Rh1DG223800

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0010086
Physical Location & Seq
Reverse (-)
43384957 .. 43388485
3529 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG223800.1

Sequence Viewer

Length: 501 bp
ATGGCCTCTTCTGGTTTTCAAGCTGATGTGAAAGCTTTACAGAAGCATTTGACAGCTAAGCACTTGATTTATCAGCATCAGCATAACAAACAAATGAGCTGCCCTGCAATGGCTCAACTACTTTCCAATACTCTTTACTACAAACGCTTCTTTCCCTATTACGCCTTTAATGTTTTGGGTGGTCTAGACACTGAAGGGAAGGGTTGTGTCTTCACATATGATGCTGTTGGTTCCTATGAGAGGGTTGGATATAGTGCCCAAGGTTCTGGTTCCACACTCATCATGCCTTTCTTGGATAACCAACTGAAGTCTCCCAGTCCTCTCTTGTTGCCTGCCGTGGATGCTGTAACACCACTTTCAGAAGGAGAAGCAATTGATTTGGTCAAAACTGTTTTTGCATCTGCCACTGAGAGGGATATCTACACTGGAGACAGGCTTGAAATTTATGTCCTAAATGCTCATGGCACTCATCATGAATACATGGAACTGAGGAAAGATTGA

Protein Analysis

166

Amino Acids

18.46

Weight (kDa)

6.14

Isoelectric Point (pI)

