Rh2DG000500

Aminoacyl tRNA synthase complex-interacting multifunctional protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0014048
Physical Location & Seq
Forward (+)
62014 .. 64001
1988 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG000500.1

Sequence Viewer

Length: 687 bp
ATGGCAGCGAACGTTTTGAAATGCGGCACGGGCATAGGCGCGCGTCTGATATTCCTATCTCCGTCTCCTAAATCTCCACGGCCCTTTGAGCATTCACTTGCTCATTTCCACCAAATCACTGCGAATAGTTATCGCAGTATTTGTAGCAAACCGAAAGTTTGTGATTCTGCTGCTGATGTGTCATCACCATCGGCAAGAAGAAGAAGCGTGGTCTCCTCTTTTTGCGCCGCCACAGATGTTGAGGAGGCACAGGTAGAGGCAGAGGCGATAAGCGATGGTGATGAGGAACAGAAAAAGATAAAGGATGCAGCCGACCTGCTTGACATAAGGGTTGGGCTAGTCCTTAGGGCATGGAGGCATGAGGAAGCTGATTCTCTTTACGTGGAAGAGGTTGACATCGGAGAGCCTGAACCCAGAATCATCTGCAGTGGGCTTGTCAATTACATTCCTATTGATCACATTCAGGAGAGAAAAGTAGTTGTCCTTGCTAATCTGAAGCCCAGGAACATGCGGGGTGTCAAGTCTTGTGGAATGCTTATGGCCGCTTCTGATGCTTCCCATGAGAGTGTTGAGCTTCTTGTGCCTCCTGAGGGTTCACTTCCTGGCCAAAGGATATGGTTTGGCTCTGAAGATGATCATCAAAATCAGCCTCCTCCTGCCACACCTAACCAGGGTCGAAGCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_bind

Protein Analysis

228

Amino Acids

24.87

Weight (kDa)

5.63

Isoelectric Point (pI)

