Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0004163

Physical Location & Seq

Genomic Coordinates Reverse (-)

21529731 .. 21532572

Gene Structure 2842 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G58140.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1290 bp

ATGACCGTTTTCTCAGTTCAGTCCACTATCTTCAGTCGAGCCTCCGTAGCTCTTCTCTCGAGCAATGGCTTCAAACGATTTTCATTCGTTTCTTCGTTTTCTTCCTCCGCCGCTTACTCTCCACCTAAAATGAGGAAGCGTCGCTACCCAATCGTCTCTGCTGTTGATATTGGTGGCGTCGCAATCGCTAGAAATGATGTGGTGAGAGAGGATGATCCAACAAATAATGTACCAGATTCGATTTTCTCTAAACTAGGAATGCAGCTACACAGAAGAGATAAGCATCCGATTGGTATCTTAAAAAACGCTATCTACGATTACTTTGATTCCAATTACTCAAACAAGTTTGAGAAGTTCGAAGACCTTTCCCCAATTGTTACCACAAAGCAAAACTTTGATGATGTGCTAGTCCCTGCTGATCATGTAAGCAGAAGTCTTAATGACACGTACTATGTAGACTCACAAACTGTTTTGAGATGTCATACGAGTGCTCACCAAGCTGAGCTGTTGAGGAAAGGTCATAGTCGTTTCCTTGTAACCGGGGATGTTTACCGAAGAGATTCTATTGACTCTACTCATTATCCGGTTTTCCATCAGATGGAAGGTTTTTGTGTTTTCTCTCCTGAGGACTGGAACGGGTCTGGCAAGGATTCCACTTTGTATGCTGCTGAGGATTTGAAGAAATGTCTTGAGGGATTGGCACGCCACTTATTTGGTTCGGTGGAGATGAGATGGGTTGATACATATTTCCCATTTACCAATCCATCTTTTGAGCTTGAGATATATTTTAAGGAAGACTGGTTGGAGGTTTTGGGCTGTGGGGTGACCGAGCAAGTAATTCTGAAACAAAGTGGATTAGAAAATAATGTTGCTTGGGCCTTCGGACTTGGACTTGAGAGACTTGCTATGGTTTTGTTTGACATACCTGATATACGATTTTTCTGGTCATCCGATGAACGATTCACGTCCCAGTTTGGAAAAGGAGAACTTGGAGTGAAATTCAAGCCATATTCAAAGTATCCTCCTTGTTACAAGGACATCAGTTTCTGGATAAGTGATTTGTTCACAGAGAATAATTTTTGTGAAGTTGTTAGAGGAATTGCTGGGGATCTTGTTGAAGAGGTGAAGTTAATTGACCAATTCACCAATAAGAAGAAAGGGCTGACGAGTCATTGTTACAGAATCGTGTTCCGTTCCATGGAGCGGTCTCTTACGGACGAGGAGGTCAATGATCTGCAGAGTAAAGTGCGTGATGAGGTGCAGAAGAAGCTAAATGTCGAATTAAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004826 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006432 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010467 GO:0016020 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

FDX-ACB tRNA-synt_2d

Protein Analysis

429

Amino Acids

49.25

Weight (kDa)

5.99

Isoelectric Point (pI)

