Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0004163

Physical Location & Seq

Genomic Coordinates Reverse (-)

32609822 .. 32614477

Gene Structure 4656 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1229100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1296 bp

ATGGCAACGGCCCTCTCATTTGCGCAGACCACTCTCTTCTCCAAAACCTCACCTTCTCTCCGCACAGCTTGCCTCAGAAGCTTCACTTTCTGCCTGCCCTTCTCTTCTTCCTCTACAGCTCTTTCCTCCGATAAGCTTCACAACAAGAAATGGAGGCAGCCGGTGGCTTCCGTGCTCGAGCTGGGCGGCGTCAAGATTGCTAAAGATGATGTGCTGAGGGATGACCCCACAAACAATGTACCGGATACAATTTTTGCAAAGCTTGGACTGCAACTCCACAGAAGAGATCAGCACCCACTTGGGATTTTGAAGAATGCAATATATGAGTATTTCGACACCAATTACTCAAATCAGTTCGATAAGTTTGATAATCTTTGCCCAATTGTCTCTGTGAAAGAGAATTTTGACGATGTTTTGGTTCCTGCTGATCATGTAAGTCGAAGTTATAATGATACGTACTATATTGACTCTCAAACCGTTTTGAGGTGTCATACAAGTGCTCATCAGGCAGAAATATTGAGAAGAGGACATACTCATTTCCTTGTAACAGGAGATGTGTACCGTAGGGATTCCATTGACTCAACACATTACCCTGTGTTCCATCAGATGGAAGGTGTCCGTGTTTTTTCTCCACAAGATTGGGAGGCTTCTGGGACAGATGGCACATCTTATGCTGCTGGAGATTTAAAGAAATGTCTAGAGGGATTGGCACGCCACTTATTTGGTTCTGTGGAAATGCGCTGGGTTGATACATATTTCCCATTTACCAACCCATCATTTGAACTTGAGATATTTTTTCAGGAAAAATGGTTGGAGGTTTTGGGTTGTGGGGTGACAGAACAAGAAATATTGAGGAGAAGTGGCAAAACAGACAACGTTGCTTGGGCTTTTGGACTTGGACTGGAACGGCTTGCAATGGTTTTATTTGACATCCCTGATATTCGGCTTTTCTGGTCAACTGATGAGAGATTCACCTCTCAGTTTTCAAGTGGCCAGCTGGGAATCAAATTCAAGCCATTTTCCAAGTATCCTCCTTGTTACAAAGATGTCAGTTTTTGGATAAACGAGTCTTTCACTGAAAACAATTTATGTGAAGTTGTTAGAGAAGTTGCTGGAGATCTTGCAGAGGAGGTGCAATTGATAGACAATTTCACAAACAAGAAAGGGATGACCAGTCACTGCTACAGAATTGCATATCGATCCATGGAACGCTCGCTCACAGATGATGAAATTAATAAGTTGCAGTGGAATGTAAGAGAGTTGGTGCAGAGCAAGTTGAACGTCGTTCTAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004826 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006432 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0010467 GO:0016020 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

FDX-ACB tRNA-synt_2d

Protein Analysis

432

Amino Acids

49.43

Weight (kDa)

5.88

Isoelectric Point (pI)

46.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2d	PF01409	116 - 328	8.1e-49	tRNA synthetases class II core domain (F)
FDX-ACB	PF03147	341 - 431	3.5e-21	Ferredoxin-fold anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004163)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G58140
fragaria_vesca	FvH4_3g12680 FvH4_3g12680 FvH4_3g12680
malus_domestica	MD05G1248600.v1.1 MD10G1229100.v1.1
prunus_persica	Prupe.4G115500_v2.0.a1
pyrus_communis	pycom05g22530 pycom10g19270
rosa_chinensis	RchiOBHm_Chr3g0486261 RchiOBHm_Chr5g0020641
rosa_laevigata	RLG00000023089 RLG00000032549
rosa_multiflora	Rmu_sc0001857.1_g000018 Rmu_sc0005607.1_g000020 Rmu_sc0005842.1_g000003 Rmu_sc0007661.1_g000010
rosa_roxburghii	Rroxscaffold_1G00057630 Rroxscaffold_6G00395930
rosa_rugosa	Rorug03G0224300 Rorug03G0224400 Rorug03G0224500 Rorug05G0062200
rosa_samantha	Rh3AG274300 Rh3BG309600 Rh3CG308800 Rh5AG151900 Rh5BG151600 Rh5CG162900 Rh5DG150800
rosa_wichuraiana	Rw0G022550 Rw3G024320 Rw5G013460

