Rroxscaffold_4G00305500

Cysteine-rich repeat secretory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0010300
Physical Location & Seq
Reverse (-)
26176847 .. 26179189
2343 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00305500.1

Sequence Viewer

Length: 738 bp
ATGGCATCACCACCCCCCAACCCAAAGCCACAATACAAAAACAATAGAAATCCAAGTGATAGCCATATTGTTGTTAGCTCATCCTCTCAAGCCTCTTACAACGCCTTTTCTGTCGGAAACGGAACCTCAACATCACCAGAAGGTGCCATTTATGGCTTGTACCAGTGCAGAGGTGACTTAAAGATTTTTGACTGTTCGAAATGCATCGAAAGCGCGGTGAATCAAGTCACTCTAGTCTGTCCCTACTCTTATGGCGCCTCTTTGCAGTTACAAGGTTGTTATGTTAGATACGAGCATGCTGAGTTTTTGGGCAAGCTTGATACAAGTTTGAGGTACAAGAAATGTAGTAAGACTGTACATGAAGATGTTGAGTTCTTTCGGAGAAGAGATGACGTGCTAGCTGACTTGCAAGGGACTAGTGGATTTAGGGTTAGTAGTTCAGGCTTGGTTGAGGGTTTTGCGCAGTGTTTGGGGGATTTGAGCCAAAACGACTGCTCTTCTTGCGTCGCAGATGCTGTGGGAAAGCTGAAGAGTTTGTGTGGGTCAGCAGCTGCGGTAGATGTGTTCTTGGTTCAGTGTTATGCTAGGTACTGGGCTTCTGGCTACTACGATATCTCAGATTCCTCTAACGAAGACCAAGTGGGAAAGACTGTGGCCATTATCGTGGGGGTGGTAGCAAGTCTAGCTATTCTCATTGTTCTTCTATCAGTTTGCCGGAAAACCATGGGTCGTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stress-antifung

Protein Analysis

245

Amino Acids

26.5

Weight (kDa)

6.79

Isoelectric Point (pI)

