FvH4_2g25730

Monoglyceride

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0006691
Physical Location & Seq
Reverse (-)
20784233 .. 20788867
4635 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g25730.t1

Sequence Viewer

Length: 1032 bp
ATGGACAATGAATATCAAGAAGTTTACATAAGGAATTCCAGAGGAGTGCAGCTTTTCACCTGCAGATGGTTGCCTTTCTCTTCTCCAAAGGCCCTTGTTTTCCTTTGCCATGGTTATGGCATGGAGTGCAGCGGTTTCATGAGAGAATGTGGCGTCCGGCTAGCAAATGCCGGGTATGCAGTATTCGGAATCGATTATGAGGGCCACGGGCGTTCTCGGGGGGCCAGGTGTTACATTAAGAAGTTCGAAAACATTGTTAACGATTGCTGTGACTTCTTCAAGTCGATTTGTGCGGAGGAAGAGTACAGGGACAAGTGTAGATTCTTGTATGGAGAATCCATGGGAGGGGCAGTGGCCTTATTGCTTCATAAGAAGGATCCCACATTTTGGAATGGTGCTGTTCTTGTTGCACCAATGTGCAAGATATCGGAGAAAGTAAAGCCGCATCCACTGGTTGTGAATGTATTGACCAGAGTGGAGGAGATTATACCAAAATGGAAGATTGTACCCACCAAAGACGTCATTGATTCTGCCTTCAAAGACCCTGCAAAGCGCGAAGTGATAAGAAGCAATAAACTTATCTACCAAGACAAGCCGAGGCTGAAAACAGCTTTGGAAATGCTCAGAACTAGCATGAGCCTTGAAGACAGTTTACATGAGGTGAAACTACCTTTCTTTGTTTTACATGGGGAGGCAGATACAGTTACAGACCCTGAAGTGAGCAAGTCTCTGTATAAAAAAGCCAGCAGCCCAGACAAGACCATAAAATTGTACCCTGGAATGTGGCATGGCTTGACATCAGGAGAGCCTGATGGGAACGTCGAAATCGTGTTTACAGACATCATACTTTGGCTGGAGAAGCACACTACCGATAGCAATGTTATGGATGTGCAGCCAATCGAAACATTTAATGACGGCATTGTGAAGCTGAACACCACAGCATCATCACCTGAAACCATGCACAAACATCAGCAAGGGCGCTATTTTTGTGGGCTAAAGGGACGAAGATTACAACATCACTCGGCAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 ko05225 map00561 map01100 map04714 map04723 map04923 map05225
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

344

Amino Acids

39.03

Weight (kDa)