41.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2d	PF01409	90 - 325	3.1e-51	tRNA synthetases class II core domain (F)
FDX-ACB	PF03147	338 - 429	5.5e-23	Ferredoxin-fold anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004163)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G58140
fragaria_vesca	FvH4_3g12680 FvH4_3g12680 FvH4_3g12680
malus_domestica	MD05G1248600.v1.1 MD10G1229100.v1.1
prunus_persica	Prupe.4G115500_v2.0.a1
pyrus_communis	pycom05g22530 pycom10g19270
rosa_chinensis	RchiOBHm_Chr3g0486261 RchiOBHm_Chr5g0020641
rosa_laevigata	RLG00000023089 RLG00000032549
rosa_multiflora	Rmu_sc0001857.1_g000018 Rmu_sc0005607.1_g000020 Rmu_sc0005842.1_g000003 Rmu_sc0007661.1_g000010
rosa_roxburghii	Rroxscaffold_1G00057630 Rroxscaffold_6G00395930
rosa_rugosa	Rorug03G0224300 Rorug03G0224400 Rorug03G0224500 Rorug05G0062200
rosa_samantha	Rh3AG274300 Rh3BG309600 Rh3CG308800 Rh5AG151900 Rh5BG151600 Rh5CG162900 Rh5DG150800
rosa_wichuraiana	Rw0G022550 Rw3G024320 Rw5G013460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1223
AccB7I	CCANNNNNTGG	1 cut(s)	598
AccBSI	CCGCTC	1 cut(s)	1204
AccI	GTMKAC	1 cut(s)	456
AciI	CCGC	3 cut(s)	108, 111, 1204
AclWI	GGATC	2 cut(s)	209, 1116
AcsI	RAATTY	1 cut(s)	998
AcuI	CTGAAG	1 cut(s)	16
AcyI	GRCGYC	1 cut(s)	177
AfaI	GTAC	2 cut(s)	231, 449
AfiI	CCNNNNNNNGG	3 cut(s)	598, 1198, 1203
AflIII	ACRYGT	1 cut(s)	444
AgsI	TTSAA	5 cut(s)	73, 679, 1003, 1014, 1118
AjiI	CACGTC	1 cut(s)	966
AluBI	AGCT	6 cut(s)	50, 265, 500, 505, 775, 1270
AluI	AGCT	6 cut(s)	50, 265, 500, 505, 775, 1270
Alw21I	GWGCWC	1 cut(s)	493
Alw26I	GTCTC	3 cut(s)	160, 892, 1212
AlwI	GGATC	2 cut(s)	209, 1116
AlwNI	CAGNNNCTG	1 cut(s)	1047
Ama87I	CYCGRG	1 cut(s)	58
AoxI	GGCC	1 cut(s)	876
ApeKI	GCWGC	2 cut(s)	262, 665
ApoI	RAATTY	1 cut(s)	998
Asp700I	GAANNNNTTC	1 cut(s)	559
AspS9I	GGNCC	1 cut(s)	876
AsuC2I	CCSGG	1 cut(s)	541
AsuHPI	GGTGA	5 cut(s)	214, 485, 835, 1135, 1135
AsuII	TTCGAA	1 cut(s)	357
AvaI	CYCGRG	1 cut(s)	58
AxyI	CCTNAGG	1 cut(s)	624
BarI	GAAGNNNNNNTAC	2 cut(s)	128, 160
BbsI	GAAGAC	2 cut(s)	366, 801
Bbv12I	GWGCWC	1 cut(s)	493
