Rroxscaffold_2G00156210

Aminoacyl tRNA synthase complex-interacting multifunctional protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014048
Physical Location & Seq
Reverse (-)
92691248 .. 92694814
3567 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00156210.1

Sequence Viewer

Length: 819 bp
ATGGCAGCGAACGTTTTGAAATGCGGCACGGGCATAGGCGCGCGTCTGATATTCCTATCTCCGTCTCCTAAATCTCCACGGCCCTTTGGGCATTCACTTGCTCATTTCCACCAAATCACTGCGAATAGTTATCGTAGTATTTGTAGCAAACCGAAAGTTTGTGATTCTGCTGCTGATGCATCATCACCATCGGCAAGAAGAAGAAGCGTGGTCTCTTTTTGCGCCGCCACAGATGTTGAGGAGGCACAGGTAGAGGCAGAGGCAGAGGCGATAAGCGATGGTGATGAGGAACAGAAAAAGATAAAGGATGCAGCCGACCTGCTTGACATAAGGGTTGGGCTAGTCCTTAGGGCATGGAGGCATGAGGAAGCTGATTCTCTTTACGTGGAAGAGGTTGACATCGGAGAGCCTGAACCCAGAATCATCTGCAGTGGGCTTGTCAATTACATTCCTATTGATCACATTCAGGAGAGAAAAGTAGTTGTCCTTGCTAATCTGAAGCCCAGGAACATGCGGGGTGTCAAGTCTTGTGGAATGCTTATGGCCGCTTCTGATGCTTCCCATAAGAATGTTGAGCTTCTTGTGCCTCCTGAGGGTTCACTTCCTGGCCAAAGGATATGGTTTGGCTCTGAAGATGATCATCAAAATCAGCCTCCTCCTGCCACACCTAACCAGATTCAAAAGAAAAAGATATGGGAGCTGGTGCAACCTCATCTGAAGACAGATGATAATTGTATGGCTATGCTTGGGATGCATATAATGCGGACATCTGCAGGTGTGATCATCAGCAGATCTCTAAAACACGCAAATATCTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_bind

Protein Analysis

272

Amino Acids

29.8

Weight (kDa)

6.55

Isoelectric Point (pI)

