AT3G60720

cysteine-rich repeat secretory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0010300
Physical Location & Seq
Forward (+)
22441771 .. 22443876
2106 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G60720.2

Sequence Viewer

Length: 840 bp
ATGAGAAGACTCTTTCTCTTCTCTCTCCTCTTTTTATTCTTTTACTCATCCTCCTCCTCAAGATCTTCATCAGAATCTCACATCTTCATCTACGGAGGATGTTCACCGGAAAAATACACACCAAACACACCTTTTGAGTCAAACCGCGACACTTTCCTCTCCTCTGTCGTCACTTCCTCCTCTGACGCGTCCTTCAACAGCTTTGCCGTCGGCAACGACTCCTCCTCCTCCTCATCCTCTTCCGCCGTCTTCGGCCTCTATCAATGCCGTGACGATCTCCGATCATCAGACTGCTCAAAATGCATCCAAACCTCAGTCGATCAAATCACTCTCATTTGTCCTTACTCTTACGGCGCTTCTCTCCAGCTCGAAGGCTGCTTCTTACGTTACGAAACAAACGATTTTCTAGGGAAACCAGACACAAGTCTTAGGTACAAGAAGTGTAGTTCCAAGAGCGTTGAGAATGACTACGATTTCTTTAAGCGGAGAGACGATGTGTTGTCGGATCTTGAGTCGACTCAACTCGGTTACAAAGTTAGCCGGTCCGGTTTAGTCGAAGGTTATGCTCAGTGTGTTGGTGACTTGAGTCCTAGTGACTGTACGGCTTGTCTTGCCGAGTCTGTCGGAAAATTAAAGAATCTTTGTGGCTCGGCGGTTGCAGCTGAGGTTTACTTGGCTCAGTGCTATGCTCGTTATTGGGGCTCTGGTTACTATGACTTCTCTTCAGATCCAACGAATGGAGATCACGTGGGGAAATCAATCGCGATCATCGTAGGAGTCATTGCTGGATTCGCAATTCTTGTTGTTCTCCTCTCCCTTTGTAGAAACTCCATGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stress-antifung

Protein Analysis

279

Amino Acids

30.53

Weight (kDa)

5.25

Isoelectric Point (pI)