BbvCI	CCTCAGC	1 cut(s)	669
BbvI	GCAGC	2 cut(s)	274, 652
BccI	CCATC	4 cut(s)	592, 600, 726, 772
BciVI	GTATCC	1 cut(s)	1029
BclI	TGATCA	1 cut(s)	418
BcnI	CCSGG	1 cut(s)	541
BcoDI	GTCTC	3 cut(s)	160, 892, 1212
BfaI	CTAG	3 cut(s)	189, 254, 407
BfmI	CTRYAG	1 cut(s)	1235
BfuI	GTATCC	1 cut(s)	1029
BisI	GCNGC	3 cut(s)	111, 263, 666
BlpI	GCTNAGC	1 cut(s)	501
BlsI	GCNGC	3 cut(s)	112, 264, 667
Bme1390I	CCNGG	1 cut(s)	541
BmeT110I	CYCGRG	1 cut(s)	58
BmgBI	CACGTC	1 cut(s)	966
BmgT120I	GGNCC	1 cut(s)	876
BmrFI	CCNGG	1 cut(s)	541
BmrI	ACTGGG	1 cut(s)	964
BmsI	GCATC	1 cut(s)	292
BmuI	ACTGGG	1 cut(s)	964
BpiI	GAAGAC	2 cut(s)	366, 801
Bpu10I	CCTNAGC	1 cut(s)	669
Bpu1102I	GCTNAGC	1 cut(s)	501
Bpu14I	TTCGAA	1 cut(s)	357
BpuEI	CTTGAG	3 cut(s)	710, 797, 914
BpuMI	CCSGG	1 cut(s)	541
BsaAI	YACGTR	1 cut(s)	447
BsaBI	GATNNNNATC	2 cut(s)	282, 293
BsaHI	GRCGYC	1 cut(s)	177
BsaI	GGTCTC	1 cut(s)	1212
BsaJI	CCNNGG	2 cut(s)	540, 1197
BsaWI	WCCGGW	1 cut(s)	583
Bsc4I	CCNNNNNNNGG	3 cut(s)	598, 1198, 1203
Bse1I	ACTGG	3 cut(s)	635, 803, 970
Bse21I	CCTNAGG	1 cut(s)	624
Bse3DI	GCAATG	1 cut(s)	70
Bse8I	GATNNNNATC	2 cut(s)	282, 293
BseDI	CCNNGG	2 cut(s)	540, 1197
BseGI	GGATG	4 cut(s)	217, 283, 550, 947
BseJI	GATNNNNATC	2 cut(s)	282, 293
BseLI	CCNNNNNNNGG	3 cut(s)	598, 1198, 1203
BseMI	GCAATG	1 cut(s)	70
BseMII	CTCAG	4 cut(s)	27, 492, 615, 660
BseNI	ACTGG	3 cut(s)	635, 803, 970
BseRI	GAGGAG	1 cut(s)	1235
BseXI	GCAGC	2 cut(s)	274, 652
BseYI	CCCAGC	1 cut(s)	1103
BsgI	GTGCAG	1 cut(s)	1280
BshFI	GGCC	1 cut(s)	878
BsiHKAI	GWGCWC	1 cut(s)	493
BsiHKCI	CYCGRG	1 cut(s)	58
BsiSI	CCGG	2 cut(s)	540, 584
BslFI	GGGAC	2 cut(s)	395, 952
BslI	CCNNNNNNNGG	3 cut(s)	598, 1198, 1203
BsmAI	GTCTC	3 cut(s)	160, 892, 1212
BsmBI	CGTCTC	1 cut(s)	160
BsmFI	GGGAC	2 cut(s)	395, 952
BsmI	GAATGC	1 cut(s)	264
BsnI	GGCC	1 cut(s)	878
Bso31I	GGTCTC	1 cut(s)	1212
BsoBI	CYCGRG	1 cut(s)	58
Bsp119I	TTCGAA	1 cut(s)	357
Bsp1286I	GDGCHC	1 cut(s)	493
Bsp143I	GATC	4 cut(s)	214, 418, 1108, 1231
Bsp1720I	GCTNAGC	1 cut(s)	501
Bsp19I	CCATGG	1 cut(s)	1197
BspACI	CCGC	3 cut(s)	108, 111, 1204
BspANI	GGCC	1 cut(s)	878
BspCNI	CTCAG	4 cut(s)	26, 493, 616, 661
