AT3G62860

Caffeoylshikimate esterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0006691
Physical Location & Seq
Reverse (-)
23239264 .. 23242274
3011 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G62860.1

Sequence Viewer

Length: 1047 bp
ATGATGAAGTTCAAGTTCCAGTACGAGGAGGAATACATCAAAAACTCGAGAGACGTTGAGTTGTTCGCTTGCAGATGGCTTCCTTCTTCTTCTCCCCGAGCTCTAGTCTTCCTCTGCCATGGTTATGGAATGGAGTGCAGTAGCTTCATGAGAGAATGCGGAATACGGCTGGCATCAGCGGGATATGCAGTGTTTGGGATGGACTACGAGGGGCATGGTCGGTCCAAGGGTGCTCGTTGCTATATAAAAAAATTCAGTAATATAGTAAATGACTGCTTTGACTATTACACATCCATCTCTGCACAAGAAGAATACAAAGAGAAAGGTAGATTTTTGTATGGAGAATCAATGGGAGGTGCAGTTGCTTTGTTGCTTCACAAGAAAGATCCTTCTTTTTGGAACGGGGCTCTTCTCGTTGCTCCCATGTGTAAGATATCAGAGAAGGTGAAACCACACCCAGTAGTCATAAATCTACTAACGAGAGTTGAAGATATTATACCAAAATGGAAGATAGTTCCTACAAAAGATGTTATCGATGCTGCCTTCAAGGATCCGGTCAAGCGCGAAGAGATAAGGAACAACAAGCTGATATATCAAGACAAGCCAAGACTTAAAACCGCACTTGAAATGCTTAGAACGAGCATGGACCTTGAAGACACTCTCCACGAGATCACATTGCCTTTCTTTGTCCTTCACGGTGAAGCAGACATTGTGACTGATCCAGAGATAAGCAAAGCCTTGTTCGAGAAGGCAAGCACACGCGACAAAACCATTAAACTTTACCCAGGAATGTGGCATGGATTAACTTCTGGTGAGCCTGATGCTAACGTTGACCTCGTTTTTGCTGACATTGTCAATTGGCTTGATGCTCGTACCGGCGACTCCGCTTCTCTGACAGTCACTCCCGTACATGATTTTACCTCCAATGTTCAAAAGGTCGTTGACGGTGTTAGTAACGGTCAAGGAAAATCTAAACGACCACAAGCTAGTCTCCTTTGTGGTTTAAATGGTGGTGGACGTCGTTTGGTCCATAGATCTTCTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

348

Amino Acids

39.18

Weight (kDa)

8.3

Isoelectric Point (pI)

