Rh2BG000400

Aminoacyl tRNA synthase complex-interacting multifunctional protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0014048
Physical Location & Seq
Forward (+)
60558 .. 64443
3886 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG000400.1

Sequence Viewer

Length: 759 bp
ATGGCAGCGAACGTTTTGAAATGCGGCACGGGCATAGGCGCGCGTCTGATATTCCTATCTCCGTCTCCTAAATCTTCACGGCCCTTTGGGCATTCACTTGCTCATTTCCACCAAATCACTGCGAATAGTTATCGTAGTATTTGTAGCAAACCGAAAGTTTGTGATTCTGCTGCTGATGCATCATCACCATCGGCAAGAAGAAGAAGCGTGGTCTCTTTTTGCGCCGCCACAGATGTTGAGGAGGCACAGGTAGAGGCAGAGGCAGAGGCGATAAGCGATGGTGATGAGGAACAGAAAAAGATAAAGGATGCAGCCGACCTGCTTGACATAAGGGTTGGGCTAGTCCTTAGGGCATGGAGGCATGAGGAAGCTGATTCTCTTTACGTGGAAGAGGTTGACATCGGAGAGCCTGAACCCAGAATCATCTGCAGTGGGCTTGTCAATTACATTCCTATTGATCACATTCAGGAGAGAAAAGTAGTTGTCCTTGCTAATCTGAAGCCCAGGAACATGCGGGGTGTCAAGTCTTGTGGAATGCTTATGGCCGCTTCTGATGCTTCCCATGAGAATGTTGAGCTTCTTGTGCCTCCTGAGGGTTCACTTCCTGGCCAAAGGATATGGTTTGGCTCTGAAGATGATCATCAAAATCAGCCTCCTCCTGCCACACCTAACCAGGTACTGTTTGCCTTCCCTATTTCCAAAAATACGGATACTTCTTTTCTCACCCTTCTGTATTTGAAAACGATTCTTGAAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_bind

Protein Analysis

252

Amino Acids

27.55

Weight (kDa)

5.41

Isoelectric Point (pI)

