MD07G1122000.v1.1

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0010086
Physical Location & Seq
Reverse (-)
15606015 .. 15609305
3291 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1122000.v1.1.491

Sequence Viewer

Length: 672 bp
ATGACAAAGCAGCACGCTAACTGGTCCCCCTACGACAACAATGGCGGATCCTGCGTTGCGATCGCCGGAGCCAACTACTGTGTGATTGCTGCCGATACTCGGATGTCGACTGGTTACAGTATTCTCACCCGCGACTACTCCAAAATCTGCAAATTAGCGGACAAAGCTGTATTGGCATCTTCAGGTTTCCAAGCTGATGTGAAAGCTTTGCAAAAGCATTTGGCAGCTAAGCACTTGACGTATCAGCATCAACATAACAAACAAATGAGCTGCCCTGCAATGGCTCAATTGCTTTCCAACACCCTTTACTACAAGCGTTTCTTCCCCTACTACGCTTTTAATGTTTTGGGTGGTTTAGATAGTGAAGGTAAGGGTTGTGTATTCACATACGACGCTGTTGGTTCCTACGAGAGGGTTGGGTATAGCTCCCAAGGTTCTGGTTCTACACTTATCATTCCTTTCCTGGACAACCAATTGAAGTCTCCTAGCCCGCTCCTATTGCCTGCCCAGGATGCTGTCACACCGCTGTCTGAAGCAGAAGCAATCGACTTGGTCAAAACTTGTTTTGCATCTGCAACCGAAAGAGATATTTACACTGGTGACAGCTTGGAGATTGTTGTCCTGAATGCGGATGGCACCCGGTACGAGTATATGGAGCTGAGGAAGGACTGA

Protein Analysis

224

Amino Acids

24.45

Weight (kDa)

6.2

Isoelectric Point (pI)

