MD03G1140600.v1.1

Aminoacyl tRNA synthase complex-interacting multifunctional protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014048
Physical Location & Seq
Forward (+)
14784829 .. 14789128
4300 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1140600.v1.1.491

Sequence Viewer

Length: 855 bp
ATGGCTGCAACGACCCTTTTGAAAGGAAGCTGCACCAGCATCAGTGTCCATCGTCTCTTCACCTTCGCCCACGCGCACCGCCCTCTCAATCTCCACCAAATCAATTCCGCCGTCAATCAAAATTACTATCACATCCGCATTCGCCGCAGAAAACCCACAATATCACCACCACCAAGACTCGCTGCCTTGTGGCACTCATCTTCTTCCTTCTGCACCGCCGCCTCGGCCGACGACGCTTCTGCAACTGCTGCTACTGCTTCTTCTGAGGCAGTGAGGGATGCTGCTGTGGTCGACGAGGCGGCGCCGGCAAGCGAGGTTGTCGATGAACAGAAAAAGATAAAGGATGCAGCCGACGTGCTCGACATAAGGGTTGGCCGAGTTGTTAAGGCGTGGAGGCATGAGGAGGCTGATTCTCTTTATGTGGAGGAAGTTGACGTCGGCGAGCCCGAACCCAGAACCATCTGCAGTGGCCTTGTCAAATATGTGCCTCTTGATCACCTTCAGGACAGAAATGTAGTTGTGCTTGCTAATCTGAAGCCGAGGAACATGCGCGGTGTGAAGTCTAATGGAATGCTTATGGCAGCTTCGGATGCTTCCCATGAGAATGTTGAGCTTCTTGTGCCACCTGAGGGTTCACTCCCTGGCCAAAGAGTATGGTTTGGCTCTGAAGAGGATCATCAAAATCAGCCTCCTCCTGCCTCGCCTAACCAGATTCAAAAGAAAAGGATATGGGAATTGGTGCAACCTCATTTGAAGACCGACGATTCTTGTATTGCTTTACTTGGGGAGCATGTAATGCGGACATCTGCAGGTCTGGTAACCTCAACATCTCTGAAAAACGCAAATATCTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_bind

Protein Analysis

285

Amino Acids

31.03

Weight (kDa)

6.64

Isoelectric Point (pI)

