI GCAGC 1 cut(s) 463
BsgI GTGCAG 1 cut(s) 777
Bsh1285I CGRYCG 1 cut(s) 370
BshFI GGCC 2 cut(s) 200, 874
BshTI ACCGGT 1 cut(s) 1396
BsiEI CGRYCG 1 cut(s) 370
BsiHKAI GWGCWC 1 cut(s) 267
BsiSI CCGG 1 cut(s) 1397
BslFI GGGAC 2 cut(s) 929, 1336
BslI CCNNNNNNNGG 6 cut(s) 253, 428, 926, 959, 995, 1055
BsmFI GGGAC 2 cut(s) 929, 1336
BsmI GAATGC 1 cut(s) 417
BsnI GGCC 2 cut(s) 200, 874
Bsp119I TTCGAA 1 cut(s) 174
Bsp1286I GDGCHC 3 cut(s) 118, 267, 954
Bsp1407I TGTACA 2 cut(s) 54, 980
Bsp143I GATC 3 cut(s) 367, 811, 1291
Bsp19I CCATGG 1 cut(s) 10
BspACI CCGC 3 cut(s) 121, 169, 242
BspANI GGCC 2 cut(s) 200, 874
BspCNI CTCAG 4 cut(s) 82, 279, 298, 1221
BspHI TCATGA 1 cut(s) 1303
BspLI GGNNCC 3 cut(s) 917, 918, 1316
BspMI ACCTGC 3 cut(s) 685, 1069, 1092
BspPI GGATC 1 cut(s) 819
BspT104I TTCGAA 1 cut(s) 174
BsrDI GCAATG 1 cut(s) 222
BsrFI RCCGGY 1 cut(s) 1396
BsrGI TGTACA 2 cut(s) 54, 980
BsrI ACTGG 1 cut(s) 1023
BssAI RCCGGY 1 cut(s) 1396
BssECI CCNNGG 2 cut(s) 10, 988
BssMI GATC 3 cut(s) 367, 811, 1291
BssNI GRCGYC 1 cut(s) 45
BssT1I CCWWGG 1 cut(s) 10
Bst2UI CCWGG 3 cut(s) 91, 695, 990
Bst4CI ACNGT 3 cut(s) 410, 546, 1310
Bst6I CTCTTC 2 cut(s) 487, 1095
BstACI GRCGYC 1 cut(s) 45
BstAUI TGTACA 2 cut(s) 54, 980
BstBI TTCGAA 1 cut(s) 174
BstC8I GCNNGC 2 cut(s) 351, 578
BstDEI CTNAG 6 cut(s) 69, 266, 306, 1185, 1208, 1478
BstDSI CCRYGG 1 cut(s) 10
BstENI CCTNNNNNAGG 1 cut(s) 924
BstF5I GGATG 2 cut(s) 793, 1334
BstH2I RGCGCY 1 cut(s) 557
BstHHI GCGC 3 cut(s) 417, 556, 1120
BstKTI GATC 3 cut(s) 370, 814, 1294
BstMBI GATC 3 cut(s) 367, 811, 1291
BstMCI CGRYCG 1 cut(s) 370
BstNI CCWGG 3 cut(s) 91, 695, 990
BstNSI RCATGY 3 cut(s) 389, 820, 1222
BstPAI GACNNNNGTC 1 cut(s) 1023
BstSCI CCNGG 3 cut(s) 89, 693, 988
BstV1I GCAGC 1 cut(s) 463
BstV2I GAAGAC 1 cut(s) 1167
BsuRI GGCC 2 cut(s) 200, 874
BtgI CCRYGG 1 cut(s) 10
BtsCI GGATG 2 cut(s) 793, 1334
BtsI GCAGTG 2 cut(s) 760, 1000
BtsIMutI CAGTG 3 cut(s) 760, 888, 1000
BveI ACCTGC 3 cut(s) 685, 1069, 1092
Cac8I GCNNGC 2 cut(s) 351, 578
CciI TCATGA 1 cut(s) 1303
CfoI GCGC 3 cut(s) 417, 556, 1120