41.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 13 - 207 1.9e-36 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010086)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G60820 AT3G60820 AT3G60820
fragaria_vesca FvH4_7g11920 FvH4_7g11920
malus_domestica MD02G1204000.v1.1 MD07G1122000.v1.1
prunus_persica Prupe.2G145800_v2.0.a1
pyrus_communis pycom02g16630 pycom07g11030
rosa_chinensis RchiOBHm_Chr1g0350601
rosa_laevigata RLG00000028469
rosa_multiflora Rmu_sc0014368.1_g000004 Rmu_sc0030263.1_g000001
rosa_roxburghii Rroxscaffold_4G00304680
rosa_rugosa Rorug01G0212500
rosa_samantha Rh1AG227000 Rh1BG195700 Rh1CG211800 Rh1DG223800
rosa_wichuraiana Rw1G019620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 635
AccBSI CCGCTC 1 cut(s) 493
AccI GTMKAC 1 cut(s) 107
AccII CGCG 1 cut(s) 132
AciI CCGC 6 cut(s) 45, 130, 158, 491, 524, 629
AclWI GGATC 2 cut(s) 42, 55
AcuI CTGAAG 2 cut(s) 165, 552
AfaI GTAC 1 cut(s) 644
AfiI CCNNNNNNNGG 4 cut(s) 99, 280, 411, 628
AgsI TTSAA 1 cut(s) 478
AjnI CCWGG 2 cut(s) 462, 507
AluBI AGCT 8 cut(s) 167, 194, 206, 227, 270, 426, 606, 658
AluI AGCT 8 cut(s) 167, 194, 206, 227, 270, 426, 606, 658
Alw26I GTCTC 1 cut(s) 486
AlwI GGATC 2 cut(s) 42, 55
ApeKI GCWGC 4 cut(s) 10, 89, 224, 270
AsiSI GCGATCGC 1 cut(s) 63
AspS9I GGNCC 1 cut(s) 24
AsuC2I CCSGG 1 cut(s) 640
AsuHPI GGTGA 2 cut(s) 118, 611
AvaII GGWCC 1 cut(s) 24
BamHI GGATCC 1 cut(s) 47
BanI GGYRCC 1 cut(s) 635
BbvCI CCTCAGC 1 cut(s) 659
BbvI GCAGC 4 cut(s) 22, 76, 236, 257
BccI CCATC 1 cut(s) 626
BciT130I CCWGG 2 cut(s) 464, 509
BcnI CCSGG 1 cut(s) 640
BcoDI GTCTC 1 cut(s) 486
BfaI CTAG 1 cut(s) 486
BisI GCNGC 4 cut(s) 11, 90, 225, 271
BlpI GCTNAGC 1 cut(s) 228
BlsI GCNGC 4 cut(s) 12, 91, 226, 272
Bme1390I CCNGG 3 cut(s) 464, 509, 640
Bme18I GGWCC 1 cut(s) 24
BmgT120I GGNCC 1 cut(s) 24
BmiI GGNNCC 5 cut(s) 26, 49, 70, 403, 637
BmrFI CCNGG 3 cut(s) 464, 509, 640
BmsI GCATC 4 cut(s) 185, 256, 502, 578
Bpu10I CCTNAGC 1 cut(s) 659
Bpu1102I GCTNAGC 1 cut(s) 228
BpuMI CCSGG 1 cut(s) 640
BsaJI CCNNGG 2 cut(s) 430, 507
Bsc4I CCNNNNNNNGG 4 cut(s) 99, 280, 411, 628
Bse1I ACTGG 3 cut(s) 26, 115, 601
Bse3DI GCAATG 1 cut(s) 285
BseBI CCWGG 2 cut(s) 464, 509
BseDI CCNNGG 2 cut(s) 430, 507
BseGI GGATG 3 cut(s) 108, 517, 637
BseLI CCNNNNNNNGG 4 cut(s) 99, 280, 411, 628
BseMI GCAATG 1 cut(s) 285
BseMII CTCAG 1 cut(s) 650
BseNI ACTGG 3 cut(s) 26, 115, 601
BseXI GCAGC 4 cut(s) 22, 76, 236, 257
Bsh1236I CGCG 1 cut(s) 132
