Rh1BG195700

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0010086
Physical Location & Seq
Reverse (-)
31221065 .. 31224384
3320 bp
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UTR
Exon/CDS
Intron
Rh1BG195700.1

Sequence Viewer

Length: 570 bp
ATGTCGACGGGTTACAGTATTCTCACCCGCGGCTACTCCAAAATCAGCAAATTAGCGGACAAAGCTTTTATGGCCTCTTCTGGTTTTCAAGCTGATGTGAAAGCTTTACAGAAGCATTTGACAGCTAAGCACTTGATTTATCAGCATCAGCATAACAAACAAATGAGCTGCCCTGCAATGGCTCAACTACTTTCCAATACTCTTTACTACAAACGCTTCTTTCCCTATTACGCCTTTAATGTTTTGGGTGGTCTAGACACTGAAGGGAAGGGTTGTGTCTTCACATATGATGCTGTTGGTTCCTATGAGAGGGTTGGATATAGTGCCCAAGGTTCTGGTTCCACACTCATCATGCCTTTCTTGGATAACCAACTGAAGTCTCCCAGTCCTCTCTTGTTGCCTGCCGTGGATGCTGTAACACCACTTTCAGAAGGAGAAGCAATTGATTTGGTCAAAACTGTTTTTGCATCTGCCACTGAGAGGGATATCTACACTGGAGACAGGCTTGAAATTTATGTCCTAAATGCTCATGGCACTCATCATGAATACATGGAACTGAGGAAAGATTGA

Protein Analysis

189

Amino Acids

20.98

Weight (kDa)

7.05

Isoelectric Point (pI)