46.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 34 - 132 2.8e-19 Salt stress response/antifungal
Stress-antifung PF01657 148 - 232 4e-11 Salt stress response/antifungal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010300)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G60720 AT3G60720 AT3G60720
fragaria_vesca FvH4_7g11310 FvH4_7g11310
malus_domestica MD02G1208100.v1.1 MD07G1117900.v1.1
prunus_persica Prupe.2G140900_v2.0.a1
pyrus_communis pycom02g17030 pycom07g10630
rosa_chinensis RchiOBHm_Chr1g0349511
rosa_laevigata RLG00000028545
rosa_multiflora Rmu_co8400581.1_g000001 Rmu_sc0002495.1_g000015
rosa_roxburghii Rroxscaffold_4G00305500
rosa_rugosa Rorug01G0204200
rosa_samantha Rh1AG221500 Rh1BG187600 Rh1CG206400 Rh1DG216900
rosa_wichuraiana Rw1G018830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 737
AccI GTMKAC 1 cut(s) 515
AccII CGCG 3 cut(s) 147, 188, 764
AciI CCGC 4 cut(s) 145, 243, 484, 653
AclWI GGATC 2 cut(s) 513, 722
AcuI CTGAAG 1 cut(s) 708
AcvI CACGTG 1 cut(s) 748
AfaI GTAC 2 cut(s) 434, 601
AfiI CCNNNNNNNGG 1 cut(s) 737
AflIII ACRYGT 1 cut(s) 186
AgsI TTSAA 1 cut(s) 196
AluBI AGCT 3 cut(s) 201, 367, 662
AluI AGCT 3 cut(s) 201, 367, 662
Alw26I GTCTC 1 cut(s) 483
AlwI GGATC 2 cut(s) 513, 722
AoxI GGCC 1 cut(s) 253
ApeKI GCWGC 2 cut(s) 375, 659
AspLEI GCGC 1 cut(s) 356
AspS9I GGNCC 1 cut(s) 543
AsuHPI GGTGA 2 cut(s) 96, 590
AvaII GGWCC 1 cut(s) 543
BanII GRGCYC 1 cut(s) 704
BbrPI CACGTG 1 cut(s) 748
BbsI GAAGAC 2 cut(s) 13, 241
BbvCI CCTCAGC 1 cut(s) 663
BbvI GCAGC 2 cut(s) 362, 671
BceAI ACGGC 5 cut(s) 191, 230, 252, 367, 618
BcgI CGANNNNNNTGC 2 cut(s) 545, 579
BcoDI GTCTC 1 cut(s) 483
BfaI CTAG 2 cut(s) 407, 591
BfoI RGCGCY 1 cut(s) 357
BglII AGATCT 1 cut(s) 62
BisI GCNGC 2 cut(s) 376, 660
BlsI GCNGC 2 cut(s) 377, 661
Bme18I GGWCC 1 cut(s) 543
BmgT120I GGNCC 1 cut(s) 543
BmsI GCATC 1 cut(s) 312
BoxI GACNNNNGTC 2 cut(s) 423, 585
BpiI GAAGAC 2 cut(s) 13, 241
BpmI CTGGAG 1 cut(s) 347
Bpu10I CCTNAGC 1 cut(s) 663
BpuEI CTTGAG 3 cut(s) 43, 530, 604
BsaAI YACGTR 1 cut(s) 748
BsaBI GATNNNNATC 1 cut(s) 67
BsaWI WCCGGW 2 cut(s) 106, 545
BsaXI ACNNNNNCTCC 8 cut(s) 35, 65, 206, 209, 236, 239, 732, 762
Bsc4I CCNNNNNNNGG 1 cut(s) 737
Bse118I RCCGGY 1 cut(s) 540
Bse3DI GCAATG 1 cut(s) 780
Bse8I GATNNNNATC 1 cut(s) 67
BseGI GGATG 4 cut(s) 47, 104, 233, 303
BseJI GATNNNNATC 1 cut(s) 67
BseLI CCNNNNNNNGG 1 cut(s) 737
BseMI GCAATG 1 cut(s) 780
BseMII CTCAG 4 cut(s) 327, 581, 654, 692
BseXI GCAGC 2 cut(s) 362, 671
Bsh1236I CGCG 3 cut(s) 147, 188, 764
BshFI GGCC 1 cut(s) 255
BsiSI CCGG 3 cut(s) 107, 541, 546
BslI CCNNNNNNNGG 1 cut(s) 737
BsmAI GTCTC 1 cut(s) 483
BsmBI CGTCTC 1 cut(s) 483
BsnI GGCC 1 cut(s) 255
Bsp1286I GDGCHC 1 cut(s) 704
Bsp143I GATC 8 cut(s) 62, 274, 281, 319, 505, 727, 742, 765
Bsp68I TCGCGA 1 cut(s) 764
BspACI CCGC 4 cut(s) 145, 243, 484, 653
