Rh1CG211800

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0010086
Physical Location & Seq
Reverse (-)
45662133 .. 45664714
2582 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG211800.1

Sequence Viewer

Length: 774 bp
ATGACCAAGCAGCACGCTAACTGGTCCCCCTACGACAACAATGGCGGGTACTCTCCTTTTCCATCTACTCTAAGCTCGTCTGCGCTTTTCAGCGATTGGAAATTTTTTTTTTTTTTTGGTGATTTTTATGGATTGGGCGATTTTTGCAGATCCGTGGTGGCGATTGCCGGAGCTGATTACTGTGTGATTGCCGCCGATACTCGGATGTCGACGGGTTACAGTATTCTCACCCGCGGCTACTCCAAAATCAGCAAATTAGCGGACAAAGCTTTTATGGCCTCTTCTGGTTTTCAAGCTGATGTGAAAGCTTTACAGAAGCATTTGACAGCTAAGCACTTGATTTATCAGCATCAGCATAACAAACAAATGAGCTGCCCTGCAATGGCTCAACTACTTTCCAATACTCTTTACTACAAACGCTTCTTTCCCTATTACGCCTTTAATGTTTTGGGTGGTCTAGACACTGAAGGGAAGGGTTGTGTCTTCACATATGATGCTGTTGGTTCTTATGAGAGGGTTGGATATAGTGCCCAAGGTTCTGGTTCCACACTCATCATGCCTTTCTTGGATAACCAACTGAAGTCTCCCAGTCCTCTCTTGTTGCCTGCTGTGGATGCTGTAACACCACTTTCAGAAGGAGAAGCAATTGATTTGGTCAAAACTGTTTTTGCATCTGCCACTGAGAGGGATATCTACACTGGAGACAGGCTTGAAATTTATGTCCTAAATGCTCGCGGCACTCATCATGAATACATGGAACTGAGGAAAGATTGA

Protein Analysis

257

Amino Acids

28.54

Weight (kDa)

6.65

Isoelectric Point (pI)

