AT3G59980

Aminoacyl tRNA synthase complex-interacting multifunctional protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014048
Physical Location & Seq
Reverse (-)
22154244 .. 22155641
1398 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G59980.1

Sequence Viewer

Length: 822 bp
ATGGCGGCGACGATAGCTGTTTTTACCGGTGGTCTGAAAGTGAAGGCTGGAGTTCTCGCCGTTCAAATCTCATCGCTTCCTCTCCGCCGCGAATTCCCTCGTTACTTGTCGTCTTCCTCGAGGAGGACAGTAGCGGCAATCTCCTCGTCTCTTCGAGTCTCCGCCGTATATAACCATCAGCAGCGTAATCGAAGCTCTTGGCCTCGAGTTGCTACTTTCTGTACGGCGGCTCCAGATGCCGGCACGACGGTTTCAGCGGATGAATCGGAGAAGAAGAGCGAATCTCAGAAGGAAGAGGAGAATGTGAAGGAGACGGCGAATCTGTTAGATATAAAAGTAGGACGAATTGTGAAAGCTTGGCAACACGAAGAAGCTGATTCTCTGTATGTTGAAGAAGTCGACATCGGTGAAGCTGAGCCCAGAATCATCTGCAGTGGTCTCGTCAAATATGTTCCTCTTGATCTCCTTCAGGGTGCATCAGTGGTGGTGCTGGCTAATCTGAAGCCGAGGAATATGAGAGGGGTTAAGTCATGTGGTATGCTTTTAGCTGCATCTGACGCAGCTCATGAAAATGTTGAGCTTCTTGTTCCACCAGAAGGCTCTGTTCCTGGAGATAGAGTTTGGTTTGGTAACGAAGAAGATCTCGAGCAGCTTCCTGAACCTGCACCTCCTAATAAGGTTCAAAAGAAGAAGATGTGGGAATTGGTGCAACCACTTTTGAAGACAGATGCGTCTGGTGTTTCAATGCTTAAGGAGCATTTGATGCGGACATCTTCTGGTCTTGTTACTTCCAAGTCTTTAAGAAATGCCAACATTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_bind

Protein Analysis

273

Amino Acids

29.74

Weight (kDa)

7.7

Isoelectric Point (pI)

