MD02G1204000.v1.1

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0010086
Physical Location & Seq
Forward (+)
20381285 .. 20384380
3096 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1204000.v1.1.491

Sequence Viewer

Length: 672 bp
ATGACCAAGCAGCACGCTAACTGGTCCCCCTATGACAACAATGGCGGGTCCTGTGTGGCGATCGCCGGAGCCAACTACTGTGTGATTGCCGCCGATACTCGGATGTCGACCGGTTACAGTATTCTCACCCGCGACTACTCCAAAATCTGCAAATTAGCGGACAAAGCTGTATTGGCATCTTCTGGTTTTCAAGCTGATGTGAAAGCTTTGCAAAAGCATTTGGCAGCTAAGCACTTGACTTATCAGCATCAACATAACAAACAAATGAGCTGCCCTGCGATGGCTCAACTGCTTTCCAACACCCTTTACTACAAGCGGTTCTTCCCCTACTACGCATTTAATGTTTTGGGTGGCTTAGATAGTGAAGGTAAGGGTTGCGTCTTCACATATGATGCTGTTGGTTCCTATGAGAGGGTTGGGTATAGCTCCCAAGGTTCTGGTTCTACACTTATCATTCCTTTTCTGGACAACCAATTGAAGTCTCCTAGCCCACTCCTATTGCCTGCCCAGGATGCTGTCACACCGCTGTCGGAAGCAGAAGCGATCGACTTGGTCAAAACTTGTTTTGCATCTGCAACTGAAAGAGATATTTACACTGGCGACAGGCTGGAGATTGTTGTCCTCAATGCGGATGGCACCAGATATGAGTATATGGAGCTGAGGAAGGACTGA

Protein Analysis

224

Amino Acids

24.52

Weight (kDa)

6.51

Isoelectric Point (pI)