50.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_bind PF01588 110 - 204 9e-29 Putative tRNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014048)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59980
fragaria_vesca FvH4_1g00030 FvH4_1g00030 FvH4_1g00030
malus_domestica MD03G1140600.v1.1 MD11G1171300.v1.1
prunus_persica Prupe.6G126300_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0084251
rosa_laevigata RLG00000015603
rosa_multiflora Rmu_sc0003129.1_g000017
rosa_roxburghii Rroxscaffold_2G00156210
rosa_rugosa Rorug01G0452100
rosa_samantha Rh2AG000500 Rh2BG000400 Rh2CG000300 Rh2DG000500
rosa_wichuraiana Rw2G000030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 764
Acc36I ACCTGC 2 cut(s) 327, 764
AccII CGCG 2 cut(s) 41, 43
AciI CCGC 5 cut(s) 24, 225, 514, 546, 763
AclI AACGTT 1 cut(s) 12
AcoI YGGCCR 2 cut(s) 543, 607
AcuI CTGAAG 3 cut(s) 518, 651, 737
AfiI CCNNNNNNNGG 1 cut(s) 593
AgsI TTSAA 2 cut(s) 19, 680
AjnI CCWGG 2 cut(s) 503, 604
AluBI AGCT 3 cut(s) 371, 577, 700
AluI AGCT 3 cut(s) 371, 577, 700
Alw26I GTCTC 2 cut(s) 69, 217
AoxI GGCC 3 cut(s) 80, 543, 607
ApeKI GCWGC 3 cut(s) 5, 170, 311
AspLEI GCGC 3 cut(s) 41, 43, 224
AspS9I GGNCC 1 cut(s) 81
AsuHPI GGTGA 2 cut(s) 177, 293
AxyI CCTNAGG 2 cut(s) 347, 591
BalI TGGCCA 1 cut(s) 609
BbsI GAAGAC 1 cut(s) 725
BbvI GCAGC 3 cut(s) 17, 157, 323
BccI CCATC 2 cut(s) 196, 272
BceAI ACGGC 1 cut(s) 95
BciT130I CCWGG 2 cut(s) 505, 606
BclI TGATCA 3 cut(s) 457, 637, 780
BcoDI GTCTC 2 cut(s) 69, 217
BfaI CTAG 1 cut(s) 341
BfmI CTRYAG 2 cut(s) 427, 771
BfuAI ACCTGC 2 cut(s) 327, 764
BglI GCCNNNNNGGC 1 cut(s) 88
BglII AGATCT 1 cut(s) 791
BisI GCNGC 6 cut(s) 6, 25, 171, 225, 312, 546
BlsI GCNGC 6 cut(s) 7, 26, 172, 226, 313, 547
Bme1390I CCNGG 2 cut(s) 505, 606
BmgT120I GGNCC 1 cut(s) 81
BmrFI CCNGG 2 cut(s) 505, 606
BmsI GCATC 5 cut(s) 166, 188, 298, 544, 741
BpiI GAAGAC 1 cut(s) 725
BsaAI YACGTR 1 cut(s) 385
BsaBI GATNNNNATC 1 cut(s) 639
BsaI GGTCTC 1 cut(s) 217
BsaJI CCNNGG 2 cut(s) 77, 503
Bsc4I CCNNNNNNNGG 1 cut(s) 593
Bse21I CCTNAGG 2 cut(s) 347, 591
Bse8I GATNNNNATC 1 cut(s) 639
BseBI CCWGG 2 cut(s) 505, 606
BseDI CCNNGG 2 cut(s) 77, 503
BseGI GGATG 2 cut(s) 313, 756
BseJI GATNNNNATC 1 cut(s) 639
BseLI CCNNNNNNNGG 1 cut(s) 593
BseMII CTCAG 1 cut(s) 582
BsePI GCGCGC 1 cut(s) 39
BseRI GAGGAG 2 cut(s) 254, 645
BseXI GCAGC 3 cut(s) 17, 157, 323
Bsh1236I CGCG 2 cut(s) 41, 43
BshFI GGCC 3 cut(s) 82, 545, 609
BslI CCNNNNNNNGG 1 cut(s) 593
BsmAI GTCTC 2 cut(s) 69, 217
BsmBI CGTCTC 1 cut(s) 69
BsmI GAATGC 2 cut(s) 91, 540