44.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_bind PF01588 110 - 204 9.1e-29 Putative tRNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014048)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59980
fragaria_vesca FvH4_1g00030 FvH4_1g00030 FvH4_1g00030
malus_domestica MD03G1140600.v1.1 MD11G1171300.v1.1
prunus_persica Prupe.6G126300_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0084251
rosa_laevigata RLG00000015603
rosa_multiflora Rmu_sc0003129.1_g000017
rosa_roxburghii Rroxscaffold_2G00156210
rosa_rugosa Rorug01G0452100
rosa_samantha Rh2AG000500 Rh2BG000400 Rh2CG000300 Rh2DG000500
rosa_wichuraiana Rw2G000030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 327
AccII CGCG 2 cut(s) 41, 43
AciI CCGC 4 cut(s) 24, 225, 514, 546
AclI AACGTT 1 cut(s) 12
AcoI YGGCCR 2 cut(s) 543, 607
AcsI RAATTY 1 cut(s) 753
AcuI CTGAAG 2 cut(s) 518, 651
AfaI GTAC 1 cut(s) 678
AfiI CCNNNNNNNGG 1 cut(s) 593
AgsI TTSAA 3 cut(s) 19, 739, 752
AjnI CCWGG 3 cut(s) 503, 604, 672
AluBI AGCT 2 cut(s) 371, 577
AluI AGCT 2 cut(s) 371, 577
Alw26I GTCTC 2 cut(s) 69, 217
AlwNI CAGNNNCTG 1 cut(s) 679
AoxI GGCC 3 cut(s) 80, 543, 607
ApeKI GCWGC 3 cut(s) 5, 170, 311
ApoI RAATTY 1 cut(s) 753
AspLEI GCGC 3 cut(s) 41, 43, 224
AspS9I GGNCC 1 cut(s) 81
AsuHPI GGTGA 3 cut(s) 177, 293, 715
AxyI CCTNAGG 2 cut(s) 347, 591
BalI TGGCCA 1 cut(s) 609
BbvI GCAGC 3 cut(s) 17, 157, 323
BccI CCATC 2 cut(s) 196, 272
BceAI ACGGC 1 cut(s) 95
BciT130I CCWGG 3 cut(s) 505, 606, 674
BciVI GTATCC 1 cut(s) 703
BclI TGATCA 2 cut(s) 457, 637
BcoDI GTCTC 2 cut(s) 69, 217
BfaI CTAG 1 cut(s) 341
BfmI CTRYAG 1 cut(s) 427
BfuAI ACCTGC 1 cut(s) 327
BfuI GTATCC 1 cut(s) 703
BglI GCCNNNNNGGC 1 cut(s) 88
BisI GCNGC 6 cut(s) 6, 25, 171, 225, 312, 546
BlsI GCNGC 6 cut(s) 7, 26, 172, 226, 313, 547
Bme1390I CCNGG 3 cut(s) 505, 606, 674
BmgT120I GGNCC 1 cut(s) 81
BmrFI CCNGG 3 cut(s) 505, 606, 674
BmsI GCATC 4 cut(s) 166, 188, 298, 544
BsaAI YACGTR 1 cut(s) 385
BsaBI GATNNNNATC 1 cut(s) 639
BsaI GGTCTC 1 cut(s) 217
BsaJI CCNNGG 1 cut(s) 503
Bsc4I CCNNNNNNNGG 1 cut(s) 593
Bse21I CCTNAGG 2 cut(s) 347, 591
Bse8I GATNNNNATC 1 cut(s) 639
BseBI CCWGG 3 cut(s) 505, 606, 674
BseDI CCNNGG 1 cut(s) 503
BseGI GGATG 1 cut(s) 313
BseJI GATNNNNATC 1 cut(s) 639
BseLI CCNNNNNNNGG 1 cut(s) 593
BseMII CTCAG 1 cut(s) 582
BsePI GCGCGC 1 cut(s) 39
BseRI GAGGAG 2 cut(s) 254, 645
BseXI GCAGC 3 cut(s) 17, 157, 323
Bsh1236I CGCG 2 cut(s) 41, 43
BshFI GGCC 3 cut(s) 82, 545, 609
BslI CCNNNNNNNGG 1 cut(s) 593
BsmAI GTCTC 2 cut(s) 69, 217
BsmBI CGTCTC 1 cut(s) 69
BsmI GAATGC 2 cut(s) 91, 540
