Rh1DG216900

Cysteine-rich repeat secretory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0010300
Physical Location & Seq
Forward (+)
42394320 .. 42396733
2414 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG216900.1

Sequence Viewer

Length: 825 bp
ATGAGCAACCTTCACTTACACTCTTCCATTTCCAAAACAATCTTAGCCCTGAGAATCTCTTCCCTTTTCTTTCTCTTTTCTTCACTCAATATCACCCATGGCTCCCCAATCAAAGCTAACATCTTCATCTATGCAGGCTGCTCACAAGAAAAGTACCAACCAAACTCACCCTTTGAAGCTAACCTCAACTCTTTCCTAACCTCAGTTGTTAGCTCATCCTCTCAAGCCTCTTACAACGCCTTTTCTATCGGAAACGGAACCTCAACATCACCGGAAGGTGCCATTTATGGCTTGTACCAGTGCAGAGGTGACTTAAGGATTTTTGACTGTTCGAAATGCATCGAAAGCGCGGTGAATCAAATCACTCTAGTCTGTCCCTACTCTTATGGCGCCTCTTTGCAGTTACAAGGTTGTTATGTTAGATACGAGCATGCTGAGTTTTTGGGCAAGCTTGATACAAGTTTGAGGTACAAGAAATGTAGTAAGAGTGTACATGAAGATGTTGAGTTCTTTCGGAGAAGAGATGACGTGCTAGCTGACTTGCAAGGGACTAGTGGATTTAGGGTTAGTAGTTCAGGCTTGGTTGAGGGTTTTGCGCAGTGTTTTGGGGATTTGAGCCAAAACGACTGCTCTTCTTGCGTCGCAGATGCCGTGGGAAAGCTGAAGAGTTTGTGTGGATCAGCAGCTGCGGTAGATGTGTTCTTGGCTCAGTGTTATGCTATGTACTGGGCTTCTGGCTACTATGATATCTCAGGTACGTACTGGTGTAATTCACAAATCCCTTCTTCGTTTATTTTTTTCATTTACTCAAGCCATCAATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stress-antifung

Protein Analysis

274

Amino Acids

30.08

Weight (kDa)

6.28

Isoelectric Point (pI)