Bsh1285I CGRYCG 1 cut(s) 63
BshNI GGYRCC 1 cut(s) 635
BsiEI CGRYCG 1 cut(s) 63
BsiSI CCGG 2 cut(s) 66, 640
BslFI GGGAC 1 cut(s) 10
BslI CCNNNNNNNGG 4 cut(s) 99, 280, 411, 628
BsmAI GTCTC 1 cut(s) 486
BsmFI GGGAC 1 cut(s) 10
BsmI GAATGC 1 cut(s) 631
Bsp143I GATC 2 cut(s) 47, 60
Bsp1720I GCTNAGC 1 cut(s) 228
BspACI CCGC 6 cut(s) 45, 130, 158, 491, 524, 629
BspCNI CTCAG 1 cut(s) 651
BspFNI CGCG 1 cut(s) 132
BspLI GGNNCC 5 cut(s) 26, 49, 70, 403, 637
BspPI GGATC 2 cut(s) 42, 55
BspT107I GGYRCC 1 cut(s) 635
BsrBI CCGCTC 1 cut(s) 493
BsrDI GCAATG 1 cut(s) 285
BsrI ACTGG 3 cut(s) 26, 115, 601
BssECI CCNNGG 2 cut(s) 430, 507
BssMI GATC 2 cut(s) 47, 60
BssT1I CCWWGG 1 cut(s) 430
Bst2UI CCWGG 2 cut(s) 464, 509
Bst4CI ACNGT 2 cut(s) 80, 119
BstC8I GCNNGC 3 cut(s) 15, 491, 504
BstDEI CTNAG 2 cut(s) 228, 659
BstENI CCTNNNNNAGG 1 cut(s) 409
BstF5I GGATG 3 cut(s) 108, 517, 637
BstFNI CGCG 1 cut(s) 132
BstKTI GATC 2 cut(s) 50, 63
BstMAI GTCTC 1 cut(s) 486
BstMBI GATC 2 cut(s) 47, 60
BstMCI CGRYCG 1 cut(s) 63
BstMWI GCNNNNNNNGC 5 cut(s) 51, 164, 173, 499, 512
BstNI CCWGG 2 cut(s) 464, 509
BstSCI CCNGG 3 cut(s) 462, 507, 638
BstUI CGCG 1 cut(s) 132
BstV1I GCAGC 4 cut(s) 22, 76, 236, 257
BstX2I RGATCY 1 cut(s) 47
BstXI CCANNNNNNTGG 1 cut(s) 437
BstYI RGATCY 1 cut(s) 47
BtsCI GGATG 3 cut(s) 108, 517, 637
BtsIMutI CAGTG 1 cut(s) 594
Cac8I GCNNGC 3 cut(s) 15, 491, 504
Cfr13I GGNCC 1 cut(s) 24
CseI GACGC 1 cut(s) 401
Csp6I GTAC 1 cut(s) 643
CviQI GTAC 1 cut(s) 643
DdeI CTNAG 2 cut(s) 228, 659
DpnI GATC 2 cut(s) 49, 62
DpnII GATC 2 cut(s) 47, 60
EciI GGCGGA 1 cut(s) 60
Eco130I CCWWGG 1 cut(s) 430
Eco47I GGWCC 1 cut(s) 24
Eco57I CTGAAG 2 cut(s) 165, 552
EcoNI CCTNNNNNAGG 1 cut(s) 409
EcoRII CCWGG 2 cut(s) 462, 507
EcoT14I CCWWGG 1 cut(s) 430
ErhI CCWWGG 1 cut(s) 430
FaiI YATR 5 cut(s) 255, 388, 423, 651, 653
FalI AAGNNNNNCTT 2 cut(s) 305, 337
FaqI GGGAC 1 cut(s) 10
FauI CCCGC 2 cut(s) 137, 498
FblI GTMKAC 1 cut(s) 107
Fnu4HI GCNGC 4 cut(s) 11, 90, 225, 271
FokI GGATG 3 cut(s) 115, 524, 644
Fsp4HI GCNGC 4 cut(s) 11, 90, 225, 271
FspBI CTAG 1 cut(s) 486
GluI GCNGC 4 cut(s) 11, 90, 225, 271
HapII CCGG 2 cut(s) 66, 640
HgaI GACGC 1 cut(s) 401
HincII GTYRAC 1 cut(s) 108
HindII GTYRAC 1 cut(s) 108
HindIII AAGCTT 1 cut(s) 204
HpaII CCGG 2 cut(s) 66, 640
HphI GGTGA 2 cut(s) 118, 611
Hpy166II GTNNAC 1 cut(s) 108
Hpy188I TCNGA 2 cut(s) 102, 532
Hpy188III TCNNGA 1 cut(s) 622
Hpy8I GTNNAC 1 cut(s) 108
Hpy99I CGWCG 1 cut(s) 395
HpyAV CCTTC 2 cut(s) 359, 658