BsnI GGCC 3 cut(s) 82, 545, 609
Bso31I GGTCTC 1 cut(s) 217
Bsp143I GATC 2 cut(s) 457, 637
BspACI CCGC 4 cut(s) 24, 225, 514, 546
BspANI GGCC 3 cut(s) 82, 545, 609
BspCNI CTCAG 1 cut(s) 583
BspFNI CGCG 2 cut(s) 41, 43
BspMAI CTGCAG 1 cut(s) 431
BspMI ACCTGC 1 cut(s) 327
BspTNI GGTCTC 1 cut(s) 217
BssECI CCNNGG 1 cut(s) 503
BssHII GCGCGC 1 cut(s) 39
BssMI GATC 2 cut(s) 457, 637
Bst2UI CCWGG 3 cut(s) 505, 606, 674
Bst4CI ACNGT 1 cut(s) 681
Bst6I CTCTTC 1 cut(s) 384
BstBAI YACGTR 1 cut(s) 385
BstC8I GCNNGC 1 cut(s) 41
BstDEI CTNAG 2 cut(s) 347, 591
BstF5I GGATG 1 cut(s) 313
BstFNI CGCG 2 cut(s) 41, 43
BstHHI GCGC 3 cut(s) 41, 43, 224
BstKTI GATC 2 cut(s) 460, 640
BstMAI GTCTC 2 cut(s) 69, 217
BstMBI GATC 2 cut(s) 457, 637
BstMWI GCNNNNNNNGC 5 cut(s) 30, 88, 176, 554, 583
BstNI CCWGG 3 cut(s) 505, 606, 674
BstNSI RCATGY 1 cut(s) 514
BstSCI CCNGG 3 cut(s) 503, 604, 672
BstSFI CTRYAG 1 cut(s) 427
BstUI CGCG 2 cut(s) 41, 43
BstV1I GCAGC 3 cut(s) 17, 157, 323
Bsu36I CCTNAGG 2 cut(s) 347, 591
BsuI GTATCC 1 cut(s) 703
BsuRI GGCC 3 cut(s) 82, 545, 609
BtgZI GCGATG 1 cut(s) 291
BtsCI GGATG 1 cut(s) 313
BtsI GCAGTG 2 cut(s) 117, 436
BtsIMutI CAGTG 2 cut(s) 117, 436
BveI ACCTGC 1 cut(s) 327
Cac8I GCNNGC 1 cut(s) 41
CaiI CAGNNNCTG 1 cut(s) 679
CfoI GCGC 3 cut(s) 41, 43, 224
Cfr13I GGNCC 1 cut(s) 81
CseI GACGC 1 cut(s) 32
CsiI ACCWGGT 1 cut(s) 672
Csp6I GTAC 1 cut(s) 677
CspCI CAANNNNNGTGG 4 cut(s) 217, 252, 511, 546
CviAII CATG 4 cut(s) 354, 362, 511, 563
CviQI GTAC 1 cut(s) 677
DdeI CTNAG 2 cut(s) 347, 591
DpnI GATC 2 cut(s) 459, 639
DpnII GATC 2 cut(s) 457, 637
EaeI YGGCCR 2 cut(s) 543, 607
Eam1104I CTCTTC 1 cut(s) 384
EarI CTCTTC 1 cut(s) 384
Eco31I GGTCTC 1 cut(s) 217
Eco57I CTGAAG 2 cut(s) 518, 651
Eco81I CCTNAGG 2 cut(s) 347, 591
EcoRII CCWGG 3 cut(s) 503, 604, 672
EcoT22I ATGCAT 1 cut(s) 181
Esp3I CGTCTC 1 cut(s) 69
FaeI CATG 4 cut(s) 357, 365, 514, 566
FaiI YATR 8 cut(s) 35, 329, 355, 363, 512, 542, 564, 619
FatI CATG 4 cut(s) 353, 361, 510, 562
FauI CCCGC 1 cut(s) 507
FbaI TGATCA 2 cut(s) 457, 637
Fnu4HI GCNGC 6 cut(s) 6, 25, 171, 225, 312, 546
FokI GGATG 1 cut(s) 320
Fsp4HI GCNGC 6 cut(s) 6, 25, 171, 225, 312, 546
FspBI CTAG 1 cut(s) 341
GlaI GCGC 3 cut(s) 40, 42, 223
GluI GCNGC 6 cut(s) 6, 25, 171, 225, 312, 546
HaeIII GGCC 3 cut(s) 82, 545, 609
HgaI GACGC 1 cut(s) 32
HhaI GCGC 3 cut(s) 41, 43, 224
Hin1II CATG 4 cut(s) 357, 365, 514, 566
Hin6I GCGC 3 cut(s) 39, 41, 222
HinP1I GCGC 3 cut(s) 39, 41, 222
HincII GTYRAC 1 cut(s) 397
HindII GTYRAC 1 cut(s) 397
HinfI GANTC 4 cut(s) 164, 374, 420, 745
HphI GGTGA 3 cut(s) 177, 293, 715
Hpy166II GTNNAC 2 cut(s) 397, 599
