AT3G60820

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0010086
Physical Location & Seq
Reverse (-)
22471769 .. 22473971
2203 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G60820.1

Sequence Viewer

Length: 672 bp
ATGACTAAACAGCACGCGAACTGGTCTCCTTACGATAACAATGGAGGAACATGTGTGGCCATCGCTGGATCGGATTACTGTGTTATCGCCGCCGATACTCGGATGTCTACTGGTTACAGTATTCTTAGTCGCGATTACTCCAAAATCCATAAACTAGCGGACAGAGCTGTTTTGTCTTCCTCTGGCTTCCAGGCTGATGTGAAAGCTTTGCAGAAGGTTCTCAAATCCAGACACTTGATCTATCAACATCAGCATAACAAGCAGATGAGCTGTCCTGCAATGGCCCAGCTTCTCTCCAACACGCTTTATTTCAAGCGGTTTTTCCCCTACTATGCCTTTAATGTTCTAGGAGGGCTTGACGAGGAAGGAAAAGGGTGTGTCTTTACTTACGACGCTGTTGGCTCATACGAGAGAGTTGGATACGGTGCTCAAGGTTCTGGTTCCACACTCATCATGCCTTTCCTTGACAATCAGCTCAAGTCTCCAAGTCCTCTTTTGCTACCTAAACAGGATTCAAACACGCCCCTTTCCGAAGCTGAAGCAGTTGACTTGGTTAAAACTGTTTTCGCATCTGCCACAGAGAGGGATATCTACACTGGAGACAAGCTTGAGATTATGATACTTAAGGCCGACGGTATCAAGACCGAACTCATGGACCTGAGGAAAGACTAA

Protein Analysis

223

Amino Acids

24.64

Weight (kDa)

6.95

Isoelectric Point (pI)