50.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_bind PF01588 109 - 203 9e-29 Putative tRNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014048)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59980
fragaria_vesca FvH4_1g00030 FvH4_1g00030 FvH4_1g00030
malus_domestica MD03G1140600.v1.1 MD11G1171300.v1.1
prunus_persica Prupe.6G126300_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0084251
rosa_laevigata RLG00000015603
rosa_multiflora Rmu_sc0003129.1_g000017
rosa_roxburghii Rroxscaffold_2G00156210
rosa_rugosa Rorug01G0452100
rosa_samantha Rh2AG000500 Rh2BG000400 Rh2CG000300 Rh2DG000500
rosa_wichuraiana Rw2G000030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 324
AccII CGCG 2 cut(s) 41, 43
AciI CCGC 4 cut(s) 24, 228, 511, 543
AclI AACGTT 1 cut(s) 12
AcoI YGGCCR 2 cut(s) 540, 604
AcuI CTGAAG 2 cut(s) 515, 648
AfiI CCNNNNNNNGG 2 cut(s) 590, 671
AgsI TTSAA 1 cut(s) 19
AjnI CCWGG 3 cut(s) 500, 601, 669
AluBI AGCT 2 cut(s) 368, 574
AluI AGCT 2 cut(s) 368, 574
Alw26I GTCTC 2 cut(s) 69, 217
AoxI GGCC 3 cut(s) 80, 540, 604
ApeKI GCWGC 3 cut(s) 5, 170, 308
AspLEI GCGC 3 cut(s) 41, 43, 227
AspS9I GGNCC 1 cut(s) 81
AsuHPI GGTGA 2 cut(s) 177, 290
AxyI CCTNAGG 2 cut(s) 344, 588
BalI TGGCCA 1 cut(s) 606
BbvI GCAGC 3 cut(s) 17, 157, 320
BccI CCATC 2 cut(s) 196, 269
BceAI ACGGC 1 cut(s) 95
BciT130I CCWGG 3 cut(s) 502, 603, 671
BclI TGATCA 2 cut(s) 454, 634
BcoDI GTCTC 2 cut(s) 69, 217
BfaI CTAG 1 cut(s) 338
BfmI CTRYAG 1 cut(s) 424
BfuAI ACCTGC 1 cut(s) 324
BisI GCNGC 6 cut(s) 6, 25, 171, 228, 309, 543
BlsI GCNGC 6 cut(s) 7, 26, 172, 229, 310, 544
Bme1390I CCNGG 3 cut(s) 502, 603, 671
BmgT120I GGNCC 1 cut(s) 81
BmrFI CCNGG 3 cut(s) 502, 603, 671
BmsI GCATC 2 cut(s) 295, 541
BsaAI YACGTR 1 cut(s) 382
BsaBI GATNNNNATC 1 cut(s) 636
BsaI GGTCTC 1 cut(s) 217
BsaJI CCNNGG 3 cut(s) 77, 500, 670
Bsc4I CCNNNNNNNGG 2 cut(s) 590, 671
Bse21I CCTNAGG 2 cut(s) 344, 588
Bse8I GATNNNNATC 1 cut(s) 636
BseBI CCWGG 3 cut(s) 502, 603, 671
BseDI CCNNGG 3 cut(s) 77, 500, 670
BseGI GGATG 1 cut(s) 310
BseJI GATNNNNATC 1 cut(s) 636
BseLI CCNNNNNNNGG 2 cut(s) 590, 671
BseMII CTCAG 1 cut(s) 579
BsePI GCGCGC 1 cut(s) 39
BseRI GAGGAG 3 cut(s) 205, 257, 642
BseXI GCAGC 3 cut(s) 17, 157, 320
Bsh1236I CGCG 2 cut(s) 41, 43
BshFI GGCC 3 cut(s) 82, 542, 606
BslI CCNNNNNNNGG 2 cut(s) 590, 671
BsmAI GTCTC 2 cut(s) 69, 217
BsmBI CGTCTC 1 cut(s) 69
BsmI GAATGC 2 cut(s) 91, 537
BsnI GGCC 3 cut(s) 82, 542, 606
Bso31I GGTCTC 1 cut(s) 217
Bsp143I GATC 2 cut(s) 454, 634
BspACI CCGC 4 cut(s) 24, 228, 511, 543
BspANI GGCC 3 cut(s) 82, 542, 606
BspCNI CTCAG 1 cut(s) 580
BspFNI CGCG 2 cut(s) 41, 43
BspMAI CTGCAG 1 cut(s) 428
BspMI ACCTGC 1 cut(s) 324
BspTNI GGTCTC 1 cut(s) 217
BssECI CCNNGG 3 cut(s) 77, 500, 670
BssHII GCGCGC 1 cut(s) 39
BssMI GATC 2 cut(s) 454, 634
Bst2UI CCWGG 3 cut(s) 502, 603, 671
Bst6I CTCTTC 1 cut(s) 381
BstBAI YACGTR 1 cut(s) 382
BstC8I GCNNGC 1 cut(s) 41
BstDEI CTNAG 2 cut(s) 344, 588
BstDSI CCRYGG 1 cut(s) 77
BstF5I GGATG 1 cut(s) 310
BstFNI CGCG 2 cut(s) 41, 43
BstHHI GCGC 3 cut(s) 41, 43, 227
BstKTI GATC 2 cut(s) 457, 637
BstMAI GTCTC 2 cut(s) 69, 217
BstMBI GATC 2 cut(s) 454, 634
BstMWI GCNNNNNNNGC 4 cut(s) 30, 88, 551, 580
BstNI CCWGG 3 cut(s) 502, 603, 671
BstNSI RCATGY 1 cut(s) 511
BstSCI CCNGG 3 cut(s) 500, 601, 669
BstSFI CTRYAG 1 cut(s) 424
BstUI CGCG 2 cut(s) 41, 43
BstV1I GCAGC 3 cut(s) 17, 157, 320
Bsu36I CCTNAGG 2 cut(s) 344, 588
BsuRI GGCC 3 cut(s) 82, 542, 606
BtgI CCRYGG 1 cut(s) 77
BtgZI GCGATG 1 cut(s) 288
BtsCI GGATG 1 cut(s) 310
BtsI GCAGTG 2 cut(s) 117, 433