32.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 9 - 99 2.3e-15 Salt stress response/antifungal
Stress-antifung PF01657 115 - 197 1.5e-10 Salt stress response/antifungal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010300)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G60720 AT3G60720 AT3G60720
fragaria_vesca FvH4_7g11310 FvH4_7g11310
malus_domestica MD02G1208100.v1.1 MD07G1117900.v1.1
prunus_persica Prupe.2G140900_v2.0.a1
pyrus_communis pycom02g17030 pycom07g10630
rosa_chinensis RchiOBHm_Chr1g0349511
rosa_laevigata RLG00000028545
rosa_multiflora Rmu_co8400581.1_g000001 Rmu_sc0002495.1_g000015
rosa_roxburghii Rroxscaffold_4G00305500
rosa_rugosa Rorug01G0204200
rosa_samantha Rh1AG221500 Rh1BG187600 Rh1CG206400 Rh1DG216900
rosa_wichuraiana Rw1G018830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 462
AccB1I GGYRCC 2 cut(s) 143, 254
AccII CGCG 1 cut(s) 215
AciI CCGC 2 cut(s) 215, 554
AcoI YGGCCR 1 cut(s) 654
AcuI CTGAAG 1 cut(s) 548
AcyI GRCGYC 1 cut(s) 255
AfaI GTAC 4 cut(s) 161, 335, 357, 590
AhlI ACTAGT 1 cut(s) 416
AjiI CACGTC 1 cut(s) 394
AluBI AGCT 6 cut(s) 78, 316, 401, 526, 551, 686
AluI AGCT 6 cut(s) 78, 316, 401, 526, 551, 686
AlwNI CAGNNNCTG 2 cut(s) 515, 551
AoxI GGCC 1 cut(s) 654
ApeKI GCWGC 2 cut(s) 548, 551
AspLEI GCGC 3 cut(s) 215, 257, 463
AsuHPI GGTGA 3 cut(s) 126, 185, 229
AsuII TTCGAA 1 cut(s) 197
AsuNHI GCTAGC 1 cut(s) 397
BalI TGGCCA 1 cut(s) 656
BanI GGYRCC 2 cut(s) 143, 254
BbsI GAAGAC 1 cut(s) 639
BbvI GCAGC 2 cut(s) 538, 560
BcuI ACTAGT 1 cut(s) 416
BfaI CTAG 5 cut(s) 233, 398, 417, 585, 683
BfoI RGCGCY 1 cut(s) 258
BisI GCNGC 2 cut(s) 549, 552
BlsI GCNGC 2 cut(s) 550, 553
BmgBI CACGTC 1 cut(s) 394
BmiI GGNNCC 3 cut(s) 124, 145, 256
BmrI ACTGGG 1 cut(s) 601
BmsI GCATC 3 cut(s) 14, 213, 502
BmtI GCTAGC 1 cut(s) 401
BmuI ACTGGG 1 cut(s) 601
BpiI GAAGAC 1 cut(s) 639
Bpu14I TTCGAA 1 cut(s) 197
BpuEI CTTGAG 1 cut(s) 72
BsaHI GRCGYC 1 cut(s) 255
BsaJI CCNNGG 1 cut(s) 723
Bse1I ACTGG 2 cut(s) 163, 596
BseDI CCNNGG 1 cut(s) 723
BseGI GGATG 1 cut(s) 80
BseMII CTCAG 2 cut(s) 291, 630
BseNI ACTGG 2 cut(s) 163, 596
BseXI GCAGC 2 cut(s) 538, 560
BsgI GTGCAG 1 cut(s) 187
Bsh1236I CGCG 1 cut(s) 215
BshFI GGCC 1 cut(s) 656
BshNI GGYRCC 2 cut(s) 143, 254
BsiSI CCGG 1 cut(s) 715
BslFI GGGAC 2 cut(s) 225, 427
BsmFI GGGAC 2 cut(s) 225, 427
BsnI GGCC 1 cut(s) 656
Bsp119I TTCGAA 1 cut(s) 197
Bsp1407I TGTACA 1 cut(s) 355
Bsp19I CCATGG 1 cut(s) 723
BspACI CCGC 2 cut(s) 215, 554
BspANI GGCC 1 cut(s) 656
BspCNI CTCAG 2 cut(s) 292, 629
BspFNI CGCG 1 cut(s) 215