BspMAI	CTGCAG	1 cut(s)	1239
BspPI	GGATC	2 cut(s)	209, 1116
BspQI	GCTCTTC	1 cut(s)	57
BspT104I	TTCGAA	1 cut(s)	357
BspTNI	GGTCTC	1 cut(s)	1212
BsrBI	CCGCTC	1 cut(s)	1204
BsrDI	GCAATG	1 cut(s)	70
BsrI	ACTGG	3 cut(s)	635, 803, 970
BssECI	CCNNGG	2 cut(s)	540, 1197
BssMI	GATC	4 cut(s)	214, 418, 1108, 1231
BssNI	GRCGYC	1 cut(s)	177
BssT1I	CCWWGG	1 cut(s)	1197
Bst4CI	ACNGT	2 cut(s)	7, 469
Bst6I	CTCTTC	4 cut(s)	57, 268, 550, 1113
BstACI	GRCGYC	1 cut(s)	177
BstBAI	YACGTR	1 cut(s)	447
BstBI	TTCGAA	1 cut(s)	357
BstC8I	GCNNGC	1 cut(s)	703
BstDEI	CTNAG	4 cut(s)	13, 501, 624, 669
BstDSI	CCRYGG	1 cut(s)	1197
BstEII	GGTNACC	1 cut(s)	823
BstF5I	GGATG	4 cut(s)	217, 283, 550, 947
BstKTI	GATC	4 cut(s)	217, 421, 1111, 1234
BstMAI	GTCTC	3 cut(s)	160, 892, 1212
BstMBI	GATC	4 cut(s)	214, 418, 1108, 1231
BstMWI	GCNNNNNNNGC	3 cut(s)	47, 497, 1267
BstPI	GGTNACC	1 cut(s)	823
BstSCI	CCNGG	1 cut(s)	539
BstSFI	CTRYAG	1 cut(s)	1235
BstV1I	GCAGC	2 cut(s)	274, 652
BstV2I	GAAGAC	2 cut(s)	366, 801
BstX2I	RGATCY	1 cut(s)	1108
BstXI	CCANNNNNNTGG	1 cut(s)	713
BstYI	RGATCY	1 cut(s)	1108
Bsu36I	CCTNAGG	1 cut(s)	624
BsuI	GTATCC	1 cut(s)	1029
BsuRI	GGCC	1 cut(s)	878
BtgI	CCRYGG	1 cut(s)	1197
BtrI	CACGTC	1 cut(s)	966
BtsCI	GGATG	4 cut(s)	217, 283, 550, 947
Cac8I	GCNNGC	1 cut(s)	703
CaiI	CAGNNNCTG	1 cut(s)	1047
Cfr13I	GGNCC	1 cut(s)	876
CseI	GACGC	2 cut(s)	128, 166
Csp6I	GTAC	2 cut(s)	230, 448
CviAII	CATG	2 cut(s)	422, 1198
CviQI	GTAC	2 cut(s)	230, 448
DdeI	CTNAG	4 cut(s)	13, 501, 624, 669
DpnI	GATC	4 cut(s)	216, 420, 1110, 1233
DpnII	GATC	4 cut(s)	214, 418, 1108, 1231
DrdI	GACNNNNNNGTC	1 cut(s)	1223
DseDI	GACNNNNNNGTC	1 cut(s)	1223
Eam1104I	CTCTTC	4 cut(s)	57, 268, 550, 1113
EarI	CTCTTC	4 cut(s)	57, 268, 550, 1113
EciI	GGCGGA	1 cut(s)	97
Eco130I	CCWWGG	1 cut(s)	1197
Eco31I	GGTCTC	1 cut(s)	1212
Eco57I	CTGAAG	1 cut(s)	16
Eco81I	CCTNAGG	1 cut(s)	624
Eco88I	CYCGRG	1 cut(s)	58
Eco91I	GGTNACC	1 cut(s)	823
EcoO65I	GGTNACC	1 cut(s)	823
EcoT14I	CCWWGG	1 cut(s)	1197
ErhI	CCWWGG	1 cut(s)	1197
Esp3I	CGTCTC	1 cut(s)	160
FaeI	CATG	2 cut(s)	425, 1201
FalI	AAGNNNNNCTT	4 cut(s)	377, 409, 972, 1004