BtsIMutI CAGTG 2 cut(s) 117, 433
BveI ACCTGC 1 cut(s) 324
Cac8I GCNNGC 1 cut(s) 41
CfoI GCGC 3 cut(s) 41, 43, 227
Cfr13I GGNCC 1 cut(s) 81
CseI GACGC 1 cut(s) 32
CspCI CAANNNNNGTGG 4 cut(s) 220, 255, 508, 543
CviAII CATG 4 cut(s) 351, 359, 508, 560
DdeI CTNAG 2 cut(s) 344, 588
DpnI GATC 2 cut(s) 456, 636
DpnII GATC 2 cut(s) 454, 634
EaeI YGGCCR 2 cut(s) 540, 604
Eam1104I CTCTTC 1 cut(s) 381
EarI CTCTTC 1 cut(s) 381
Eco31I GGTCTC 1 cut(s) 217
Eco57I CTGAAG 2 cut(s) 515, 648
Eco81I CCTNAGG 2 cut(s) 344, 588
EcoRII CCWGG 3 cut(s) 500, 601, 669
Esp3I CGTCTC 1 cut(s) 69
FaeI CATG 4 cut(s) 354, 362, 511, 563
FaiI YATR 8 cut(s) 35, 326, 352, 360, 509, 539, 561, 616
FatI CATG 4 cut(s) 350, 358, 507, 559
FauI CCCGC 1 cut(s) 504
FbaI TGATCA 2 cut(s) 454, 634
Fnu4HI GCNGC 6 cut(s) 6, 25, 171, 228, 309, 543
FokI GGATG 1 cut(s) 317
Fsp4HI GCNGC 6 cut(s) 6, 25, 171, 228, 309, 543
FspBI CTAG 1 cut(s) 338
GlaI GCGC 3 cut(s) 40, 42, 226
GluI GCNGC 6 cut(s) 6, 25, 171, 228, 309, 543
HaeIII GGCC 3 cut(s) 82, 542, 606
HgaI GACGC 1 cut(s) 32
HhaI GCGC 3 cut(s) 41, 43, 227
Hin1II CATG 4 cut(s) 354, 362, 511, 563
Hin6I GCGC 3 cut(s) 39, 41, 225
HinP1I GCGC 3 cut(s) 39, 41, 225
HincII GTYRAC 1 cut(s) 394
HindII GTYRAC 1 cut(s) 394
HinfI GANTC 3 cut(s) 164, 371, 417
HphI GGTGA 2 cut(s) 177, 290
Hpy166II GTNNAC 2 cut(s) 394, 596
Hpy188I TCNGA 5 cut(s) 48, 401, 495, 550, 628
Hpy188III TCNNGA 2 cut(s) 464, 587
Hpy8I GTNNAC 2 cut(s) 394, 596
HpyCH4IV ACGT 2 cut(s) 12, 381
HpyCH4V TGCA 2 cut(s) 308, 426
HpyF10VI GCNNNNNNNGC 4 cut(s) 30, 88, 551, 580
HpyF3I CTNAG 2 cut(s) 344, 588
HpySE526I ACGT 2 cut(s) 12, 381
Hsp92II CATG 4 cut(s) 354, 362, 511, 563
HspAI GCGC 3 cut(s) 39, 41, 225
Ksp22I TGATCA 2 cut(s) 454, 634
Kzo9I GATC 2 cut(s) 454, 634
Lsp1109I GCAGC 3 cut(s) 17, 157, 320
LweI GCATC 2 cut(s) 295, 541
MaeI CTAG 1 cut(s) 338
MaeII ACGT 2 cut(s) 12, 381
MalI GATC 2 cut(s) 456, 636
MboI GATC 2 cut(s) 454, 634
MboII GAAGA 4 cut(s) 210, 213, 398, 641
MlsI TGGCCA 1 cut(s) 606
MluCI AATT 1 cut(s) 439
MluNI TGGCCA 1 cut(s) 606
Mox20I TGGCCA 1 cut(s) 606
MscI TGGCCA 1 cut(s) 606
MslI CAYNNNNRTG 1 cut(s) 564
Msp20I TGGCCA 1 cut(s) 606
MspR9I CCNGG 3 cut(s) 502, 603, 671
Mva1269I GAATGC 2 cut(s) 91, 537
MvaI CCWGG 3 cut(s) 502, 603, 671
MvnI CGCG 2 cut(s) 41, 43
MwoI GCNNNNNNNGC 4 cut(s) 30, 88, 551, 580
NdeII GATC 2 cut(s) 454, 634
NlaIII CATG 4 cut(s) 354, 362, 511, 563
NspI RCATGY 1 cut(s) 511
PauI GCGCGC 1 cut(s) 39
PctI GAATGC 2 cut(s) 91, 537
PfeI GAWTC 3 cut(s) 164, 371, 417
PkrI GCNGC 6 cut(s) 7, 26, 172, 229, 310, 544
Ppu21I YACGTR 1 cut(s) 382
Psp1406I AACGTT 1 cut(s) 12
Psp6I CCWGG 3 cut(s) 500, 601, 669
PspGI CCWGG 3 cut(s) 500, 601, 669
PspPI GGNCC 1 cut(s) 81
PstI CTGCAG 1 cut(s) 428
PteI GCGCGC 1 cut(s) 39
RseI CAYNNNNRTG 1 cut(s) 564
SatI GCNGC 6 cut(s) 6, 25, 171, 228, 309, 543
Sau3AI GATC 2 cut(s) 454, 634
Sau96I GGNCC 1 cut(s) 81
ScrFI CCNGG 3 cut(s) 502, 603, 671
SetI ASST 8 cut(s) 15, 255, 318, 370, 384, 393, 576, 667
SfaNI GCATC 2 cut(s) 295, 541
SfcI CTRYAG 1 cut(s) 424
SmiMI CAYNNNNRTG 1 cut(s) 564
Sse9I AATT 1 cut(s) 439
SsiI CCGC 4 cut(s) 24, 228, 511, 543
SspMI CTAG 1 cut(s) 338
StyD4I CCNGG 3 cut(s) 500, 601, 669
TaiI ACGT 2 cut(s) 15, 384
TaqI TCGA 1 cut(s) 676
TasI AATT 1 cut(s) 439
TauI GCSGC 3 cut(s) 27, 230, 545
TfiI GAWTC 3 cut(s) 164, 371, 417
TscAI CASTG 2 cut(s) 124, 433
TseI GCWGC 3 cut(s) 5, 170, 308
TspGWI ACGGA 1 cut(s) 51
TspRI CASTG 2 cut(s) 124, 433
XceI RCATGY 1 cut(s) 511
XspI CTAG 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.