43.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 1 - 150 1.2e-21 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010086)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G60820 AT3G60820 AT3G60820
fragaria_vesca FvH4_7g11920 FvH4_7g11920
malus_domestica MD02G1204000.v1.1 MD07G1122000.v1.1
prunus_persica Prupe.2G145800_v2.0.a1
pyrus_communis pycom02g16630 pycom07g11030
rosa_chinensis RchiOBHm_Chr1g0350601
rosa_laevigata RLG00000028469
rosa_multiflora Rmu_sc0014368.1_g000004 Rmu_sc0030263.1_g000001
rosa_roxburghii Rroxscaffold_4G00304680
rosa_rugosa Rorug01G0212500
rosa_samantha Rh1AG227000 Rh1BG195700 Rh1CG211800 Rh1DG223800
rosa_wichuraiana Rw1G019620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 441
AcuI CTGAAG 2 cut(s) 213, 326
AfiI CCNNNNNNNGG 3 cut(s) 109, 240, 411
AgsI TTSAA 2 cut(s) 20, 440
AluBI AGCT 4 cut(s) 23, 35, 56, 99
AluI AGCT 4 cut(s) 23, 35, 56, 99
Alw26I GTCTC 2 cut(s) 315, 423
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 99
ApoI RAATTY 1 cut(s) 441
BaeGI GKGCMC 1 cut(s) 259
BbsI GAAGAC 1 cut(s) 202
BbvI GCAGC 1 cut(s) 86
BceAI ACGGC 1 cut(s) 320
BcoDI GTCTC 2 cut(s) 315, 423
BfaI CTAG 1 cut(s) 185
BisI GCNGC 1 cut(s) 100
BlpI GCTNAGC 1 cut(s) 57
BlsI GCNGC 1 cut(s) 101
BmiI GGNNCC 2 cut(s) 232, 271
BmrI ACTGGG 1 cut(s) 309
BmsI GCATC 4 cut(s) 85, 211, 331, 407
BmuI ACTGGG 1 cut(s) 309
BpiI GAAGAC 1 cut(s) 202
BpmI CTGGAG 1 cut(s) 447
Bpu1102I GCTNAGC 1 cut(s) 57
BsaJI CCNNGG 2 cut(s) 259, 336
Bsc4I CCNNNNNNNGG 3 cut(s) 109, 240, 411
Bse1I ACTGG 2 cut(s) 315, 430
Bse3DI GCAATG 1 cut(s) 114
BseDI CCNNGG 2 cut(s) 259, 336
BseGI GGATG 1 cut(s) 346
BseLI CCNNNNNNNGG 3 cut(s) 109, 240, 411
BseMI GCAATG 1 cut(s) 114
BseMII CTCAG 2 cut(s) 399, 479
BseNI ACTGG 2 cut(s) 315, 430
BseSI GKGCMC 1 cut(s) 259
BseXI GCAGC 1 cut(s) 86
BshFI GGCC 1 cut(s) 5
BslI CCNNNNNNNGG 3 cut(s) 109, 240, 411
BsmAI GTCTC 2 cut(s) 315, 423
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 259
Bsp1720I GCTNAGC 1 cut(s) 57
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 400, 480
BspHI TCATGA 1 cut(s) 472
BspLI GGNNCC 2 cut(s) 232, 271
BsrDI GCAATG 1 cut(s) 114
BsrI ACTGG 2 cut(s) 315, 430
BssECI CCNNGG 2 cut(s) 259, 336
BssT1I CCWWGG 1 cut(s) 259
Bst4CI ACNGT 1 cut(s) 391
Bst6I CTCTTC 1 cut(s) 13
BstC8I GCNNGC 1 cut(s) 333
BstDEI CTNAG 3 cut(s) 57, 408, 488
BstDSI CCRYGG 1 cut(s) 336
BstENI CCTNNNNNAGG 1 cut(s) 238
BstF5I GGATG 1 cut(s) 346
BstMAI GTCTC 2 cut(s) 315, 423
BstMWI GCNNNNNNNGC 1 cut(s) 341
BstSLI GKGCMC 1 cut(s) 259
BstV1I GCAGC 1 cut(s) 86
BstV2I GAAGAC 1 cut(s) 202
BstXI CCANNNNNNTGG 1 cut(s) 266
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 336
BtsCI GGATG 1 cut(s) 346
BtsIMutI CAGTG 3 cut(s) 189, 405, 423
Cac8I GCNNGC 1 cut(s) 333
CciI TCATGA 1 cut(s) 472
CviAII CATG 4 cut(s) 283, 461, 473, 481
CviJI RGCY 7 cut(s) 5, 23, 35, 56, 99, 113, 436
CviKI_1 RGCY 7 cut(s) 5, 23, 35, 56, 99, 113, 436
DdeI CTNAG 3 cut(s) 57, 408, 488
Eam1104I CTCTTC 1 cut(s) 13
EarI CTCTTC 1 cut(s) 13
Eco130I CCWWGG 1 cut(s) 259
Eco32I GATATC 1 cut(s) 418
Eco57I CTGAAG 2 cut(s) 213, 326
EcoNI CCTNNNNNAGG 1 cut(s) 238
EcoRV GATATC 1 cut(s) 418
EcoT14I CCWWGG 1 cut(s) 259
ErhI CCWWGG 1 cut(s) 259
FaeI CATG 4 cut(s) 286, 464, 476, 484
FatI CATG 4 cut(s) 282, 460, 472, 480
FauNDI CATATG 1 cut(s) 217
Fnu4HI GCNGC 1 cut(s) 100
FokI GGATG 1 cut(s) 353
Fsp4HI GCNGC 1 cut(s) 100
FspBI CTAG 1 cut(s) 185
GluI GCNGC 1 cut(s) 100
GsuI CTGGAG 1 cut(s) 447
HaeIII GGCC 1 cut(s) 5
Hin1II CATG 4 cut(s) 286, 464, 476, 484
HindIII AAGCTT 1 cut(s) 33
Hpy188I TCNGA 1 cut(s) 361
Hpy188III TCNNGA 2 cut(s) 185, 473
HpyAV CCTTC 3 cut(s) 188, 193, 356
HpyCH4III ACNGT 1 cut(s) 391
HpyCH4V TGCA 2 cut(s) 107, 398
HpyF10VI GCNNNNNNNGC 1 cut(s) 341
HpyF3I CTNAG 3 cut(s) 57, 408, 488
Hsp92II CATG 4 cut(s) 286, 464, 476, 484
LpnPI CCDG 6 cut(s) 117, 252, 328, 345, 411, 418
Lsp1109I GCAGC 1 cut(s) 86
LweI GCATC 4 cut(s) 85, 211, 331, 407
MaeI CTAG 1 cut(s) 185
MaeIII GTNAC 1 cut(s) 346
MboII GAAGA 1 cut(s) 202
MfeI CAATTG 1 cut(s) 372
MhlI GDGCHC 1 cut(s) 259
MluCI AATT 2 cut(s) 372, 441
MmeI TCCRAC 1 cut(s) 226
MnlI CCTC 5 cut(s) 16, 234, 330, 405, 483
MseI TTAA 1 cut(s) 168
MunI CAATTG 1 cut(s) 372
MwoI GCNNNNNNNGC 1 cut(s) 341
NdeI CATATG 1 cut(s) 217
NlaIII CATG 4 cut(s) 286, 464, 476, 484
NlaIV GGNNCC 2 cut(s) 232, 271
PagI TCATGA 1 cut(s) 472
PkrI GCNGC 1 cut(s) 101
PspN4I GGNNCC 2 cut(s) 232, 271
SaqAI TTAA 1 cut(s) 168
SatI GCNGC 1 cut(s) 100
SduI GDGCHC 1 cut(s) 259
SetI ASST 5 cut(s) 25, 37, 58, 101, 265
SfaNI GCATC 4 cut(s) 85, 211, 331, 407
Sse9I AATT 2 cut(s) 372, 441
SspMI CTAG 1 cut(s) 185
StyI CCWWGG 1 cut(s) 259
TaaI ACNGT 1 cut(s) 391
TasI AATT 2 cut(s) 372, 441
Tru1I TTAA 1 cut(s) 168
Tru9I TTAA 1 cut(s) 168
TscAI CASTG 3 cut(s) 196, 412, 430
TseI GCWGC 1 cut(s) 99
TspDTI ATGAA 1 cut(s) 489
TspRI CASTG 3 cut(s) 196, 412, 430
XagI CCTNNNNNAGG 1 cut(s) 238
XapI RAATTY 1 cut(s) 441
XbaI TCTAGA 1 cut(s) 184
XspI CTAG 1 cut(s) 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.