39.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 51 - 241 3e-34 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010086)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G60820 AT3G60820 AT3G60820
fragaria_vesca FvH4_7g11920 FvH4_7g11920
malus_domestica MD02G1204000.v1.1 MD07G1122000.v1.1
prunus_persica Prupe.2G145800_v2.0.a1
pyrus_communis pycom02g16630 pycom07g11030
rosa_chinensis RchiOBHm_Chr1g0350601
rosa_laevigata RLG00000028469
rosa_multiflora Rmu_sc0014368.1_g000004 Rmu_sc0030263.1_g000001
rosa_roxburghii Rroxscaffold_4G00304680
rosa_rugosa Rorug01G0212500
rosa_samantha Rh1AG227000 Rh1BG195700 Rh1CG211800 Rh1DG223800
rosa_wichuraiana Rw1G019620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 209
AccII CGCG 2 cut(s) 234, 735
AciI CCGC 6 cut(s) 45, 192, 232, 234, 260, 735
AclWI GGATC 1 cut(s) 144
AcsI RAATTY 2 cut(s) 101, 714
AcuI CTGAAG 2 cut(s) 486, 599
AfaI GTAC 1 cut(s) 50
AfiI CCNNNNNNNGG 3 cut(s) 201, 382, 684
AgsI TTSAA 2 cut(s) 293, 713
AluBI AGCT 7 cut(s) 75, 173, 269, 296, 308, 329, 372
AluI AGCT 7 cut(s) 75, 173, 269, 296, 308, 329, 372
Alw26I GTCTC 2 cut(s) 588, 696
AlwI GGATC 1 cut(s) 144
AoxI GGCC 1 cut(s) 276
ApeKI GCWGC 2 cut(s) 10, 372
ApoI RAATTY 2 cut(s) 101, 714
AspLEI GCGC 1 cut(s) 85
AspS9I GGNCC 1 cut(s) 24
AsuHPI GGTGA 2 cut(s) 131, 220
AvaII GGWCC 1 cut(s) 24
BaeGI GKGCMC 1 cut(s) 532
BbsI GAAGAC 1 cut(s) 475
BbvI GCAGC 2 cut(s) 22, 359
BccI CCATC 1 cut(s) 70
BcoDI GTCTC 2 cut(s) 588, 696
BfaI CTAG 1 cut(s) 458
BisI GCNGC 5 cut(s) 11, 192, 235, 373, 736
BlpI GCTNAGC 1 cut(s) 330
BlsI GCNGC 5 cut(s) 12, 193, 236, 374, 737
Bme18I GGWCC 1 cut(s) 24
BmgT120I GGNCC 1 cut(s) 24
BmiI GGNNCC 2 cut(s) 26, 544
BmrI ACTGGG 1 cut(s) 582
BmsI GCATC 4 cut(s) 358, 484, 604, 680
BmuI ACTGGG 1 cut(s) 582
BpiI GAAGAC 1 cut(s) 475
BpmI CTGGAG 1 cut(s) 720
Bpu1102I GCTNAGC 1 cut(s) 330
BsaJI CCNNGG 3 cut(s) 153, 232, 532
Bsc4I CCNNNNNNNGG 3 cut(s) 201, 382, 684
Bse1I ACTGG 3 cut(s) 26, 588, 703
Bse3DI GCAATG 1 cut(s) 387
BseDI CCNNGG 3 cut(s) 153, 232, 532
BseGI GGATG 2 cut(s) 210, 619
BseLI CCNNNNNNNGG 3 cut(s) 201, 382, 684
BseMI GCAATG 1 cut(s) 387
BseMII CTCAG 2 cut(s) 672, 752
BseNI ACTGG 3 cut(s) 26, 588, 703
BseSI GKGCMC 1 cut(s) 532
BseXI GCAGC 2 cut(s) 22, 359
Bsh1236I CGCG 2 cut(s) 234, 735
BshFI GGCC 1 cut(s) 278
BsiSI CCGG 1 cut(s) 168
BslFI GGGAC 1 cut(s) 10
BslI CCNNNNNNNGG 3 cut(s) 201, 382, 684
BsmAI GTCTC 2 cut(s) 588, 696