35.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 31 - 273 5.2e-63 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 35 - 145 7.6e-09 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 36 - 274 3.3e-08 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1021
AccII CGCG 2 cut(s) 564, 762
AciI CCGC 4 cut(s) 159, 179, 618, 885
AclI AACGTT 1 cut(s) 828
AclWI GGATC 4 cut(s) 380, 545, 558, 713
AcsI RAATTY 1 cut(s) 251
AcyI GRCGYC 1 cut(s) 1018
AfaI GTAC 3 cut(s) 23, 874, 909
AfiI CCNNNNNNNGG 1 cut(s) 25
AgsI TTSAA 6 cut(s) 13, 488, 547, 626, 653, 932
AjnI CCWGG 1 cut(s) 784
AluBI AGCT 4 cut(s) 101, 144, 586, 986
AluI AGCT 4 cut(s) 101, 144, 586, 986
Alw21I GWGCWC 2 cut(s) 103, 235
Alw26I GTCTC 2 cut(s) 45, 995
AlwI GGATC 4 cut(s) 380, 545, 558, 713
Ama87I CYCGRG 2 cut(s) 46, 96
ApeKI GCWGC 1 cut(s) 539
ApoI RAATTY 1 cut(s) 251
ArsI GACNNNNNNTTYG 2 cut(s) 824, 856
AspLEI GCGC 1 cut(s) 564
AspS9I GGNCC 3 cut(s) 222, 646, 1027
AsuHPI GGTGA 3 cut(s) 457, 710, 824
AvaI CYCGRG 2 cut(s) 46, 96
AvaII GGWCC 3 cut(s) 222, 646, 1027
BamHI GGATCC 1 cut(s) 550
BanII GRGCYC 2 cut(s) 103, 409
BarI GAAGNNNNNNTAC 2 cut(s) 480, 512
BauI CACGAG 1 cut(s) 665
BbsI GAAGAC 2 cut(s) 100, 660
Bbv12I GWGCWC 2 cut(s) 103, 235
BbvI GCAGC 1 cut(s) 526
BccI CCATC 3 cut(s) 69, 193, 302
BceAI ACGGC 1 cut(s) 182
BciT130I CCWGG 1 cut(s) 786
BcoDI GTCTC 2 cut(s) 45, 995
BfaI CTAG 2 cut(s) 104, 987
BglII AGATCT 1 cut(s) 1034
BisI GCNGC 1 cut(s) 540
BlsI GCNGC 1 cut(s) 541
Bme1390I CCNGG 1 cut(s) 786
Bme18I GGWCC 3 cut(s) 222, 646, 1027
BmeT110I CYCGRG 2 cut(s) 46, 96
BmgT120I GGNCC 3 cut(s) 222, 646, 1027
BmiI GGNNCC 1 cut(s) 552
BmrFI CCNGG 1 cut(s) 786
BmrI ACTGGG 1 cut(s) 452
BmsI GCATC 4 cut(s) 182, 526, 811, 856
BmuI ACTGGG 1 cut(s) 452
BpiI GAAGAC 2 cut(s) 100, 660
Bsa29I ATCGAT 1 cut(s) 534
BsaHI GRCGYC 1 cut(s) 1018
BsaJI CCNNGG 3 cut(s) 118, 225, 784
BsaWI WCCGGW 1 cut(s) 553
BsaXI ACNNNNNCTCC 4 cut(s) 345, 375, 886, 916
Bsc4I CCNNNNNNNGG 1 cut(s) 25
Bse118I RCCGGY 1 cut(s) 875
Bse1I ACTGG 2 cut(s) 19, 458
Bse3DI GCAATG 1 cut(s) 674
BseBI CCWGG 1 cut(s) 786
BseCI ATCGAT 1 cut(s) 534
BseDI CCNNGG 3 cut(s) 118, 225, 784
BseGI GGATG 2 cut(s) 204, 290
BseLI CCNNNNNNNGG 1 cut(s) 25
BseMI GCAATG 1 cut(s) 674
BseNI ACTGG 2 cut(s) 19, 458
BseRI GAGGAG 1 cut(s) 41
BseXI GCAGC 1 cut(s) 526