41.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 47 - 144 1.6e-20 Salt stress response/antifungal
Stress-antifung PF01657 160 - 242 7.9e-10 Salt stress response/antifungal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010300)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G60720 AT3G60720 AT3G60720
fragaria_vesca FvH4_7g11310 FvH4_7g11310
malus_domestica MD02G1208100.v1.1 MD07G1117900.v1.1
prunus_persica Prupe.2G140900_v2.0.a1
pyrus_communis pycom02g17030 pycom07g10630
rosa_chinensis RchiOBHm_Chr1g0349511
rosa_laevigata RLG00000028545
rosa_multiflora Rmu_co8400581.1_g000001 Rmu_sc0002495.1_g000015
rosa_roxburghii Rroxscaffold_4G00305500
rosa_rugosa Rorug01G0204200
rosa_samantha Rh1AG221500 Rh1BG187600 Rh1CG206400 Rh1DG216900
rosa_wichuraiana Rw1G018830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 597
AccB1I GGYRCC 2 cut(s) 278, 389
AccII CGCG 1 cut(s) 350
AciI CCGC 2 cut(s) 350, 689
AclWI GGATC 1 cut(s) 685
AcuI CTGAAG 1 cut(s) 683
AcyI GRCGYC 1 cut(s) 390
AfaI GTAC 7 cut(s) 155, 296, 470, 492, 725, 757, 761
AflII CTTAAG 1 cut(s) 313
AgsI TTSAA 1 cut(s) 176
AhlI ACTAGT 1 cut(s) 551
AjiI CACGTC 1 cut(s) 529
AluBI AGCT 7 cut(s) 116, 179, 213, 451, 536, 661, 686
AluI AGCT 7 cut(s) 116, 179, 213, 451, 536, 661, 686
AlwI GGATC 1 cut(s) 685
AlwNI CAGNNNCTG 1 cut(s) 686
ApeKI GCWGC 3 cut(s) 138, 683, 686
Asp700I GAANNNNTTC 1 cut(s) 58
AspLEI GCGC 3 cut(s) 350, 392, 598
AsuHPI GGTGA 5 cut(s) 85, 159, 261, 320, 364
AsuII TTCGAA 1 cut(s) 332
AsuNHI GCTAGC 1 cut(s) 532
BanI GGYRCC 2 cut(s) 278, 389
BbvI GCAGC 3 cut(s) 125, 673, 695
BccI CCATC 1 cut(s) 822
BceAI ACGGC 1 cut(s) 635
BcuI ACTAGT 1 cut(s) 551
BfaI CTAG 3 cut(s) 368, 533, 552
BfoI RGCGCY 1 cut(s) 393
BfrI CTTAAG 1 cut(s) 313
BisI GCNGC 3 cut(s) 139, 684, 687
BlsI GCNGC 3 cut(s) 140, 685, 688
BmgBI CACGTC 1 cut(s) 529
BmiI GGNNCC 4 cut(s) 103, 259, 280, 391
BmrI ACTGGG 1 cut(s) 736
BmsI GCATC 2 cut(s) 348, 637
BmtI GCTAGC 1 cut(s) 536
BmuI ACTGGG 1 cut(s) 736
Bpu14I TTCGAA 1 cut(s) 332
BpuEI CTTGAG 2 cut(s) 207, 793
BsaAI YACGTR 1 cut(s) 759
BsaHI GRCGYC 1 cut(s) 390
BsaJI CCNNGG 2 cut(s) 97, 651
BsaWI WCCGGW 1 cut(s) 271
Bse1I ACTGG 3 cut(s) 298, 731, 767
BseDI CCNNGG 2 cut(s) 97, 651
BseGI GGATG 1 cut(s) 215
BseMII CTCAG 5 cut(s) 41, 216, 426, 722, 765
BseNI ACTGG 3 cut(s) 298, 731, 767
BseXI GCAGC 3 cut(s) 125, 673, 695
BsgI GTGCAG 1 cut(s) 322
Bsh1236I CGCG 1 cut(s) 350
BshNI GGYRCC 2 cut(s) 278, 389
BsiSI CCGG 1 cut(s) 272
BslFI GGGAC 2 cut(s) 360, 562
BsmFI GGGAC 2 cut(s) 360, 562
Bsp119I TTCGAA 1 cut(s) 332
Bsp1407I TGTACA 1 cut(s) 490
Bsp143I GATC 1 cut(s) 677
Bsp19I CCATGG 1 cut(s) 97
BspACI CCGC 2 cut(s) 350, 689
BspCNI CTCAG 5 cut(s) 42, 215, 427, 721, 764
BspFNI CGCG 1 cut(s) 350
BspLI GGNNCC 4 cut(s) 103, 259, 280, 391
BspOI GCTAGC 1 cut(s) 536
BspPI GGATC 1 cut(s) 685