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	447
Acc16I	TGCGCA	1 cut(s)	24
AccB7I	CCANNNNNTGG	1 cut(s)	607
AciI	CCGC	2 cut(s)	61, 186
AclI	AACGTT	1 cut(s)	876
AclWI	GGATC	1 cut(s)	1194
AcoI	YGGCCR	1 cut(s)	991
AcsI	RAATTY	2 cut(s)	400, 1007
AcyI	GRCGYC	1 cut(s)	189
AfaI	GTAC	3 cut(s)	240, 458, 560
AfiI	CCNNNNNNNGG	2 cut(s)	483, 607
AgsI	TTSAA	5 cut(s)	310, 782, 987, 1012, 1279
AluBI	AGCT	7 cut(s)	68, 81, 119, 136, 181, 262, 997
AluI	AGCT	7 cut(s)	68, 81, 119, 136, 181, 262, 997
Alw21I	GWGCWC	2 cut(s)	177, 502
Alw26I	GTCTC	1 cut(s)	391
AlwI	GGATC	1 cut(s)	1194
AlwNI	CAGNNNCTG	1 cut(s)	1179
Ama87I	CYCGRG	1 cut(s)	176
AoxI	GGCC	2 cut(s)	9, 991
ApeKI	GCWGC	2 cut(s)	157, 674
ApoI	RAATTY	2 cut(s)	400, 1007
AseI	ATTAAT	1 cut(s)	1233
AspLEI	GCGC	2 cut(s)	25, 741
AspS9I	GGNCC	1 cut(s)	10
AsuHPI	GGTGA	3 cut(s)	42, 844, 964
AvaI	CYCGRG	1 cut(s)	176
BalI	TGGCCA	1 cut(s)	993
BarI	GAAGNNNNNNTAC	2 cut(s)	514, 546
Bbv12I	GWGCWC	2 cut(s)	177, 502
BbvCI	CCTCAGC	1 cut(s)	215
BbvI	GCAGC	2 cut(s)	169, 661
BccI	CCATC	4 cut(s)	601, 609, 653, 781
BceAI	ACGGC	2 cut(s)	24, 923
BciVI	GTATCC	2 cut(s)	238, 1038
BclI	TGATCA	1 cut(s)	427
BcoDI	GTCTC	1 cut(s)	391
BfaI	CTAG	1 cut(s)	698
BfmI	CTRYAG	2 cut(s)	114, 1183
BfuI	GTATCC	2 cut(s)	238, 1038
BglII	AGATCT	1 cut(s)	1117
BisI	GCNGC	3 cut(s)	158, 187, 675
BlsI	GCNGC	3 cut(s)	159, 188, 676
BmeT110I	CYCGRG	1 cut(s)	176
BmgT120I	GGNCC	1 cut(s)	10
BmiI	GGNNCC	1 cut(s)	420
BpmI	CTGGAG	2 cut(s)	699, 1134
Bpu10I	CCTNAGC	1 cut(s)	215
BpuEI	CTTGAG	1 cut(s)	806
Bsa29I	ATCGAT	1 cut(s)	1198
BsaAI	YACGTR	1 cut(s)	456
BsaHI	GRCGYC	1 cut(s)	189
BsaJI	CCNNGG	1 cut(s)	1203
BsaWI	WCCGGW	1 cut(s)	241
BsaXI	ACNNNNNCTCC	4 cut(s)	42, 72, 258, 288
Bsc4I	CCNNNNNNNGG	2 cut(s)	483, 607
Bse118I	RCCGGY	1 cut(s)	160
Bse1I	ACTGG	2 cut(s)	906, 1173
Bse3DI	GCAATG	1 cut(s)	921
BseCI	ATCGAT	1 cut(s)	1198
BseDI	CCNNGG	1 cut(s)	1203
BseGI	GGATG	3 cut(s)	226, 930, 1173
BseLI	CCNNNNNNNGG	2 cut(s)	483, 607
BseMI	GCAATG	1 cut(s)	921
BseMII	CTCAG	3 cut(s)	88, 206, 992
BseNI	ACTGG	2 cut(s)	906, 1173
BseRI	GAGGAG	2 cut(s)	868, 1142
BseXI	GCAGC	2 cut(s)	169, 661
BseYI	CCCAGC	3 cut(s)	181, 741, 997
BsgI	GTGCAG	1 cut(s)	1286
BshFI	GGCC	2 cut(s)	11, 993
BshVI	ATCGAT	1 cut(s)	1198