BspLI GGNNCC 3 cut(s) 124, 145, 256
BspOI GCTAGC 1 cut(s) 401
BspQI GCTCTTC 1 cut(s) 502
BspT104I TTCGAA 1 cut(s) 197
BspT107I GGYRCC 2 cut(s) 143, 254
BsrGI TGTACA 1 cut(s) 355
BsrI ACTGG 2 cut(s) 163, 596
BssECI CCNNGG 1 cut(s) 723
BssNI GRCGYC 1 cut(s) 255
BssT1I CCWWGG 1 cut(s) 723
Bst4CI ACNGT 3 cut(s) 194, 355, 652
Bst6I CTCTTC 3 cut(s) 379, 502, 524
BstACI GRCGYC 1 cut(s) 255
BstAUI TGTACA 1 cut(s) 355
BstBI TTCGAA 1 cut(s) 197
BstC8I GCNNGC 3 cut(s) 297, 314, 399
BstDEI CTNAG 2 cut(s) 300, 616
BstDSI CCRYGG 1 cut(s) 723
BstF5I GGATG 1 cut(s) 80
BstFNI CGCG 1 cut(s) 215
BstH2I RGCGCY 1 cut(s) 258
BstHHI GCGC 3 cut(s) 215, 257, 463
BstMWI GCNNNNNNNGC 3 cut(s) 210, 501, 683
BstNSI RCATGY 1 cut(s) 299
BstUI CGCG 1 cut(s) 215
BstV1I GCAGC 2 cut(s) 538, 560
BstV2I GAAGAC 1 cut(s) 639
BstXI CCANNNNNNTGG 1 cut(s) 664
BsuRI GGCC 1 cut(s) 656
BtgI CCRYGG 1 cut(s) 723
BtrI CACGTC 1 cut(s) 394
BtsCI GGATG 1 cut(s) 80
BtsI GCAGTG 1 cut(s) 470
BtsIMutI CAGTG 3 cut(s) 170, 470, 581
Cac8I GCNNGC 3 cut(s) 297, 314, 399
CaiI CAGNNNCTG 2 cut(s) 515, 551
CfoI GCGC 3 cut(s) 215, 257, 463
CseI GACGC 1 cut(s) 493
Csp6I GTAC 4 cut(s) 160, 334, 356, 589
CviAII CATG 3 cut(s) 296, 359, 724
CviQI GTAC 4 cut(s) 160, 334, 356, 589
DdeI CTNAG 2 cut(s) 300, 616
DinI GGCGCC 1 cut(s) 256
EaeI YGGCCR 1 cut(s) 654
Eam1104I CTCTTC 3 cut(s) 379, 502, 524
EarI CTCTTC 3 cut(s) 379, 502, 524
Eco130I CCWWGG 1 cut(s) 723
Eco32I GATATC 1 cut(s) 613
Eco57I CTGAAG 1 cut(s) 548
EcoRV GATATC 1 cut(s) 613
EcoT14I CCWWGG 1 cut(s) 723
EcoT22I ATGCAT 1 cut(s) 206
EgeI GGCGCC 1 cut(s) 256
EheI GGCGCC 1 cut(s) 256
ErhI CCWWGG 1 cut(s) 723
FaeI CATG 3 cut(s) 299, 362, 727
FaiI YATR 8 cut(s) 66, 153, 252, 282, 297, 360, 582, 725
FaqI GGGAC 2 cut(s) 225, 427
FatI CATG 3 cut(s) 295, 358, 723
Fnu4HI GCNGC 2 cut(s) 549, 552
FokI GGATG 1 cut(s) 67
Fsp4HI GCNGC 2 cut(s) 549, 552
FspBI CTAG 5 cut(s) 233, 398, 417, 585, 683
FspI TGCGCA 1 cut(s) 462
GlaI GCGC 3 cut(s) 214, 256, 462
GluI GCNGC 2 cut(s) 549, 552
HaeII RGCGCY 1 cut(s) 258
HaeIII GGCC 1 cut(s) 656
HapII CCGG 1 cut(s) 715
HgaI GACGC 1 cut(s) 493
HhaI GCGC 3 cut(s) 215, 257, 463
Hin1I GRCGYC 1 cut(s) 255
Hin1II CATG 3 cut(s) 299, 362, 727
Hin6I GCGC 3 cut(s) 213, 255, 461
HinP1I GCGC 3 cut(s) 213, 255, 461
HindIII AAGCTT 1 cut(s) 314
HinfI GANTC 2 cut(s) 220, 620
HpaII CCGG 1 cut(s) 715
HphI GGTGA 3 cut(s) 126, 185, 229
Hpy188I TCNGA 3 cut(s) 116, 381, 619
Hpy99I CGWCG 1 cut(s) 509
HpyAV CCTTC 1 cut(s) 134
HpyCH4III ACNGT 3 cut(s) 194, 355, 652
HpyCH4IV ACGT 1 cut(s) 393
HpyCH4V TGCA 4 cut(s) 168, 204, 265, 409