8.2

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 28 - 270 3.6e-65 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 32 - 143 3.7e-09 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 33 - 264 6e-09 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 68
AatII GACGTC 1 cut(s) 522
Acc36I ACCTGC 1 cut(s) 68
AccB7I CCANNNNNTGG 1 cut(s) 387
AccII CGCG 1 cut(s) 555
AciI CCGC 3 cut(s) 132, 293, 443
AclWI GGATC 2 cut(s) 371, 384
AcsI RAATTY 1 cut(s) 34
AcuI CTGAAG 1 cut(s) 735
AcyI GRCGYC 2 cut(s) 153, 519
AfaI GTAC 3 cut(s) 305, 507, 773
AfiI CCNNNNNNNGG 3 cut(s) 66, 345, 387
AgsI TTSAA 3 cut(s) 280, 538, 644
AjnI CCWGG 2 cut(s) 224, 775
AjuI GAANNNNNNNTTGG 2 cut(s) 596, 628
AleI CACNNNNGTG 1 cut(s) 415
AluBI AGCT 3 cut(s) 52, 611, 928
AluI AGCT 3 cut(s) 52, 611, 928
Alw26I GTCTC 1 cut(s) 732
AlwI GGATC 2 cut(s) 371, 384
AlwNI CAGNNNCTG 1 cut(s) 713
Ama87I CYCGRG 1 cut(s) 216
AoxI GGCC 4 cut(s) 90, 202, 222, 354
ApeKI GCWGC 4 cut(s) 49, 129, 747, 892
ApoI RAATTY 1 cut(s) 34
AspLEI GCGC 2 cut(s) 555, 981
AspS9I GGNCC 3 cut(s) 91, 202, 222
AsuC2I CCSGG 1 cut(s) 172
AsuHPI GGTGA 3 cut(s) 49, 673, 939
AsuII TTCGAA 1 cut(s) 246
AsuNHI GCTAGC 1 cut(s) 160
AvaI CYCGRG 1 cut(s) 216
BamHI GGATCC 1 cut(s) 376
BarI GAAGNNNNNNTAC 2 cut(s) 636, 668
BbsI GAAGAC 1 cut(s) 651
BbvI GCAGC 4 cut(s) 61, 141, 759, 904
BccI CCATC 2 cut(s) 60, 806
BceAI ACGGC 1 cut(s) 931
BciT130I CCWGG 2 cut(s) 226, 777
BcnI CCSGG 1 cut(s) 172
BcoDI GTCTC 1 cut(s) 732
BfaI CTAG 2 cut(s) 161, 630
BfmI CTRYAG 1 cut(s) 61
BfoI RGCGCY 1 cut(s) 982
BfuAI ACCTGC 1 cut(s) 68
BisI GCNGC 5 cut(s) 50, 130, 443, 748, 893
BlsI GCNGC 5 cut(s) 51, 131, 444, 749, 894
Bme1390I CCNGG 3 cut(s) 172, 226, 777
BmeT110I CYCGRG 1 cut(s) 216
BmgT120I GGNCC 3 cut(s) 91, 202, 222
BmiI GGNNCC 2 cut(s) 223, 378
BmrFI CCNGG 3 cut(s) 172, 226, 777
BmsI GCATC 2 cut(s) 454, 950
BmtI GCTAGC 1 cut(s) 164
BpiI GAAGAC 1 cut(s) 651
BplI GAGNNNNNCTC 2 cut(s) 712, 744
BpmI CTGGAG 1 cut(s) 875
Bpu14I TTCGAA 1 cut(s) 246
BpuMI CCSGG 1 cut(s) 172
Bsa29I ATCGAT 1 cut(s) 192
BsaHI GRCGYC 2 cut(s) 153, 519
BsaJI CCNNGG 5 cut(s) 109, 205, 339, 596, 775
BsaXI ACNNNNNCTCC 4 cut(s) 287, 317, 336, 366
Bsc4I CCNNNNNNNGG 3 cut(s) 66, 345, 387
Bse1I ACTGG 1 cut(s) 456
Bse3DI GCAATG 2 cut(s) 883, 1032
BseBI CCWGG 2 cut(s) 226, 777
BseCI ATCGAT 1 cut(s) 192