HpyCH4III ACNGT 2 cut(s) 80, 119
HpyCH4IV ACGT 1 cut(s) 239
HpyCH4V TGCA 5 cut(s) 150, 211, 278, 569, 575
HpyF10VI GCNNNNNNNGC 5 cut(s) 51, 164, 173, 499, 512
HpyF3I CTNAG 2 cut(s) 228, 659
HpySE526I ACGT 1 cut(s) 239
Kzo9I GATC 2 cut(s) 47, 60
LmnI GCTCC 4 cut(s) 68, 431, 498, 655
Lsp1109I GCAGC 4 cut(s) 22, 76, 236, 257
LweI GCATC 4 cut(s) 185, 256, 502, 578
MaeI CTAG 1 cut(s) 486
MaeII ACGT 1 cut(s) 239
MaeIII GTNAC 3 cut(s) 113, 517, 599
MalI GATC 2 cut(s) 49, 62
MbiI CCGCTC 1 cut(s) 493
MboI GATC 2 cut(s) 47, 60
MboII GAAGA 2 cut(s) 171, 313
MfeI CAATTG 2 cut(s) 287, 473
MflI RGATCY 1 cut(s) 47
MluCI AATT 3 cut(s) 152, 287, 473
MmeI TCCRAC 1 cut(s) 321
MnlI CCTC 2 cut(s) 405, 654
MseI TTAA 1 cut(s) 339
MspA1I CMGCKG 1 cut(s) 526
MspI CCGG 2 cut(s) 66, 640
MspR9I CCNGG 3 cut(s) 464, 509, 640
MunI CAATTG 2 cut(s) 287, 473
Mva1269I GAATGC 1 cut(s) 631
MvaI CCWGG 2 cut(s) 464, 509
MvnI CGCG 1 cut(s) 132
MwoI GCNNNNNNNGC 5 cut(s) 51, 164, 173, 499, 512
NciI CCSGG 1 cut(s) 640
NdeII GATC 2 cut(s) 47, 60
NlaIV GGNNCC 5 cut(s) 26, 49, 70, 403, 637
NmuCI GTSAC 2 cut(s) 517, 599
PctI GAATGC 1 cut(s) 631
PflFI GACNNNGTC 1 cut(s) 551
PfoI TCCNGGA 1 cut(s) 462
PkrI GCNGC 4 cut(s) 12, 91, 226, 272
Ple19I CGATCG 1 cut(s) 63
Psp6I CCWGG 2 cut(s) 462, 507
PspGI CCWGG 2 cut(s) 462, 507
PspN4I GGNNCC 5 cut(s) 26, 49, 70, 403, 637
PspPI GGNCC 1 cut(s) 24
PsuI RGATCY 1 cut(s) 47
PsyI GACNNNGTC 1 cut(s) 551
PvuI CGATCG 1 cut(s) 63
RgaI GCGATCGC 1 cut(s) 63
RsaI GTAC 1 cut(s) 644
RsaNI GTAC 1 cut(s) 643
SalI GTCGAC 1 cut(s) 106
SaqAI TTAA 1 cut(s) 339
SatI GCNGC 4 cut(s) 11, 90, 225, 271
Sau3AI GATC 2 cut(s) 47, 60
Sau96I GGNCC 1 cut(s) 24
ScrFI CCNGG 3 cut(s) 464, 509, 640
SfaAI GCGATCGC 1 cut(s) 63
SfaNI GCATC 4 cut(s) 185, 256, 502, 578
SgfI GCGATCGC 1 cut(s) 63
SinI GGWCC 1 cut(s) 24
Sse9I AATT 3 cut(s) 152, 287, 473
SsiI CCGC 6 cut(s) 45, 130, 158, 491, 524, 629
SspMI CTAG 1 cut(s) 486
StyD4I CCNGG 3 cut(s) 462, 507, 638
StyI CCWWGG 1 cut(s) 430
TaaI ACNGT 2 cut(s) 80, 119
TaiI ACGT 1 cut(s) 242
TaqI TCGA 2 cut(s) 107, 546
TasI AATT 3 cut(s) 152, 287, 473
Tru1I TTAA 1 cut(s) 339
Tru9I TTAA 1 cut(s) 339
TscAI CASTG 1 cut(s) 601
TseFI GTSAC 2 cut(s) 517, 599
TseI GCWGC 4 cut(s) 10, 89, 224, 270
Tsp45I GTSAC 2 cut(s) 517, 599
TspRI CASTG 1 cut(s) 601
Tth111I GACNNNGTC 1 cut(s) 551
VpaK11BI GGWCC 1 cut(s) 24
XagI CCTNNNNNAGG 1 cut(s) 409
XmiI GTMKAC 1 cut(s) 107
XspI CTAG 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.