40.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 13 - 207 5.1e-38 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010086)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G60820 AT3G60820 AT3G60820
fragaria_vesca FvH4_7g11920 FvH4_7g11920
malus_domestica MD02G1204000.v1.1 MD07G1122000.v1.1
prunus_persica Prupe.2G145800_v2.0.a1
pyrus_communis pycom02g16630 pycom07g11030
rosa_chinensis RchiOBHm_Chr1g0350601
rosa_laevigata RLG00000028469
rosa_multiflora Rmu_sc0014368.1_g000004 Rmu_sc0030263.1_g000001
rosa_roxburghii Rroxscaffold_4G00304680
rosa_rugosa Rorug01G0212500
rosa_samantha Rh1AG227000 Rh1BG195700 Rh1CG211800 Rh1DG223800
rosa_wichuraiana Rw1G019620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 107
AccII CGCG 2 cut(s) 17, 132
AciI CCGC 3 cut(s) 90, 158, 316
AclWI GGATC 1 cut(s) 76
AcoI YGGCCR 1 cut(s) 57
AcuI CTGAAG 1 cut(s) 558
AfiI CCNNNNNNNGG 2 cut(s) 99, 582
AflII CTTAAG 1 cut(s) 623
AflIII ACRYGT 1 cut(s) 50
AgsI TTSAA 2 cut(s) 313, 516
AjnI CCWGG 1 cut(s) 189
AluBI AGCT 7 cut(s) 167, 206, 270, 289, 475, 536, 607
AluI AGCT 7 cut(s) 167, 206, 270, 289, 475, 536, 607
Alw21I GWGCWC 1 cut(s) 430
Alw26I GTCTC 3 cut(s) 30, 486, 594
AlwI GGATC 1 cut(s) 76
AoxI GGCC 3 cut(s) 57, 282, 627
AspS9I GGNCC 2 cut(s) 283, 655
AvaII GGWCC 1 cut(s) 655
AxyI CCTNAGG 1 cut(s) 659
BalI TGGCCA 1 cut(s) 59
BbsI GAAGAC 1 cut(s) 168
Bbv12I GWGCWC 1 cut(s) 430
BccI CCATC 1 cut(s) 68
BciT130I CCWGG 1 cut(s) 191
BciVI GTATCC 1 cut(s) 413
BcoDI GTCTC 3 cut(s) 30, 486, 594
BfaI CTAG 2 cut(s) 155, 347
BfrI CTTAAG 1 cut(s) 623
BfuI GTATCC 1 cut(s) 413
BisI GCNGC 1 cut(s) 90
BlsI GCNGC 1 cut(s) 91
Bme1390I CCNGG 1 cut(s) 191
Bme18I GGWCC 1 cut(s) 655
BmgT120I GGNCC 2 cut(s) 283, 655
BmiI GGNNCC 1 cut(s) 442
BmrFI CCNGG 1 cut(s) 191
BmsI GCATC 1 cut(s) 578
BpiI GAAGAC 1 cut(s) 168
BpmI CTGGAG 1 cut(s) 618
BpuEI CTTGAG 3 cut(s) 414, 461, 629
BsaI GGTCTC 1 cut(s) 30
Bsc4I CCNNNNNNNGG 2 cut(s) 99, 582
Bse1I ACTGG 3 cut(s) 26, 115, 601
Bse21I CCTNAGG 1 cut(s) 659
Bse3DI GCAATG 1 cut(s) 285
BseBI CCWGG 1 cut(s) 191
BseGI GGATG 1 cut(s) 108
BseLI CCNNNNNNNGG 2 cut(s) 99, 582
BseMI GCAATG 1 cut(s) 285
BseMII CTCAG 1 cut(s) 650
BseNI ACTGG 3 cut(s) 26, 115, 601
BseYI CCCAGC 1 cut(s) 285
Bsh1236I CGCG 2 cut(s) 17, 132
BshFI GGCC 3 cut(s) 59, 284, 629
BsiHKAI GWGCWC 1 cut(s) 430
BslI CCNNNNNNNGG 2 cut(s) 99, 582
BsmAI GTCTC 3 cut(s) 30, 486, 594
BsnI GGCC 3 cut(s) 59, 284, 629
Bso31I GGTCTC 1 cut(s) 30
Bsp1286I GDGCHC 1 cut(s) 430
Bsp143I GATC 2 cut(s) 68, 237
Bsp68I TCGCGA 1 cut(s) 132
BspACI CCGC 3 cut(s) 90, 158, 316
BspANI GGCC 3 cut(s) 59, 284, 629
BspCNI CTCAG 1 cut(s) 651
BspFNI CGCG 2 cut(s) 17, 132
BspLI GGNNCC 1 cut(s) 442
BspPI GGATC 1 cut(s) 76
BspTI CTTAAG 1 cut(s) 623
BspTNI GGTCTC 1 cut(s) 30
BsrDI GCAATG 1 cut(s) 285
BsrI ACTGG 3 cut(s) 26, 115, 601
BssMI GATC 2 cut(s) 68, 237
Bst2UI CCWGG 1 cut(s) 191
Bst4CI ACNGT 5 cut(s) 80, 119, 425, 562, 635
BstAFI CTTAAG 1 cut(s) 623
BstC8I GCNNGC 1 cut(s) 15
BstDEI CTNAG 2 cut(s) 125, 659
BstF5I GGATG 1 cut(s) 108
BstFNI CGCG 2 cut(s) 17, 132
BstKTI GATC 2 cut(s) 71, 240
BstMAI GTCTC 3 cut(s) 30, 486, 594
BstMBI GATC 2 cut(s) 68, 237
BstMWI GCNNNNNNNGC 2 cut(s) 164, 259
BstNI CCWGG 1 cut(s) 191
BstNSI RCATGY 1 cut(s) 54
BstSCI CCNGG 1 cut(s) 189
BstUI CGCG 2 cut(s) 17, 132
BstV2I GAAGAC 1 cut(s) 168