FaqI	GGGAC	2 cut(s)	395, 952
FatI	CATG	2 cut(s)	421, 1197
FbaI	TGATCA	1 cut(s)	418
FblI	GTMKAC	1 cut(s)	456
Fnu4HI	GCNGC	3 cut(s)	111, 263, 666
FokI	GGATG	4 cut(s)	224, 270, 557, 934
Fsp4HI	GCNGC	3 cut(s)	111, 263, 666
FspBI	CTAG	3 cut(s)	189, 254, 407
GluI	GCNGC	3 cut(s)	111, 263, 666
GsaI	CCCAGC	1 cut(s)	1107
HaeIII	GGCC	1 cut(s)	878
HapII	CCGG	2 cut(s)	540, 584
HgaI	GACGC	2 cut(s)	128, 166
Hin1I	GRCGYC	1 cut(s)	177
Hin1II	CATG	2 cut(s)	425, 1201
HinfI	GANTC	9 cut(s)	236, 326, 458, 560, 569, 650, 960, 1168, 1182
HpaII	CCGG	2 cut(s)	540, 584
HphI	GGTGA	5 cut(s)	214, 485, 835, 1135, 1135
Hpy166II	GTNNAC	4 cut(s)	24, 457, 550, 1065
Hpy188I	TCNGA	5 cut(s)	288, 597, 843, 884, 952
Hpy188III	TCNNGA	4 cut(s)	58, 623, 689, 1048
Hpy8I	GTNNAC	4 cut(s)	24, 457, 550, 1065
Hpy99I	CGWCG	2 cut(s)	144, 182
HpyAV	CCTTC	2 cut(s)	596, 889
HpyCH4III	ACNGT	2 cut(s)	7, 469
HpyCH4IV	ACGT	2 cut(s)	446, 965
HpyCH4V	TGCA	3 cut(s)	262, 1237, 1261
HpyF10VI	GCNNNNNNNGC	3 cut(s)	47, 497, 1267
HpyF3I	CTNAG	4 cut(s)	13, 501, 624, 669
HpySE526I	ACGT	2 cut(s)	446, 965
Hsp92I	GRCGYC	1 cut(s)	177
Hsp92II	CATG	2 cut(s)	425, 1201
Ksp22I	TGATCA	1 cut(s)	418
Kzo9I	GATC	4 cut(s)	214, 418, 1108, 1231
LguI	GCTCTTC	1 cut(s)	57
LmnI	GCTCC	1 cut(s)	1201
Lsp1109I	GCAGC	2 cut(s)	274, 652
LweI	GCATC	1 cut(s)	292
MaeI	CTAG	3 cut(s)	189, 254, 407
MaeII	ACGT	2 cut(s)	446, 965
MaeIII	GTNAC	5 cut(s)	376, 535, 823, 1028, 1175
MalI	GATC	4 cut(s)	216, 420, 1110, 1233
MbiI	CCGCTC	1 cut(s)	1204
MboI	GATC	4 cut(s)	214, 418, 1108, 1231
MfeI	CAATTG	1 cut(s)	372
MflI	RGATCY	1 cut(s)	1108
MhlI	GDGCHC	1 cut(s)	493
MluCI	AATT	9 cut(s)	331, 372, 837, 998, 1075, 1098, 1131, 1139, 1280
MlyI	GAGTC	3 cut(s)	452, 563, 1177
MmeI	TCCRAC	2 cut(s)	242, 783
MroXI	GAANNNNTTC	1 cut(s)	559
MseI	TTAA	5 cut(s)	299, 438, 789, 1130, 1283
MslI	CAYNNNNRTG	1 cut(s)	486
MspI	CCGG	2 cut(s)	540, 584
MspR9I	CCNGG	1 cut(s)	541
MunI	CAATTG	1 cut(s)	372
Mva1269I	GAATGC	1 cut(s)	264
MwoI	GCNNNNNNNGC	3 cut(s)	47, 497, 1267
NciI	CCSGG	1 cut(s)	541
NcoI	CCATGG	1 cut(s)	1197
NdeII	GATC	4 cut(s)	214, 418, 1108, 1231