BsiHKAI	GWGCWC	2 cut(s)	177, 502
BsiHKCI	CYCGRG	1 cut(s)	176
BsiSI	CCGG	2 cut(s)	161, 242
BslFI	GGGAC	1 cut(s)	667
BslI	CCNNNNNNNGG	2 cut(s)	483, 607
BsmAI	GTCTC	1 cut(s)	391
BsmFI	GGGAC	1 cut(s)	667
BsmI	GAATGC	1 cut(s)	319
BsnI	GGCC	2 cut(s)	11, 993
BsoBI	CYCGRG	1 cut(s)	176
Bsp1286I	GDGCHC	2 cut(s)	177, 502
Bsp143I	GATC	4 cut(s)	286, 427, 1117, 1199
Bsp19I	CCATGG	1 cut(s)	1203
BspACI	CCGC	2 cut(s)	61, 186
BspANI	GGCC	2 cut(s)	11, 993
BspCNI	CTCAG	3 cut(s)	87, 207, 991
BspDI	ATCGAT	1 cut(s)	1198
BspLI	GGNNCC	1 cut(s)	420
BspPI	GGATC	1 cut(s)	1194
BsrDI	GCAATG	1 cut(s)	921
BsrFI	RCCGGY	1 cut(s)	160
BsrI	ACTGG	2 cut(s)	906, 1173
BssAI	RCCGGY	1 cut(s)	160
BssECI	CCNNGG	1 cut(s)	1203
BssMI	GATC	4 cut(s)	286, 427, 1117, 1199
BssNI	GRCGYC	1 cut(s)	189
BssT1I	CCWWGG	1 cut(s)	1203
Bst4CI	ACNGT	2 cut(s)	478, 563
Bst6I	CTCTTC	4 cut(s)	41, 109, 277, 517
BstACI	GRCGYC	1 cut(s)	189
BstAPI	GCANNNNNTGC	1 cut(s)	69
BstBAI	YACGTR	1 cut(s)	456
BstC8I	GCNNGC	6 cut(s)	70, 95, 712, 912, 995, 1214
BstDEI	CTNAG	4 cut(s)	74, 215, 978, 1289
BstDSI	CCRYGG	1 cut(s)	1203
BstF5I	GGATG	3 cut(s)	226, 930, 1173
BstHHI	GCGC	2 cut(s)	25, 741
BstKTI	GATC	4 cut(s)	289, 430, 1120, 1202
BstMAI	GTCTC	1 cut(s)	391
BstMBI	GATC	4 cut(s)	286, 427, 1117, 1199
BstMWI	GCNNNNNNNGC	4 cut(s)	69, 78, 268, 506
BstSFI	CTRYAG	2 cut(s)	114, 1183
BstSNI	TACGTA	1 cut(s)	456
BstV1I	GCAGC	2 cut(s)	169, 661
BstX2I	RGATCY	1 cut(s)	1117
BstXI	CCANNNNNNTGG	2 cut(s)	639, 722
BstYI	RGATCY	1 cut(s)	1117
Bsu15I	ATCGAT	1 cut(s)	1198
BsuI	GTATCC	2 cut(s)	238, 1038
BsuRI	GGCC	2 cut(s)	11, 993
BsuTUI	ATCGAT	1 cut(s)	1198
BtgI	CCRYGG	1 cut(s)	1203
BtsCI	GGATG	3 cut(s)	226, 930, 1173
BtsI	GCAGTG	2 cut(s)	1177, 1250
BtsIMutI	CAGTG	3 cut(s)	1074, 1177, 1250
Cac8I	GCNNGC	6 cut(s)	70, 95, 712, 912, 995, 1214
CaiI	CAGNNNCTG	1 cut(s)	1179
CfoI	GCGC	2 cut(s)	25, 741
Cfr10I	RCCGGY	1 cut(s)	160
Cfr13I	GGNCC	1 cut(s)	10
ClaI	ATCGAT	1 cut(s)	1198
CseI	GACGC	1 cut(s)	178
Csp6I	GTAC	3 cut(s)	239, 457, 559
CviAII	CATG	2 cut(s)	431, 1204
CviQI	GTAC	3 cut(s)	239, 457, 559
DdeI	CTNAG	4 cut(s)	74, 215, 978, 1289
DpnI	GATC	4 cut(s)	288, 429, 1119, 1201
DpnII	GATC	4 cut(s)	286, 427, 1117, 1199
DraI	TTTAAA	1 cut(s)	687
EaeI	YGGCCR	1 cut(s)	991