BspQI GCTCTTC 1 cut(s) 637
BspT104I TTCGAA 1 cut(s) 332
BspT107I GGYRCC 2 cut(s) 278, 389
BspTI CTTAAG 1 cut(s) 313
BsrGI TGTACA 1 cut(s) 490
BsrI ACTGG 3 cut(s) 298, 731, 767
BssECI CCNNGG 2 cut(s) 97, 651
BssMI GATC 1 cut(s) 677
BssNI GRCGYC 1 cut(s) 390
BssT1I CCWWGG 1 cut(s) 97
Bst4CI ACNGT 1 cut(s) 329
Bst6I CTCTTC 5 cut(s) 28, 64, 514, 637, 659
BstACI GRCGYC 1 cut(s) 390
BstAFI CTTAAG 1 cut(s) 313
BstAUI TGTACA 1 cut(s) 490
BstBAI YACGTR 1 cut(s) 759
BstBI TTCGAA 1 cut(s) 332
BstC8I GCNNGC 4 cut(s) 136, 432, 449, 534
BstDEI CTNAG 6 cut(s) 43, 50, 202, 435, 708, 751
BstDSI CCRYGG 2 cut(s) 97, 651
BstF5I GGATG 1 cut(s) 215
BstFNI CGCG 1 cut(s) 350
BstH2I RGCGCY 1 cut(s) 393
BstHHI GCGC 3 cut(s) 350, 392, 598
BstKTI GATC 1 cut(s) 680
BstMBI GATC 1 cut(s) 677
BstMWI GCNNNNNNNGC 2 cut(s) 345, 636
BstNSI RCATGY 1 cut(s) 434
BstSNI TACGTA 1 cut(s) 759
BstUI CGCG 1 cut(s) 350
BstV1I GCAGC 3 cut(s) 125, 673, 695
BtgI CCRYGG 2 cut(s) 97, 651
BtrI CACGTC 1 cut(s) 529
BtsCI GGATG 1 cut(s) 215
BtsI GCAGTG 1 cut(s) 605
BtsIMutI CAGTG 3 cut(s) 305, 605, 716
Cac8I GCNNGC 4 cut(s) 136, 432, 449, 534
CaiI CAGNNNCTG 1 cut(s) 686
CfoI GCGC 3 cut(s) 350, 392, 598
CseI GACGC 1 cut(s) 628
Csp6I GTAC 7 cut(s) 154, 295, 469, 491, 724, 756, 760
CviAII CATG 3 cut(s) 98, 431, 494
CviQI GTAC 7 cut(s) 154, 295, 469, 491, 724, 756, 760
DdeI CTNAG 6 cut(s) 43, 50, 202, 435, 708, 751
DinI GGCGCC 1 cut(s) 391
DpnI GATC 1 cut(s) 679
DpnII GATC 1 cut(s) 677
Eam1104I CTCTTC 5 cut(s) 28, 64, 514, 637, 659
EarI CTCTTC 5 cut(s) 28, 64, 514, 637, 659
Eco105I TACGTA 1 cut(s) 759
Eco130I CCWWGG 1 cut(s) 97
Eco32I GATATC 1 cut(s) 748
Eco57I CTGAAG 1 cut(s) 683
EcoRV GATATC 1 cut(s) 748
EcoT14I CCWWGG 1 cut(s) 97
EcoT22I ATGCAT 1 cut(s) 341
EgeI GGCGCC 1 cut(s) 391
EheI GGCGCC 1 cut(s) 391
ErhI CCWWGG 1 cut(s) 97
FaeI CATG 3 cut(s) 101, 434, 497
FaqI GGGAC 2 cut(s) 360, 562
FatI CATG 3 cut(s) 97, 430, 493
Fnu4HI GCNGC 3 cut(s) 139, 684, 687
FokI GGATG 1 cut(s) 202
Fsp4HI GCNGC 3 cut(s) 139, 684, 687
FspBI CTAG 3 cut(s) 368, 533, 552
FspI TGCGCA 1 cut(s) 597
GlaI GCGC 3 cut(s) 349, 391, 597
GluI GCNGC 3 cut(s) 139, 684, 687
HaeII RGCGCY 1 cut(s) 393
HapII CCGG 1 cut(s) 272
HgaI GACGC 1 cut(s) 628
HhaI GCGC 3 cut(s) 350, 392, 598
Hin1I GRCGYC 1 cut(s) 390
Hin1II CATG 3 cut(s) 101, 434, 497
Hin6I GCGC 3 cut(s) 348, 390, 596
HinP1I GCGC 3 cut(s) 348, 390, 596
HindIII AAGCTT 1 cut(s) 449
HinfI GANTC 2 cut(s) 54, 355
HpaII CCGG 1 cut(s) 272
HphI GGTGA 5 cut(s) 85, 159, 261, 320, 364
Hpy166II GTNNAC 1 cut(s) 491
Hpy188I TCNGA 2 cut(s) 251, 516
Hpy8I GTNNAC 1 cut(s) 491
Hpy99I CGWCG 1 cut(s) 644
HpyAV CCTTC 3 cut(s) 20, 269, 792
HpyCH4III ACNGT 1 cut(s) 329
HpyCH4IV ACGT 2 cut(s) 528, 758
HpyCH4V TGCA 5 cut(s) 134, 303, 339, 400, 544
HpyF10VI GCNNNNNNNGC 2 cut(s) 345, 636
HpyF3I CTNAG 6 cut(s) 43, 50, 202, 435, 708, 751