45.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_bind PF01588 122 - 216 2.3e-31 Putative tRNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014048)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59980
fragaria_vesca FvH4_1g00030 FvH4_1g00030 FvH4_1g00030
malus_domestica MD03G1140600.v1.1 MD11G1171300.v1.1
prunus_persica Prupe.6G126300_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0084251
rosa_laevigata RLG00000015603
rosa_multiflora Rmu_sc0003129.1_g000017
rosa_roxburghii Rroxscaffold_2G00156210
rosa_rugosa Rorug01G0452100
rosa_samantha Rh2AG000500 Rh2BG000400 Rh2CG000300 Rh2DG000500
rosa_wichuraiana Rw2G000030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 438
Acc36I ACCTGC 1 cut(s) 800
AccB1I GGYRCC 1 cut(s) 301
AccI GTMKAC 1 cut(s) 291
AccII CGCG 2 cut(s) 74, 552
AciI CCGC 9 cut(s) 79, 108, 136, 145, 216, 219, 299, 552, 799
AclWI GGATC 1 cut(s) 681
AcoI YGGCCR 3 cut(s) 225, 373, 643
AcuI CTGAAG 3 cut(s) 485, 554, 687
AcyI GRCGYC 2 cut(s) 302, 435
AfiI CCNNNNNNNGG 1 cut(s) 629
AgsI TTSAA 3 cut(s) 22, 716, 754
AjiI CACGTC 1 cut(s) 355
AjnI CCWGG 1 cut(s) 640
AluBI AGCT 3 cut(s) 30, 584, 613
AluI AGCT 3 cut(s) 30, 584, 613
Alw21I GWGCWC 1 cut(s) 360
Alw26I GTCTC 1 cut(s) 59
AlwI GGATC 1 cut(s) 681
AoxI GGCC 4 cut(s) 225, 373, 469, 643
ApeKI GCWGC 7 cut(s) 5, 30, 182, 248, 281, 347, 581
AspLEI GCGC 3 cut(s) 76, 304, 552
AsuHPI GGTGA 3 cut(s) 52, 156, 488
AxyI CCTNAGG 1 cut(s) 627
BalI TGGCCA 1 cut(s) 645
BanI GGYRCC 1 cut(s) 301
BanII GRGCYC 1 cut(s) 447
BbsI GAAGAC 1 cut(s) 761
Bbv12I GWGCWC 1 cut(s) 360
BbvI GCAGC 6 cut(s) 17, 169, 235, 268, 359, 593
BccI CCATC 2 cut(s) 57, 467
BceAI ACGGC 1 cut(s) 95
BcgI CGANNNNNNTGC 5 cut(s) 221, 255, 529, 563, 831
BciT130I CCWGG 1 cut(s) 642
BclI TGATCA 1 cut(s) 493
BcoDI GTCTC 1 cut(s) 59
BfmI CTRYAG 2 cut(s) 463, 807
BfoI RGCGCY 1 cut(s) 305
BfuAI ACCTGC 1 cut(s) 800
BglI GCCNNNNNGGC 1 cut(s) 224
Bme1390I CCNGG 1 cut(s) 642
BmgBI CACGTC 1 cut(s) 355
BmiI GGNNCC 1 cut(s) 303
BmrFI CCNGG 1 cut(s) 642
BmsI GCATC 4 cut(s) 48, 268, 334, 580
BpiI GAAGAC 1 cut(s) 761
BsaHI GRCGYC 2 cut(s) 302, 435
BsaJI CCNNGG 3 cut(s) 222, 539, 640
Bsc4I CCNNNNNNNGG 1 cut(s) 629
Bse118I RCCGGY 1 cut(s) 304
Bse21I CCTNAGG 1 cut(s) 627
BseBI CCWGG 1 cut(s) 642
BseDI CCNNGG 3 cut(s) 222, 539, 640
BseGI GGATG 4 cut(s) 132, 283, 349, 595
BseLI CCNNNNNNNGG 1 cut(s) 629
BseMII CTCAG 2 cut(s) 255, 618
BseRI GAGGAG 2 cut(s) 416, 681
BseX3I CGGCCG 1 cut(s) 225
BseXI GCAGC 6 cut(s) 17, 169, 235, 268, 359, 593
BsgI GTGCAG 2 cut(s) 16, 196
Bsh1236I CGCG 2 cut(s) 74, 552
Bsh1285I CGRYCG 1 cut(s) 228
BshFI GGCC 4 cut(s) 227, 375, 471, 645
BshNI GGYRCC 1 cut(s) 301
BsiEI CGRYCG 1 cut(s) 228
BsiHKAI GWGCWC 1 cut(s) 360
BsiSI CCGG 1 cut(s) 305
BslI CCNNNNNNNGG 1 cut(s) 629
BsmAI GTCTC 1 cut(s) 59
BsmBI CGTCTC 1 cut(s) 59
BsmI GAATGC 2 cut(s) 138, 576
BsnI GGCC 4 cut(s) 227, 375, 471, 645
Bsp1286I GDGCHC 2 cut(s) 360, 447