BsgI GTGCAG 3 cut(s) 157, 285, 378
Bsh1236I CGCG 2 cut(s) 564, 762
BshVI ATCGAT 1 cut(s) 534
BsiHKAI GWGCWC 2 cut(s) 103, 235
BsiHKCI CYCGRG 2 cut(s) 46, 96
BsiSI CCGG 2 cut(s) 554, 876
BslI CCNNNNNNNGG 1 cut(s) 25
BsmAI GTCTC 2 cut(s) 45, 995
BsmBI CGTCTC 1 cut(s) 45
BsmI GAATGC 1 cut(s) 161
BsoBI CYCGRG 2 cut(s) 46, 96
Bsp1286I GDGCHC 3 cut(s) 103, 235, 409
Bsp143I GATC 5 cut(s) 385, 550, 669, 718, 1034
Bsp19I CCATGG 1 cut(s) 118
BspACI CCGC 4 cut(s) 159, 179, 618, 885
BspDI ATCGAT 1 cut(s) 534
BspFNI CGCG 2 cut(s) 564, 762
BspHI TCATGA 1 cut(s) 147
BspLI GGNNCC 1 cut(s) 552
BspPI GGATC 4 cut(s) 380, 545, 558, 713
BspQI GCTCTTC 1 cut(s) 414
BsrDI GCAATG 1 cut(s) 674
BsrFI RCCGGY 1 cut(s) 875
BsrI ACTGG 2 cut(s) 19, 458
BssAI RCCGGY 1 cut(s) 875
BssECI CCNNGG 3 cut(s) 118, 225, 784
BssMI GATC 5 cut(s) 385, 550, 669, 718, 1034
BssNI GRCGYC 1 cut(s) 1018
BssSI CACGAG 1 cut(s) 665
BssT1I CCWWGG 2 cut(s) 118, 225
Bst2BI CACGAG 1 cut(s) 665
Bst2UI CCWGG 1 cut(s) 786
Bst4CI ACNGT 4 cut(s) 698, 898, 947, 959
Bst6I CTCTTC 2 cut(s) 414, 561
BstACI GRCGYC 1 cut(s) 1018
BstC8I GCNNGC 3 cut(s) 70, 171, 754
BstDEI CTNAG 1 cut(s) 632
BstDSI CCRYGG 1 cut(s) 118
BstF5I GGATG 2 cut(s) 204, 290
BstFNI CGCG 2 cut(s) 564, 762
BstHHI GCGC 1 cut(s) 564
BstKTI GATC 5 cut(s) 388, 553, 672, 721, 1037
BstMAI GTCTC 2 cut(s) 45, 995
BstMBI GATC 5 cut(s) 385, 550, 669, 718, 1034
BstMWI GCNNNNNNNGC 1 cut(s) 185
BstNI CCWGG 1 cut(s) 786
BstSCI CCNGG 1 cut(s) 784
BstUI CGCG 2 cut(s) 564, 762
BstV1I GCAGC 1 cut(s) 526
BstV2I GAAGAC 2 cut(s) 100, 660
BstX2I RGATCY 3 cut(s) 385, 550, 1034
BstXI CCANNNNNNTGG 2 cut(s) 125, 792
BstYI RGATCY 3 cut(s) 385, 550, 1034
Bsu15I ATCGAT 1 cut(s) 534
BsuTUI ATCGAT 1 cut(s) 534
BtgI CCRYGG 1 cut(s) 118
BtsCI GGATG 2 cut(s) 204, 290
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 1 cut(s) 195
Cac8I GCNNGC 3 cut(s) 70, 171, 754
CciI TCATGA 1 cut(s) 147
CfoI GCGC 1 cut(s) 564
Cfr10I RCCGGY 1 cut(s) 875
Cfr13I GGNCC 3 cut(s) 222, 646, 1027
ClaI ATCGAT 1 cut(s) 534
Csp6I GTAC 3 cut(s) 22, 873, 908
CviAII CATG 7 cut(s) 119, 148, 215, 424, 643, 797, 911
CviQI GTAC 3 cut(s) 22, 873, 908
DdeI CTNAG 1 cut(s) 632
DpnI GATC 5 cut(s) 387, 552, 671, 720, 1036
DpnII GATC 5 cut(s) 385, 550, 669, 718, 1034
DraI TTTAAA 1 cut(s) 1005
Eam1104I CTCTTC 2 cut(s) 414, 561