HpyF10VI GCNNNNNNNGC 3 cut(s) 210, 501, 683
HpyF3I CTNAG 2 cut(s) 300, 616
HpySE526I ACGT 1 cut(s) 393
Hsp92I GRCGYC 1 cut(s) 255
Hsp92II CATG 3 cut(s) 299, 362, 727
HspAI GCGC 3 cut(s) 213, 255, 461
KasI GGCGCC 1 cut(s) 254
LguI GCTCTTC 1 cut(s) 502
LpnPI CCDG 6 cut(s) 150, 176, 426, 577, 585, 728
Lsp1109I GCAGC 2 cut(s) 538, 560
LweI GCATC 3 cut(s) 14, 213, 502
MaeI CTAG 5 cut(s) 233, 398, 417, 585, 683
MaeII ACGT 1 cut(s) 393
MaeIII GTNAC 3 cut(s) 173, 226, 267
MboII GAAGA 6 cut(s) 374, 396, 489, 541, 644, 692
MlsI TGGCCA 1 cut(s) 656
MluNI TGGCCA 1 cut(s) 656
Mly113I GGCGCC 1 cut(s) 255
MmeI TCCRAC 1 cut(s) 94
MnlI CCTC 8 cut(s) 94, 103, 136, 164, 268, 324, 445, 634
Mox20I TGGCCA 1 cut(s) 656
Mph1103I ATGCAT 1 cut(s) 206
MscI TGGCCA 1 cut(s) 656
MseI TTAA 1 cut(s) 179
MslI CAYNNNNRTG 2 cut(s) 363, 662
Msp20I TGGCCA 1 cut(s) 656
MspA1I CMGCKG 1 cut(s) 551
MspI CCGG 1 cut(s) 715
MvnI CGCG 1 cut(s) 215
MwoI GCNNNNNNNGC 3 cut(s) 210, 501, 683
NarI GGCGCC 1 cut(s) 255
NcoI CCATGG 1 cut(s) 723
NheI GCTAGC 1 cut(s) 397
NlaIII CATG 3 cut(s) 299, 362, 727
NlaIV GGNNCC 3 cut(s) 124, 145, 256
NmuCI GTSAC 2 cut(s) 173, 226
NsbI TGCGCA 1 cut(s) 462
NsiI ATGCAT 1 cut(s) 206
NspI RCATGY 1 cut(s) 299
NspV TTCGAA 1 cut(s) 197
PaeI GCATGC 1 cut(s) 299
PciSI GCTCTTC 1 cut(s) 502
PfeI GAWTC 2 cut(s) 220, 620
PkrI GCNGC 2 cut(s) 550, 553
PluTI GGCGCC 1 cut(s) 258
PspN4I GGNNCC 3 cut(s) 124, 145, 256
PstNI CAGNNNCTG 2 cut(s) 515, 551
PvuII CAGCTG 1 cut(s) 551
RsaI GTAC 4 cut(s) 161, 335, 357, 590
RsaNI GTAC 4 cut(s) 160, 334, 356, 589
RseI CAYNNNNRTG 2 cut(s) 363, 662
SapI GCTCTTC 1 cut(s) 502
SaqAI TTAA 1 cut(s) 179
SatI GCNGC 2 cut(s) 549, 552
SfaNI GCATC 3 cut(s) 14, 213, 502
SfoI GGCGCC 1 cut(s) 256
SfuI TTCGAA 1 cut(s) 197
SmiMI CAYNNNNRTG 2 cut(s) 363, 662
SmlI CTYRAG 1 cut(s) 87
SmoI CTYRAG 1 cut(s) 87
SpeI ACTAGT 1 cut(s) 416
SphI GCATGC 1 cut(s) 299
SsiI CCGC 2 cut(s) 215, 554
SspDI GGCGCC 1 cut(s) 254
SspMI CTAG 5 cut(s) 233, 398, 417, 585, 683
StyI CCWWGG 1 cut(s) 723
TaaI ACNGT 3 cut(s) 194, 355, 652
TaiI ACGT 1 cut(s) 396
TaqI TCGA 2 cut(s) 197, 207
TatI WGTACW 1 cut(s) 355
TfiI GAWTC 2 cut(s) 220, 620
Tru1I TTAA 1 cut(s) 179
Tru9I TTAA 1 cut(s) 179
TscAI CASTG 3 cut(s) 170, 470, 581
TseFI GTSAC 2 cut(s) 173, 226
TseI GCWGC 2 cut(s) 548, 551
Tsp45I GTSAC 2 cut(s) 173, 226
TspDTI ATGAA 1 cut(s) 375
TspGWI ACGGA 1 cut(s) 135
TspRI CASTG 3 cut(s) 170, 470, 581
XceI RCATGY 1 cut(s) 299
XspI CTAG 5 cut(s) 233, 398, 417, 585, 683
Zsp2I ATGCAT 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.