BsnI GGCC 3 cut(s) 82, 545, 609
Bso31I GGTCTC 1 cut(s) 217
Bsp143I GATC 4 cut(s) 457, 637, 780, 791
BspACI CCGC 5 cut(s) 24, 225, 514, 546, 763
BspANI GGCC 3 cut(s) 82, 545, 609
BspCNI CTCAG 1 cut(s) 583
BspFNI CGCG 2 cut(s) 41, 43
BspMAI CTGCAG 2 cut(s) 431, 775
BspMI ACCTGC 2 cut(s) 327, 764
BspTNI GGTCTC 1 cut(s) 217
BssECI CCNNGG 2 cut(s) 77, 503
BssHII GCGCGC 1 cut(s) 39
BssMI GATC 4 cut(s) 457, 637, 780, 791
Bst2UI CCWGG 2 cut(s) 505, 606
Bst6I CTCTTC 1 cut(s) 384
BstAPI GCANNNNNTGC 1 cut(s) 760
BstBAI YACGTR 1 cut(s) 385
BstC8I GCNNGC 1 cut(s) 41
BstDEI CTNAG 2 cut(s) 347, 591
BstDSI CCRYGG 1 cut(s) 77
BstF5I GGATG 2 cut(s) 313, 756
BstFNI CGCG 2 cut(s) 41, 43
BstHHI GCGC 3 cut(s) 41, 43, 224
BstKTI GATC 4 cut(s) 460, 640, 783, 794
BstMAI GTCTC 2 cut(s) 69, 217
BstMBI GATC 4 cut(s) 457, 637, 780, 791
BstMWI GCNNNNNNNGC 7 cut(s) 30, 88, 176, 554, 583, 751, 760
BstNI CCWGG 2 cut(s) 505, 606
BstNSI RCATGY 1 cut(s) 514
BstSCI CCNGG 2 cut(s) 503, 604
BstSFI CTRYAG 2 cut(s) 427, 771
BstUI CGCG 2 cut(s) 41, 43
BstV1I GCAGC 3 cut(s) 17, 157, 323
BstV2I GAAGAC 1 cut(s) 725
BstX2I RGATCY 1 cut(s) 791
BstYI RGATCY 1 cut(s) 791
Bsu36I CCTNAGG 2 cut(s) 347, 591
BsuRI GGCC 3 cut(s) 82, 545, 609
BtgI CCRYGG 1 cut(s) 77
BtgZI GCGATG 1 cut(s) 291
BtsCI GGATG 2 cut(s) 313, 756
BtsI GCAGTG 2 cut(s) 117, 436
BtsIMutI CAGTG 2 cut(s) 117, 436
BveI ACCTGC 2 cut(s) 327, 764
Cac8I GCNNGC 1 cut(s) 41
CfoI GCGC 3 cut(s) 41, 43, 224
Cfr13I GGNCC 1 cut(s) 81
CseI GACGC 1 cut(s) 32
CspCI CAANNNNNGTGG 4 cut(s) 217, 252, 511, 546
CviAII CATG 3 cut(s) 354, 362, 511
DdeI CTNAG 2 cut(s) 347, 591
DpnI GATC 4 cut(s) 459, 639, 782, 793
DpnII GATC 4 cut(s) 457, 637, 780, 791
EaeI YGGCCR 2 cut(s) 543, 607
Eam1104I CTCTTC 1 cut(s) 384
EarI CTCTTC 1 cut(s) 384
Eco31I GGTCTC 1 cut(s) 217
Eco57I CTGAAG 3 cut(s) 518, 651, 737
Eco81I CCTNAGG 2 cut(s) 347, 591
EcoRII CCWGG 2 cut(s) 503, 604
EcoT22I ATGCAT 2 cut(s) 181, 756
Esp3I CGTCTC 1 cut(s) 69
FaeI CATG 3 cut(s) 357, 365, 514
FatI CATG 3 cut(s) 353, 361, 510
FauI CCCGC 1 cut(s) 507
FbaI TGATCA 3 cut(s) 457, 637, 780
Fnu4HI GCNGC 6 cut(s) 6, 25, 171, 225, 312, 546
FokI GGATG 2 cut(s) 320, 763
Fsp4HI GCNGC 6 cut(s) 6, 25, 171, 225, 312, 546
FspBI CTAG 1 cut(s) 341
GlaI GCGC 3 cut(s) 40, 42, 223
GluI GCNGC 6 cut(s) 6, 25, 171, 225, 312, 546
HaeIII GGCC 3 cut(s) 82, 545, 609
HgaI GACGC 1 cut(s) 32
HhaI GCGC 3 cut(s) 41, 43, 224
Hin1II CATG 3 cut(s) 357, 365, 514
Hin6I GCGC 3 cut(s) 39, 41, 222
HinP1I GCGC 3 cut(s) 39, 41, 222
HincII GTYRAC 1 cut(s) 397
HindII GTYRAC 1 cut(s) 397