EarI CTCTTC 2 cut(s) 414, 561
Ecl136II GAGCTC 1 cut(s) 101
Eco130I CCWWGG 2 cut(s) 118, 225
Eco24I GRGCYC 2 cut(s) 103, 409
Eco32I GATATC 1 cut(s) 435
Eco47I GGWCC 3 cut(s) 222, 646, 1027
Eco53kI GAGCTC 1 cut(s) 101
Eco88I CYCGRG 2 cut(s) 46, 96
EcoICRI GAGCTC 1 cut(s) 101
EcoRII CCWGG 1 cut(s) 784
EcoRV GATATC 1 cut(s) 435
EcoT14I CCWWGG 2 cut(s) 118, 225
EcoT38I GRGCYC 2 cut(s) 103, 409
ErhI CCWWGG 2 cut(s) 118, 225
Esp3I CGTCTC 1 cut(s) 45
FaeI CATG 7 cut(s) 122, 151, 218, 427, 646, 800, 914
FatI CATG 7 cut(s) 118, 147, 214, 423, 642, 796, 910
FauI CCCGC 1 cut(s) 172
Fnu4HI GCNGC 1 cut(s) 540
FokI GGATG 2 cut(s) 211, 277
FriOI GRGCYC 2 cut(s) 103, 409
Fsp4HI GCNGC 1 cut(s) 540
FspBI CTAG 2 cut(s) 104, 987
GlaI GCGC 1 cut(s) 563
GluI GCNGC 1 cut(s) 540
HapII CCGG 2 cut(s) 554, 876
HhaI GCGC 1 cut(s) 564
Hin1I GRCGYC 1 cut(s) 1018
Hin1II CATG 7 cut(s) 122, 151, 218, 427, 646, 800, 914
Hin6I GCGC 1 cut(s) 562
HinP1I GCGC 1 cut(s) 562
HincII GTYRAC 2 cut(s) 832, 943
HindII GTYRAC 2 cut(s) 832, 943
HinfI GANTC 2 cut(s) 344, 881
HpaII CCGG 2 cut(s) 554, 876
HphI GGTGA 3 cut(s) 457, 710, 824
Hpy166II GTNNAC 3 cut(s) 832, 943, 1016
Hpy188I TCNGA 2 cut(s) 439, 894
Hpy188III TCNNGA 5 cut(s) 48, 148, 596, 722, 745
Hpy8I GTNNAC 3 cut(s) 832, 943, 1016
Hpy99I CGWCG 1 cut(s) 1023
HpyAV CCTTC 6 cut(s) 93, 399, 436, 553, 701, 742
HpyCH4III ACNGT 4 cut(s) 698, 898, 947, 959
HpyCH4IV ACGT 3 cut(s) 54, 828, 1018
HpyCH4V TGCA 5 cut(s) 72, 138, 188, 302, 359
HpyF10VI GCNNNNNNNGC 1 cut(s) 185
HpyF3I CTNAG 1 cut(s) 632
HpySE526I ACGT 3 cut(s) 54, 828, 1018
Hsp92I GRCGYC 1 cut(s) 1018
Hsp92II CATG 7 cut(s) 122, 151, 218, 427, 646, 800, 914
HspAI GCGC 1 cut(s) 562
Kzo9I GATC 5 cut(s) 385, 550, 669, 718, 1034
LguI GCTCTTC 1 cut(s) 414
LmnI GCTCC 1 cut(s) 424
Lsp1109I GCAGC 1 cut(s) 526
LweI GCATC 4 cut(s) 182, 526, 811, 856
MaeI CTAG 2 cut(s) 104, 987
MaeII ACGT 3 cut(s) 54, 828, 1018
MaeIII GTNAC 3 cut(s) 712, 898, 953
MalI GATC 5 cut(s) 387, 552, 671, 720, 1036
MboI GATC 5 cut(s) 385, 550, 669, 718, 1034
MfeI CAATTG 1 cut(s) 856
MflI RGATCY 3 cut(s) 385, 550, 1034
MhlI GDGCHC 3 cut(s) 103, 235, 409
MluCI AATT 2 cut(s) 251, 856
MlyI GAGTC 1 cut(s) 875
MnlI CCTC 7 cut(s) 19, 22, 122, 202, 347, 845, 931
MseI TTAA 4 cut(s) 612, 774, 803, 1004
MslI CAYNNNNRTG 1 cut(s) 123