BspANI GGCC 1 cut(s) 255
BspCNI CTCAG 4 cut(s) 326, 580, 655, 691
BspFNI CGCG 3 cut(s) 147, 188, 764
BspPI GGATC 2 cut(s) 513, 722
BsrDI GCAATG 1 cut(s) 780
BsrFI RCCGGY 1 cut(s) 540
BssAI RCCGGY 1 cut(s) 540
BssMI GATC 8 cut(s) 62, 274, 281, 319, 505, 727, 742, 765
Bst4CI ACNGT 1 cut(s) 599
Bst6I CTCTTC 3 cut(s) 23, 244, 727
BstBAI YACGTR 1 cut(s) 748
BstDEI CTNAG 5 cut(s) 313, 428, 567, 663, 678
BstF5I GGATG 4 cut(s) 47, 104, 233, 303
BstFNI CGCG 3 cut(s) 147, 188, 764
BstH2I RGCGCY 1 cut(s) 357
BstHHI GCGC 1 cut(s) 356
BstKTI GATC 8 cut(s) 65, 277, 284, 322, 508, 730, 745, 768
BstMAI GTCTC 1 cut(s) 483
BstMBI GATC 8 cut(s) 62, 274, 281, 319, 505, 727, 742, 765
BstMWI GCNNNNNNNGC 4 cut(s) 300, 611, 659, 791
BstPAI GACNNNNGTC 2 cut(s) 423, 585
BstUI CGCG 3 cut(s) 147, 188, 764
BstV1I GCAGC 2 cut(s) 362, 671
BstV2I GAAGAC 2 cut(s) 13, 241
BstX2I RGATCY 3 cut(s) 62, 505, 727
BstYI RGATCY 3 cut(s) 62, 505, 727
BsuRI GGCC 1 cut(s) 255
BtsCI GGATG 4 cut(s) 47, 104, 233, 303
BtsIMutI CAGTG 2 cut(s) 575, 686
BtuMI TCGCGA 1 cut(s) 764
CfoI GCGC 1 cut(s) 356
Cfr10I RCCGGY 1 cut(s) 540
Cfr13I GGNCC 1 cut(s) 543
CpoI CGGWCCG 1 cut(s) 543
CseI GACGC 2 cut(s) 177, 194
Csp6I GTAC 2 cut(s) 433, 600
CspI CGGWCCG 1 cut(s) 543
CviAII CATG 1 cut(s) 832
CviQI GTAC 2 cut(s) 433, 600
DdeI CTNAG 5 cut(s) 313, 428, 567, 663, 678
DpnI GATC 8 cut(s) 64, 276, 283, 321, 507, 729, 744, 767
DpnII GATC 8 cut(s) 62, 274, 281, 319, 505, 727, 742, 765
Eam1104I CTCTTC 3 cut(s) 23, 244, 727
EarI CTCTTC 3 cut(s) 23, 244, 727
EciI GGCGGA 1 cut(s) 232
Eco24I GRGCYC 1 cut(s) 704
Eco47I GGWCC 1 cut(s) 543
Eco57I CTGAAG 1 cut(s) 708
Eco72I CACGTG 1 cut(s) 748
EcoT22I ATGCAT 2 cut(s) 305, 837
EcoT38I GRGCYC 1 cut(s) 704
Esp3I CGTCTC 1 cut(s) 483
FaeI CATG 1 cut(s) 835
FaiI YATR 4 cut(s) 564, 687, 714, 833
FatI CATG 1 cut(s) 831
FblI GTMKAC 1 cut(s) 515
Fnu4HI GCNGC 2 cut(s) 376, 660
FokI GGATG 4 cut(s) 34, 111, 220, 290
FriOI GRGCYC 1 cut(s) 704
Fsp4HI GCNGC 2 cut(s) 376, 660
FspBI CTAG 2 cut(s) 407, 591
GlaI GCGC 1 cut(s) 355
GluI GCNGC 2 cut(s) 376, 660
GsuI CTGGAG 1 cut(s) 347
HaeII RGCGCY 1 cut(s) 357
HaeIII GGCC 1 cut(s) 255
HapII CCGG 3 cut(s) 107, 541, 546
HgaI GACGC 2 cut(s) 177, 194
HhaI GCGC 1 cut(s) 356
Hin1II CATG 1 cut(s) 835
Hin6I GCGC 1 cut(s) 354
HinP1I GCGC 1 cut(s) 354
HincII GTYRAC 1 cut(s) 516
HindII GTYRAC 1 cut(s) 516
HpaII CCGG 3 cut(s) 107, 541, 546
HphI GGTGA 2 cut(s) 96, 590
Hpy166II GTNNAC 3 cut(s) 104, 516, 670
Hpy188I TCNGA 7 cut(s) 73, 184, 281, 289, 505, 626, 727
Hpy188III TCNNGA 3 cut(s) 60, 509, 763
Hpy8I GTNNAC 3 cut(s) 104, 516, 670
Hpy99I CGWCG 1 cut(s) 212
HpyAV CCTTC 3 cut(s) 202, 365, 551
HpyCH4III ACNGT 1 cut(s) 599
HpyCH4IV ACGT 2 cut(s) 385, 747
HpyCH4V TGCA 3 cut(s) 303, 659, 835
HpyF10VI GCNNNNNNNGC 4 cut(s) 300, 611, 659, 791
HpyF3I CTNAG 5 cut(s) 313, 428, 567, 663, 678