BseDI CCNNGG 5 cut(s) 109, 205, 339, 596, 775
BseGI GGATG 2 cut(s) 445, 892
BseLI CCNNNNNNNGG 3 cut(s) 66, 345, 387
BseMI GCAATG 2 cut(s) 883, 1032
BseMII CTCAG 1 cut(s) 637
BseNI ACTGG 1 cut(s) 456
BseRI GAGGAG 2 cut(s) 57, 494
BseXI GCAGC 4 cut(s) 61, 141, 759, 904
BsgI GTGCAG 3 cut(s) 68, 148, 911
Bsh1236I CGCG 1 cut(s) 555
BshFI GGCC 4 cut(s) 92, 204, 224, 356
BshVI ATCGAT 1 cut(s) 192
BsiHKCI CYCGRG 1 cut(s) 216
BsiSI CCGG 2 cut(s) 157, 171
BslFI GGGAC 2 cut(s) 323, 1014
BslI CCNNNNNNNGG 3 cut(s) 66, 345, 387
BsmAI GTCTC 1 cut(s) 732
BsmFI GGGAC 2 cut(s) 323, 1014
BsnI GGCC 4 cut(s) 92, 204, 224, 356
BsoBI CYCGRG 1 cut(s) 216
Bsp119I TTCGAA 1 cut(s) 246
Bsp143I GATC 1 cut(s) 376
Bsp19I CCATGG 2 cut(s) 109, 339
BspACI CCGC 3 cut(s) 132, 293, 443
BspANI GGCC 4 cut(s) 92, 204, 224, 356
BspCNI CTCAG 1 cut(s) 636
BspDI ATCGAT 1 cut(s) 192
BspFNI CGCG 1 cut(s) 555
BspHI TCATGA 1 cut(s) 138
BspLI GGNNCC 2 cut(s) 223, 378
BspMAI CTGCAG 1 cut(s) 65
BspMI ACCTGC 1 cut(s) 68
BspOI GCTAGC 1 cut(s) 164
BspPI GGATC 2 cut(s) 371, 384
BspT104I TTCGAA 1 cut(s) 246
BsrDI GCAATG 2 cut(s) 883, 1032
BsrI ACTGG 1 cut(s) 456
BssECI CCNNGG 5 cut(s) 109, 205, 339, 596, 775
BssMI GATC 1 cut(s) 376
BssNI GRCGYC 2 cut(s) 153, 519
BssT1I CCWWGG 2 cut(s) 109, 339
Bst2UI CCWGG 2 cut(s) 226, 777
Bst4CI ACNGT 2 cut(s) 650, 703
Bst6I CTCTTC 2 cut(s) 85, 294
BstACI GRCGYC 2 cut(s) 153, 519
BstAPI GCANNNNNTGC 1 cut(s) 126
BstBI TTCGAA 1 cut(s) 246
BstC8I GCNNGC 2 cut(s) 162, 745
BstDEI CTNAG 1 cut(s) 623
BstDSI CCRYGG 3 cut(s) 109, 205, 339
BstF5I GGATG 2 cut(s) 445, 892
BstFNI CGCG 1 cut(s) 555
BstH2I RGCGCY 1 cut(s) 982
BstHHI GCGC 2 cut(s) 555, 981
BstKTI GATC 1 cut(s) 379
BstMAI GTCTC 1 cut(s) 732
BstMBI GATC 1 cut(s) 376
BstMWI GCNNNNNNNGC 3 cut(s) 126, 176, 859
BstNI CCWGG 2 cut(s) 226, 777
BstSCI CCNGG 3 cut(s) 170, 224, 775
BstSFI CTRYAG 1 cut(s) 61
BstUI CGCG 1 cut(s) 555
BstV1I GCAGC 4 cut(s) 61, 141, 759, 904
BstV2I GAAGAC 1 cut(s) 651
BstX2I RGATCY 1 cut(s) 376
BstXI CCANNNNNNTGG 1 cut(s) 116
BstYI RGATCY 1 cut(s) 376
Bsu15I ATCGAT 1 cut(s) 192
BsuRI GGCC 4 cut(s) 92, 204, 224, 356
BsuTUI ATCGAT 1 cut(s) 192
BtgI CCRYGG 3 cut(s) 109, 205, 339
BtsCI GGATG 2 cut(s) 445, 892
BtsI GCAGTG 1 cut(s) 357
BtsIMutI CAGTG 2 cut(s) 357, 449
BveI ACCTGC 1 cut(s) 68
Cac8I GCNNGC 2 cut(s) 162, 745
CaiI CAGNNNCTG 1 cut(s) 713
CciI TCATGA 1 cut(s) 138