MspA1I CMGCKG 1 cut(s) 179
MspI CCGG 2 cut(s) 554, 876
MspR9I CCNGG 1 cut(s) 786
MunI CAATTG 1 cut(s) 856
Mva1269I GAATGC 1 cut(s) 161
MvaI CCWGG 1 cut(s) 786
MvnI CGCG 2 cut(s) 564, 762
MwoI GCNNNNNNNGC 1 cut(s) 185
NcoI CCATGG 1 cut(s) 118
NdeII GATC 5 cut(s) 385, 550, 669, 718, 1034
NlaIII CATG 7 cut(s) 122, 151, 218, 427, 646, 800, 914
NlaIV GGNNCC 1 cut(s) 552
NmuCI GTSAC 2 cut(s) 712, 898
PaeR7I CTCGAG 1 cut(s) 46
PagI TCATGA 1 cut(s) 147
PciSI GCTCTTC 1 cut(s) 414
PcsI WCGNNNNNNNCGW 1 cut(s) 834
PctI GAATGC 1 cut(s) 161
PfeI GAWTC 1 cut(s) 344
PflFI GACNNNGTC 1 cut(s) 851
PkrI GCNGC 1 cut(s) 541
PleI GAGTC 1 cut(s) 875
PpsI GAGTC 1 cut(s) 875
Psp124BI GAGCTC 1 cut(s) 103
Psp1406I AACGTT 1 cut(s) 828
Psp6I CCWGG 1 cut(s) 784
PspGI CCWGG 1 cut(s) 784
PspN4I GGNNCC 1 cut(s) 552
PspPI GGNCC 3 cut(s) 222, 646, 1027
PsuI RGATCY 3 cut(s) 385, 550, 1034
PsyI GACNNNGTC 1 cut(s) 851
RsaI GTAC 3 cut(s) 23, 874, 909
RsaNI GTAC 3 cut(s) 22, 873, 908
RseI CAYNNNNRTG 1 cut(s) 123
SacI GAGCTC 1 cut(s) 103
SapI GCTCTTC 1 cut(s) 414
SaqAI TTAA 4 cut(s) 612, 774, 803, 1004
SatI GCNGC 1 cut(s) 540
Sau3AI GATC 5 cut(s) 385, 550, 669, 718, 1034
Sau96I GGNCC 3 cut(s) 222, 646, 1027
SchI GAGTC 1 cut(s) 875
ScrFI CCNGG 1 cut(s) 786
SduI GDGCHC 3 cut(s) 103, 235, 409
SfaNI GCATC 4 cut(s) 182, 526, 811, 856
Sfr274I CTCGAG 1 cut(s) 46
SinI GGWCC 3 cut(s) 222, 646, 1027
SlaI CTCGAG 1 cut(s) 46
SmiMI CAYNNNNRTG 1 cut(s) 123
SmlI CTYRAG 1 cut(s) 46
SmoI CTYRAG 1 cut(s) 46
Sse9I AATT 2 cut(s) 251, 856
SsiI CCGC 4 cut(s) 159, 179, 618, 885
SspMI CTAG 2 cut(s) 104, 987
SstI GAGCTC 1 cut(s) 103
StyD4I CCNGG 1 cut(s) 784
StyI CCWWGG 2 cut(s) 118, 225
TaaI ACNGT 4 cut(s) 698, 898, 947, 959
TaiI ACGT 3 cut(s) 57, 831, 1021
TaqI TCGA 3 cut(s) 47, 534, 744
TaqII GACCGA 1 cut(s) 210
TasI AATT 2 cut(s) 251, 856
TfiI GAWTC 1 cut(s) 344
Tru1I TTAA 4 cut(s) 612, 774, 803, 1004
Tru9I TTAA 4 cut(s) 612, 774, 803, 1004
TscAI CASTG 1 cut(s) 195
TseFI GTSAC 2 cut(s) 712, 898
TseI GCWGC 1 cut(s) 539
Tsp45I GTSAC 2 cut(s) 712, 898
TspDTI ATGAA 2 cut(s) 20, 136
TspRI CASTG 1 cut(s) 195
Tth111I GACNNNGTC 1 cut(s) 851
VpaK11BI GGWCC 3 cut(s) 222, 646, 1027
XapI RAATTY 1 cut(s) 251
XhoI CTCGAG 1 cut(s) 46
XspI CTAG 2 cut(s) 104, 987
ZraI GACGTC 1 cut(s) 1019
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.