Cfr10I RCCGGY 1 cut(s) 1396
Cfr13I GGNCC 4 cut(s) 163, 674, 916, 1058
CseI GACGC 3 cut(s) 34, 98, 216
Csp6I GTAC 2 cut(s) 55, 981
CspAI ACCGGT 1 cut(s) 1396
CspCI CAANNNNNGTGG 2 cut(s) 76, 111
CviAII CATG 8 cut(s) 11, 386, 459, 584, 817, 1219, 1223, 1304
CviQI GTAC 2 cut(s) 55, 981
DdeI CTNAG 6 cut(s) 69, 266, 306, 1185, 1208, 1478
DpnI GATC 3 cut(s) 369, 813, 1293
DpnII GATC 3 cut(s) 367, 811, 1291
DrdI GACNNNNNNGTC 1 cut(s) 698
DseDI GACNNNNNNGTC 1 cut(s) 698
EaeI YGGCCR 1 cut(s) 872
Eam1104I CTCTTC 2 cut(s) 487, 1095
EarI CTCTTC 2 cut(s) 487, 1095
Ecl136II GAGCTC 1 cut(s) 265
Eco130I CCWWGG 1 cut(s) 10
Eco24I GRGCYC 3 cut(s) 118, 267, 954
Eco47I GGWCC 4 cut(s) 163, 674, 916, 1058
Eco47III AGCGCT 1 cut(s) 555
Eco53kI GAGCTC 1 cut(s) 265
Eco57I CTGAAG 2 cut(s) 633, 1119
EcoICRI GAGCTC 1 cut(s) 265
EcoNI CCTNNNNNAGG 1 cut(s) 924
EcoO109I RGGNCCY 2 cut(s) 163, 916
EcoRII CCWGG 3 cut(s) 89, 693, 988
EcoT14I CCWWGG 1 cut(s) 10
EcoT22I ATGCAT 1 cut(s) 1224
EcoT38I GRGCYC 3 cut(s) 118, 267, 954
ErhI CCWWGG 1 cut(s) 10
FaeI CATG 8 cut(s) 14, 389, 462, 587, 820, 1222, 1226, 1307
FalI AAGNNNNNCTT 2 cut(s) 1149, 1181
FaqI GGGAC 2 cut(s) 929, 1336
FatI CATG 8 cut(s) 10, 385, 458, 583, 816, 1218, 1222, 1303
FauI CCCGC 1 cut(s) 249
FauNDI CATATG 1 cut(s) 1340
FbaI TGATCA 1 cut(s) 1291
FblI GTMKAC 1 cut(s) 29
Fnu4HI GCNGC 1 cut(s) 477
FokI GGATG 2 cut(s) 780, 1321
FriOI GRGCYC 3 cut(s) 118, 267, 954
Fsp4HI GCNGC 1 cut(s) 477
FspI TGCGCA 2 cut(s) 416, 1119
GlaI GCGC 3 cut(s) 416, 555, 1119
GluI GCNGC 1 cut(s) 477
GsuI CTGGAG 3 cut(s) 471, 981, 1006
HaeII RGCGCY 1 cut(s) 557
HaeIII GGCC 2 cut(s) 200, 874
HapII CCGG 1 cut(s) 1397
HgaI GACGC 3 cut(s) 34, 98, 216
HhaI GCGC 3 cut(s) 417, 556, 1120
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 8 cut(s) 14, 389, 462, 587, 820, 1222, 1226, 1307
Hin6I GCGC 3 cut(s) 415, 554, 1118
HinP1I GCGC 3 cut(s) 415, 554, 1118
HincII GTYRAC 2 cut(s) 30, 1182
HindII GTYRAC 2 cut(s) 30, 1182
HindIII AAGCTT 2 cut(s) 347, 578
HinfI GANTC 5 cut(s) 157, 302, 1019, 1201, 1445