39.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 13 - 207 1.4e-36 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010086)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G60820 AT3G60820 AT3G60820
fragaria_vesca FvH4_7g11920 FvH4_7g11920
malus_domestica MD02G1204000.v1.1 MD07G1122000.v1.1
prunus_persica Prupe.2G145800_v2.0.a1
pyrus_communis pycom02g16630 pycom07g11030
rosa_chinensis RchiOBHm_Chr1g0350601
rosa_laevigata RLG00000028469
rosa_multiflora Rmu_sc0014368.1_g000004 Rmu_sc0030263.1_g000001
rosa_roxburghii Rroxscaffold_4G00304680
rosa_rugosa Rorug01G0212500
rosa_samantha Rh1AG227000 Rh1BG195700 Rh1CG211800 Rh1DG223800
rosa_wichuraiana Rw1G019620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 635
AccI GTMKAC 1 cut(s) 107
AccII CGCG 1 cut(s) 132
AciI CCGC 7 cut(s) 45, 90, 130, 158, 316, 524, 629
AfiI CCNNNNNNNGG 4 cut(s) 99, 280, 411, 628
AgeI ACCGGT 1 cut(s) 110
AgsI TTSAA 2 cut(s) 191, 478
AjnI CCWGG 1 cut(s) 507
AluBI AGCT 7 cut(s) 167, 194, 206, 227, 270, 426, 658
AluI AGCT 7 cut(s) 167, 194, 206, 227, 270, 426, 658
Alw26I GTCTC 1 cut(s) 486
ApeKI GCWGC 3 cut(s) 10, 224, 270
AsiGI ACCGGT 1 cut(s) 110
AsiSI GCGATCGC 1 cut(s) 63
AspS9I GGNCC 2 cut(s) 24, 48
AsuHPI GGTGA 1 cut(s) 118
AvaII GGWCC 2 cut(s) 24, 48
BanI GGYRCC 1 cut(s) 635
BbsI GAAGAC 1 cut(s) 373
BbvCI CCTCAGC 1 cut(s) 659
BbvI GCAGC 3 cut(s) 22, 236, 257
BccI CCATC 2 cut(s) 274, 626
BciT130I CCWGG 1 cut(s) 509
BcoDI GTCTC 1 cut(s) 486
BfaI CTAG 1 cut(s) 486
BisI GCNGC 4 cut(s) 11, 90, 225, 271
BlpI GCTNAGC 1 cut(s) 228
BlsI GCNGC 4 cut(s) 12, 91, 226, 272
Bme1390I CCNGG 1 cut(s) 509
Bme18I GGWCC 2 cut(s) 24, 48
BmgT120I GGNCC 2 cut(s) 24, 48
BmiI GGNNCC 5 cut(s) 26, 49, 70, 403, 637
BmrFI CCNGG 1 cut(s) 509
BmsI GCATC 5 cut(s) 185, 256, 382, 502, 578
BpiI GAAGAC 1 cut(s) 373
BpmI CTGGAG 1 cut(s) 629
Bpu10I CCTNAGC 1 cut(s) 659
Bpu1102I GCTNAGC 1 cut(s) 228
BsaJI CCNNGG 2 cut(s) 430, 507
BsaWI WCCGGW 1 cut(s) 110
Bsc4I CCNNNNNNNGG 4 cut(s) 99, 280, 411, 628
Bse118I RCCGGY 1 cut(s) 110
Bse1I ACTGG 2 cut(s) 26, 601
BseBI CCWGG 1 cut(s) 509
BseDI CCNNGG 2 cut(s) 430, 507
BseGI GGATG 3 cut(s) 108, 517, 637
BseLI CCNNNNNNNGG 4 cut(s) 99, 280, 411, 628
BseMII CTCAG 1 cut(s) 650
BseNI ACTGG 2 cut(s) 26, 601
BseXI GCAGC 3 cut(s) 22, 236, 257
Bsh1236I CGCG 1 cut(s) 132
Bsh1285I CGRYCG 3 cut(s) 63, 111, 546
BshNI GGYRCC 1 cut(s) 635
BshTI ACCGGT 1 cut(s) 110
BsiEI CGRYCG 3 cut(s) 63, 111, 546
BsiSI CCGG 2 cut(s) 66, 111
BslFI GGGAC 1 cut(s) 10
BslI CCNNNNNNNGG 4 cut(s) 99, 280, 411, 628
BsmAI GTCTC 1 cut(s) 486
BsmFI GGGAC 1 cut(s) 10
Bsp143I GATC 2 cut(s) 60, 543
Bsp1720I GCTNAGC 1 cut(s) 228
BspACI CCGC 7 cut(s) 45, 90, 130, 158, 316, 524, 629
BspCNI CTCAG 1 cut(s) 651
BspFNI CGCG 1 cut(s) 132
BspLI GGNNCC 5 cut(s) 26, 49, 70, 403, 637
BspT107I GGYRCC 1 cut(s) 635
BsrFI RCCGGY 1 cut(s) 110
BsrI ACTGG 2 cut(s) 26, 601
BssAI RCCGGY 1 cut(s) 110
BssECI CCNNGG 2 cut(s) 430, 507
BssMI GATC 2 cut(s) 60, 543
BssT1I CCWWGG 1 cut(s) 430
Bst2UI CCWGG 1 cut(s) 509
Bst4CI ACNGT 2 cut(s) 80, 119
BstC8I GCNNGC 2 cut(s) 15, 504
BstDEI CTNAG 3 cut(s) 228, 355, 659
BstENI CCTNNNNNAGG 1 cut(s) 409
BstF5I GGATG 3 cut(s) 108, 517, 637
BstFNI CGCG 1 cut(s) 132
BstKTI GATC 2 cut(s) 63, 546
BstMAI GTCTC 1 cut(s) 486
BstMBI GATC 2 cut(s) 60, 543
BstMCI CGRYCG 3 cut(s) 63, 111, 546
BstMWI GCNNNNNNNGC 3 cut(s) 164, 173, 512
BstNI CCWGG 1 cut(s) 509
BstSCI CCNGG 1 cut(s) 507
BstUI CGCG 1 cut(s) 132
BstV1I GCAGC 3 cut(s) 22, 236, 257
BstV2I GAAGAC 1 cut(s) 373
BstXI CCANNNNNNTGG 1 cut(s) 437
BtgZI GCGATG 1 cut(s) 293