Bsu36I CCTNAGG 1 cut(s) 659
BsuI GTATCC 1 cut(s) 413
BsuRI GGCC 3 cut(s) 59, 284, 629
BtgZI GCGATG 1 cut(s) 46
BtsCI GGATG 1 cut(s) 108
BtsIMutI CAGTG 1 cut(s) 594
BtuMI TCGCGA 1 cut(s) 132
Cac8I GCNNGC 1 cut(s) 15
Cfr13I GGNCC 2 cut(s) 283, 655
CseI GACGC 1 cut(s) 401
CviAII CATG 3 cut(s) 51, 454, 652
DdeI CTNAG 2 cut(s) 125, 659
DpnI GATC 2 cut(s) 70, 239
DpnII GATC 2 cut(s) 68, 237
EaeI YGGCCR 1 cut(s) 57
Eco31I GGTCTC 1 cut(s) 30
Eco32I GATATC 1 cut(s) 589
Eco47I GGWCC 1 cut(s) 655
Eco57I CTGAAG 1 cut(s) 558
Eco81I CCTNAGG 1 cut(s) 659
EcoRII CCWGG 1 cut(s) 189
EcoRV GATATC 1 cut(s) 589
FaeI CATG 3 cut(s) 54, 457, 655
FaiI YATR 8 cut(s) 52, 150, 255, 333, 406, 455, 617, 653
FatI CATG 3 cut(s) 50, 453, 651
FblI GTMKAC 1 cut(s) 107
Fnu4HI GCNGC 1 cut(s) 90
FokI GGATG 1 cut(s) 115
Fsp4HI GCNGC 1 cut(s) 90
FspBI CTAG 2 cut(s) 155, 347
GluI GCNGC 1 cut(s) 90
GsaI CCCAGC 1 cut(s) 289
GsuI CTGGAG 1 cut(s) 618
HaeIII GGCC 3 cut(s) 59, 284, 629
HgaI GACGC 1 cut(s) 401
Hin1II CATG 3 cut(s) 54, 457, 655
HincII GTYRAC 1 cut(s) 547
HindII GTYRAC 1 cut(s) 547
HindIII AAGCTT 2 cut(s) 204, 605
HinfI GANTC 1 cut(s) 512
Hpy166II GTNNAC 2 cut(s) 108, 547
Hpy188I TCNGA 3 cut(s) 73, 102, 532
Hpy188III TCNNGA 3 cut(s) 131, 228, 640
Hpy8I GTNNAC 2 cut(s) 108, 547
Hpy99I CGWCG 2 cut(s) 395, 635
HpyAV CCTTC 2 cut(s) 208, 359
HpyCH4III ACNGT 5 cut(s) 80, 119, 425, 562, 635
HpyCH4V TGCA 2 cut(s) 211, 278
HpyF10VI GCNNNNNNNGC 2 cut(s) 164, 259
HpyF3I CTNAG 2 cut(s) 125, 659
Hsp92II CATG 3 cut(s) 54, 457, 655
Kzo9I GATC 2 cut(s) 68, 237
LweI GCATC 1 cut(s) 578
MaeI CTAG 2 cut(s) 155, 347
MaeIII GTNAC 1 cut(s) 113
MalI GATC 2 cut(s) 70, 239
MboI GATC 2 cut(s) 68, 237
MboII GAAGA 1 cut(s) 168
MhlI GDGCHC 1 cut(s) 430
MlsI TGGCCA 1 cut(s) 59
MluNI TGGCCA 1 cut(s) 59
MmeI TCCRAC 2 cut(s) 321, 397
MnlI CCTC 7 cut(s) 38, 190, 344, 355, 501, 576, 654
Mox20I TGGCCA 1 cut(s) 59
MscI TGGCCA 1 cut(s) 59
MseI TTAA 3 cut(s) 339, 555, 624
Msp20I TGGCCA 1 cut(s) 59
MspCI CTTAAG 1 cut(s) 623
MspR9I CCNGG 1 cut(s) 191
MvaI CCWGG 1 cut(s) 191
MvnI CGCG 2 cut(s) 17, 132
MwoI GCNNNNNNNGC 2 cut(s) 164, 259
NdeII GATC 2 cut(s) 68, 237
NlaIII CATG 3 cut(s) 54, 457, 655
NlaIV GGNNCC 1 cut(s) 442
NruI TCGCGA 1 cut(s) 132
NspI RCATGY 1 cut(s) 54
PciI ACATGT 1 cut(s) 50
PfeI GAWTC 1 cut(s) 512
PkrI GCNGC 1 cut(s) 91
PscI ACATGT 1 cut(s) 50
Psp6I CCWGG 1 cut(s) 189
PspFI CCCAGC 1 cut(s) 285
PspGI CCWGG 1 cut(s) 189
PspN4I GGNNCC 1 cut(s) 442
PspPI GGNCC 2 cut(s) 283, 655
RruI TCGCGA 1 cut(s) 132
SaqAI TTAA 3 cut(s) 339, 555, 624
SatI GCNGC 1 cut(s) 90
Sau3AI GATC 2 cut(s) 68, 237
Sau96I GGNCC 2 cut(s) 283, 655
ScrFI CCNGG 1 cut(s) 191
SduI GDGCHC 1 cut(s) 430
SfaNI GCATC 1 cut(s) 578
SinI GGWCC 1 cut(s) 655
SmlI CTYRAG 4 cut(s) 429, 476, 608, 623
SmoI CTYRAG 4 cut(s) 429, 476, 608, 623
SsiI CCGC 3 cut(s) 90, 158, 316
SspMI CTAG 2 cut(s) 155, 347
StyD4I CCNGG 1 cut(s) 189
TaaI ACNGT 5 cut(s) 80, 119, 425, 562, 635
TaqII GACCGA 1 cut(s) 659
TauI GCSGC 1 cut(s) 92
TfiI GAWTC 1 cut(s) 512
Tru1I TTAA 3 cut(s) 339, 555, 624
Tru9I TTAA 3 cut(s) 339, 555, 624
TscAI CASTG 1 cut(s) 601
TspRI CASTG 1 cut(s) 601
Vha464I CTTAAG 1 cut(s) 623
VpaK11BI GGWCC 1 cut(s) 655
XceI RCATGY 1 cut(s) 54
XmiI GTMKAC 1 cut(s) 107
XspI CTAG 2 cut(s) 155, 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.