47.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_bind PF01588 111 - 205 2.4e-29 Putative tRNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014048)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59980
fragaria_vesca FvH4_1g00030 FvH4_1g00030 FvH4_1g00030
malus_domestica MD03G1140600.v1.1 MD11G1171300.v1.1
prunus_persica Prupe.6G126300_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0084251
rosa_laevigata RLG00000015603
rosa_multiflora Rmu_sc0003129.1_g000017
rosa_roxburghii Rroxscaffold_2G00156210
rosa_rugosa Rorug01G0452100
rosa_samantha Rh2AG000500 Rh2BG000400 Rh2CG000300 Rh2DG000500
rosa_wichuraiana Rw2G000030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 730
AbsI CCTCGAGG 1 cut(s) 118
Acc36I ACCTGC 1 cut(s) 670
AccI GTMKAC 1 cut(s) 399
AccII CGCG 1 cut(s) 90
AciI CCGC 8 cut(s) 5, 85, 88, 134, 162, 227, 257, 766
AcsI RAATTY 1 cut(s) 92
AcuI CTGAAG 2 cut(s) 452, 521
AfaI GTAC 1 cut(s) 223
AfiI CCNNNNNNNGG 3 cut(s) 123, 239, 596
AflII CTTAAG 1 cut(s) 749
AgeI ACCGGT 1 cut(s) 26
AgsI TTSAA 5 cut(s) 65, 392, 683, 721, 744
AjnI CCWGG 1 cut(s) 607
AluBI AGCT 9 cut(s) 17, 195, 356, 374, 413, 548, 563, 580, 652
AluI AGCT 9 cut(s) 17, 195, 356, 374, 413, 548, 563, 580, 652
Alw26I GTCTC 4 cut(s) 153, 163, 305, 443
Ama87I CYCGRG 3 cut(s) 118, 204, 644
AoxI GGCC 1 cut(s) 200
ApeKI GCWGC 4 cut(s) 181, 548, 560, 649
ApoI RAATTY 1 cut(s) 92
AsiGI ACCGGT 1 cut(s) 26
AsuHPI GGTGA 1 cut(s) 419
AvaI CYCGRG 3 cut(s) 118, 204, 644
BanII GRGCYC 1 cut(s) 420
BbsI GAAGAC 2 cut(s) 105, 728
BbvI GCAGC 4 cut(s) 193, 535, 572, 661
BccI CCATC 1 cut(s) 183
BceAI ACGGC 4 cut(s) 44, 149, 240, 330
BcgI CGANNNNNNTGC 6 cut(s) 126, 160, 170, 204, 421, 455
BciT130I CCWGG 1 cut(s) 609
BcoDI GTCTC 4 cut(s) 153, 163, 305, 443
BfmI CTRYAG 1 cut(s) 430
BfrI CTTAAG 1 cut(s) 749
BfuAI ACCTGC 1 cut(s) 670
BglII AGATCT 1 cut(s) 640
BisI GCNGC 8 cut(s) 6, 88, 135, 182, 228, 549, 561, 650
BlpI GCTNAGC 1 cut(s) 414
BlsI GCNGC 8 cut(s) 7, 89, 136, 183, 229, 550, 562, 651
Bme1390I CCNGG 1 cut(s) 609
BmeT110I CYCGRG 3 cut(s) 118, 204, 644
BmiI GGNNCC 1 cut(s) 231
BmrFI CCNGG 1 cut(s) 609
BmsI GCATC 5 cut(s) 226, 485, 560, 718, 753
BpiI GAAGAC 2 cut(s) 105, 728
BplI GAGNNNNNCTC 2 cut(s) 268, 300
BpmI CTGGAG 3 cut(s) 69, 216, 630
Bpu1102I GCTNAGC 1 cut(s) 414
BsaI GGTCTC 1 cut(s) 443
BsaJI CCNNGG 1 cut(s) 506
BsaWI WCCGGW 1 cut(s) 26
BsaXI ACNNNNNCTCC 4 cut(s) 214, 244, 603, 633
Bsc4I CCNNNNNNNGG 3 cut(s) 123, 239, 596
Bse118I RCCGGY 2 cut(s) 26, 239
BseBI CCWGG 1 cut(s) 609
BseDI CCNNGG 1 cut(s) 506
BseGI GGATG 1 cut(s) 265
BseLI CCNNNNNNNGG 3 cut(s) 123, 239, 596
BseMII CTCAG 2 cut(s) 299, 405
BseRI GAGGAG 3 cut(s) 133, 136, 311
BseXI GCAGC 4 cut(s) 193, 535, 572, 661
BsgI GTGCAG 1 cut(s) 648
Bsh1236I CGCG 1 cut(s) 90
BshFI GGCC 1 cut(s) 202
BshTI ACCGGT 1 cut(s) 26
BsiHKCI CYCGRG 3 cut(s) 118, 204, 644
BsiSI CCGG 2 cut(s) 27, 240
BslI CCNNNNNNNGG 3 cut(s) 123, 239, 596
BsmAI GTCTC 4 cut(s) 153, 163, 305, 443
BsmBI CGTCTC 2 cut(s) 153, 305