BtsCI GGATG 3 cut(s) 108, 517, 637
BtsIMutI CAGTG 1 cut(s) 594
Cac8I GCNNGC 2 cut(s) 15, 504
Cfr10I RCCGGY 1 cut(s) 110
Cfr13I GGNCC 2 cut(s) 24, 48
CseI GACGC 1 cut(s) 367
CspAI ACCGGT 1 cut(s) 110
CspCI CAANNNNNGTGG 2 cut(s) 480, 515
DdeI CTNAG 3 cut(s) 228, 355, 659
DpnI GATC 2 cut(s) 62, 545
DpnII GATC 2 cut(s) 60, 543
Eco130I CCWWGG 1 cut(s) 430
Eco47I GGWCC 2 cut(s) 24, 48
EcoNI CCTNNNNNAGG 1 cut(s) 409
EcoO109I RGGNCCY 1 cut(s) 48
EcoRII CCWGG 1 cut(s) 507
EcoT14I CCWWGG 1 cut(s) 430
ErhI CCWWGG 1 cut(s) 430
FaiI YATR 9 cut(s) 33, 255, 388, 390, 408, 423, 645, 651, 653
FalI AAGNNNNNCTT 2 cut(s) 305, 337
FaqI GGGAC 1 cut(s) 10
FauI CCCGC 2 cut(s) 38, 137
FauNDI CATATG 1 cut(s) 388
FblI GTMKAC 1 cut(s) 107
Fnu4HI GCNGC 4 cut(s) 11, 90, 225, 271
FokI GGATG 3 cut(s) 115, 524, 644
Fsp4HI GCNGC 4 cut(s) 11, 90, 225, 271
FspBI CTAG 1 cut(s) 486
GluI GCNGC 4 cut(s) 11, 90, 225, 271
GsuI CTGGAG 1 cut(s) 629
HapII CCGG 2 cut(s) 66, 111
HgaI GACGC 1 cut(s) 367
HincII GTYRAC 1 cut(s) 108
HindII GTYRAC 1 cut(s) 108
HindIII AAGCTT 1 cut(s) 204
HpaII CCGG 2 cut(s) 66, 111
HphI GGTGA 1 cut(s) 118
Hpy166II GTNNAC 1 cut(s) 108
Hpy188I TCNGA 2 cut(s) 102, 532
Hpy188III TCNNGA 1 cut(s) 464
Hpy8I GTNNAC 1 cut(s) 108
HpyAV CCTTC 2 cut(s) 359, 658
HpyCH4III ACNGT 2 cut(s) 80, 119
HpyCH4V TGCA 4 cut(s) 150, 211, 569, 575
HpyF10VI GCNNNNNNNGC 3 cut(s) 164, 173, 512
HpyF3I CTNAG 3 cut(s) 228, 355, 659
Kzo9I GATC 2 cut(s) 60, 543
LmnI GCTCC 3 cut(s) 68, 431, 655
Lsp1109I GCAGC 3 cut(s) 22, 236, 257
LweI GCATC 5 cut(s) 185, 256, 382, 502, 578
MaeI CTAG 1 cut(s) 486
MaeIII GTNAC 2 cut(s) 113, 517
MalI GATC 2 cut(s) 62, 545
MboI GATC 2 cut(s) 60, 543
MboII GAAGA 3 cut(s) 171, 313, 373
MfeI CAATTG 1 cut(s) 473
MluCI AATT 2 cut(s) 152, 473
MmeI TCCRAC 2 cut(s) 321, 510
MnlI CCTC 3 cut(s) 405, 632, 654
MseI TTAA 1 cut(s) 339
MspA1I CMGCKG 1 cut(s) 526
MspI CCGG 2 cut(s) 66, 111
MspR9I CCNGG 1 cut(s) 509
MunI CAATTG 1 cut(s) 473
MvaI CCWGG 1 cut(s) 509
MvnI CGCG 1 cut(s) 132
MwoI GCNNNNNNNGC 3 cut(s) 164, 173, 512
NdeI CATATG 1 cut(s) 388
NdeII GATC 2 cut(s) 60, 543
NlaIV GGNNCC 5 cut(s) 26, 49, 70, 403, 637
NmuCI GTSAC 1 cut(s) 517
PflFI GACNNNGTC 1 cut(s) 551
PinAI ACCGGT 1 cut(s) 110
PkrI GCNGC 4 cut(s) 12, 91, 226, 272
Ple19I CGATCG 2 cut(s) 63, 546
PpuMI RGGWCCY 1 cut(s) 48
Psp5II RGGWCCY 1 cut(s) 48
Psp6I CCWGG 1 cut(s) 507
PspGI CCWGG 1 cut(s) 507
PspN4I GGNNCC 5 cut(s) 26, 49, 70, 403, 637
PspPI GGNCC 2 cut(s) 24, 48
PspPPI RGGWCCY 1 cut(s) 48
PsyI GACNNNGTC 1 cut(s) 551
PvuI CGATCG 2 cut(s) 63, 546
RgaI GCGATCGC 1 cut(s) 63
SalI GTCGAC 1 cut(s) 106
SaqAI TTAA 1 cut(s) 339
SatI GCNGC 4 cut(s) 11, 90, 225, 271
Sau3AI GATC 2 cut(s) 60, 543
Sau96I GGNCC 2 cut(s) 24, 48
ScrFI CCNGG 1 cut(s) 509
SetI ASST 9 cut(s) 169, 196, 208, 229, 272, 370, 428, 436, 660
SfaAI GCGATCGC 1 cut(s) 63
SfaNI GCATC 5 cut(s) 185, 256, 382, 502, 578
SgfI GCGATCGC 1 cut(s) 63
SinI GGWCC 2 cut(s) 24, 48
Sse9I AATT 2 cut(s) 152, 473
SsiI CCGC 7 cut(s) 45, 90, 130, 158, 316, 524, 629
SspMI CTAG 1 cut(s) 486
StyD4I CCNGG 1 cut(s) 507
StyI CCWWGG 1 cut(s) 430
TaaI ACNGT 2 cut(s) 80, 119
TaqI TCGA 2 cut(s) 107, 546
TasI AATT 2 cut(s) 152, 473
TauI GCSGC 1 cut(s) 92
Tru1I TTAA 1 cut(s) 339
Tru9I TTAA 1 cut(s) 339
TscAI CASTG 1 cut(s) 601
TseFI GTSAC 1 cut(s) 517
TseI GCWGC 3 cut(s) 10, 224, 270
Tsp45I GTSAC 1 cut(s) 517
TspRI CASTG 1 cut(s) 601
Tth111I GACNNNGTC 1 cut(s) 551
VpaK11BI GGWCC 2 cut(s) 24, 48
XagI CCTNNNNNAGG 1 cut(s) 409
XmiI GTMKAC 1 cut(s) 107
XspI CTAG 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.