Hpy188I TCNGA 5 cut(s) 48, 404, 498, 553, 631
Hpy188III TCNNGA 3 cut(s) 467, 590, 749
Hpy8I GTNNAC 2 cut(s) 397, 599
HpyAV CCTTC 2 cut(s) 697, 737
HpyCH4III ACNGT 1 cut(s) 681
HpyCH4IV ACGT 2 cut(s) 12, 384
HpyCH4V TGCA 3 cut(s) 179, 311, 429
HpyF10VI GCNNNNNNNGC 5 cut(s) 30, 88, 176, 554, 583
HpyF3I CTNAG 2 cut(s) 347, 591
HpySE526I ACGT 2 cut(s) 12, 384
Hsp92II CATG 4 cut(s) 357, 365, 514, 566
HspAI GCGC 3 cut(s) 39, 41, 222
Ksp22I TGATCA 2 cut(s) 457, 637
Kzo9I GATC 2 cut(s) 457, 637
Lsp1109I GCAGC 3 cut(s) 17, 157, 323
LweI GCATC 4 cut(s) 166, 188, 298, 544
MabI ACCWGGT 1 cut(s) 672
MaeI CTAG 1 cut(s) 341
MaeII ACGT 2 cut(s) 12, 384
MalI GATC 2 cut(s) 459, 639
MboI GATC 2 cut(s) 457, 637
MboII GAAGA 5 cut(s) 66, 210, 213, 401, 644
MlsI TGGCCA 1 cut(s) 609
MluCI AATT 2 cut(s) 442, 753
MluNI TGGCCA 1 cut(s) 609
Mox20I TGGCCA 1 cut(s) 609
Mph1103I ATGCAT 1 cut(s) 181
MscI TGGCCA 1 cut(s) 609
MslI CAYNNNNRTG 1 cut(s) 567
Msp20I TGGCCA 1 cut(s) 609
MspR9I CCNGG 3 cut(s) 505, 606, 674
Mva1269I GAATGC 2 cut(s) 91, 540
MvaI CCWGG 3 cut(s) 505, 606, 674
MvnI CGCG 2 cut(s) 41, 43
MwoI GCNNNNNNNGC 5 cut(s) 30, 88, 176, 554, 583
NdeII GATC 2 cut(s) 457, 637
NlaIII CATG 4 cut(s) 357, 365, 514, 566
NsiI ATGCAT 1 cut(s) 181
NspI RCATGY 1 cut(s) 514
PauI GCGCGC 1 cut(s) 39
PctI GAATGC 2 cut(s) 91, 540
PfeI GAWTC 4 cut(s) 164, 374, 420, 745
PkrI GCNGC 6 cut(s) 7, 26, 172, 226, 313, 547
Ppu21I YACGTR 1 cut(s) 385
Psp1406I AACGTT 1 cut(s) 12
Psp6I CCWGG 3 cut(s) 503, 604, 672
PspGI CCWGG 3 cut(s) 503, 604, 672
PspPI GGNCC 1 cut(s) 81
PstI CTGCAG 1 cut(s) 431
PstNI CAGNNNCTG 1 cut(s) 679
PteI GCGCGC 1 cut(s) 39
RsaI GTAC 1 cut(s) 678
RsaNI GTAC 1 cut(s) 677
RseI CAYNNNNRTG 1 cut(s) 567
SatI GCNGC 6 cut(s) 6, 25, 171, 225, 312, 546
Sau3AI GATC 2 cut(s) 457, 637
Sau96I GGNCC 1 cut(s) 81
ScrFI CCNGG 3 cut(s) 505, 606, 674
SetI ASST 9 cut(s) 15, 252, 321, 373, 387, 396, 579, 670, 678
SexAI ACCWGGT 1 cut(s) 672
SfaNI GCATC 4 cut(s) 166, 188, 298, 544
SfcI CTRYAG 1 cut(s) 427
SmiMI CAYNNNNRTG 1 cut(s) 567
Sse9I AATT 2 cut(s) 442, 753
SsiI CCGC 4 cut(s) 24, 225, 514, 546
SspMI CTAG 1 cut(s) 341
StyD4I CCNGG 3 cut(s) 503, 604, 672
TaaI ACNGT 1 cut(s) 681
TaiI ACGT 2 cut(s) 15, 387
TasI AATT 2 cut(s) 442, 753
TauI GCSGC 3 cut(s) 27, 227, 548
TfiI GAWTC 4 cut(s) 164, 374, 420, 745
TscAI CASTG 2 cut(s) 124, 436
TseI GCWGC 3 cut(s) 5, 170, 311
TspGWI ACGGA 2 cut(s) 51, 722
TspRI CASTG 2 cut(s) 124, 436
XapI RAATTY 1 cut(s) 753
XceI RCATGY 1 cut(s) 514
XspI CTAG 1 cut(s) 341
Zsp2I ATGCAT 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.