BsmFI GGGAC 1 cut(s) 10
BsnI GGCC 1 cut(s) 278
Bsp1286I GDGCHC 1 cut(s) 532
Bsp143I GATC 1 cut(s) 149
Bsp1720I GCTNAGC 1 cut(s) 330
BspACI CCGC 6 cut(s) 45, 192, 232, 234, 260, 735
BspANI GGCC 1 cut(s) 278
BspCNI CTCAG 2 cut(s) 673, 753
BspFNI CGCG 2 cut(s) 234, 735
BspHI TCATGA 1 cut(s) 745
BspLI GGNNCC 2 cut(s) 26, 544
BspPI GGATC 1 cut(s) 144
BsrDI GCAATG 1 cut(s) 387
BsrI ACTGG 3 cut(s) 26, 588, 703
BssECI CCNNGG 3 cut(s) 153, 232, 532
BssMI GATC 1 cut(s) 149
BssT1I CCWWGG 1 cut(s) 532
Bst4CI ACNGT 3 cut(s) 182, 221, 664
Bst6I CTCTTC 1 cut(s) 286
BstC8I GCNNGC 3 cut(s) 15, 606, 733
BstDEI CTNAG 4 cut(s) 71, 330, 681, 761
BstDSI CCRYGG 2 cut(s) 153, 232
BstF5I GGATG 2 cut(s) 210, 619
BstFNI CGCG 2 cut(s) 234, 735
BstHHI GCGC 1 cut(s) 85
BstKTI GATC 1 cut(s) 152
BstMAI GTCTC 2 cut(s) 588, 696
BstMBI GATC 1 cut(s) 149
BstMWI GCNNNNNNNGC 4 cut(s) 144, 266, 275, 614
BstSLI GKGCMC 1 cut(s) 532
BstUI CGCG 2 cut(s) 234, 735
BstV1I GCAGC 2 cut(s) 22, 359
BstV2I GAAGAC 1 cut(s) 475
BstX2I RGATCY 1 cut(s) 149
BstXI CCANNNNNNTGG 1 cut(s) 539
BstYI RGATCY 1 cut(s) 149
BsuRI GGCC 1 cut(s) 278
BtgI CCRYGG 2 cut(s) 153, 232
BtsCI GGATG 2 cut(s) 210, 619
BtsIMutI CAGTG 3 cut(s) 462, 678, 696
Cac8I GCNNGC 3 cut(s) 15, 606, 733
CciI TCATGA 1 cut(s) 745
CfoI GCGC 1 cut(s) 85
Cfr13I GGNCC 1 cut(s) 24
Cfr42I CCGCGG 1 cut(s) 235
Csp6I GTAC 1 cut(s) 49
CviAII CATG 3 cut(s) 556, 746, 754
CviQI GTAC 1 cut(s) 49
DdeI CTNAG 4 cut(s) 71, 330, 681, 761
DpnI GATC 1 cut(s) 151
DpnII GATC 1 cut(s) 149
Eam1104I CTCTTC 1 cut(s) 286
EarI CTCTTC 1 cut(s) 286
Eco130I CCWWGG 1 cut(s) 532
Eco32I GATATC 1 cut(s) 691
Eco47I GGWCC 1 cut(s) 24
Eco57I CTGAAG 2 cut(s) 486, 599
EcoRV GATATC 1 cut(s) 691
EcoT14I CCWWGG 1 cut(s) 532
ErhI CCWWGG 1 cut(s) 532
FaeI CATG 3 cut(s) 559, 749, 757
FaqI GGGAC 1 cut(s) 10
FatI CATG 3 cut(s) 555, 745, 753
FauI CCCGC 2 cut(s) 38, 239
FauNDI CATATG 1 cut(s) 490
FblI GTMKAC 1 cut(s) 209
Fnu4HI GCNGC 5 cut(s) 11, 192, 235, 373, 736
FokI GGATG 2 cut(s) 217, 626
Fsp4HI GCNGC 5 cut(s) 11, 192, 235, 373, 736
FspBI CTAG 1 cut(s) 458
GlaI GCGC 1 cut(s) 84
GluI GCNGC 5 cut(s) 11, 192, 235, 373, 736
GsuI CTGGAG 1 cut(s) 720
HaeIII GGCC 1 cut(s) 278
HapII CCGG 1 cut(s) 168
HhaI GCGC 1 cut(s) 85
Hin1II CATG 3 cut(s) 559, 749, 757
Hin6I GCGC 1 cut(s) 83
HinP1I GCGC 1 cut(s) 83
HincII GTYRAC 1 cut(s) 210
HindII GTYRAC 1 cut(s) 210
HindIII AAGCTT 2 cut(s) 267, 306
HpaII CCGG 1 cut(s) 168