CfoI GCGC 2 cut(s) 555, 981
Cfr13I GGNCC 3 cut(s) 91, 202, 222
ClaI ATCGAT 1 cut(s) 192
CseI GACGC 1 cut(s) 142
Csp6I GTAC 3 cut(s) 304, 506, 772
CviAII CATG 9 cut(s) 110, 121, 139, 340, 634, 656, 686, 788, 958
CviQI GTAC 3 cut(s) 304, 506, 772
DdeI CTNAG 1 cut(s) 623
DpnI GATC 1 cut(s) 378
DpnII GATC 1 cut(s) 376
Eam1104I CTCTTC 2 cut(s) 85, 294
EarI CTCTTC 2 cut(s) 85, 294
Eco130I CCWWGG 2 cut(s) 109, 339
Eco32I GATATC 1 cut(s) 426
Eco57I CTGAAG 1 cut(s) 735
Eco88I CYCGRG 1 cut(s) 216
EcoO109I RGGNCCY 1 cut(s) 91
EcoRI GAATTC 1 cut(s) 34
EcoRII CCWGG 2 cut(s) 224, 775
EcoRV GATATC 1 cut(s) 426
EcoT14I CCWWGG 2 cut(s) 109, 339
ErhI CCWWGG 2 cut(s) 109, 339
FaeI CATG 9 cut(s) 113, 124, 142, 343, 637, 659, 689, 791, 961
FaqI GGGAC 2 cut(s) 323, 1014
FatI CATG 9 cut(s) 109, 120, 138, 339, 633, 655, 685, 787, 957
Fnu4HI GCNGC 5 cut(s) 50, 130, 443, 748, 893
FokI GGATG 2 cut(s) 432, 899
Fsp4HI GCNGC 5 cut(s) 50, 130, 443, 748, 893
FspBI CTAG 2 cut(s) 161, 630
GlaI GCGC 2 cut(s) 554, 980
GluI GCNGC 5 cut(s) 50, 130, 443, 748, 893
GsuI CTGGAG 1 cut(s) 875
HaeII RGCGCY 1 cut(s) 982
HaeIII GGCC 4 cut(s) 92, 204, 224, 356
HapII CCGG 2 cut(s) 157, 171
HgaI GACGC 1 cut(s) 142
HhaI GCGC 2 cut(s) 555, 981
Hin1I GRCGYC 2 cut(s) 153, 519
Hin1II CATG 9 cut(s) 113, 124, 142, 343, 637, 659, 689, 791, 961
Hin6I GCGC 2 cut(s) 553, 979
HinP1I GCGC 2 cut(s) 553, 979
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HinfI GANTC 4 cut(s) 189, 321, 335, 527
HpaI GTTAAC 1 cut(s) 259
HpaII CCGG 2 cut(s) 157, 171
HphI GGTGA 3 cut(s) 49, 673, 939
Hpy166II GTNNAC 4 cut(s) 25, 259, 653, 834
Hpy188I TCNGA 3 cut(s) 188, 430, 626
Hpy188III TCNNGA 4 cut(s) 17, 39, 139, 801
Hpy8I GTNNAC 4 cut(s) 25, 259, 653, 834
Hpy99I CGWCG 1 cut(s) 824
HpyAV CCTTC 2 cut(s) 367, 544
HpyCH4III ACNGT 2 cut(s) 650, 703
HpyCH4IV ACGT 2 cut(s) 519, 819
HpyCH4V TGCA 9 cut(s) 49, 63, 129, 179, 410, 420, 548, 892, 961
HpyF10VI GCNNNNNNNGC 3 cut(s) 126, 176, 859
HpyF3I CTNAG 1 cut(s) 623
HpySE526I ACGT 2 cut(s) 519, 819
Hsp92I GRCGYC 2 cut(s) 153, 519
Hsp92II CATG 9 cut(s) 113, 124, 142, 343, 637, 659, 689, 791, 961
HspAI GCGC 2 cut(s) 553, 979
KspAI GTTAAC 1 cut(s) 259
Kzo9I GATC 1 cut(s) 376
Lsp1109I GCAGC 4 cut(s) 61, 141, 759, 904
LweI GCATC 2 cut(s) 454, 950
MaeI CTAG 2 cut(s) 161, 630
MaeII ACGT 2 cut(s) 519, 819
MaeIII GTNAC 3 cut(s) 230, 269, 703