Bsp143I GATC 2 cut(s) 493, 673
BspACI CCGC 9 cut(s) 79, 108, 136, 145, 216, 219, 299, 552, 799
BspANI GGCC 4 cut(s) 227, 375, 471, 645
BspCNI CTCAG 2 cut(s) 256, 619
BspFNI CGCG 2 cut(s) 74, 552
BspLI GGNNCC 1 cut(s) 303
BspMAI CTGCAG 2 cut(s) 467, 811
BspMI ACCTGC 1 cut(s) 800
BspPI GGATC 1 cut(s) 681
BspT107I GGYRCC 1 cut(s) 301
BsrFI RCCGGY 1 cut(s) 304
BssAI RCCGGY 1 cut(s) 304
BssECI CCNNGG 3 cut(s) 222, 539, 640
BssMI GATC 2 cut(s) 493, 673
BssNI GRCGYC 2 cut(s) 302, 435
Bst2UI CCWGG 1 cut(s) 642
Bst6I CTCTTC 2 cut(s) 62, 663
BstACI GRCGYC 2 cut(s) 302, 435
BstAPI GCANNNNNTGC 2 cut(s) 248, 796
BstC8I GCNNGC 4 cut(s) 306, 310, 443, 525
BstDEI CTNAG 2 cut(s) 264, 627
BstEII GGTNACC 1 cut(s) 817
BstF5I GGATG 4 cut(s) 132, 283, 349, 595
BstFNI CGCG 2 cut(s) 74, 552
BstH2I RGCGCY 1 cut(s) 305
BstHHI GCGC 3 cut(s) 76, 304, 552
BstKTI GATC 2 cut(s) 496, 676
BstMAI GTCTC 1 cut(s) 59
BstMBI GATC 2 cut(s) 493, 673
BstMCI CGRYCG 1 cut(s) 228
BstNI CCWGG 1 cut(s) 642
BstNSI RCATGY 2 cut(s) 550, 794
BstPI GGTNACC 1 cut(s) 817
BstSCI CCNGG 1 cut(s) 640
BstSFI CTRYAG 2 cut(s) 463, 807
BstUI CGCG 2 cut(s) 74, 552
BstV1I GCAGC 6 cut(s) 17, 169, 235, 268, 359, 593
BstV2I GAAGAC 1 cut(s) 761
BstZI CGGCCG 1 cut(s) 225
Bsu36I CCTNAGG 1 cut(s) 627
BsuRI GGCC 4 cut(s) 227, 375, 471, 645
BtrI CACGTC 1 cut(s) 355
BtsCI GGATG 4 cut(s) 132, 283, 349, 595
BtsI GCAGTG 2 cut(s) 276, 472
BtsIMutI CAGTG 3 cut(s) 49, 276, 472
BveI ACCTGC 1 cut(s) 800
Cac8I GCNNGC 4 cut(s) 306, 310, 443, 525
CfoI GCGC 3 cut(s) 76, 304, 552
Cfr10I RCCGGY 1 cut(s) 304
CseI GACGC 1 cut(s) 242
CviAII CATG 4 cut(s) 398, 547, 599, 791
DdeI CTNAG 2 cut(s) 264, 627
DinI GGCGCC 1 cut(s) 303
DpnI GATC 2 cut(s) 495, 675
DpnII GATC 2 cut(s) 493, 673
EaeI YGGCCR 3 cut(s) 225, 373, 643
EagI CGGCCG 1 cut(s) 225
Eam1104I CTCTTC 2 cut(s) 62, 663
EarI CTCTTC 2 cut(s) 62, 663
EciI GGCGGA 1 cut(s) 97
EclXI CGGCCG 1 cut(s) 225
Eco24I GRGCYC 1 cut(s) 447
Eco52I CGGCCG 1 cut(s) 225
Eco57I CTGAAG 3 cut(s) 485, 554, 687
Eco81I CCTNAGG 1 cut(s) 627
Eco91I GGTNACC 1 cut(s) 817
EcoO65I GGTNACC 1 cut(s) 817
EcoRII CCWGG 1 cut(s) 640
EcoT38I GRGCYC 1 cut(s) 447
EgeI GGCGCC 1 cut(s) 303
EheI GGCGCC 1 cut(s) 303
Esp3I CGTCTC 1 cut(s) 59
FaeI CATG 4 cut(s) 401, 550, 602, 794
FatI CATG 4 cut(s) 397, 546, 598, 790
FbaI TGATCA 1 cut(s) 493
FblI GTMKAC 1 cut(s) 291
FokI GGATG 4 cut(s) 119, 290, 356, 602
FriOI GRGCYC 1 cut(s) 447
GlaI GCGC 3 cut(s) 75, 303, 551
HaeII RGCGCY 1 cut(s) 305
HaeIII GGCC 4 cut(s) 227, 375, 471, 645
HapII CCGG 1 cut(s) 305
HgaI GACGC 1 cut(s) 242
HhaI GCGC 3 cut(s) 76, 304, 552
Hin1I GRCGYC 2 cut(s) 302, 435
Hin1II CATG 4 cut(s) 401, 550, 602, 794
Hin6I GCGC 3 cut(s) 74, 302, 550
HinP1I GCGC 3 cut(s) 74, 302, 550
HincII GTYRAC 2 cut(s) 292, 433
HindII GTYRAC 2 cut(s) 292, 433
HinfI GANTC 4 cut(s) 177, 410, 712, 764
HpaII CCGG 1 cut(s) 305
HphI GGTGA 3 cut(s) 52, 156, 488