NlaIII	CATG	2 cut(s)	425, 1201
NmuCI	GTSAC	1 cut(s)	823
NspV	TTCGAA	1 cut(s)	357
PaeR7I	CTCGAG	1 cut(s)	58
PciSI	GCTCTTC	1 cut(s)	57
PcsI	WCGNNNNNNNCGW	1 cut(s)	312
PctI	GAATGC	1 cut(s)	264
PdmI	GAANNNNTTC	1 cut(s)	559
PfeI	GAWTC	6 cut(s)	236, 326, 560, 650, 960, 1182
PflMI	CCANNNNNTGG	1 cut(s)	598
PkrI	GCNGC	3 cut(s)	112, 264, 667
PleI	GAGTC	3 cut(s)	452, 563, 1176
PpsI	GAGTC	3 cut(s)	452, 563, 1176
Ppu21I	YACGTR	1 cut(s)	447
PspEI	GGTNACC	1 cut(s)	823
PspFI	CCCAGC	1 cut(s)	1103
PspPI	GGNCC	1 cut(s)	876
PstI	CTGCAG	1 cut(s)	1239
PstNI	CAGNNNCTG	1 cut(s)	1047
PsuI	RGATCY	1 cut(s)	1108
RsaI	GTAC	2 cut(s)	231, 449
RsaNI	GTAC	2 cut(s)	230, 448
RseI	CAYNNNNRTG	1 cut(s)	486
SapI	GCTCTTC	1 cut(s)	57
SaqAI	TTAA	5 cut(s)	299, 438, 789, 1130, 1283
SatI	GCNGC	3 cut(s)	111, 263, 666
Sau3AI	GATC	4 cut(s)	214, 418, 1108, 1231
Sau96I	GGNCC	1 cut(s)	876
SchI	GAGTC	3 cut(s)	452, 563, 1177
ScrFI	CCNGG	1 cut(s)	541
SduI	GDGCHC	1 cut(s)	493
SfaNI	GCATC	1 cut(s)	292
SfcI	CTRYAG	1 cut(s)	1235
Sfr274I	CTCGAG	1 cut(s)	58
SfuI	TTCGAA	1 cut(s)	357
SlaI	CTCGAG	1 cut(s)	58
SmiMI	CAYNNNNRTG	1 cut(s)	486
SmlI	CTYRAG	4 cut(s)	58, 689, 776, 893
SmoI	CTYRAG	4 cut(s)	58, 689, 776, 893
Sse9I	AATT	9 cut(s)	331, 372, 837, 998, 1075, 1098, 1131, 1139, 1280
SsiI	CCGC	3 cut(s)	108, 111, 1204
SspMI	CTAG	3 cut(s)	189, 254, 407
StyD4I	CCNGG	1 cut(s)	539
StyI	CCWWGG	1 cut(s)	1197
TaaI	ACNGT	2 cut(s)	7, 469
TaiI	ACGT	2 cut(s)	449, 968
TaqI	TCGA	5 cut(s)	37, 59, 239, 357, 1278
TaqII	GACCGA	1 cut(s)	842
TasI	AATT	9 cut(s)	331, 372, 837, 998, 1075, 1098, 1131, 1139, 1280
TauI	GCSGC	1 cut(s)	113
TfiI	GAWTC	6 cut(s)	236, 326, 560, 650, 960, 1182
Tru1I	TTAA	5 cut(s)	299, 438, 789, 1130, 1283
Tru9I	TTAA	5 cut(s)	299, 438, 789, 1130, 1283
TseFI	GTSAC	1 cut(s)	823
TseI	GCWGC	2 cut(s)	262, 665
Tsp45I	GTSAC	1 cut(s)	823
TspDTI	ATGAA	2 cut(s)	72, 969
TspGWI	ACGGA	3 cut(s)	34, 1181, 1229
Van91I	CCANNNNNTGG	1 cut(s)	598
XapI	RAATTY	1 cut(s)	998
XhoI	CTCGAG	1 cut(s)	58
XmiI	GTMKAC	1 cut(s)	456
XmnI	GAANNNNTTC	1 cut(s)	559
XspI	CTAG	3 cut(s)	189, 254, 407

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.