MalI GATC 1 cut(s) 378
MboI GATC 1 cut(s) 376
MboII GAAGA 6 cut(s) 72, 268, 311, 511, 656, 1017
MflI RGATCY 1 cut(s) 376
MluCI AATT 2 cut(s) 34, 767
MnlI CCTC 8 cut(s) 35, 193, 289, 338, 472, 591, 652, 685
MseI TTAA 3 cut(s) 237, 258, 909
MslI CAYNNNNRTG 2 cut(s) 114, 415
MspA1I CMGCKG 1 cut(s) 132
MspI CCGG 2 cut(s) 157, 171
MspR9I CCNGG 3 cut(s) 172, 226, 777
MvaI CCWGG 2 cut(s) 226, 777
MvnI CGCG 1 cut(s) 555
MwoI GCNNNNNNNGC 3 cut(s) 126, 176, 859
NciI CCSGG 1 cut(s) 172
NcoI CCATGG 2 cut(s) 109, 339
NdeII GATC 1 cut(s) 376
NheI GCTAGC 1 cut(s) 160
NlaIII CATG 9 cut(s) 113, 124, 142, 343, 637, 659, 689, 791, 961
NlaIV GGNNCC 2 cut(s) 223, 378
NmeAIII GCCGAG 2 cut(s) 621, 1001
NmuCI GTSAC 1 cut(s) 269
NspV TTCGAA 1 cut(s) 246
OliI CACNNNNGTG 1 cut(s) 415
PagI TCATGA 1 cut(s) 138
PaqCI CACCTGC 1 cut(s) 68
PcsI WCGNNNNNNNCGW 1 cut(s) 825
PfeI GAWTC 4 cut(s) 189, 321, 335, 527
PflMI CCANNNNNTGG 1 cut(s) 387
PkrI GCNGC 5 cut(s) 51, 131, 444, 749, 894
Psp6I CCWGG 2 cut(s) 224, 775
PspGI CCWGG 2 cut(s) 224, 775
PspN4I GGNNCC 2 cut(s) 223, 378
PspPI GGNCC 3 cut(s) 91, 202, 222
PstI CTGCAG 1 cut(s) 65
PstNI CAGNNNCTG 1 cut(s) 713
PsuI RGATCY 1 cut(s) 376
RsaI GTAC 3 cut(s) 305, 507, 773
RsaNI GTAC 3 cut(s) 304, 506, 772
RseI CAYNNNNRTG 2 cut(s) 114, 415
SaqAI TTAA 3 cut(s) 237, 258, 909
SatI GCNGC 5 cut(s) 50, 130, 443, 748, 893
Sau3AI GATC 1 cut(s) 376
Sau96I GGNCC 3 cut(s) 91, 202, 222
ScrFI CCNGG 3 cut(s) 172, 226, 777
SfaNI GCATC 2 cut(s) 454, 950
SfcI CTRYAG 1 cut(s) 61
SfuI TTCGAA 1 cut(s) 246
SmiMI CAYNNNNRTG 2 cut(s) 114, 415
Sse9I AATT 2 cut(s) 34, 767
SsiI CCGC 3 cut(s) 132, 293, 443
SspMI CTAG 2 cut(s) 161, 630
StyD4I CCNGG 3 cut(s) 170, 224, 775
StyI CCWWGG 2 cut(s) 109, 339
TaaI ACNGT 2 cut(s) 650, 703
TaiI ACGT 2 cut(s) 522, 822
TaqI TCGA 5 cut(s) 192, 246, 284, 822, 900
TasI AATT 2 cut(s) 34, 767
TatI WGTACW 1 cut(s) 303
TauI GCSGC 1 cut(s) 445
TfiI GAWTC 4 cut(s) 189, 321, 335, 527
Tru1I TTAA 3 cut(s) 237, 258, 909
Tru9I TTAA 3 cut(s) 237, 258, 909
TscAI CASTG 2 cut(s) 357, 456
TseFI GTSAC 1 cut(s) 269
TseI GCWGC 4 cut(s) 49, 129, 747, 892
Tsp45I GTSAC 1 cut(s) 269
TspDTI ATGAA 3 cut(s) 24, 127, 356
TspRI CASTG 2 cut(s) 357, 456
Van91I CCANNNNNTGG 1 cut(s) 387
XapI RAATTY 1 cut(s) 34
XspI CTAG 2 cut(s) 161, 630
ZraI GACGTC 1 cut(s) 520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.