HpySE526I ACGT 2 cut(s) 528, 758
Hsp92I GRCGYC 1 cut(s) 390
Hsp92II CATG 3 cut(s) 101, 434, 497
HspAI GCGC 3 cut(s) 348, 390, 596
KasI GGCGCC 1 cut(s) 389
Kzo9I GATC 1 cut(s) 677
LguI GCTCTTC 1 cut(s) 637
LmnI GCTCC 1 cut(s) 107
LpnPI CCDG 9 cut(s) 62, 120, 285, 311, 561, 712, 720, 738, 748
Lsp1109I GCAGC 3 cut(s) 125, 673, 695
LweI GCATC 2 cut(s) 348, 637
MaeI CTAG 3 cut(s) 368, 533, 552
MaeII ACGT 2 cut(s) 528, 758
MaeIII GTNAC 2 cut(s) 308, 402
MalI GATC 1 cut(s) 679
MboI GATC 1 cut(s) 677
MboII GAAGA 9 cut(s) 15, 51, 72, 115, 509, 531, 624, 676, 777
MluCI AATT 2 cut(s) 769, 818
Mly113I GGCGCC 1 cut(s) 390
MnlI CCTC 9 cut(s) 194, 211, 229, 238, 271, 299, 403, 459, 580
Mph1103I ATGCAT 1 cut(s) 341
MroXI GAANNNNTTC 1 cut(s) 58
MseI TTAA 1 cut(s) 314
MslI CAYNNNNRTG 1 cut(s) 498
MspA1I CMGCKG 1 cut(s) 686
MspCI CTTAAG 1 cut(s) 313
MspI CCGG 1 cut(s) 272
MvnI CGCG 1 cut(s) 350
MwoI GCNNNNNNNGC 2 cut(s) 345, 636
NarI GGCGCC 1 cut(s) 390
NcoI CCATGG 1 cut(s) 97
NdeII GATC 1 cut(s) 677
NheI GCTAGC 1 cut(s) 532
NlaIII CATG 3 cut(s) 101, 434, 497
NlaIV GGNNCC 4 cut(s) 103, 259, 280, 391
NmuCI GTSAC 1 cut(s) 308
NsbI TGCGCA 1 cut(s) 597
NsiI ATGCAT 1 cut(s) 341
NspI RCATGY 1 cut(s) 434
NspV TTCGAA 1 cut(s) 332
PaeI GCATGC 1 cut(s) 434
PciSI GCTCTTC 1 cut(s) 637
PcsI WCGNNNNNNNCGW 1 cut(s) 648
PdmI GAANNNNTTC 1 cut(s) 58
PfeI GAWTC 2 cut(s) 54, 355
PkrI GCNGC 3 cut(s) 140, 685, 688
PluTI GGCGCC 1 cut(s) 393
Ppu21I YACGTR 1 cut(s) 759
PspN4I GGNNCC 4 cut(s) 103, 259, 280, 391
PstNI CAGNNNCTG 1 cut(s) 686
PvuII CAGCTG 1 cut(s) 686
RsaI GTAC 7 cut(s) 155, 296, 470, 492, 725, 757, 761
RsaNI GTAC 7 cut(s) 154, 295, 469, 491, 724, 756, 760
RseI CAYNNNNRTG 1 cut(s) 498
SapI GCTCTTC 1 cut(s) 637
SaqAI TTAA 1 cut(s) 314
SatI GCNGC 3 cut(s) 139, 684, 687
Sau3AI GATC 1 cut(s) 677
SfaNI GCATC 2 cut(s) 348, 637
SfoI GGCGCC 1 cut(s) 391
SfuI TTCGAA 1 cut(s) 332
SmiMI CAYNNNNRTG 1 cut(s) 498
SmlI CTYRAG 3 cut(s) 222, 313, 808
SmoI CTYRAG 3 cut(s) 222, 313, 808
SnaBI TACGTA 1 cut(s) 759
SpeI ACTAGT 1 cut(s) 551
SphI GCATGC 1 cut(s) 434
Sse9I AATT 2 cut(s) 769, 818
SsiI CCGC 2 cut(s) 350, 689
SspDI GGCGCC 1 cut(s) 389
SspMI CTAG 3 cut(s) 368, 533, 552
StyI CCWWGG 1 cut(s) 97
TaaI ACNGT 1 cut(s) 329
TaiI ACGT 2 cut(s) 531, 761
TaqI TCGA 2 cut(s) 332, 342
TasI AATT 2 cut(s) 769, 818
TatI WGTACW 2 cut(s) 490, 723
TfiI GAWTC 2 cut(s) 54, 355
Tru1I TTAA 1 cut(s) 314
Tru9I TTAA 1 cut(s) 314
TscAI CASTG 3 cut(s) 305, 605, 716
TseFI GTSAC 1 cut(s) 308
TseI GCWGC 3 cut(s) 138, 683, 686
Tsp45I GTSAC 1 cut(s) 308
TspDTI ATGAA 3 cut(s) 115, 510, 790
TspGWI ACGGA 1 cut(s) 270
TspRI CASTG 3 cut(s) 305, 605, 716
Vha464I CTTAAG 1 cut(s) 313
XceI RCATGY 1 cut(s) 434
XmnI GAANNNNTTC 1 cut(s) 58
XspI CTAG 3 cut(s) 368, 533, 552
Zsp2I ATGCAT 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.