HpaII CCGG 1 cut(s) 1397
HphI GGTGA 4 cut(s) 540, 617, 695, 817
Hpy166II GTNNAC 3 cut(s) 30, 151, 1182
Hpy188I TCNGA 7 cut(s) 307, 831, 868, 1029, 1058, 1211, 1414
Hpy188III TCNNGA 7 cut(s) 49, 161, 206, 488, 570, 1016, 1304
Hpy8I GTNNAC 3 cut(s) 30, 151, 1182
Hpy99I CGWCG 1 cut(s) 114
HpyAV CCTTC 4 cut(s) 757, 802, 926, 1278
HpyCH4III ACNGT 3 cut(s) 410, 546, 1310
HpyCH4IV ACGT 1 cut(s) 900
HpyCH4V TGCA 6 cut(s) 142, 278, 389, 758, 1087, 1222
HpyF3I CTNAG 6 cut(s) 69, 266, 306, 1185, 1208, 1478
HpySE526I ACGT 1 cut(s) 900
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 8 cut(s) 14, 389, 462, 587, 820, 1222, 1226, 1307
HspAI GCGC 3 cut(s) 415, 554, 1118
KflI GGGWCCC 1 cut(s) 916
Ksp22I TGATCA 1 cut(s) 1291
Kzo9I GATC 3 cut(s) 367, 811, 1291
LmnI GCTCC 2 cut(s) 962, 1069
Lsp1109I GCAGC 1 cut(s) 463
LweI GCATC 2 cut(s) 859, 993
MaeII ACGT 1 cut(s) 900
MaeIII GTNAC 3 cut(s) 546, 683, 700
MalI GATC 3 cut(s) 369, 813, 1293
MboI GATC 3 cut(s) 367, 811, 1291
MboII GAAGA 6 cut(s) 474, 522, 620, 639, 1112, 1172
MhlI GDGCHC 3 cut(s) 118, 267, 954
MluCI AATT 8 cut(s) 133, 501, 505, 862, 882, 1069, 1145, 1325
MlyI GAGTC 1 cut(s) 1013
MmeI TCCRAC 4 cut(s) 652, 727, 1292, 1381
Mph1103I ATGCAT 1 cut(s) 1224
MseI TTAA 6 cut(s) 504, 663, 737, 861, 881, 1418
MslI CAYNNNNRTG 3 cut(s) 819, 1339, 1343
MspA1I CMGCKG 1 cut(s) 959
MspI CCGG 1 cut(s) 1397
MspR9I CCNGG 3 cut(s) 91, 695, 990
Mva1269I GAATGC 1 cut(s) 417
MvaI CCWGG 3 cut(s) 91, 695, 990
NcoI CCATGG 1 cut(s) 10
NdeI CATATG 1 cut(s) 1340
NdeII GATC 3 cut(s) 367, 811, 1291
NlaIII CATG 8 cut(s) 14, 389, 462, 587, 820, 1222, 1226, 1307
NlaIV GGNNCC 3 cut(s) 917, 918, 1316
NmuCI GTSAC 3 cut(s) 546, 683, 700
NsbI TGCGCA 2 cut(s) 416, 1119
NsiI ATGCAT 1 cut(s) 1224
NspI RCATGY 3 cut(s) 389, 820, 1222
NspV TTCGAA 1 cut(s) 174
PagI TCATGA 1 cut(s) 1303
PciI ACATGT 1 cut(s) 816
PctI GAATGC 1 cut(s) 417
PfeI GAWTC 4 cut(s) 157, 302, 1201, 1445
PinAI ACCGGT 1 cut(s) 1396
PkrI GCNGC 1 cut(s) 478
Ple19I CGATCG 1 cut(s) 370
PleI GAGTC 1 cut(s) 1013
PpsI GAGTC 1 cut(s) 1013
PpuMI RGGWCCY 2 cut(s) 163, 916