Eam1104I	CTCTTC	4 cut(s)	41, 109, 277, 517
EarI	CTCTTC	4 cut(s)	41, 109, 277, 517
Eco105I	TACGTA	1 cut(s)	456
Eco130I	CCWWGG	1 cut(s)	1203
Eco88I	CYCGRG	1 cut(s)	176
EcoT14I	CCWWGG	1 cut(s)	1203
ErhI	CCWWGG	1 cut(s)	1203
FaeI	CATG	2 cut(s)	434, 1207
FalI	AAGNNNNNCTT	2 cut(s)	70, 102
FaqI	GGGAC	1 cut(s)	667
FatI	CATG	2 cut(s)	430, 1203
FbaI	TGATCA	1 cut(s)	427
Fnu4HI	GCNGC	3 cut(s)	158, 187, 675
FokI	GGATG	3 cut(s)	233, 917, 1180
Fsp4HI	GCNGC	3 cut(s)	158, 187, 675
FspBI	CTAG	1 cut(s)	698
FspI	TGCGCA	1 cut(s)	24
GlaI	GCGC	2 cut(s)	24, 740
GluI	GCNGC	3 cut(s)	158, 187, 675
GsaI	CCCAGC	3 cut(s)	185, 745, 1001
GsuI	CTGGAG	2 cut(s)	699, 1134
HaeIII	GGCC	2 cut(s)	11, 993
HapII	CCGG	2 cut(s)	161, 242
HgaI	GACGC	1 cut(s)	178
HhaI	GCGC	2 cut(s)	25, 741
Hin1I	GRCGYC	1 cut(s)	189
Hin1II	CATG	2 cut(s)	434, 1207
Hin6I	GCGC	2 cut(s)	23, 739
HinP1I	GCGC	2 cut(s)	23, 739
HincII	GTYRAC	1 cut(s)	957
HindII	GTYRAC	1 cut(s)	957
HindIII	AAGCTT	3 cut(s)	79, 134, 260
HinfI	GANTC	6 cut(s)	467, 569, 578, 969, 1002, 1067
HpaII	CCGG	2 cut(s)	161, 242
HphI	GGTGA	3 cut(s)	42, 844, 964
Hpy166II	GTNNAC	2 cut(s)	559, 957
Hpy188I	TCNGA	3 cut(s)	77, 130, 606
Hpy188III	TCNNGA	3 cut(s)	193, 698, 800
Hpy8I	GTNNAC	2 cut(s)	559, 957
Hpy99I	CGWCG	1 cut(s)	1286
HpyAV	CCTTC	3 cut(s)	63, 109, 605
HpyCH4III	ACNGT	2 cut(s)	478, 563
HpyCH4IV	ACGT	3 cut(s)	455, 876, 1281
HpyCH4V	TGCA	9 cut(s)	257, 271, 317, 914, 1124, 1135, 1193, 1243, 1267
HpyF10VI	GCNNNNNNNGC	4 cut(s)	69, 78, 268, 506
HpyF3I	CTNAG	4 cut(s)	74, 215, 978, 1289
HpySE526I	ACGT	3 cut(s)	455, 876, 1281
Hsp92I	GRCGYC	1 cut(s)	189
Hsp92II	CATG	2 cut(s)	434, 1207
HspAI	GCGC	2 cut(s)	23, 739
Ksp22I	TGATCA	1 cut(s)	427
Kzo9I	GATC	4 cut(s)	286, 427, 1117, 1199
Lsp1109I	GCAGC	2 cut(s)	169, 661
MaeI	CTAG	1 cut(s)	698
MaeII	ACGT	3 cut(s)	455, 876, 1281
MaeIII	GTNAC	4 cut(s)	544, 832, 1037, 1175
MalI	GATC	4 cut(s)	288, 429, 1119, 1201
MboI	GATC	4 cut(s)	286, 427, 1117, 1199
MboII	GAAGA	6 cut(s)	28, 96, 99, 294, 322, 534
MfeI	CAATTG	2 cut(s)	381, 1136
MflI	RGATCY	1 cut(s)	1117
MhlI	GDGCHC	2 cut(s)	177, 502
MlsI	TGGCCA	1 cut(s)	993
MluNI	TGGCCA	1 cut(s)	993
MlyI	GAGTC	3 cut(s)	461, 572, 1076
MmeI	TCCRAC	1 cut(s)	792
Mox20I	TGGCCA	1 cut(s)	993
MscI	TGGCCA	1 cut(s)	993