38.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 2 - 173 7.8e-27 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010086)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G60820 AT3G60820 AT3G60820
fragaria_vesca FvH4_7g11920 FvH4_7g11920
malus_domestica MD02G1204000.v1.1 MD07G1122000.v1.1
prunus_persica Prupe.2G145800_v2.0.a1
pyrus_communis pycom02g16630 pycom07g11030
rosa_chinensis RchiOBHm_Chr1g0350601
rosa_laevigata RLG00000028469
rosa_multiflora Rmu_sc0014368.1_g000004 Rmu_sc0030263.1_g000001
rosa_roxburghii Rroxscaffold_4G00304680
rosa_rugosa Rorug01G0212500
rosa_samantha Rh1AG227000 Rh1BG195700 Rh1CG211800 Rh1DG223800
rosa_wichuraiana Rw1G019620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 30
AciI CCGC 3 cut(s) 28, 30, 56
AcsI RAATTY 1 cut(s) 510
AcuI CTGAAG 2 cut(s) 282, 395
AfiI CCNNNNNNNGG 3 cut(s) 178, 309, 480
AgsI TTSAA 2 cut(s) 89, 509
AluBI AGCT 5 cut(s) 65, 92, 104, 125, 168
AluI AGCT 5 cut(s) 65, 92, 104, 125, 168
Alw26I GTCTC 2 cut(s) 384, 492
AoxI GGCC 1 cut(s) 72
ApeKI GCWGC 1 cut(s) 168
ApoI RAATTY 1 cut(s) 510
AsuHPI GGTGA 1 cut(s) 16
BaeGI GKGCMC 1 cut(s) 328
BbsI GAAGAC 1 cut(s) 271
BbvI GCAGC 1 cut(s) 155
BceAI ACGGC 1 cut(s) 389
BcoDI GTCTC 2 cut(s) 384, 492
BfaI CTAG 1 cut(s) 254
BisI GCNGC 2 cut(s) 31, 169
BlpI GCTNAGC 1 cut(s) 126
BlsI GCNGC 2 cut(s) 32, 170
BmiI GGNNCC 2 cut(s) 301, 340
BmrI ACTGGG 1 cut(s) 378
BmsI GCATC 4 cut(s) 154, 280, 400, 476
BmuI ACTGGG 1 cut(s) 378
BpiI GAAGAC 1 cut(s) 271
BpmI CTGGAG 1 cut(s) 516
Bpu1102I GCTNAGC 1 cut(s) 126
BsaJI CCNNGG 3 cut(s) 28, 328, 405
Bsc4I CCNNNNNNNGG 3 cut(s) 178, 309, 480
Bse1I ACTGG 2 cut(s) 384, 499
Bse3DI GCAATG 1 cut(s) 183
BseDI CCNNGG 3 cut(s) 28, 328, 405
BseGI GGATG 1 cut(s) 415
BseLI CCNNNNNNNGG 3 cut(s) 178, 309, 480
BseMI GCAATG 1 cut(s) 183
BseMII CTCAG 2 cut(s) 468, 548
BseNI ACTGG 2 cut(s) 384, 499
BseSI GKGCMC 1 cut(s) 328
BseXI GCAGC 1 cut(s) 155
Bsh1236I CGCG 1 cut(s) 30
BshFI GGCC 1 cut(s) 74
BslI CCNNNNNNNGG 3 cut(s) 178, 309, 480
BsmAI GTCTC 2 cut(s) 384, 492
BsnI GGCC 1 cut(s) 74
Bsp1286I GDGCHC 1 cut(s) 328
Bsp1720I GCTNAGC 1 cut(s) 126
BspACI CCGC 3 cut(s) 28, 30, 56
BspANI GGCC 1 cut(s) 74
BspCNI CTCAG 2 cut(s) 469, 549
BspFNI CGCG 1 cut(s) 30
BspHI TCATGA 1 cut(s) 541
BspLI GGNNCC 2 cut(s) 301, 340
BsrDI GCAATG 1 cut(s) 183
BsrI ACTGG 2 cut(s) 384, 499
BssECI CCNNGG 3 cut(s) 28, 328, 405
BssT1I CCWWGG 1 cut(s) 328
Bst4CI ACNGT 2 cut(s) 17, 460
Bst6I CTCTTC 1 cut(s) 82
BstC8I GCNNGC 1 cut(s) 402
BstDEI CTNAG 3 cut(s) 126, 477, 557
BstDSI CCRYGG 2 cut(s) 28, 405
BstENI CCTNNNNNAGG 1 cut(s) 307
BstF5I GGATG 1 cut(s) 415
BstFNI CGCG 1 cut(s) 30
BstMAI GTCTC 2 cut(s) 384, 492
BstMWI GCNNNNNNNGC 3 cut(s) 62, 71, 410
BstSLI GKGCMC 1 cut(s) 328
BstUI CGCG 1 cut(s) 30
BstV1I GCAGC 1 cut(s) 155
BstV2I GAAGAC 1 cut(s) 271
BstXI CCANNNNNNTGG 1 cut(s) 335
BsuRI GGCC 1 cut(s) 74
BtgI CCRYGG 2 cut(s) 28, 405
BtsCI GGATG 1 cut(s) 415