PscI ACATGT 1 cut(s) 816
PshAI GACNNNNGTC 1 cut(s) 1023
PshBI ATTAAT 2 cut(s) 504, 663
PsiI TTATAA 1 cut(s) 1143
Psp124BI GAGCTC 1 cut(s) 267
Psp5II RGGWCCY 2 cut(s) 163, 916
Psp6I CCWGG 3 cut(s) 89, 693, 988
PspGI CCWGG 3 cut(s) 89, 693, 988
PspN4I GGNNCC 3 cut(s) 917, 918, 1316
PspPI GGNCC 4 cut(s) 163, 674, 916, 1058
PspPPI RGGWCCY 2 cut(s) 163, 916
PvuI CGATCG 1 cut(s) 370
PvuII CAGCTG 1 cut(s) 959
RsaI GTAC 2 cut(s) 56, 982
RsaNI GTAC 2 cut(s) 55, 981
RseI CAYNNNNRTG 3 cut(s) 819, 1339, 1343
SacI GAGCTC 1 cut(s) 267
SalI GTCGAC 1 cut(s) 28
SaqAI TTAA 6 cut(s) 504, 663, 737, 861, 881, 1418
SatI GCNGC 1 cut(s) 477
Sau3AI GATC 3 cut(s) 367, 811, 1291
Sau96I GGNCC 4 cut(s) 163, 674, 916, 1058
SchI GAGTC 1 cut(s) 1013
ScrFI CCNGG 3 cut(s) 91, 695, 990
SduI GDGCHC 3 cut(s) 118, 267, 954
SfaNI GCATC 2 cut(s) 859, 993
SfuI TTCGAA 1 cut(s) 174
SinI GGWCC 4 cut(s) 163, 674, 916, 1058
SmiMI CAYNNNNRTG 3 cut(s) 819, 1339, 1343
SmlI CTYRAG 4 cut(s) 23, 281, 634, 1166
SmoI CTYRAG 4 cut(s) 23, 281, 634, 1166
Sse9I AATT 8 cut(s) 133, 501, 505, 862, 882, 1069, 1145, 1325
SsiI CCGC 3 cut(s) 121, 169, 242
SstI GAGCTC 1 cut(s) 267
StyD4I CCNGG 3 cut(s) 89, 693, 988
StyI CCWWGG 1 cut(s) 10
TaaI ACNGT 3 cut(s) 410, 546, 1310
TaiI ACGT 1 cut(s) 903
TaqI TCGA 5 cut(s) 29, 112, 174, 571, 690
TasI AATT 8 cut(s) 133, 501, 505, 862, 882, 1069, 1145, 1325
TatI WGTACW 2 cut(s) 54, 980
TfiI GAWTC 4 cut(s) 157, 302, 1201, 1445
Tru1I TTAA 6 cut(s) 504, 663, 737, 861, 881, 1418
Tru9I TTAA 6 cut(s) 504, 663, 737, 861, 881, 1418
TscAI CASTG 3 cut(s) 760, 895, 1000
TseFI GTSAC 3 cut(s) 546, 683, 700
TseI GCWGC 1 cut(s) 476
Tsp45I GTSAC 3 cut(s) 546, 683, 700
TspDTI ATGAA 9 cut(s) 572, 656, 735, 777, 1033, 1260, 1292, 1356, 1458
TspRI CASTG 3 cut(s) 760, 895, 1000
VpaK11BI GGWCC 4 cut(s) 163, 674, 916, 1058
VspI ATTAAT 2 cut(s) 504, 663
XagI CCTNNNNNAGG 1 cut(s) 924
XapI RAATTY 2 cut(s) 1145, 1325
XceI RCATGY 3 cut(s) 389, 820, 1222
XmiI GTMKAC 1 cut(s) 29
Zsp2I ATGCAT 1 cut(s) 1224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.