BsnI GGCC 1 cut(s) 202
Bso31I GGTCTC 1 cut(s) 443
BsoBI CYCGRG 3 cut(s) 118, 204, 644
Bsp1286I GDGCHC 1 cut(s) 420
Bsp143I GATC 2 cut(s) 460, 640
Bsp1720I GCTNAGC 1 cut(s) 414
BspACI CCGC 8 cut(s) 5, 85, 88, 134, 162, 227, 257, 766
BspANI GGCC 1 cut(s) 202
BspCNI CTCAG 2 cut(s) 298, 406
BspFNI CGCG 1 cut(s) 90
BspHI TCATGA 1 cut(s) 565
BspLI GGNNCC 1 cut(s) 231
BspMAI CTGCAG 1 cut(s) 434
BspMI ACCTGC 1 cut(s) 670
BspQI GCTCTTC 1 cut(s) 269
BspTI CTTAAG 1 cut(s) 749
BspTNI GGTCTC 1 cut(s) 443
BsrFI RCCGGY 2 cut(s) 26, 239
BssAI RCCGGY 2 cut(s) 26, 239
BssECI CCNNGG 1 cut(s) 506
BssMI GATC 2 cut(s) 460, 640
Bst2UI CCWGG 1 cut(s) 609
Bst4CI ACNGT 2 cut(s) 130, 250
Bst6I CTCTTC 3 cut(s) 156, 269, 288
BstAFI CTTAAG 1 cut(s) 749
BstAPI GCANNNNNTGC 1 cut(s) 763
BstC8I GCNNGC 2 cut(s) 241, 492
BstDEI CTNAG 2 cut(s) 285, 414
BstENI CCTNNNNNAGG 1 cut(s) 121
BstF5I GGATG 1 cut(s) 265
BstFNI CGCG 1 cut(s) 90
BstKTI GATC 2 cut(s) 463, 643
BstMAI GTCTC 4 cut(s) 153, 163, 305, 443
BstMBI GATC 2 cut(s) 460, 640
BstMWI GCNNNNNNNGC 5 cut(s) 14, 236, 557, 754, 763
BstNI CCWGG 1 cut(s) 609
BstSCI CCNGG 1 cut(s) 607
BstSFI CTRYAG 1 cut(s) 430
BstUI CGCG 1 cut(s) 90
BstV1I GCAGC 4 cut(s) 193, 535, 572, 661
BstV2I GAAGAC 2 cut(s) 105, 728
BstX2I RGATCY 1 cut(s) 640
BstYI RGATCY 1 cut(s) 640
BsuRI GGCC 1 cut(s) 202
BtgZI GCGATG 1 cut(s) 57
BtsCI GGATG 1 cut(s) 265
BtsI GCAGTG 1 cut(s) 439
BtsIMutI CAGTG 2 cut(s) 439, 486
BveI ACCTGC 1 cut(s) 670
Cac8I GCNNGC 2 cut(s) 241, 492
CciI TCATGA 1 cut(s) 565
Cfr10I RCCGGY 2 cut(s) 26, 239
CseI GACGC 2 cut(s) 566, 720
Csp6I GTAC 1 cut(s) 222
CspAI ACCGGT 1 cut(s) 26
CviAII CATG 2 cut(s) 531, 566
CviQI GTAC 1 cut(s) 222
DdeI CTNAG 2 cut(s) 285, 414
DpnI GATC 2 cut(s) 462, 642
DpnII GATC 2 cut(s) 460, 640
DrdI GACNNNNNNGTC 1 cut(s) 730
DseDI GACNNNNNNGTC 1 cut(s) 730
Eam1104I CTCTTC 3 cut(s) 156, 269, 288
EarI CTCTTC 3 cut(s) 156, 269, 288
EciI GGCGGA 2 cut(s) 74, 151
Eco24I GRGCYC 1 cut(s) 420
Eco31I GGTCTC 1 cut(s) 443
Eco57I CTGAAG 2 cut(s) 452, 521
Eco88I CYCGRG 3 cut(s) 118, 204, 644
EcoNI CCTNNNNNAGG 1 cut(s) 121
EcoRI GAATTC 1 cut(s) 92
EcoRII CCWGG 1 cut(s) 607
EcoT38I GRGCYC 1 cut(s) 420
Esp3I CGTCTC 2 cut(s) 153, 305
FaeI CATG 2 cut(s) 534, 569
FaiI YATR 9 cut(s) 169, 171, 332, 387, 450, 515, 532, 539, 567
FatI CATG 2 cut(s) 530, 565
FblI GTMKAC 1 cut(s) 399
Fnu4HI GCNGC 8 cut(s) 6, 88, 135, 182, 228, 549, 561, 650
FokI GGATG 1 cut(s) 272
FriOI GRGCYC 1 cut(s) 420
Fsp4HI GCNGC 8 cut(s) 6, 88, 135, 182, 228, 549, 561, 650
GluI GCNGC 8 cut(s) 6, 88, 135, 182, 228, 549, 561, 650
GsuI CTGGAG 3 cut(s) 69, 216, 630
HaeIII GGCC 1 cut(s) 202
HapII CCGG 2 cut(s) 27, 240
HgaI GACGC 2 cut(s) 566, 720
Hin1II CATG 2 cut(s) 534, 569
HincII GTYRAC 1 cut(s) 400
HindII GTYRAC 1 cut(s) 400
HindIII AAGCTT 1 cut(s) 354
HinfI GANTC 6 cut(s) 156, 263, 281, 319, 377, 423
HpaII CCGG 2 cut(s) 27, 240
HphI GGTGA 1 cut(s) 419
Hpy166II GTNNAC 1 cut(s) 400
Hpy188I TCNGA 5 cut(s) 36, 268, 288, 501, 556
Hpy188III TCNNGA 6 cut(s) 233, 458, 566, 644, 656, 819