MseI	TTAA	2 cut(s)	686, 1233
MslI	CAYNNNNRTG	1 cut(s)	495
Msp20I	TGGCCA	1 cut(s)	993
MspA1I	CMGCKG	1 cut(s)	997
MspI	CCGG	2 cut(s)	161, 242
MunI	CAATTG	2 cut(s)	381, 1136
Mva1269I	GAATGC	1 cut(s)	319
MwoI	GCNNNNNNNGC	4 cut(s)	69, 78, 268, 506
NcoI	CCATGG	1 cut(s)	1203
NdeII	GATC	4 cut(s)	286, 427, 1117, 1199
NlaIII	CATG	2 cut(s)	434, 1207
NlaIV	GGNNCC	1 cut(s)	420
NmuCI	GTSAC	2 cut(s)	832, 1175
NsbI	TGCGCA	1 cut(s)	24
PaeR7I	CTCGAG	1 cut(s)	176
PctI	GAATGC	1 cut(s)	319
PfeI	GAWTC	3 cut(s)	569, 969, 1002
PflMI	CCANNNNNTGG	1 cut(s)	607
PkrI	GCNGC	3 cut(s)	159, 188, 676
PleI	GAGTC	3 cut(s)	461, 572, 1075
PpsI	GAGTC	3 cut(s)	461, 572, 1075
Ppu21I	YACGTR	1 cut(s)	456
PshBI	ATTAAT	1 cut(s)	1233
PsiI	TTATAA	1 cut(s)	447
Psp1406I	AACGTT	1 cut(s)	876
PspFI	CCCAGC	3 cut(s)	181, 741, 997
PspN4I	GGNNCC	1 cut(s)	420
PspPI	GGNCC	1 cut(s)	10
PspXI	VCTCGAGB	1 cut(s)	176
PstNI	CAGNNNCTG	1 cut(s)	1179
PsuI	RGATCY	1 cut(s)	1117
PvuII	CAGCTG	1 cut(s)	997
RsaI	GTAC	3 cut(s)	240, 458, 560
RsaNI	GTAC	3 cut(s)	239, 457, 559
RseI	CAYNNNNRTG	1 cut(s)	495
SaqAI	TTAA	2 cut(s)	686, 1233
SatI	GCNGC	3 cut(s)	158, 187, 675
Sau3AI	GATC	4 cut(s)	286, 427, 1117, 1199
Sau96I	GGNCC	1 cut(s)	10
SchI	GAGTC	3 cut(s)	461, 572, 1076
SduI	GDGCHC	2 cut(s)	177, 502
SfcI	CTRYAG	2 cut(s)	114, 1183
Sfr274I	CTCGAG	1 cut(s)	176
SlaI	CTCGAG	1 cut(s)	176
SmiMI	CAYNNNNRTG	1 cut(s)	495
SmlI	CTYRAG	2 cut(s)	176, 785
SmoI	CTYRAG	2 cut(s)	176, 785
SnaBI	TACGTA	1 cut(s)	456
SsiI	CCGC	2 cut(s)	61, 186
SspI	AATATT	2 cut(s)	516, 849
SspMI	CTAG	1 cut(s)	698
StyI	CCWWGG	1 cut(s)	1203
TaaI	ACNGT	2 cut(s)	478, 563
TaiI	ACGT	3 cut(s)	458, 879, 1284
TaqI	TCGA	5 cut(s)	177, 333, 357, 439, 1198
TauI	GCSGC	1 cut(s)	189
TfiI	GAWTC	3 cut(s)	569, 969, 1002
Tru1I	TTAA	2 cut(s)	686, 1233
Tru9I	TTAA	2 cut(s)	686, 1233
TscAI	CASTG	3 cut(s)	1081, 1184, 1250
TseFI	GTSAC	2 cut(s)	832, 1175
TseI	GCWGC	2 cut(s)	157, 674
Tsp45I	GTSAC	2 cut(s)	832, 1175
TspDTI	ATGAA	1 cut(s)	1242
TspGWI	ACGGA	2 cut(s)	160, 608
TspRI	CASTG	3 cut(s)	1081, 1184, 1250
Van91I	CCANNNNNTGG	1 cut(s)	607
VspI	ATTAAT	1 cut(s)	1233
XapI	RAATTY	2 cut(s)	400, 1007
XbaI	TCTAGA	1 cut(s)	697
XhoI	CTCGAG	1 cut(s)	176
XspI	CTAG	1 cut(s)	698

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.