HpySE526I ACGT 2 cut(s) 385, 747
Hsp92II CATG 1 cut(s) 835
HspAI GCGC 1 cut(s) 354
Kzo9I GATC 8 cut(s) 62, 274, 281, 319, 505, 727, 742, 765
LpnPI CCDG 7 cut(s) 120, 377, 429, 554, 559, 690, 771
Lsp1109I GCAGC 2 cut(s) 362, 671
LweI GCATC 1 cut(s) 312
MaeI CTAG 2 cut(s) 407, 591
MaeII ACGT 2 cut(s) 385, 747
MaeIII GTNAC 7 cut(s) 169, 269, 386, 527, 578, 593, 707
MalI GATC 8 cut(s) 64, 276, 283, 321, 507, 729, 744, 767
MboI GATC 8 cut(s) 62, 274, 281, 319, 505, 727, 742, 765
MboII GAAGA 7 cut(s) 10, 18, 57, 76, 231, 241, 714
MflI RGATCY 3 cut(s) 62, 505, 727
MhlI GDGCHC 1 cut(s) 704
MluCI AATT 2 cut(s) 629, 795
MluI ACGCGT 1 cut(s) 186
MlyI GAGTC 8 cut(s) 3, 146, 212, 511, 521, 595, 626, 786
MmeI TCCRAC 3 cut(s) 483, 604, 755
Mph1103I ATGCAT 2 cut(s) 305, 837
MseI TTAA 2 cut(s) 480, 632
MspA1I CMGCKG 1 cut(s) 662
MspI CCGG 3 cut(s) 107, 541, 546
MvnI CGCG 3 cut(s) 147, 188, 764
MwoI GCNNNNNNNGC 4 cut(s) 300, 611, 659, 791
NdeII GATC 8 cut(s) 62, 274, 281, 319, 505, 727, 742, 765
NlaIII CATG 1 cut(s) 835
NmeAIII GCCGAG 2 cut(s) 629, 640
NmuCI GTSAC 4 cut(s) 169, 269, 578, 593
NruI TCGCGA 1 cut(s) 764
NsiI ATGCAT 2 cut(s) 305, 837
PcsI WCGNNNNNNNCGW 2 cut(s) 396, 768
PfeI GAWTC 3 cut(s) 74, 637, 789
PflMI CCANNNNNTGG 1 cut(s) 737
PkrI GCNGC 2 cut(s) 377, 661
PleI GAGTC 8 cut(s) 3, 145, 212, 511, 520, 594, 625, 785
PmaCI CACGTG 1 cut(s) 748
PmlI CACGTG 1 cut(s) 748
PpsI GAGTC 8 cut(s) 3, 145, 212, 511, 520, 594, 625, 785
Ppu21I YACGTR 1 cut(s) 748
PshAI GACNNNNGTC 2 cut(s) 423, 585
PspCI CACGTG 1 cut(s) 748
PspPI GGNCC 1 cut(s) 543
PsuI RGATCY 3 cut(s) 62, 505, 727
PvuII CAGCTG 1 cut(s) 662
RruI TCGCGA 1 cut(s) 764
RsaI GTAC 2 cut(s) 434, 601
RsaNI GTAC 2 cut(s) 433, 600
Rsr2I CGGWCCG 1 cut(s) 543
RsrII CGGWCCG 1 cut(s) 543
SalI GTCGAC 1 cut(s) 514
SaqAI TTAA 2 cut(s) 480, 632
SatI GCNGC 2 cut(s) 376, 660
Sau3AI GATC 8 cut(s) 62, 274, 281, 319, 505, 727, 742, 765
Sau96I GGNCC 1 cut(s) 543
SchI GAGTC 8 cut(s) 3, 146, 212, 511, 521, 595, 626, 786
SduI GDGCHC 1 cut(s) 704
SfaNI GCATC 1 cut(s) 312
SinI GGWCC 1 cut(s) 543
SmlI CTYRAG 3 cut(s) 58, 509, 583
SmoI CTYRAG 3 cut(s) 58, 509, 583
Sse9I AATT 2 cut(s) 629, 795
SsiI CCGC 4 cut(s) 145, 243, 484, 653
SspMI CTAG 2 cut(s) 407, 591
TaaI ACNGT 1 cut(s) 599
TaiI ACGT 2 cut(s) 388, 750
TaqI TCGA 4 cut(s) 318, 369, 515, 555
TasI AATT 2 cut(s) 629, 795
TfiI GAWTC 3 cut(s) 74, 637, 789
Tru1I TTAA 2 cut(s) 480, 632
Tru9I TTAA 2 cut(s) 480, 632
TscAI CASTG 2 cut(s) 575, 686
TseFI GTSAC 4 cut(s) 169, 269, 578, 593
TseI GCWGC 2 cut(s) 375, 659
Tsp45I GTSAC 4 cut(s) 169, 269, 578, 593
TspDTI ATGAA 2 cut(s) 57, 76
TspGWI ACGGA 1 cut(s) 108
TspRI CASTG 2 cut(s) 575, 686
Van91I CCANNNNNTGG 1 cut(s) 737
VpaK11BI GGWCC 1 cut(s) 543
XmiI GTMKAC 1 cut(s) 515
XspI CTAG 2 cut(s) 407, 591
Zsp2I ATGCAT 2 cut(s) 305, 837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.