HinfI GANTC 4 cut(s) 164, 374, 420, 676
HphI GGTGA 2 cut(s) 177, 293
Hpy166II GTNNAC 2 cut(s) 397, 599
Hpy188I TCNGA 6 cut(s) 48, 404, 498, 553, 631, 717
Hpy188III TCNNGA 2 cut(s) 467, 590
Hpy8I GTNNAC 2 cut(s) 397, 599
HpyCH4IV ACGT 2 cut(s) 12, 384
HpyCH4V TGCA 6 cut(s) 179, 311, 429, 706, 754, 773
HpyF10VI GCNNNNNNNGC 7 cut(s) 30, 88, 176, 554, 583, 751, 760
HpyF3I CTNAG 2 cut(s) 347, 591
HpySE526I ACGT 2 cut(s) 12, 384
Hsp92II CATG 3 cut(s) 357, 365, 514
HspAI GCGC 3 cut(s) 39, 41, 222
Ksp22I TGATCA 3 cut(s) 457, 637, 780
Kzo9I GATC 4 cut(s) 457, 637, 780, 791
LmnI GCTCC 1 cut(s) 697
Lsp1109I GCAGC 3 cut(s) 17, 157, 323
LweI GCATC 5 cut(s) 166, 188, 298, 544, 741
MaeI CTAG 1 cut(s) 341
MaeII ACGT 2 cut(s) 12, 384
MalI GATC 4 cut(s) 459, 639, 782, 793
MboI GATC 4 cut(s) 457, 637, 780, 791
MboII GAAGA 5 cut(s) 210, 213, 401, 644, 730
MflI RGATCY 1 cut(s) 791
MlsI TGGCCA 1 cut(s) 609
MluCI AATT 2 cut(s) 442, 730
MluNI TGGCCA 1 cut(s) 609
Mox20I TGGCCA 1 cut(s) 609
Mph1103I ATGCAT 2 cut(s) 181, 756
MscI TGGCCA 1 cut(s) 609
MslI CAYNNNNRTG 1 cut(s) 567
Msp20I TGGCCA 1 cut(s) 609
MspR9I CCNGG 2 cut(s) 505, 606
Mva1269I GAATGC 2 cut(s) 91, 540
MvaI CCWGG 2 cut(s) 505, 606
MvnI CGCG 2 cut(s) 41, 43
MwoI GCNNNNNNNGC 7 cut(s) 30, 88, 176, 554, 583, 751, 760
NdeII GATC 4 cut(s) 457, 637, 780, 791
NlaIII CATG 3 cut(s) 357, 365, 514
NsiI ATGCAT 2 cut(s) 181, 756
NspI RCATGY 1 cut(s) 514
PaqCI CACCTGC 1 cut(s) 764
PauI GCGCGC 1 cut(s) 39
PctI GAATGC 2 cut(s) 91, 540
PfeI GAWTC 4 cut(s) 164, 374, 420, 676
PkrI GCNGC 6 cut(s) 7, 26, 172, 226, 313, 547
Ppu21I YACGTR 1 cut(s) 385
Psp1406I AACGTT 1 cut(s) 12
Psp6I CCWGG 2 cut(s) 503, 604
PspGI CCWGG 2 cut(s) 503, 604
PspPI GGNCC 1 cut(s) 81
PstI CTGCAG 2 cut(s) 431, 775
PsuI RGATCY 1 cut(s) 791
PteI GCGCGC 1 cut(s) 39
RseI CAYNNNNRTG 1 cut(s) 567
SatI GCNGC 6 cut(s) 6, 25, 171, 225, 312, 546
Sau3AI GATC 4 cut(s) 457, 637, 780, 791
Sau96I GGNCC 1 cut(s) 81
ScrFI CCNGG 2 cut(s) 505, 606
SfaNI GCATC 5 cut(s) 166, 188, 298, 544, 741
SfcI CTRYAG 2 cut(s) 427, 771
SmiMI CAYNNNNRTG 1 cut(s) 567
Sse9I AATT 2 cut(s) 442, 730
SsiI CCGC 5 cut(s) 24, 225, 514, 546, 763
SspMI CTAG 1 cut(s) 341
StyD4I CCNGG 2 cut(s) 503, 604
TaiI ACGT 2 cut(s) 15, 387
TasI AATT 2 cut(s) 442, 730
TauI GCSGC 3 cut(s) 27, 227, 548
TfiI GAWTC 4 cut(s) 164, 374, 420, 676
TscAI CASTG 2 cut(s) 124, 436
TseI GCWGC 3 cut(s) 5, 170, 311
TspGWI ACGGA 1 cut(s) 51
TspRI CASTG 2 cut(s) 124, 436
XceI RCATGY 1 cut(s) 514
XspI CTAG 1 cut(s) 341
Zsp2I ATGCAT 2 cut(s) 181, 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.