Hpy8I GTNNAC 1 cut(s) 400
Hpy99I CGWCG 2 cut(s) 13, 250
HpyAV CCTTC 5 cut(s) 37, 283, 301, 476, 590
HpyCH4III ACNGT 2 cut(s) 130, 250
HpyCH4V TGCA 5 cut(s) 432, 476, 551, 665, 709
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 236, 557, 754, 763
HpyF3I CTNAG 2 cut(s) 285, 414
Hsp92II CATG 2 cut(s) 534, 569
KroI GCCGGC 1 cut(s) 239
KroNI GCCGGC 1 cut(s) 241
Kzo9I GATC 2 cut(s) 460, 640
LguI GCTCTTC 1 cut(s) 269
LmnI GCTCC 2 cut(s) 235, 754
Lsp1109I GCAGC 4 cut(s) 193, 535, 572, 661
LweI GCATC 5 cut(s) 226, 485, 560, 718, 753
MaeIII GTNAC 3 cut(s) 101, 629, 784
MalI GATC 2 cut(s) 462, 642
MboI GATC 2 cut(s) 460, 640
MflI RGATCY 1 cut(s) 640
MhlI GDGCHC 1 cut(s) 420
MluCI AATT 3 cut(s) 92, 345, 701
MlyI GAGTC 1 cut(s) 165
MroNI GCCGGC 1 cut(s) 239
MseI TTAA 3 cut(s) 525, 750, 800
MslI CAYNNNNRTG 1 cut(s) 570
MspA1I CMGCKG 1 cut(s) 257
MspCI CTTAAG 1 cut(s) 749
MspI CCGG 2 cut(s) 27, 240
MspR9I CCNGG 1 cut(s) 609
MvaI CCWGG 1 cut(s) 609
MvnI CGCG 1 cut(s) 90
MwoI GCNNNNNNNGC 5 cut(s) 14, 236, 557, 754, 763
NaeI GCCGGC 1 cut(s) 241
NdeII GATC 2 cut(s) 460, 640
NgoMIV GCCGGC 1 cut(s) 239
NlaIII CATG 2 cut(s) 534, 569
NlaIV GGNNCC 1 cut(s) 231
NmeAIII GCCGAG 1 cut(s) 531
PaeR7I CTCGAG 3 cut(s) 118, 204, 644
PagI TCATGA 1 cut(s) 565
PciSI GCTCTTC 1 cut(s) 269
PcsI WCGNNNNNNNCGW 1 cut(s) 116
PdiI GCCGGC 1 cut(s) 241
PfeI GAWTC 5 cut(s) 263, 281, 319, 377, 423
PfoI TCCNGGA 1 cut(s) 607
PinAI ACCGGT 1 cut(s) 26
PkrI GCNGC 8 cut(s) 7, 89, 136, 183, 229, 550, 562, 651
PleI GAGTC 1 cut(s) 164
PpsI GAGTC 1 cut(s) 164
Psp6I CCWGG 1 cut(s) 607
PspGI CCWGG 1 cut(s) 607
PspN4I GGNNCC 1 cut(s) 231
PspXI VCTCGAGB 2 cut(s) 118, 204
PstI CTGCAG 1 cut(s) 434
PsuI RGATCY 1 cut(s) 640
RsaI GTAC 1 cut(s) 223
RsaNI GTAC 1 cut(s) 222
RseI CAYNNNNRTG 1 cut(s) 570
SalI GTCGAC 1 cut(s) 398
SapI GCTCTTC 1 cut(s) 269
SaqAI TTAA 3 cut(s) 525, 750, 800
SatI GCNGC 8 cut(s) 6, 88, 135, 182, 228, 549, 561, 650
Sau3AI GATC 2 cut(s) 460, 640
SchI GAGTC 1 cut(s) 165
ScrFI CCNGG 1 cut(s) 609
SduI GDGCHC 1 cut(s) 420
SfaNI GCATC 5 cut(s) 226, 485, 560, 718, 753
SfcI CTRYAG 1 cut(s) 430
Sfr274I CTCGAG 3 cut(s) 118, 204, 644
SlaI CTCGAG 3 cut(s) 118, 204, 644
SmiMI CAYNNNNRTG 1 cut(s) 570
SmlI CTYRAG 4 cut(s) 118, 204, 644, 749
SmoI CTYRAG 4 cut(s) 118, 204, 644, 749
Sse9I AATT 3 cut(s) 92, 345, 701
SsiI CCGC 8 cut(s) 5, 85, 88, 134, 162, 227, 257, 766
StyD4I CCNGG 1 cut(s) 607
TaaI ACNGT 2 cut(s) 130, 250
TaqI TCGA 6 cut(s) 119, 154, 190, 205, 399, 645
TasI AATT 3 cut(s) 92, 345, 701
TauI GCSGC 4 cut(s) 8, 90, 137, 230
TfiI GAWTC 5 cut(s) 263, 281, 319, 377, 423
Tru1I TTAA 3 cut(s) 525, 750, 800
Tru9I TTAA 3 cut(s) 525, 750, 800
TscAI CASTG 2 cut(s) 439, 486
TseI GCWGC 4 cut(s) 181, 548, 560, 649
TspDTI ATGAA 2 cut(s) 276, 582
TspRI CASTG 2 cut(s) 439, 486
Vha464I CTTAAG 1 cut(s) 749
XagI CCTNNNNNAGG 1 cut(s) 121
XapI RAATTY 1 cut(s) 92
XhoI CTCGAG 3 cut(s) 118, 204, 644
XmiI GTMKAC 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.