HphI GGTGA 2 cut(s) 131, 220
Hpy166II GTNNAC 1 cut(s) 210
Hpy188I TCNGA 2 cut(s) 204, 634
Hpy188III TCNNGA 2 cut(s) 458, 746
Hpy8I GTNNAC 1 cut(s) 210
Hpy99I CGWCG 1 cut(s) 214
HpyAV CCTTC 3 cut(s) 461, 466, 629
HpyCH4III ACNGT 3 cut(s) 182, 221, 664
HpyCH4V TGCA 3 cut(s) 147, 380, 671
HpyF10VI GCNNNNNNNGC 4 cut(s) 144, 266, 275, 614
HpyF3I CTNAG 4 cut(s) 71, 330, 681, 761
Hsp92II CATG 3 cut(s) 559, 749, 757
HspAI GCGC 1 cut(s) 83
KspI CCGCGG 1 cut(s) 235
Kzo9I GATC 1 cut(s) 149
LmnI GCTCC 1 cut(s) 170
LpnPI CCDG 9 cut(s) 7, 181, 270, 390, 525, 601, 618, 684, 691
Lsp1109I GCAGC 2 cut(s) 22, 359
LweI GCATC 4 cut(s) 358, 484, 604, 680
MaeI CTAG 1 cut(s) 458
MaeIII GTNAC 2 cut(s) 215, 619
MalI GATC 1 cut(s) 151
MboI GATC 1 cut(s) 149
MboII GAAGA 2 cut(s) 273, 475
MfeI CAATTG 1 cut(s) 645
MflI RGATCY 1 cut(s) 149
MhlI GDGCHC 1 cut(s) 532
MluCI AATT 4 cut(s) 101, 254, 645, 714
MmeI TCCRAC 1 cut(s) 499
MnlI CCTC 5 cut(s) 289, 507, 603, 678, 756
MseI TTAA 1 cut(s) 441
MspA1I CMGCKG 1 cut(s) 234
MspI CCGG 1 cut(s) 168
MunI CAATTG 1 cut(s) 645
MvnI CGCG 2 cut(s) 234, 735
MwoI GCNNNNNNNGC 4 cut(s) 144, 266, 275, 614
NdeI CATATG 1 cut(s) 490
NdeII GATC 1 cut(s) 149
NlaIII CATG 3 cut(s) 559, 749, 757
NlaIV GGNNCC 2 cut(s) 26, 544
PagI TCATGA 1 cut(s) 745
PkrI GCNGC 5 cut(s) 12, 193, 236, 374, 737
PspN4I GGNNCC 2 cut(s) 26, 544
PspPI GGNCC 1 cut(s) 24
PsuI RGATCY 1 cut(s) 149
RsaI GTAC 1 cut(s) 50
RsaNI GTAC 1 cut(s) 49
SacII CCGCGG 1 cut(s) 235
SalI GTCGAC 1 cut(s) 208
SaqAI TTAA 1 cut(s) 441
SatI GCNGC 5 cut(s) 11, 192, 235, 373, 736
Sau3AI GATC 1 cut(s) 149
Sau96I GGNCC 1 cut(s) 24
SduI GDGCHC 1 cut(s) 532
SetI ASST 8 cut(s) 77, 175, 271, 298, 310, 331, 374, 538
SfaNI GCATC 4 cut(s) 358, 484, 604, 680
Sfr303I CCGCGG 1 cut(s) 235
SgrBI CCGCGG 1 cut(s) 235
SinI GGWCC 1 cut(s) 24
Sse9I AATT 4 cut(s) 101, 254, 645, 714
SsiI CCGC 6 cut(s) 45, 192, 232, 234, 260, 735
SspMI CTAG 1 cut(s) 458
StyI CCWWGG 1 cut(s) 532
TaaI ACNGT 3 cut(s) 182, 221, 664
TaqI TCGA 1 cut(s) 209
TasI AATT 4 cut(s) 101, 254, 645, 714
TauI GCSGC 3 cut(s) 194, 237, 738
Tru1I TTAA 1 cut(s) 441
Tru9I TTAA 1 cut(s) 441
TscAI CASTG 3 cut(s) 469, 685, 703
TseI GCWGC 2 cut(s) 10, 372
TspDTI ATGAA 1 cut(s) 762
TspGWI ACGGA 1 cut(s) 142
TspRI CASTG 3 cut(s) 469, 685, 703
VpaK11BI GGWCC 1 cut(s) 24
XapI RAATTY 2 cut(s) 101, 714
XbaI TCTAGA 1 cut(s) 457
XmiI GTMKAC 1 cut(s) 209
XspI CTAG 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.