Hpy166II GTNNAC 3 cut(s) 292, 433, 635
Hpy188I TCNGA 5 cut(s) 265, 534, 589, 667, 834
Hpy188III TCNNGA 2 cut(s) 491, 503
Hpy8I GTNNAC 3 cut(s) 292, 433, 635
Hpy99I CGWCG 6 cut(s) 233, 236, 296, 356, 440, 764
HpyAV CCTTC 3 cut(s) 73, 217, 509
HpyCH4IV ACGT 2 cut(s) 354, 435
HpyCH4V TGCA 8 cut(s) 8, 33, 213, 242, 347, 465, 742, 809
HpyF3I CTNAG 2 cut(s) 264, 627
HpySE526I ACGT 2 cut(s) 354, 435
Hsp92I GRCGYC 2 cut(s) 302, 435
Hsp92II CATG 4 cut(s) 401, 550, 602, 794
HspAI GCGC 3 cut(s) 74, 302, 550
KasI GGCGCC 1 cut(s) 301
KroI GCCGGC 1 cut(s) 304
KroNI GCCGGC 1 cut(s) 306
Ksp22I TGATCA 1 cut(s) 493
Kzo9I GATC 2 cut(s) 493, 673
LmnI GCTCC 1 cut(s) 787
Lsp1109I GCAGC 6 cut(s) 17, 169, 235, 268, 359, 593
LweI GCATC 4 cut(s) 48, 268, 334, 580
MaeII ACGT 2 cut(s) 354, 435
MaeIII GTNAC 1 cut(s) 817
MalI GATC 2 cut(s) 495, 675
MboI GATC 2 cut(s) 493, 673
MboII GAAGA 6 cut(s) 49, 192, 195, 252, 680, 766
MhlI GDGCHC 2 cut(s) 360, 447
MlsI TGGCCA 1 cut(s) 645
MluCI AATT 3 cut(s) 103, 121, 734
MluNI TGGCCA 1 cut(s) 645
Mly113I GGCGCC 1 cut(s) 302
MlyI GAGTC 1 cut(s) 171
Mox20I TGGCCA 1 cut(s) 645
MroNI GCCGGC 1 cut(s) 304
MscI TGGCCA 1 cut(s) 645
MseI TTAA 1 cut(s) 384
MslI CAYNNNNRTG 1 cut(s) 603
Msp20I TGGCCA 1 cut(s) 645
MspI CCGG 1 cut(s) 305
MspR9I CCNGG 1 cut(s) 642
Mva1269I GAATGC 2 cut(s) 138, 576
MvaI CCWGG 1 cut(s) 642
MvnI CGCG 2 cut(s) 74, 552
NaeI GCCGGC 1 cut(s) 306
NarI GGCGCC 1 cut(s) 302
NdeII GATC 2 cut(s) 493, 673
NgoMIV GCCGGC 1 cut(s) 304
NlaIII CATG 4 cut(s) 401, 550, 602, 794
NlaIV GGNNCC 1 cut(s) 303
NmeAIII GCCGAG 3 cut(s) 203, 401, 564
NspI RCATGY 2 cut(s) 550, 794
PcsI WCGNNNNNNNCGW 1 cut(s) 444
PctI GAATGC 2 cut(s) 138, 576
PdiI GCCGGC 1 cut(s) 306
PfeI GAWTC 3 cut(s) 410, 712, 764
PleI GAGTC 1 cut(s) 171
PluTI GGCGCC 1 cut(s) 305
PpsI GAGTC 1 cut(s) 171
Psp6I CCWGG 1 cut(s) 640
PspEI GGTNACC 1 cut(s) 817
PspGI CCWGG 1 cut(s) 640
PspN4I GGNNCC 1 cut(s) 303
PstI CTGCAG 2 cut(s) 467, 811
RseI CAYNNNNRTG 1 cut(s) 603
SalI GTCGAC 1 cut(s) 290
SaqAI TTAA 1 cut(s) 384
Sau3AI GATC 2 cut(s) 493, 673
SchI GAGTC 1 cut(s) 171
ScrFI CCNGG 1 cut(s) 642
SduI GDGCHC 2 cut(s) 360, 447
SfaNI GCATC 4 cut(s) 48, 268, 334, 580
SfcI CTRYAG 2 cut(s) 463, 807
SfoI GGCGCC 1 cut(s) 303
SmiMI CAYNNNNRTG 1 cut(s) 603
Sse9I AATT 3 cut(s) 103, 121, 734
SsiI CCGC 9 cut(s) 79, 108, 136, 145, 216, 219, 299, 552, 799
SspDI GGCGCC 1 cut(s) 301
StyD4I CCNGG 1 cut(s) 640
TaiI ACGT 2 cut(s) 357, 438
TaqI TCGA 3 cut(s) 291, 321, 360
TaqII GACCGA 1 cut(s) 773
TasI AATT 3 cut(s) 103, 121, 734
TauI GCSGC 3 cut(s) 147, 221, 302
TfiI GAWTC 3 cut(s) 410, 712, 764
Tru1I TTAA 1 cut(s) 384
Tru9I TTAA 1 cut(s) 384
TscAI CASTG 3 cut(s) 49, 276, 472
TseI GCWGC 7 cut(s) 5, 30, 182, 248, 281, 347, 581
TspDTI ATGAA 1 cut(s) 339
TspRI CASTG 3 cut(s) 49, 276, 472
XceI RCATGY 2 cut(s) 550, 794
XmiI GTMKAC 1 cut(s) 291
ZraI GACGTC 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.