BtsIMutI CAGTG 3 cut(s) 258, 474, 492
Cac8I GCNNGC 1 cut(s) 402
CciI TCATGA 1 cut(s) 541
Cfr42I CCGCGG 1 cut(s) 31
CviAII CATG 4 cut(s) 352, 530, 542, 550
CviJI RGCY 9 cut(s) 33, 65, 74, 92, 104, 125, 168, 182, 505
CviKI_1 RGCY 9 cut(s) 33, 65, 74, 92, 104, 125, 168, 182, 505
DdeI CTNAG 3 cut(s) 126, 477, 557
Eam1104I CTCTTC 1 cut(s) 82
EarI CTCTTC 1 cut(s) 82
Eco130I CCWWGG 1 cut(s) 328
Eco32I GATATC 1 cut(s) 487
Eco57I CTGAAG 2 cut(s) 282, 395
EcoNI CCTNNNNNAGG 1 cut(s) 307
EcoRV GATATC 1 cut(s) 487
EcoT14I CCWWGG 1 cut(s) 328
ErhI CCWWGG 1 cut(s) 328
FaeI CATG 4 cut(s) 355, 533, 545, 553
FatI CATG 4 cut(s) 351, 529, 541, 549
FauI CCCGC 1 cut(s) 35
FauNDI CATATG 1 cut(s) 286
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 2 cut(s) 31, 169
FokI GGATG 1 cut(s) 422
Fsp4HI GCNGC 2 cut(s) 31, 169
FspBI CTAG 1 cut(s) 254
GluI GCNGC 2 cut(s) 31, 169
GsuI CTGGAG 1 cut(s) 516
HaeIII GGCC 1 cut(s) 74
Hin1II CATG 4 cut(s) 355, 533, 545, 553
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 2 cut(s) 63, 102
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 1 cut(s) 430
Hpy188III TCNNGA 2 cut(s) 254, 542
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 3 cut(s) 257, 262, 425
HpyCH4III ACNGT 2 cut(s) 17, 460
HpyCH4V TGCA 2 cut(s) 176, 467
HpyF10VI GCNNNNNNNGC 3 cut(s) 62, 71, 410
HpyF3I CTNAG 3 cut(s) 126, 477, 557
Hsp92II CATG 4 cut(s) 355, 533, 545, 553
KspI CCGCGG 1 cut(s) 31
LpnPI CCDG 7 cut(s) 66, 186, 321, 397, 414, 480, 487
Lsp1109I GCAGC 1 cut(s) 155
LweI GCATC 4 cut(s) 154, 280, 400, 476
MaeI CTAG 1 cut(s) 254
MaeIII GTNAC 2 cut(s) 11, 415
MboII GAAGA 2 cut(s) 69, 271
MfeI CAATTG 1 cut(s) 441
MhlI GDGCHC 1 cut(s) 328
MluCI AATT 3 cut(s) 50, 441, 510
MmeI TCCRAC 1 cut(s) 295
MnlI CCTC 5 cut(s) 85, 303, 399, 474, 552
MseI TTAA 1 cut(s) 237
MspA1I CMGCKG 1 cut(s) 30
MunI CAATTG 1 cut(s) 441
MvnI CGCG 1 cut(s) 30
MwoI GCNNNNNNNGC 3 cut(s) 62, 71, 410
NdeI CATATG 1 cut(s) 286
NlaIII CATG 4 cut(s) 355, 533, 545, 553
NlaIV GGNNCC 2 cut(s) 301, 340
PagI TCATGA 1 cut(s) 541
PkrI GCNGC 2 cut(s) 32, 170
PspN4I GGNNCC 2 cut(s) 301, 340
SacII CCGCGG 1 cut(s) 31
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 1 cut(s) 237
SatI GCNGC 2 cut(s) 31, 169
SduI GDGCHC 1 cut(s) 328
SetI ASST 6 cut(s) 67, 94, 106, 127, 170, 334
SfaNI GCATC 4 cut(s) 154, 280, 400, 476
Sfr303I CCGCGG 1 cut(s) 31
SgrBI CCGCGG 1 cut(s) 31
Sse9I AATT 3 cut(s) 50, 441, 510
SsiI CCGC 3 cut(s) 28, 30, 56
SspMI CTAG 1 cut(s) 254
StyI CCWWGG 1 cut(s) 328
TaaI ACNGT 2 cut(s) 17, 460
TaqI TCGA 1 cut(s) 5
TasI AATT 3 cut(s) 50, 441, 510
TauI GCSGC 1 cut(s) 33
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
TscAI CASTG 3 cut(s) 265, 481, 499
TseI GCWGC 1 cut(s) 168
TspDTI ATGAA 1 cut(s) 558
TspRI CASTG 3 cut(s) 265, 481, 499
XagI CCTNNNNNAGG 1 cut(s) 307
XapI RAATTY 1 cut(s) 510
XbaI TCTAGA 1 cut(s) 253
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.